CN114045164A - Preparation method of surface-enhanced Raman spectrum probe and product thereof - Google Patents

Preparation method of surface-enhanced Raman spectrum probe and product thereof Download PDF

Info

Publication number
CN114045164A
CN114045164A CN202111308137.XA CN202111308137A CN114045164A CN 114045164 A CN114045164 A CN 114045164A CN 202111308137 A CN202111308137 A CN 202111308137A CN 114045164 A CN114045164 A CN 114045164A
Authority
CN
China
Prior art keywords
enhanced raman
gold
raman spectroscopy
gns
probe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202111308137.XA
Other languages
Chinese (zh)
Inventor
崔大祥
陈晓敏
张禹娜
章阿敏
徐艳
梁辉
崔明青
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanghai National Engineering Research Center for Nanotechnology Co Ltd
Original Assignee
Shanghai National Engineering Research Center for Nanotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shanghai National Engineering Research Center for Nanotechnology Co Ltd filed Critical Shanghai National Engineering Research Center for Nanotechnology Co Ltd
Priority to CN202111308137.XA priority Critical patent/CN114045164A/en
Publication of CN114045164A publication Critical patent/CN114045164A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
    • C09K11/02—Use of particular materials as binders, particle coatings or suspension media therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y20/00—Nanooptics, e.g. quantum optics or photonic crystals
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y40/00—Manufacture or treatment of nanostructures
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
    • C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
    • C09K11/58—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials containing copper, silver or gold
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Nanotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Materials Engineering (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Optics & Photonics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Manufacturing & Machinery (AREA)
  • Composite Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

The invention discloses a preparation method of a surface enhanced Raman spectrum probe and a product thereof, wherein the preparation method comprises the following steps: synthesizing a substrate material with a gold-silver nano composite structure, wherein the gold nano star is used as a core and silver is used as a shell, modifying a reporter molecule and a cysteamine molecule on the surface of the substrate material, and connecting a target recognition molecule to prepare the specific surface enhanced Raman spectroscopy probe. The preparation method of the surface-enhanced Raman spectrum probe has the advantages of simple operation, low cost, good stability, high repeatability, high sensitivity and good biomedical application prospect.

Description

Preparation method of surface-enhanced Raman spectrum probe and product thereof
Technical Field
The invention relates to the technical field of biomedical detection, in particular to a preparation method of a surface enhanced Raman spectrum probe and a product thereof.
Background
Malignant tumor has high mortality, high metastasis rate and high recurrence rate, and is an important disease threatening human health. Data published by the international cancer research institute indicate that there are about 1930 million new cases of cancer and 1000 million cases of cancer death worldwide in 2020. Tumor markers are substances that are present in, produced by, or produced by the interaction of a host with a tumor and that indicate the presence of a tumor. The tumor marker can be used for screening, diagnosing, monitoring treatment, evaluating prognosis and the like of tumor patients. Tumor markers are various in types, and common detection methods include radioimmunoassay, enzyme-linked immunosorbent assay, immunofluorescence assay, chemiluminescence immunoassay, molecular biology method and the like, but the common detection methods have defects of different degrees in aspects of experiment operation, detection conditions, detection cost, sensitivity and the like. At present, the research and development of a tumor marker detection method with high sensitivity, good specificity and simple and rapid operation is still one of important research directions in the field of global biomedical detection, and especially the realization of the detection of trace tumor markers has great significance for early screening of tumors.
The surface-enhanced Raman spectroscopy (SERS) technology is based on the principle of Raman spectroscopy and amplifies vibration information of detection molecules by means of electromagnetic enhancement and chemical enhancement mechanisms of metal nanoparticles (such as gold, silver, copper and the like) so as to obtain stronger signals (stronger than common Raman spectroscopy by 10 degrees)2~1012Multiple) of the spectrum. The surface enhanced Raman spectroscopy technology has the advantages of rapid detection, narrow Raman spectrum peak, high sensitivity (single molecule detection can be realized), good specificity, difficult quenching, no interference of biological sample autofluorescence and water, near infrared excitation, small damage to the biological sample and the like. Surface enhanced raman spectroscopy can be divided into two broad categories, label-free detection and labeled detection. Unmarked surface enhancementThe raman spectroscopy detection can directly detect the target molecules, but the surface enhanced raman spectroscopy effect is limited by the target molecules. Labeled surface-enhanced raman spectroscopy (i.e., surface-enhanced raman spectroscopy probe detection) indirectly detects target molecules by detecting the raman spectrum of reporter molecules, and thus the effect of surface-enhanced raman spectroscopy is not limited by the target molecules themselves, which is also the most commonly adopted strategy in the field of biomedical research in current surface-enhanced raman spectroscopy. However, the tumor marker detection technology based on the surface enhanced raman spectroscopy probe detection is still in the initial stage, and many problems in the preparation and application of the probe still need to be solved.
Disclosure of Invention
In view of this, the present invention aims to provide a method for preparing a surface enhanced raman spectroscopy probe, which has high sensitivity, good stability and simple preparation.
Yet another object of the present invention is to: provides a surface-enhanced Raman spectrum probe product prepared by the method.
The invention aims to provide the following scheme for realization: a preparation method of a surface enhanced Raman spectrum probe comprises the following steps:
step one, synthesizing a gold nano star by a gold seed growth method;
coating a silver shell on the surface of the gold nano star obtained in the step one by an ascorbic acid reduction silver nitrate method to synthesize a surface enhanced Raman spectroscopy substrate material with a gold-silver nano composite structure;
performing ultrasonic reaction on the surface-enhanced Raman spectrum substrate material obtained in the step two and a Raman reporter molecule at the temperature of 30-40 ℃ for 0.5-1 hour to obtain a surface-enhanced Raman spectrum substrate material modified with the reporter molecule;
step four, carrying out ultrasonic reaction on the surface enhanced Raman spectrum substrate material modified with the reporter molecule obtained in the step three and cysteamine molecules at the temperature of 30-40 ℃ for 1-2 hours to obtain nanoparticles which are stable surface enhanced Raman spectrum probes;
and step five, connecting the targeted recognition molecules to the surface of the stable surface-enhanced Raman spectrum probe prepared in the step four through an EDC (EDC) activation reaction of 1-ethyl- (3-dimethylaminopropyl) carbonyldiimine to obtain the specific surface-enhanced Raman spectrum probe.
The invention provides a preparation method which is low in preparation cost and easy to control.
Further, the surface-enhanced Raman spectrum substrate material is gold-silver nano composite particles which take gold nano stars as cores and silver as shells.
In the third step, the Raman reporter molecule is a Raman reporter molecule carrying a sulfydryl (-SH), and comprises 4-aminothiophenol 4-ATP, 4-aminobenzoic acid and the like.
In the fifth step, the target recognition molecule is a molecule which carries or is modified with carboxyl (-COOH) and comprises folic acid FA, an antibody, a nucleic acid aptamer and the like.
(5) And (3) connecting a target recognition molecule (such as folic acid) carrying or modified with carboxyl (-COOH) to the surface of the nanoparticle obtained in the step (4) through 1-ethyl- (3-dimethylaminopropyl) carbodiimide (EDC) activation reaction, thereby obtaining the specific surface enhanced Raman spectroscopy probe.
The invention provides a surface-enhanced Raman spectrum probe prepared according to any one of the methods.
The product takes gold-silver nano composite particles with gold nano star as a core and silver as a shell as a substrate material for signal amplification, a reporter molecule is modified on the surface of the substrate material for signal detection, and the modified cysteamine molecule not only can stabilize the nano particles but also can be connected with a targeted recognition molecule which is connected with the targeted recognition molecule for capturing target molecules.
Compared with the prior art, the invention has the beneficial effects that:
(1) the surface-enhanced Raman spectrum probe adopts gold-silver nano composite structure particles which take gold nano stars as cores and silver as shells as substrate materials, and the gold-silver bimetallic nanoparticles with rough surfaces can obtain more favorable surface plasmon characteristics, so that the Raman spectrum signal intensity of reporter molecules is favorably improved.
(2) The surface-enhanced Raman spectrum probe provided by the invention is simple and safe in preparation method, low in cost, high in repeatability, good in stability, high in sensitivity and good in application prospect.
Drawings
FIG. 1 shows the result of transmission electron microscope detection of a surface enhanced Raman spectroscopy probe GNS @ Ag-ATP;
FIG. 2 shows the UV-visible absorption spectrum detection results of the surface-enhanced Raman spectroscopy probe GNS @ Ag-ATP and the folate-labeled surface-enhanced Raman spectroscopy probe GNS @ Ag-ATP-FA;
FIG. 3 shows the Raman spectrum detection results of the surface-enhanced Raman spectrum probe GNS @ Ag-ATP and the folate-labeled surface-enhanced Raman spectrum probe GNS @ Ag-ATP-FA.
Detailed Description
Preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.
Examples
A surface-enhanced Raman spectrum probe is prepared by the following steps:
step one, synthesizing a gold nano star by a gold seed growth method:
synthesizing a surface-enhanced Raman spectroscopy substrate material GNS @ Ag of a gold-silver nano composite structure with a Gold Nano Star (GNS) as a core and silver as a shell, and specifically comprising the following steps:
(1) preparing gold seeds: 6 mL of a 1% trisodium citrate solution are quickly added to 40 mL of boiling 1mM tetrachloroauric acid (HAuCl)4) Continuously heating and stirring the solution for 15 minutes until the color of the solution becomes wine red, which indicates that the gold seeds are successfully generated;
(2) and (3) synthesizing GNS: mu.L of the gold seeds prepared in (1) above was taken and mixed with 30 mL of 0.1 mM HAuCl containing 300. mu.L of hydrochloric acid (HCl, 1M)4Mixing, and adding 150 μ L silver nitrate (AgNO)36 mM) and 150 μ L ascorbic acid (AA, 0.1M) are quickly mixed, the color of the solution is quickly changed into dark green, and the synthesis of GNS is illustrated; subsequently, 300 μ L of cetyltrimethylammonium bromide (CTAB, 0.1M) was added and mixed well to stabilize GNS; centrifuging at 8000-9000 rpm for 10-15 min at 4 deg.C, removing supernatant, adding 30 mL CTAB (1 mM) for resuspension to obtain Gold Nanostar (GNS) solution, and heating to 4 deg.CAnd (5) storing for later use.
Step two, coating a silver shell on the surface of the GNS synthesized in the step one by an ascorbic acid reduction silver nitrate method to synthesize the surface enhanced Raman spectroscopy substrate material with the gold-silver nano composite structure, and the method comprises the following steps:
to 5 mL of synthetic Gold Nanocene (GNS) solution were added 20. mu.L of ascorbic acid AA (0.1M) and 20. mu.L of silver nitrate (AgNO)30.1M), uniformly mixing, adding 5-10 mu L of ammonia water, rapidly mixing for 5-10 minutes, and obtaining the synthesized GNS @ Ag, wherein the solution is brown in color.
Step three, preparing a Raman reporter molecule 4-aminothiophenol (4-ATP) modified surface enhanced Raman spectroscopy probe GNS @ Ag-ATP, and comprising the following steps:
adding 20 mu L of Raman reporter 4-aminothiophenol (4-ATP, 10 mM) into 5 mL of GNS @ Ag prepared in the second step, and carrying out ultrasonic treatment at 30-40 ℃ for 0.5-1 hour; centrifuging at 8000-9000 rpm for 5-10 minutes, and removing the supernatant to obtain the surface enhanced Raman spectroscopy substrate material modified with the reporter molecule;
step four, resuspending the reporter-modified surface enhanced Raman spectrum substrate material obtained in the step three with cysteamine (1-5 mM), performing ultrasonic reaction at 30-40 ℃ for 1-2 hours, centrifuging at 9000 rpm for 8-10 minutes, removing supernatant, and performing ddH (distilled water) twice2O Wash 2 times, 1 mL ddH2And O, re-suspending to obtain the surface enhanced Raman spectroscopy probe GNS @ Ag-ATP, and storing the probe GNS @ Ag-ATP at 4 ℃ for later use.
The observation result of the GNS @ Ag-ATP by a transmission electron microscope is shown in FIG. 1, the detection result of the ultraviolet-visible light absorption spectrum is shown in FIG. 2, and the detection result of the Raman spectrum under the conditions of the laser wavelength of 785 nm, the power of 4.5 mW and the integration time of 10 seconds is shown in FIG. 3.
Step five, preparing a surface enhanced Raman spectroscopy probe GNS @ Ag-ATP-FA labeled by targeting recognition of the molecularly imprinted FA, and specifically comprising the following steps:
1 mL of GNS @ Ag-ATP prepared in the fourth step was centrifuged at 9000 rpm for 8 minutes, the supernatant was removed, and the mixture was resuspended in 2-morpholinoethanesulfonic acid (MES) solution (0.1M, pH5.0 to 6.0) and mixed with 4 mL of folic acid (30. mu.g/mL) containing EDC (2 mM) and MES (0.1M, pH5.0 to 6.0), and reacted at room temperature for 1 hour(ii) a Centrifuging at 9000 rpm for 8 min, removing supernatant, and redistilling water ddH2O Wash 2 times, 1 mL ddH2And O, re-suspending to obtain the folate-labeled surface enhanced Raman spectroscopy probe GNS @ Ag-ATP-FA.
The detection result of the ultraviolet-visible light absorption spectrum of the folate-labeled surface-enhanced Raman spectroscopy probe GNS @ Ag-ATP-FA is shown in FIG. 2, and the detection result of the Raman spectrum under the conditions of the laser wavelength of 785 nm, the power of 4.5 mW and the integration time of 10 seconds is shown in FIG. 3.
In this embodiment, the folic acid FA labeled surface-enhanced raman spectroscopy probe GNS @ Ag-ATP-FA takes gold-silver nano composite particles with a gold nano star as a core and silver as a shell as a surface-enhanced raman spectroscopy substrate material for signal amplification, a reporter molecule is modified on the surface of the surface-enhanced raman spectroscopy substrate material for signal detection, a cysteamine molecule is modified to stabilize the nano particles and can be connected with a targeted recognition folate FA molecule, and the targeted recognition molecule is connected to capture a target molecule.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (6)

1. A preparation method of a surface enhanced Raman spectrum probe is characterized by comprising the following steps:
step one, synthesizing a gold nano star by a gold seed growth method;
coating a silver shell on the surface of the gold nano star obtained in the step one by an ascorbic acid reduction silver nitrate method to synthesize a surface enhanced Raman spectroscopy substrate material with a gold-silver nano composite structure;
performing ultrasonic reaction on the surface-enhanced Raman spectrum substrate material obtained in the step two and a Raman reporter molecule at the temperature of 30-40 ℃ for 0.5-1 hour to obtain a surface-enhanced Raman spectrum substrate material modified with the reporter molecule;
step four, carrying out ultrasonic reaction on the surface enhanced Raman spectrum substrate material modified with the reporter molecule obtained in the step three and cysteamine molecules at the temperature of 30-40 ℃ for 1-2 hours to obtain nanoparticles which are stable surface enhanced Raman spectrum probes;
and step five, connecting the targeted recognition molecules to the surface of the stable surface-enhanced Raman spectrum probe prepared in the step four through an EDC (EDC) activation reaction of 1-ethyl- (3-dimethylaminopropyl) carbonyldiimine to obtain the specific surface-enhanced Raman spectrum probe.
2. The method for preparing the surface-enhanced Raman spectroscopy probe according to claim 1, wherein the surface-enhanced Raman spectroscopy substrate material is gold-silver nanocomposite particles having a gold nanostar as a core and silver as a shell.
3. The method for preparing a surface-enhanced Raman spectroscopy probe of claim 1, wherein in step three, the Raman reporter is a thiol-bearing Raman reporter comprising 4-aminothiophenol 4-ATP and 4-aminobenzoic acid.
4. The method for preparing the surface-enhanced Raman spectroscopy probe of claim 1, wherein in the fifth step, the target recognition molecule is a molecule which carries or is modified with carboxyl, and comprises folic acid FA, an antibody and a nucleic acid aptamer.
5. The surface-enhanced Raman spectroscopy probe according to any one of claims 1 to 4, wherein the surface-enhanced Raman spectroscopy probe is prepared by the steps of:
step one, synthesizing a gold nano star by a gold seed growth method, comprising the following steps:
(1) preparing gold seeds: 6 mL of a 1% trisodium citrate solution are quickly added to 40 mL of boiling 1mM HAuCl tetrachloroaurate4Continuously heating and stirring the solution for 15 minutes until the color of the solution becomes wine red, which indicates that the gold seeds are successfully generated;
(2) and (3) synthesizing GNS: mixing 300 μ L of gold seed prepared in (1) with 30 mL of 0.1 mM HAuCl tetrachloroaurate containing 300. mu.L of 1M HCl4Mixing, adding 150 μ L6 mM silver nitrate AgNO3Mixing with 150 μ L and 0.1M ascorbic acid AA, rapidly mixing, and rapidly changing the solution color into dark green to obtain gold nano star GNS; subsequently, 300 μ L, 0.1M cetyltrimethylammonium bromide CTAB was added and mixed well to stabilize GNS; centrifuging at 8000-9000 rpm for 10-15 minutes at 4 ℃, removing supernatant, adding 30 mL of 1mM CTAB for resuspension to obtain a gold nano star GNS solution, and storing at 4 ℃ for later use;
step two, preparing a surface enhanced Raman spectroscopy substrate material with a gold-silver nano composite structure:
adding 20 μ L, 0.1M ascorbic acid AA and 20 μ L, 0.1M silver nitrate AgNO into 5 mL of gold nano star GNS solution synthesized in the first step3After uniformly mixing, adding 5-10 mu L of ammonia water, rapidly and uniformly mixing for 5-10 minutes, and synthesizing to obtain a surface-enhanced Raman spectroscopy substrate material GNS @ Ag of a gold-silver nano composite structure with a gold nano star as a core and silver as a shell, wherein the solution is brown;
step three, preparing a surface enhanced Raman spectroscopy substrate material modified with a reporter molecule 4-aminothiophenol 4-ATP:
adding 20 mu L of 10 mM Raman reporter molecule 4-aminothiophenol 4-ATP into 5 mL of GNS @ Ag prepared in the second step, and carrying out ultrasonic treatment at 30-40 ℃ for 0.5-1 hour; centrifuging at 8000-9000 rpm for 5-10 minutes, and removing the supernatant to obtain the surface enhanced Raman spectroscopy substrate material modified with the reporter molecule;
step four, resuspending the reporter-modified surface enhanced Raman spectrum substrate material obtained in the step three with 1-5 mM cysteamine, performing ultrasonic reaction at 30-40 ℃ for 1-2 hours, centrifuging at 9000 rpm for 8-10 minutes, removing supernatant, and performing ddH (distilled water) twice2O Wash 2 times, 1 mL ddH2O resuspending, namely obtaining a surface enhanced Raman spectroscopy probe GNS @ Ag-ATP, and storing the surface enhanced Raman spectroscopy probe GNS @ Ag-ATP at 4 ℃ for later use;
step five, preparing a surface enhanced Raman spectroscopy probe GNS @ Ag-ATP-FA labeled by targeting recognition of the molecularly imprinted FA, and specifically comprising the following steps:
1 mL of GNS @ Ag-ATP prepared in step four above was centrifuged at 9000 rpm for 8 minutes, the supernatant was removed, and the mixture was washed with 0.1M,Resuspending 2-morpholine ethanesulfonic acid MES solution with the pH value of 5.0-6.0, uniformly mixing with 4 mL of 2 mM MES containing EDC and 0.1M and with the pH value of 5.0-6.0 and the concentration of 30 mu g/mL folic acid, and reacting for 1 hour at room temperature; centrifugation at 9000 rpm for 8 min, removal of supernatant, ddH2O Wash 2 times, 1 mL ddH2And O, re-suspending to obtain the folate-labeled surface enhanced Raman spectroscopy probe GNS @ Ag-ATP-FA.
6. A surface-enhanced Raman spectroscopy probe prepared according to the method of any one of claims 1 to 5.
CN202111308137.XA 2021-11-05 2021-11-05 Preparation method of surface-enhanced Raman spectrum probe and product thereof Pending CN114045164A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111308137.XA CN114045164A (en) 2021-11-05 2021-11-05 Preparation method of surface-enhanced Raman spectrum probe and product thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111308137.XA CN114045164A (en) 2021-11-05 2021-11-05 Preparation method of surface-enhanced Raman spectrum probe and product thereof

Publications (1)

Publication Number Publication Date
CN114045164A true CN114045164A (en) 2022-02-15

Family

ID=80207712

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202111308137.XA Pending CN114045164A (en) 2021-11-05 2021-11-05 Preparation method of surface-enhanced Raman spectrum probe and product thereof

Country Status (1)

Country Link
CN (1) CN114045164A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115184339A (en) * 2022-09-08 2022-10-14 海澳华(黑龙江)生物医药技术有限公司 Method for rapidly detecting viruses based on portable Raman spectrometer
CN115531539A (en) * 2022-09-19 2022-12-30 海南大学 Near-infrared SERS signal enhanced nano-probe, preparation method thereof and application thereof in integrated diagnosis and treatment of infectious bacteria
CN115814112A (en) * 2022-12-05 2023-03-21 南京师范大学 Preparation method and application of Raman minimally invasive probe for detecting glutathione

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109900911A (en) * 2019-03-11 2019-06-18 西安交通大学 A method of hepatic carcinoma marker AFP is detected with nuclear shell structure nano star
CN110618123A (en) * 2019-09-11 2019-12-27 亳州市新健康科技有限公司 Efficient surface-enhanced Raman scattering substrate material and preparation method thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109900911A (en) * 2019-03-11 2019-06-18 西安交通大学 A method of hepatic carcinoma marker AFP is detected with nuclear shell structure nano star
CN110618123A (en) * 2019-09-11 2019-12-27 亳州市新健康科技有限公司 Efficient surface-enhanced Raman scattering substrate material and preparation method thereof

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115184339A (en) * 2022-09-08 2022-10-14 海澳华(黑龙江)生物医药技术有限公司 Method for rapidly detecting viruses based on portable Raman spectrometer
CN115184339B (en) * 2022-09-08 2022-12-23 海澳华(黑龙江)生物医药技术有限公司 Method for rapidly detecting viruses based on portable Raman spectrometer
CN115531539A (en) * 2022-09-19 2022-12-30 海南大学 Near-infrared SERS signal enhanced nano-probe, preparation method thereof and application thereof in integrated diagnosis and treatment of infectious bacteria
CN115531539B (en) * 2022-09-19 2023-09-15 海南大学 Near-infrared SERS signal enhanced nano probe, preparation method thereof and application thereof in integrated diagnosis and treatment of infectious bacteria
CN115814112A (en) * 2022-12-05 2023-03-21 南京师范大学 Preparation method and application of Raman minimally invasive probe for detecting glutathione
CN115814112B (en) * 2022-12-05 2024-02-02 南京师范大学 Preparation method and application of Raman minimally invasive probe for detecting glutathione

Similar Documents

Publication Publication Date Title
Pu et al. Elucidating the growth mechanism of plasmonic gold nanostars with tunable optical and photothermal properties
CN114045164A (en) Preparation method of surface-enhanced Raman spectrum probe and product thereof
Khlebtsov et al. Gap-enhanced Raman tags: fabrication, optical properties, and theranostic applications
Li et al. Surface-enhanced Raman scattering active plasmonic nanoparticles with ultrasmall interior nanogap for multiplex quantitative detection and cancer cell imaging
Yin et al. Prussian blue as a highly sensitive and background-free resonant Raman reporter
Zhang et al. Biosynthesis of gold nanoparticles using chloroplasts
Israelsen et al. Nanoparticle Properties and Synthesis Effects on Surface‐Enhanced Raman Scattering Enhancement Factor: An Introduction
Khlebtsov Determination of size and concentration of gold nanoparticles from extinction spectra
Vo-Dinh et al. Plasmonic nanoprobes: from chemical sensing to medical diagnostics and therapy
Sharifi et al. Plasmonic and chiroplasmonic nanobiosensors based on gold nanoparticles
Alvarez-Puebla et al. SERS-fluorescent encoded particles as dual-mode optical probes
Jayan et al. Mesoporous silica coated core-shell nanoparticles substrate for size-selective SERS detection of chloramphenicol
KR101230180B1 (en) Surface enhanced spectroscopy-active composite nanoparticles
Liu et al. Recent Progress on Gold‐Nanocluster‐Based Fluorescent Probe for Environmental Analysis and Biological Sensing
Krajczewski et al. Shell-isolated nanoparticle-enhanced Raman spectroscopy
Yaraki et al. Recent advances in metallic nanobiosensors development: colorimetric, dynamic light scattering and fluorescence detection
Bhardwaj et al. Gold nanobipyramids integrated ultrasensitive optical and electrochemical biosensor for Aflatoxin B1 detection
Liu et al. Ultrasensitive and facile detection of multiple trace antibiotics with magnetic nanoparticles and core-shell nanostar SERS nanotags
Liu et al. A novel fluorescent probe for ascorbic acid based on seed-mediated growth of silver nanoparticles quenching of carbon dots fluorescence
Tai et al. Gold nanoparticles based optical biosensors for cancer biomarker proteins: a review of the current practices
KR102257511B1 (en) Magnetic-Optical Composite Nanoparticles
Chen et al. Emergence of surface-enhanced Raman scattering probes in near-infrared windows for biosensing and bioimaging
CN108535236A (en) A method of based on dual amplification SERS signal system super sensitivity detection miRNA
Liu et al. Colorimetry/SERS dual-sensor of H2O2 constructed via TMB–Fe3O4@ AuNPs
Wen et al. Surface‐Enhanced Raman Probes Based on Gold Nanomaterials for in vivo Diagnosis and Imaging

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination