CN113980797B - 一种用于核酸扩增物的检测装置及检测方法 - Google Patents
一种用于核酸扩增物的检测装置及检测方法 Download PDFInfo
- Publication number
- CN113980797B CN113980797B CN202111277311.9A CN202111277311A CN113980797B CN 113980797 B CN113980797 B CN 113980797B CN 202111277311 A CN202111277311 A CN 202111277311A CN 113980797 B CN113980797 B CN 113980797B
- Authority
- CN
- China
- Prior art keywords
- detection
- cavity
- nucleic acid
- amplification
- opening
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 226
- 230000003321 amplification Effects 0.000 title claims abstract description 110
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 110
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 74
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 74
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 74
- 238000010790 dilution Methods 0.000 claims abstract description 67
- 239000012895 dilution Substances 0.000 claims abstract description 67
- 238000007789 sealing Methods 0.000 claims abstract description 55
- 238000012360 testing method Methods 0.000 claims abstract description 43
- 238000006243 chemical reaction Methods 0.000 claims abstract description 18
- 238000007865 diluting Methods 0.000 claims abstract description 18
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 14
- 238000000034 method Methods 0.000 claims abstract description 11
- 239000000872 buffer Substances 0.000 claims description 23
- 239000012528 membrane Substances 0.000 claims description 13
- 238000004891 communication Methods 0.000 claims description 11
- 230000001225 therapeutic effect Effects 0.000 claims 2
- 230000009471 action Effects 0.000 abstract description 10
- 230000008901 benefit Effects 0.000 abstract description 6
- 230000008569 process Effects 0.000 abstract description 6
- 238000005516 engineering process Methods 0.000 description 8
- 238000010586 diagram Methods 0.000 description 7
- 238000003745 diagnosis Methods 0.000 description 6
- 239000007853 buffer solution Substances 0.000 description 5
- 238000012123 point-of-care testing Methods 0.000 description 5
- 239000007788 liquid Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 239000003344 environmental pollutant Substances 0.000 description 3
- 238000011010 flushing procedure Methods 0.000 description 3
- 238000011901 isothermal amplification Methods 0.000 description 3
- 231100000719 pollutant Toxicity 0.000 description 3
- 238000007397 LAMP assay Methods 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000711573 Coronaviridae Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 102000018120 Recombinases Human genes 0.000 description 1
- 108010091086 Recombinases Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000001823 molecular biology technique Methods 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000005382 thermal cycling Methods 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
Claims (6)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202111277311.9A CN113980797B (zh) | 2021-10-29 | 2021-10-29 | 一种用于核酸扩增物的检测装置及检测方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202111277311.9A CN113980797B (zh) | 2021-10-29 | 2021-10-29 | 一种用于核酸扩增物的检测装置及检测方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN113980797A CN113980797A (zh) | 2022-01-28 |
CN113980797B true CN113980797B (zh) | 2024-05-24 |
Family
ID=79744938
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202111277311.9A Active CN113980797B (zh) | 2021-10-29 | 2021-10-29 | 一种用于核酸扩增物的检测装置及检测方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN113980797B (zh) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115505515B (zh) * | 2022-09-28 | 2024-01-23 | 广纳达康(广州)生物科技有限公司 | 一种二段式核酸检测装置及检测方法 |
CN115466671B (zh) * | 2022-09-28 | 2023-12-15 | 广纳达康(广州)生物科技有限公司 | 一种防冲液核酸扩增物检测装置及检测方法 |
CN115678751B (zh) * | 2022-12-30 | 2023-04-18 | 北京金诺美科技股份有限公司 | 一种样品管 |
Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH07204252A (ja) * | 1994-01-14 | 1995-08-08 | Material Eng Tech Lab Inc | 輸液容器 |
CN105154314A (zh) * | 2015-09-22 | 2015-12-16 | 广州和实生物技术有限公司 | 一种集提取、扩增和检测一体化的基因检测装置 |
CN111548900A (zh) * | 2019-02-12 | 2020-08-18 | 浙江大学 | 一种用于核酸扩增及检测的装置和检测方法 |
CN212199219U (zh) * | 2020-04-23 | 2020-12-22 | 湖北省妇幼保健院 | 一种可自带试剂的核酸扩增管 |
CN212357257U (zh) * | 2020-06-19 | 2021-01-15 | 安徽为臻生物工程技术有限公司 | 一种用于核酸扩增物快速检测的防污染检测装置 |
CN212833778U (zh) * | 2019-12-31 | 2021-03-30 | 上海星耀医学科技发展有限公司 | 一种解脲支原体核酸快速检测试剂 |
CN212955133U (zh) * | 2020-07-22 | 2021-04-13 | 杭州奥盛仪器有限公司 | 一种两步法核酸扩增、检测用的反应管及反应管套装 |
CN213447134U (zh) * | 2020-06-24 | 2021-06-15 | 固安北吉生物科技有限公司 | 一种密闭核酸扩增及检测一体化装置 |
CN113150965A (zh) * | 2021-04-21 | 2021-07-23 | 西安交通大学 | 一种防污染检测管和基于其的crispr分子诊断检测方法 |
CN113388489A (zh) * | 2021-06-29 | 2021-09-14 | 广东粤港澳大湾区国家纳米科技创新研究院 | 防污染单腔体核酸检测装置 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003033740A2 (en) * | 2001-07-10 | 2003-04-24 | Massachusetts Institute Of Technology | Apparatus and method for isolating a nucleic acid |
JP2022083347A (ja) * | 2020-11-24 | 2022-06-03 | タクセル株式会社 | 検査機器 |
-
2021
- 2021-10-29 CN CN202111277311.9A patent/CN113980797B/zh active Active
Patent Citations (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH07204252A (ja) * | 1994-01-14 | 1995-08-08 | Material Eng Tech Lab Inc | 輸液容器 |
CN105154314A (zh) * | 2015-09-22 | 2015-12-16 | 广州和实生物技术有限公司 | 一种集提取、扩增和检测一体化的基因检测装置 |
CN111548900A (zh) * | 2019-02-12 | 2020-08-18 | 浙江大学 | 一种用于核酸扩增及检测的装置和检测方法 |
CN212833778U (zh) * | 2019-12-31 | 2021-03-30 | 上海星耀医学科技发展有限公司 | 一种解脲支原体核酸快速检测试剂 |
CN212199219U (zh) * | 2020-04-23 | 2020-12-22 | 湖北省妇幼保健院 | 一种可自带试剂的核酸扩增管 |
CN212357257U (zh) * | 2020-06-19 | 2021-01-15 | 安徽为臻生物工程技术有限公司 | 一种用于核酸扩增物快速检测的防污染检测装置 |
CN213447134U (zh) * | 2020-06-24 | 2021-06-15 | 固安北吉生物科技有限公司 | 一种密闭核酸扩增及检测一体化装置 |
CN212955133U (zh) * | 2020-07-22 | 2021-04-13 | 杭州奥盛仪器有限公司 | 一种两步法核酸扩增、检测用的反应管及反应管套装 |
CN113150965A (zh) * | 2021-04-21 | 2021-07-23 | 西安交通大学 | 一种防污染检测管和基于其的crispr分子诊断检测方法 |
CN113388489A (zh) * | 2021-06-29 | 2021-09-14 | 广东粤港澳大湾区国家纳米科技创新研究院 | 防污染单腔体核酸检测装置 |
Non-Patent Citations (1)
Title |
---|
不同核酸提取试剂盒在新型冠状病毒核酸检测中的比较研究;马雯;张伟宏;马瑛龙;葸静;金哲宇;朴文花;;分子诊断与治疗杂志(第05期);第829-833页 * |
Also Published As
Publication number | Publication date |
---|---|
CN113980797A (zh) | 2022-01-28 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN113980797B (zh) | 一种用于核酸扩增物的检测装置及检测方法 | |
US11974846B2 (en) | Biological fluid transfer device and biological fluid sampling system | |
JP6339663B2 (ja) | 生体液採集装置、および、生体液採集検査システム | |
US10668473B2 (en) | Reagent preparation assembly | |
JP6158426B2 (ja) | 生体液サンプリング移送装置並びに生体液分離及び検査システム | |
RU2509533C2 (ru) | Устройство для отбора и дозирования образцов | |
CN113846004B (zh) | 一种旋转式核酸扩增物检测装置及检测方法 | |
CN105836240B (zh) | 破管机构、包含有该破管机构的密封性试管组件及其应用 | |
US10342471B2 (en) | Biological fluid transfer device and biological fluid sampling system | |
CN216192304U (zh) | 一种用于核酸扩增物的检测装置 | |
US9186670B2 (en) | Functionalized microfluidic device and method | |
WO2023138148A1 (zh) | 一种应用于快速检测的密封装置及其使用方法、应用 | |
CN104293659A (zh) | 一种全封闭一体式核酸扩增与检测管 | |
CN204536025U (zh) | 一种一次性样本收集瓶 | |
CN107102000B (zh) | 一种核酸检测装置 | |
CN205574438U (zh) | 破管机构及包含有该破管机构的密封性试管组件 | |
CN112903365A (zh) | 粪便取样检测一体化装置和粪便检测盒 | |
WO2018068377A1 (zh) | 一种液体样本导流装置及含有该导流装置的检测设备 | |
CN218404216U (zh) | 一种核酸检测连接件及核酸扩增检测组件 | |
CN113960027B (zh) | 一种即时检测的笔状装置 | |
KR102458777B1 (ko) | 생물학적 유체 수집 및 안정화 시스템 | |
CN216303834U (zh) | 一种用于核酸扩增物的检测装置 | |
CN216192303U (zh) | 一种核酸扩增检测装置 | |
CN214270826U (zh) | 一种反应装置 | |
CN219122048U (zh) | 一种自测采样检测装置 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20230412 Address after: 510000 Room 201, building D, 136 Kaiyuan Avenue, Huangpu District, Guangzhou City, Guangdong Province Applicant after: Guangdong Guangdong Guangdong Hong Kong Macao Dawan District National Nanotechnology Innovation Research Institute Address before: 510000 Room 201, building D, 136 Kaiyuan Avenue, Huangpu District, Guangzhou City, Guangdong Province Applicant before: Guangdong Guangdong Guangdong Hong Kong Macao Dawan District National Nanotechnology Innovation Research Institute Applicant before: Guangna Dakang (Guangzhou) Biotechnology Co.,Ltd. |
|
TA01 | Transfer of patent application right | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20230705 Address after: Room 1-5 / F, building B4, Wuhan National Biological Industry (Jiufeng innovation) base, 666 Gaoxin Avenue, Donghu New Technology Development Zone, Wuhan City, Hubei Province, 430000 Applicant after: WUHAN NANO DIAGNOSIS FOR HEALTH BIOTECHNOLOGY CO.,LTD. Address before: 510000 Room 201, building D, 136 Kaiyuan Avenue, Huangpu District, Guangzhou City, Guangdong Province Applicant before: Guangdong Guangdong Guangdong Hong Kong Macao Dawan District National Nanotechnology Innovation Research Institute |
|
TA01 | Transfer of patent application right | ||
EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20220128 Assignee: Guangna Dakang (Guangzhou) Biotechnology Co.,Ltd. Assignor: WUHAN NANO DIAGNOSIS FOR HEALTH BIOTECHNOLOGY CO.,LTD. Contract record no.: X2023980045265 Denomination of invention: A detection device and method for nucleic acid amplification products License type: Exclusive License Record date: 20231101 |
|
EE01 | Entry into force of recordation of patent licensing contract | ||
GR01 | Patent grant | ||
GR01 | Patent grant |