CN114009457B - Essential oil composition and application thereof - Google Patents
Essential oil composition and application thereof Download PDFInfo
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- CN114009457B CN114009457B CN202111244520.3A CN202111244520A CN114009457B CN 114009457 B CN114009457 B CN 114009457B CN 202111244520 A CN202111244520 A CN 202111244520A CN 114009457 B CN114009457 B CN 114009457B
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- China
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- essential oil
- oil composition
- mildew
- litsea cubeba
- oregano
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- 239000000341 volatile oil Substances 0.000 title claims abstract description 160
- 239000000203 mixture Substances 0.000 title claims abstract description 90
- 240000002262 Litsea cubeba Species 0.000 claims abstract description 29
- 235000012854 Litsea cubeba Nutrition 0.000 claims abstract description 29
- 235000013305 food Nutrition 0.000 claims abstract description 24
- 240000007673 Origanum vulgare Species 0.000 claims abstract description 22
- 235000013628 Lantana involucrata Nutrition 0.000 claims abstract description 21
- 235000006677 Monarda citriodora ssp. austromontana Nutrition 0.000 claims abstract description 21
- 241000228245 Aspergillus niger Species 0.000 claims abstract description 15
- 239000003921 oil Substances 0.000 claims abstract description 14
- 241000122824 Aspergillus ochraceus Species 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 239000000022 bacteriostatic agent Substances 0.000 claims description 5
- 108010010803 Gelatin Proteins 0.000 claims description 4
- ROOXNKNUYICQNP-UHFFFAOYSA-N ammonium persulfate Chemical compound [NH4+].[NH4+].[O-]S(=O)(=O)OOS([O-])(=O)=O ROOXNKNUYICQNP-UHFFFAOYSA-N 0.000 claims description 4
- 239000008273 gelatin Substances 0.000 claims description 4
- 229920000159 gelatin Polymers 0.000 claims description 4
- 235000019322 gelatine Nutrition 0.000 claims description 4
- 235000011852 gelatine desserts Nutrition 0.000 claims description 4
- 239000000017 hydrogel Substances 0.000 claims description 4
- 239000007921 spray Substances 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 239000003094 microcapsule Substances 0.000 claims description 3
- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 claims description 2
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 2
- 239000011837 N,N-methylenebisacrylamide Substances 0.000 claims description 2
- 235000010489 acacia gum Nutrition 0.000 claims description 2
- 239000001785 acacia senegal l. willd gum Substances 0.000 claims description 2
- 229910001870 ammonium persulfate Inorganic materials 0.000 claims description 2
- 238000009472 formulation Methods 0.000 claims description 2
- 239000003112 inhibitor Substances 0.000 claims description 2
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 claims description 2
- 239000002088 nanocapsule Substances 0.000 claims 1
- 230000003385 bacteriostatic effect Effects 0.000 abstract description 17
- 241000228197 Aspergillus flavus Species 0.000 abstract description 15
- 238000003860 storage Methods 0.000 abstract description 11
- 239000002636 mycotoxin Substances 0.000 abstract description 9
- 231100000678 Mycotoxin Toxicity 0.000 abstract description 8
- 230000002195 synergetic effect Effects 0.000 abstract description 8
- 230000002401 inhibitory effect Effects 0.000 abstract description 7
- 238000001228 spectrum Methods 0.000 abstract description 6
- 241000233866 Fungi Species 0.000 abstract description 5
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- 238000000034 method Methods 0.000 description 10
- 230000000694 effects Effects 0.000 description 9
- OQIQSTLJSLGHID-WNWIJWBNSA-N aflatoxin B1 Chemical compound C=1([C@@H]2C=CO[C@@H]2OC=1C=C(C1=2)OC)C=2OC(=O)C2=C1CCC2=O OQIQSTLJSLGHID-WNWIJWBNSA-N 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 241001529744 Origanum Species 0.000 description 7
- 235000011203 Origanum Nutrition 0.000 description 7
- 238000009825 accumulation Methods 0.000 description 7
- 239000002115 aflatoxin B1 Substances 0.000 description 7
- 229930020125 aflatoxin-B1 Natural products 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 7
- VYLQGYLYRQKMFU-UHFFFAOYSA-N Ochratoxin A Natural products CC1Cc2c(Cl)cc(CNC(Cc3ccccc3)C(=O)O)cc2C(=O)O1 VYLQGYLYRQKMFU-UHFFFAOYSA-N 0.000 description 6
- 238000001914 filtration Methods 0.000 description 6
- 239000012528 membrane Substances 0.000 description 6
- 238000002156 mixing Methods 0.000 description 6
- RWQKHEORZBHNRI-BMIGLBTASA-N ochratoxin A Chemical compound C([C@H](NC(=O)C1=CC(Cl)=C2C[C@H](OC(=O)C2=C1O)C)C(O)=O)C1=CC=CC=C1 RWQKHEORZBHNRI-BMIGLBTASA-N 0.000 description 6
- DAEYIVCTQUFNTM-UHFFFAOYSA-N ochratoxin B Natural products OC1=C2C(=O)OC(C)CC2=CC=C1C(=O)NC(C(O)=O)CC1=CC=CC=C1 DAEYIVCTQUFNTM-UHFFFAOYSA-N 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- 230000002265 prevention Effects 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- 230000001954 sterilising effect Effects 0.000 description 5
- 238000007789 sealing Methods 0.000 description 4
- 239000008223 sterile water Substances 0.000 description 4
- 241000607479 Yersinia pestis Species 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 235000013312 flour Nutrition 0.000 description 3
- 238000003958 fumigation Methods 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000010998 test method Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 208000003643 Callosities Diseases 0.000 description 2
- 241000223195 Fusarium graminearum Species 0.000 description 2
- 241000233732 Fusarium verticillioides Species 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 2
- 241000228143 Penicillium Species 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 125000003118 aryl group Chemical group 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- NEHNMFOYXAPHSD-UHFFFAOYSA-N citronellal Chemical compound O=CCC(C)CCC=C(C)C NEHNMFOYXAPHSD-UHFFFAOYSA-N 0.000 description 2
- QMVPMAAFGQKVCJ-UHFFFAOYSA-N citronellol Chemical compound OCCC(C)CCC=C(C)C QMVPMAAFGQKVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000008367 deionised water Substances 0.000 description 2
- 229910021641 deionized water Inorganic materials 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- XMGQYMWWDOXHJM-UHFFFAOYSA-N limonene Chemical compound CC(=C)C1CCC(C)=CC1 XMGQYMWWDOXHJM-UHFFFAOYSA-N 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- MGSRCZKZVOBKFT-UHFFFAOYSA-N thymol Chemical compound CC(C)C1=CC=C(C)C=C1O MGSRCZKZVOBKFT-UHFFFAOYSA-N 0.000 description 2
- QMVPMAAFGQKVCJ-SNVBAGLBSA-N (R)-(+)-citronellol Natural products OCC[C@H](C)CCC=C(C)C QMVPMAAFGQKVCJ-SNVBAGLBSA-N 0.000 description 1
- XWIYFDMXXLINPU-UHFFFAOYSA-N Aflatoxin G Chemical compound O=C1OCCC2=C1C(=O)OC1=C2C(OC)=CC2=C1C1C=COC1O2 XWIYFDMXXLINPU-UHFFFAOYSA-N 0.000 description 1
- 102000040350 B family Human genes 0.000 description 1
- 108091072128 B family Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 208000005623 Carcinogenesis Diseases 0.000 description 1
- WTEVQBCEXWBHNA-UHFFFAOYSA-N Citral Natural products CC(C)=CCCC(C)=CC=O WTEVQBCEXWBHNA-UHFFFAOYSA-N 0.000 description 1
- 235000005979 Citrus limon Nutrition 0.000 description 1
- 244000131522 Citrus pyriformis Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- XYFCBTPGUUZFHI-UHFFFAOYSA-N Phosphine Chemical compound P XYFCBTPGUUZFHI-UHFFFAOYSA-N 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 208000031320 Teratogenesis Diseases 0.000 description 1
- 239000005844 Thymol Substances 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- JGQFVRIQXUFPAH-UHFFFAOYSA-N beta-citronellol Natural products OCCC(C)CCCC(C)=C JGQFVRIQXUFPAH-UHFFFAOYSA-N 0.000 description 1
- 229940116229 borneol Drugs 0.000 description 1
- 230000036952 cancer formation Effects 0.000 description 1
- 231100000504 carcinogenesis Toxicity 0.000 description 1
- 229940117948 caryophyllene Drugs 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 229940043350 citral Drugs 0.000 description 1
- 235000000983 citronellal Nutrition 0.000 description 1
- 229930003633 citronellal Natural products 0.000 description 1
- 235000000484 citronellol Nutrition 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 238000004320 controlled atmosphere Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000002845 discoloration Methods 0.000 description 1
- OPGYRRGJRBEUFK-UHFFFAOYSA-L disodium;diacetate Chemical compound [Na+].[Na+].CC([O-])=O.CC([O-])=O OPGYRRGJRBEUFK-UHFFFAOYSA-L 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- WTEVQBCEXWBHNA-JXMROGBWSA-N geranial Chemical compound CC(C)=CCC\C(C)=C\C=O WTEVQBCEXWBHNA-JXMROGBWSA-N 0.000 description 1
- 235000011868 grain product Nutrition 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 235000001510 limonene Nutrition 0.000 description 1
- 229940087305 limonene Drugs 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000005399 mechanical ventilation Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- JPTOCTSNXXKSSN-UHFFFAOYSA-N methylheptenone Chemical compound CCCC=CC(=O)CC JPTOCTSNXXKSSN-UHFFFAOYSA-N 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 238000012536 packaging technology Methods 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 238000001121 post-column derivatisation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 235000017454 sodium diacetate Nutrition 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
- 235000007586 terpenes Nutrition 0.000 description 1
- 229960000790 thymol Drugs 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 238000009461 vacuum packaging Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/24—Lauraceae [Laurel family], e.g. laurel, avocado, sassafras, cinnamon or camphor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B9/00—Preservation of edible seeds, e.g. cereals
- A23B9/16—Preserving with chemicals
- A23B9/24—Preserving with chemicals in the form of liquids or solids
- A23B9/26—Organic compounds; Microorganisms; Enzymes
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Chemical & Material Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Biotechnology (AREA)
- Environmental Sciences (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Organic Chemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses an essential oil composition and application thereof, wherein the essential oil composition comprises litsea cubeba essential oil and oregano essential oil, and the essential oil composition contains 10-30% of litsea cubeba essential oil and 70-90% of oregano essential oil by mass. The essential oil composition can fully exert the synergistic effect of the two essential oils, not only has the characteristics of green and safety of the plant essential oil, but also can improve the mildew-proof and bacteriostatic properties and broaden the bacteriostatic spectrum while reducing the using amount of the essential oil, particularly shows high-efficiency mildew-proof and bacteriostatic properties in the aspects of inhibiting ochratoxin, aspergillus flavus and aspergillus niger, greatly reduces the using concentration and remarkably reduces the using cost compared with the aspect of independently using any one of the essential oils, so that the essential oil composition can control and improve the harm and pollution of fungi and mycotoxin in the storage, transportation and circulation links of grain and oil foods in China, can ensure the safety of the grain and oil foods, and promotes the sustainable development of society.
Description
Technical Field
The invention relates to the field of mildew prevention and bacteriostasis of grain and oil food. More particularly, it relates to an essential oil composition and its application.
Background
According to the measurement and calculation of the large countries for grain production, storage and consumption, the loss of grains in China caused by pests and mildewing accounts for 1.5-3% of the total yield every year, and the pollution of the pests, fungi and mycotoxins thereof is a main factor threatening the safety of grain storage, transportation and circulation links and causing grain loss. According to FAO estimates, at least 25% of the world's food is contaminated with mycotoxins and at least 2% of the food is spoilt every year due to mildew. Many fungi can cause discoloration and fever of grains, and simultaneously generate a large amount of mycotoxin, thereby bringing about food safety problems and environmental pollution due to improper control measures. The mycotoxin has strong toxicity, high chemical stability, long half-life period in animals and human bodies, and has the effects of carcinogenesis, teratogenesis or mutagenesis and the like, and various complications are caused. Storage of food and grain products by controlling environmental conditions such as temperature, humidity, air, etc. is the most preferred method. The modernized grain storage and transportation technology guarantee system is widely applied to large-scale grain bins, and comprises the following components: detecting grain condition, storing grain mechanical ventilation technology, hydrogen phosphide circulation fumigation technology, grain cooler low-temperature grain storage technology and green nitrogen-filled controlled atmosphere storage technology. The technical system can reduce the temperature of the grain, simultaneously maintain and properly adjust the moisture of the grain, delay the metabolism of the grain, maintain and improve the quality of the stored grain, and improve the safety and stability of the grain storage. The product has wide application in finished product of grain and oil, various packaging technologies (such as vacuum packaging technology) and the like, and grain mildew inhibitors (such as sodium diacetate and the like), prolongs the shelf life, and keeps better quality of the product. These techniques are not only green and pollution-free, but also very effective. However, the respiration of the organisms inside or on the surface of the food and grain and oil and the heat and dew condensation caused by the temperature and humidity change of the environment during the storage process can cause serious pollution of the food by the pests, fungi and mycotoxins, and the application of the technical method can not be completely solved. Therefore, the safe and efficient prevention and control technology for the fungal and mycotoxin hazards in the grain and oil food is developed, and has great significance for guaranteeing the safety of the grain and oil food in China.
Essential oil is a volatile aromatic substance extracted from flowers, leaves, stems, roots or fruits of aromatic plants, and mainly contains terpenes, phenols, aldehydes, alcohols, esters and other organic substances. At present, the essential oil has a lot of related researches and application reports on mildew prevention and bacteriostasis, and the researches show that the single essential oil has an unsatisfactory mildew prevention and bacteriostasis effect and an insufficient wide bacteriostasis spectrum, and the bacteriostasis effect can be improved when different essential oils are prepared into compound essential oil.
Therefore, it is very important to find an efficient and broad-spectrum essential oil composition for the mildew-proof and bacteriostatic field of grain and oil food.
Disclosure of Invention
The essential oil composition can fully exert the synergistic effect of two essential oils, has the characteristics of greenness and safety of plant essential oil, can improve the mildew-proof and bacteriostatic performance while reducing the consumption of the essential oil, widens the bacteriostatic spectrum, and particularly shows high-efficiency mildew-proof and bacteriostatic performance in the aspects of inhibiting aspergillus ochraceus, aspergillus flavus and aspergillus niger.
The second purpose of the invention is to provide a mildew-proof bacteriostatic agent containing the essential oil composition.
The third purpose of the invention is to provide the application of the essential oil composition in preventing and controlling the mildew of the grain and oil food.
In order to achieve the first purpose, the invention adopts the following technical scheme:
the invention provides an essential oil composition which is characterized by comprising litsea cubeba essential oil and oregano essential oil.
The essential oil composition of the litsea cubeba essential oil and the oregano essential oil provided by the invention has high-efficiency mildew-proof and bacteriostatic performance, is green and safe, has a wide bacteriostatic spectrum, particularly shows high-efficiency mildew-proof and bacteriostatic performance in the aspect of inhibiting aspergillus ochraceus, aspergillus flavus and aspergillus niger, greatly reduces the use concentration and remarkably reduces the use cost compared with the mode of singly using any one essential oil.
Further, the essential oil composition contains 10-30% of litsea cubeba essential oil and 70-90% of oregano essential oil by mass fraction.
Further, the essential oil composition contains 15-25% of litsea cubeba essential oil and 75-85% of oregano essential oil by mass.
Further, the essential oil composition contains 17-23% of litsea cubeba essential oil and 77-83% of oregano essential oil by mass fraction.
In order to achieve the second object, the present invention provides a mildew-proof bacteriostatic agent comprising the above essential oil composition.
Furthermore, the mildew-proof bacteriostatic agent is prepared into dosage forms including but not limited to nano microcapsules, hydrogel agents or spray.
Further, each component in the nanometer microcapsule is 2-5% of the essential oil composition by mass percent; 0.5 to 3 percent of gelatin; 0.5 to 3 percent of Arabic gum; tween-801% -5%, and the balance of water.
Further, each component in the hydrogel agent is 2-5% of the essential oil composition by mass percent; 2 to 5 percent of gelatin; 2 to 5 percent of acrylic acid; tween-800.2% -1%; 0.4 to 1.0 percent of N, N-methylene bisacrylamide; 0.5 to 1 percent of ammonium persulfate; the remainder being water.
Further, each component in the spray comprises 2-5% of an essential oil composition by mass percent; 2-5% of 75% alcohol; tween-800.2% -1%; the remainder being water.
In order to achieve the third purpose, the invention provides the application of the essential oil composition in preventing and treating the food mould.
Further, the food and grain mold includes, but is not limited to, aspergillus ochraceus, aspergillus flavus, aspergillus niger, fusarium graminearum, penicillium or fusarium moniliforme.
The essential oil composition provided by the invention can effectively inhibit growth and propagation of aspergillus ochraceus, aspergillus flavus and aspergillus niger and accumulation of toxins, can be widely applied to the field of mildew prevention and inhibition of grain and oil food, and has a good effect on inhibiting fusarium graminearum, penicillium, fusarium moniliforme and other molds, a wide inhibition spectrum and a wide application prospect.
The invention has the following beneficial effects:
the invention discloses an essential oil composition and application thereof, wherein the essential oil composition comprises litsea cubeba essential oil and oregano essential oil, and the essential oil composition contains 10-30% of litsea cubeba essential oil and 70-90% of oregano essential oil by mass fraction. The essential oil composition can fully exert the synergistic effect of two essential oils, not only has the characteristics of green and safety of plant essential oil, but also can improve the mildew-proof and bacteriostatic performance and broaden the bacteriostatic spectrum while reducing the using amount of the essential oil, particularly shows high-efficiency mildew-proof and bacteriostatic performance in the aspects of inhibiting ochratoxin, aspergillus flavus and aspergillus niger, greatly reduces the using concentration and remarkably reduces the using cost compared with the way of independently using any essential oil, so that the essential oil composition not only can control and improve the harm and pollution of fungi and mycotoxin in the storage, transportation and circulation links of grain and oil foods in China, but also can ensure the safety of the grain and oil foods, and promotes the sustainable development of society.
Detailed Description
In order to more clearly illustrate the invention, the invention is further described below in connection with preferred embodiments. It is to be understood by persons skilled in the art that the following detailed description is illustrative and not restrictive, and is not to be taken as limiting the scope of the invention.
The instruments and reagents adopted by the invention are all common commercial products and can be purchased in the market. The litsea cubeba essential oil is yellowish and has strong lemon flavor, and comprises the following main components: citral (70% -80%), limonene (9.25%), methylheptenone (2.50%), citronellol (2.44%), citronellal (1.0%), and the like, available from Shanghai-derived leaf Biotech, Inc.; the oregano essential oil is yellowish red or brownish red, and mainly comprises the following components: carvacrol-like (80.40%), thymol (6.60%), p-aragonine (5.21%), a-caryophyllene (2.38%), 1-borneol (1.21%), etc., available from Shanghai Michelin Biochemical technology, Inc.
Example 1
Accurately sucking 10 wt% of litsea cubeba essential oil and 90 wt% of origanum essential oil according to mass fraction, filtering with a 0.22 mu m microporous filter membrane, and uniformly mixing in a vortex mixer to obtain the essential oil composition.
Example 2
Accurately sucking 20 wt% of litsea cubeba essential oil and 80 wt% of origanum vulgaris essential oil according to mass fraction, filtering with a 0.22 mu m microporous filter membrane, and uniformly mixing in a vortex mixer to obtain the essential oil composition.
Comparative example 1
Accurately absorbing 5 wt% of litsea cubeba essential oil and 95 wt% of origanum essential oil according to mass fraction, filtering the mixture through a 0.22 mu m microporous filter membrane, and uniformly mixing the mixture in a vortex mixer to obtain the essential oil composition.
Comparative example 2
Accurately absorbing 40 wt% of litsea cubeba essential oil and 60 wt% of origanum essential oil according to mass fraction, filtering the mixture through a 0.22 mu m microporous filter membrane, and uniformly mixing the mixture in a vortex mixer to obtain the essential oil composition.
Comparative example 3
Accurately absorbing 60 wt% of litsea cubeba essential oil and 40 wt% of origanum vulgaris essential oil according to mass fraction, filtering the mixture through a 0.22 mu m microporous filter membrane, and uniformly mixing the mixture in a vortex mixer to obtain the essential oil composition.
Comparative example 4
Accurately absorbing 90 wt% of litsea cubeba essential oil and 10 wt% of origanum essential oil according to mass fraction, filtering the mixture through a 0.22 mu m microporous filter membrane, and uniformly mixing the mixture in a vortex mixer to obtain the essential oil composition.
Test example 1
This test example was used to study the synergistic effect of the essential oil composition. The test method comprises the following steps: a gas phase fumigation method is adopted for carrying out mildew-proof and bacteriostasis experiments, a PDA culture medium which is cooled to 60 ℃ after sterilization is poured into aseptic culture dishes, 30mL of each culture dish is prepared into a solid culture medium, then litsea cubeba essential oil, oregano essential oil and the essential oil composition of example 2 (namely the essential oil composition of 20 wt% of litsea cubeba essential oil and 80 wt% of oregano essential oil) are respectively added onto aseptic filter paper sheets on the covers of the culture dishes, inverted culture is carried out for 8 days, if no macroscopic colony grows, the minimum bacteriostasis concentration of the essential oil composition is obtained, and the bacteriostasis effects of the three essential oils are evaluated through a fractional bacteriostasis concentration (FIC) index. When FIC is less than or equal to 0.5, the synergistic effect is achieved; when FIC is more than 0.5 and less than or equal to 1, the additive effect is obtained; when FIC is more than 1 and less than or equal to 2, the effect is irrelevant; when FIC is more than 2, antagonism is achieved; the calculation formula of FIC is
TABLE 1 comparison of synergistic experiments for Litsea cubeba essential oil, Oregano essential oil and essential oil composition
As shown in Table 1, the essential oil composition prepared in example 2 can effectively inhibit the growth of Aspergillus ochraceus, Aspergillus flavus and Aspergillus niger, the minimum inhibitory concentrations of the essential oil composition are 0.107mg/mL, 0.125mg/mL and 0.143mg/mL respectively, compared with the application of Litsea cubeba essential oil alone, the minimum inhibitory concentrations of Aspergillus ochraceus, Aspergillus flavus and Aspergillus niger are 1.072mg/mL, 1.251mg/mL and 1.608mg/mL respectively, and the application of Oregano essential oil alone is 0.968mg/mL, 0.581mg/mL and 0.465mg/mL respectively, the reduction of the application concentration of the essential oil is 69% -91%, which shows that after the Litsea cubeba essential oil and the Oregano origanum essential oil are compounded, a remarkable synergistic effect is generated, the MIC of the two is remarkably reduced, the FIC of the two is not expected, and according to the evaluation of FIC index, the essential oil composition has a synergistic effect on bacteriostasis of aspergillus ochraceus and aspergillus niger and an additive effect on bacteriostasis of aspergillus flavus.
Test example 2
The essential oil compositions prepared in the embodiments 1-2 and the comparative examples 1-4 are used for performing mildew-proof and bacteriostatic experiments, and the test bacteria are aspergillus ochraceus, aspergillus flavus and aspergillus niger and are provided by the institute of grain and oil processing of the national institute of science and research of the food and material storage.
The test method comprises the following steps: performing mould-proof and bacteriostatic experiments by adopting a gas-phase fumigation method, pouring the sterilized PDA culture medium cooled to 60 ℃ into sterile culture dishes, and preparing a solid culture medium by 30mL of each culture dish. Respectively sucking 100 μ L of Aspergillus ochraceus, Aspergillus flavus and Aspergillus niger spore liquid (concentration is 10% 6 -10 7 cfu/mL) in a solid culture medium, evenly coating the solid culture medium, inverting the solid culture medium, placing a sterile filter paper sheet with the diameter of 1cm in the center of a culture dish cover, respectively sucking 20 mu L of the prepared essential oil composition on the filter paper sheet, sealing the filter paper sheet by using a sealing film, culturing the filter paper sheet at the constant temperature of 30 ℃ for 8 days, and measuring the diameter of a bacteriostatic circle by using a cross method, wherein the obtained result is shown in Table 2. As shown in Table 2, the essential oil composition has stronger bacteriostatic performance on aspergillus ochraceus, aspergillus flavus and aspergillus niger within the proportion range of the essential oil composition protected by the invention, and is obviously superior to comparisonThe data of examples 1 to 4 show that although the composite essential oil of the litsea cubeba essential oil and the oregano essential oil is also adopted in comparative examples 1 to 4, the proportion of the composite essential oil is not within the protection range of the invention, and no significant antibacterial performance is generated, while the proportion of the essential oil compositions prepared in examples 1 and 2 is within the protection range of the invention, the antibacterial performance is obviously superior to that of the essential oil compositions in comparative examples 1 to 4, because the proportion range provided by the invention is obtained through a large number of experiments, and has unexpected technical effects.
TABLE 2 comparison of zone of inhibition diameters for Aspergillus ochraceus, Aspergillus flavus and Aspergillus niger
Test example 3
This test example was conducted to examine the effect of the essential oil composition on accumulation of ochratoxin a in maize. The test method comprises the following steps: inoculating Aspergillus ochraceus spore to PDA culture medium, culturing at 30 deg.C for 3-5 days, collecting spore with inoculating loop to 1mL sterile water, counting with optical microscope, and adjusting spore concentration to 5 × 10 5 cfu/mL. Grinding corn into powder, taking 250mL conical bottles, adding 20g of corn flour into each conical bottle, preparing deionized water, and sterilizing at 121 ℃ for 30 min. After sterilization, the flask was allowed to stand at room temperature, and 10mL of sterile water was added to each flask in a clean bench to adjust the moisture content of the corn to 50%. After stirring well with a glass rod, 1mL of spore suspension was added. The essential oil composition prepared in example 2 was added to a piece of filter paper suspended in a triangular flask at various concentrations, sealed with a sealing film, and left to stand for 14 days at 30 ℃. Corn flour without essential oil composition as control, inactivating culture medium after culture, and measuring ochratoxin A in GB 5009.96-2016 (first method immunoaffinity chromatography liquid chromatography for purification)) The content of ochratoxin A is detected, and the obtained result is shown in table 3. Wherein the MIC of the essential oil composition to Aspergillus ochraceus is 0.107mg/mL, the corn-1, the corn-2, the corn-3 and the corn-4 are corns containing the essential oil compositions at different concentrations, and the corn-CK is corn without the essential oil composition.
TABLE 3 comparison of essential oil composition versus ochratoxin A accumulation at different concentrations
| Concentration of essential oil composition | Ochratoxin A (mu g/kg) | |
| Corn matrix | - | 0 |
| corn-CK | 0 | 9.48 |
| Corn-1 | 1/8MIC | 6.12 |
| Corn-2 | 1/4MIC | 0.34 |
| Corn-3 | 1/2MIC | 0 |
| Corn-4 | 1MIC | 0 |
As is apparent from Table 3, the essential oil composition prepared in example 2 was able to inhibit the accumulation of ochratoxin A, and the content of ochratoxin A was close to the detection limit of 0.30ug/kg when the concentration of the compounded essential oil reached 1/4 MIC.
Test example 4
This test example was conducted to examine the effect of the essential oil composition on the accumulation of aflatoxin B1 in corn. Inoculating Aspergillus flavus spore to PDA culture medium, culturing at 30 deg.C for 3-5 days, collecting spore with inoculating loop to 1mL sterile water, counting with optical microscope, and adjusting spore concentration to 5 × 10 5 cfu/mL. Grinding corn into powder, taking 250mL conical bottles, adding 20g of corn flour into each conical bottle, preparing deionized water, and sterilizing at 121 ℃ for 30 min. After sterilization, the flask was allowed to stand at room temperature, and 10mL of sterile water was added to each flask in a clean bench to adjust the moisture content of the corn to 50%. After stirring well with a glass rod, 1mL of spore suspension was added. The essential oil composition prepared in example 2 was added to a piece of filter paper suspended in a triangular flask at various concentrations, sealed with a sealing film, and left to stand for 14 days at 30 ℃. Corn meal without treatment with the essential oil composition was used as a control. After the culture is finished, the culture medium is inactivated, the content of aflatoxin B1 is detected according to the determination of aflatoxin B family and aflatoxin G family in GB 5009.22-2016 food (a third method high performance liquid chromatography-post column derivatization method), and the obtained result is shown in Table 4. Wherein the MIC of the essential oil composition to Aspergillus flavus is 0.125mg/mL, the corn-1, the corn-2, the corn-3 and the corn-4 are corns containing the essential oil compositions at different concentrations, and the corn-CK is corn without the essential oil composition.
TABLE 4 comparison of the accumulation of aflatoxin B1 by essential oil compositions at different concentrations
| Concentration of essential oil composition | Aflatoxin B1 (ug/kg) | |
| corn-CK | 0 | 83.48 |
| Corn-1 | 1/4MIC | 81.10 |
| Corn-2 | 1/2MIC | 10.72 |
| Corn-3 | 1MIC | 1.30 |
| Corn-4 | 2MIC | 2.05 |
As shown in Table 4, the essential oil composition can inhibit the accumulation of aflatoxin B1, when the concentration of the essential oil composition reaches 1/4MIC, the content of aflatoxin B1 is basically the same as that of a control group, and when the concentration is more than or equal to 1/2MIC, the content of aflatoxin B1 is obviously reduced.
It should be understood that the above-mentioned embodiments of the present invention are only examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention, and it will be obvious to those skilled in the art that other variations or modifications may be made on the basis of the above description, and all embodiments may not be exhaustive, and all obvious variations or modifications may be included within the scope of the present invention.
Claims (10)
1. The application of the essential oil composition in preventing and treating food mildew is characterized in that the essential oil composition comprises, by mass, 10-30% of litsea cubeba essential oil and 70-90% of oregano essential oil;
the food mould is Aspergillus ochraceus and Aspergillus niger.
2. The use according to claim 1, wherein the essential oil composition comprises 15-25% of litsea cubeba essential oil and 75-85% of oregano essential oil by mass fraction.
3. The use according to claim 1, characterized in that the essential oil composition is, in mass fraction, 17 to 23% of litsea cubeba essential oil and 77 to 83% of oregano essential oil.
4. The application of the mildew-proof bacteriostatic agent in preventing and treating the mildew of grain and oil food is characterized in that the mildew-proof bacteriostatic agent contains an essential oil composition; the essential oil composition comprises, by mass, 10-30% of litsea cubeba essential oil and 70-90% of oregano essential oil;
the food mould is Aspergillus ochraceus and Aspergillus niger.
5. The use according to claim 4, wherein the essential oil composition comprises 15-25% of litsea cubeba essential oil and 75-85% of oregano essential oil by mass fraction.
6. The use according to claim 4, wherein the essential oil composition comprises, in parts by mass, 17 to 23% of litsea cubeba essential oil and 77 to 83% of oregano essential oil.
7. The use of claim 4, wherein the mildew inhibitor is formulated as a nano microcapsule, a hydrogel formulation or a spray.
8. The application of claim 7, wherein the nanocapsule comprises 2-5% of an essential oil composition by mass; 0.5% -3% of gelatin; 0.5% -3% of Arabic gum; tween-801% -5%, and the balance of water.
9. The application of claim 7, wherein the hydrogel agent comprises 2-5% by mass of an essential oil composition; 2% -5% of gelatin; 2% -5% of acrylic acid; tween-800.2% -1%; 0.4 to 1.0 percent of N, N-methylene bisacrylamide; 0.5 to 1 percent of ammonium persulfate; the remainder being water.
10. The use according to claim 7, wherein the components in the spray comprise, by mass, 2% to 5% of an essential oil composition; 2-5% of 75% alcohol; tween-800.2% -1%; the remainder being water.
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