CN114045217B - Air microorganism sampling device and air microorganism sampling and detecting integrated device - Google Patents
Air microorganism sampling device and air microorganism sampling and detecting integrated device Download PDFInfo
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- CN114045217B CN114045217B CN202210023879.6A CN202210023879A CN114045217B CN 114045217 B CN114045217 B CN 114045217B CN 202210023879 A CN202210023879 A CN 202210023879A CN 114045217 B CN114045217 B CN 114045217B
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Abstract
The invention discloses an air microorganism sampling device, which relates to the technical field of microorganism detection and comprises the following components: the sampling device comprises a sampling pipe and a filter membrane, wherein the filter membrane is arranged in the sampling pipe, one end of the sampling pipe is provided with an air extractor for extracting gas in the sampling pipe, the other end of the sampling pipe is used for introducing air, and the filter membrane is used for intercepting microorganisms in the air flowing through the filter membrane on the filter membrane; the invention also provides an air microorganism sampling and detecting integrated device, which comprises the air microorganism sampling device, a nucleic acid detecting device and a nucleic acid extracting device, wherein the nucleic acid extracting device is used for extracting nucleic acid from a microorganism sample collected by the air microorganism sampling device; the air microorganism sampling device and the air microorganism sampling and detecting integrated device provided by the invention improve the sampling efficiency and the detection efficiency.
Description
Technical Field
The invention relates to the technical field of microorganism detection, in particular to an air microorganism sampling device and an air microorganism sampling and detecting integrated device.
Background
At present, methods for detecting air microorganisms mainly include a filtration method, an impact method, a sedimentation method and the like. The filtration method is to pass air through a liquid adsorbent, and detect airborne microorganisms by culturing the adsorbent; the impact method is that air microorganisms are impacted and adsorbed onto a culture medium at a certain flow rate by using a suction fan or a vacuum pump, and detection is carried out through microorganism culture; the sedimentation method is that a culture medium plate is directly placed in the air and exposed for a certain time, and then culture is carried out, so that microorganism detection is carried out, the method is generally used, but only particle bacteria with certain size can be reduced onto the culture medium within a certain time, so that the detected bacteria content is not accurate enough, and the detection result is less than the actual bacteria amount; the speed of collecting microorganisms by the above commonly used filtration method, impact method and sedimentation method is slow, and the microorganisms are required to be cultured to a required amount after collection so that the microorganisms can be put into detection, which seriously affects the detection progress, so that a novel scheme is urgently needed to solve the above problems.
Disclosure of Invention
The invention aims to provide an air microorganism sampling device and an air microorganism sampling and detecting integrated device, which are used for solving the problems in the prior art and improving the sampling efficiency.
In order to achieve the purpose, the invention provides the following scheme:
the invention provides an air microorganism sampling device, comprising: sampling pipe and filter membrane, be provided with in the sampling pipe the filter membrane, the one end of sampling pipe is provided with and is used for the extraction the gaseous air exhaust device in sampling pipe, and the other end is used for admitting air, the filter membrane is used for will flowing through microorganism in the air of filter membrane is held back on the filter membrane.
Preferably, a plurality of the filter membranes are provided, the pore sizes of the filter membranes are different, the filter membranes are sequentially arranged in the sampling tube at intervals along the gas flowing direction of the sampling tube, and the pore sizes of the filter membranes are gradually reduced along the gas flowing direction.
Preferably, still include at least one filter membrane unwinding device, the top of sampling pipe is provided with the filter membrane import, the bottom of sampling pipe is provided with the filter membrane export, filter membrane unwinding device can be right the filter membrane unreels, the filter membrane passes through the filter membrane import gets into in the sampling pipe, in the sampling pipe the filter membrane just after having gathered the microorganism can be followed under filter membrane unwinding device's the effect of unreeling the filter membrane export comes out, one the filter membrane unwinding device corresponds one the filter membrane.
Preferably, filter membrane unwinding device includes top drive gyro wheel and bottom drive gyro wheel, filter membrane unwinding device's the action of unreeling realizes through top drive gyro wheel and bottom drive gyro wheel, top drive gyro wheel includes two top gyro wheels, two top gyro wheel all set up in filter membrane import department just can press from both sides tightly the filter membrane, two can drive during the top gyro wheel rotates the filter membrane to the filter membrane export motion, bottom drive gyro wheel includes two end roller groups, two end roller group all sets up the filter membrane exit, and two end roller group is located respectively the width direction's of filter membrane both ends, each end roller group all includes two end gyro wheels, and is same two in the end roller group two end gyro wheels homogeneous phase set up in the filter membrane exit the both sides of filter membrane, and it is same in the end roller group two can drive during the rolling of end gyro wheel the filter membrane is towards keeping away from the both sides of sampling pipe And (4) moving in a direction.
Preferably, still include a plurality of leak protection devices, one leak protection device corresponds to one the filter membrane sets up, each leak protection device all includes two leak protection spares, two the leak protection spare all fixed set up in just be located respectively in the sampling tube the width direction's of filter membrane both ends, the leak protection piece orientation the one end of filter membrane is provided with an edge the draw-in groove that the filter membrane direction of motion extends, two the leak protection spare deviates from the one end of filter membrane all with the sampling tube inner wall in close contact with, in the sampling tube the both ends border of filter membrane width direction is located two respectively in the draw-in groove.
The invention also provides an air microorganism sampling and detecting integrated device, which comprises the air microorganism sampling device;
the nucleic acid extraction device is used for extracting nucleic acid from the microorganism sample collected by the air microorganism sampling device;
a nucleic acid detection device for detecting nucleic acid from the nucleic acid extracted by the nucleic acid extraction device.
Preferably, the nucleic acid extraction device comprises a lysate storage container and magnetic beads for adsorbing nucleic acids, the lysate storage container is used for containing lysate and the magnetic beads, and the lysate in the lysate storage container can be used for cracking microorganisms on the filter membrane.
Preferably, the device further comprises a crushing device, the crushing device is arranged below the filter membrane outlet on the sampling pipe, and the filter membrane can enter the crushing device after coming out of the filter membrane outlet and fall into the lysate storage container after being crushed.
Preferably, the nucleic acid extraction device further comprises a waste liquid storage container, an eluent storage container and a washing liquid storage container, wherein the waste liquid storage container is used for containing waste liquid, the washing liquid storage container is used for containing nucleic acid washing liquid, the eluent storage container is used for storing eluent, the lysis liquid storage container is communicated with the waste liquid storage container through a first pipeline, the lysis liquid storage container is communicated with the washing liquid storage container through a second pipeline, the eluent storage container is communicated with the lysis liquid storage container through a third pipeline, pumps are arranged on the second pipeline and the third pipeline, and the first pipeline is communicated with the bottom of the lysis liquid storage container.
Preferably, the nucleic acid detection device comprises a detection box, a microfluidic chip for detecting nucleic acid, a temperature control module, an experimental result information detection module and a controller, wherein the microfluidic chip and the temperature control module are arranged in the detection box, the microfluidic chip is arranged on a detection platform in the detection box, the temperature control module is used for heating the detection platform to adjust the temperature in the microfluidic chip, and the experimental result information detection module is used for recording the detection result on the microfluidic chip and transmitting the detection result to the controller; the nucleic acid extraction device and the nucleic acid detection device are both provided with a plurality of devices, and one nucleic acid extraction device and one nucleic acid detection device are both arranged corresponding to one filter membrane.
Compared with the prior art, the invention has the following technical effects:
the air microorganism sampling device and the air microorganism sampling and detecting integrated device provided by the invention adopt the air extractor to accelerate the air flow speed in the sampling pipe, so that the filter membrane can filter a large amount of air in a short time, and the microorganism sampling efficiency is improved.
Furthermore, the device realizes the graded sampling of the air microorganisms by setting the filter membranes with different apertures, and the different air microorganisms are graded and collected, so that the device is more favorable for the detection of the air microorganisms.
Furthermore, the structural style of the filter membrane unreeling device is convenient for workers to replace the filter membrane in the sampling pipe.
Furthermore, through the design to each part and pipeline, this device collects sample collection, nucleic acid extraction and detects in an organic whole, and is easy and simple to handle, has saved time cost and human cost.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments will be briefly described below.
FIG. 1 is a schematic structural diagram of an integrated air microorganism sampling and detecting device according to a second embodiment;
FIG. 2 is a schematic view of the air microorganism sampling apparatus and the pulverizing apparatus shown in FIG. 1;
in the figure: 1-sampling tube, 2-filter membrane, 5-air extractor, 6-pump, 7-lysate storage container, 8-magnetic bead, 9-valve, 10-washing liquid storage container, 11-waste liquid storage container, 12-eluent storage container, 13-data processing module, 14-temperature control module, 15-experimental result information detection module, 16-detection box, 17-microfluidic chip, 18-data output device, 19-filter membrane storage cavity, 20-pulverizer, 21-filter membrane reel, 22-reel shaft, 23-top drive roller, 25-anti-leakage piece, 28-bottom drive roller.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention aims to provide an air microorganism sampling device and an air microorganism sampling and detecting integrated device, which are used for solving the problems in the prior art and improving the sampling efficiency.
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.
Example one
The embodiment provides an air microorganism sampling device, as shown in fig. 1-2, including: the sampling device comprises a sampling pipe 1 and a filter membrane 2, wherein the filter membrane 2 is arranged in the sampling pipe 1, one end of the sampling pipe 1 is provided with an air extractor 5 for extracting gas in the sampling pipe 1, the other end of the sampling pipe is used for air inlet, and the filter membrane 2 is used for intercepting microorganisms in the air flowing through the filter membrane 2 on the filter membrane 2; adopt air exhaust device 5 to accelerate the air flow speed in sampling pipe 1 for filter membrane 2 can filter a large amount of air in the short time, has improved microorganism sampling efficiency.
Further, be provided with a plurality of filter membranes 2 in the sampling pipe 1, the aperture size on each filter membrane 2 is all different, a plurality of filter membranes 2 along the direction of sampling pipe 1 gas flow and possess in proper order and set up in sampling pipe 1 at intervals, the aperture of each filter membrane 2 diminishes along the direction of gas flow gradually to carry out solitary sampling to the microorganism of different particle diameters, specific setting is three-layer filter membrane 2, wherein the microorganism (mainly for the fungus) that the diameter is greater than 7 μm is held back to first order filter membrane 2, the microorganism (mainly for bacterium and the fungi that the diameter is less than) of diameter 0.5-7 μm is held back to second order filter membrane 2, the microorganism (like the virus) that is less than 0.5 μm is held back to tertiary filter membrane 2.
Further, air microorganism sampling device still includes at least one filter membrane unwinding device, the top of sampling pipe 1 is provided with the filter membrane import, the bottom of sampling pipe 1 is provided with the filter membrane export, filter membrane unwinding device can unreel filter membrane 2, filter membrane 2 passes through in the filter membrane import gets into sampling pipe 1, filter membrane 2 in the sampling pipe 1 just can follow the filter membrane export under filter membrane unwinding device's the effect of unreeling after having gathered the microorganism and come out, a filter membrane unwinding device corresponds a filter membrane 2, set up filter membrane unwinding device so that change filter membrane 2 in the sampling pipe 1.
Further, the filter membrane unwinding device comprises a top driving roller 23 and a bottom driving roller 28, the unwinding action of the filter membrane unwinding device is realized by the top driving roller 23 and the bottom driving roller 28, the top driving roller 23 comprises two top rollers, the two top rollers are both arranged at the inlet of the filter membrane and can clamp the filter membrane 2, the two top rollers can drive the filter membrane 2 to move towards the outlet of the filter membrane when rotating, the bottom driving roller 28 comprises two bottom roller sets, the two bottom roller sets are both provided with the outlet of the filter membrane, and are respectively arranged at the two ends of the width direction of the filter membrane 2 to prevent the bottom rollers from rolling and damaging the microorganisms on the filter membrane 2, each bottom roller set comprises two bottom rollers, the two bottom rollers in the same bottom roller set are opposite to the two sides of the filter membrane 2 arranged at the outlet of the filter membrane, and the two bottom rollers in the same bottom roller set can drive the filter membrane 2 to move towards the direction far away from the sampling tube 1 when rolling, the top driving roller 23 and the bottom driving roller 28 are matched and matched to realize the unreeling process of the filter membrane 2, and the top driving roller 23 and the bottom driving roller 28 are respectively driven by a motor to rotate.
Further, the filter film unwinding device comprises a filter film storage chamber 19 for accommodating the filter film roll 21, and a roll shaft 22 for supporting the filter film roll 21.
Furthermore, the air microorganism sampling device also comprises a plurality of leak-proof devices, one leak-proof device is arranged corresponding to one filter membrane, each leak-proof device comprises two leak-proof parts 25, the two leak-proof parts 25 are fixedly arranged in the sampling tube 1 and respectively positioned at two ends of the width direction of the filter membrane 2, one end of the leak-proof part 25 facing the filter membrane 2 is provided with a clamping groove extending along the movement direction of the filter membrane 2, one ends of the two leak-proof parts 25 departing from the filter membrane 2 are tightly contacted with the inner wall of the sampling tube 1, two ends of the width direction of the filter membrane 2 in the sampling tube 1 are respectively positioned in the two clamping grooves, each leak-proof part 25 is composed of two baffle plates vertically arranged in the sampling tube 1, a gap between the two baffle plates is the clamping groove, the four baffle plates are lightly contacted with the filter membrane 2 and can not clamp the filter membrane 2, thereby most of the air microorganisms can be intercepted on the filter membrane 2 during sampling, without passing directly through the gap between the filter membrane 2 and the sampling tube 1.
Example two
The embodiment provides an air microorganism sampling and detecting integrated device, which comprises the air microorganism sampling device in the first embodiment;
the nucleic acid extraction device is used for extracting nucleic acid from the microorganism sample collected by the air microorganism sampling device;
and the nucleic acid detection device is used for detecting the nucleic acid extracted by the nucleic acid extraction device.
Further, the nucleic acid extraction device comprises a lysis solution storage container 7 and magnetic beads 8 for adsorbing nucleic acid, wherein the lysis solution storage container 7 is used for containing lysis solution and the magnetic beads 8, the lysis solution in the lysis solution storage container 7 can be used for lysing the microorganisms on the filter membrane 2, the lysis solution is used for performing a lysis process on the microorganisms after the filter membrane 2 carrying the microorganisms is placed in the lysis solution, and the nucleic acid of the lysed microorganisms is adsorbed by the magnetic beads 8.
Further, integrative device of air microorganism sampling detection still includes reducing mechanism 20, and reducing mechanism 20 sets up the below of the 2 exports of filter membrane on sampling pipe 1, and filter membrane 2 can enter into reducing mechanism 20 after coming out from the filter membrane export, falls to lysate storage container 7 after smashing, and the filter membrane 2 after smashing distributes more evenly in the lysate, can improve the schizolysis efficiency.
Further, the nucleic acid extraction device further comprises a waste liquid storage container 11, an eluent storage container 12 and a washing liquid storage container 10, the waste liquid storage container 11 is used for containing waste liquid, the washing liquid storage container 10 is used for containing nucleic acid washing liquid, the eluent storage container 12 is used for storing eluent, the cracking liquid storage container 7 is communicated with the waste liquid storage container 11 through a first pipeline, the cracking liquid storage container 7 is communicated with the washing liquid storage container 10 through a second pipeline, the eluent storage container 12 is communicated with the cracking liquid storage container 7 through a third pipeline, pumps 6 are arranged on the second pipeline and the third pipeline, the first pipeline is communicated with the bottom of the cracking liquid storage container 7, the first pipeline is used for discharging cracking liquid of microorganisms cracked in the cracking liquid storage container 7 into the waste liquid storage container 11 for collection, the second pipeline is used for introducing the washing liquid in the washing liquid storage container 10 into the cracking liquid storage container 7 for washing sundries on magnetic beads 8 in the cracking liquid storage container The third pipeline is used for letting in the eluent storage container 12 to the nucleic acid on the magnetic bead 8 of the lysis solution storage container 7 for elution, the bottom of the lysis solution storage container 7 is provided with a filter screen, the magnetic bead 8 is arranged above the filter screen, and the magnetic bead 8 is prevented from being discharged to the waste liquid storage container 11 through the first pipeline.
Further, the nucleic acid detection device comprises a detection box 16, a micro-fluidic chip 17 for detecting nucleic acid, a temperature control module 14, an experiment result information detection module 15 and a controller, wherein the micro-fluidic chip 17 and the temperature control module 14 are arranged in the detection box 16, the detection box 16 is communicated with a lysate storage container 7 through a fourth pipeline, a pump 6 is arranged on the fourth pipeline, the micro-fluidic chip 17 is placed on a detection platform in the detection box 16, the temperature control module 14 is used for heating the detection platform to adjust the temperature in the micro-fluidic chip 17, the experiment result information detection module 15 is used for recording and transmitting the detection result on the micro-fluidic chip 17 to the controller, and the controller can process and display an experiment structure; the micro-fluidic chip 17 carries out amplification and color development reaction on nucleic acid, the experimental result information detection module 15 can be a camera or a fluorescence detection module, a plurality of filter membranes 2 are arranged in the sampling tube 1, the sizes of the apertures on the filter membranes 2 are different, the filter membranes 2 are arranged in the sampling tube 1 in sequence along the gas flowing direction of the sampling tube 1 at intervals, and the apertures of the filter membranes 2 are gradually reduced along the gas flowing direction; the nucleic acid extraction device and the nucleic acid detection device are both provided with a plurality of devices, and one nucleic acid extraction device and one nucleic acid detection device are both arranged corresponding to one filter membrane 2.
Further, the controller includes a data processing module 13 and a data output device 18, and the data output device 18 is preferably a computer.
The specific using process is as follows:
the air extraction device 5 starts to suck air, the air enters the sampling tube 1 from one end of the sampling tube 1, microorganisms with different particle sizes can be respectively intercepted on filter membranes 2 with three pore sizes, after sampling is completed, the filter membranes 2 pass through the crushing device 20 and are cut into fragments by the crushing device 20, the fragments enter a lysate storage container 7, preferably, a stirring blade for stirring the fragments and the lysate can be further arranged in the lysate storage container 7 to prevent the fragments from floating on the surface layer of the lysate, microbial nucleic acid is released into the lysate and is adsorbed onto magnetic beads 8 under the cracking action of the lysate, a valve 9 is arranged on a first pipeline, and the valve 9 is opened to enable the lysate to flow out of the lysate storage container 7 and enter a waste liquid storage container 11; the pump 6 on the second pipeline is opened, the washing liquid in the washing liquid storage container 10 is pumped into the lysis liquid storage container 7, and the magnetic beads 8 in the container are washed; opening a valve 9 to enable the washing liquid to flow out of the cracking liquid storage container 7 and enter a waste liquid storage container 11; opening the pump 6 on the third pipeline, pumping the eluent in the eluent storage container 12 into the lysate storage container 7, and eluting the nucleic acid on the magnetic beads 8; opening a pump 6 on the fourth pipeline to pump the eluent in the lysate storage container 7 into a detection box 16 (the pipeline corresponding to the operation is provided with a filter screen at the head part of one end extending into the lysate storage container 7, so that magnetic beads 8 can be prevented from being pumped into the pipeline), and detecting the microbial sample; the detection box 16 is internally provided with a micro-fluidic chip 17, a temperature control module 14 and a camera/fluorescence detection module, the micro-fluidic chip 17 contains microorganism detection reagents, the temperature control module 14 controls the temperature to enable the detection reaction to be normally carried out, the camera/fluorescence detection module can transmit the detection result to the data processing module 13 through photographing/fluorescence detection, the data processing module 13 is connected with the data output device 18 (computer), the data processing module 13 can transmit the result to the data output device 18 (computer) after processing the detection data, and the air microorganism detection result can be visually checked through the data output device 18 (computer).
The principle and the implementation mode of the invention are explained by applying a specific example, and the description of the embodiment is only used for helping to understand the method and the core idea of the invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, the specific embodiments and the application range may be changed. In view of the above, the present disclosure should not be construed as limiting the invention.
Claims (7)
1. An air microorganism sampling device, characterized in that: the method comprises the following steps: the sampling device comprises a sampling pipe and a filter membrane, wherein the filter membrane is arranged in the sampling pipe, one end of the sampling pipe is provided with an air exhaust device for exhausting gas in the sampling pipe, the other end of the sampling pipe is used for air intake, and the filter membrane is used for retaining microorganisms in air flowing through the filter membrane on the filter membrane; the filter membranes are arranged in the sampling pipe in sequence along the gas flowing direction of the sampling pipe at intervals, and the pore diameters of the filter membranes gradually decrease along the gas flowing direction; the sampling device comprises a sampling pipe, and is characterized by further comprising at least one filter membrane unreeling device, wherein a filter membrane inlet is formed in the top of the sampling pipe, a filter membrane outlet is formed in the bottom of the sampling pipe, the filter membrane unreeling device can unreel the filter membrane, the filter membrane enters the sampling pipe through the filter membrane inlet, the filter membrane in the sampling pipe can come out of the filter membrane outlet after the collection of microorganisms and under the unreeling effect of the filter membrane unreeling device, and one filter membrane unreeling device corresponds to one filter membrane; the filter membrane unwinding device comprises a top driving roller and a bottom driving roller, unwinding action of the filter membrane unwinding device is realized through the top driving roller and the bottom driving roller, the top driving roller comprises two top rollers, the two top rollers are arranged at the filter membrane inlet and can clamp the two sides of the filter membrane, the two top rollers can drive the filter membrane to move towards the filter membrane outlet when rotating, the bottom driving roller comprises two bottom roller groups, the two bottom roller groups are arranged at the filter membrane outlet and are respectively positioned at the two ends of the filter membrane in the width direction, each bottom roller group comprises two bottom rollers, and the two bottom rollers in the same bottom roller group are respectively arranged at the two sides of the filter membrane outlet and can drive the filter membrane to move away from the filter membrane when rolling And (5) sampling the direction movement of the tube.
2. The air microbe sampling device of claim 1, wherein: still include a plurality of leak protection devices, one leak protection device corresponds to one the filter membrane sets up, each leak protection device all includes two leak protection pieces, two leak protection pieces all fixed set up in just be located respectively in the sampling tube the width direction's of filter membrane both ends, leak protection piece orientation the one end of filter membrane is provided with an edge the draw-in groove that the filter membrane direction of motion extends, leak protection piece deviates from the one end of filter membrane all with the sampling tube inner wall in close contact with, in the sampling tube the both ends border of filter membrane width direction is located two respectively in the draw-in groove.
3. The utility model provides an integrative device of air microorganism sampling detection which characterized in that: comprising the air microorganism sampling device of any one of claims 1-2;
the nucleic acid extraction device is used for extracting nucleic acid from the microorganism sample collected by the air microorganism sampling device;
a nucleic acid detection device for detecting nucleic acid from the nucleic acid extracted by the nucleic acid extraction device.
4. The air microorganism sampling and detecting integrated device according to claim 3, characterized in that: the nucleic acid extraction device comprises a lysate storage container and magnetic beads for adsorbing nucleic acids, wherein the lysate storage container is used for containing lysate and the magnetic beads, and the lysate in the lysate storage container can be used for cracking microorganisms on the filter membrane.
5. The air microorganism sampling and detecting integrated device according to claim 4, characterized in that: still include reducing mechanism, reducing mechanism set up in the below of the filter membrane export on the sampling pipe, the filter membrane follows the filter membrane export can get into after coming out among the reducing mechanism, fall after smashing to in the lysate storage container.
6. The air microorganism sampling and detecting integrated device according to claim 4, characterized in that: the nucleic acid extraction device further comprises a waste liquid storage container, an eluent storage container and a washing liquid storage container, wherein the waste liquid storage container is used for containing waste liquid, the washing liquid storage container is used for containing nucleic acid washing liquid, the eluent storage container is used for storing eluent, the lysis liquid storage container is communicated with the waste liquid storage container through a first pipeline, the lysis liquid storage container is communicated with the washing liquid storage container through a second pipeline, the eluent storage container is communicated with the lysis liquid storage container through a third pipeline, pumps are arranged on the second pipeline and the third pipeline, and the first pipeline is communicated with the bottom of the lysis liquid storage container.
7. The air microorganism sampling and detecting integrated device according to claim 5, characterized in that: the nucleic acid detection device comprises a detection box, a micro-fluidic chip for detecting nucleic acid, a temperature control module, an experiment result information detection module and a controller, wherein the detection box is communicated with the lysate storage container through a fourth pipeline, a pump is arranged on the fourth pipeline, the micro-fluidic chip and the temperature control module are arranged in the detection box at the same time, the micro-fluidic chip is placed on a detection platform in the detection box, the temperature control module is used for heating the detection platform to further adjust the temperature in the micro-fluidic chip, the experiment result information detection module is used for recording the detection result on the micro-fluidic chip and transmitting the detection result to the controller, and the controller can process and display the experiment result;
the nucleic acid extraction device and the nucleic acid detection device are both provided with a plurality of devices, and one nucleic acid extraction device and one nucleic acid detection device are both arranged corresponding to one filter membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210023879.6A CN114045217B (en) | 2022-01-11 | 2022-01-11 | Air microorganism sampling device and air microorganism sampling and detecting integrated device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202210023879.6A CN114045217B (en) | 2022-01-11 | 2022-01-11 | Air microorganism sampling device and air microorganism sampling and detecting integrated device |
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| CN114045217A (en) | 2022-02-15 |
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