CN114002382A - Method for detecting high-temperature stability of rice photosynthetic capacity - Google Patents
Method for detecting high-temperature stability of rice photosynthetic capacity Download PDFInfo
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- CN114002382A CN114002382A CN202111232821.4A CN202111232821A CN114002382A CN 114002382 A CN114002382 A CN 114002382A CN 202111232821 A CN202111232821 A CN 202111232821A CN 114002382 A CN114002382 A CN 114002382A
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- 230000000243 photosynthetic effect Effects 0.000 title claims abstract description 67
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 38
- 235000009566 rice Nutrition 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 25
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 66
- 238000005259 measurement Methods 0.000 claims abstract description 42
- 239000000463 material Substances 0.000 claims abstract description 13
- 238000012545 processing Methods 0.000 claims abstract description 4
- 230000007246 mechanism Effects 0.000 claims description 52
- 238000009434 installation Methods 0.000 claims description 9
- 238000005286 illumination Methods 0.000 claims description 7
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 6
- 241000196324 Embryophyta Species 0.000 claims description 4
- 238000005096 rolling process Methods 0.000 claims description 4
- 230000003044 adaptive effect Effects 0.000 claims description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 3
- 239000001569 carbon dioxide Substances 0.000 claims description 3
- 238000001514 detection method Methods 0.000 claims description 2
- 125000004122 cyclic group Chemical group 0.000 abstract description 4
- 238000002474 experimental method Methods 0.000 abstract description 4
- 238000012258 culturing Methods 0.000 abstract 1
- 238000001179 sorption measurement Methods 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 210000001503 joint Anatomy 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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Abstract
The invention discloses a method for detecting high-temperature stability of rice photosynthetic capacity, which relates to the technical field of rice experiments and comprises the steps of presetting and arranging experiment conditions, adaptively culturing rice, preparing materials to be detected for leaves, controlling a portable photosynthetic determinator by a cyclic determination device to continuously determine photosynthetic rate at high temperature, processing and comparing data and the like. The portable photosynthetic apparatus is controlled by the cyclic measuring device to perform intermittent circumferential rotation, the rice plants surrounding the portable photosynthetic apparatus are measured one by one in the rotating process, the photosynthetic rate parameters of the surrounding rice plants can be rapidly and continuously measured, the time consumption of single-batch measurement is reduced, and the measurement time difference among a plurality of batches is small; the invention does not need manual measurement for multiple times and multiple samples one by one at high temperature, can automatically complete circular continuous measurement, saves time and labor, reduces errors of manual measurement, and improves the accuracy and stability of photosynthetic parameter measurement.
Description
Technical Field
The invention relates to the technical field of rice experiments, in particular to a method for detecting high-temperature stability of rice photosynthetic capacity.
Background
The conventional rice plant photosynthetic parameter is mostly determined by adopting a portable photosynthetic rate determinator, the sword leaves of rice are manually clamped and determined one by utilizing a leaf chamber at the front end of a handheld part of the portable photosynthetic rate determinator, and the sword leaves are determined on living plants and cannot be damaged because the sword leaves are only one and need to be determined for many times.
In order to research the influence of high temperature on the photosynthetic capacity of rice, a high-temperature climate needs to be built in an artificial climate chamber, then the rice is moved into an artificial high-temperature environment, the photosynthesis parameters of the sword leaves of the rice are measured for multiple times in a single day, and the photosynthesis stability of the rice in the high-temperature climate is observed by continuously measuring for multiple days. The measurement is usually carried out once every 1.5-2 h under the illumination condition, 4-6 times every day and 3-5 days continuously. The photosynthetic capacity high-temperature stability of each rice material can be obtained by comparing the photosynthetic parameters continuously collected for multiple times in multiple days of each rice material back and forth, and multiple groups of data measured by multiple rice materials in parallel experiments can be compared for screening the rice materials with better photosynthetic capacity high-temperature stability.
However, the determination of photosynthetic parameters of rice plants is still performed manually piece by piece, when multiple materials and multiple samples are simultaneously measured, because the number of samples and the number of times of measurement are large, not only is manual measurement time-consuming and labor-consuming, but more importantly, the time span for measuring all samples at a time is increased, the time point difference corresponding to the parameters measured before and after is large, the measured values of the parameters are likely to be inaccurate, the parameters measured in the same batch cannot be subjected to parallel comparison, the time difference measured before and after different samples of the same material cannot be staggered, and the requirement of measuring once every two hours or shorter time cannot be met.
Disclosure of Invention
The invention aims to provide a method for detecting the high-temperature stability of the photosynthetic capacity of rice, and solves the problems that the manual measurement of the photosynthetic parameters of the rice in a single batch consumes too long time, and the measurement interval time cannot be accurately controlled.
A method for detecting the high-temperature stability of the photosynthetic capacity of rice comprises the following steps:
step one, installing a portable photosynthetic determinator on a cycle determination device, and placing the cycle determination device in an artificial climate chamber for later use; the portable photosynthetic rate tester adopts an external carbon dioxide system;
step two, transplanting rice plants into a pot for planting, after carrying out adaptive culture in an artificial climate chamber for 7 days, placing a plurality of rice plants to be detected outside a circular surrounding manner in a circulation determination device, sticking magnetic sheets at the upper end and the lower end of the back of the sword leaves of the single rice plant, adsorbing the sword leaves on the upper part of a leaf pressing mechanism through the magnetic sheets, and completely drawing out and unfolding the sword leaves of the rice plants to be detected; the illumination intensity of the artificial climate chamber is 16000Lx, the temperature is 25 ℃, the humidity is 60-70%, and the illumination is 14 h/d;
step three, continuously measuring the photosynthetic rate at high temperature: only adjusting the temperature parameter of the artificial climate chamber to 39 ℃, measuring the photosynthetic rate and stomatal conductance of the flag leaves of different rice materials every 2 hours from 9 o 'clock to 17 o' clock, repeatedly measuring at least 10 plants of each rice material, and selecting the same part of each rice flag leaf to carry out multiple measurements during measurement, wherein the measured part is the middle part of the flag leaf; the portable photosynthetic apparatus is controlled by the circulation measuring device to make intermittent circular rotation, the rice plants surrounding the portable photosynthetic apparatus are measured one by one in the rotation process, the rotation is stopped when the portable photosynthetic apparatus is close to the rice plant to be measured, the circulation measuring device presses down a single sword leaf to be horizontally arranged in front of a leaf chamber of the portable photosynthetic apparatus, the circulation measuring device controls the leaf chamber to extend forwards and press and measure the photosynthetic parameters of the sword leaf, after the measurement, the leaf chamber is retracted and separated from the leaf for resetting, the sword leaf is lifted by the circulation measuring device to give way for the portable photosynthetic apparatus, the portable photosynthetic apparatus continuously rotates until the sword leaf is close to the next rice plant to be measured, and the detection steps are repeated to continuously measure the photosynthetic rate parameters of the surrounding rice plant one by one;
and step four, the measured photosynthetic rate parameters are led into a computer for processing and analysis, and then the photosynthetic capacity of the rice plant under the high-temperature condition can be obtained.
Preferably, the circulation measuring device comprises a bottom plate, a stand column is arranged in the center of the bottom plate, a plurality of spokes are uniformly arranged on the upper portion of the stand column along the radial direction, arc-shaped blocks are arranged at the far ends of the spokes, annular slide rails are arranged below the arc-shaped blocks, slide grooves are formed in the annular slide rails, the arc-shaped blocks are connected in the slide grooves in a sliding manner, trapezoidal protrusions are arranged at the bottom of the annular slide rails, inner gear rings are arranged on the inner sides of the annular slide rails, a first motor is arranged on the bottom plate, a first gear is arranged on an output shaft of the first motor and meshed with the inner gear rings, leaf pressing mechanisms are arranged on the arc-shaped blocks and sleeved outside the annular slide rails, a third motor is arranged at the top of the stand column, a top plate is coaxially and rotatably connected with the top end of the stand column, the bottom surface of the top plate is connected with an output shaft of the third motor, and connecting rods are arranged on the outer side of the top plate along the radial direction of the top plate, the remote center of the connecting rod is provided with a rotating block, the rotating block is provided with a telescopic mechanism and a pressing frame, the telescopic mechanism is provided with a measuring mechanism, the measuring mechanism is controlled by the pressing frame to be pressed or reset and opened in the telescopic process, the trapezoidal protrusion transversely arranges the adsorbed blade in front of the advancing path of the measuring mechanism when passing through the blade pressing mechanism, and the blade pressing mechanism resets and lifts when the trapezoidal protrusion is separated from the blade pressing mechanism.
Preferably, the leaf pressing mechanism comprises an installation block arranged on the arc block, the installation block is connected with two lifting rods in a sliding manner, the two lifting rods are symmetrically arranged inside and outside the annular slide rail, a cross roller is arranged between the lower ends of the two lifting rods, the cross roller is arranged below the annular slide rail, springs I are arranged between the two ends of the cross roller and the installation block, the springs are sleeved outside the lifting rods, when the trapezoidal convex block passes through the position of the cross roller, the cross roller is in rolling butt joint with the bottom surface of the trapezoidal convex block, a rack is arranged at the upper end of the lifting rod outside the annular slide rail, a vertical plate is arranged on one side of the installation block, the vertical plate is arranged outside the annular slide rail, the top of the vertical plate is rotatably connected with a rotating shaft, a first gear is arranged at one end of the rotating shaft and is meshed with the rack, a concave frame is arranged in the middle of the rotating shaft along the radial direction, and an adsorption groove is arranged on the concave frame, when the concave frame is transversely arranged in front of the measuring mechanism, the measuring mechanism extends forwards to enter the gap of the concave frame to clamp the blade for measurement.
Preferably, measuring mechanism includes the leaf room, grab handle and pressure handle, be equipped with spring two between pressure handle and the grab handle, the grab handle is located telescopic machanism, the front end of grab handle is located to the leaf room, the front end of pressing the handle is connected with the upper portion of leaf room, the rear end of pressing the handle is equipped with the depression bar, the both ends and the rolling butt of pressure frame of depression bar, the front end of pressing the frame is the wedge, the leaf room passes through the hose and the electric wire is connected with the host computer, the host computer is located on the roof.
Preferably, telescopic machanism is including locating the slider under the grab handle, the bottom and the turning block sliding connection of slider, the turning block internal rotation is connected with the screw rod, screw rod and slider threaded connection, the rear end of turning block is equipped with motor two, the output shaft and the screw rod tip of motor two are connected.
The invention has the advantages that:
the portable photosynthetic apparatus is controlled by the cyclic measuring device to perform intermittent circumferential rotation, the rice plants surrounding the portable photosynthetic apparatus are measured one by one in the rotating process, the photosynthetic rate parameters of the surrounding rice plants can be rapidly and continuously measured, the time consumption of single-batch measurement is reduced, and the measurement time difference among a plurality of batches is small; the invention does not need manual measurement for multiple times and multiple samples one by one at high temperature, can automatically complete circular continuous measurement, saves time and labor, reduces errors of manual measurement, and improves the accuracy and stability of photosynthetic parameter measurement.
Drawings
FIGS. 1 and 2 are schematic views showing the overall structure of the cyclic measurement device from different viewing angles.
FIG. 3 is a side view of the cycle measuring apparatus.
FIG. 4 is a plan view of the circulation measuring apparatus.
Fig. 5 is a sectional view taken along line a-a in fig. 4.
The device comprises a base plate 1, a vertical column 11, spokes 2, arc-shaped blocks 21, mounting blocks 22, vertical plates 23, a rotating shaft 24, a gear II 25, a concave frame 26, an adsorption groove 261, an annular sliding rail 3, a sliding chute 31, trapezoidal protrusions 32, an annular gear 33, a motor I4, a gear I41, a transverse roller 5, a lifting rod 51, a spring I52, a rack 53, a leaf chamber 6, a pressing handle 61, a handle 62, a spring II 63, a pressing frame 64, a pressing rod 65, a top plate 7, a connecting rod 71, a rotating block 72, a sliding block 73, a motor II 74, a screw 75 and a motor III 8.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
Example 1: a method for detecting the high-temperature stability of the photosynthetic capacity of rice comprises the following steps:
step one, installing a portable photosynthetic determinator on a cycle determination device, and placing the cycle determination device in an artificial climate chamber for later use; the portable photosynthetic rate tester adopts an external carbon dioxide system;
step two, transplanting rice plants into a pot for planting, after carrying out adaptive culture in an artificial climate chamber for 7 days, placing a plurality of rice plants to be detected outside a circular surrounding manner in a circulation determination device, sticking magnetic sheets at the upper end and the lower end of the back of the sword leaves of the single rice plant, adsorbing the sword leaves on the upper part of a leaf pressing mechanism through the magnetic sheets, and completely drawing out and unfolding the sword leaves of the rice plants to be detected; the illumination intensity of the artificial climate chamber is 16000Lx, the temperature is 25 ℃, the humidity is 60-70%, and the illumination is 14 h/d;
step three, continuously measuring the photosynthetic rate at high temperature: only adjusting the temperature parameter of the artificial climate chamber to 39 ℃, measuring the photosynthetic rate and stomatal conductance of the flag leaves of different rice materials every 2 hours from 9 o 'clock to 17 o' clock, repeatedly measuring at least 10 plants of each rice material, and selecting the same part of each rice flag leaf to carry out multiple measurements during measurement, wherein the measured part is the middle part of the flag leaf; light and thin magnetic sheets are respectively adhered to the upper end and the lower end of the back of the sword leaf, potted rice is correspondingly placed on the outer side of the concave frame 26 one by one, the magnetic sheets are adsorbed in the adsorption groove 261, the sword leaf is adsorbed and flattened by the magnetic sheets and fixed on the concave frame 26, the motor I4 and the motor III 8 are turned on, the motor I4 drives the gear I41 to rotate, the gear I41 drives the annular slide rail 3 to rotate through the inner gear ring 33, the motor III 8 drives the top plate 7 to rotate, the top plate 7 drives the connecting rod 71 and the rotating block 72 to rotate, the rotating block 72 drives the measuring mechanism on the rotating block to rotate around the upright post 11, the angular speed of the rotating block 72 is kept to be the same as the angular speed of the annular slide rail 3, the trapezoidal projections 32 and the rotating block are synchronously rotated, the trapezoidal projections 32 are kept to be positioned in the advancing direction of the rotating block 72 when the rotating block is synchronously rotated, namely the trapezoidal projections 32 are positioned in front of the rotating block 72 to ensure that the leaf chamber 6 can be forwardly extended, the transverse roller 5 can be pressed downwards in advance by the trapezoidal projections 32, when the trapezoidal protrusion 32 passes through the horizontal roller 5, the horizontal roller 5 moves downwards until the horizontal roller 5 rolls to the bottom plane of the trapezoidal protrusion 32, at this time, the lifting rod 51 moves downwards to reach the maximum stroke, the lifting rod 51 moves downwards to pull the rack 53, the rack 53 pulls the second gear 25 to rotate, the second gear 25 rotates together with the rotating shaft 24, the rotating shaft 24 drives the concave frame 26 to rotate until the concave frame 26 is horizontally arranged in front of the leaf chamber 6, the first motor 4 and the third motor 8 stop at the same time, the concave frame 26 keeps in a horizontal state, at this time, the leaf chamber 6 stays behind the opening of the concave frame 26 and does not extend into the concave frame 26, the leaf chamber 6 is in an open state, the second motor 74 is turned, the second motor 74 rotates the screw 75, the screw 75 drives the slider 73 to move forwards, the slider 73 drives the grab handle 62 and the leaf chamber 6 to move forwards, the leaf chamber 6 drives the press handle 61 and the press rod 65 to move forwards, and two ends of the press rod 65 roll forwards along the lower edge of the press frame 64, until the blade rotates to the wedge-shaped inclined plane of the pressing frame 64, the second spring 63 pushes the handle 61 upwards, the pressing rod 65 is lifted along the inclined plane, so that the upper part of the blade chamber 6 is driven by the front end of the pressing handle 61 to be pressed downwards until the blade chamber 6 is clamped, and when the sliding block 73 moves forwards to the maximum stroke, the blade chamber 6 just extends into the concave frame 26 and clamps the sword blades on the concave frame 26 to measure the photosynthetic parameters. After the measurement is finished, the second motor 74 is reversed, the sliding block 73 is retracted, the pressing rod 65 is pressed down by the wedge-shaped inclined plane in the process of retracting the vane chamber 6, the second spring 63 is compressed, the rear end of the pressing handle 61 descends, the front end of the pressing handle 61 drives the upper part of the vane chamber 6 to be opened, the vane is prevented from being clamped and not placed in the retracting process of the vane chamber 6, the vane is broken, and when the vane chamber 6 retracts and exits the concave frame 26, the second motor 74 is closed. At this time, the measuring process is finished, the first motor 4 and the second motor 74 are simultaneously turned on, the trapezoidal protrusion 32, the rotating block 72 and the annular slide rail 3 continue to synchronously rotate, at this time, the transverse roller 5 rolls and moves upwards along the rear inclined surface of the trapezoidal protrusion 32, the lifting rod 51 slides upwards under the action of the elastic force of the first spring 52, the lifting rod 51 pushes the rack 53 upwards, the rack 53 drives the gear to reversely rotate, the concave frame 26 reversely rotate and lift up around the rotating shaft 24 to allow the leaf chamber 6 to rotate, the measuring mechanism and the rotating block 72 continue to rotate until the trapezoidal protrusion 32 meets the transverse roller 5 on the next station, and when the next transverse roller 5 rolls to the bottom plane of the trapezoidal protrusion 32, the measuring process is repeated. Thus, continuous multi-sample circulation measurement can be completed.
And step four, the measured photosynthetic rate parameters are led into a computer for processing and analysis, and then the stability of the photosynthetic capacity of the rice plant under the high-temperature condition can be obtained.
The structure of the circulation measuring device adopted in the invention is shown in figures 1 to 5, and comprises a bottom plate 1, wherein a vertical column 11 is arranged in the center of the bottom plate 1, a plurality of spokes 2 are uniformly arranged at the upper part of the vertical column 11 along the radial direction, arc-shaped blocks 21 are arranged at the far ends of the spokes 2, an annular slide rail 3 is arranged below the arc-shaped blocks 21, a slide groove 31 is arranged on the annular slide rail 3, the arc-shaped blocks 21 are all slidably connected in the slide groove 31, a trapezoidal protrusion 32 is arranged at the bottom of the annular slide rail 3, an inner gear ring 33 is arranged at the inner side of the annular slide rail 3, a motor I4 is arranged on the bottom plate 1, a gear I41 is arranged on an output shaft of the motor I4, the gear I41 is meshed with the inner gear ring 33, leaf pressing mechanisms are arranged on the arc-shaped blocks 21, the leaf pressing mechanisms are sleeved outside the annular slide rail 3, a motor III 8 is arranged at the top of the vertical column 11, and a top end of the vertical column 11 is coaxially and rotatably connected with a top plate 7, the bottom surface of the top plate 7 is connected with an output shaft of the motor III 8, a connecting rod 71 is radially arranged on the outer side of the top plate 7 along the top plate 7, a rotating block 72 is arranged at the far end of the connecting rod 71, a telescopic mechanism and a pressing frame 64 are arranged on the rotating block 72, a measuring mechanism is arranged on the telescopic mechanism, the measuring mechanism is controlled by the pressing frame 64 to be pressed or reset and opened in the telescopic process, the adsorbed blades are transversely arranged in front of an advancing path of the measuring mechanism by the pressing mechanism when the trapezoidal protrusions 32 pass through the pressing mechanism, and the pressing mechanism is reset and lifted when the trapezoidal protrusions 32 are separated from the pressing mechanism.
In this embodiment, the leaf pressing mechanism includes an installation block 22 disposed on an arc block 21, the installation block 22 is slidably connected with two lifting rods 51, the two lifting rods 51 are symmetrically disposed inside and outside the annular slide rail 3, a cross roller 5 is disposed between lower ends of the two lifting rods 51, the cross roller 5 is disposed below the annular slide rail 3, a first spring 52 is disposed between each of two ends of the cross roller 5 and the installation block 22, the first spring 52 is sleeved outside the lifting rod 51, when the trapezoidal bump passes through the position of the cross roller 5, the cross roller 5 is in rolling contact with the bottom surface of the trapezoidal bump, a rack 53 is disposed at the upper end of the lifting rod 51 located outside the annular slide rail 3, a vertical plate 23 is disposed on one side of the installation block 22, the vertical plate 23 is disposed outside the annular slide rail 3, a rotating shaft 24 is rotatably connected to the top of the vertical plate 23, one end of the rotating shaft 24 is provided with a second gear 25, the second gear 25 is engaged with the rack 53, the middle part of the rotating shaft 24 is provided with a concave frame 26 along the radial direction, the concave frame 26 is provided with an adsorption groove 261, and when the concave frame 26 is transversely arranged in front of the measuring mechanism, the measuring mechanism extends forwards to enter a gap of the concave frame 26 to clamp the blade for measurement.
In this embodiment, measuring mechanism includes leaf chamber 6, grab handle 62 and pressure handle 61, be equipped with two springs 63 between pressure handle 61 and the grab handle 62, grab handle 62 is located telescopic machanism, the front end of grab handle 62 is located to leaf chamber 6, the front end of pressing handle 61 is connected with the upper portion of leaf chamber 6, the rear end of pressing handle 61 is equipped with depression bar 65, the both ends of depression bar 65 roll the butt with pressure frame 64, the front end of pressing frame 64 is the wedge, leaf chamber 6 passes through the hose and the electric wire is connected with the host computer, the host computer is located on roof 7.
In this embodiment, the telescopic mechanism includes a slider 73 disposed below the handle 62, the bottom of the slider 73 is slidably connected to a rotating block 72, a screw 75 is rotatably connected to the rotating block 72, the screw 75 is in threaded connection with the slider 73, a second motor 74 is disposed at the rear end of the rotating block 72, and an output shaft of the second motor 74 is connected to an end of the screw 75.
As shown in fig. 1, the telescopic mechanism and the measuring mechanism can be added to 2 or more groups in a mirror image manner according to needs, more rice samples can be measured at the same time when the rice sample rotates once, and time consumption for parameter measurement can be greatly reduced.
Comparative example: the rest of the procedure was the same as in example 1, except that the rice flag leaves were measured one by using a manually operated portable photosynthetic rate measuring instrument.
The time taken for a single measurement of example 1 and the control example was recorded for a total of 6 times, and the comparison results are shown in table 1:
TABLE 1 comparison of the elapsed time for a single measurement of a full sample
| Group of | Time consumed for a single measurement (min) | Measurement of Total number of samples (Strain) |
| Example 1 | 31.4±1.3 | 100 |
| Comparative example | 113.2±33.9 | 100 |
As can be seen from the results in table 1, in the manual measurement, the SD value of a single measurement is large, the operation skill of the measuring staff and the body state at high temperature directly affect the measurement time, which results in large time consumption fluctuation of the multi-batch measurement, and is not favorable for maintaining the corresponding relationship between the parameter data and the time, and the time difference is inversely proportional to the data comparability, and is not favorable for maintaining the stability and accuracy of the data. On the contrary, the method can effectively reduce the time consumption of single measurement, has small time consumption fluctuation of multi-batch measurement, and effectively overcomes the defect of manual measurement.
It will be appreciated by those skilled in the art that the invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The embodiments disclosed above are therefore to be considered in all respects as illustrative and not restrictive. All changes which come within the scope of or equivalence to the invention are intended to be embraced therein.
Claims (5)
1. A method for detecting the high-temperature stability of the photosynthetic capacity of rice is characterized by comprising the following steps:
step one, installing a portable photosynthetic determinator on a cycle determination device, and placing the cycle determination device in an artificial climate chamber for later use; the portable photosynthetic rate tester adopts an external carbon dioxide system;
step two, transplanting rice plants into a pot for planting, after carrying out adaptive culture in an artificial climate chamber for 7 days, placing a plurality of rice plants to be detected outside a circular surrounding manner in a circulation determination device, sticking magnetic sheets at the upper end and the lower end of the back of the sword leaves of the single rice plant, adsorbing the sword leaves on the upper part of a leaf pressing mechanism through the magnetic sheets, and completely drawing out and unfolding the sword leaves of the rice plants to be detected; the illumination intensity of the artificial climate chamber is 16000Lx, the temperature is 25 ℃, the humidity is 60-70%, and the illumination is 14 h/d;
step three, continuously measuring the photosynthetic rate at high temperature: only adjusting the temperature parameter of the artificial climate chamber to 39 ℃, measuring the photosynthetic rate and stomatal conductance of the flag leaves of different rice materials every 2 hours from 9 o 'clock to 17 o' clock, repeatedly measuring at least 10 plants of each rice material, and selecting the same part of each rice flag leaf to carry out multiple measurements during measurement, wherein the measured part is the middle part of the flag leaf; the portable photosynthetic apparatus is controlled by the circulation measuring device to make intermittent circular rotation, the rice plants surrounding the portable photosynthetic apparatus are measured one by one in the rotation process, the rotation is stopped when the portable photosynthetic apparatus is close to the rice plant to be measured, the circulation measuring device presses down a single sword leaf to be horizontally arranged in front of a leaf chamber of the portable photosynthetic apparatus, the circulation measuring device controls the leaf chamber to extend forwards and press and measure the photosynthetic parameters of the sword leaf, after the measurement, the leaf chamber is retracted and separated from the leaf for resetting, the sword leaf is lifted by the circulation measuring device to give way for the portable photosynthetic apparatus, the portable photosynthetic apparatus continuously rotates until the sword leaf is close to the next rice plant to be measured, and the detection steps are repeated to continuously measure the photosynthetic rate parameters of the surrounding rice plant one by one;
and step four, the measured photosynthetic rate parameters are led into a computer for processing and analysis, and then the photosynthetic capacity of the rice plant under the high-temperature condition can be obtained.
2. The method for detecting the high-temperature stability of the photosynthetic capacity of rice according to claim 1, wherein the circulation measuring device comprises a bottom plate (1), a vertical column (11) is arranged in the center of the bottom plate (1), a plurality of spokes (2) are uniformly arranged on the upper portion of the vertical column (11) along the radial direction, arc-shaped blocks (21) are arranged at the far ends of the spokes (2), an annular slide rail (3) is arranged below the arc-shaped blocks (21), a slide groove (31) is arranged on the annular slide rail (3), the arc-shaped blocks (21) are slidably connected in the slide groove (31), trapezoidal protrusions (32) are arranged at the bottom of the annular slide rail (3), an inner gear ring (33) is arranged on the inner side of the annular slide rail (3), a first motor (4) is arranged on the bottom plate (1), a first gear (41) is arranged on an output shaft of the first motor (4), and the first gear (41) is meshed with the inner gear ring (33), the leaf pressing mechanisms are arranged on the arc-shaped blocks (21) and are sleeved outside the annular sliding rail (3), a third motor (8) is arranged at the top of the upright post (11), the top end of the upright post (11) is coaxially and rotatably connected with a top plate (7), the bottom surface of the top plate (7) is connected with an output shaft of the third motor (8), a connecting rod (71) is radially arranged on the outer side of the top plate (7) along the top plate (7), a rotating block (72) is arranged at the far end of the connecting rod (71), a telescopic mechanism and a pressing frame (64) are arranged on the rotating block (72), a measuring mechanism is arranged on the telescopic mechanism, the measuring mechanism is controlled by the pressing frame (64) to be pressed or reset and opened in the telescopic process, the leaf pressing mechanism horizontally arranges the adsorbed leaves in front of the advancing path of the measuring mechanism when the trapezoidal bulge (32) passes through the leaf pressing mechanism, and when the trapezoidal bulge (32) is separated from the leaf pressing mechanism, the leaf pressing mechanism resets and lifts.
3. The method for detecting the high-temperature stability of the photosynthetic capacity of rice according to claim 2, wherein the leaf pressing mechanism comprises a mounting block (22) arranged on an arc-shaped block (21), the mounting block (22) is connected with two lifting rods (51) in a sliding manner, the two lifting rods (51) are symmetrically arranged inside and outside the annular slide rail (3), a transverse roller (5) is arranged between the lower ends of the two lifting rods (51), the transverse roller (5) is arranged below the annular slide rail (3), a first spring (52) is arranged between each of the two ends of the transverse roller (5) and the mounting block (22), the first spring (52) is sleeved outside the lifting rods (51), when the trapezoidal convex block passes through the position of the transverse roller (5), the transverse roller (5) rolls against the bottom surface of the trapezoidal convex block, a rack (53) is arranged at the upper end of the lifting rod (51) positioned outside the annular slide rail (3), one side of installation piece (22) is equipped with riser (23), the annular slide rail (3) outside is located in riser (23), the top of riser (23) is rotated and is connected with pivot (24), the one end of pivot (24) is equipped with gear two (25), gear two (25) and rack (53) meshing, pivot (24) middle part is along radially being equipped with concave frame (26), be equipped with on concave frame (26) and adsorb groove (261), when concave frame (26) transversely was arranged in measuring mechanism the place ahead, measuring mechanism stretched forward and got into the breach internal clamping blade of concave frame (26) and measure.
4. The method for detecting the high-temperature stability of the photosynthetic capacity of rice of claim 3, wherein the measuring mechanism comprises a leaf chamber (6), a grab handle (62) and a press handle (61), a second spring (63) is arranged between the press handle (61) and the grab handle (62), the grab handle (62) is arranged on the telescopic mechanism, the leaf chamber (6) is arranged at the front end of the grab handle (62), the front end of the press handle (61) is connected with the upper part of the leaf chamber (6), the rear end of the press handle (61) is provided with a press rod (65), two ends of the press rod (65) are in rolling contact with a press frame (64), the front end of the press frame (64) is in a wedge shape, the leaf chamber (6) is connected with a host machine through a hose and an electric wire, and the host machine is arranged on the top plate (7).
5. The method for detecting the high-temperature stability of the photosynthetic capacity of rice as claimed in claim 4, wherein the telescoping mechanism comprises a sliding block (73) arranged below the handle (62), the bottom of the sliding block (73) is slidably connected with a rotating block (72), a screw rod (75) is rotatably connected in the rotating block (72), the screw rod (75) is in threaded connection with the sliding block (73), a second motor (74) is arranged at the rear end of the rotating block (72), and an output shaft of the second motor (74) is connected with the end of the screw rod (75).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111232821.4A CN114002382A (en) | 2021-10-22 | 2021-10-22 | Method for detecting high-temperature stability of rice photosynthetic capacity |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111232821.4A CN114002382A (en) | 2021-10-22 | 2021-10-22 | Method for detecting high-temperature stability of rice photosynthetic capacity |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114451297A (en) * | 2022-03-02 | 2022-05-10 | 金华职业技术学院 | Auxiliary device for artificial pollination of rice |
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2021
- 2021-10-22 CN CN202111232821.4A patent/CN114002382A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114451297A (en) * | 2022-03-02 | 2022-05-10 | 金华职业技术学院 | Auxiliary device for artificial pollination of rice |
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Effective date of registration: 20220322 Address after: 241000 building A12, outsourcing park of high tech service industry, Wuhu Development Zone, Anhui Province Applicant after: Anhui Yuanliang Seed Industry Research Institute Applicant after: YIELEAD RICE INDUSTRY CO.,LTD. Address before: 241000 building A12, Service Outsourcing Industrial Park, Wuhu high tech Industrial Development Zone, Anhui Province (No. 717, south Zhongshan Road) Applicant before: YIELEAD RICE INDUSTRY CO.,LTD. |
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Application publication date: 20220201 |