CN113999803A - Deodorizing microbial agent and preparation method thereof - Google Patents
Deodorizing microbial agent and preparation method thereof Download PDFInfo
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- CN113999803A CN113999803A CN202111439539.3A CN202111439539A CN113999803A CN 113999803 A CN113999803 A CN 113999803A CN 202111439539 A CN202111439539 A CN 202111439539A CN 113999803 A CN113999803 A CN 113999803A
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 230000001877 deodorizing effect Effects 0.000 title abstract description 11
- 241000193755 Bacillus cereus Species 0.000 claims abstract description 15
- 241000194107 Bacillus megaterium Species 0.000 claims abstract description 15
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 15
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 15
- 241000194031 Enterococcus faecium Species 0.000 claims abstract description 15
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 15
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 15
- 230000001580 bacterial effect Effects 0.000 claims abstract description 15
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 15
- 239000000725 suspension Substances 0.000 claims abstract description 15
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 14
- 238000009630 liquid culture Methods 0.000 claims abstract description 14
- 241000605272 Acidithiobacillus thiooxidans Species 0.000 claims abstract description 13
- 239000002781 deodorant agent Substances 0.000 claims abstract description 10
- 238000002156 mixing Methods 0.000 claims abstract description 9
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 15
- 238000004332 deodorization Methods 0.000 claims description 14
- 241001052560 Thallis Species 0.000 claims description 13
- 239000001963 growth medium Substances 0.000 claims description 13
- 238000005119 centrifugation Methods 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 10
- 238000000855 fermentation Methods 0.000 claims description 5
- 230000004151 fermentation Effects 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims 3
- 238000011081 inoculation Methods 0.000 claims 1
- 239000002068 microbial inoculum Substances 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 16
- 239000010806 kitchen waste Substances 0.000 abstract description 5
- 230000003213 activating effect Effects 0.000 abstract description 3
- 244000052616 bacterial pathogen Species 0.000 abstract description 2
- 239000002253 acid Substances 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 12
- 239000000126 substance Substances 0.000 description 9
- 239000000243 solution Substances 0.000 description 6
- ROSDSFDQCJNGOL-UHFFFAOYSA-N Dimethylamine Chemical compound CNC ROSDSFDQCJNGOL-UHFFFAOYSA-N 0.000 description 4
- 239000002054 inoculum Substances 0.000 description 4
- 239000007789 gas Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 2
- LSDPWZHWYPCBBB-UHFFFAOYSA-N Methanethiol Chemical compound SC LSDPWZHWYPCBBB-UHFFFAOYSA-N 0.000 description 2
- 238000010170 biological method Methods 0.000 description 2
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 2
- 238000000053 physical method Methods 0.000 description 2
- 150000003568 thioethers Chemical class 0.000 description 2
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000873 masking effect Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 230000009967 tasteless effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002912 waste gas Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2251/00—Reactants
- B01D2251/95—Specific microorganisms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Environmental & Geological Engineering (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Disinfection, Sterilisation Or Deodorisation Of Air (AREA)
Abstract
The invention discloses a deodorizing microbial agent and a preparation method thereof, and is characterized in that the deodorizing microbial agent is prepared by activating 10-25 parts of thiobacillus thiooxidans, 25-40 parts of bacillus subtilis, 5-25 parts of bacillus megaterium, 10-25 parts of enterococcus faecium, 20-30 parts of lactobacillus plantarum and 10-25 parts of bacillus cereus, then carrying out liquid culture, preparing concentrated bacterial suspension by a centrifugal method, and mixing bacterial suspensions of various strains according to a certain proportion. The deodorant can effectively eliminate the acid odor and peculiar smell of the kitchen waste and kill various germs.
Description
Technical Field
The invention relates to the field of deodorant production, and particularly relates to a kitchen waste deodorant.
Background
Kitchen waste refers to residues of daily cooking and discarded food and the like, is rich in organic matters such as starch, cellulose, protein, lipid and the like, and can breed pathogenic bacteria and generate stink when being improperly treated, thereby seriously polluting the environment. The kitchen waste can generate a large amount of malodorous gases such as ammonia gas, hydrogen sulfide, thioether, dimethylamine, mercaptan and the like due to putrefaction in the treatment process.
At present, the deodorization method mainly comprises a physical method, a chemical method and a biological method. The physical method is to adjust the concentration of the malodorous substances in the atmosphere by adopting ways of masking, diluting, adsorbing and the like so as to achieve the aim of deodorization; the chemical method is that the malodorous substances are converted into tasteless substances through some chemical reactions; the physical deodorization and chemical deodorization have short retention time, compact equipment and high starting speed, and are suitable for large-flow waste gas and high-concentration gas pollution. However, the physical deodorization does not completely remove odor molecules by dilution and absorption, and the problem of secondary pollution in the chemical deodorization process is still not solved. The biological method adopts microbial degradation to convert malodorous substances into carbon dioxide, water, sulfate, nitrate and the like, chemical substances are not required to be added in the treatment process, the treatment can be carried out at normal temperature (10-40 ℃) and under normal pressure, secondary pollution is not caused, the operation is simpler, and the cost is lower.
The most common microbial deodorization methods at present mainly comprise a biological filter, a biological trickling filter, a biological washing tank and a microbial agent deodorization method. The microbial agent deodorization is to remove odor in the environment by utilizing the form of inoculating microbes, has the advantages of high deodorization efficiency, no secondary pollution and other biological deodorization methods, does not need to consider the problems of equipment investment and land occupation compared with biological filters and other modes, and is more convenient to use and lower in cost.
Aiming at the problem that a large amount of malodorous gases such as ammonia gas, hydrogen sulfide, thioether, dimethylamine, mercaptan and the like are generated due to the putrefaction effect of the kitchen waste in the treatment process, thiobacillus thiooxidans, bacillus subtilis, bacillus megaterium, enterococcus faecium, lactobacillus plantarum and bacillus cereus are selected, activated and then subjected to liquid expansion culture, then concentrated bacterial suspensions are prepared by a centrifugal method, and the bacterial suspensions of all the strains are mixed according to a certain proportion to prepare the deodorizing microbial agent.
Disclosure of Invention
The invention aims to overcome the defects of the prior art, and the deodorant microbial agent is prepared by selecting thiobacillus thiooxidans, bacillus subtilis, bacillus megaterium, enterococcus faecium, lactobacillus plantarum and bacillus cereus as deodorant strains, activating, then expanding liquid culture, then preparing concentrated bacterial suspension by a centrifugal method, and uniformly mixing bacterial suspensions of all strains according to a certain proportion. .
The technical scheme adopted by the invention for solving the technical problems is as follows:
the invention provides a deodorizing microbial agent, which is prepared by taking bacillus subtilis, bacillus megaterium, enterococcus faecium, lactobacillus plantarum and bacillus cereus as strains, activating, then carrying out liquid culture, then preparing concentrated bacterial suspension by a centrifugal method, and uniformly mixing bacterial suspensions of all the strains according to a certain proportion.
The preparation method of the deodorizing microbial agent comprises the following steps:
(1) the thiobacillus thiooxidans, the bacillus subtilis, the bacillus megaterium, the enterococcus faecium, the lactobacillus plantarum and the bacillus cereus are respectively put into test tubes filled with sterilized liquid culture media and cultured for 7 days in an incubator with the temperature of 35 ℃ and the relative humidity of 50 percent to obtain activated thalli.
(2) 100 ml of the culture solution was aspirated from the glycerol tube and inoculated into 4 ml of sterilized LB medium in a test tube, and cultured overnight in a shaker at 60 ℃. The activated strain was inoculated from a test tube LB medium at an inoculum size of 1% into a shake flask containing 100 ml of LB medium and cultured continuously in a shaker at 60 ℃ for 24 hours.
(3) And (4) centrifuging and collecting thalli after fermentation is finished, wherein the centrifugation condition is 6000 r/min, and the centrifugation is carried out for 20 min. The supernatant was discarded to obtain solid cells. Re-dissolving the centrifuged thallus of each strain in a sterilized liquid culture medium according to the mass ratio of 1:500, and uniformly mixing the suspension of each strain according to a certain volume ratio to obtain the liquid microbial deodorant.
The bacterial liquid in the step (3) is prepared from the following components in percentage by volume:
10-25 parts of thiobacillus thiooxidans, 25-40 parts of bacillus subtilis, 5-25 parts of bacillus megaterium, 10-25 parts of enterococcus faecium, 20-30 parts of lactobacillus plantarum and 10-25 parts of bacillus cereus.
The invention has the beneficial effects that: the deodorizing microbial preparation is prepared with Bacillus subtilis, Bacillus megaterium, enterococcus faecium, Lactobacillus plantarum and Bacillus cereus as bacteria, and through activation, liquid culture, centrifuging to prepare concentrated bacterial suspension, and mixing the bacterial suspensions in certain proportion.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the following describes technical solutions in the embodiments of the present invention clearly and completely in combination with the embodiments of the present invention. The described embodiments are only some, but not all embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
the deodorizing microbial agent and the preparation method thereof comprise the following steps:
(1) the thiobacillus thiooxidans, the bacillus subtilis, the bacillus megaterium, the enterococcus faecium, the lactobacillus plantarum and the bacillus cereus are respectively put into test tubes filled with sterilized liquid culture media and cultured for 7 days in an incubator with the temperature of 35 ℃ and the relative humidity of 50 percent to obtain activated thalli.
(2) 100 ml of the culture solution was aspirated from the glycerol tube and inoculated into 4 ml of sterilized LB medium in a test tube, and cultured overnight in a shaker at 60 ℃. The activated strain was inoculated from a test tube LB medium at an inoculum size of 1% into a shake flask containing 100 ml of LB medium and cultured continuously in a shaker at 60 ℃ for 24 hours.
(3) And (4) centrifuging and collecting thalli after fermentation is finished, wherein the centrifugation condition is 6000 r/min, and the centrifugation is carried out for 20 min. The supernatant was discarded to obtain solid cells. And (2) re-dissolving the thalli of each strain after centrifugation by using a corresponding sterilized liquid culture medium according to the mass ratio of 1:500, and uniformly mixing bacterial suspensions according to the volume fractions of 10 parts of thiobacillus thiooxidans, 25 parts of bacillus subtilis, 5 parts of bacillus megaterium, 15 parts of enterococcus faecium, 20 parts of lactobacillus plantarum and 25 parts of bacillus cereus to obtain the liquid microbial deodorant.
Example 2:
the deodorizing microbial agent and the preparation method thereof comprise the following steps:
(1) the thiobacillus thiooxidans, the bacillus subtilis, the bacillus megaterium, the enterococcus faecium, the lactobacillus plantarum and the bacillus cereus are respectively put into test tubes filled with sterilized liquid culture media and cultured for 7 days in an incubator with the temperature of 35 ℃ and the relative humidity of 50 percent to obtain activated thalli.
(2) 100 ml of the culture solution was aspirated from the glycerol tube and inoculated into 4 ml of sterilized LB medium in a test tube, and cultured overnight in a shaker at 60 ℃. The activated strain was inoculated from a test tube LB medium at an inoculum size of 1% into a shake flask containing 100 ml of LB medium and cultured continuously in a shaker at 60 ℃ for 24 hours.
(3) And (4) centrifuging and collecting thalli after fermentation is finished, wherein the centrifugation condition is 6000 r/min, and the centrifugation is carried out for 20 min. The supernatant was discarded to obtain solid cells. And (2) re-dissolving the thalli of each strain after centrifugation by using a corresponding sterilized liquid culture medium according to the mass ratio of 1:500, and uniformly mixing bacterial suspensions according to the volume fractions of 15 parts of thiobacillus thiooxidans, 30 parts of bacillus subtilis, 5 parts of bacillus megaterium, 10 parts of enterococcus faecium, 20 parts of lactobacillus plantarum and 20 parts of bacillus cereus to obtain the liquid microbial deodorant.
Example 3:
the deodorizing microbial agent and the preparation method thereof comprise the following steps:
(1) the thiobacillus thiooxidans, the bacillus subtilis, the bacillus megaterium, the enterococcus faecium, the lactobacillus plantarum and the bacillus cereus are respectively put into test tubes filled with sterilized liquid culture media and cultured for 7 days in an incubator with the temperature of 35 ℃ and the relative humidity of 50 percent to obtain activated thalli.
(2) 100 ml of the culture solution was aspirated from the glycerol tube and inoculated into 4 ml of sterilized LB medium in a test tube, and cultured overnight in a shaker at 60 ℃. The activated strain was inoculated from a test tube LB medium at an inoculum size of 1% into a shake flask containing 100 ml of LB medium and cultured continuously in a shaker at 60 ℃ for 24 hours.
(3) And (4) centrifuging and collecting thalli after fermentation is finished, wherein the centrifugation condition is 6000 r/min, and the centrifugation is carried out for 20 min. The supernatant was discarded to obtain solid cells. And (2) re-dissolving the thalli of each strain after centrifugation by using a corresponding sterilized liquid culture medium according to the mass ratio of 1:500, and uniformly mixing bacterial suspensions according to the volume fractions of 25 parts of thiobacillus thiooxidans, 25 parts of bacillus subtilis, 10 parts of bacillus megaterium, 10 parts of enterococcus faecium, 20 parts of lactobacillus plantarum and 10 parts of bacillus cereus to obtain the liquid microbial deodorant.
The applicant states that the present invention is illustrated in detail by the above examples, but the present invention is not limited to the above detailed methods, i.e. it is not meant that the present invention must rely on the above detailed methods for its implementation. It should be understood by those skilled in the art that any modification of the present invention, effective replacement of the raw materials of the product of the present invention, and selection of specific modes, etc., are within the scope and disclosure of the present invention.
Claims (2)
1. A deodorization microbial agent and a preparation method thereof are characterized in that the deodorization microbial agent and the preparation method thereof are as follows: (1) respectively putting thiobacillus thiooxidans, bacillus subtilis, bacillus megaterium, enterococcus faecium, lactobacillus plantarum and bacillus cereus in test tubes filled with sterilized liquid culture media, and culturing in an incubator with the temperature of 35 ℃ and the relative humidity of 50% for 7 days to obtain activated thalli; (2) taking 100 ml of bacterium liquid from a preserved glycerol tube, inoculating the bacterium liquid into 4 ml of sterilized test tube LB culture medium, and culturing in a shaking table at 60 ℃ overnight; inoculating the activated strain into a shake flask containing 100 ml of LB culture medium in a test tube LB culture medium with the inoculation amount of 1%, and continuously culturing for 24 hours in a shaking table at 60 ℃; (3) centrifuging and collecting thalli after fermentation is finished, wherein the centrifugation condition is 6000 r/min, and centrifuging for 20 min; discarding the supernatant to obtain solid thallus; re-dissolving the centrifuged thallus of each strain in a sterilized liquid culture medium according to the mass ratio of 1:500, and uniformly mixing the suspension of each strain according to a certain volume ratio to obtain the liquid microbial deodorant.
2. The microbial inoculum for deodorization and the preparation method thereof as claimed in claim 1, wherein each bacterial liquid in the step (3) comprises the following components by volume: 10-25 parts of thiobacillus thiooxidans, 25-40 parts of bacillus subtilis, 5-25 parts of bacillus megaterium, 10-25 parts of enterococcus faecium, 20-30 parts of lactobacillus plantarum and 10-25 parts of bacillus cereus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202111439539.3A CN113999803A (en) | 2021-11-30 | 2021-11-30 | Deodorizing microbial agent and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202111439539.3A CN113999803A (en) | 2021-11-30 | 2021-11-30 | Deodorizing microbial agent and preparation method thereof |
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| Publication Number | Publication Date |
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| CN113999803A true CN113999803A (en) | 2022-02-01 |
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| CN202111439539.3A Pending CN113999803A (en) | 2021-11-30 | 2021-11-30 | Deodorizing microbial agent and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114107115A (en) * | 2021-11-30 | 2022-03-01 | 电子科技大学中山学院 | Composite microbial inoculum for efficiently degrading kitchen waste and preparation method thereof |
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2021
- 2021-11-30 CN CN202111439539.3A patent/CN113999803A/en active Pending
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| Title |
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| 白瑞;胡阳;雷振宇;王晓雪;杨茗;黄瑶瑶;: "复合微生物制剂在环保领域中的应用", 应用化工 * |
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| CN114107115A (en) * | 2021-11-30 | 2022-03-01 | 电子科技大学中山学院 | Composite microbial inoculum for efficiently degrading kitchen waste and preparation method thereof |
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