CN114052045A - Disinfecting composition, disinfecting liquid, application and preparation method - Google Patents
Disinfecting composition, disinfecting liquid, application and preparation method Download PDFInfo
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- CN114052045A CN114052045A CN202010757290.XA CN202010757290A CN114052045A CN 114052045 A CN114052045 A CN 114052045A CN 202010757290 A CN202010757290 A CN 202010757290A CN 114052045 A CN114052045 A CN 114052045A
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- fructus forsythiae
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/12—Quaternary ammonium compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
Abstract
The invention relates to the technical field of indoor disinfection, in particular to a disinfection composition and application thereof, and a disinfectant prepared from the disinfection composition and a preparation method thereof. The disinfection composition comprises the following components in parts by weight: 0.1-1 part of fructus forsythiae extract, 0.1-1 part of honeysuckle extract, 0.05-0.15 part of didecyl dimethyl ammonium chloride, 0.1-0.5 part of poly aminopropyl biguanide and 0.05-0.5 part of benzalkonium chloride. The disinfection composition has excellent sterilization effect, mild property, no stimulation to skin and no corrosion to metal, and can be prepared into disinfection solution for household daily disinfection.
Description
Technical Field
The invention relates to the technical field of indoor disinfection, in particular to a disinfection composition and application thereof, and a disinfectant prepared from the disinfection composition and a preparation method thereof.
Background
The biological pollution in the indoor environment seriously threatens the health of people and can cause various diseases, such as various respiratory infectious diseases, asthma, building syndrome and the like. Bacteria and fungi (including fungal spores) are indoor biological pollutants, generally exist on the surfaces of objects which are frequently contacted daily, such as furniture, sanitary wares, kitchenware, cleaning tools, floors, walls and the like, and can be greatly propagated in warm and humid environments. Such as: the kitchen sink is wet and often contains garbage, and bacteria with pathogenic ability can grow in hidden gaps; the faucet and its surroundings are often wet by running water, which is also an ideal place for bacterial growth. When bacteria are attached to a faucet or a water tank through hands, food or the like, the bacteria can be greatly propagated in the faucet or the water tank, and finally a microbial film can be formed and attached to the bacteria. For another example: the door mat always contacts the sole, often gathers a large amount of escherichia coli and other multiple pathogenic bacteria, and can bring the pathogenic bacteria into the room after stepping on the door mat each time. The following steps are repeated: after the washing machine is used for washing clothes, if the clothes are not cleaned and dried in time, the clothes are easy to store dirt and breed bacteria, and the clothes can be polluted secondarily when the clothes are washed. In addition, bacteria may be suspended in the air by forming an aerosol through the air flow, causing biological contamination of the indoor air. Thus, a person in a biologically contaminated indoor environment may be subject to bacterial attack by contact or breathing, among other means. Studies have shown that even with uv radiation, a small amount of bacteria remains in the room.
The disinfection solution commonly used in the family environment at present comprises 75% medical alcohol, 84 disinfection solution, peroxyacetic acid and the like. However, 75% medical alcohol can enter bacteria, but can not solidify protein in the body, so the bacteria can not be killed completely, and the alcohol has strong taste and large irritation, is not suitable for large-area household disinfection, is inflammable, and has potential safety hazard during storage; 84 has pungent smell and strong corrosive action, can burn respiratory mucosa, can cause discomfort for people when used indoors, and 84 has bleaching property and corrosivity, can cause color change of furniture, metal products and textiles, and is not suitable for disinfection of colored fabrics or metal products; peracetic acid is a strong oxidant, is extremely unstable, flammable and explosive, has strong corrosivity, has certain potential safety hazards in storage and use processes, and can cause certain damage and corrosion to skin, colored fabrics and metal products.
In addition, surfactants, builders and other chemical additives added to commercially available disinfecting products can damage the oily protective layer on the skin surface, thus aggravating the skin's corrosion and damage caused by the disinfecting products, which often contact the skin to cause dryness, roughness and even breakage, and also cause various degrees of damage to the hair and other organs of the human body.
Disclosure of Invention
Aiming at the problems of corrosivity and skin irritation of the existing indoor disinfection products, the invention provides a disinfection composition, a disinfection solution, application and a preparation method.
In order to achieve the purpose of the invention, the embodiment of the invention adopts the following technical scheme:
in one aspect, the embodiment of the invention provides a disinfection composition, which comprises the following components in parts by weight: 0.1-1 part of fructus forsythiae extract, 0.1-1 part of honeysuckle extract, 0.05-0.15 part of didecyl dimethyl ammonium chloride, 0.1-0.5 part of poly aminopropyl biguanide and 0.05-0.5 part of benzalkonium chloride.
In the sterilizing composition, benzalkonium chloride has the advantages of wide sterilization spectrum, strong bactericidal power, small irritation to skin and tissues and no corrosion to metal and rubber products. Benzalkonium chloride can change the permeability of bacterial cytoplasm membrane to make the bacterial cytoplasm substance exosmose and make its metabolism blocked, thereby playing the role of killing bacteria. The polyaminopropyl biguanide destroys normal substance and energy metabolism by interacting with groups or elements in organisms, and is easily adsorbed by bacteria and viruses to inhibit the division function of the bacteria and viruses. The two are used in combination, so that the effects of the two on the division and metabolism of bacteria can be enhanced, the bacteria can be killed more quickly and effectively, and the propagation of the bacteria can be inhibited.
However, benzalkonium chloride binds protein more rapidly and thus has a reduced bactericidal effect when exposed to cotton, cellulose and other organic substances. The didecyl dimethyl ammonium chloride has excellent surface activity and wetting property, can still play a high-efficiency bactericidal effect under the condition of organic dirt, and is beneficial to maintaining the bactericidal activity of the benzalkonium chloride, so that the composition has high bactericidal efficiency and long bacteriostatic action. The didecyl dimethyl ammonium chloride is a broad-spectrum and safe bactericidal substance, and has excellent biological killing effect on gram-positive bacteria, gram-negative bacteria, mildew, viruses, algae, enveloped viruses such as hepatitis B, HIV and the like, so that the excellent disinfection effect can be achieved by combining the didecyl dimethyl ammonium chloride, the polyaminopropyl biguanide and the benzalkonium chloride.
The fructus forsythiae extractive solution has strong inhibitory effect on Staphylococcus aureus and Shigella dysenteriae, and also has inhibitory effect on Bacillus typhi, Vibrio cholerae, Escherichia coli, Pseudomonas aeruginosa, Bordetella pertussis, Mycobacterium tuberculosis, Diplococcus pneumoniae, hemolytic streptococcus and influenza virus, and antifungal effect. The flos Lonicerae extractive solution has certain inhibitory effect on various pathogenic bacteria such as Staphylococcus aureus, hemolytic streptococcus, Escherichia coli, Shigella dysenteriae, Vibrio cholerae, typhoid bacillus, and paratyphoid bacillus, has inhibitory and killing effects on pneumococcus, meningococcus, Pseudomonas aeruginosa, Mycobacterium tuberculosis, Shigella shigella, Streptococcus mutans, etc., and can inhibit influenza virus, orphan virus, herpes virus, and leptospira. The invention discovers in research that the fructus forsythiae extract and the honeysuckle extract have the effects of repairing skin, improving cell activity and maintaining normal states of elastic fibers, collagen fibers and proteoglycan, and the disinfection composition prepared by compounding the fructus forsythiae extract and the honeysuckle extract with didecyl dimethyl ammonium chloride, polyaminopropyl biguanide and benzalkonium chloride within the dosage range has excellent disinfection and bacteriostasis effects, can also obviously reduce stimulation and damage of the benzalkonium chloride, the polyaminopropyl biguanide and the didecyl dimethyl ammonium chloride to skin, and is milder to skin compared with the disinfection composition prepared by singly using the benzalkonium chloride, the polyaminopropyl biguanide and the didecyl dimethyl ammonium chloride. In addition, the disinfecting composition is free from bleaching and other damaging effects on furniture and textiles, and is non-corrosive to metal articles.
Preferably, the disinfecting composition comprises the following components in parts by weight: 0.1-0.3 part of fructus forsythiae extract, 0.1-0.3 part of honeysuckle extract, 0.05-0.1 part of didecyl dimethyl ammonium chloride, 0.3-0.5 part of poly aminopropyl biguanide and 0.05-0.15 part of benzalkonium chloride.
Preferably, the disinfecting composition comprises the following components in parts by weight: 0.1 part of fructus forsythiae extract, 0.1 part of honeysuckle extract, 0.1 part of didecyl dimethyl ammonium chloride, 0.4 part of poly aminopropyl biguanide and 0.1 part of benzalkonium chloride. The disinfectant composition prepared according to the proportion has a more excellent disinfecting effect, does not irritate skin, and does not damage furniture, textiles and the like.
Optionally, the preparation method of the forsythia suspense extract comprises the following steps: heating and refluxing fructus forsythiae by using 45-65% v/v ethanol water solution with the pH value adjusted to 8-8.5 by using sodium hydroxide; filtering after extraction is finished, concentrating the obtained filtrate, applying polar macroporous adsorption resin to the filtrate, eluting with 85-95% v/v ethanol water solution, eluting with water, collecting the elution fraction, and concentrating to 1.2-1.3 g/ml to obtain the fructus forsythiae extracting solution; wherein the mass of the 45-65% v/v ethanol water solution is 5-8 times of that of the fructus forsythiae.
The forsythia suspense extract obtained by the preparation method can further improve the disinfection effect of the disinfection composition, and the irritation of the disinfection composition can be remarkably reduced by combining the forsythia suspense extract with the honeysuckle extract. The polar macroporous adsorbent resin is preferably XDA-1, XDA-8, LSA-7 or LSA-5B.
Optionally, the preparation method of the honeysuckle extract comprises the following steps: and (2) heating and refluxing the honeysuckle by using 55-60% v/v ethanol water solution with the pH value adjusted to 8-8.5 by using sodium hydroxide, filtering, heating and refluxing the obtained filter residue by using 25-35% v/v ethanol water solution, filtering, combining filtrates, and concentrating to 1.2-1.3 g/ml to obtain the honeysuckle extracting solution.
The honeysuckle extract obtained by the preparation method has better disinfection effect and the effect of reducing the irritation of the disinfection composition, and the effects of improving the disinfection effect and reducing the irritation can be further improved after the honeysuckle extract is combined with the forsythia extract obtained by the preparation method.
In a second aspect, embodiments of the present invention also provide the use of a disinfecting composition as described above in the manufacture of a household disinfecting product for daily use.
The disinfection composition has excellent disinfection effect, is mild, safe and non-irritant to skin, has no damage to furniture and textiles and no corrosion to metal products, and can be prepared into household daily disinfection products such as disinfection spray, disinfection wet tissues, disinfection liquid and the like.
In a third aspect, the embodiment of the present invention further provides a disinfection solution, including the above disinfection composition. The disinfectant can be filled in a spraying device to be used as a spraying agent for household daily disinfection, and can also be directly used for scrubbing furniture and sanitary wares in a household environment, or for soaking textiles such as ground mats, pillows, bed sheets and the like.
The disinfectant comprises the following components in parts by weight:
the humectant is selected from at least one of propylene glycol, glycerin and butylene glycol;
the preservative is phenoxyethanol;
the solubilizer is PEG-40 hydrogenated castor oil;
the antioxidant is ethylene diamine tetraacetic acid disodium salt (EDTA disodium).
The above preferred raw materials are all the conventional components for the daily chemical products, have high safety, and do not cause obvious discomfort to the skin when used in the above dosage range. The citric acid can also enhance the disinfection performance of benzalkonium chloride, polyaminopropyl biguanide and didecyl dimethyl ammonium chloride, and the citric acid also has a certain sterilization effect, so the disinfection effect of the disinfection composition can be improved.
In a fourth aspect, the present invention further provides a method for preparing the disinfectant solution, including the following steps:
adding the humectant into the water, and stirring until the humectant is dissolved;
adding the didecyl dimethyl ammonium chloride, the polyaminopropyl biguanide and the benzalkonium chloride, and stirring and dispersing uniformly;
adding the fructus forsythiae extract, the honeysuckle extract, the citric acid, the essence, the solubilizer, the antioxidant and the preservative, and uniformly mixing to obtain the disinfectant.
The preferable temperature for adding the humectant is 75-85 ℃, and the temperature range can accelerate the dissolving of the humectant and improve the production efficiency.
The solution temperature when the didecyl dimethyl ammonium chloride, the polyaminopropyl biguanide and the benzalkonium chloride are added is preferably 48-52 ℃, the temperature range can accelerate the dissolution of the components, and the components are prevented from being decomposed at high temperature or from reacting with each other to generate impurities.
The temperature of the solution when the fructus forsythiae extracting solution, the honeysuckle extracting solution, the citric acid, the essence, the solubilizer, the antioxidant and the preservative are added is preferably 43-47 ℃, the temperature range can promote the fructus forsythiae extracting solution and the honeysuckle extracting solution to be uniformly mixed with other components under the assistance of the solubilizer, and the phenomenon that the citric acid, the essence, the antioxidant and the preservative are decomposed at a high temperature to lose efficacy is avoided.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The experimental procedures used in the following examples are all conventional in the art unless otherwise specified.
The forsythia and the honeysuckle used in the following examples all meet the relevant regulations under each medicinal material item in the text of the Chinese pharmacopoeia (2015 edition).
The raw materials, reagents and the like used in the following examples were obtained commercially unless otherwise specified.
Example 1
The embodiment of the invention provides a disinfection composition, which comprises the following components in parts by weight:
0.1 part of fructus forsythiae extract, 0.1 part of honeysuckle extract, 0.1 part of didecyl dimethyl ammonium chloride, 0.4 part of poly aminopropyl biguanide and 0.1 part of benzalkonium chloride.
The preparation method of the fructus forsythiae extracting solution comprises the following steps: heating and refluxing fructus forsythiae by 55% v/v ethanol water solution with pH adjusted to 8.2 by sodium hydroxide, wherein the mass of the 55% v/v ethanol water solution is 7 times that of fructus forsythiae; filtering after extraction, concentrating the obtained filtrate, loading on XDA-1 macroporous adsorbent resin, eluting with 90% v/v ethanol water solution, eluting with water, collecting eluate, and concentrating to 1.25 g/ml;
the preparation method of the honeysuckle extract comprises the following steps: heating and refluxing the honeysuckle by using a 57% v/v ethanol water solution with the pH value adjusted to 8.2 by using sodium hydroxide, wherein the mass of the 57% v/v ethanol water solution is 7 times that of the honeysuckle; filtering, and performing heating reflux extraction on the obtained filter residue by using 30% v/v ethanol water solution, wherein the mass of the 30% v/v ethanol water solution is 7 times that of the honeysuckle; filtering, mixing the filtrates, and concentrating to 1.25 g/ml.
Example 2
The embodiment of the invention provides a disinfection composition, which comprises the following components in parts by weight:
0.1 part of fructus forsythiae extract, 0.1 part of honeysuckle extract, 0.1 part of didecyl dimethyl ammonium chloride, 0.4 part of poly aminopropyl biguanide and 0.1 part of benzalkonium chloride.
The preparation method of the fructus forsythiae extracting solution comprises the following steps: heating and refluxing fructus forsythiae by 45% v/v ethanol water solution with pH adjusted to 8 by sodium hydroxide, wherein the mass of the 45% v/v ethanol water solution is 5 times of that of fructus forsythiae; filtering after extraction, concentrating the obtained filtrate, loading on XDA-8 macroporous adsorbent resin, eluting with 85% v/v ethanol water solution, eluting with water, collecting eluate, and concentrating to 1.2 g/ml;
the preparation method of the honeysuckle extract comprises the following steps: heating and refluxing the honeysuckle by 55% v/v ethanol water solution with the pH value adjusted to 8 by sodium hydroxide, wherein the mass of the 55% v/v ethanol water solution is 6 times that of the honeysuckle; filtering, and performing heating reflux extraction on the obtained filter residue by using a 25% v/v ethanol water solution, wherein the mass of the 25% v/v ethanol water solution is 6 times that of the honeysuckle; filtering, mixing the filtrates, and concentrating to 1.2 g/ml.
Example 3
The embodiment of the invention provides a disinfection composition, which comprises the following components in parts by weight:
0.1 part of fructus forsythiae extract, 0.1 part of honeysuckle extract, 0.1 part of didecyl dimethyl ammonium chloride, 0.4 part of poly aminopropyl biguanide and 0.1 part of benzalkonium chloride.
The preparation method of the fructus forsythiae extracting solution comprises the following steps: heating and refluxing fructus forsythiae by 65% v/v ethanol water solution with pH adjusted to 8.5 by sodium hydroxide, wherein the mass of the 65% v/v ethanol water solution is 8 times of that of fructus forsythiae; filtering after extraction, concentrating the obtained filtrate, loading on LSA-7 macroporous adsorbent resin, eluting with 95% v/v ethanol water solution, eluting with water, collecting eluate fraction, and concentrating to 1.3g/ml to obtain the final product;
the preparation method of the honeysuckle extract comprises the following steps: heating and refluxing the honeysuckle by using 60% v/v ethanol water solution with the pH value adjusted to 8.5 by using sodium hydroxide, wherein the mass of the 60% v/v ethanol water solution is 8 times that of the honeysuckle; filtering, and heating and refluxing the obtained filter residue by using 35% v/v ethanol water solution, wherein the mass of the 35% v/v ethanol water solution is 8 times that of the honeysuckle; filtering, mixing the filtrates, and concentrating to 1.3 g/ml.
Example 4
The embodiment of the invention provides a disinfection composition, which comprises the following components in parts by weight:
0.3 part of fructus forsythiae extract, 0.3 part of honeysuckle extract, 0.05 part of didecyl dimethyl ammonium chloride, 0.3 part of poly aminopropyl biguanide and 0.05 part of benzalkonium chloride.
The preparation method of fructus forsythiae extract and flos Lonicerae extract is the same as example 1.
Example 5
The embodiment of the invention provides a disinfection composition, which comprises the following components in parts by weight:
0.3 part of fructus forsythiae extract, 0.3 part of honeysuckle extract, 0.1 part of didecyl dimethyl ammonium chloride, 0.4 part of poly aminopropyl biguanide and 0.05 part of benzalkonium chloride.
The preparation method of fructus forsythiae extract and flos Lonicerae extract is the same as example 1.
Example 6
The embodiment of the invention provides a disinfection composition, which comprises the following components in parts by weight:
0.1 part of fructus forsythiae extract, 0.1 part of honeysuckle extract, 0.05 part of didecyl dimethyl ammonium chloride, 0.5 part of poly aminopropyl biguanide and 0.15 part of benzalkonium chloride.
The preparation method of fructus forsythiae extract and flos Lonicerae extract is the same as example 1.
Example 7
The embodiment of the invention provides a disinfection composition, which comprises the following components in parts by weight:
0.1 part of fructus forsythiae extract, 0.1 part of honeysuckle extract, 0.05 part of didecyl dimethyl ammonium chloride, 0.1 part of poly-aminopropyl biguanide and 0.05 part of benzalkonium chloride.
Example 8
The embodiment of the invention provides a disinfection composition, which comprises the following components in parts by weight:
1 part of fructus forsythiae extract, 1 part of honeysuckle extract, 0.15 part of didecyl dimethyl ammonium chloride, 0.5 part of poly-aminopropyl biguanide and 0.5 part of benzalkonium chloride.
Example 9
The embodiment of the invention provides a household daily disinfectant and a preparation method thereof, wherein the disinfectant comprises the following components in parts by weight:
the preparation method of fructus forsythiae extract and flos Lonicerae extract is the same as example 1.
The preparation method of the disinfectant comprises the following steps:
adding propylene glycol into water, heating to 80 ℃, and stirring to completely dissolve;
cooling to 50 deg.C, adding didecyl dimethyl ammonium chloride, polyaminopropyl biguanide and benzalkonium chloride, stirring and dispersing;
cooling to 45 deg.C, adding fructus forsythiae extractive solution and flos Lonicerae extractive solution, citric acid, essence, PEG-40 hydrogenated castor oil, EDTA disodium and phenoxyethanol;
cooling to 40 ℃ to obtain the disinfectant.
Example 10
The embodiment of the invention provides a household daily disinfectant and a preparation method thereof, wherein the disinfectant comprises the following components in parts by weight:
the preparation method of fructus forsythiae extract and flos Lonicerae extract is the same as that of example 2.
The preparation method of the disinfectant comprises the following steps:
adding butanediol into water, heating to 75 ℃, and stirring to completely dissolve;
cooling to 48 deg.C, adding didecyl dimethyl ammonium chloride, polyaminopropyl biguanide and benzalkonium chloride, stirring and dispersing;
cooling to 43 deg.C, adding fructus forsythiae extractive solution and flos Lonicerae extractive solution, citric acid, essence, PEG-40 hydrogenated castor oil, disodium EDTA and phenoxyethanol;
cooling to 40 ℃ to obtain the disinfectant.
Example 11
The embodiment of the invention provides a household daily disinfectant and a preparation method thereof, wherein the disinfectant comprises the following components in parts by weight:
the preparation method of fructus forsythiae extract and flos Lonicerae extract is the same as that of example 3.
The preparation method of the disinfectant comprises the following steps:
adding propylene glycol and glycerol into water, heating to 85 ℃, and stirring to completely dissolve;
cooling to 52 ℃, adding didecyl dimethyl ammonium chloride, poly aminopropyl biguanide and benzalkonium chloride, and stirring and dispersing uniformly;
cooling to 47 deg.C, adding fructus forsythiae extractive solution and flos Lonicerae extractive solution, citric acid, essence, PEG-40 hydrogenated castor oil, EDTA disodium and phenoxyethanol;
cooling to 40 ℃ to obtain the disinfectant.
Example 12
The embodiment of the invention provides a household daily disinfectant and a preparation method thereof, wherein the disinfectant comprises the following components in parts by weight:
the preparation method of fructus forsythiae extract and flos Lonicerae extract is the same as example 1.
The disinfectant was prepared in the same manner as in example 9.
Example 13
The embodiment of the invention provides a household daily disinfectant and a preparation method thereof, wherein the disinfectant comprises the following components in parts by weight:
the preparation method of fructus forsythiae extract and flos Lonicerae extract is the same as example 1.
The disinfectant was prepared in the same manner as in example 9.
Example 14
The embodiment of the invention provides a household daily disinfectant and a preparation method thereof, wherein the disinfectant comprises the following components in parts by weight:
the preparation method of fructus forsythiae extract and flos Lonicerae extract is the same as example 1.
The disinfectant was prepared in the same manner as in example 9.
Example 15
The embodiment of the invention provides a household daily disinfectant and a preparation method thereof, wherein the disinfectant comprises the following components in parts by weight:
the preparation method of fructus forsythiae extract and flos Lonicerae extract is the same as example 1.
The disinfectant was prepared in the same manner as in example 9.
Example 16
The embodiment of the invention provides a household daily disinfectant and a preparation method thereof, wherein the disinfectant comprises the following components in parts by weight:
the preparation method of fructus forsythiae extract and flos Lonicerae extract is the same as example 1.
The disinfectant was prepared in the same manner as in example 9.
Comparative example 1
This comparative example provides a disinfecting composition having the same ingredients as example 1.
The preparation method of the fructus forsythiae extracting solution comprises the following steps: decocting fructus forsythiae with 10 times of water for 2 times, each for 30 min; filtering, mixing filtrates, and concentrating to 1.25 g/ml;
the preparation method of the honeysuckle extract is the same as that of example 1.
Comparative example 2
This comparative example provides a disinfecting composition having the same ingredients as example 1.
The preparation method of the fructus forsythiae extracting solution comprises the following steps: heating and refluxing fructus forsythiae with 55% v/v ethanol water solution 7 times, and extracting for 30min each time for 2 times; filtering, mixing filtrates, and concentrating to 1.25 g/ml;
the preparation method of the honeysuckle extract is the same as that of example 1.
Comparative example 3
This comparative example provides a disinfecting composition having the same ingredients as example 1.
The preparation method of the fructus forsythiae extracting solution comprises the following steps: ultrasonically extracting fructus forsythiae with 7 times of 20% v/v ethanol water solution for 45min at ultrasonic frequency of 45 Hz; filtering, and concentrating the filtrate to 1.25g/ml to obtain the final product;
the preparation method of the honeysuckle extract is the same as that of example 1.
Comparative example 4
This comparative example provides a disinfecting composition having the same ingredients as example 1.
Wherein the preparation method of fructus forsythiae extractive solution is the same as that of example 1;
the preparation method of the honeysuckle extract comprises the following steps: decocting flos Lonicerae with 10 times of water for 2 times, each for 30 min; filtering, mixing filtrates, and concentrating to 1.25 g/ml.
Comparative example 5
This comparative example provides a disinfecting composition having the same ingredients as example 1.
Wherein the preparation method of fructus forsythiae extractive solution is the same as that of example 1;
the preparation method of the honeysuckle extract comprises the following steps: heating and refluxing flos Lonicerae with 7 times of 57% v/v ethanol water solution for 2 times, each for 30 min; filtering, mixing filtrates, and concentrating to 1.25 g/ml.
Comparative example 6
This comparative example provides a disinfecting composition having the same ingredients as example 1.
Wherein the preparation method of fructus forsythiae extractive solution is the same as that of example 1;
the preparation method of the honeysuckle extract comprises the following steps: ultrasonically extracting flos Lonicerae with 8 times of 55% v/v ethanol water solution for 45min at ultrasonic frequency of 45 Hz; filtering, and concentrating the filtrate to 1.25 g/ml.
Comparative example 7
The comparative example provides a disinfecting composition comprising the following components in parts by weight:
0.1 part of sophora flavescens extract, 0.1 part of honeysuckle extract, 0.1 part of didecyl dimethyl ammonium chloride, 0.4 part of poly-aminopropyl biguanide and 0.1 part of benzalkonium chloride.
Wherein the preparation method of the honeysuckle extract is the same as that of the embodiment 1;
the preparation method of the radix sophorae flavescentis extract comprises the following steps: heating and refluxing the sophora flavescens by 55% v/v ethanol water solution of which the pH is adjusted to 8.2 by sodium hydroxide, wherein the mass of the 55% v/v ethanol water solution is 8 times that of the sophora flavescens; filtering after extraction, concentrating the obtained filtrate, loading on XDA-1 macroporous adsorbent resin, eluting with 90% v/v ethanol water solution, eluting with water, collecting eluate, and concentrating to 1.25 g/ml.
Comparative example 8
The comparative example provides a disinfecting composition comprising the following components in parts by weight:
0.1 part of fructus forsythiae extract, 0.1 part of radix sophorae flavescentis extract, 0.1 part of didecyl dimethyl ammonium chloride, 0.4 part of poly aminopropyl biguanide and 0.1 part of benzalkonium chloride.
Wherein the preparation method of fructus forsythiae extractive solution is the same as example 1, and the preparation method of radix Sophorae Flavescentis extractive solution is the same as comparative example 7.
Comparative example 9
The comparative example provides a disinfecting composition comprising the following components in parts by weight:
0.1 part of didecyl dimethyl ammonium chloride, 0.4 part of polyaminopropyl biguanide and 0.1 part of benzalkonium chloride.
Comparative example 10
The comparative example provides a disinfecting composition comprising the following components in parts by weight:
0.45 part of polyaminopropyl biguanide and 0.15 part of benzalkonium chloride.
Comparative example 11
The comparative example provides a disinfectant and a preparation method thereof, the component proportion and the preparation method of the disinfectant are the same as those of example 9, and the preparation methods of fructus forsythiae extract and honeysuckle extract are the same as those of comparative example 1.
Comparative example 12
The comparative example provides a disinfectant and a preparation method thereof, the component proportion and the preparation method of the disinfectant are the same as those of example 9, and the preparation methods of fructus forsythiae extract and honeysuckle extract are the same as those of comparative example 2.
Comparative example 13
The comparative example provides a disinfectant and a preparation method thereof, the component proportion and the preparation method of the disinfectant are the same as those of example 9, and the preparation methods of fructus forsythiae extract and honeysuckle extract are the same as those of comparative example 3.
Comparative example 14
The comparative example provides a disinfectant and a preparation method thereof, the component proportion and the preparation method of the disinfectant are the same as those of example 9, and the preparation methods of fructus forsythiae extract and honeysuckle extract are the same as those of comparative example 4.
Comparative example 15
The comparative example provides a disinfectant and a preparation method thereof, the component proportion and the preparation method of the disinfectant are the same as those of example 9, and the preparation methods of fructus forsythiae extract and honeysuckle extract are the same as those of comparative example 5.
Comparative example 16
The comparative example provides a disinfectant and a preparation method thereof, the component proportion and the preparation method of the disinfectant are the same as those of example 9, and the preparation methods of fructus forsythiae extract and honeysuckle extract are the same as those of comparative example 6.
Comparative example 17
The comparative example provides a disinfectant and a preparation method thereof, the component proportion of the disinfectant is based on example 9, forsythia extract is replaced by sophora flavescens extract, and the rest components are unchanged, and the preparation method is the same as example 9. The preparation method of radix Sophorae Flavescentis extractive solution and flos Lonicerae extractive solution is the same as that of comparative example 7.
Comparative example 18
The comparative example provides a disinfectant and a preparation method thereof, the component proportion of the disinfectant is based on example 9, honeysuckle extract is replaced by sophora flavescens extract, and the rest components are unchanged, and the preparation method is the same as example 9. The preparation method of radix Sophorae Flavescentis extract and fructus forsythiae extract is the same as that of comparative example 8.
Comparative example 19
The comparative example provides a disinfectant and a preparation method thereof, the component proportion of the disinfectant is based on example 9, forsythia extract and honeysuckle extract are removed, and the other components are unchanged, and the preparation method is the same as example 9.
Comparative example 20
The comparative example provides a disinfectant and a preparation method thereof, the component proportion of the disinfectant is based on example 9, the forsythia extract, the honeysuckle extract and the didecyldimethyl ammonium chloride are removed, the dosage of the polyaminopropyl biguanide is changed to 0.45 part, the dosage of the benzalkonium chloride is changed to 0.15 part, and the other components are not changed, and the preparation method is the same as example 9.
Test example 1
In the test example, the sterilizing effect of the sterilizing compositions prepared in the above examples 1 to 8 and comparative examples 1 to 10 was examined.
The nutrient agar culture medium adopted in the following experiments comprises the following components in proportion: 10g of peptone, 3g of beef extract, 5g of sodium chloride, 17g of agar and 1000mL of distilled water;
the composition of the Sabouraud's medium is: 10g of peptone, 20g of agar, 40g of glucose and 1L of distilled water.
Test strains: staphylococcus aureus (ATCC6538), Escherichia coli (8099), Candida albicans (ATCC 10231).
1. Experimental methods
Washing lawn of 5 th generation of test bacteria in nutrient agar culture medium slant culture (24h) with 5mL of 0.03mol/L Phosphate Buffer Solution (PBS), diluting with the PBS to obtain 5 × 104cfu/ml bacterial suspension.
Using the samples of the disinfecting compositions prepared in examples 1 to 8 and comparative examples 1 to 10 as test sample solutions, 4 tubes of each of the test sample solutions (5mL) and control sample solutions (which were identical in composition to the test sample solutions, but did not contain a forsythia suspensa extract, a honeysuckle extract, didecyldimethylammonium chloride, polyaminopropylbiguanide, or benzalkonium chloride, and were sterilized) were taken.
Respectively dripping 100 mu L of the bacterial suspension into each sample solution to be tested and each control sample solution, uniformly mixing, starting timing, acting for 2min, 5min, 10min, 20min, 1h and 2h, respectively putting the mixed sample solution (0.5mL) into a test tube containing 5mL of PBS by using sterile forceps, fully and uniformly mixing, respectively sucking 0.5mL, placing the sample solution into two plates, pouring 15mL of nutrient agar medium (bacteria) or Sabouraud's agar medium (yeast) cooled to 40-50 ℃, rotating the plates to fully and uniformly stir the plates, and culturing the plates for 48h (bacteria) or 72h (yeast) at 35 +/-2 ℃ after agar is solidified to count viable bacterial colonies.
The experiment was repeated 3 times and the inhibition was calculated as follows:
X＝(A-B)/A×100％；
in the formula: x is the bacteriostasis rate, A is the average colony number of the control sample liquid; b is the average colony number of the sample liquid.
2. And (5) experimental results.
The average bacteriostasis rate of each disinfection composition under different action time is recorded, and the results are shown in tables 1-3.
TABLE 1 bacteriostatic data on Staphylococcus aureus
TABLE 2 bacteriostatic data on E.coli
TABLE 3 bacteriostatic data on Candida albicans
As can be seen from the test results in tables 1 to 3, the sterilization compositions obtained in examples 1 to 3 of the present invention have a bacteriostasis rate of more than 99.9% for 5min to 2h against Staphylococcus aureus, Escherichia coli and Candida albicans, the sterilization compositions obtained in examples 4 to 8 also have a better bacteriostasis effect against Staphylococcus aureus, Escherichia coli and Candida albicans, and the bacteriostasis rates of the sterilization compositions obtained in examples 1 to 8 are better than those of the sterilization compositions obtained in each proportion.
Test example 2
The irritation of the disinfectant solutions prepared in examples 9 to 16 and comparative examples 11 to 20 was examined in this test example.
1. Experimental methods
54 new zealand rabbits with intact skins are used, females are used, the weight of the females is 2.0-2.5 kg, the rabbits are fed by a common-grade rabbit maintaining feed, the temperature is 20-26 ℃, and the relative humidity is 40-70%.
The disinfectant solutions prepared in examples 9 to 16 and in comparative examples 11 to 20 were each subjected to a complete skin irritation test according to the technical specification for disinfection (2002 edition) 2.3.3.3.1 (3 rabbits were used for each sample): about 24 hours before the experiment, removing hairs on two sides of the spinal column of the white rabbit, not damaging the skin, removing hairs in the left side and the right side of the skin by about 3cm multiplied by 3cm respectively, taking 0.5ml of the test solution to be coated on the skin, then covering the skin with two layers of gauze and a layer of cellophane, fixing the skin with adhesive plaster, coating 0.5ml of distilled water on the other side of the skin to be used as a blank control, applying the blank for 4 hours, washing the blank with warm water for 1min to remove the test object, observing local reactions of the skin after 1 hour, 24 hours and 48 hours of removing the test object respectively, and grading the skin irritation reactions according to a table 4. The results are shown in tables 5 and 6.
TABLE 4 skin irritation response Scoring criteria
Table 5 test results of the disinfectant solutions obtained in examples 9 to 16
Table 6 test results of the disinfectant solutions obtained in comparative examples 11 to 20
As can be seen from the results in tables 5 and 6, the disinfectant solutions obtained in examples 9 to 16 are non-irritant to the skin of the rabbits, and the disinfectant solutions obtained in comparative examples 11 to 20 are irritant to the skin of the rabbits.
Test example 3
In this test example, the corrosion of the disinfectant solutions obtained in examples 9 to 16 on metals was examined.
1. Preparation of metal sheets: respectively making stainless steel sheet (304), aluminum sheet and copper sheet into pieces with surface area of 10 + -0.2 cm2And (3) polishing 80 metal sheets by 120-mesh water sand paper to remove surface oxide layers, soaking in soap water for 10min, washing with tap water, washing with distilled water, and drying.
2. The metal sheets of the materials are randomly divided into 8 groups, 10 sheets of each group are respectively soaked in the disinfectant obtained in the embodiment 9-16, and the R value is measured according to GB10124-88 'Uniform corrosion full-immersion test method in a metal material laboratory' at the temperature of 20-25 ℃. The results are shown in Table 7.
Table 7 results of experiments on metal corrosion of the disinfectants obtained in examples 9-16
As can be seen from the results in Table 7, the disinfecting solutions obtained in examples 9 to 16 of the present invention all showed no corrosion to the above metal sheets.
As can be seen from the results of test examples 1, 2 and 3, the disinfectant compositions provided by the examples of the present invention have excellent disinfectant effects and no corrosion to metals, and are mild and non-irritating to the skin compared to the comparative examples.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents or improvements made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (10)
1. The disinfection composition is characterized by comprising the following components in parts by weight: 0.1-1 part of fructus forsythiae extract, 0.1-1 part of honeysuckle extract, 0.05-0.15 part of didecyl dimethyl ammonium chloride, 0.1-0.5 part of poly aminopropyl biguanide and 0.05-0.5 part of benzalkonium chloride.
2. A disinfecting composition as claimed in claim 1, characterized by comprising the following components in parts by weight: 0.1-0.3 part of fructus forsythiae extract, 0.1-0.3 part of honeysuckle extract, 0.05-0.1 part of didecyl dimethyl ammonium chloride, 0.3-0.5 part of poly aminopropyl biguanide and 0.05-0.15 part of benzalkonium chloride.
3. A disinfecting composition as claimed in claim 2, characterized by comprising the following components in parts by weight: 0.1 part of fructus forsythiae extract, 0.1 part of honeysuckle extract, 0.1 part of didecyl dimethyl ammonium chloride, 0.4 part of poly aminopropyl biguanide and 0.1 part of benzalkonium chloride.
4. A disinfecting composition as claimed in any one of claims 1 to 3, characterized in that the fructus forsythiae extract is prepared by a method comprising:
heating and refluxing fructus forsythiae by using 45-65% v/v ethanol water solution with the pH value adjusted to 8-8.5 by using sodium hydroxide; filtering after extraction is finished, concentrating the obtained filtrate, applying polar macroporous adsorption resin to the filtrate, eluting with 85-95% v/v ethanol water solution, eluting with water, collecting the elution fraction, and concentrating to 1.2-1.3 g/ml to obtain the fructus forsythiae extracting solution; wherein the mass of the 45-65% v/v ethanol water solution is 5-8 times of that of the fructus forsythiae.
5. A disinfecting composition as claimed in any one of claims 1 to 3, characterized in that the honeysuckle extract is prepared by a method comprising:
and (2) heating and refluxing the honeysuckle by using 55-60% v/v ethanol water solution with the pH value adjusted to 8-8.5 by using sodium hydroxide, filtering, heating and refluxing the obtained filter residue by using 25-35% v/v ethanol water solution, filtering, combining filtrates, and concentrating to 1.2-1.3 g/ml to obtain the honeysuckle extracting solution.
6. Use of a disinfecting composition according to any one of claims 1 to 5 for the preparation of a household disinfecting product for daily use.
7. A disinfecting solution comprising the disinfecting composition according to any one of claims 1 to 5.
8. The disinfectant liquid according to claim 7, which comprises the following components in parts by weight:
9. the disinfecting solution of claim 8, wherein the humectant is selected from at least one of propylene glycol, glycerin, and butylene glycol; and/or
The preservative is phenoxyethanol; and/or
The solubilizer is PEG-40 hydrogenated castor oil; and/or
The antioxidant is ethylene diamine tetraacetic acid disodium salt.
10. The method for producing the disinfecting solution of claim 8 or 9, comprising the steps of:
adding the humectant into the water, and stirring until the humectant is dissolved;
adding the didecyl dimethyl ammonium chloride, the polyaminopropyl biguanide and the benzalkonium chloride, and stirring and dispersing uniformly;
adding the fructus forsythiae extract, the honeysuckle extract, the citric acid, the essence, the solubilizer, the antioxidant and the preservative, and uniformly mixing to obtain the disinfectant.
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| CN114600884A (en) * | 2022-05-13 | 2022-06-10 | 广东洛斯特制药有限公司 | Benzalkonium chloride composite sterilization and disinfection spray and preparation method thereof |
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