CN114041623B - Tobacco characteristic aroma component, preparation method and application thereof - Google Patents
Tobacco characteristic aroma component, preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
- A24B15/26—Use of organic solvents for extraction
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/10—Chemical features of tobacco products or tobacco substitutes
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/28—Treatment of tobacco products or tobacco substitutes by chemical substances
- A24B15/30—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/14—Forming reconstituted tobacco products, e.g. wrapper materials, sheets, imitation leaves, rods, cakes; Forms of such products
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Abstract
The invention provides a tobacco characteristic aroma component which comprises farnesol, geraniol, plant alcohol, palmitic acid and solanone; the aroma component has rice wine aroma, fresh sweet aroma and flower aroma. The invention also discloses a preparation method and application of the characteristic aroma component of the tobacco. The aroma component of the invention is applied to tobacco, and can improve the quality of tobacco products.
Description
Technical Field
The invention relates to the field of tobacco, in particular to a characteristic aroma component of tobacco and a preparation method and application thereof
Background
The tobacco aroma highlights the characteristics of tobacco leaves, embodies the aroma and the style characteristics of flue-cured tobacco, and the tobacco aroma substance is a natural spice of the tobacco origin, is an ideal flavoring agent, can be added into cigarettes, cigarette paper, filter tip materials and plasticizers to increase the aroma of the cigarettes, improve the sensory quality of the cigarettes and the original aroma of the tobacco, increase the satisfaction, simultaneously improve the comprehensive utilization rate of tobacco raw materials, reduce the cost and have wider market and application prospects. The dry tobacco is similar to Sichuan tobacco, the tobacco leaf is thicker, the leaf shape is wide and greasy, the leaf edge is wavy, the leaf stem is thick, the leaf surface is ruddy and smooth, the oil content is sufficient, the toughness is good, the flavor is mellow, the sugar content is low, the combustibility is good, the ignition is fast, the tobacco leaves are grayish white, and the quality is very good. Yunnan Huizzilian tobacco (also called Huizzilian leaf tobacco, sun-cured tobacco and native tobacco) has a long planting and processing history, is economic crops in Huizzicounty, and is brought from Sichuan Jintang, shi 37025with Han nationalities in Qingzhuangyang years. The soil belongs to a valley trough area of the le industry, the natural condition is suitable, the soil is subacid purple soil, the texture and the structure are good, and the nutrients are coordinated. Few studies on the aroma components in the Huizhou tobacco are reported at present.
Disclosure of Invention
The invention provides a tobacco characteristic aroma component, a preparation method and application thereof for the first time.
The invention is realized by the following technical scheme.
The invention discloses a tobacco characteristic aroma component in a first aspect, wherein the aroma component comprises farnesol, geraniol, phytol, palmitic acid and solanone; the aroma component has rice wine aroma, fresh and sweet aroma and flower aroma; the fragrance is rich, the experience feeling is good, and the irritation is small; the farnesol, geraniol and plant alcohol are main aroma components, and the palmitic acid and the solanone are modified aroma components.
Preferably, in the aroma component, farnesol is 0.56-0.60 wt%, geraniol is 0.12-0.19 wt%, plant alcohol is 11.98-12.07 wt%, palmitic acid is 1.60-1.65 wt%, and solanone is 0.72-0.75 wt%.
The second aspect of the invention discloses a preparation method of the aroma component, which comprises the following steps:
(1) Extracting tobacco raw material with solvent, cooling the extract, filtering, and concentrating under reduced pressure to obtain extract;
(2) Separating and purifying the extract obtained in the step (1) by macroporous adsorption resin column chromatography to obtain a target section I;
(3) And (3) separating and purifying the target section I obtained in the step (2) by using a gel chromatographic column to obtain the tobacco characteristic aroma component.
Preferably, the solvent in the step (1) is ethanol solution, and the volume fraction of the ethanol solution is 70-95%; the mass volume ratio of the raw material of the dry tobacco to the ethanol solution is 1 to (8-20), and the unit is g/ml; the extraction method is water bath hot reflux extraction, the water bath temperature is 50-80 ℃, the extraction is carried out for 2-3 times, and the extraction time is 1-2 h each time; the raw material of the dry tobacco is preferably Yunnan Huizue dry tobacco; the concentration under reduced pressure is carried out until the organic solvent smell disappears and the relative density is below 1.20 (measured at room temperature).
Preferably, the macroporous adsorbent resin in the step (2) is selected from any one of AB-8, D101, DA-201, HPD-700, HP-20, HPD-722, HPD-300, HPD-200A, HPD-200B, HPD-100, HPD400, ADS-21 or HPD-850, preferably HPD-100.
Preferably, the macroporous adsorbent resin column chromatography separation and purification step in the step (2) is as follows: dissolving and filtering with water, adding into a macroporous adsorption resin column, eluting with water for 8-12 BV at a flow rate of 0.2-1.5 BV/h (BV refers to the volume of the column), removing impurities, eluting with 90-95 vol% ethanol for 15-20 BV, collecting the part of eluent, and concentrating under reduced pressure to obtain a target section I; preferably, the ratio of height to diameter of the macroporous adsorbent resin column is (5-10) 1, such as 6; the amount of the macroporous resin is preferably 10 to 20 times, for example, 11 times, 12 times, 13 times, 14 times, 15 times, 16 times, 17 times, 18 times or 19 times, of the mass of the extract, but the macroporous resin is not limited to the above-mentioned values, and other values not listed in the above-mentioned range are also applicable. The macroporous adsorption resin used in the application can treat a large amount of tobacco aroma components containing the aroma components after alcohol extraction, quickly remove substances such as polysaccharide and water-soluble pigment, is beneficial to further separation and purification of subsequent aroma components, can realize industrial-grade treatment by utilizing the macroporous adsorption resin, and has short treatment time period.
Preferably, the gel chromatographic column packing in the step (3) is selected from any one of Sephadex LH-20, sephadex G-25 and Sephadex G-10, and is preferably Sephadex LH-20.
Preferably, the gel chromatographic column separation and purification step in step (3) is: dissolving and filtering a target section I by using ethanol, adding the obtained solution into a gel chromatographic column, eluting the gel chromatographic column by using absolute ethanol at the flow rate of 2-5 drops/second, collecting fractions for 5-7h, and concentrating the fractions under reduced pressure to obtain the characteristic aroma components of the tobacco, wherein the filtering in the steps mainly removes insoluble impurities, and can be filtered by using a 0.6-0.8 mu m microporous filter membrane; the temperature of the reduced pressure concentration in the above step is 50-65 ℃, such as 50 ℃, 55 ℃, 60 ℃ or 65 ℃, but not limited to the enumerated values, and other unrecited values in the numerical range are also applicable; the vacuum degree of the reduced pressure concentration is-0.08 MPa to-0.1 MPa, such as-0.08 MPa, -0.085MPa, -0.09MPa or-0.095 MPa, and the like, but the vacuum degree is not limited to the enumerated values, and other non-enumerated values in the numerical value range are also applicable; the gel filler is preferably used in an amount of 30 to 50 times, such as 32 times, 34 times, 36 times, 38 times, 40 times, 42 times, 44 times, 46 times, or 48 times, the mass of the target section I, but is not limited to the recited values, and other values not recited in the range of values are also applicable; the gel column aspect ratio is 50 to 120, such as 60.
The invention discloses the application of the tobacco characteristic aroma component to tobacco flavoring and upgrading in a third aspect.
The invention has the beneficial effects that:
1. the characteristic aroma components of the dry tobacco have obvious rice wine aroma, fresh and sweet aroma and flower aroma under certain different proportions, and the dry tobacco is rich in aroma, less in miscellaneous gas, good in experience feeling and small in irritation. The aromatic substance is added into the application of perfuming and quality improvement of cigarettes, electronic cigarettes and the like, so that the aromatic amount can be increased, the aroma can be improved, and the quality of tobacco products such as cigarettes can be improved.
2. The tobacco characteristic aroma components of the dry tobacco comprise farnesol, geraniol, phytol and the like, and in addition, palmitic acid and solanone are contained to play a role in modifying aroma, and the tobacco characteristic aroma components are added into tobacco as additives to increase the aroma amount, improve the aroma note and improve the quality of tobacco products such as cigarettes. The aroma components have obvious rice wine aroma in a certain content range, and the rice wine aroma has the advantages of no offensive odor, good experience feeling, small irritation and wide application prospect.
3. The preparation method of the invention adopts ethanol water bath hot reflux extraction, and the macroporous adsorption resin is separated and combined with the gel chromatographic column, so that the tobacco extract can be quickly prepared. The preparation method has simple process and easy realization, and the used macroporous resin and gel can be repeatedly applied for many times, thereby being economic and efficient.
Detailed Description
The technical solution of the present invention is further described below by way of specific embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention. The procedures, conditions, reagents, experimental methods and the like for carrying out the present invention are general knowledge and common general knowledge in the art except for the contents specifically mentioned below, and the present invention is not particularly limited. The experimental procedures in each example, in which the specific conditions are not specified, are generally carried out under the conventional conditions or under the conditions recommended by the manufacturers. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In case of conflict, the present specification, including definitions, will control. The tobacco leaves in the following examples are the upper leaves of Yunnan Qujing Oze tobacco leaves.
Example 1
(1) 50g of tobacco leaves are soaked in 95% ethanol for 2h, the material-liquid ratio is 1.
(2) Dissolving the above obtained extract with water, filtering, loading on macroporous adsorbent resin column HPD-100 with an amount of 20 times of the extract mass, eluting with water for 10 column volumes at a flow rate of 1.5BV/h, eluting with 95% ethanol for 20 column volumes, collecting the eluate, concentrating under reduced pressure to obtain target section I of 5.9g
(3) Dissolving the target section I with ethanol, filtering, loading onto gel chromatographic column (SephadexLH-20 as filler, 40 times of the target section I in mass), collecting eluate for 5-7 hr at flow rate of 2 drops/s, concentrating under reduced pressure, and drying to obtain 0.27g of fragrant component with dry tobacco characteristics.
The obtained tobacco characteristic aroma components have obvious rice wine aroma, fresh and sweet aroma and flower aroma, no miscellaneous gas, good experience feeling and small irritation, the aroma components, the percentage content and the relative aroma activity value (ROAV) are measured and analyzed, the result is shown in table 1, and the measuring method is as follows:
taking 15.6mg of tobacco characteristic aroma component obtained by the preparation, adding acetone to dissolve and fix the volume to 1.5ml, filtering by an organic needle filter, transferring into a chromatographic sample injection vial, and carrying out GC-MS analysis.
Gas chromatography-mass spectrometry conditions: the instrument was an Agilent 7890A-5975C GC-MS and the capillary column was an HP-5MS (30 m. Times.0.25 mm. Times.0.25 μm) from Agilent J & W Scientific.
The instrument parameters were set as: the sample port temperature is 250 ℃, the EI ion source temperature is 230 ℃, the quadrupole temperature is 150 ℃, high-purity nitrogen (purity is more than 99.999%) is used as carrier gas, the column flow rate is 1mL/min, split-flow sample injection is carried out, the split-flow ratio is 10, the sample injection amount is 1 mu L, and the solvent delay is 3min.
The temperature rising procedure is as follows: the initial temperature was 50 deg.C, maintained at 1min, the rate of 5 deg.C/min was increased to 200 deg.C, then increased to 260 deg.C at 10 deg.C/min, and maintained for 5min. And performing mass spectrum detection in a full-scanning mode, wherein the mass spectrum detection range is 35-450 (m/z).
And continuous sample analysis is performed in a random sequence, so that influence caused by signal fluctuation of an instrument is avoided.
Inquiry of threshold value and calculation of relative fragrance activity value.
Sensory threshold queries use thresholds provided by the Leffingwell & Associates website and thresholds in the literature, with preference to threshold data for the latest year. The references are as follows:
(1)Van Gemert L J.Odour thresholds:compilations of odour threshold values in air，water and other media[M].Houten:Oliemans Punter&Partners BV，2011.
(2)Davis D L，NielsenM T.Tobacco:production，chemistry and technology[M].Oxford:Blackwell Science Ltd.，1999.
(3) Liu Shuo, research on main aroma components of tobacco leaves, cigarettes and tobacco additives based on aroma vitality values [ D ]. Yunnan, university of Kunming science and technology, 2020.
By relative odourThe ROVA method, which defines the component ROAV that contributes most to the flavour of a sample max =100, other aroma components are according to the formula:
C i 、T i is the relative percentage content of each aroma component and the corresponding sensory threshold; c max 、T max The relative percentage of the component that contributed the most to the overall aroma of the sample and the corresponding sensory threshold, respectively.
TABLE 1 percentage of aroma components and ROAV value in example 1
| Retention time | Name of Compound | Percent (%) | Threshold value (mg/kg) | ROAV value |
| 13.73 | Farnesol | 0.56 | 0.02 | 53.70 |
| 24.09 | Geraniol | 0.12 | 0.075 | 4.30 |
| 25.37 | Plant alcohol | 11.98 | 12 | 1.70 |
| 25.58 | Palmitic acid | 1.60 | 10 | 0.26 |
| 12.26 | Solanone | 0.72 | 1.82 | 0.734 |
From table 1 above, it can be seen that the most significant ROVA value is farnesol with faint scent, which plays a role of main fragrance, and solanone and palmitic acid play a role of modifying fragrance in the fragrant components, and the combination of the components makes the fragrant components have obvious fresh and sweet fragrance, licorice fragrance and flower fragrance, and has no offensive odor, good experience and little irritation.
Example 2
(1) 50g of tobacco leaves are soaked in 95% ethanol for 2h, the material-liquid ratio is 1. Then, the extracted solution is distilled and concentrated under reduced pressure at 50 ℃ to obtain tan viscous liquid, and 9.5 g of extract is obtained after cooling.
(2) Dissolving the above extract with water, filtering, loading onto macroporous adsorbent resin column (HPD-100, the amount is 10 times of the extract mass), eluting with water for 8 column volumes at a flow rate of 1.5BV/h, eluting with 90% ethanol for 20 column volumes, collecting the eluate, and concentrating under reduced pressure to obtain segment I of 6.6g
(3) Dissolving the target section I with ethanol, filtering, adding gel chromatographic column filler (SephadexLH-20, the amount is 30 times of the target section I in mass), collecting part of eluent for 5-7h, concentrating under reduced pressure, and drying to obtain 0.32g of fragrant component with characteristics of sweet and dry tobacco.
The obtained tobacco characteristic aroma components have obvious fresh and sweet aroma, liquorice aroma and flowery flavor, no miscellaneous gas, good experience feeling, small irritation, aroma components and content, and relative aroma activity value (ROAV) shown in table 2, and the determination and analysis method is the same as that of example 1
TABLE 2 percentage of aroma components and ROAV value in example 2
| Retention time | Name of the Compound | Percentage content (%) | Threshold value (mg/kg) | ROAV value |
| 13.73 | Farnesol | 0.57 | 0.02 | 53.70 |
| 24.09 | Geraniol | 0.15 | 0.075 | 4.35 |
| 25.37 | Plant alcohol | 12.00 | 12 | 1.75 |
| 25.58 | Palmitic acid | 1.62 | 10 | 0.29 |
| 12.26 | Solanone | 0.73 | 1.82 | 0.734 |
Example 3
(1) 50g of tobacco leaves are soaked in 95% ethanol for 1h, the material-liquid ratio is 1.
(2) Dissolving the above obtained extract with water, filtering, loading onto macroporous adsorbent resin column (HPD-100, the amount is 15 times of the extract mass), eluting with water for 8 column volumes at flow rate of 1.5BV/h, eluting with 95% ethanol for 15 column volumes, collecting the eluate, concentrating under reduced pressure to obtain target section I of 7.3g
(3) Dissolving target section I with ethanol, filtering, loading onto gel chromatographic column (SephadexLH-20, the amount is 50 times of target section I), collecting eluate for 5-7 hr at flow rate of 2 drops/s, concentrating under reduced pressure, and drying to obtain tobacco component with fragrance of 0.37g
The obtained characteristic aroma components of the drought tobacco have obvious fresh and sweet aroma, liquorice aroma and flowery flavor, no foreign flavor, good experience feeling and small irritation, the aroma components, the percentage content and the relative aroma activity value (ROAV) are shown in a table 3, and the determination and analysis method is the same as that of the embodiment 1
TABLE 3 aroma content and ROAV value in example 3
| Retention time | Name of Compound | Percentage content (%) | Threshold value (mg/kg) | ROAV value |
| 13.73 | Farnesol | 0.58 | 0.02 | 53.70 |
| 24.09 | Geraniol | 0.17 | 0.075 | 4.40 |
| 25.37 | Plant alcohol | 12.05 | 12 | 1.789 |
| 25.58 | Palmitic acid | 1.64 | 10 | 0.294 |
| 12.26 | Solanone | 0.74 | 1.82 | 0.736 |
Example 4
(1) 50g of tobacco leaves are soaked in absolute ethyl alcohol for 2 hours, the material-liquid ratio is 1.
(2) Dissolving the above obtained extract with water, filtering, loading onto macroporous adsorbent resin column (HPD-100, the amount is 15 times of the extract mass), eluting with water for 10 column volumes at flow rate of 1.5BV/h, eluting with 95% ethanol for 15 column volumes, collecting the eluate, concentrating under reduced pressure to obtain section I of 8.0g
(3) And (3) dissolving and filtering the sample II, loading the sample II on a gel chromatographic column (SephadexLH-20, the dosage of which is 40 times of the mass of the target section I), collecting 5-7h of eluent at the flow rate of 2 drops/second, and performing reduced pressure concentration and drying to obtain 0.42g of the dry tobacco characteristic aroma component with fresh and sweet aroma.
The obtained tobacco characteristic aroma components have obvious rice wine aroma, no offensive odor, good experience feeling, small irritation, aroma components, content and relative aroma activity value (ROAV) shown in Table 4, and the determination and analysis method is the same as that of example 1, table 4, and example 4
| Retention time | Name of Compound | Percentage content (%) | Threshold value (mg/kg) | ROAV value |
| 13.73 | Farnesol | 0.60 | 0.02 | 53.804 |
| 24.09 | Geraniol | 0.19 | 0.075 | 4.543 |
| 25.37 | Plant alcohol | 12.07 | 12 | 1.804 |
| 25.58 | Palmitic acid | 1.65 | 10 | 0.296 |
| 12.26 | Solanone | 0.75 | 1.82 | 0.739 |
Comparative example 1 (step 2 macroporous resin adsorption step less than example 1)
(1) 50g of tobacco leaves are soaked in 95% ethanol for 2h, the material-liquid ratio is 1, reflux extraction is carried out in a water bath, the temperature of the water bath is 60 ℃, and extraction is repeated twice. Then, the extracted solution is distilled and concentrated under reduced pressure at 50 ℃ to obtain tan viscous liquid, and 9.1 g of extract is obtained after cooling.
(2) Dissolving the extract with ethanol, filtering, separating with gel chromatographic column (SephadexLH-20 as filler, 360 g), collecting 5-7 hr fraction with mobile phase of anhydrous ethanol and flow rate of 2 drops/s, concentrating under reduced pressure, and drying to obtain tobacco aroma component 0.6.
The obtained tobacco characteristic aroma components also have certain rice wine aroma and fresh and sweet aroma, but simultaneously have more miscellaneous gas and strong irritation, the aroma components, the relative contents and the relative aroma activity value (ROAV) are shown in a table 5, and the determination and analysis method is the same as that of the embodiment 1
TABLE 5 comparative example 1 aroma components, contents and ROAV values
In comparative example 1, compared with example 1, although the content of the aroma-causing substances such as farnesol, geraniol, and phytol was increased, and a rice wine aroma appeared, the aroma-causing substances contained impurity substances such as kame and neophytadiene, which contained strong smell of wax fat, heavy offensive odor, and strong irritation. The macroporous resin adsorption can remove not only impurities such as polysaccharide, amino acid, pigment and the like, but also impurity substances such as kame, neophytadiene and the like, is convenient for subsequent gel chromatographic separation, can better enrich fragrant substances with sweet and brown fragrance, and reduces the content of other miscellaneous gases.
Application example 1
The aroma components prepared in the above examples and comparative examples are dissolved by water and propylene glycol and sprayed on cured tobacco leaves to prepare cigarette evaluation. The results were: the tobacco extracts of the embodiment 1, the embodiment 2, the embodiment 3 and the embodiment 4 are added, so that the aroma richness of the sample is improved, the rice wine aroma is generated, the miscellaneous gas is not generated, the satisfaction is higher, and the integral smoking quality of the flue-cured tobacco is effectively improved.
The above description is only for the purpose of illustrating the present invention and is not intended to limit the present invention in any way, and the present invention is not limited to the above description, but rather should be construed as being limited only by the claims, and all changes and modifications that fall within the spirit and scope of the invention are therefore intended to be embraced by the appended claims.
Claims (5)
1. A tobacco smoke-characteristic aroma constituent, wherein the aroma constituent comprises farnesol, geraniol, plant alcohol, palmitic acid and solanone; the aroma component has rice wine aroma, fresh and sweet aroma and flower aroma; in the aroma component, 0.56-0.60 wt% of farnesol, 0.12-0.19 wt% of geraniol, 11.98-12.07 wt% of plant alcohol, 1.60-1.65 wt% of palmitic acid and 0.72-0.75 wt% of solanone are contained;
the preparation method of the aroma component comprises the following steps:
(1) Extracting tobacco raw material with solvent, cooling the extract, filtering, and concentrating under reduced pressure to obtain extract;
(2) Separating and purifying the extract obtained in the step (1) by macroporous adsorption resin column chromatography to obtain a target section I; the macroporous adsorption resin column chromatography separation and purification steps are as follows: dissolving and filtering with water, adding into a macroporous adsorption resin column, eluting with water at a flow rate of 0.2-1.5 BV/h for 8-12 BV to remove impurities, eluting with 90-95 vol% ethanol for 15-20 BV, collecting the part of eluent, and concentrating under reduced pressure to obtain a target section I;
(3) Separating and purifying the target section I obtained in the step 2 by using a gel chromatographic column to obtain the characteristic aroma components of the tobacco; the gel chromatographic column separation and purification steps are as follows: dissolving the target section I with ethanol, filtering, adding the solution into a gel chromatographic column, eluting with absolute ethanol at the flow rate of 2-5 drops/second, collecting the flow parts for 5-7 hours, and concentrating under reduced pressure to obtain the characteristic aroma components of the tobacco.
2. The tobacco characteristic perfuming ingredient according to claim 1, wherein the solvent in step (1) is ethanol solution, and the volume fraction of the ethanol solution is 70-95%; the mass volume ratio of the raw material of the dry tobacco to the ethanol solution is 1: 8-20, the extraction method is water bath hot reflux extraction, the water bath temperature is 50-80 ℃, the extraction is carried out for 2-3 times, and the extraction time is 1-2 h each time.
3. A dry tobacco specific aroma component according to claim 1, wherein the macroporous adsorbent resin of step (2) is selected from any one of AB-8, D101, DA-201, HPD-700, HP-20, HPD-722, HPD-300, HPD-200A, HPD-200B, HPD-100, HPD400, ADS-21 or HPD-850.
4. The tobacco smoke characteristic perfuming composition according to claim 1, wherein the gel chromatographic column packing of the step (3) is selected from any one of SephadexLH-20, sephadexG-25, sephadex G-10.
5. Use of a tobacco flavoring and upgrading ingredient according to the dry tobacco characteristics of claim 1.
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