CN113999926A - Primer group, kit and application of kit in rapid identification of xanthoxylum - Google Patents
Primer group, kit and application of kit in rapid identification of xanthoxylum Download PDFInfo
- Publication number
- CN113999926A CN113999926A CN202110206459.7A CN202110206459A CN113999926A CN 113999926 A CN113999926 A CN 113999926A CN 202110206459 A CN202110206459 A CN 202110206459A CN 113999926 A CN113999926 A CN 113999926A
- Authority
- CN
- China
- Prior art keywords
- primer
- pcr amplification
- primer group
- fusarium solani
- kit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Mycology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to the technical field of molecular detection, and discloses a primer group and a kit containing the primer group; the primer group comprises an upstream primer F1 and a downstream primer R1, and the sequences are as follows: the upstream primer F1: 5'-CGTGTCGCAGAAGAGGG-3', respectively; the downstream primer R1: 5'-AATAGGAAGCCGCTGAGC-3', respectively; the invention also discloses application of the primer group or the kit in rapid identification of the xanthoxylum rot germs, which comprises the following steps: s1, extracting sample DNA; s2, carrying out PCR amplification reaction by using the sample DNA as a template and using the primer group to obtain a PCR amplification product; s3, analyzing a PCR amplification product; the primer group can specifically identify fusarium solani, and has the characteristics of high amplification efficiency, strong specificity and high accuracy; meanwhile, the rapid identification method established by combining the primer group is quite simple, and fusarium solani can be identified without morphological identification and DNA sequencing.
Description
Technical Field
The invention relates to the technical field of molecular detection, in particular to a primer group, a kit and application thereof in rapid identification of xanthoxylum.
Background
Zanthoxylum bungeanum (Zanthoxylum bungeanum Maxim) is a small deciduous tree of Rutaceae and Zanthoxylum genus, and has drought resistance and sunlight preference, and is widely cultivated in various fields. The peel of fructus Zanthoxyli can be used as flavoring agent, and can be used for extracting aromatic oil, or used as medicine, and the seed can be used for edible purpose, or processed into soap.
Root rot is a disease caused by fungi, which causes root rot, gradually weakens the function of absorbing water and nutrients, and finally dies the whole plant, mainly manifested as yellowing and withering of the whole plant leaves. The Chinese prickly ash root rot is a soil-borne disease mainly caused by fusarium solani, which often occurs in nurseries and adult Chinese prickly ash fruit tree gardens, the roots of damaged plants are discolored and rotten and have peculiar smell, the root bark is separated from xylem, and the xylem is black; the leaves on the ground are small and yellow, the branches are not completely developed, and the whole plant dies when the branches are serious. The disease can cause that the old pepper garden can not continuously plant the pepper, and seriously restricts the healthy and sustainable development of the pepper industry.
At present, the method for identifying the fusarium solani is mainly to carry out disinfection treatment and pathogenic bacteria separation on root tissues of infected plants, carry out indoor in-vitro culture after obtaining purified strains, and observe morphological characteristics such as shapes, sizes and the like of spores to determine that the pathogen is fusarium solani. However, because fusarium species are various, some fusarium species have multiple specialization types, and morphological characteristics between species are relatively close, and identification of fusarium solani only by morphological characteristics is not only complex in operation method, but also not accurate in identification result.
Therefore, a method for rapidly identifying fusarium solani in pepper with simple operation, high accuracy and high specificity is needed.
Disclosure of Invention
One of the purposes of the invention is to overcome the defects of the prior art and provide a primer group to at least achieve the effects of simple operation, high accuracy and high specificity, and the primer group is suitable for identifying fusarium solani in pepper.
The above purpose is realized by the following technical scheme: a primer group, comprising an upstream primer F1 and a downstream primer R1, wherein the sequences of the primer group are as follows:
the upstream primer F1: 5'-CGTGTCGCAGAAGAGGG-3', respectively;
the downstream primer R1: 5'-AATAGGAAGCCGCTGAGC-3' are provided.
The second object of the present invention is to provide a kit comprising the primer set.
The invention also aims to provide the application of the primer group or the kit in the rapid identification of the xanthorrhiza rot fungi.
In certain embodiments, the xanthorrhiza species is fusarium solani.
In certain embodiments, the method for rapidly identifying fusarium solani comprises the steps of:
s1, extracting sample DNA;
s2, carrying out PCR amplification reaction by using the sample DNA as a template and using the primer group to obtain a PCR amplification product;
and S3, analyzing the PCR amplification product.
In certain embodiments, the reaction system of the PCR amplification reaction is, on a 25 μ L basis:
in certain embodiments, the concentration of both the forward primer F1 and the reverse primer R1 is 10. mu. mol/L.
In certain embodiments, the concentration of the sample DNA is 50 ng/. mu.L.
In certain embodiments, the reaction conditions of the PCR amplification reaction are: pre-denaturation at 98 ℃ for 2min, denaturation at 98 ℃ for 15s, annealing at 58 ℃ for 15s, extension at 72 ℃ for 30min, and 35 cycles; stop at 72 ℃ for 5 min.
In some embodiments, the analyzing the PCR amplification product is detecting the PCR amplification product by agarose gel electrophoresis, and if a target band with a size of 210bp appears, fusarium solani exists; if the target band with the size of 210bp does not appear, fusarium solani does not exist.
It is worth noting that the primer set can specifically identify the fusarium solani without being influenced by fusarium oxysporum, fusarium graminearum, fusarium moniliforme and the like with relatively similar morphological characteristics, and achieves the characteristics of high amplification efficiency, strong specificity and high accuracy. Meanwhile, the invention establishes a method for rapidly identifying fusarium solani by combining the primer group, and the primer group is combined with the sample DNA, the Taq Mix and the ddH2Preparing a PCR reaction system by using O, and carrying out conventional PCR detection; the method breaks through the traditional identification method, so that the identification process is quite simple, sequencing is not needed, only the conventional PCR reaction solution is subjected to DNA electrophoresis, and whether the common PCR reaction solution is a 210 bp-sized strip or not can be observed to determine whether the common PCR reaction solution is the xanthoxylum solani (fusarium solani), an accurate detection result can be obtained within 7 hours, and compared with the traditional microbiological method, the method has the advantages that the time required by identification is remarkably shortened by more than 2-7 days.
The invention has the beneficial effects that:
1. the primer group disclosed by the invention has the advantages of high amplification efficiency, strong specificity and high accuracy, and a practical, simple and convenient method is provided for identifying the Fusarium solani by combining the rapid identification method established by the primer group.
2. The invention breaks through the traditional identification method, so that the identification process is quite simple, and the identification of the xanthoxylum (fusarium solani) can be carried out without morphological identification and DNA sequencing.
3. The method can detect the pepper root rot in early stage, advances the prevention and treatment period, improves the prevention effect, and has important significance for early finding and early treatment of the pepper root rot.
4. The identification method can obtain an accurate detection result within 7 hours, and compared with the traditional microbiological method for more than 2-7 days, the identification method obviously shortens the time required by identification.
Drawings
FIG. 1 is a photograph showing agarose gel electrophoresis detection of each set of PCR amplification products in example 1 of the present invention;
wherein the strips are 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 and 13 from left to right; the strip 1 is a marker and sequentially comprises 2000bp, 1000bp, 750bp, 500bp, 250bp and 100bp from top to bottom; the samples of the strips 2, 4, 5, 6, 7 are fusarium solani; the samples of the strips 3 and 8 are fusarium oxysporum; the sample of the strips 9, 10 is fusarium graminearum; the sample of the strips 11, 12 is fusarium moniliforme; the sample of strip 13 is Botrytis cinerea;
FIG. 2 is an agarose gel electrophoresis test chart of each PCR amplification product set in examples 2-3 of the present invention;
wherein the strips are 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23 and 24 in turn from left to right; the strip 1 is a marker and sequentially comprises 2000bp, 1000bp, 750bp, 500bp, 250bp and 100bp from top to bottom; the sample of the strip 2-8 is rhizosphere soil of the diseased pepper roots inoculated with fusarium solani; the sample of the strip 9-17 is the diseased pepper roots inoculated with fusarium solani; the sample of the strip 18-24 is healthy pepper roots which are not inoculated with fusarium solani.
Detailed Description
The technical solutions of the present invention are further described in detail below with reference to the accompanying drawings, but the scope of the present invention is not limited to the following.
Example 1
A method for rapidly identifying xanthoxylum bungeanum (fusarium solani) comprises the following steps:
s1, synthesizing a primer group: the primer group comprises an upstream primer F1 and a downstream primer R1; the sequence of the upstream primer F1 is as follows: 5'-CGTGTCGCAGAAGAGGG-3', the sequence of the downstream primer R1 is: 5'-AATAGGAAGCCGCTGAGC-3', respectively; wherein the synthesis concentration of the upstream primer F1 and the downstream primer R1 is 10 mu mol/L;
s2, extracting sample DNA: extracting DNA diluent with the concentration of 50 ng/mu L from fusarium solani, fusarium oxysporum, fusarium graminearum, fusarium moniliforme and botrytis cinerea respectively by adopting a conventional DNA extraction means (CTAB method) to obtain a plurality of groups of sample DNA;
s3, preparing a PCR reaction system: adding each group of sample DNA into a respective 25 mu L reaction system, wherein each 25 mu L reaction system comprises the following components:
s4, PCR conventional detection: according to the PCR reaction system, taking each group of sample DNA as a template, and respectively carrying out PCR amplification on the sample DNA by utilizing a primer group to obtain each group of PCR amplification products; wherein, the PCR amplification adopts a conventional BIO-RAD PCR instrument; the PCR reaction conditions are as follows: pre-denaturation at 98 ℃ for 2min, denaturation at 98 ℃ for 15s, annealing at 58 ℃ for 15s, extension at 72 ℃ for 30min, and 35 cycles; stopping at 72 ℃ for 5 min;
s5, analyzing PCR amplification products: taking 8 mu L of each PCR amplification product, respectively adding 1 mu L of sample loading buffer solution, carrying out electrophoresis in 1.5% (w/V) agarose gel at 10V/cm for 30min, staining with Ethidium Bromide (EB), and imaging on a gel phase formation system after 10 min;
s6, judging a result: and (3) placing the agarose gel under ultraviolet irradiation to judge whether a target band with the size of 210bp exists in each group.
The result is shown in fig. 1, and the bands are 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 and 13 from left to right; wherein, the strip 1 is a marker which is 2000bp, 1000bp, 750bp, 500bp, 250bp and 100bp sequentially from top to bottom; the samples of the bands 2, 4, 5, 6 and 7 are fusarium solani, and the target band of 210bp can be amplified; the samples of the bands 3 and 8 are fusarium oxysporum, the samples of the bands 9 and 10 are fusarium graminearum, the samples of the bands 11 and 12 are fusarium moniliforme, the sample of the band 13 is botrytis cinerea, and the target bands of 210bp are not amplified. Therefore, the primer group of the invention has strong specificity and high accuracy.
Example 2
A method for rapidly identifying xanthoxylum bungeanum (fusarium solani) comprises the following steps:
s1, synthesizing a primer group: the primer group comprises an upstream primer F1 and a downstream primer R1; the sequence of the upstream primer F1 is as follows: 5'-CGTGTCGCAGAAGAGGG-3', the sequence of the downstream primer R1 is: 5'-AATAGGAAGCCGCTGAGC-3', respectively; wherein the synthesis concentration of the upstream primer F1 and the downstream primer R1 is 10 mu mol/L;
s2, extracting sample DNA: extracting DNA diluent with the concentration of 50 ng/mu L from the rhizosphere soil of the ill pepper roots inoculated with fusarium solani by adopting a conventional DNA extraction means (CTAB method) to obtain sample DNA;
s3, preparing a PCR reaction system: the sample DNA was added to a 25. mu.L reaction system comprising the following components:
s4, PCR conventional detection: according to the PCR reaction system, taking sample DNA as a template, and performing PCR amplification by using a primer group to obtain a PCR amplification product; wherein, the PCR amplification adopts a conventional BIO-RAD PCR instrument; the PCR reaction conditions are as follows: pre-denaturation at 98 ℃ for 2min, denaturation at 98 ℃ for 15s, annealing at 58 ℃ for 15s, extension at 72 ℃ for 30min, and 35 cycles; stopping at 72 ℃ for 5 min;
s5, analyzing PCR amplification products: adding 1 μ L sample buffer solution into 8 μ L LPCR amplification product, performing electrophoresis in 1.5% (w/V) agarose gel at 10V/cm for 30min, staining with Ethidium Bromide (EB), and imaging on gel phase system after 10 min;
s6, judging a result: and (3) placing the agarose gel under ultraviolet irradiation to judge whether a target band with the size of 210bp exists.
The result is shown in fig. 2, the bands are 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23 and 24 from left to right; wherein, the strip 1 is a marker which is 2000bp, 1000bp, 750bp, 500bp, 250bp and 100bp sequentially from top to bottom; the samples with the bands of 2-8 are rhizosphere soil of the diseased pepper roots inoculated with fusarium solani, and the target bands of 210bp can be amplified. Therefore, the primer group can detect fusarium solani from the soil around the roots of the pepper root rot disease.
Example 3
A method for rapidly identifying xanthoxylum bungeanum (fusarium solani) comprises the following steps:
s1, synthesizing a primer group: the primer group comprises an upstream primer F1 and a downstream primer R1; the sequence of the upstream primer F1 is as follows: 5'-CGTGTCGCAGAAGAGGG-3', the sequence of the downstream primer R1 is: 5'-AATAGGAAGCCGCTGAGC-3', respectively; wherein the synthesis concentration of the upstream primer F1 and the downstream primer R1 is 10 mu mol/L;
s2, extracting sample DNA: extracting DNA diluent with concentration of 50 ng/mu L from healthy pepper roots which are not inoculated with fusarium solani and diseased pepper roots which are inoculated with fusarium solani respectively by adopting a conventional DNA extraction means (CTAB method) to obtain 2 groups of sample DNA;
s3, preparing a PCR reaction system: 2 groups of sample DNAs were added to respective 25. mu.L reaction systems, each 25. mu.L reaction system comprising the following components:
s4, PCR conventional detection: according to the PCR reaction system, 2 groups of sample DNAs are respectively used as templates, and PCR amplification is carried out on the samples by utilizing primer groups to obtain 2 groups of PCR amplification products; wherein, the PCR amplification adopts a conventional BIO-RAD PCR instrument; the PCR reaction conditions are as follows: pre-denaturation at 98 ℃ for 2min, denaturation at 98 ℃ for 15s, annealing at 58 ℃ for 15s, extension at 72 ℃ for 30min, and 35 cycles; stopping at 72 ℃ for 5 min;
s5, analyzing PCR amplification products: taking 8 mu L of each PCR amplification product of 2 groups, respectively adding 1 mu L of loading buffer solution, carrying out electrophoresis in 1.5% (w/V) agarose gel at 10V/cm for 30min, staining with Ethidium Bromide (EB), and imaging on a gel phase formation system after 10 min;
s6, judging a result: and (3) placing the agarose gel under ultraviolet irradiation to judge whether a target band with the size of 210bp exists in each group.
The result is shown in fig. 2, the bands are 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23 and 24 from left to right; wherein, the strip 1 is a marker which is 2000bp, 1000bp, 750bp, 500bp, 250bp and 100bp sequentially from top to bottom; samples of the bands 9-17 are diseased pepper roots inoculated with fusarium solani, and target bands of 210bp can be amplified; samples with the bands of 18-24 are healthy pepper roots which are not inoculated with fusarium solani, and 210bp target bands are not amplified. Therefore, the primer group can detect fusarium solani from the infected pepper roots, and healthy pepper roots without inoculation cannot detect the target fragment.
In conclusion, the primer group is suitable for identifying fusarium solani in pepper, and has strong accuracy and specificity; the rapid identification method established by combining the primer group provides a practical and simple method for identifying the fusarium solani, so that the identification process is quite simple, and the rhizoctonia solani (fusarium solani) can be identified without morphological identification and DNA sequencing.
The foregoing is illustrative of the preferred embodiments of this invention, and it is to be understood that the invention is not limited to the precise form disclosed herein and that various other combinations, modifications, and environments may be resorted to, falling within the scope of the concept as disclosed herein, either as described above or as apparent to those skilled in the relevant art. And that modifications and variations may be effected by those skilled in the art without departing from the spirit and scope of the invention as defined by the appended claims.
Sequence listing
<110> institute of plant protection of academy of agricultural sciences of Sichuan province
<120> primer group, kit and application thereof in rapid identification of xanthoxylum
<160> 2
<170> SIPOSequenceListing 1.0
<210> 1
<211> 17
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 1
<210> 2
<211> 18
<212> DNA
<213> Artificial Sequence (Artificial Sequence)
<400> 2
Claims (10)
1. A primer group is characterized by comprising an upstream primer F1 and a downstream primer R1, and the sequences are as follows:
the upstream primer F1: 5'-CGTGTCGCAGAAGAGGG-3', respectively;
the downstream primer R1: 5'-AATAGGAAGCCGCTGAGC-3' are provided.
2. A kit comprising the primer set of claim 1.
3. The primer set of claim 1 or the kit of claim 2, for use in rapid identification of xanthorrhiza rot disease.
4. The use according to claim 3, wherein the Rhizopus zanthoxyli is Fusarium solani.
5. The use according to claim 4, wherein the method for rapidly identifying Fusarium solani comprises the steps of:
s1, extracting sample DNA;
s2, carrying out PCR amplification reaction by using the sample DNA as a template and using the primer group to obtain a PCR amplification product;
and S3, analyzing the PCR amplification product.
6. The use of claim 5, wherein the reaction system of the PCR amplification reaction is as follows, in 25 μ L:
7. the use of claim 6, wherein the concentration of the forward primer F1 and the concentration of the backward primer R1 are both 10 μmol/L.
8. The use of claim 6, wherein the sample DNA is at a concentration of 50ng/μ L.
9. The use according to claim 5, wherein the reaction conditions of the PCR amplification reaction are: pre-denaturation at 98 ℃ for 2min, denaturation at 98 ℃ for 15s, annealing at 58 ℃ for 15s, extension at 72 ℃ for 30min, and 35 cycles; stop at 72 ℃ for 5 min.
10. The use of claim 5, wherein the analysis of the PCR amplification product is performed by agarose gel electrophoresis detection of the PCR amplification product, and if a target band with a size of 210bp is present, Fusarium solani is present; if the target band with the size of 210bp does not appear, fusarium solani does not exist.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110206459.7A CN113999926A (en) | 2021-02-24 | 2021-02-24 | Primer group, kit and application of kit in rapid identification of xanthoxylum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110206459.7A CN113999926A (en) | 2021-02-24 | 2021-02-24 | Primer group, kit and application of kit in rapid identification of xanthoxylum |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113999926A true CN113999926A (en) | 2022-02-01 |
Family
ID=79920893
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110206459.7A Withdrawn CN113999926A (en) | 2021-02-24 | 2021-02-24 | Primer group, kit and application of kit in rapid identification of xanthoxylum |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113999926A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108841985A (en) * | 2018-06-29 | 2018-11-20 | 苏州百源基因技术有限公司 | It is a kind of for detecting nucleotide sequence group, kit and the method for Fusarium solani |
-
2021
- 2021-02-24 CN CN202110206459.7A patent/CN113999926A/en not_active Withdrawn
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108841985A (en) * | 2018-06-29 | 2018-11-20 | 苏州百源基因技术有限公司 | It is a kind of for detecting nucleotide sequence group, kit and the method for Fusarium solani |
Non-Patent Citations (1)
| Title |
|---|
| 王瑜等: "腐皮镰刀菌SYBR Green实时荧光定量PCR快速检测方法的建立", 微生物学免疫学进展 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107937599B (en) | Molecular marker KB2 for identifying clubroot resistance of Chinese cabbages, primers and application | |
| CN105368935B (en) | SSR primer sets and method for the Purity Identification of capsicum variety Huifeng 2 | |
| CN116497147A (en) | DNA bar code and primer for screening high-quality Tibetan brown mushrooms and application of DNA bar code and primer | |
| KR101073000B1 (en) | Method of discriminating ginseng cultivars using STS primers | |
| Madadi et al. | Assessment of genetic variation within commercial Iranian pomegranate (Punica granatum L.) cultivars, using ISSR and SSR markers | |
| CN109628635B (en) | Development and application of gene marker for regulating purple color of capsicum olivum | |
| CN110734921A (en) | Detection method of kinds of anthracnose bacteria Colletotrichum siamense of tea trees | |
| CN106868138A (en) | Primer and kit for identifying the pathogen of tomato neckrot root rot and droop | |
| CN110804675B (en) | Microsatellite DNA marker fingerprint spectrum of non-heading Chinese cabbage and application thereof | |
| CN103114145B (en) | Soybean mosaic virus major gene locus and application thereof | |
| CN111850155A (en) | Application of specific target primer in simultaneous and rapid identification of two pathogenic bacteria of strawberry infection | |
| CN108977573B (en) | Method for identifying purity of seven-star radish hybrid by using SSR molecular marker | |
| CN108018375B (en) | Molecular marker KB1 for identifying clubroot resistance of Chinese cabbages, primers and application | |
| CN108165652B (en) | Specific molecular marker TGMI001 for identifying sex of torreya grandis at seedling stage | |
| CN104278028B (en) | It is positioned at haynaldia villosa 6VS DNA and penetrates into wheat anti-powdery mildew NIL sequence and application | |
| CN113999926A (en) | Primer group, kit and application of kit in rapid identification of xanthoxylum | |
| CN113637791B (en) | Molecular marker for simultaneously identifying restorability and authenticity of pepper male sterile three-line hybrid and identification method thereof | |
| CN112725521B (en) | Dendrobium chrysotoxum SSR molecular marker primer composition and application thereof | |
| CN104561338A (en) | Fingerprint spectrum of cauliflower hybrid and construction method thereof | |
| CN108411029A (en) | Balsam pear grows green No. 2 hybrid seed purities identification specific marker and method | |
| CN115109864A (en) | SSR molecular marker E201 for identifying Chinese pumpkin 'Zhongchuanu No. 1' hybrid, and primer, kit and method thereof | |
| CN106755541B (en) | Molecular marker, primer and probe for identifying lyophyllum decastes | |
| CN106868145B (en) | Molecular marker, primer and probe for identifying tricholoma plumbum | |
| KR101909786B1 (en) | Primer set for detecting Rosellinia necatrix and method for detecting using the same | |
| CN114634991B (en) | InDel marker for identifying high-variety coconuts and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20220201 |