CN114018787A - Particle detection unit, mixing system and mixing method - Google Patents
Particle detection unit, mixing system and mixing method Download PDFInfo
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- CN114018787A CN114018787A CN202111236637.7A CN202111236637A CN114018787A CN 114018787 A CN114018787 A CN 114018787A CN 202111236637 A CN202111236637 A CN 202111236637A CN 114018787 A CN114018787 A CN 114018787A
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- 238000001514 detection method Methods 0.000 title claims abstract description 84
- 238000002156 mixing Methods 0.000 title claims abstract description 69
- 239000002245 particle Substances 0.000 title claims abstract description 48
- 238000000034 method Methods 0.000 title claims abstract description 27
- 239000000758 substrate Substances 0.000 claims abstract description 60
- 239000000463 material Substances 0.000 claims abstract description 18
- 238000007664 blowing Methods 0.000 claims description 138
- 238000002347 injection Methods 0.000 claims description 87
- 239000007924 injection Substances 0.000 claims description 87
- 239000007788 liquid Substances 0.000 claims description 50
- 239000012491 analyte Substances 0.000 claims description 34
- 239000000126 substance Substances 0.000 claims description 23
- 238000007789 sealing Methods 0.000 claims description 14
- 238000004891 communication Methods 0.000 claims description 7
- 238000011896 sensitive detection Methods 0.000 claims description 3
- 230000004907 flux Effects 0.000 claims description 2
- 238000005070 sampling Methods 0.000 claims 2
- 230000000740 bleeding effect Effects 0.000 abstract description 2
- 239000008187 granular material Substances 0.000 abstract description 2
- 238000004043 dyeing Methods 0.000 description 12
- 238000005086 pumping Methods 0.000 description 8
- 230000008569 process Effects 0.000 description 5
- 239000000203 mixture Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000003780 insertion Methods 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- 238000007689 inspection Methods 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 2
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- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 2
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- 239000004793 Polystyrene Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000002355 dual-layer Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
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- 238000012986 modification Methods 0.000 description 1
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- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
- G01N2001/386—Other diluting or mixing processes
- G01N2001/387—Other diluting or mixing processes mixing by blowing a gas, bubbling
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
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Abstract
The invention relates to a particle detection unit, a mixing system and a mixing method, wherein the particle detection unit comprises: the device comprises a substrate, a sample introduction device, a flow channel and a suction device, wherein the sample introduction device and the suction device are arranged on the substrate; one end of the flow channel is communicated with the sample feeding device through the outlet; the suction device is connected with the sample feeding device through the flow channel. The user does not need to mix the sample manually; can arrange the colour material in the granule detecting element and be located the route before the detection window, the sample is by the suction subassembly through the suction device in-process that flows of bleeding, can directly mix with the colour material, removes the step of artifical mixed colour material from to improve detection efficiency greatly.
Description
Technical Field
The invention relates to the technical field of cell detection, in particular to a particle detection unit, a mixing system and a mixing method.
Background
In the field of biotechnology, cell detection is usually carried out by adopting card type consumables, manual sample loading is needed on the consumables, and samples need to be fully mixed manually before sample loading; the multi-channel consumable needs to prepare color substances by self, manually mixes sample liquid and liquid color substances, then manually injects the mixture into a detection window, and then observes and counts the mixture under a fluorescence microscope; therefore, the cell detection efficiency is low.
Disclosure of Invention
In view of the above, it is desirable to provide a particle detection unit, a mixing system and a mixing method that can improve detection efficiency.
A particle detection unit, the particle detection unit comprising:
a substrate;
the sample introduction device is arranged on the substrate and provided with a sample introduction port, and an outlet;
the flow channel is embedded in the substrate, and one end of the flow channel is communicated with the sample injection device through the outlet; and
and the suction device is arranged on the substrate and is connected with the sample injection device through the flow channel.
In one embodiment, the flow channel may allow at least a portion of the liquid analyte to flow within the channel, the flow channel having at least a portion with a bend or obstruction such that the liquid analyte at the bend or obstruction acts to adjust the liquid analyte flow rate to lengthen the flow path.
In one embodiment, the particle detection unit further comprises a blowing and sucking device, the blowing and sucking device is arranged on the substrate and provided with a blowing and sucking mixing port, and the sample injection device is provided with a sample injection mixing port spaced from the outlet; and one end of one of the flow channels is communicated with the blowing and sucking device through the blowing and sucking uniform mixing port, and the other end of the flow channel is communicated with the sample injection device through the sample injection uniform mixing port.
In one embodiment, the flow channel has at least two branches which are connected to and disconnected from the suction device by valve control.
In one embodiment, the substrate is a sheet material, and the substrate may surround at least a portion of the flow channel.
In one embodiment, the flow channels have a channel flux equivalent diameter in the range of 10-1000 μm.
In one embodiment, the suction device comprises a suction cavity arranged on the substrate and a sealing plug arranged on the suction cavity, and the suction cavity is communicated with the flow channel; the sealing plug is used for connecting with a suction assembly for providing suction power.
In one embodiment, the particle detection unit further includes a detection window disposed on the substrate, one end of the detection window is communicated with the flow channel, and the other end of the detection window is communicated with the suction device.
In one embodiment, the other end of the detection window is communicated with the suction device through a connecting channel.
In one embodiment, a moisture-sensitive detection member is disposed in the connecting passage.
In one embodiment, a filter is disposed within the suction device.
In one embodiment, the particle detection unit further comprises a color material, the color material is disposed in the flow channel, or disposed in the sample injection device, or disposed in the suction device, or disposed in the blowing and sucking device, and the color material is used for mixing with the liquid analyte injected into the sample injection device.
A mixing system, comprising:
the particle detection unit; and
the suction assembly comprises a suction power source, an aspirator and a suction needle device, wherein the suction needle device is used for being inserted into the suction device, the suction needle device is communicated with the aspirator, and the suction power source is used for driving the aspirator to act so as to suck air to the suction device through the suction needle device.
In one embodiment, the suction power source is a first linear stepper motor, the aspirator includes a first cylinder and a first piston rod, one end of the first piston rod is disposed in the first cylinder, the other end of the first piston rod is disposed at the output end of the first linear stepper motor, and the suction needle device is communicated with the first cylinder.
In one embodiment, the mixing system further includes a blowing and sucking component, the blowing and sucking component includes a blowing and sucking power source, a blowing and sucking device, and a blowing and sucking needle device, the blowing and sucking needle device is used for being inserted into the blowing and sucking device, the blowing and sucking needle device is communicated with the blowing and sucking device, and the blowing and sucking power source is used for driving the blowing and sucking device to move so as to blow or suck air to the blowing and sucking device through the blowing and sucking needle device.
In one embodiment, the blowing and sucking power source is a second linear stepping motor, the blowing and sucking device comprises a second cylinder and a second piston rod, one end of the second piston rod is arranged in the second cylinder, and the other end of the second piston rod is arranged at the output end of the second linear stepping motor; the blow and suction needle device is communicated with the second cylinder body.
In one embodiment, the mixing system further comprises a third power source and a driving plate, the suction needle means and the blow needle means are disposed on the driving plate, and the third power source is used for driving the driving plate to move in a direction approaching or separating from the base plate.
A method of mixing, comprising the steps of:
injecting a liquid analyte into a sample injection device through a sample injection port of the sample injection device, wherein the sample injection device is arranged on a substrate, a flow channel is arranged in the substrate, and a suction device communicated with the sample injection device through the flow channel is also arranged on the substrate;
inserting a suction needle device into the suction apparatus;
the suction power source drives the suction device to act so as to suck the suction device through the suction needle device, and the liquid analyte in the sample feeding device enters the flow channel.
A method of mixing comprising the steps of:
injecting a liquid analyte into a sample injection device through a sample injection port of the sample injection device, wherein the sample injection device is arranged on a substrate, a flow channel is arranged in the substrate, and a suction device and a blowing-suction device which are respectively communicated with the sample injection device through the flow channel are also arranged on the substrate;
inserting a blowing and sucking needle-shaped device into a blowing and sucking device;
the blowing and sucking device is driven to act by the blowing and sucking power source so as to blow or exhaust air to the blowing and sucking device through the blowing and sucking needle-shaped device, so that the liquid analyte in the sample injection device flows to the inside of the blowing and sucking device through the flow channel or the liquid analyte in the blowing and sucking device flows to the inside of the sample injection device through the flow channel, and the liquid analytes are uniformly mixed;
inserting a suction needle device into the suction apparatus;
the suction power source drives the suction device to act so as to suck the suction device through the suction needle-shaped device, and the uniformly mixed liquid analyte in the sample introduction device enters the flow channel.
In one embodiment, the mixing method further includes the following steps:
a color substance is disposed in the flow channel, the sample introduction device, the aspiration device, or the blow-and-suck device, the color substance being mixed with the liquid analyte.
The particle detection unit, the mixing system and the mixing method have at least the following advantages:
adding a sample into the sample introduction device through the sample introduction port, inserting the suction needle-shaped device of the suction assembly into the suction device, pumping the suction device by a suction power source of the suction assembly through the suction needle-shaped device, pumping the sample in the sample introduction device into the flow channel of the substrate through the outlet, blowing air to the suction device by the suction power source of the suction assembly through the suction needle-shaped device, reversely blowing the sample in the flow channel into the sample introduction device, and repeatedly pumping and blowing the sample for multiple times to realize full mixing; in addition, the suction power source of the suction assembly sucks air from the suction device through the suction needle device, and the sample in the sample injection device is sucked into the flow channel of the substrate through the outlet and finally flows to the detection window for detection. Therefore, the sample is directly injected into the sample injection device, and a user does not need to manually mix the sample; also can arrange the colour material in the granule detecting element and be located the route before the detection window, the sample is by the suction module through the suction device flow in-process of bleeding, can directly mix with the colour material, removes the step of artifical mixed colour material from to improve detection efficiency greatly.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the invention and, together with the description, serve to explain the invention and not to limit the invention.
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
FIG. 1 is a schematic diagram of a mixing system in one embodiment;
FIG. 2 is a top view of a sample plate in one embodiment;
FIG. 3 is a top view of the particle detection unit of the sample plate of FIG. 2;
FIG. 4 is a side view of a particle detection unit of the sample plate shown in FIG. 2;
FIG. 5 is a block flow diagram of a hybrid method in one embodiment;
fig. 6 is a flow chart diagram of a mixing method in another embodiment.
Description of reference numerals:
10. a mixing system; 100. a sample plate; 200. a suction assembly; 300. a blowing and sucking component; 400. a particle detection unit; 410. a substrate; 420. a sample introduction device; 430. a blowing and sucking device; 440. a second microchannel; 450. a first microchannel; 460. detecting a window; 470. a suction device; 421. a sample inlet; 422. an outlet; 431. a blowing and sucking mixing port; 423. a sample injection and mixing port; 480. a connecting channel; 490. a filter; 481. a moisture sensitive detection member; 210. a suction power source; 220. an aspirator; 230. aspirating the needle-shaped device; 471. a first sealing plug; 310. a blowing and sucking power source; 320. a blowing and sucking device; 330. blowing and sucking a needle-shaped device; 432. a second sealing plug; 500. a third power source; 600. a drive plate.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.
Referring to fig. 1 and 2, one embodiment of a mixing system 10 includes a particle detection unit 400 and a pumping assembly 200. The suction unit 200 mixes or stains the sample injected into the particle detection unit 400 by sucking the sample plate 100.
Specifically, the sample plate 100 includes a plurality of particle detecting units 400. For example, referring to fig. 2, the sample plate 100 includes 24 particle detecting units 400, and the 24 particle detecting units 400 are distributed in 2 rows and 12 rows to form a 24-channel sample plate 100, so that the 24 particle detecting units 400 can be sequentially detected, thereby improving the working efficiency. Of course, in other embodiments, the sample plate 100 may further include other numbers of particle detecting units 400, such as 2, 3, 10, 20, etc., and the number and arrangement positions of the particle detecting units 400 may be set according to actual requirements.
Referring to fig. 3 and 4, the particle detecting unit 400 includes a substrate 410, a sample injection device 420, a flow channel, and a pumping device 470. Further, the particle detecting unit 400 further includes a detecting window 460 and a blowing and sucking device 430. The substrate 410 may be made of Polystyrene (PS), Polycarbonate (PC), or polymethyl methacrylate (PMMA). The base plates 410 of all the particle detecting units 400 on the sample plate 100 are integrally formed.
In one embodiment, the sample injection device 420 is disposed on the substrate 410, and the top of the sample injection device 420 is provided with a sample injection port 421. Specifically, the top of the sample injection device 420 is opened to form a sample injection port 421, so that the convenience of sample injection is improved. Of course, in other embodiments, the sample inlet 421 may be opened at the top of the sample injection device 420, and the size of the sample inlet 421 is smaller than the inner diameter size of the top of the sample injection device 420. The sample introduction device 420 is provided with an outlet 422. Specifically, the outlet 422 is opened on the bottom surface of the sample introduction device 420. Of course, in other embodiments, the outlet 422 may also be opened on the side of the bottom of the sample introduction device 420. Alternatively, in another embodiment, the outlet 422 may be opened on the side of the middle portion of the sample injection device 420, as long as the sample in the sample injection device 420 can be smoothly introduced into the flow channel. In the present embodiment, the number of the outlets 422 is one. Of course, in other embodiments, the number of outlets 422 may be as large.
Optionally, the flow channel includes a first fluidic channel 450 and a second fluidic channel 440. The first microchannel 450 is not in direct communication with the second microchannel 440. For example, in fig. 4, the first fluidic channel 450 is located below the second fluidic channel 440, and both the first fluidic channel 450 and the second fluidic channel 440 form a dual-layer channel structure.
Specifically, the first micro flow channel 450 is embedded in the substrate 410, and one end of the first micro flow channel 450 is communicated with the sample injection device 420 through the outlet 422. The first micro flow channel 450 is embedded with a color material. Specifically, the diameter of the first microchannel 450 may range from 10 microns to 1000 microns. The first micro flow channel 450 can be formed on the substrate 410 by micro flow channel technology, and embedded in the substrate 410 to prevent the first micro flow channel 450 from being damaged by exposure. The color substance in the first micro flow channel 450 is sprayed in the first micro flow channel 450 by spraying, and the color substance is used for dyeing the sample.
Further, the first micro flow channel 450 is bent to extend the dyeing path, thereby improving the dyeing effect. For example, in fig. 3, the first microchannel 450 includes a plurality of straight segments, and the straight segments are bent and connected to form the first microchannel 450. Of course, in other embodiments, the first microchannel 450 may also be an arc segment or the like.
Further, a detection window 460 is disposed on the substrate 410, and one end of the detection window 460 is communicated with the first micro flow channel 450. The detection window 460 is mainly used for the camera to take pictures, so the detection window 460 is transparent.
Further, a suction device 470 is disposed on the substrate 410, and the other end of the inspection window 460 is communicated with the suction device 470. For example, the other end of the detection window 460 is in communication with the suction device 470 through a connection passage 480. The diameter of the connecting channel 480 may also range from 10 microns to 1000 microns.
Optionally, a filter 490 is provided within the suction device 470. The filter 490 is provided with a hydrophobic air-permeable material, and when the liquid in the first microchannel 450 reaches the filter 490, the filter 490 keeps the liquid in the entire first microchannel 450 in a non-flowing state or a slow-flowing state, so as to ensure that the liquid in the detection window 460 does not flow or flows slowly, thereby facilitating observation.
Further, a humidity-sensitive detecting member 481 is provided in the connecting channel 480, and when the liquid in the first micro flow channel 450 passes through the humidity-sensitive detecting member 481, the humidity-sensitive detecting member 481 changes color, thereby proving that the particle detecting unit 400 has been used. For example, the wetness sensitive detecting member 481 may be a wetness sensitive detecting paper.
Optionally, the mixing system 10 further comprises a blowing and sucking assembly 300, and the blowing and sucking assembly 300 mixes the sample in the particle detection unit 400 by pumping or blowing. The blowing and sucking device 430 is arranged on the substrate 410, and a blowing and sucking uniformly mixing port 431 is arranged at the bottom of the blowing and sucking device 430. Specifically, the mixing and blowing port 431 is disposed at the side of the bottom of the blowing and sucking device 430. Of course, in other embodiments, the mixing port 431 may be disposed on the bottom surface of the mixing device 430. The bottom of the sample injection device 420 is also provided with a sample injection and mixing port 423 which is mutually spaced with the outlet 422. Similarly, the sample blending port 423 is disposed on a side surface of the bottom of the sample injection device 420, and the blow-suction blending port 431 is disposed opposite to the sample blending port 423. One end of the second micro-channel 440 is communicated with the blowing and sucking device 430 through the blowing and sucking port 431, and the other end is communicated with the sample injection device 420 through the sample injection port 423. The blowing and sucking device 430 and the sample injection device 420 may be arranged close to each other or at intervals, as long as the blowing and sucking device 430 is ensured to be communicated with the sample injection device 420 through the second microchannel 440. The blowing and sucking device 430 is positioned between the sample injection device 420 and the suction device 470, so that the reasonability of arrangement is improved, and the space is saved.
Further, the diameter of the second micro flow channel 440 may range from 10 microns to 1000 microns. The second micro flow channels 440 may be formed on the substrate 410 by micro flow channel technology. For example, the second micro-fluidic channel 440 can be embedded in the substrate 410 to prevent the micro-fluidic channel from being damaged by exposure.
Referring again to fig. 1, the suction assembly 200 includes a suction power source 210, a suction unit 220, and a suction needle 230, and the suction needle 230 is inserted into the suction unit 470. For example, the suction device 470 includes a suction cavity disposed on the substrate 410 and a first sealing plug 471 disposed on the suction cavity. The top of the suction device 470 is provided with a first sealing plug 471 for insertion of the suction needle means 230. The aspiration needle device 230 is a steel needle. The first sealing plug 471 may be a silicone plunger or a soft sealing glue, which facilitates the insertion of the suction needle 230 into the suction device 470 for air suction. The suction needle 230 is communicated with the suction unit 220, and the suction power source 210 is used for driving the suction unit 220 to suck air from the suction unit 470 through the suction needle 230, so that the suction needle 230 is not in contact with the liquid in the suction unit 470, the suction needle 230 is not contaminated, cleaning is not required, and cost is reduced.
The sample is added into the sample introduction device 420 through the sample introduction port 421, the suction needle 230 of the suction assembly 200 is inserted into the suction device 470, the suction power source 210 is used to drive the suction device 220 to suck air to the suction device 470 through the suction needle 230, the sample in the sample introduction device 420 is drawn into the first microchannel 450 through the outlet 422, since the first micro flow channel 450 is filled with the color material, the sample is stained in the first micro flow channel 450 and flows into the detection window 460 by the pumping action of the aspirator 220, and finally reaches the filter 490, after the liquid in the first microchannel 450 reaches the filter 490, the liquid in the entire first microchannel 450 is kept in a non-flowing state or a slow-flowing state by the filter 490, so as to ensure that the liquid in the detection window 460 does not flow any more or flows slowly, thereby facilitating observation and counting of the detection window 460 through a microscope.
Specifically, the suction power source 210 may be a first linear stepping motor, the aspirator 220 includes a first cylinder and a first piston rod, one end of the first piston rod is disposed in the first cylinder, the other end of the first piston rod is disposed at the output end of the first linear stepping motor, and the suction needle device 230 is communicated with the first cylinder. For example, the first linear stepping motor rotates forward to drive the first piston rod to extend out of the first cylinder, and the suction device 220 sucks air from the suction device 470. The first linear stepping motor rotates reversely to drive the first piston rod to retract into the first cylinder, and the suction device 220 does not suck air from the suction device 470.
Specifically, the blowing and sucking assembly 300 includes a blowing and sucking power source 310, a blowing and sucking device 320, and a blowing and sucking needle device 330, wherein the blowing and sucking needle device 330 is inserted into a blowing and sucking device 430. For example, the blowing and sucking device 430 includes a blowing and sucking cavity disposed on the substrate 410 and a second sealing plug 432 disposed on the blowing and sucking cavity. The top of the blow and suction device 430 is provided with a second sealing plug 432 into which the blow and suction needle means 330 is inserted. The second sealing plug 432 may be a silica gel plunger or a soft sealing rubber, which facilitates the insertion of the blowing and sucking needle device 330 into the blowing and sucking device 430 for blowing or sucking air. The blowing and sucking needle device 330 is communicated with the blowing and sucking device 320, and the blowing and sucking power source 310 is used for driving the blowing and sucking device 320 to operate so as to blow or suck air to the blowing and sucking device 430 through the blowing and sucking needle device 330, so that the blowing and sucking needle device 330 does not contact with a sample in the blowing and sucking device 430, no pollution is generated, cleaning is not needed, and the cost is reduced.
Specifically, the blowing and sucking power source 310 is a second linear stepping motor, the blowing and sucking device 320 includes a second cylinder and a second piston rod, one end of the second piston rod is disposed in the second cylinder, and the other end of the second piston rod is disposed at an output end of the second linear stepping motor. The blowing and sucking needle device 330 is communicated with the second cylinder, for example, the second linear stepping motor rotates forward to drive the second piston rod to extend out of the second cylinder, at this time, the blowing and sucking device 320 performs air suction on the blowing and sucking device 430, and the sample in the sample injection device 420 flows into the blowing and sucking device 430 through the second microchannel 440. The second linear stepping motor rotates reversely to drive the second piston rod to retract into the second cylinder, at this time, the blowing and sucking device 320 blows air into the blowing and sucking device 430, and the sample in the blowing and sucking device 430 flows into the sample introduction device 420 through the second microchannel 440. The second piston rod is driven to extend out or retract into the second cylinder body through the positive rotation and the reverse rotation of the second linear stepping motor, so that the purpose of pumping or blowing air to the blowing and sucking device 430 is achieved, the sample injected into the sample injection device 420 is blown back and forth and uniformly mixed, the sample is prevented from being precipitated, the detected cell concentration is ensured, and the detection precision is improved.
Further, referring again to fig. 1, the mixing system 10 further includes a third power source 500 and a driving plate 600, the suction pin means 230 and the blow pin means 330 are disposed on the driving plate 600, and the third power source 500 is used for driving the driving plate 600 to move in a direction approaching or separating from the base plate 410. The third power source 500 may be a motor, and the driving plate 600 is driven to descend by the forward rotation of the motor, the suction pin 230 is inserted into the suction device 470, and the blow-suction pin 330 is inserted into the blow-suction device 430.
In other embodiments, the blowing and sucking assembly 300 and the blowing and sucking device 430 may not be provided, and the mixing or dyeing of the sample in the particle detection unit 400 may be realized by the sucking assembly 200 and the sucking device 470. Optionally, the flow channel has at least two branches that are controlled by valves to communicate with and disconnect from the suction device 470. The valve of one branch is opened, the suction assembly 200 sucks air or blows air, and the sample flows back and forth between the suction device 470 and the sample injection device 420 through the branch to realize uniform mixing; after mixing, the branch is closed, the valve of the other branch is opened, the color material can be buried in the other branch, the mixed sample is pumped through the suction assembly 200, enters the other branch, is dyed and flows to the detection window 460 for detection. Alternatively, the flow channel is a single flow channel, and air is sucked by the suction assembly 200, so that the sample flows between the suction device 470 and the sample injection device 420 through the flow channel to achieve uniformly mixed mixing or dyeing. In one embodiment, the flow channel may allow at least a portion of the liquid analyte to flow within the channel, the flow channel having at least a portion with a bend or obstruction such that the liquid analyte at the bend or obstruction acts to adjust the liquid analyte flow rate to lengthen the flow path.
Optionally, the particle detecting unit 400 further comprises a color material disposed in the flow channel, or disposed in the sample injection device 420, or disposed in the suction device 470, or disposed in the blowing and sucking device 430, the color material being used for mixing with the liquid analyte injected into the sample injection device 420. The sample is a liquid analyte.
Adding a sample into the sample introduction device 420 through the sample introduction port 421, inserting the suction needle 230 of the suction assembly 200 into the suction device 470, sucking the suction device 470 by the suction power source 210 of the suction assembly 200 through the suction needle 230, sucking the sample in the sample introduction device 420 into the flow channel of the substrate 410 through the outlet 422, blowing the suction device 470 by the suction power source 210 of the suction assembly 200 through the suction needle 230, blowing the sample in the flow channel reversely into the sample introduction device 420, and repeatedly sucking and blowing the sample for multiple times to realize sufficient mixing; in addition, the suction power source 210 of the suction assembly 200 sucks the suction device 470 through the suction needle 230, and the sample in the sample introduction device 420 is sucked into the flow channel of the substrate 410 through the outlet 422 and finally flows to the detection window 460 for detection. Therefore, the sample is directly injected into the sample injection device 420, and the user does not need to mix the sample manually; the color substance can also be placed in the particle detection unit 400 in the path before the detection window 460, and the sample can be directly mixed with the color substance in the process of air suction flowing from the suction assembly 200 through the suction device 470, so that the step of manually mixing the color substance is omitted, and the detection efficiency is greatly improved.
Referring to fig. 5, an embodiment of the present application provides a mixing method, including the steps of:
step S110, injecting the liquid analyte into the sample injection device 420 through the sample injection port 421 of the sample injection device 420, wherein the sample injection device 420 is disposed on the substrate 410, a flow channel is disposed in the substrate 410, and the substrate 410 is further provided with a suction device 470 communicated with the sample injection device 420 through the flow channel.
In step S120, the suction needle device 230 is inserted into the suction apparatus 470. For example, the suction needle means 230 may be driven by the third power source 500 and the driving plate 600 to move in a direction to approach the base plate 410 until the suction needle means 230 is inserted into the blow-suction device 430.
In step S130, the suction power source 210 drives the suction unit 220 to suck the suction needle 230 to the suction device 470, so that the liquid analyte in the sample introduction device 420 enters the flow channel.
The mixing method can be implemented by using the mixing system 10 of any of the above embodiments, the suction power source 210 of the suction module 200 sucks or blows air to the suction device 470 through the suction needle 230, the sample in the sample introduction device 420 is sucked into the flow channel of the substrate 410 through the outlet 422 or the sample in the flow channel is blown back into the sample introduction device 420, and the sample can be fully mixed through repeated movements. The suction power source 210 of the suction assembly 200 sucks air from the suction device 470 through the suction needle 230, and the sample in the sample injection device 420 is sucked into the flow channel of the substrate 410 through the outlet 422 and finally flows to the inspection window 460 for inspection. The user does not need to mix the sample manually; the color substance can also be placed in the particle detection unit 400 in the path before the detection window 460, and the sample can be directly mixed with the color substance in the process of air suction flowing from the suction assembly 200 through the suction device 470, so that the step of manually mixing the color substance is omitted, and the detection efficiency is greatly improved.
Referring to fig. 6, an embodiment of the present application further provides a mixing method, including the following steps:
step S210, injecting the liquid analyte into the sample injection device 420 through the sample injection port 421 of the sample injection device 420, wherein the sample injection device 420 is disposed on the substrate 410, a flow channel is disposed in the substrate 410, and the substrate 410 is further provided with a suction device 470 and a blow-and-suck device 430 respectively communicated with the sample injection device 420 through the flow channel.
In step S220, the blow-suction needle 330 is inserted into the blow-suction device 430.
In step S230, the blowing and sucking power source 310 drives the blowing and sucking device 320 to operate to blow or suck air into the blowing and sucking device 430 through the blowing and sucking needle 330, so that the liquid analyte in the sample injection device 420 flows into the blowing and sucking device 430 through the flow channel or the liquid analyte in the blowing and sucking device 430 flows into the sample injection device 420 through the flow channel, so as to mix the liquid analytes uniformly.
In step S240, the suction needle device 230 is inserted into the suction device 470. Step S240 and step S220 may be performed sequentially or simultaneously.
In step S250, the suction power source 210 drives the suction unit 220 to perform suction on the suction device 470 via the suction needle 230, so that the uniformly mixed liquid analyte in the sample introduction device 420 enters the flow channel.
The mixing method can be implemented by using the mixing system 10 in any of the above embodiments, the blowing and sucking power source 310 of the blowing and sucking assembly 300 evacuates or blows air to the blowing and sucking device 430 through the blowing and sucking needle device 330, the sample in the sample injection device 420 is drawn into the flow channel of the substrate 410 or the sample in the flow channel is blown reversely into the sample injection device 420, and the sample can be fully mixed after being repeatedly activated for many times; the suction power source 210 of the suction assembly 200 sucks air from the suction device 470 through the suction needle 230, and the mixed sample in the sample injection device 420 is sucked into the flow channel of the substrate 410 and finally flows to the detection window 460 for detection. The user need not manual mixed sample to improve detection efficiency greatly, pressure-vaccum device 430 bleeds or blows, makes the sample of injecting into sample introduction device 420 and makes a round trip to blow and beat the mixing, prevents that the sample from deposiing, ensures the cell concentration that detects, improves the precision that detects.
Further, in one embodiment, the mixing method further includes the following steps:
step S260, a color substance is disposed in the flow channel, the sample introduction device 420, the suction device 470 or the blowing and sucking device 430, and the color substance is mixed with the liquid analyte.
The color substance is placed in the particle detection unit 400 and positioned in the path before the detection window 460, and the sample is directly mixed with the color substance in the process of air suction flow by the suction assembly 200 through the suction device 470 or air suction and air blowing flow by the blow-suction assembly 300 through the blow-suction device 430, so that the step of manually mixing the color substance is omitted, the dyeing efficiency is greatly improved, and the detection efficiency is further improved.
The specific working principle of the hybrid system 10 and the hybrid method thereof is as follows:
the sample is injected into the sample injection device 420 through the sample injection port 421, and in order to ensure the cell concentration to be detected, the sample needs to be uniformly mixed before the dyeing detection, so as to prevent the cell from settling. The third power source 500 drives the driving plate 600 to descend, so that the suction needle means 230 is inserted into the suction unit 470 and the blow-suction needle means 330 is inserted into the blow-suction unit 430.
The second linear stepping motor rotates forward to drive the second piston rod to extend out of the second cylinder, at this time, the blowing and sucking device 320 performs air suction on the inside of the blowing and sucking device 430 through the blowing and sucking needle device 330, and the sample in the sample introduction device 420 flows into the blowing and sucking device 430 through the second microchannel 440. And then the second linear stepping motor rotates reversely to drive the second piston rod to retract into the second cylinder, at this time, the blowing and sucking device 320 blows air to the blowing and sucking device 430 through the blowing and sucking needle device 330, and the sample in the blowing and sucking device 430 flows into the sample introduction device 420 through the second microchannel 440. At least three rounds of suction and aspiration are performed to mix the sample.
After the sample is uniformly mixed, the dyeing operation is started, the first linear stepping motor rotates forwards to drive the first piston rod to extend out of the first cylinder body, at the moment, the aspirator 220 sucks air in the suction device 470 through the suction needle device 230, the sample is sucked into the first micro flow channel 450 through the outlet 422, the sample dyeing is realized in the flowing process of the first micro flow channel 450, and finally the sample flows into the detection window 460 and finally reaches the filter 490. Because the filter 490 is made of a hydrophobic and air permeable material, when the liquid in the first microchannel 450 reaches the filter 490, the liquid in the entire first microchannel 450 is kept in a non-flowing state or a slow-flowing state by the filter 490, so as to ensure that the liquid in the detection window 460 does not flow or flows slowly, which is convenient for observation.
Therefore, the sample can be directly injected into the sample injection device 420 without manual dyeing of a user, the injection amount of the sample has no influence on the accuracy of the result, the color substances are embedded in the first micro-channel 450 in advance, the step of manually preparing the color substances is omitted, the consistency of the dyeing concentration is ensured, the accuracy is improved, and the full automation and more accurate result of the detection process are ensured through the sample injection amount accurately controlled by the suction power source 210 and the aspirator 220 without being interfered by manual operation. Moreover, the sample is uniformly mixed in a blowing and air suction mode before the dyeing operation, so that the cell sedimentation is prevented, the detected cell concentration is ensured, and the detection precision is improved.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.
It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.
Claims (20)
1. A particle detection unit, characterized in that the particle detection unit comprises:
a substrate;
the sample introduction device is arranged on the substrate and provided with a sample introduction port, and an outlet;
the flow channel is embedded in the substrate, and one end of the flow channel is communicated with the sample injection device through the outlet; and
and the suction device is arranged on the substrate and is connected with the sample injection device through the flow channel.
2. The particle detection unit of claim 1, wherein the flow channel is configured to allow at least a portion of the liquid analyte to flow within the channel, the at least a portion of the flow channel having a bend or obstruction such that the liquid analyte at the bend or obstruction acts to adjust a liquid analyte flow rate to lengthen the flow path.
3. The particle detection unit of claim 2, further comprising a blowing and sucking device, wherein the blowing and sucking device is arranged on the substrate, the blowing and sucking device is provided with a blowing and sucking mixing port, and the sampling device is provided with a sampling mixing port spaced from the outlet; and one end of one of the flow channels is communicated with the blowing and sucking device through the blowing and sucking uniform mixing port, and the other end of the flow channel is communicated with the sample injection device through the sample injection uniform mixing port.
4. The particle detection unit of claim 1, wherein the flow channel has at least two branches that are controlled by valves to communicate with and disconnect from the suction device.
5. The particle detection unit of any one of claims 1-4, wherein the substrate is a sheet material, and the substrate may enclose at least a portion of the flow channel.
6. The particle detection unit of claim 5, wherein the flow channel has a flow channel flux equivalent diameter in the range of 10-1000 μm.
7. The particle detection unit of any one of claims 1-4, wherein the suction device comprises a suction chamber disposed on the substrate and a sealing plug disposed on the suction chamber, the suction chamber being in communication with the flow channel; the sealing plug is used for connecting with a suction assembly for providing suction power.
8. The particle detection unit of any one of claims 1-4, further comprising a detection window disposed on the substrate, one end of the detection window being in communication with the flow channel, and the other end of the detection window being in communication with the suction device.
9. The particle detection unit of claim 8, wherein the other end of the detection window is in communication with the suction device through a connection channel.
10. The particle detection unit of claim 9, wherein a moisture sensitive detection member is disposed within the connection channel.
11. The particle detection unit of claim 10, wherein a filter is disposed within the suction device.
12. The particle detection unit of claim 8, further comprising a color substance disposed in the flow channel, or in the sample introduction device, or in the suction device, or in the blow-and-suck device, the color substance being configured to mix with a liquid analyte injected into the sample introduction device.
13. A mixing system, comprising:
a particle detection unit as claimed in any one of claims 1 to 12; and
the suction assembly comprises a suction power source, an aspirator and a suction needle device, wherein the suction needle device is used for being inserted into the suction device, the suction needle device is communicated with the aspirator, and the suction power source is used for driving the aspirator to act so as to suck air to the suction device through the suction needle device.
14. The mixing system of claim 13, wherein the aspiration power source is a first linear stepper motor, the aspirator includes a first cylinder and a first piston rod, one end of the first piston rod is disposed within the first cylinder, the other end of the first piston rod is disposed at an output end of the first linear stepper motor, and the aspiration needle device is in communication with the first cylinder.
15. The mixing system of claim 13, further comprising a blowing and sucking assembly, wherein the blowing and sucking assembly comprises a blowing and sucking power source, a blowing and sucking device and a blowing and sucking needle device, the blowing and sucking needle device is used for being inserted into the blowing and sucking device, the blowing and sucking needle device is communicated with the blowing and sucking device, and the blowing and sucking power source is used for driving the blowing and sucking device to act so as to blow or suck air to the blowing and sucking device through the blowing and sucking needle device.
16. The mixing system of claim 15, wherein the blowing and sucking power source is a second linear stepper motor, the blower comprises a second cylinder and a second piston rod, one end of the second piston rod is disposed in the second cylinder, and the other end of the second piston rod is disposed at an output end of the second linear stepper motor; the blow and suction needle device is communicated with the second cylinder body.
17. The mixing system of claim 16, further comprising a third power source and a drive plate, the suction and blow pin means being disposed on the drive plate, the third power source being configured to drive the drive plate to move in a direction toward or away from the base plate.
18. A mixing method, comprising the steps of:
injecting a liquid analyte into a sample injection device through a sample injection port of the sample injection device, wherein the sample injection device is arranged on a substrate, a flow channel is arranged in the substrate, and a suction device communicated with the sample injection device through the flow channel is also arranged on the substrate;
inserting a suction needle device into the suction apparatus;
the suction power source drives the suction device to act so as to suck the suction device through the suction needle device, and the liquid analyte in the sample feeding device enters the flow channel.
19. A mixing method, comprising the steps of:
injecting a liquid analyte into a sample injection device through a sample injection port of the sample injection device, wherein the sample injection device is arranged on a substrate, a flow channel is arranged in the substrate, and a suction device and a blowing-suction device which are respectively communicated with the sample injection device through the flow channel are also arranged on the substrate;
inserting a blowing and sucking needle-shaped device into a blowing and sucking device;
the blowing and sucking device is driven to act by the blowing and sucking power source so as to blow or exhaust air to the blowing and sucking device through the blowing and sucking needle-shaped device, so that the liquid analyte in the sample injection device flows to the inside of the blowing and sucking device through the flow channel or the liquid analyte in the blowing and sucking device flows to the inside of the sample injection device through the flow channel, and the liquid analytes are uniformly mixed;
inserting a suction needle device into the suction apparatus;
the suction power source drives the suction device to act so as to suck the suction device through the suction needle-shaped device, and the uniformly mixed liquid analyte in the sample introduction device enters the flow channel.
20. The mixing method of claim 19, comprising the step of
A color substance is disposed in the flow channel, the sample introduction device, the aspiration device, or the blow-and-suck device, the color substance being mixed with the liquid analyte.
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| CN202111236637.7A CN114018787B (en) | 2021-10-23 | 2021-10-23 | Particle detection unit, mixing system and mixing method |
| PCT/CN2022/126795 WO2023066388A1 (en) | 2021-10-23 | 2022-10-21 | Particle detection unit, detection plate and mixing method |
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| CN202111236637.7A CN114018787B (en) | 2021-10-23 | 2021-10-23 | Particle detection unit, mixing system and mixing method |
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| CN116355725A (en) * | 2023-03-07 | 2023-06-30 | 广州市艾贝泰生物科技有限公司 | Distributor, distributing device and distributing method |
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| CN116355725B (en) * | 2023-03-07 | 2024-04-05 | 广州市艾贝泰生物科技有限公司 | Distributor, distributing device and distributing method |
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