CN113984734B - Background subtraction detection method and system for Raman spectrum and Raman spectrometer - Google Patents
Background subtraction detection method and system for Raman spectrum and Raman spectrometer Download PDFInfo
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Abstract
The invention relates to a background subtraction detection method of Raman spectrum,Under the preset condition, the system and the Raman spectrometer sequentially collect the first time delay delta t after each time of pulse laser emission according to the time sequence 1 To Deltat 1 Raman scattered light of +w period and simultaneously collecting a second time delay Δt after each pulse of laser light 2 To Deltat 2 The quasi-synchronous detection of the Raman spectrum of the sample to be detected and the background spectrum of the detection background can be realized by the background light of the detection background in the +w period, the background spectrum of the detection background is subtracted from the Raman spectrum of the sample to be detected, the final Raman spectrum of the sample to be detected is obtained, error subtraction can be avoided, and the acquisition and subtraction of the Raman spectrum of the sample to be detected and the background spectrum of the detection background in a complex and rapidly-changing light environment can be satisfied, so that the final Raman spectrum of the sample to be detected is obtained, and the real Raman spectrum of the sample to be detected is obtained.
Description
Technical Field
The invention relates to the technical field of Raman spectrum detection, in particular to a background subtraction detection method and system of Raman spectrum and a Raman spectrometer.
Background
The signal of the Raman scattered light is weak and is 10 of Rayleigh scattering -6 ～10 -9 Magnitude. When raman spectrum acquisition is performed on a target substance by using a raman spectrometer, a very sensitive detector is required to acquire a weak signal, and the raman spectrum including the target substance also includes interference signals in various detection backgrounds, such as fluorescent backgrounds, ambient light backgrounds, detector noise, rayleigh scattering of laser, and the like, and the intensity of part of the interference signals is very strong. The ambient light background and detector noise are usually eliminated by strictly shielding the detection sampling device and the sample from light and turning off the laserOne or more sets of background signals are acquired under the same detection conditions and subtracted from the object signal. Since the raman signal is weak, the time for this signal and background noise acquisition varies from a few seconds to tens of seconds.
For the application of Raman spectrum with good light shielding condition and weak and stable background light, the method is effective, the background signal collected in the same time period can be approximately equal to the background signal collected in the Raman signal collecting process, but for the application that part of the Raman spectrum can not shield non-Raman scattering background light by a physical method and the background light change frequency is extremely fast, such as the state of a Raman spectrum in-situ online test catalyst in a photo-thermal catalytic reaction, and the combustion Raman spectrum detects the strong and extremely unstable light background environment generated in the experimental process; for example, remote Raman spectrum detection, optical path length detection, and complicated and uncontrollable ambient light background. At this time, if the conventional background collection and subtraction method is used, the light background environment has changed greatly within a period of several seconds or even tens of seconds, and the interference of the background light cannot be removed by the deduction method, that is, in the conventional background subtraction method, due to the long single collection time and long time interval, the error subtraction is caused for the fast-changing and unstable light background.
Disclosure of Invention
The invention aims to solve the technical problem of providing a background subtraction detection method and system for Raman spectrum and a Raman spectrometer aiming at the defects of the prior art.
The technical scheme of the background subtraction detection method of the Raman spectrum is as follows:
under the preset condition, sequentially collecting the first time delay delta t after each pulse laser is emitted according to the time sequence 1 To Deltat 1 Generating Raman spectrum of the sample to be detected by Raman scattered light in +w period, and simultaneously collecting second time delay delta t after each pulse laser is emitted 2 To Deltat 2 Generating a background spectrum of a detection background in a +w period, wherein w is the pulse width of pulse laser emitted by a laser in a Raman spectrometer;
and subtracting the background spectrum of the detection background from the Raman spectrum of the sample to be detected to obtain the final Raman spectrum of the sample to be detected.
The background subtraction detection method of the Raman spectrum has the following beneficial effects:
under the preset condition, sequentially collecting the first time delay delta t after each pulse laser is emitted according to the time sequence 1 To Deltat 1 Raman scattered light of +w period and simultaneously collecting a second time delay Δt after each pulse of laser light 2 To Deltat 2 The quasi-synchronous detection of the Raman spectrum of the sample to be detected and the background spectrum of the detection background can be realized by the background light of the detection background in the +w period, the background spectrum of the detection background is subtracted from the Raman spectrum of the sample to be detected, the final Raman spectrum of the sample to be detected is obtained, error subtraction can be avoided, and the acquisition and subtraction of the Raman spectrum of the sample to be detected and the background spectrum of the detection background in a complex and rapidly-changing light environment can be satisfied, so that the final Raman spectrum of the sample to be detected is obtained, and the real Raman spectrum of the sample to be detected is obtained.
Based on the scheme, the background subtraction detection method of the Raman spectrum can be improved as follows.
Further, the acquiring process of the first time delay includes:
calculating a first time delay Deltat according to a first formula 1 The first formula is:wherein L is the distance between the sampling probe of the Raman spectrometer and the sample to be measured, and c is the light speed.
Further, the second time-delayed acquisition process includes:
calculating a second time delay Deltat according to a second formula 2 The second formula is: Δt (delta t) 2 ＝Δt 1 +w+delta, wherein delta is a preset time length, and the value range of delta is 0-w.
The technical scheme of the background subtraction detection system of the Raman spectrum is as follows:
the acquisition generation module is used for:
under the preset condition, sequentially collecting the first time delay delta t after each pulse laser is emitted according to the time sequence 1 To Deltat 1 Generating Raman spectrum of the sample to be detected by Raman scattered light in +w period, and simultaneously collecting second time delay delta t after each pulse laser is emitted 2 To Deltat 2 Generating a background spectrum of a detection background in a +w period, wherein w is the pulse width of pulse laser emitted by a laser in a Raman spectrometer;
the deduction module is used for: and subtracting the background spectrum of the detection background from the Raman spectrum of the sample to be detected to obtain the final Raman spectrum of the sample to be detected.
The background subtraction detection system of the Raman spectrum has the following beneficial effects:
under the preset condition, sequentially collecting the first time delay delta t after each pulse laser is emitted according to the time sequence 1 To Deltat 1 Raman scattered light of +w period and simultaneously collecting a second time delay Δt after each pulse of laser light 2 To Deltat 2 The quasi-synchronous detection of the Raman spectrum of the sample to be detected and the background spectrum of the detection background can be realized by the background light of the detection background in the +w period, the background spectrum of the detection background is subtracted from the Raman spectrum of the sample to be detected, the final Raman spectrum of the sample to be detected is obtained, error subtraction can be avoided, and the acquisition and subtraction of the Raman spectrum of the sample to be detected and the background spectrum of the detection background in a complex and rapidly-changing light environment can be satisfied, so that the final Raman spectrum of the sample to be detected is obtained, and the real Raman spectrum of the sample to be detected is obtained.
Based on the above scheme, the background subtraction detection system of the Raman spectrum can be improved as follows.
Further, the device also comprises an acquisition module, wherein the acquisition module is used for:
calculating a first time delay Deltat according to a first formula 1 The first formulaThe method comprises the following steps:wherein L is the distance between the sampling probe of the Raman spectrometer and the sample to be measured, and c is the light speed.
Further, the acquisition module is further configured to:
calculating a second time delay Deltat according to a second formula 2 The second formula is: Δt (delta t) 2 ＝Δt 1 +w+delta, wherein delta is a preset time length, and the value range of delta is 0-w.
The technical scheme of the Raman spectrometer is as follows:
comprising a control chip for performing a background subtraction detection method of raman spectroscopy as described in any one of the preceding claims.
Drawings
FIG. 1 is a flow chart of a background subtraction detection method of Raman spectrum according to an embodiment of the invention;
FIG. 2 is a schematic structural diagram of a Raman spectrometer;
FIG. 3 is a graph showing the timing of laser pulses, the acquisition timing of the photo detector Raman signal, and the acquisition timing of the photo detector background signal;
FIG. 4 Raman spectrum of sample to be measured;
FIG. 5 is a background spectrum of detection background;
FIG. 6 is a graph showing the Raman spectrum of a sample to be measured and the average spectrum accumulated over multiple measurements of the detection background;
FIG. 7 is a final Raman spectrum of a sample to be measured;
FIG. 8 is a schematic diagram of a background subtraction detection system of Raman spectrum according to an embodiment of the present invention;
Detailed Description
As shown in fig. 1, a background subtraction detection method of raman spectrum according to an embodiment of the present invention includes the following steps:
s1, under the preset condition, sequentially collecting a first time delay delta t after each pulse laser is emitted according to a time sequence 1 To Deltat 1 Of +w periodRaman scattered light is generated to generate a raman spectrum of the sample to be measured, and a second time delay deltat after each pulse laser emission is simultaneously acquired 2 To Deltat 2 Generating a background spectrum of a detection background in a +w period, wherein w is the pulse width of pulse laser emitted by a laser in a Raman spectrometer;
s2, subtracting the background spectrum of the detection background from the Raman spectrum of the sample to be detected to obtain the final Raman spectrum of the sample to be detected.
Wherein, S1 and S2 are specifically realized by a raman spectrometer, as shown in fig. 2, the raman spectrometer includes: the device comprises a laser 1, a Raman spectrum probe, namely a sampling probe 2, a spectrum analyzer 4 and a photoelectric detector 3, wherein the laser 1 can be a pulse laser 1, such as a picosecond pulse laser, a nanosecond pulse laser, a microsecond pulse laser and the like; the photodetector 3 can be a time-gating photodetector 3, and specifically, the photodetector 3 with the same time-gating precision as a pulse laser is selected for detecting a raman spectrum signal and a background signal in synchronization with the pulse laser in a time domain, and can be a photomultiplier, an ICCD with an image intensifier, an ICMOS, and the like. The specific detection principle is as follows:
the laser 1 is used for sending pulse laser for exciting the sample 5 to be detected to the sample 5 to be detected, the sample 5 to be detected generates Raman scattered light under the excitation of the pulse laser, the photoelectric detector 3 collects the Raman scattered light of the sample 5 to be detected and sends the Raman scattered light to the spectrum analyzer 4, and the spectrum analyzer 4 is used for decomposing the complex-color Raman scattered light into monochromatic spectrum signals and analyzing the monochromatic spectrum signals, so that the Raman spectrum of the sample 5 to be detected is obtained. And the raman detection process is known to those skilled in the art, and will not be described herein.
A first time delay delta t after each pulse laser is sequentially acquired 1 To Deltat 1 Collecting a second time delay Deltat after each pulse laser emission while the Raman scattered light of +w period 2 To Deltat 2 Background light of detection background of +w period, wherein detection background refers to: the first time delay deltat after each time of emitting pulse laser is collected in turn 1 To Deltat 1 The environment background where the raman scattered light in +w period is located detects that the background light exists in the background, and the photodetector 3 also receives the background light in the detection environment to generate a background spectrum of the detection background.
The process of obtaining the raman spectrum of the sample 5 to be measured in S1 is explained as follows: for example, the laser is pulsed at a frequency of 10 shots per second, a pulse width w=10ns, a first time delay Δt 1 =100 ns; then:
at time 0, the pulse laser is emitted for the first time, and a first time delay delta t is obtained after the pulse laser is emitted for the first time 1 To Deltat 1 Raman scattered light S in +w period, i.e. 100ns to 110ns period 1 ；
At 0.1 second, the pulse laser is emitted for the second time, and the first time delay deltat after the pulse laser is emitted for the second time is collected 1 To Deltat 1 Raman scattered light S for +w period, i.e. 0.1s+100ns to 0.1s+110ns period 2 ；
At 0.2 seconds, the pulse laser is emitted for the third time, and the first time delay deltat after the pulse laser is emitted for the third time is collected 1 To Deltat 1 Raman scattered light S for +w period, i.e. 0.2s+100ns to 0.2s+110ns period 3 ；
By analogy, the first time delay deltat after each pulse laser emission is collected 1 To Deltat 1 And obtaining Raman spectrum of the sample 5 to be detected by Raman scattered light in +w period, wherein the preset conditions are as follows: a preset duration, a first preset frequency threshold for collecting Raman scattered light, or a second preset frequency threshold for emitting pulse laser;
in actual operation, the gate width W is the laser light pulse width of the laser 1, typically on the order of ps to ns; initializing target raman signal intensity s=0, and detecting accumulated times i=1; the spectrum analyzer 43 collects a single-pulse excitation raman spectrum signal S with a gate width W having a time delay Δt by emitting a single-pulse laser with a pulse width W i When the number of times of collecting the Raman scattered light reaches a first preset time threshold S th And ending the collection of the Raman signal of the sample 5 to be detected, otherwise, continuing to emit laser pulses and collecting. Thereby obtainingThe raman spectrum to the sample 5 to be measured is: s0=Σs i ；
The procedure for obtaining the background spectrum of the detection background in S2 is explained as follows:
for example, the laser is pulsed at a frequency of 10 shots per second, a pulse width w=10ns, a first time delay Δt 1 =100 ns, Δ=w=10ns, then Δt 2 ＝120ns：
At time 0, the pulse laser is emitted for the first time, and a second time delay delta t is obtained after the pulse laser is emitted for the first time 2 To Deltat 2 Background light of detection background in +w period, i.e. 120ns to 130ns period;
at 0.1 second, the pulse laser is emitted for the second time, and the first time delay deltat after the pulse laser is emitted for the second time is collected 2 To Deltat 2 Background light of detection background in +w period, i.e., 0.1s+120ns to 0.1s+130ns period;
at 0.2 seconds, the pulse laser is emitted for the third time, and the first time delay deltat after the pulse laser is emitted for the third time is collected 2 To Deltat 2 Background light of detection background in +w period, i.e. 0.2s+120ns to 0.2s+130ns period;
and so on, a second time delay delta t after each pulse laser emission is acquired 2 To Deltat 2 And generating a background spectrum of the detection background in +w time period.
The acquiring process of the first time delay includes:
calculating a first time delay Deltat according to a first formula 1 The first formula is:wherein L is the distance between the sampling probe 2 of the Raman spectrometer and the sample 5 to be measured, and c is the light velocity.
Wherein, the collection frequency f of the Raman scattered light of the sample 5 to be detected and the collection time window width w are consistent with the excitation light.
Wherein the second time-delayed acquisition process includes:
according to the second maleCalculating a second time delay Deltat 2 The second formula is: Δt (delta t) 2 ＝Δt 1 +w+delta, wherein w is the pulse width of the pulse laser emitted by the laser 1 in the Raman spectrometer, delta is the preset duration, and the value range of delta is 0-w.
Wherein, the collection frequency f of the Raman scattered light of the collection detection background and the collection time window width w are consistent with the excitation light.
Setting the total collection time as t, collecting N=t×f times of Raman scattered light of the sample 5 to be detected, and collecting N=t×f times of Raman scattered light of the detection background altogether, accumulating the N times of Raman scattered light of the sample 5 to be detected to obtain a Raman spectrum S0 of the sample 5 to be detected,S i the ith Raman scattered light of the sample 5 to be detected is represented, N times of background light of the detection background are accumulated, and a detection background spectrum B0,/is obtained>B i And (3) removing the detection background spectrum by using the formula Sr=S0-B0 to obtain the final Raman spectrum Sr of the sample 5 to be detected.
The timing sequence of the laser pulse, the acquisition timing sequence of the raman signal of the photodetector 3 and the acquisition timing sequence of the background signal of the photodetector 3 are shown in fig. 3, specifically:
1) 1 is the time sequence of pulse laser, the pulse width of the pulse laser is w, and the frequency is f;
2) 2 is the acquisition time sequence of the Raman signal of the photoelectric detector 3, the acquisition gate width is w, the frequency is f, and the delay relative to the laser pulse is deltat 0;
3) And 3 is the acquisition time sequence of the background signal of the photoelectric detector 3, the acquisition gate width is w, the frequency is f, and the delay relative to the acquisition time sequence of the Raman signal is w+delta.
The following describes the technical effects of a background subtraction detection method of raman spectrum applied by the present application by using a remote laser raman spectrometer to detect the sample 5 at a distance of 100 meters:
detection distance: l=100 m;
pulse width of laser 1: w=10ns;
excitation light frequency: f=5 Hz;
buffer time, Δ=w=10ns;
detection time: t=3s;
first time delay: Δt (delta t) 1 ＝667ns；
Second time delay: Δt (delta t) 2 ＝687ns；
Optical background: and (3) adding an illumination LED light source in the natural light background, wherein the flicker frequency is 2 times of the AC frequency of the commercial power, namely 100Hz.
In the collection time of 3s, the remote laser Raman spectrometer collects 15 groups of Raman scattered lights of the sample 5 to be detected and 15 groups of detection background lights, and due to the tiny fluctuation of natural background lights in 3s and the stroboscopic fluctuation of the LED lamps, certain difference exists in the background of the collected signals, the time span of the Raman scattered lights and the detection background lights of the same group of sample 5 to be detected is only 30ns, the light background can be considered to be relatively constant in such a short time, and the deduction of the Raman spectrum can be performed to subtract the light background noise in a quasi-real time.
The raman spectrum of the sample 5 to be measured is shown in fig. 4, the detected background spectrum is shown in fig. 5, the average curve of the raman spectrum of the sample 5 to be measured and the detected background spectrum is shown in fig. 6, and the final raman spectrum of the sample 5 to be measured is shown in fig. 7.
As can be seen from fig. 4 and 5, there is a certain fluctuation in the background of the light acquired by multiple acquisitions, resulting in the same fluctuation in the acquired background spectrum; as can be seen from fig. 3 and 4, the light background subtracted by the present invention has a good effect, and the influence of the fluctuation of the background can be completely eliminated.
According to the background subtraction detection method of the Raman spectrum, under the preset condition, the first time delay delta t after each pulse laser is emitted is sequentially collected according to the time sequence 1 To Deltat 1 Raman scattered light of +w period and simultaneously collecting a second time delay after each pulse of laser lightΔt 2 To Deltat 2 The quasi-synchronous detection of the Raman spectrum and the detection background spectrum of the sample 5 to be detected can be realized by the background light of the detection background in the +w period, the detection background spectrum is subtracted from the Raman spectrum of the sample 5 to be detected, the final Raman spectrum of the sample 5 to be detected is obtained, error subtraction can be avoided, and the acquisition and subtraction of the Raman spectrum and the detection background spectrum of the sample 5 to be detected in a complex and rapidly-changing light environment can be satisfied, so that the final Raman spectrum of the sample 5 to be detected is obtained, and the real Raman spectrum of the sample 5 to be detected is obtained.
In the above embodiments, although steps S1, S2, etc. are numbered, only specific embodiments are given herein, and those skilled in the art may adjust the execution sequence of S1, S2, etc. according to the actual situation, which is also within the scope of the present invention, and it is understood that some embodiments may include some or all of the above embodiments.
As shown in fig. 8, a background subtraction detection system 200 of raman spectrum according to an embodiment of the present invention includes an acquisition generation module 210 and a subtraction module 220;
the acquisition generation module 210 is configured to:
under the preset condition, sequentially collecting the first time delay delta t after each pulse laser is emitted according to the time sequence 1 To Deltat 1 Generating Raman spectrum of the sample to be detected by Raman scattered light in +w period, and simultaneously collecting second time delay delta t after each pulse laser is emitted 2 To Deltat 2 Generating a background spectrum of a detection background in a +w period, wherein w is the pulse width of pulse laser emitted by a laser in a Raman spectrometer;
the deduction module 220 is configured to: and subtracting the Raman spectrum of the detection background from the Raman spectrum of the sample 5 to be detected to obtain the final Raman spectrum of the sample 5 to be detected.
Under the preset condition, sequentially collecting the first time delay delta t after each pulse laser is emitted according to the time sequence 1 To Deltat 1 Raman scattered light of +w period and simultaneously collecting a second time after each pulse of laser lightDelay deltat 2 To Deltat 2 The quasi-synchronous detection of the Raman spectrum of the sample 5 to be detected and the Raman spectrum of the detection background can be realized by the background light of the detection background in +w time period, the Raman spectrum of the detection background is subtracted from the Raman spectrum of the sample 5 to be detected, the final Raman spectrum of the sample 5 to be detected is obtained, erroneous subtraction can be avoided, and the acquisition and subtraction of the Raman spectrum of the sample 5 to be detected and the Raman spectrum of the detection background in a complex and rapidly-changing light environment can be satisfied, so that the final Raman spectrum of the sample 5 to be detected is obtained, and the real Raman spectrum of the sample 5 to be detected is obtained.
Preferably, in the above technical solution, the device further includes an acquisition module, where the acquisition module is configured to:
calculating a first time delay Deltat according to a first formula 1 The first formula is:wherein L is the distance between the sampling probe 2 of the Raman spectrometer and the sample 5 to be measured, and c is the light velocity.
Preferably, in the above technical solution, the obtaining module is further configured to:
calculating a second time delay Deltat according to a second formula 2 The second formula is: Δt (delta t) 2 ＝Δt 1 +w+delta, wherein delta is a preset time length, and the value range of delta is 0-w.
The steps for implementing the corresponding functions of the parameters and the unit modules in the background subtraction detection system 200 for raman spectrum according to the present invention can refer to the parameters and the steps in the embodiments of the background subtraction detection method for raman spectrum according to the present invention, and are not described herein. The method can also be improved and upgraded in the existing Raman spectrometer, and a program corresponding to the quantitative detection method based on Raman spectrum is embedded in the method.
The raman spectrometer comprises a control chip, wherein the control chip is used for the background subtraction detection method of the raman spectrum.
Those skilled in the art will appreciate that the present invention may be implemented as a system, method, or computer program product.
Accordingly, the present disclosure may be embodied in the following forms, namely: either entirely hardware, entirely software (including firmware, resident software, micro-code, etc.), or entirely software, or a combination of hardware and software, referred to herein generally as a "circuit," module "or" system. Furthermore, in some embodiments, the invention may also be embodied in the form of a computer program product in one or more computer-readable media, which contain computer-readable program code.
Any combination of one or more computer readable media may be employed. The computer readable medium may be a computer readable signal medium or a computer readable storage medium. The computer readable storage medium can be, for example, an electronic, magnetic, optical, electromagnetic, infrared, or semiconductor system, apparatus, or device, or any combination thereof. More specific examples (a non-exhaustive list) of the computer-readable storage medium include an electrical connection having one or more wires, a portable computer diskette, a hard disk, a Random Access Memory (RAM), a read-only memory (ROM), an erasable programmable read-only memory (EPROM or flash memory), an optical fiber, a portable compact disc read-only memory (CD-ROM), an optical storage device, a magnetic storage device, or any suitable combination thereof. In this document, a computer readable storage medium may be any tangible medium that can contain, or store a program for use by or in connection with an instruction execution system, apparatus, or device.
While embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are illustrative and not to be construed as limiting the invention, and that variations, modifications, alternatives and variations may be made to the above embodiments by one of ordinary skill in the art within the scope of the invention.
Claims (3)
1. A method of background subtraction detection of raman spectra, comprising:
at a preset barUnder the part, sequentially collecting the first time delay delta t after each time of emitting pulse laser according to time sequence 1 To Deltat 1 Generating Raman spectrum of the sample to be detected by Raman scattered light in +w period, and simultaneously collecting second time delay delta t after each pulse laser is emitted 2 To Deltat 2 Generating a background spectrum of a detection background in a +w period, wherein w is the pulse width of pulse laser emitted by a laser in a Raman spectrometer;
subtracting the background spectrum of the detection background from the Raman spectrum of the sample to be detected to obtain the final Raman spectrum of the sample to be detected;
the first time delay acquisition process includes:
calculating the first time delay Deltat according to a first formula 1 The first formula is:wherein L is the distance between a sampling probe of the Raman spectrometer and the sample to be detected, and c is the light speed;
the second time delay acquisition process includes:
calculating the second time delay deltat according to a second formula 2 The second formula is: Δt (delta t) 2 ＝Δt 1 +w+delta, wherein delta is a preset time length, and the value range of delta is 0-w.
2. The background subtraction detection system of the Raman spectrum is characterized by comprising an acquisition generation module and a subtraction module;
the acquisition generation module is used for:
under the preset condition, sequentially collecting the first time delay delta t after each pulse laser is emitted according to the time sequence 1 To Deltat 1 Generating Raman spectrum of the sample to be detected by Raman scattered light in +w period, and simultaneously collecting second time delay delta t after each pulse laser is emitted 2 To Deltat 2 Generating a background spectrum of a detection background in a +w period, wherein w is the background light of the detection backgroundA laser in the Raman spectrometer emits pulse width of pulse laser;
the deduction module is used for: subtracting the background spectrum of the detection background from the Raman spectrum of the sample to be detected to obtain the final Raman spectrum of the sample to be detected;
the device further comprises an acquisition module, wherein the acquisition module is used for:
calculating the first time delay Deltat according to a first formula 1 The first formula is:wherein L is the distance between a sampling probe of the Raman spectrometer and the sample to be detected, and c is the light speed;
the acquisition module is further configured to:
calculating the second time delay deltat according to a second formula 2 The second formula is: Δt (delta t) 2 ＝Δt 1 +w+delta, wherein delta is a preset time length, and the value range of delta is 0-w.
3. A raman spectrometer comprising a control chip configured to perform a method of background subtraction detection of raman spectra as recited in claim 1.
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| CN105866099A (en) * | 2016-05-16 | 2016-08-17 | 天津大学 | Raman spectrum acquisition system with low-fluorescence background |
| CN106596499A (en) * | 2016-11-01 | 2017-04-26 | 北京华泰诺安技术有限公司 | Real-time Raman spectrum calibration method |
| GB2572662A (en) * | 2018-10-05 | 2019-10-09 | Res & Innovation Uk | Raman spectrometer |
| CN209707379U (en) * | 2019-02-21 | 2019-11-29 | 中智科仪（北京）科技有限公司 | Portable remote Raman spectrum system based on Gao Zhongying nanosecoud pulse laser |
| CN113092437A (en) * | 2021-03-25 | 2021-07-09 | 东莞市中科原子精密制造科技有限公司 | Remote Raman spectrum detection module and remote detection spectrometer |
| CN113109317A (en) * | 2021-03-26 | 2021-07-13 | 中国科学院西安光学精密机械研究所 | Raman spectrum quantitative analysis method and system based on background subtraction extraction peak area |
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2021
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Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105866099A (en) * | 2016-05-16 | 2016-08-17 | 天津大学 | Raman spectrum acquisition system with low-fluorescence background |
| CN106596499A (en) * | 2016-11-01 | 2017-04-26 | 北京华泰诺安技术有限公司 | Real-time Raman spectrum calibration method |
| GB2572662A (en) * | 2018-10-05 | 2019-10-09 | Res & Innovation Uk | Raman spectrometer |
| CN209707379U (en) * | 2019-02-21 | 2019-11-29 | 中智科仪（北京）科技有限公司 | Portable remote Raman spectrum system based on Gao Zhongying nanosecoud pulse laser |
| CN113092437A (en) * | 2021-03-25 | 2021-07-09 | 东莞市中科原子精密制造科技有限公司 | Remote Raman spectrum detection module and remote detection spectrometer |
| CN113109317A (en) * | 2021-03-26 | 2021-07-13 | 中国科学院西安光学精密机械研究所 | Raman spectrum quantitative analysis method and system based on background subtraction extraction peak area |
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