CN113980806B - Vessel device for cell culture growth - Google Patents
Vessel device for cell culture growth Download PDFInfo
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- CN113980806B CN113980806B CN202111254743.8A CN202111254743A CN113980806B CN 113980806 B CN113980806 B CN 113980806B CN 202111254743 A CN202111254743 A CN 202111254743A CN 113980806 B CN113980806 B CN 113980806B
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- clamping plate
- placing groove
- culture bag
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/14—Bags
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
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- Biotechnology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
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- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a vessel device for cell culture growth, which belongs to the technical field of cell culture and is used for accommodating a cell culture bag of cells and a shaking mechanism for shaking the cells in the cell culture bag uniformly, wherein the shaking mechanism comprises a protective shell, a placing groove which is slidably arranged in the protective shell and is used for accommodating the cell culture bag, a swing arm mechanism which is arranged on the protective shell and drives the protective shell to swing reciprocally, and a clamping mechanism which is detachably arranged in the placing groove and is used for fixing the cell culture bag. The invention can culture different kinds of cells by regulating and controlling the using area of the cell culture bag, the oscillation angle and the fluctuation speed of the oscillating mechanism, and is also suitable for mass industrialized production.
Description
Technical Field
The invention relates to the technical field of cell culture, in particular to a vessel device for cell culture growth.
Background
Cell culture is also known as cell cloning technology, a formal biological term being cell culture technology. Cell culture is an essential process for the whole biotechnology, or for one of the biological cloning techniques, and is itself a large-scale cloning of cells. The cell culture technique can be that one cell is subjected to mass culture to become simple single cells or little differentiated multicellular, and a large number of cells or metabolites thereof are finally obtained, and in the process of cell culture, a vessel device for cell culture growth is a necessary device.
Most of the existing culture devices for culturing cells can only culture cells under static state, and when the cultured cells are added in a vessel, the distribution of the cells in the culture device is difficult to be uniform, so that the culture number of the cells is limited, and the normal development of individual cells is influenced.
Disclosure of Invention
1. Technical problem to be solved
The present invention is directed to a vessel device for cell culture growth, which solves the above-mentioned problems of the prior art.
2. Technical proposal
In order to solve the problems, the invention adopts the following technical scheme:
a household utensils device for cell culture growth, including the cell culture bag that is arranged in holding the cell and shake even vibration mechanism to the cell in the cell culture bag, vibration mechanism includes the protective housing, slidable mounting just is arranged in the standing groove that holds the cell culture bag inside the protective housing, installs on the protective housing and drives the reciprocal wobbling swing arm mechanism of protective housing, can dismantle and locate the inside fixture that just fixes the cell culture bag of standing groove.
Further, the top end opening of the protective shell is provided, and the opening is hinged with a protective cover.
Further, the clamping mechanisms are provided with two groups, the clamping mechanisms can be used for adjusting the area of the cell culture bag for cell culture, the two sides of the cell culture bag along the length direction of the cell culture bag are provided with edge abdication areas which are in one-to-one correspondence with the clamping mechanisms and are used for containing the corresponding clamping mechanisms, and when the two clamping mechanisms are positioned on the corresponding edge abdication areas, blank areas on the inner side of the cell culture bag are all used for cell culture.
Still further, fixture includes the splint first that is used for hugging closely cell culture bag bottom face and is used for hugging closely cell culture bag up end second, the length of splint first is greater than the length of splint second, and the both ends of splint second pass through locking mechanism first and splint first detachable connection, the both ends of splint first pass through locking mechanism second and are connected with the interior bottom of standing groove.
Furthermore, the first locking mechanism comprises a first screw rod fixedly arranged at two sides of the top end of the first clamping plate, and a first nut sleeved on the first screw rod and fixing the second clamping plate on the first clamping plate, and the second clamping plate is provided with a second through hole which corresponds to the first screw rod one by one and is used for the first screw rod to vertically pass through.
Furthermore, the second locking mechanism comprises a second screw rod which is arranged at the inner bottom of the placing groove in a sliding manner and is of a T-shaped structure, a second nut which is sleeved on the second screw rod and fixes the first clamping plate at the inner bottom of the placing groove, a first through hole which corresponds to the second screw rod one by one and is used for the second screw rod to vertically pass through is formed in the first clamping plate, and two T-shaped sliding grooves which are used for the T-shaped blocks at the bottom end of the second screw rod to slide are formed in the inner bottom of the placing groove.
Further, soft adhesive tapes are respectively adhered to the opposite end surfaces of the first clamping plate and the second clamping plate.
Further, the swing arm mechanism comprises a swing rod, a driving unit and a cam, wherein the swing rod is arranged on the side wall of the length direction of the placing groove and is close to the first connecting shaft at one side of the end part of the swing arm mechanism, the swing rod is rotatably sleeved on the first connecting shaft, the driving unit is arranged on the side wall of the protective shell, the cam is arranged on the output end of the driving unit through a coupler and is positioned on the inner side of the protective shell, and the cam is rotatably connected with the free end of the swing rod through the second connecting shaft.
Still further, the bottom middle part of standing groove is equipped with the connecting block, the inside of protective housing has the connecting seat through slide mechanism sliding connection, and the connecting seat passes through the articulated shaft and rotates with the connecting block to be connected, slide mechanism is including locating the T type slider in standing groove bottom middle part, set up in the protective housing bottom and supply the gliding slide of T type slider.
3. Advantageous effects
1. The invention adjusts the area used by the cell culture bag through the sliding of the two groups of clamping mechanisms on the cell culture bag, namely the area between the two groups of clamping mechanisms corresponds to the culture area of the cell culture bag.
2. According to the invention, the clamping plate II can be fixed on the clamping plate I through the threaded connection of the nut I on the screw rod I, so that the cell culture bag can be fixed between the clamping plate II and the clamping plate I, in addition, the clamping plate I can be fixed at the inner bottom of the placing groove through the threaded connection of the nut II and the screw rod II, and as the screw rod II is provided with four screws, when the nut II is screwed on the corresponding screw rod II, four different parts of the cell culture bag can be fixed in the placing groove, so that the stability of the cell culture bag when the cell culture bag is oscillated is ensured, in addition, the distance between the two screw rods II in the same T-shaped chute is adjusted through the sliding of the bottom end of the screw rod II in the T-shaped chute, so that the two screw rods II in the same T-shaped chute can be smoothly inserted in the through holes I on the clamping plates of two different distances, and the flexibility of the placing groove for clamping the cell culture bag is improved.
3. According to the invention, the front swing arm unit swings under the action of the driving unit, the placing groove can swing back and forth under the cooperation of the rear swing arm unit, and the cell culture bag has a vibration effect on cultured cells in the cell culture bag, so that the cells can be uniformly positioned in the cell culture bag and can be contacted with cell culture liquid, normal growth of the cells is ensured, and in addition, the cells in the cell culture bag are vibrated.
4. According to the invention, through controlling the rotation angle and the rotation speed of the driving unit, the placing groove is repeatedly swung in the protective shell at a certain frequency under the mutual cooperation of the swing arm mechanism and the sliding mechanism, so that the oscillating mechanism can oscillate different types of cells at a certain fluctuation speed, and the oscillating mechanism can be suitable for culturing different types of cells.
5. According to the invention, the two ends of the swinging rod are respectively connected with the placing groove and the cam in a rotating way, the bottom end of the placing groove is hinged with the connecting seat, the bottom end of the connecting seat is in sliding connection with the inner bottom of the protective shell, the front end and the rear end of the placing groove swing along an elliptical track, and when the rear end of the placing groove swings along the elliptical track downwards and upwards, the front end of the placing groove swings along the elliptical track upwards and downwards, and the front end and the rear end of the placing groove are respectively provided with an oscillation similar to the elliptical track, so that the oscillating effect of the device on cells in the cell culture bag is greatly improved, the cells can be uniformly positioned in the cell culture bag, and can be uniformly contacted with a cell culture solution, thereby being beneficial to cell culture.
Drawings
FIG. 1 is a schematic diagram of the front internal structure of the present invention;
FIG. 2 is a schematic diagram of the connection of the placement tank and the swing arm mechanism;
FIG. 3 is a schematic top view of the connection of the placement tank to the cell culture bag;
FIG. 4 is a schematic diagram of the structure of a cell culture bag.
Reference numerals: 1. a protective housing; 2. a placement groove; 3. a protective cover; 4. a cell culture bag; 41. an edge yield area; 5. a clamping plate I; 51. a first through hole; 6. a clamping plate II; 61. a second through hole; 7. a soft adhesive tape; 8. a first screw; 9. a first nut; 10. a second screw; 11. a second nut; 12. a T-shaped chute; 13. a first connecting shaft; 14. a swinging rod; 15. a second connecting shaft; 16. a cam; 17. a connecting block; 18. a connecting seat; 19. a T-shaped slider; 20. a slideway; 21. and a driving unit.
Detailed Description
The invention will now be described in further detail with reference to the drawings and examples.
Examples
The vessel device for cell culture growth shown in fig. 1 comprises a cell culture bag 4 for containing cells and an oscillating mechanism for oscillating the cells in the cell culture bag 4, wherein a plurality of openings are formed in the same side surface of the cell culture bag 4, a pipeline for communicating external equipment with the cell culture bag 4 is arranged on each opening, namely, a pipeline is used for conveying nutrient substances for cell development, a pipeline is used for conveying gas generated in the cell development process, the oscillating mechanism comprises a protective shell 1, a placing groove 2 slidably mounted in the protective shell 1 and used for containing the cells culture bag 4, a swing arm mechanism mounted on the protective shell 1 and used for driving the protective shell 1 to oscillate reciprocally, a clamping mechanism which is detachably arranged in the placing groove 2 and used for fixing the cell culture bag 4, the top end opening of the protective shell 1 is provided, and a protective cover 3 is hinged at the opening;
as shown in fig. 1 and 4, two groups of clamping mechanisms are provided, the clamping mechanisms can also be used for adjusting the area of the cell culture bag 4 for cell culture, two sides of the cell culture bag 4 along the length direction of the cell culture bag are provided with edge abdication areas 41 which are in one-to-one correspondence with the clamping mechanisms and are used for accommodating the corresponding clamping mechanisms, when the two clamping mechanisms are positioned on the corresponding edge abdication areas 41, blank areas on the inner side of the cell culture bag 4 are all used for cell culture, the area used by the cell culture bag 4 is adjusted by sliding the two groups of clamping mechanisms on the cell culture bag 4, namely, the area between the two groups of clamping mechanisms corresponds to the culture area of the cell culture bag 4, the setting for adjusting the use area of the cell culture bag 4 can enable people to correspondingly adjust the culture area of the cell culture bag 4 according to the culture quantity of cells and the growth environment required by the cells, so as to ensure that cells can smoothly grow and be applicable to cell culture of different scales, the clamping mechanism comprises a clamping plate I5 used for clinging to the bottom end face of the cell culture bag 4 and a clamping plate II 6 used for clinging to the upper end face of the cell culture bag 4, the length of the clamping plate I5 is larger than that of the clamping plate II 6, two ends of the clamping plate II 6 are detachably connected with the clamping plate I5 through a locking mechanism I, two ends of the clamping plate I5 are connected with the inner bottom of the placing groove 2 through a locking mechanism II, the cell culture bag 4 can be fixed between the two clamping plates through butt joint connection between the clamping plate I5 and the clamping plate II 6, the cell culture bag 4 can be divided into three areas taking the two butt joint clamping plates as dividing lines through extrusion of the two butt joint clamping plates on the same part of the cell culture bag 4, and the area between the two groups of the clamping mechanisms is the area which can be used for containing cells, under the action of the two groups of clamping mechanisms, the cell culture solution and the cultured cells can be ensured not to leak outside into other two areas;
as shown in fig. 1, the opposite end surfaces of the two clamping plates 1 and 6 are respectively stuck with a soft adhesive tape 7, when the clamping plate 5 is in butt joint connection with the clamping plate 6, the extrusion effect of the clamping plate on the cell culture bag 4 can be improved through the deformation of the soft adhesive tape 7, and the sealing performance of the cell culture area on the cell culture bag 4 is ensured again;
as shown in fig. 1, the first locking mechanism comprises a first screw rod 8 fixedly arranged on two sides of the top end of the first clamping plate 5, and a first nut 9 sleeved on the first screw rod 8 and used for fixing the second clamping plate 6 on the first clamping plate 5, wherein the second clamping plate 6 is provided with a second through hole 61 which corresponds to the first screw rod 8 one by one and is used for the first screw rod 8 to vertically pass through, the first clamping plate 6 can be fixed on the first clamping plate 5 through the threaded connection of the first nut 9 on the first screw rod 8, and meanwhile, the cell culture bag 4 is fixed between the second clamping plate 6 and the first clamping plate 5.
As shown in fig. 1, 2 and 3, the locking mechanism II comprises a screw rod II 10 which is slidably arranged at the inner bottom of the placing groove 2 and is in a T-shaped structure, and a nut II 11 which is sleeved on the screw rod II 10 and fixes the clamping plate I5 at the inner bottom of the placing groove 2, wherein the clamping plate I5 is provided with a through hole I51 which corresponds to the screw rod II 10 one by one and is used for the screw rod II 10 to vertically pass through, the inner bottom of the placing groove 2 is provided with two T-shaped sliding grooves 12 for the T-shaped blocks at the bottom of the screw rod II 10 to slide, the clamping plate I5 can be fixed at the inner bottom of the placing groove 2 through the threaded connection of the nut II 11 and the screw rod II 10, and as the screw rod II 10 is provided with four nuts, when the nut II 11 is screwed on the corresponding screw rod II 10, four different parts of the cell culture bags 4 can be fixed in the placing groove 2 so as to ensure the stability of the cell culture bags 4 when being oscillated, and the distance between the two screw rods II 10 in the same T-shaped sliding grooves 12 can be adjusted through the sliding of the bottom of the screw rod II 10 in the T-shaped sliding grooves 12, so that the two clamping plates in the same T-shaped sliding grooves 12 can be smoothly inserted into the two clamping grooves 4 at the same distance between the two clamping plates 5 in the same flexible distance between the two clamping plates and the screw grooves 2;
as shown in fig. 1 and 2, the swing arm mechanism comprises a first connecting shaft 13 installed on the side wall of the length direction of the placing groove 2 and close to one side of the end part of the placing groove 2, a swing rod 14 rotationally sleeved on the first connecting shaft 13, a driving unit 21 installed on the side wall of the protective shell 1, and a cam 16 installed on the output end of the driving unit 21 through a coupler and positioned on the inner side of the protective shell 1, wherein the cam 16 is rotationally connected with the free end of the swing rod 14 through a second connecting shaft 15, a connecting block 17 is arranged in the middle of the bottom end of the placing groove 2, a connecting seat 18 is slidingly connected with the inside of the protective shell 1 through a sliding mechanism, the connecting seat 18 is rotationally connected with the connecting block 17 through a hinged shaft, the sliding mechanism comprises a T-shaped sliding block 19 arranged in the middle of the bottom end of the placing groove 2, a sliding way 20 arranged at the inner bottom of the protective shell 1 and used for sliding the T-shaped sliding block 19, the driving unit 21 may be a motor, when the cam 16 rotates under the driving of the driving unit 21, the swinging rod 14 connected with the cam 16 and the placing groove 2 simultaneously rotates to swing, and because the bottom end of the placing groove 2 is hinged with the connecting seat 18, and the connecting seat 18 is slidably connected with the inner bottom of the protecting shell 1, the placing groove 2 is driven to move back and forth in the horizontal direction in the protecting shell 1 when the swinging rod 14 swings, and the swinging rod swings up and down around the hinge shaft on the connecting seat 18 when moving back and forth horizontally, so that the placing groove 2 can sufficiently oscillate the cultured cells in the cell culture bag 4, the cells can be ensured to be uniformly positioned in the cell culture bag 4, and the cells can be contacted with the cell culture solution.
The specific application process of the vessel device for cell culture growth comprises the following steps: when the cell culture bag is used, firstly, the protective cover 3 is opened, the second nut 11 is unscrewed from the second screw rod 10, the first clamping plate 5 and the second clamping plate 6 arranged on the first clamping plate are removed, secondly, the first nut 9 is rotated to adjust the distance between the second clamping plate 6 and the first clamping plate 5, one end of the cell culture bag 4 which is not filled with nutrient solution passes through a gap between the second clamping plate 6 and the first clamping plate 5, secondly, the other end of the cell culture bag 4 passes through the gap between the second clamping plate 6 and the first clamping plate 5 through the same method, secondly, the distance between the two clamping plates is adjusted according to the quantity of cell culture, namely, the adjustment of the using area of the cell culture bag 4 is realized, secondly, the first clamping plate 6 is fixed on the first clamping plate 5 through the threaded connection of the first nut 9, meanwhile, the cell culture bag 4 is fixed between the second clamping plate 6 and the first clamping plate 5, when the first clamping plate 5 and the second clamping plate 6 are in butt joint connection, the extrusion effect of the clamping plate on the cell culture bag 4 can be improved through deformation of the soft adhesive tape 7, and the sealing performance of a cell culture area on the cell culture bag 4 is ensured again;
secondly, the bottom end of the screw rod II 10 is manually driven to slide in the T-shaped sliding groove 12, the distance between the two screw rods II 10 in the same T-shaped sliding groove 12 is adjusted, so that the two screw rods II 10 in the same T-shaped sliding groove 12 can be smoothly inserted into the through holes I51 on the clamping plates I5 with different distances, the clamping plates I5 are fixed at the inner bottom of the placing groove 2 through the threaded connection of the nuts II 11 and the screw rods II 10, and because the screws II 10 are provided with four, when the nuts II 11 are screwed on the corresponding screws II 10, the four different parts of the cell culture bag 4 can be fixed in the placing groove 2, so that the stability of the cell culture bag 4 when being oscillated is ensured;
secondly, the driving unit 21 is started to enable the swing arm mechanism to swing, the cam 16 rotates under the driving of the driving unit 21, and the swing rod 14 connected with the cam 16 and the placing groove 2 rotates to swing, so that a pulling force or a pushing force is applied to one end, connected with the swing rod 14, of the placing groove 2, the placing groove 2 can slide back and forth in the protective shell 1 through the pushing force or the pulling force, so that the front end and the rear end of the placing groove 2 swing in an elliptical track similar to the inside of the protective shell 1 through the sliding connection of the placing groove 2 and the protective shell 1 and the swinging interaction of the swing rod 14, and when the rear end of the placing groove 2 swings upwards downwards and upwards on the elliptical track, the front end of the placing groove 2 swings upwards and downwards again on the elliptical track, and the front end and the rear end of the placing groove 2 swing similar to the elliptical track respectively, so that the oscillating effect of the device on the cell culture bag 4 can be greatly improved, the cell can be uniformly located in the cell culture bag 4, and can be uniformly contacted with a cell culture vessel, and the cell culture vessel can be conveniently grown in the process.
It will be appreciated by persons skilled in the art that the above embodiments are provided for illustration only and not for limitation of the invention, and that variations and modifications of the above described embodiments will fall within the scope of the claims of the invention as long as they fall within the true spirit of the invention.
Claims (5)
1. The vessel device for cell culture growth is characterized by comprising a cell culture bag (4) for containing cells and an oscillating mechanism for shaking the cells in the cell culture bag (4), wherein the oscillating mechanism comprises a protective shell (1), a placing groove (2) which is slidably arranged in the protective shell (1) and is used for containing the cell culture bag (4), a swing arm mechanism which is arranged on the protective shell (1) and drives the protective shell (1) to swing back and forth, and a clamping mechanism which is detachably arranged in the placing groove (2) and is used for fixing the cell culture bag (4);
the swing arm mechanism comprises a first connecting shaft (13) which is arranged on the side wall of the length direction of the placing groove (2) and is close to one side of the end part of the placing groove, a swing rod (14) which is rotatably sleeved on the first connecting shaft (13), a driving unit (21) which is arranged on the side wall of the protective shell (1), and a cam (16) which is arranged on the output end of the driving unit (21) through a coupler and is positioned on the inner side of the protective shell (1), wherein the cam (16) is rotatably connected with the free end of the swing rod (14) through a second connecting shaft (15);
the middle part of the bottom end of the placing groove (2) is provided with a connecting block (17), the inside of the protective shell (1) is slidably connected with a connecting seat (18) through a sliding mechanism, the connecting seat (18) is rotationally connected with the connecting block (17) through a hinge shaft, and the sliding mechanism comprises a T-shaped sliding block (19) arranged in the middle part of the bottom end of the placing groove (2) and a sliding way (20) which is arranged at the inner bottom of the protective shell (1) and is used for the sliding of the T-shaped sliding block (19);
the clamping mechanism comprises a clamping plate I (5) which is used for being clung to the bottom end face of the cell culture bag (4) and a clamping plate II (6) which is used for being clung to the upper end face of the cell culture bag (4), the length of the clamping plate I (5) is larger than that of the clamping plate II (6), two ends of the clamping plate II (6) are detachably connected with the clamping plate I (5) through a locking mechanism I, and two ends of the clamping plate I (5) are connected with the inner bottom of the placing groove (2) through a locking mechanism II;
the first locking mechanism comprises a first screw rod (8) fixedly arranged on two sides of the top end of the first clamping plate (5), and a first nut (9) sleeved on the first screw rod (8) and used for fixing the second clamping plate (6) on the first clamping plate (5), wherein the second clamping plate (6) is provided with a second through hole (61) which corresponds to the first screw rod (8) one by one and is used for the first screw rod (8) to vertically pass through.
2. Vessel device for cell culture growth according to claim 1, characterized in that the top end opening of the protective housing (1) is provided with a protective cover (3) hinged at the opening.
3. A vessel device for cell culture growth according to claim 1, wherein two groups of clamping mechanisms are provided, and the clamping mechanisms can be used for adjusting the area of the cell culture bag (4) for cell culture, the two sides of the cell culture bag (4) along the length direction of the cell culture bag are provided with edge relief areas (41) which are in one-to-one correspondence with the clamping mechanisms and are used for accommodating the corresponding clamping mechanisms, and when the two clamping mechanisms are positioned on the corresponding edge relief areas (41), blank areas on the inner side of the cell culture bag (4) are all used for cell culture.
4. The vessel device for cell culture growth according to claim 3, wherein the second locking mechanism comprises a second screw rod (10) which is slidably arranged at the inner bottom of the placing groove (2) and is of a T-shaped structure, and a second nut (11) which is sleeved on the second screw rod (10) and fixes the first clamping plate (5) at the inner bottom of the placing groove (2), the first clamping plate (5) is provided with a first through hole (51) which corresponds to the second screw rod (10) one by one and is used for the second screw rod (10) to vertically pass through, and the inner bottom of the placing groove (2) is provided with two T-shaped sliding grooves (12) which are used for the T-shaped blocks at the bottom end of the second screw rod (10) to slide.
5. The vessel device for cell culture growth according to claim 4, wherein soft adhesive strips (7) are adhered to the opposite end surfaces of the first clamping plate (5) and the second clamping plate (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202111254743.8A CN113980806B (en) | 2021-10-27 | 2021-10-27 | Vessel device for cell culture growth |
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| CN202111254743.8A CN113980806B (en) | 2021-10-27 | 2021-10-27 | Vessel device for cell culture growth |
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| CN113980806A CN113980806A (en) | 2022-01-28 |
| CN113980806B true CN113980806B (en) | 2023-08-15 |
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Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101668843A (en) * | 2007-04-27 | 2010-03-10 | 东洋制罐株式会社 | Cell culture apparatus, cell culture system and cell culture method |
| JP2010136628A (en) * | 2008-12-09 | 2010-06-24 | Ihi Corp | Cell culture bag and cell culture method thereof |
| CN201990682U (en) * | 2010-08-25 | 2011-09-28 | 北京多赢时代科技有限公司 | Swinging type cell culture device |
| JP2012239401A (en) * | 2011-05-17 | 2012-12-10 | Toyo Seikan Kaisha Ltd | Cell-culturing method and cell-culturing system |
| CN105567565A (en) * | 2016-03-08 | 2016-05-11 | 上海吉凯基因科技有限公司 | Continuous cell culture rocking device |
| CN212800390U (en) * | 2020-04-04 | 2021-03-26 | 苏州生动细胞生物科技有限公司 | Variable volume cell culture bag |
| TWM610899U (en) * | 2020-12-17 | 2021-04-21 | 基亞生物科技股份有限公司 | Automatically cell-cultivating device |
| WO2021114088A1 (en) * | 2019-12-10 | 2021-06-17 | 苏州生动细胞生物科技有限公司 | Variable-volume cell culture bag |
| CN213570422U (en) * | 2020-09-28 | 2021-06-29 | 湖南省肿瘤医院 | Immune cell culture bag regularly rocks device |
| CN214032527U (en) * | 2020-11-09 | 2021-08-24 | 河南茵特赛尔生物技术有限公司 | Cell culture support |
| CN214270915U (en) * | 2020-12-17 | 2021-09-24 | 成都慧晟生物技术有限公司 | With supporting shaking device that uses of immune cell culture bag |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012141055A1 (en) * | 2011-04-13 | 2012-10-18 | 株式会社日立製作所 | Cell culture device and transport device |
-
2021
- 2021-10-27 CN CN202111254743.8A patent/CN113980806B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101668843A (en) * | 2007-04-27 | 2010-03-10 | 东洋制罐株式会社 | Cell culture apparatus, cell culture system and cell culture method |
| JP2010136628A (en) * | 2008-12-09 | 2010-06-24 | Ihi Corp | Cell culture bag and cell culture method thereof |
| CN201990682U (en) * | 2010-08-25 | 2011-09-28 | 北京多赢时代科技有限公司 | Swinging type cell culture device |
| JP2012239401A (en) * | 2011-05-17 | 2012-12-10 | Toyo Seikan Kaisha Ltd | Cell-culturing method and cell-culturing system |
| CN105567565A (en) * | 2016-03-08 | 2016-05-11 | 上海吉凯基因科技有限公司 | Continuous cell culture rocking device |
| WO2021114088A1 (en) * | 2019-12-10 | 2021-06-17 | 苏州生动细胞生物科技有限公司 | Variable-volume cell culture bag |
| CN212800390U (en) * | 2020-04-04 | 2021-03-26 | 苏州生动细胞生物科技有限公司 | Variable volume cell culture bag |
| CN213570422U (en) * | 2020-09-28 | 2021-06-29 | 湖南省肿瘤医院 | Immune cell culture bag regularly rocks device |
| CN214032527U (en) * | 2020-11-09 | 2021-08-24 | 河南茵特赛尔生物技术有限公司 | Cell culture support |
| TWM610899U (en) * | 2020-12-17 | 2021-04-21 | 基亞生物科技股份有限公司 | Automatically cell-cultivating device |
| CN214270915U (en) * | 2020-12-17 | 2021-09-24 | 成都慧晟生物技术有限公司 | With supporting shaking device that uses of immune cell culture bag |
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| CN113980806A (en) | 2022-01-28 |
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