CN113956101A - Soil improvement microbial agent and preparation method thereof - Google Patents

Soil improvement microbial agent and preparation method thereof Download PDF

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Publication number
CN113956101A
CN113956101A CN202111461218.3A CN202111461218A CN113956101A CN 113956101 A CN113956101 A CN 113956101A CN 202111461218 A CN202111461218 A CN 202111461218A CN 113956101 A CN113956101 A CN 113956101A
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soil
aqueous solution
mixed
component
microbial
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王薇
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Nanjing Wo You Biology Fertilizer Co ltd
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Nanjing Wo You Biology Fertilizer Co ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01BSOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
    • A01B79/00Methods for working soil
    • A01B79/02Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00Other inorganic fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00Soil-conditioning materials or soil-stabilising materials
    • C09K17/40Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2101/00Agricultural use
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2109/00MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE pH regulation

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Soil Sciences (AREA)
  • Environmental Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Life Sciences & Earth Sciences (AREA)
  • Materials Engineering (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Mechanical Engineering (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to the technical field of fruit tree soil improvement, and provides a soil improvement microbial agent which comprises two components, wherein the first component comprises: grass carbon, diatomite, chitosan, ganoderma lucidum polysaccharide and a mixed microbial agent, which comprise a mixed aqueous solution of oxoacid, methyl jasmonic acid and methyl jasmonate, and the preparation method thereof is provided, so that the plant health is remarkably promoted, the fruit setting and maturing rate is high, the color is uniform, the surface gloss is good, the color is bright, the fruits are large and uniform, the sugar degree is high, the yield is high, the soil granular structure is increased, the soil fertility is improved, the soil ecological environment is improved, the acidity of the soil is reduced, the soil hardening is prevented, the permeability is improved, and the fertility is accumulated, maintained and the water is preserved.

Description

Soil improvement microbial agent and preparation method thereof
Technical Field
The invention relates to the technical field of fruit tree soil improvement, in particular to a soil improvement microbial agent and a preparation method thereof.
Background
In the development process of human society, especially in recent times, the long-term application of chemical fertilizers brings serious negative problems of soil structure destruction, water body pollution, fertilizer utilization rate reduction, agricultural product quality reduction and the like. Soil is the most important production resource for agricultural planting, other substances cannot be replaced, and the regulation of soil, improvement of soil and restoration of soil activity are necessary ways for sustainable development.
The soil improvement methods commonly used include physical improvement measures, chemical improvement measures, engineering improvement measures and biological improvement measures. The microbial soil conditioner is an effective biological improvement measure, and can effectively improve the soil structure, increase the microbial biomass in the soil and change the soil activity. However, the quality and effect of the existing soil improvement agents are different, and in addition, although the fertility and activity of the soil are recovered to a certain degree, the conversion efficiency of crops planted in the soil to nutrients in the soil cannot be improved.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention provides the soil improvement microbial agent and the preparation method thereof, which can improve the soil hardening and restore the soil activity and simultaneously improve the conversion efficiency of crops to nutrient substances.
In order to achieve the above object, the technical solution of the first aspect of the present invention is:
a soil improvement microbial inoculant comprising the following components:
the component one: the composite material comprises the following raw materials in parts by weight: 5-10 parts of turf, 2-8 parts of diatomite, 1-5 parts of chitosan, 1-4 parts of ganoderma lucidum polysaccharide and 1 part of microbial agent, wherein the microbial agent is a microbial agent formed by independently fermenting microbial bacteria in a mixture, and the weight ratio of fermentation stock solutions of potassium bacteria, bacillus subtilis, lactobacillus acidophilus, bifidobacterium, actinomycetes, helicobacter and salmonella in the microbial agent is 1:0.5-3:0.5-3:0.5-3: 0.5-2: 0.5-3:0.5-2, wherein the effective amount of the microorganism in the microorganism agent is 1.2-1.4 hundred million/g;
and (2) component two: mixed water solution of oxy-water acid, methyl jasmonic acid and methyl jasmonate, wherein the concentration of salicylic acid is 20-400 mu m/l, the concentration of methyl jasmonic acid is 20-400 mu m/l and the concentration of methyl jasmonate is 20-400 mu m/l;
the weight ratio of the first component to the second component is 1-50: 50-1.
As a preferable scheme of the invention, the weight ratio of the component I to the component II is 1-25: 25-1.
In a preferred embodiment of the present invention, the pH of the aqueous solution of the mixture of the two components is 7.2 to 7.5.
The technical scheme of the second aspect of the invention is as follows: a preparation method of a soil improvement microbial agent comprises the following steps:
s1, sterilizing turf and diatomite in a certain proportion, and then preparing mixed mineral particles with a porous structure;
s2, mixing the fermentation stock solutions, adding chitosan and ganoderma lucidum polysaccharide, storing at the temperature of 30-40 ℃ for 20-30 hours, and mixing the fermentation stock solutions;
s3, preparing a mixed aqueous solution of oxoacid, methyl jasmonic acid and methyl jasmonate according to a certain proportion;
s4, spraying the remixed fermentation stock solution to the mixed mineral particles;
and S5, mixing the mixed aqueous solution obtained in the step S3 with the mixed mineral particles obtained in the step S4 to obtain the soil improvement microbial inoculum.
As a preferable embodiment of the present invention, the step 3 further comprises adjusting the pH of the mixed aqueous solution to 7.2 to 7.5.
As a preferable embodiment of the present invention, the step S5 is to spray the mixed aqueous solution of the step S3 onto the mixed mineral particles of the step S4 or to wet the mixed mineral particles of the step S4 into the mixed aqueous solution of the step S3.
The third aspect of the invention has the technical scheme that: application of the soil improvement microbial agent in planting of kiwi fruits or citrus aurantium.
The invention achieves the following beneficial effects:
1. obviously promoting the plant to be strong, having high fruit setting and maturing rate, uniform color, good surface gloss, gorgeous color, large and uniform fruit, high sugar degree and high yield.
2. The soil aggregate structure is increased, the soil fertility is improved, the acidity of the soil is reduced, the ecological environment of the soil is improved, the soil is prevented from hardening, the permeability is improved, and the soil and water are stored;
3. the spraying or coating treatment of the branches and leaves of the fruits or the fruits is not needed in the later period, and the fruit fragrance of the fruits can be increased only by fertilizing the roots of the fruit trees.
Detailed Description
A soil improvement microbial inoculant comprising the following components:
the component one: the composite material comprises the following raw materials in parts by weight: 5-10 parts of turf, 2-8 parts of diatomite, 1-5 parts of chitosan, 1-4 parts of ganoderma lucidum polysaccharide and 1 part of microbial agent, wherein the microbial agent is a microbial agent formed by independently fermenting microbial bacteria in a mixture, and the weight ratio of fermentation stock solutions of potassium bacteria, bacillus subtilis, lactobacillus acidophilus, bifidobacterium, actinomycetes, helicobacter and salmonella in the microbial agent is 1:0.5-3:0.5-3:0.5-3: 0.5-2: 0.5-3:0.5-2, wherein the effective amount of the microorganism in the microorganism agent is 1.2-1.4 hundred million/g;
and (2) component two: mixed water solution of oxy-water acid, methyl jasmonic acid and methyl jasmonate, wherein the concentration of salicylic acid is 20-400 mu m/l, the concentration of methyl jasmonic acid is 20-400 mu m/l and the concentration of methyl jasmonate is 20-400 mu m/l;
the weight ratio of the first component to the second component is 1-50: 50-1, more preferably 1-25: 25-1.
A preparation method of a soil improvement microbial agent comprises the following steps:
s1, sterilizing turf and diatomite in a certain proportion, and then preparing mixed mineral particles with a porous structure;
s2, mixing the fermentation stock solutions, adding chitosan and ganoderma lucidum polysaccharide, storing at the temperature of 30-40 ℃ for 20-30 hours, and mixing the fermentation stock solutions;
s3, preparing a mixed aqueous solution of oxyacid, methyl jasmonic acid and methyl jasmonate according to a certain proportion, and adjusting the pH value of the aqueous solution to 7.2-7.5;
s4, spraying the remixed fermentation stock solution to the mixed mineral particles;
s5, mixing the mixed aqueous solution obtained in the step S3 with the mixed mineral particles obtained in the step S4 to obtain a soil improvement microbial inoculum:
specifically, the mixed aqueous solution of the step S3 is sprayed onto the mixed mineral particles of the step S4, or the mixed mineral particles of the step S4 are soaked into the mixed aqueous solution of the step S3.
The application of the soil improvement microbial agent in the planting of kiwi fruits or citrus reticulata can promote the development of fruits, effectively prevent the undesirable development of fruit cracking, flower dropping, fruit shrinking and the like when the fruits bear fruits, improve the growth function of fruit trees and the development function of the fruits, stimulate more fragrant esters and fragrant aldehydes to be generated in the growth process of the fruits and increase the fragrance of the fruits.
To further illustrate the present invention, the following examples are provided for illustration.
Example 1:
a soil improvement microbial inoculant comprising the following components:
the component one: the composite material comprises the following raw materials in parts by weight: 5 parts of turf, 2 parts of diatomite, 1 part of chitosan, 1 part of ganoderma lucidum polysaccharide and 1 part of microbial agent, wherein the microbial agent is a microbial agent formed by independently fermenting microbial bacteria in a mixture, and the weight ratio of fermentation stock solutions of potassium bacteria, bacillus subtilis, lactobacillus acidophilus, bifidobacterium, actinomycetes, helicobacter and salmonella in the microbial agent is 1:0.5: 0.5: 0.5: 0.5:0.5, wherein the effective amount of the microorganism in the microorganism agent is 1.2 hundred million/g;
and (2) component two: a mixed aqueous solution of oxy-hydric acid, methyl jasmonic acid and methyl jasmonate, wherein the concentration of salicylic acid is 20 mu m/l, the concentration of methyl jasmonic acid is 400 mu m/l and the concentration of methyl jasmonate is 400 mu m/l;
the weight ratio of the first component to the second component is 50: 1.
The preparation method comprises the following steps:
s1, sterilizing the grass carbon and the diatomite in parts by weight, and preparing mixed mineral particles with porous structures;
s2, mixing the fermentation stock solutions, adding the chitosan and the ganoderma lucidum polysaccharide in parts by weight, storing at the temperature of 40 ℃ for 20 hours, and mixing the fermentation stock solutions;
s3, preparing a mixed aqueous solution of oxyacid, methyl jasmonic acid and methyl jasmonate according to a certain proportion, and adjusting the pH value of the aqueous solution to 7.2;
s4, spraying the remixed fermentation stock solution to the mixed mineral particles;
s5, mixing the mixed aqueous solution obtained in the step S3 with the mixed mineral particles obtained in the step S4 to obtain a soil improvement microbial inoculum: specifically, the mixed aqueous solution of the step S3 is sprayed to the mixed mineral particles of the step S4 and dried.
Example 2:
the component one: the composite material comprises the following raw materials in parts by weight: 5 parts of turf, 2 parts of diatomite, 1 part of chitosan, 1 part of ganoderma lucidum polysaccharide and 1 part of microbial agent, wherein the microbial agent is a microbial agent formed by independently fermenting microbial bacteria in a mixture, and the weight ratio of fermentation stock solutions of potassium bacteria, bacillus subtilis, lactobacillus acidophilus, bifidobacterium, actinomycetes, helicobacter and salmonella in the microbial agent is 1:2: 1:1: 1:1: 2, wherein the effective amount of the microorganism in the microorganism agent is 1.4 hundred million/g;
and (2) component two: a mixed aqueous solution of oxy-hydric acid, methyl jasmonic acid and methyl jasmonate, wherein the concentration of salicylic acid is 20 mu m/l, the concentration of methyl jasmonic acid is 400 mu m/l and the concentration of methyl jasmonate is 400 mu m/l;
the weight ratio of the first component to the second component is 25: 1.
The preparation method comprises the following steps:
s1, sterilizing the grass carbon and the diatomite in parts by weight, and preparing mixed mineral particles with porous structures;
s2, mixing the fermentation stock solutions, adding the chitosan and the ganoderma lucidum polysaccharide in parts by weight, storing at the temperature of 40 ℃ for 20 hours, and mixing the fermentation stock solutions;
s3, preparing a mixed aqueous solution of oxyacid, methyl jasmonic acid and methyl jasmonate according to a certain proportion, and adjusting the pH value of the aqueous solution to 7.4;
s4, spraying the remixed fermentation stock solution to the mixed mineral particles;
s5, mixing the mixed aqueous solution obtained in the step S3 with the mixed mineral particles obtained in the step S4 to obtain a soil improvement microbial inoculum: specifically, the mixed aqueous solution of the step S3 is sprayed to the mixed mineral particles of the step S4 and dried.
Example 3:
the component one: the composite material comprises the following raw materials in parts by weight: 5 parts of turf, 2 parts of diatomite, 1 part of chitosan, 1 part of ganoderma lucidum polysaccharide and 1 part of microbial agent, wherein the microbial agent is a microbial agent formed by independently fermenting microbial bacteria in a mixture, and the weight ratio of fermentation stock solutions of potassium bacteria, bacillus subtilis, lactobacillus acidophilus, bifidobacterium, actinomycetes, helicobacter and salmonella in the microbial agent is 1:1: 1:1: 1:1: 2, wherein the effective amount of the microorganism in the microorganism agent is 1.4 hundred million/g;
and (2) component two: a mixed aqueous solution of oxy-water acid, methyl jasmonic acid and methyl jasmonate, wherein the concentration of salicylic acid is 20 mu m/l, the concentration of methyl jasmonic acid is 20 mu m/l and the concentration of methyl jasmonate is 400 mu m/l;
the weight ratio of the first component to the second component is 25: 1.
The preparation method comprises the following steps:
s1, sterilizing the grass carbon and the diatomite in parts by weight, and preparing mixed mineral particles with porous structures;
s2, mixing the fermentation stock solutions, adding the chitosan and the ganoderma lucidum polysaccharide in parts by weight, storing at the temperature of 40 ℃ for 20 hours, and mixing the fermentation stock solutions;
s3, preparing a mixed aqueous solution of oxyacid, methyl jasmonic acid and methyl jasmonate according to a certain proportion, and adjusting the pH value of the aqueous solution to 7.4;
s4, spraying the remixed fermentation stock solution to the mixed mineral particles;
s5, mixing the mixed aqueous solution obtained in the step S3 with the mixed mineral particles obtained in the step S4 to obtain a soil improvement microbial inoculum: specifically, the mixed aqueous solution of the step S3 is sprayed to the mixed mineral particles of the step S4 and dried.
Example 4:
a soil improvement microbial inoculant comprising the following components:
the component one: the composite material comprises the following raw materials in parts by weight: 5 parts of turf, 2 parts of diatomite, 1 part of chitosan, 1 part of ganoderma lucidum polysaccharide and 1 part of microbial agent, wherein the microbial agent is a microbial agent formed by independently fermenting microbial bacteria in a mixture, and the weight ratio of fermentation stock solutions of potassium bacteria, bacillus subtilis, lactobacillus acidophilus, bifidobacterium, actinomycetes, helicobacter and salmonella in the microbial agent is 1:0.5: 0.5: 0.5: 0.5:0.5, wherein the effective amount of the microorganism in the microorganism agent is 1.2 hundred million/g;
and (2) component two: a mixed aqueous solution of oxy-hydric acid, methyl jasmonic acid and methyl jasmonate, wherein the concentration of salicylic acid is 20 mu m/l, the concentration of methyl jasmonic acid is 400 mu m/l and the concentration of methyl jasmonate is 400 mu m/l;
the weight ratio of the first component to the second component is 1: 50.
the preparation method comprises the following steps:
s1, sterilizing turf and diatomite in a certain proportion, and then preparing mixed mineral particles with a porous structure;
s2, mixing the fermentation stock solutions, adding chitosan and ganoderma lucidum polysaccharide, storing at the temperature of 30-40 ℃ for 20-30 hours, and mixing the fermentation stock solutions;
s3, preparing a mixed aqueous solution of oxyacid, methyl jasmonic acid and methyl jasmonate according to a certain proportion, and adjusting the pH value of the aqueous solution to 7.2-7.5;
s4, spraying the remixed fermentation stock solution to the mixed mineral particles;
s5, mixing the mixed aqueous solution obtained in the step S3 with the mixed mineral particles obtained in the step S4 to obtain a soil improvement microbial inoculum: specifically, the mixed aqueous solution of the step S3 is sprayed to the mixed mineral particles of the step S4, or the mixed mineral particles of the step S4 are soaked in the mixed aqueous solution of the step S3, and the mixed aqueous solution is dried after the soaking time is 3 hours.
Example 5:
the component one: the composite material comprises the following raw materials in parts by weight: 5 parts of turf, 2 parts of diatomite, 1 part of chitosan, 1 part of ganoderma lucidum polysaccharide and 1 part of microbial agent, wherein the microbial agent is a microbial agent formed by independently fermenting microbial bacteria in a mixture, and the weight ratio of fermentation stock solutions of potassium bacteria, bacillus subtilis, lactobacillus acidophilus, bifidobacterium, actinomycetes, helicobacter and salmonella in the microbial agent is 1:2: 1:1: 1:1: 2, wherein the effective amount of the microorganism in the microorganism agent is 1.4 hundred million/g;
and (2) component two: a mixed aqueous solution of oxy-hydric acid, methyl jasmonic acid and methyl jasmonate, wherein the concentration of salicylic acid is 20 mu m/l, the concentration of methyl jasmonic acid is 400 mu m/l and the concentration of methyl jasmonate is 400 mu m/l;
the weight ratio of the component one to the component two is 1: 20.
The preparation method comprises the following steps:
s1, sterilizing turf and diatomite in a certain proportion, and then preparing mixed mineral particles with a porous structure;
s2, mixing the fermentation stock solutions, adding chitosan and ganoderma lucidum polysaccharide, storing at the temperature of 30-40 ℃ for 20-30 hours, and mixing the fermentation stock solutions;
s3, preparing a mixed aqueous solution of oxyacid, methyl jasmonic acid and methyl jasmonate according to a certain proportion, and adjusting the pH value of the aqueous solution to 7.2-7.5;
s4, spraying the remixed fermentation stock solution to the mixed mineral particles;
s5, mixing the mixed aqueous solution obtained in the step S3 with the mixed mineral particles obtained in the step S4 to obtain a soil improvement microbial inoculum: specifically, the mixed aqueous solution of the step S3 is sprayed to the mixed mineral particles of the step S4, or the mixed mineral particles of the step S4 are soaked in the mixed aqueous solution of the step S3, and the mixed aqueous solution is dried after the soaking time is 3 hours.
Example 6:
the component one: the composite material comprises the following raw materials in parts by weight: 5 parts of turf, 2 parts of diatomite, 1 part of chitosan, 1 part of ganoderma lucidum polysaccharide and 1 part of microbial agent, wherein the microbial agent is a microbial agent formed by independently fermenting microbial bacteria in a mixture, and the weight ratio of fermentation stock solutions of potassium bacteria, bacillus subtilis, lactobacillus acidophilus, bifidobacterium, actinomycetes, helicobacter and salmonella in the microbial agent is 1:1: 1:1: 1:1: 2, wherein the effective amount of the microorganism in the microorganism agent is 1.4 hundred million/g;
and (2) component two: a mixed aqueous solution of oxy-water acid, methyl jasmonic acid and methyl jasmonate, wherein the concentration of salicylic acid is 20 mu m/l, the concentration of methyl jasmonic acid is 20 mu m/l and the concentration of methyl jasmonate is 400 mu m/l;
the weight ratio of the first component to the second component is 25: 1.
The preparation method comprises the following steps:
s1, sterilizing turf and diatomite in a certain proportion, and then preparing mixed mineral particles with a porous structure;
s2, mixing the fermentation stock solutions, adding chitosan and ganoderma lucidum polysaccharide, storing at the temperature of 30-40 ℃ for 20-30 hours, and mixing the fermentation stock solutions;
s3, preparing a mixed aqueous solution of oxyacid, methyl jasmonic acid and methyl jasmonate according to a certain proportion, and adjusting the pH value of the aqueous solution to 7.2-7.5;
s4, spraying the remixed fermentation stock solution to the mixed mineral particles;
s5, mixing the mixed aqueous solution obtained in the step S3 with the mixed mineral particles obtained in the step S4 to obtain a soil improvement microbial inoculum: specifically, the mixed aqueous solution of the step S3 is sprayed to the mixed mineral particles of the step S4, or the mixed mineral particles of the step S4 are soaked in the mixed aqueous solution of the step S3, and the mixed aqueous solution is dried after the soaking time is 3 hours.
Comparative example 1:
this comparative example differs from example 2 in that:
no chitosan was added.
Comparative example 2:
this comparative example differs from example 2 in that:
no ganoderan was added.
Comparative example 3:
this comparative example differs from example 2 in that:
no methyl jasmonic acid was added.
Taking kiwi fruits as an example, respectively adopting a ditch application method (each example aims at 2205 kiwi fruits grafted by a cleft grafting method equivalent to a 32-degree north latitude growing environment) in the examples 2, 5 and 1-3, fertilizing the fertilizer with the depth of 5cm, covering soil after fertilizing, and watering thoroughly after covering the soil. Fertilizing for 2 times, wherein the weight of the fertilizer applied to the red poplar of the kiwi fruit branches just grafted one month is 3 kg/plant, and the weight of the fertilizer applied to the adult fruit tree trees in the flowering period of the fourth year is 4.5 kg/plant.
Picking fresh kiwi fruits within one day after first kiwi fruit fruiting in the fourth year, and performing related tests, wherein ethyl 3-hydroxybutyrate and hexenal are characteristic aroma components occupying more aroma components, and the test structures are as shown in the following table, as shown in table 1:
TABLE 1
Figure BDA0003387535310000061
Figure BDA0003387535310000071
The flavor ranking was performed by human sensory evaluation (test group members were 9), and the ranking is shown in table 2:
TABLE 2
Item Example 2 Example 5 Comparative example 1 Comparative example 2 Comparative example 3
Sort 1 st vote count 0 9 0 0 0
Sort the 2 nd number of votes 8 0 0 1 0
Sort the 3 rd number of votes 1 0 0 8 0
Sort the 4 th vote 0 0 9 0 0
Sort 5 th vote count 0 0 0 0 9
From tables 1 and 2, it can be seen that component one and component two have synergistic effects, improving the activity of the soil and increasing the yield and quality of kiwi fruits. The applicant believes that the methyl jasmonate has a bactericidal effect, the influence of the immune methyl jasmonate of the microbial strains can be protected by combining various microbial strains and adding the chitosan and the ganoderan, and more fragrant esters and fragrant aldehydes are generated in the growth process of the kiwi fruit under the combined action of the water-oxygen acid, the methyl jasmonate and the methyl jasmonate, so that the fruit fragrance of the kiwi fruit is stronger.
Technical solutions in the embodiments of the present invention are clearly and completely described, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The present invention is described in detail in order to make those skilled in the art understand the content and practice the invention, and the invention is not limited to the above embodiments, and all equivalent changes or modifications made according to the spirit of the invention should be covered by the scope of the invention.

Claims (7)

1. A soil improvement microbial agent, which is characterized in that: comprises the following components:
the component one: the composite material comprises the following raw materials in parts by weight: 5-10 parts of turf, 2-8 parts of diatomite, 1-5 parts of chitosan, 1-4 parts of ganoderma lucidum polysaccharide and 1 part of microbial agent, wherein the microbial agent is a microbial agent formed by independently fermenting microbial bacteria in a mixture, and the weight ratio of fermentation stock solutions of potassium bacteria, bacillus subtilis, lactobacillus acidophilus, bifidobacterium, actinomycetes, helicobacter and salmonella in the microbial agent is 1:0.5-3:0.5-3:0.5-3: 0.5-2: 0.5-3:0.5-2, wherein the effective amount of the microorganism in the microorganism agent is 1.2-1.4 hundred million/g;
and (2) component two: mixed water solution of oxy-water acid, methyl jasmonic acid and methyl jasmonate, wherein the concentration of salicylic acid is 20-400 mu m/l, the concentration of methyl jasmonic acid is 20-400 mu m/l and the concentration of methyl jasmonate is 20-400 mu m/l;
the weight ratio of the first component to the second component is 1-50: 50-1.
2. The soil improvement microbial inoculant of claim 1, wherein: the weight ratio of the first component to the second component is 1-25: 25-1.
3. The soil improvement microbial inoculant of claim 1, wherein: the pH value of the mixed aqueous solution of the component two is 7.2-7.5.
4. A method of preparing a soil improving microbial inoculant according to any one of claims 1 to 3 wherein: the method comprises the following steps:
s1, sterilizing turf and diatomite in a certain proportion, and then preparing mixed mineral particles with a porous structure;
s2, mixing the fermentation stock solutions, adding chitosan and ganoderma lucidum polysaccharide, storing at the temperature of 30-40 ℃ for 20-30 hours, and mixing the fermentation stock solutions;
s3, preparing a mixed aqueous solution of oxyacid, methyl jasmonic acid and methyl jasmonate according to a certain proportion;
s4, spraying the remixed fermentation stock solution to the mixed mineral particles;
and S5, mixing the mixed aqueous solution obtained in the step S3 with the mixed mineral particles obtained in the step S4 to obtain the soil improvement microbial inoculum.
5. The method for preparing a soil improvement microbial inoculum according to claim 4, wherein the method comprises the following steps: the step 3 also comprises adjusting the pH of the mixed aqueous solution to 7.2-7.5.
6. The method for preparing a soil-improving microbial inoculant according to claim 4 or 5, wherein:
the step of S5 is specifically to spray the mixed aqueous solution of the step of S3 to the mixed mineral particles of S4 or to wet the mixed mineral particles of S4 to the mixed aqueous solution of the step of S3.
7. The use of a soil improving microbial inoculant according to claim 1 for the planting of kiwi fruit or citrus reticulata.
CN202111461218.3A 2021-12-01 2021-12-01 Soil improvement microbial agent and preparation method thereof Pending CN113956101A (en)

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Application publication date: 20220121