CN113952792A - Material for negative pressure purification and toxin filtration and preparation method thereof - Google Patents

Material for negative pressure purification and toxin filtration and preparation method thereof Download PDF

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Publication number
CN113952792A
CN113952792A CN202010619767.8A CN202010619767A CN113952792A CN 113952792 A CN113952792 A CN 113952792A CN 202010619767 A CN202010619767 A CN 202010619767A CN 113952792 A CN113952792 A CN 113952792A
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Prior art keywords
silane coupling
glass fiber
reaction
coupling agent
lysozyme
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CN202010619767.8A
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Chinese (zh)
Inventor
李秀文
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Shandong Zhonghao Holdings Group Co ltd
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Shandong Zhonghao Holdings Group Co ltd
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Priority to CN202010619767.8A priority Critical patent/CN113952792A/en
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D39/00—Filtering material for liquid or gaseous fluids
    • B01D39/14—Other self-supporting filtering material ; Other filtering material
    • B01D39/16—Other self-supporting filtering material ; Other filtering material of organic material, e.g. synthetic fibres
    • B01D39/1607—Other self-supporting filtering material ; Other filtering material of organic material, e.g. synthetic fibres the material being fibrous
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D46/00—Filters or filtering processes specially modified for separating dispersed particles from gases or vapours
    • B01D46/0001—Making filtering elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D46/00—Filters or filtering processes specially modified for separating dispersed particles from gases or vapours
    • B01D46/0027—Filters or filtering processes specially modified for separating dispersed particles from gases or vapours with additional separating or treating functions
    • B01D46/0028—Filters or filtering processes specially modified for separating dispersed particles from gases or vapours with additional separating or treating functions provided with antibacterial or antifungal means
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M13/00—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment
    • D06M13/10—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment with compounds containing oxygen
    • D06M13/12—Aldehydes; Ketones
    • D06M13/123—Polyaldehydes; Polyketones
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M13/00—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment
    • D06M13/50—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with non-macromolecular organic compounds; Such treatment combined with mechanical treatment with organometallic compounds; with organic compounds containing boron, silicon, selenium or tellurium atoms
    • D06M13/51—Compounds with at least one carbon-metal or carbon-boron, carbon-silicon, carbon-selenium, or carbon-tellurium bond
    • D06M13/513—Compounds with at least one carbon-metal or carbon-boron, carbon-silicon, carbon-selenium, or carbon-tellurium bond with at least one carbon-silicon bond
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
    • D06M16/003—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Textile Engineering (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Inorganic Chemistry (AREA)
  • Organic Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Filtering Materials (AREA)
  • Treatments For Attaching Organic Compounds To Fibrous Goods (AREA)

Abstract

Discloses a material for negative pressure purification and toxin filtration, which comprises a glass fiber layered material and lysozyme; the glass fiber is selected from glass fiber modified by silane coupling agent and aldehydic agent; the silane coupling agent is selected from diamine type silane coupling agents. In addition, a method for preparing the material for negative pressure purification and toxin filtration is also disclosed. The material of the invention not only obviously improves the antibacterial rate of common staphylococcus aureus, but also does not obviously reduce the antibacterial effect after long-time storage or use.

Description

Material for negative pressure purification and toxin filtration and preparation method thereof
Technical Field
The invention belongs to the technical field of air purification, and relates to a material for negative pressure purification and toxin filtration and a preparation method thereof.
Background
Along with the continuous development of economic society, the demand of each country for the negative pressure purification and toxicity filtration device is more and more urgent. The negative pressure purification and poison filtering device can ensure that the environment in the device is not influenced by the outside polluted air, and simultaneously can ensure that the air in the device cannot pollute the outside environment, thereby being beneficial to protecting the health and life safety of the public.
Generally speaking, the negative pressure purifying and toxin filtering device comprises an air isolation system, a purifying and toxin filtering system, a negative pressure generating system and a control and detection system. Under the working state, the internal pressure of the negative pressure purification and toxicity filtration device is lower than the external atmospheric pressure, and air can enter and exit through strict filtration and purification. The device is mainly used for preventing pathogenic microorganisms from spreading through aerosol and preventing the pathogenic microorganisms from polluting the atmospheric environment.
However, most of the purification and toxicity-filtering systems can only block particulate matters, bacteria, viruses and the like in the air through the filtering material, and can not effectively kill microorganisms accumulated in the purification and toxicity-filtering systems. Along with the continuous accumulation of time, microorganisms can breed in a large number in the purification and toxicity filtering system, and further pollution is caused along with air supply. In order to solve the above problems, people mainly use filtration sterilization, electret sterilization, photocatalytic sterilization, plasma sterilization, ultraviolet radiation, bactericide or bacteriostatic agent modification and other means to modify the system for purifying and filtering toxins.
Haolimei et al select the highly safe biological antibacterial agent lysozyme and the commonly used filter material glass fiber as the base material, and use the chemical method to modify it, thus fix the biological antibacterial agent on the glass fiber by the way of covalent bond. However, the antibacterial rate of the antibacterial agent to common escherichia coli and staphylococcus aureus is about 80%, and the antibacterial effect is obviously reduced after long-time storage or use.
Therefore, in view of the above technical defects, there is an urgent need for improvement of the existing material for negative pressure purification and filtration and the preparation method thereof.
Disclosure of Invention
The invention aims to provide a material for negative pressure purification and toxin filtration and a preparation method thereof. The antibacterial material not only obviously improves the antibacterial rate of common staphylococcus aureus, but also has no obvious reduction of the antibacterial effect after long-time storage or use.
In order to achieve the above object, in one aspect, the technical solution adopted by the present invention is as follows:
a material for negative pressure purification and toxin filtration, which comprises a glass fiber layered material and lysozyme; the glass fiber is selected from glass fibers modified by a silane coupling agent and an aldehydic agent; characterized in that the silane coupling agent is selected from a diamine type silane coupling agent.
The material according to the invention, wherein the silane coupling agent is selected from KH-602 and KH-843.
The material according to the invention, wherein the aldehyde-forming agent is selected from glutaraldehyde.
The material according to the invention, wherein the lysozyme is selected from egg white lysozyme.
On the other hand, the invention also provides a preparation method of the material for negative pressure purification and toxin filtration, which comprises the steps of placing the glass fiber layered material in a toluene solution of a silane coupling agent, and carrying out coupling reaction under oscillation; drying, placing the mixture into an aldehydic agent aqueous solution, performing an aldehydic reaction under an oscillation condition, taking out the mixture, and rinsing the mixture with deionized water; and then placing the pretreated glass fiber layered material in a phosphate buffer solution of lysozyme to perform enzyme immobilization reaction, washing by using the phosphate buffer solution after the reaction, and drying.
The method of the invention, wherein the average diameter of the glass fiber is 400-700 nm; the thickness of the glass fiber layered material is 0.2-1.0 mm.
The method of the invention, wherein the concentration of the silane coupling agent is 8-12 wt%; the time of the coupling reaction is 4-24 h.
The method of the invention, wherein the concentration of the aldehyding agent is 0.5-1.5 wt%; the time of the aldehyde reaction is 4-24 h.
The method of the invention, wherein the concentration of the lysozyme is 2-10 mg/mL; the pH value of the phosphate buffer solution is 6-8.
The method of the invention, wherein the temperature of the enzyme immobilization reaction is 4-10 ℃, and the reaction time is 6-15 h.
Compared with the material obtained by the monoamino silane coupling agent, the material for negative pressure purification and toxin filtration provided by the invention has the beneficial technical effects that the antibacterial rate of the material for negative pressure purification and toxin filtration is obviously improved for common staphylococcus aureus, and the antibacterial effect is not obviously reduced after long-time storage or use.
Detailed Description
The invention will be further illustrated with reference to specific embodiments.
Example 1
Placing the glass fiber layered material in 10 wt% of KH-602 (N-beta- (aminoethyl) -gamma-aminopropylmethyldimethoxysilane) toluene solution, and carrying out coupling reaction under the oscillation condition for 8 h; the average diameter of the glass fiber is 56 nm; the thickness of the glass fiber layered material is 0.5 mm; drying, placing the mixture into a glutaraldehyde aqueous solution, and carrying out an hydroformylation reaction under the oscillation condition for 6 hours; taking out and rinsing with deionized water; then placing the pretreated glass fiber layered material into a phosphate buffer (pH 7) of 5mg/mL egg white lysozyme to carry out enzyme immobilization reaction, wherein the temperature of the enzyme immobilization reaction is 6 ℃, and the reaction time is 10 hours; after the reaction, the reaction was washed 3 times with the phosphate buffer (pH 7) and dried to obtain the product.
Example 2
The silane coupling agent for coupling reaction was replaced with a bisamino type silane coupling agent KH-843 (N-. beta. - (aminoethyl) - γ -aminopropyltriethoxysilane), and the other conditions were the same as in example 1.
Comparative example 1
The silane coupling agent of the coupling reaction was replaced with a monoamino type silane coupling agent KH-550, and the other conditions were the same as in example 1.
Performance testing
A bacterial suspension of Staphylococcus aureus (ATCC 6538) was prepared according to the method of Helimei et al (journal of preventive medicine of the military, 29(1), P14), at a concentration of 1.0X 106-5.0×106cfu/ml. Then, the antibacterial performance of the products of example 1 and comparative example 1 was evaluated according to the sterilization evaluation test (droplet vapor phase test method) of Japanese Standard NU 734-99-Lysozyme filter paper. In addition, the long-term efficacy test was conducted with reference to the stability test of the disinfection specification (2002 edition). The products of example 1 and comparative example 1 were stored at 37 ℃ for 3 months, and the antibacterial performance was measured according to the method of 1.4.
See table 1 for results.
TABLE 1
Figure BDA0002564780870000031
Figure BDA0002564780870000041
As can be seen from table 1, compared with the materials for negative pressure decontamination and toxin filtration according to examples 1 and 2 of the present invention, the antibacterial rate of the materials for negative pressure decontamination and toxin filtration is significantly increased (more than 92%) against common staphylococcus aureus, and the antibacterial effect is not significantly reduced (only about 4%) after long-term storage or use. As a comparison, comparative example 1, which is a monoamino type silane coupling agent KH-550, gave materials of 78.24% and 13.7%, respectively.
Without wishing to be bound by any theory, the use of bis-monoamino silane coupling agents results in products with higher lysozyme activity and better immobilization of the surface than monoamino silane coupling agents.
It should be understood that the detailed description of the invention is merely illustrative of the spirit and principles of the invention and is not intended to limit the scope of the invention. Furthermore, it should be understood that various changes, substitutions, deletions, modifications or adjustments may be made by those skilled in the art after reading the disclosure of the present invention, and such equivalents are also within the scope of the invention as defined in the appended claims.

Claims (10)

1. A material for negative pressure purification and toxin filtration, which comprises a glass fiber layered material and lysozyme; the glass fiber is selected from glass fibers modified by a silane coupling agent and an aldehydic agent; characterized in that the silane coupling agent is selected from a diamine type silane coupling agent.
2. The material of claim 1, wherein the silane coupling agent is selected from KH-602 and KH-843.
3. The material of claim 1, wherein the aldehyde-forming agent is selected from glutaraldehyde.
4. The material of claim 1, wherein said lysozyme is selected from egg white lysozyme.
5. A method for preparing the material of any one of claims 1 to 4, which comprises placing a glass fiber layered material in a toluene solution of a silane coupling agent, and carrying out a coupling reaction under shaking conditions; drying, placing the mixture into an aldehydic agent aqueous solution, performing an aldehydic reaction under an oscillation condition, taking out the mixture, and rinsing the mixture with deionized water; and then placing the pretreated glass fiber layered material in a phosphate buffer solution of lysozyme to perform enzyme immobilization reaction, washing by using the phosphate buffer solution after the reaction, and drying.
6. The method as claimed in claim 5, wherein the glass fiber has an average diameter of 400-700 nm; the thickness of the glass fiber layered material is 0.2-1.0 mm.
7. The method of claim 5, wherein the concentration of the silane coupling agent is 8-12 wt%; the time of the coupling reaction is 4-24 h.
8. The method of claim 5, wherein the concentration of the aldehyde reagent is 0.5-1.5 wt%; the time of the aldehyde reaction is 4-24 h.
9. The method according to claim 5, wherein the concentration of lysozyme is 2-10 mg/mL; the pH value of the phosphate buffer solution is 6-8.
10. The method according to claim 5, wherein the temperature of the enzyme immobilization reaction is 4-10 ℃ and the reaction time is 6-15 hours.
CN202010619767.8A 2020-07-01 2020-07-01 Material for negative pressure purification and toxin filtration and preparation method thereof Pending CN113952792A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5897955A (en) * 1996-06-03 1999-04-27 Gore Hybrid Technologies, Inc. Materials and methods for the immobilization of bioactive species onto polymeric substrates
CN1229366A (en) * 1996-07-25 1999-09-22 日挥通用株式会社 Air cleaning filter
CN101293107A (en) * 2008-06-13 2008-10-29 中国人民解放军军事医学科学院卫生装备研究所 Broad spectrum biological disinfection air filtering material and preparation method thereof
CN109439189A (en) * 2018-11-21 2019-03-08 中国船舶重工集团公司第七二五研究所 A kind of process for fixation of antifouling enzyme on inorganic coating surface

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5897955A (en) * 1996-06-03 1999-04-27 Gore Hybrid Technologies, Inc. Materials and methods for the immobilization of bioactive species onto polymeric substrates
CN1229366A (en) * 1996-07-25 1999-09-22 日挥通用株式会社 Air cleaning filter
CN101293107A (en) * 2008-06-13 2008-10-29 中国人民解放军军事医学科学院卫生装备研究所 Broad spectrum biological disinfection air filtering material and preparation method thereof
CN109439189A (en) * 2018-11-21 2019-03-08 中国船舶重工集团公司第七二五研究所 A kind of process for fixation of antifouling enzyme on inorganic coating surface

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