CN114019054A - Method for calculating concentration of traditional Chinese medicine components in interface layer of nanofiltration membrane - Google Patents

Method for calculating concentration of traditional Chinese medicine components in interface layer of nanofiltration membrane Download PDF

Info

Publication number
CN114019054A
CN114019054A CN202111316854.7A CN202111316854A CN114019054A CN 114019054 A CN114019054 A CN 114019054A CN 202111316854 A CN202111316854 A CN 202111316854A CN 114019054 A CN114019054 A CN 114019054A
Authority
CN
China
Prior art keywords
interface layer
concentration
membrane
chinese medicine
traditional chinese
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN202111316854.7A
Other languages
Chinese (zh)
Other versions
CN114019054B (en
Inventor
李存玉
支兴蕾
彭国平
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanjing University of Chinese Medicine
Original Assignee
Nanjing University of Chinese Medicine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanjing University of Chinese Medicine filed Critical Nanjing University of Chinese Medicine
Priority to CN202111316854.7A priority Critical patent/CN114019054B/en
Publication of CN114019054A publication Critical patent/CN114019054A/en
Application granted granted Critical
Publication of CN114019054B publication Critical patent/CN114019054B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/74—Optical detectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/06—Preparation
    • G01N2030/065—Preparation using different phases to separate parts of sample
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A20/00—Water conservation; Efficient water supply; Efficient water use
    • Y02A20/124—Water desalination
    • Y02A20/131—Reverse-osmosis

Landscapes

  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)

Abstract

The invention relates to a method for calculating the concentration of traditional Chinese medicine components in an interface layer of a nanofiltration membrane, which establishes a calculation method based on the interface mass transfer basic theory of nanofiltration separation aiming at the technical bottleneck that a liquid-solid interface layer cannot be directly obtained and solute component concentration analysis is difficult to carry out. The invention innovatively provides a technical means for clarifying the solute distribution behavior of the liquid-solid two-phase interface layer, and visually analyzes the affinity of the components and the membrane material and the root cause of the pollution generated by the membrane according to the concentration distribution condition of the components of the interface layer; is helpful for researching and developing new membrane material, adjusting the concentration difference of different traditional Chinese medicine components distributed on the interface layer, amplifying the separation difference and carrying out targeted separation.

Description

Method for calculating concentration of traditional Chinese medicine components in interface layer of nanofiltration membrane
Technical Field
The invention relates to a method for calculating the concentration of a traditional Chinese medicine component in a nanofiltration membrane interface layer, in particular to a method for calculating the concentration of the component in the nanofiltration membrane interface layer based on a mode of realizing separation of the traditional Chinese medicine component by interface mass transfer, and belongs to the field of medicines.
Background
The existence of the interface is the root of all interface phenomena, so that the discussion of the equilibrium condition of the interface layer to the heterogeneous phase substances is carried out from Gibbs in the 19 th century, and the extensive attention of an analysis method and a theoretical model of a two-phase interface and the interface layer (interface region) is also attracted. At present, in the field of interface chemistry, three main recognitions are mainly provided for component concentration distribution of a two-phase interface layer, namely a Gibbs divided surface type interface layer model: it is believed that the energy, entropy, density, concentration, etc. of the two intersecting phases are uniform within each phase up to the physical interface when the two phases are brought into contact. Guggenheim transition layer type interface layer model: an interfacial layer is considered to be a thermodynamic entity having a certain volume and a certain content. And the distribution of material in the interfacial layer is not uniform. Double interface layer model: the two intersecting phases each have their own interface layer and the respective thermodynamic parameters are different, the concentration of the component in phase 1 changes as it transitions from the interior of phase 2 to the interface layer, but the concentration levels off as it approaches phase 2.
When different phases are contacted, a molecular heterogeneous distribution layer which is formed by two-phase molecular interpenetration exists at the contact position, wherein the evaluation of the physical and chemical properties of two-phase interface layers represented by alloys is relatively easy because the interface layers of the alloys are relatively fixed. The interface layer generated by the liquid-solid two phases is a thin layer of fluid formed around or on the solid relative to the flowing fluid, is a transition layer generated after the solution contacts with the solid, has physical and chemical properties different from those of the solution and the solid, has a thickness of between a few nanometers and a few micrometers, and cannot realize quantitative analysis of the component composition of the interface layer because the fluid of the interface layer is difficult to obtain directly.
In the nanofiltration separation process of traditional Chinese medicine components, the pore diameter of the nanofiltration membrane is distributed within the range of 100-1000 Da, the diameter is within the nanometer range, and the thickness of an interface layer generated after the nanofiltration membrane is contacted with the two phases is close to that of the interface layer, so that enough transmembrane pressure difference needs to be provided during nanofiltration separation to push the interface layer to enter the pore diameter of the membrane so as to realize separation. Therefore, the nanofiltration separation is a separation process realized on the basis of interface mass transfer, and theoretical support is provided for the calculation of the solute concentration of the liquid-solid two-phase interface layer on the basis of the separation process.
Based on the background, the invention provides a method for calculating the concentration of the traditional Chinese medicine components in the interface layer of the nanofiltration membrane, innovatively provides a technical means for clarifying the solute distribution behavior of the liquid-solid two-phase interface layer, and is beneficial to analyzing the tropism of the solute distribution after the two phases are contacted, so that the pollution mechanism of the nanofiltration membrane is clarified. The method is also helpful to the oriented separation of components in a complex solution environment by means of the distribution trend and the development of novel membrane materials.
Disclosure of Invention
The purpose of the invention is as follows: the invention aims to provide a method for calculating the concentration of traditional Chinese medicine components in an interface layer of a nanofiltration membrane, which can be used for analyzing the solute distribution characteristics of a liquid-solid two-phase interface layer.
The invention also aims to provide the application of the method for calculating the concentration of the traditional Chinese medicine components in the interface layer of the nanofiltration membrane in the aspects of analyzing the membrane pollution mechanism and the affinity of the components and the membrane material.
The technical scheme is as follows: a method for calculating the concentration of traditional Chinese medicine components in an interface layer of a nanofiltration membrane comprises the following steps: a. placing the nanofiltration membrane and Chinese medicinal components to be evaluated in a separation system, and detecting the concentration C of the Chinese medicinal components to be analyzed in the solution0(ii) a b. B, performing membrane separation on the traditional Chinese medicine solution in the step a, adjusting transmembrane pressure difference to be within the range of 0.05-2.0 MPa, and respectively collecting membrane flux (J) and component retention rate (R) data under corresponding transmembrane pressure difference; c. calculating the membrane separation coefficient k according to formula (1) from J and R under the series of transmembrane pressure differences in step b by pairing ln [ (1-R). J/R]And J linear regression, wherein
Figure BSA0000257095960000021
Is the intercept of a linear equation with a slope of 1/k
Figure BSA0000257095960000022
d. According to the separation coefficient k obtained by calculation in the step C, the concentration C of the traditional Chinese medicine component to be analyzed of the membrane interface layer is calculated according to the formula (2)m
Figure BSA0000257095960000023
The trans-membrane pressure difference in the step b needs to be selected from more than 3 trans-membrane pressure difference values.
And (c) calculating the component rejection rate (R) in the step (b) by using the method of R ═ trapped liquid solute concentration/raw liquid solute concentration.
And c, the regression coefficient of the linear equation in the step c is more than or equal to 0.99.
A method for calculating concentration of Chinese medicinal components in interface layer of nanofiltration membrane can be used for analyzing membrane pollution characteristics of Chinese medicinal components and affinity of Chinese medicinal components and membrane material.
A method for calculating concentration of Chinese medicinal components in interface layer of nanofiltration membrane is suitable for aqueous solution and organic solution.
Has the advantages that: (1) the technical bottleneck that the solute component concentration analysis is difficult to carry out due to the fact that the liquid-solid two-phase interface layer cannot be directly obtained is solved. (2) According to the concentration distribution of the interface layer components, the affinity of the components and the membrane material is visually analyzed, and the root cause of the pollution of the membrane is analyzed. (3) Is helpful for researching and developing new membrane material, adjusting the concentration difference of different traditional Chinese medicine components distributed on the interface layer, amplifying the separation difference and carrying out targeted separation.
Detailed Description
The foregoing aspects of the present invention are described in further detail below by way of examples, but it should not be construed that the scope of the above-described subject matter of the present invention is limited to the following examples, and that all the technologies implemented based on the above-described aspects of the present invention are within the scope of the present invention.
Example 1 concentration of chlorogenic acid in aqueous extract of honeysuckle in interfacial layer of composite polyamide nanofiltration membrane
Weighing the honeysuckle water extract, and detecting by adopting a high performance liquid chromatography, wherein the chromatographic conditions are as follows: waters e2695 HPLC, full-wavelength UV detector, Welch ultmate C18The method comprises the steps of carrying out chromatographic column chromatography, using acetonitrile-0.5% phosphoric acid (v/v 30: 70), carrying out volume flow rate of 1.0mL/min, carrying out column temperature of 30 ℃, carrying out detection wavelength of 340m, calculating the concentration of chlorogenic acid in a honeysuckle medicinal material water extract to be 112.02 mu g/mL by adopting an external standard point method, placing a 300Da composite polyamide nanofiltration membrane and the honeysuckle water extract in a separation system, adjusting transmembrane pressure differences to be 0.2, 0.5, 0.8, 1.0, 1.5 and 2.0MPa, and respectively collecting membrane flux (J) and component retention rate (R) data under the corresponding transmembrane pressure differences.
The membrane separation coefficient k was calculated according to equation (1) from J and R at a series of transmembrane pressure differences.
Figure BSA0000257095960000031
By the pair ln [ (1-R). J/R]And J linear regression, wherein
Figure BSA0000257095960000032
The slope is 1/k, the intercept of the linear equation. Fitting ln [ (1-R). J/R]And the linear equation for J is:
Figure BSA0000257095960000033
the regression coefficient was 0.9920.
Calculating the concentration C of chlorogenic acid in the film interface layer according to the formula (2) based on the calculated separation coefficient km。
Figure BSA0000257095960000034
CmAnd 115.02 mu g/mL indicates that the concentration of the chlorogenic acid in the interface layer is higher than the bulk concentration of the solution, and the chlorogenic acid is easy to approach the composite polyamide nanofiltration membrane.
Example 2 concentration of chlorogenic acid monomer compound in interfacial layer of composite polyamide nanofiltration membrane
Chlorogenic acid aqueous solution with the concentration of 112.0 mug/mL is prepared and is separated by a composite polyamide nanofiltration membrane of 300Da, transmembrane pressure differences are adjusted to be 0.2, 0.5, 0.8, 1.0, 1.5 and 2.0MPa, membrane fluxes (J) under the corresponding transmembrane pressure differences are respectively collected, and component rejection rate (R) data are measured and calculated by the liquid phase detection method in the embodiment 1.
The membrane separation coefficient k was calculated according to equation (1) from J and R at a series of transmembrane pressure differences.
Figure BSA0000257095960000035
By the pair ln [ (1-R). J/R]And J linear regression, wherein
Figure BSA0000257095960000036
The slope is 1/k, the intercept of the linear equation. Fitting ln [ (1-R). J/R]And the linear equation for J is:
Figure BSA0000257095960000037
the regression coefficient was 0.9908.
Calculating the concentration C of chlorogenic acid in the film interface layer according to the formula (2) based on the calculated separation coefficient km。
Figure BSA0000257095960000038
CmWhen the concentration of chlorogenic acid in the interface layer is higher than the bulk concentration of the solution in 143.02 μ g/mL, it can be seen that the concentration of chlorogenic acid of a single compound in the interface layer is higher than that of the honeysuckle extract in comparative example 1, which indicates that the other components in the honeysuckle extract are lower than that of chlorogenic acid and enter the interface layer to have a competitive effect.
Example 3 matrine and oxymatrine concentration in Sophora flavescens in nanofiltration Membrane interface layer
Weighing 1.0kg of radix Sophorae Flavescentis, extracting with 10 times of water for 2 times, each for 1 hr, mixing extractive solutions, filtering with microporous membrane to obtain radix Sophorae Flavescentis extractive solution, detecting by high performance liquid chromatography with the following chromatographic conditions: waters e2695 HPLC, full-wavelength UV detector, Agilent NH2Chromatographic column, acetonitrile-absolute ethyl alcohol-3% phosphoric acid (v/v/v 80: 10), volume flow rate of 1.0mL/min, column temperature of 25 deg.C, detection wavelength of 220nm, and external standard one-point method to calculate matrine concentration of 0.46 μ g/mL and oxymatrine concentration of 0.53 μ g/mL in water extract of radix Sophorae Flavescentis.
Separating with 500Da polyacrylonitrile nanofiltration membrane, adjusting transmembrane pressure difference to 0.2, 0.5, 0.8, 1.0, 1.5, and 2.0MPa, respectively collecting membrane flux (J) under corresponding transmembrane pressure difference, and calculating retention rate (R) data of matrine and oxymatrine.
The membrane separation coefficient k was calculated according to equation (1) from J and R at a series of transmembrane pressure differences.
Figure BSA0000257095960000041
By the pair ln [ (1-R). J/R]And J linear regression, wherein
Figure BSA0000257095960000042
The slope is 1/k, the intercept of the linear equation. Fitting ln [ (1-R). J/R]And the linear equation for J is:
matrine:
Figure BSA0000257095960000043
the regression coefficient was 0.9910.
Oxymatrine:
Figure BSA0000257095960000044
the regression coefficient was 0.9906.
Calculating the concentration C of matrine and oxymatrine at the membrane interface layer according to formula (2) based on the calculated separation coefficient km。
Figure BSA0000257095960000045
Matrine Cm0.41 mug/mL oxymatrine CmAnd in the aqueous extract of the radix sophorae flavescentis, the concentration of the matrine in the polyacrylonitrile nanofiltration membrane interface layer is higher than that of the oxymatrine, so that the matrine can easily enter the interface layer and permeate the nanofiltration membrane.
Example 4 concentration of matrine and oxymatrine in Sophora flavescens in nanofiltration membrane interface layer under ethanol solution environment
Weighing 1.0kg of radix Sophorae Flavescentis, extracting with 10 times of ethanol for 2 times, each for 1 hr, mixing extractive solutions, filtering with microporous membrane to obtain radix Sophorae Flavescentis ethanol extractive solution, detecting by high performance liquid chromatography with the following chromatographic conditions: waters e2695 HPLC, full-wavelength UV detector, Agilent NH2Chromatographic column, acetonitrile-absolute ethyl alcohol-3% phosphoric acid (v/v/v 80: 10), volume flow rate of 1.0mL/min, column temperature of 25 deg.C, detection wavelength of 220nm, and external standard one-point method for calculating matrine concentration of 0.54 μ g/mL in water extractive solution of radix Sophorae Flavescentis, and oxymatrineThe concentration of the base was 0.61. mu.g/mL.
Separating with 500Da polyacrylonitrile nanofiltration membrane, adjusting transmembrane pressure difference to 0.2, 0.5, 0.8, 1.0, 1.5, and 2.0MPa, respectively collecting membrane flux (J) under corresponding transmembrane pressure difference, and calculating retention rate (R) data of matrine and oxymatrine.
The membrane separation coefficient k was calculated according to equation (1) from J and R at a series of transmembrane pressure differences.
Figure BSA0000257095960000046
By the pair ln [ (1-R). J/R]And J linear regression, wherein
Figure BSA0000257095960000047
The slope is 1/k, the intercept of the linear equation. Fitting ln [ (1-R). J/R]And the linear equation for J is:
matrine:
Figure BSA0000257095960000048
the regression coefficient was 0.9908.
Oxymatrine:
Figure BSA0000257095960000049
the regression coefficient was 0.9924.
Calculating the concentration C of matrine and oxymatrine at the membrane interface layer according to formula (2) based on the calculated separation coefficient km。
Figure BSA0000257095960000051
Matrine Cm0.47 mug/mL oxymatrine Cm0.72 mu g/mL, compared with the aqueous solution environment, the k values of matrine and oxymatrine in the sophora flavescens ethanol extract are relatively reduced, the concentration of oxymatrine in the homopolymerization acrylonitrile nanofiltration membrane interface layer is obviously higher than that of matrine, which indicates that the oxymatrine can enter the interface layer formed by the ethanol solution environment and polyacrylonitrile more easilyAnd (4) a nanofiltration membrane is used.

Claims (6)

1. A method for calculating the concentration of traditional Chinese medicine components in an interface layer of a nanofiltration membrane is characterized by comprising the following steps:
a. placing the nanofiltration membrane and Chinese medicinal components to be evaluated in a separation system, and detecting the concentration C of the Chinese medicinal components to be analyzed in the solution0；
b. B, performing membrane separation on the traditional Chinese medicine solution in the step a, adjusting transmembrane pressure difference to be within the range of 0.05-2.0 MPa, and respectively collecting membrane flux (J) and component retention rate (R) data under corresponding transmembrane pressure difference;
c. calculating the membrane separation coefficient k according to formula (1) from J and R under the series of transmembrane pressure differences in step b by pairing ln [ (1-R). J/R]And J linear regression, wherein
Figure FSA0000257095950000011
Is the intercept of a linear equation with a slope of 1/k
Figure FSA0000257095950000012
d. According to the separation coefficient k obtained by calculation in the step C, the concentration C of the traditional Chinese medicine component to be analyzed of the membrane interface layer is calculated according to the formula (2)m
Figure FSA0000257095950000013
2. The method of claim 1, wherein more than 3 transmembrane pressure difference values are selected for the transmembrane pressure difference in step b.
3. The method of claim 1, wherein the component rejection rate (R) in step b is calculated as R-solute concentration of the retentate/solute concentration of the concentrate.
4. The method for calculating the concentration of the traditional Chinese medicine components in the nanofiltration membrane interface layer according to claim 1, wherein the regression coefficient of the linear equation in the step c is not less than 0.99.
5. The method of claim 1, wherein the method can be used to analyze the membrane contamination characteristics of herbal constituents and the affinity of herbal constituents with membrane materials.
6. The method of claim 1, wherein the method is suitable for aqueous solutions and organic solutions.
CN202111316854.7A 2021-11-08 2021-11-08 Method for calculating concentration of traditional Chinese medicine components in nanofiltration membrane interface layer Active CN114019054B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111316854.7A CN114019054B (en) 2021-11-08 2021-11-08 Method for calculating concentration of traditional Chinese medicine components in nanofiltration membrane interface layer

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111316854.7A CN114019054B (en) 2021-11-08 2021-11-08 Method for calculating concentration of traditional Chinese medicine components in nanofiltration membrane interface layer

Publications (2)

Publication Number Publication Date
CN114019054A true CN114019054A (en) 2022-02-08
CN114019054B CN114019054B (en) 2023-08-01

Family

ID=80062539

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202111316854.7A Active CN114019054B (en) 2021-11-08 2021-11-08 Method for calculating concentration of traditional Chinese medicine components in nanofiltration membrane interface layer

Country Status (1)

Country Link
CN (1) CN114019054B (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0882493A2 (en) * 1994-06-22 1998-12-09 Fls Miljo A/S Mass transfer method
US6044213A (en) * 1997-04-24 2000-03-28 Nec Corporation Process simulation method for calculating a surface oxidant concentration in oxidation process
US20070066928A1 (en) * 2005-09-22 2007-03-22 Jean-Michel Lannoy Automation and optimization of CRRT treatment using regional citrate anticoagulation
US20100250154A1 (en) * 2007-10-25 2010-09-30 Toray Industries, Inc Membranous filtration prediction method, prediction apparatus, and membranous filtration prediction program
CN102018835A (en) * 2009-09-18 2011-04-20 劲牌有限公司 Method for separating effective components in traditional Chinese medicine curculigo orchioides by membrane separation method

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0882493A2 (en) * 1994-06-22 1998-12-09 Fls Miljo A/S Mass transfer method
US6044213A (en) * 1997-04-24 2000-03-28 Nec Corporation Process simulation method for calculating a surface oxidant concentration in oxidation process
US20070066928A1 (en) * 2005-09-22 2007-03-22 Jean-Michel Lannoy Automation and optimization of CRRT treatment using regional citrate anticoagulation
US20100250154A1 (en) * 2007-10-25 2010-09-30 Toray Industries, Inc Membranous filtration prediction method, prediction apparatus, and membranous filtration prediction program
CN102018835A (en) * 2009-09-18 2011-04-20 劲牌有限公司 Method for separating effective components in traditional Chinese medicine curculigo orchioides by membrane separation method

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
JORGE EMILIO ALMAZÁN等: "Nanofiltration of glucose: Analysis of parametersand membrane characterization", CHEMICAL ENGINEERING RESEARCH AND DESIGN, pages 1 - 9 *
李存玉: "超微孔过滤原理的研究与应用", 中国博士学位论文全文数据库 工程科技I辑, no. 05, pages 016 - 1 *
李存玉等: "多指标评价有机相中纳滤分离丹酚酸B的适用性", 中草药, vol. 50, no. 6, pages 1328 - 1333 *

Also Published As

Publication number Publication date
CN114019054B (en) 2023-08-01

Similar Documents

Publication Publication Date Title
CN104817604B (en) A kind of purification process of β nicotinamide mononucleotides
Xu et al. The application of the modified PVDF ultrafiltration membranes in further purification of Ginkgo biloba extraction
Rao et al. Efficient recovery of the volatile aroma components from blackberry juice using a ZIF-8/PDMS hybrid membrane
CN104876993B (en) A kind of purification process of oxidized form β nicotinamide-adenine dinucleotide phosphates
CN109081775B (en) Directional separation and purification method of diaryl heptane compounds in saxifraga tangutica
US20200010618A1 (en) A process for purification of polyether block copolymers
CN206315290U (en) A kind of Chinese medicine extract isolates and purifies system
CN104876994A (en) Method for purifying oxidized beta-nicotinamide adenine dinucleotide
CN101669980A (en) Method for extracting and separating icariin and flavone from epimedium herb
CN102070688A (en) Method for enriching and purifying icariin in epimedium herb
CN114019054A (en) Method for calculating concentration of traditional Chinese medicine components in interface layer of nanofiltration membrane
CN109081858B (en) Directional separation and purification method of flavonoid compounds in saxifrage tangutica
CN114988979B (en) Method for preparing high-purity lycopene by macro separation
CN102757377B (en) 5-hydroxytryptophan purification method as well as obtained 5-hydroxytryptophan drug
CN107936033A (en) The method that industrially prepared liquid chromatogram isolates and purifies koumine
CN104926659B (en) The method preparing rosmarinic acid chemical reference substance from three kinds of high mountain claries
CN103145729B (en) Bilobalide B compound and preparation method thereof
CN104496815B (en) A kind of preparation method of medicinal chlorogenic acid
CN105111326B (en) A kind of preparation method and applications of Polysaccharide in Pleurotus eryngii component
CN101628930A (en) Method for separating polypeptide with tris ligand and agarose medium
Li et al. Effect of pH on microfiltration of Chinese herb aqueous extract by zirconia membrane
CN103265602A (en) Method for separating macranthoidin from lonicerae flos
CN101747391B (en) Method for separating and preparing chemical reference substance of Icariin
CN106916863A (en) A kind of method of κ carrageenan oligosaccharides monomer fast separating and purifying
CN104130127A (en) Process method for extracting chlorogenic acid from blumea riparia(BL.)DC

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant