CN113951130A - Porphyra haitanensis somatic cell filament seedling transplantation shell seedling collection method - Google Patents
Porphyra haitanensis somatic cell filament seedling transplantation shell seedling collection method Download PDFInfo
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- CN113951130A CN113951130A CN202010706934.2A CN202010706934A CN113951130A CN 113951130 A CN113951130 A CN 113951130A CN 202010706934 A CN202010706934 A CN 202010706934A CN 113951130 A CN113951130 A CN 113951130A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G33/00—Cultivation of seaweed or algae
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The invention discloses a porphyra haitanensis somatic cell filament seedling transplanting shell seedling collecting method, which comprises the following steps: in the seedling picking season, stringing shells into a plurality of strings of shell strings; laying a layer of shell strings at the bottom of the water pool, and meanwhile, arranging bamboo poles on which the shell strings are hung at intervals at the top of the water pool; cutting the porphyra haitanensis somatic cell filament seedlings into algae sections by a homogenizer, adding seawater for dilution, and uniformly spraying the algae sections in a water pool by a spray can, wherein the algae sections can climb on the bottom of the pool or on a suspended shell; performing microscopic examination after 15-20 days, when the average number of 3-6 plants in each 10 × 10 visual field of the upper hanging part reaches, meeting the seedling collecting requirement, and removing a part of the hung shell string to a partition empty pool for culture; then hanging the shell strings spread at the bottom of the pool under a bamboo pole for culturing, and when each 10 multiplied by 10 visual field reaches 3-6 strains on average, the requirement of seedling collection is met. By adopting the method, the laver seedling collection efficiency can be improved, the management of the seedling collection pool is simplified, and the labor cost is saved.
Description
Technical Field
The invention relates to the technical field of laver culture, in particular to a method for collecting a shell seedling by transplanting a somatic cell filament seedling of porphyra haitanensis.
Background
Porphyra haitanensis is a seaweed which is unique in China and can be artificially cultivated. The porphyra haitanensis is delicious and cheap, has rich resources, contains a large amount of amino acids, mineral substances and vitamins which are necessary for human bodies, is a nutritional health food with extremely high grade, and is a good name of 'nutrition treasury'.
The traditional porphyra haitanensis somatic cell filament seedling picking mode is that shells are tiled at the bottom of the pool, and the mode is large in labor amount, small in unit seedling emergence amount, time-consuming and labor-consuming, and high in labor cost expenditure, and is not beneficial to large-scale production.
Disclosure of Invention
The invention aims to provide a porphyra haitanensis somatic cell filament seedling transplanting shell seedling collecting method capable of improving laver seedling collecting efficiency, simplifying management of a seedling collecting pool and saving labor cost
In order to achieve the purpose, the technical scheme of the invention is as follows:
a porphyra haitanensis somatic cell filament seedling transplanting shell seedling collecting method comprises the following steps:
1) in the seedling picking season, the specific gravity of the water in the indoor water pool is 1.018-1.021, and the temperature of the water in the pool is 15-18 ℃;
2) stringing the shells into a plurality of strings of shell strings;
3) laying a layer of shell strings at the bottom of the water pool, and meanwhile, arranging bamboo poles on which the shell strings are hung at intervals at the top of the water pool;
4) cutting Porphyra haihanensis somatic cell filament seedling into 0.045-0.055 mm segments by a homogenizer, adding seawater to dilute, and uniformly spraying in a water pool by a spray can, wherein the segments can climb to the bottom of the pool or on a suspended shell;
5) performing microscopic examination after 15-20 days, when the average 3-6 plants in each 10 × 10 visual field of the upper hanging part reach, achieving the seedling collection requirement, and removing a part of the hung shell string to a separated empty pool for culture (when seedling is collected, the flat seedlings at the bottom of the pool are hung up for culture, so that an empty pool is arranged beside each two pools);
6) then hanging the shell strings spread at the bottom of the pool under a bamboo pole for culturing, and when each 10 multiplied by 10 visual field reaches 3-6 strains on average, the requirement of seedling collection is met.
Further, in the step 1), the seedling picking season is 1-3 months per year.
Further, in the step 2), each string of shells is 10 layers, and each layer is 2 shells.
Further, in the step 2), the length of the shell string is smaller than the height of the water pool.
Further, in the step 2), the shell is a clam shell with the diameter of 5-7 cm.
In the step 3), the bamboo poles are transversely arranged in parallel to the water pool, and the interval between adjacent parallelly arranged bamboo poles is 8-10 cm.
Further, in the step 3), the putting amount of the algae segments is 2-2.5 hundred million algae segments/1 m3A body of water.
Furthermore, the illumination intensity of the upper end of the shell string which is hung and cultured is greater than that of the lower end of the shell string, and after the shell string is cultured for a period of time, the shell string is turned over for culture, so that the growth conditions of the laver at the two ends of the shell string are consistent as much as possible.
Similarly, the shell strings spread on the bottom of the pool are hung up and cultured for a period of time, and then are turned around for culture.
According to the technical scheme, the shell strings are paved at the bottom of the water pool, and the bamboo poles are arranged at the top of the water pool for hanging the shell strings, so that the unit seedling emergence amount of the laver can be increased, the bamboo poles arranged at the top of the water pool can prevent laver somatic cell filaments in the pool from floating, and meanwhile, the bamboo poles can shield partial light intensity. Because the illumination intensity received at the two ends of the hung shell string is different, the hung shell string is required to be turned around for culture after being cultured for a certain time. By adopting the method, the laver seedling collection efficiency can be improved, the management of the seedling collection pool is simplified, and the labor cost is saved.
Detailed Description
A porphyra haitanensis somatic cell filament seedling transplanting shell seedling collecting method comprises the following steps:
1) in the seedling picking season (1-3 months per year), the specific gravity of the pond water of the indoor pond is 1.018-1.021, and the temperature of the pond water is 15-18 ℃;
2) stringing clam shells with the diameter of 5-7cm into a plurality of strings of shell strings, wherein each string of shell strings comprises 10 layers and 2 shells on each layer, and the length of each shell string is less than the height of the water pool;
3) laying a layer of shell strings at the bottom of the pool, and meanwhile, arranging bamboo poles on which the shell strings are hung at intervals at the top of the pool, wherein the bamboo poles are arranged in parallel with the pool in the transverse direction, and the interval between adjacent bamboo poles arranged in parallel is 8-10 cm;
4) cutting Porphyra haitanensis somatic cell filament seedling into 0.045-0.055 mm segments by homogenizer, diluting with seawater, spraying onto pool with spraying can, allowing the segments to climb to the bottom of the pool or on suspended shell, and putting 2-2.5 hundred million segments/1 m3A body of water;
5) performing microscopic examination after 15-20 days, and when the average number of 3-6 plants in each 10 × 10 visual field of the upper hanging part is reached, meeting the seedling picking requirement; removing a part of the shell string which is hung to a partition empty pool for culturing;
6) then hanging the shell strings spread at the bottom of the pool under a bamboo pole for culturing, and when each 10 multiplied by 10 visual field reaches 3-6 strains on average, the requirement of seedling collection is met.
In the process, because the illumination at the two ends of the shell string is different, the hung shell string is hung on the bamboo pole after being cultured for a certain time and turned around.
Regarding the selection of the amount of algal pieces put, the present invention has carried out the following screening test:
the suspension culture seedling collection experiment of the laver filament transplanted shell is carried out in five indoor cement ponds with 10 multiplied by 1.8 multiplied by 0.7m, the influence of different algae section dosage on the adhesion and germination of the algae filament transplanted shell is summarized, and the inspection result after 20 days is shown in table 1.
TABLE 1 algal dose billion/m3Influence unit on adhesion of suspended shell in phycofilament transplantation: Strain/10X 10
| The amount of algae | 1.2 | 1.5 | 1.8 | 2 | 2.5 |
| Upper end of | 0 | 1.8 | 3.2 | 8.2 | 7.9 |
| Middle part | 0 | 0.8 | 2.1 | 7 | 7.1 |
| Lower end | 0 | 0 | 0.9 | 4.8 | 4.2 |
Because the illumination intensity at the two ends and the middle part of the shell string hung under the bamboo pole is different, statistics is respectively carried out for each time, the average value of 10 visual fields is taken under a lens of 10 times of the germination quantity count, and the use amount is 1m3The amount of the algae put into the water body is billion. From Table 1, it can be seen that the seedling number of shell harvest after the phycofilament transplantation is 2 hundred million segments/1 m3Preferably, 2.5 hundred million segments/1 m3The next time.
The amount of the added algae segments is 2-2.5 hundred million segments/1 m3The water body is described in the specific embodiment.
Example 1
A porphyra haitanensis somatic cell filament seedling transplanting shell seedling collecting method comprises the following steps:
1) in the seedling picking season (1-3 months per year), the specific gravity of the water in the indoor water pool is 1.018, and the temperature of the water in the indoor water pool is 15 ℃;
2) stringing clam shells with the diameter of 5-7cm into a plurality of strings of shell strings, wherein each string of shell strings comprises 10 layers and 2 shells on each layer, and the length of each shell string is less than the height of the water pool;
3) laying a layer of shell strings at the bottom of the pool, and meanwhile, arranging bamboo poles on which the shell strings are hung at intervals at the top of the pool, wherein the bamboo poles are arranged in parallel with the pool in the transverse direction, and the interval between adjacent bamboo poles arranged in parallel is 8 cm;
4) cutting Porphyra haitanensis somatic cell filament seedlings into 0.045 mm sections by a homogenizer, adding seawater for dilution, uniformly spraying the sections in a water pool by a spray can, wherein the sections can climb to the bottom of the pool or hung shells, and the putting amount of the sections is 2 hundred million sections/1 m3A body of water;
5) performing microscopic examination after 15 days, and when each 10 multiplied by 10 visual field of the upper hanging part reaches 3 plants on average, meeting the seedling picking requirement; removing a part of the shell string which is hung to a partition empty pool for culturing;
6) then hanging the shell strings spread at the bottom of the pool under a bamboo pole for culturing, and when each 10 multiplied by 10 visual field reaches 3 strains on average, the requirement of seedling collection is met.
Example 2
A porphyra haitanensis somatic cell filament seedling transplanting shell seedling collecting method comprises the following steps:
1) in the seedling picking season (1-3 months per year), the specific gravity of the water in the indoor water pool is 1.021, and the temperature of the water in the indoor water pool is 18 ℃;
2) stringing clam shells with the diameter of 5-7cm into a plurality of strings of shell strings, wherein each string of shell strings comprises 10 layers and 2 shells on each layer, and the length of each shell string is less than the height of the water pool;
3) laying a layer of shell strings at the bottom of the pool, and meanwhile, arranging bamboo poles on which the shell strings are hung at intervals at the top of the pool, wherein the bamboo poles are arranged in parallel with the pool in the transverse direction, and the interval between adjacent bamboo poles arranged in parallel is 10 cm;
4) cutting Porphyra haitanensis somatic cell filament seedlings into 0.055 mm segments by a homogenizer, diluting with seawater, spraying uniformly onto a pool with a spray can, allowing the segments to climb to the bottom of the pool or on suspended shell, and adding 2.5 hundred million segments/1 m of segments3A body of water;
5) performing microscopic examination after 20 days, and when each 10 multiplied by 10 visual field of the upper hanging part reaches 6 plants on average, meeting the seedling picking requirement; removing a part of the shell string which is hung to a partition empty pool for culturing;
6) then hanging the shell strings spread at the bottom of the pool under a bamboo pole for culturing, and when each 10 multiplied by 10 visual field reaches 6 strains on average, the requirement of seedling collection is met.
Example 3
A porphyra haitanensis somatic cell filament seedling transplanting shell seedling collecting method comprises the following steps:
1) in the seedling picking season (1-3 months per year), the specific gravity of the water in the indoor water tank is 1.019, and the temperature of the water in the indoor water tank is 16.5 ℃;
2) stringing clam shells with the diameter of 5-7cm into a plurality of strings of shell strings, wherein each string of shell strings comprises 10 layers and 2 shells on each layer, and the length of each shell string is less than the height of the water pool;
3) laying a layer of shell strings at the bottom of the pool, and meanwhile, arranging bamboo poles on which the shell strings are hung at intervals at the top of the pool, wherein the bamboo poles are arranged in parallel with the pool in the transverse direction, and the interval between adjacent bamboo poles arranged in parallel is 9 cm;
4) cutting Porphyra haitanensis somatic cell filament seedlings into 0.05 mm sections by a homogenizer, adding seawater for dilution, uniformly spraying the sections in a water pool by a spray can, wherein the sections can climb to the bottom of the pool or hung on a shell, and the putting amount of the sections is 2.25 hundred million sections of sections/1 m3A body of water;
5) performing microscopic examination after 18 days, and when each 10 multiplied by 10 visual field of the upper hanging part reaches 5 plants on average, meeting the seedling picking requirement; removing a part of the shell string which is hung to a partition empty pool for culturing;
6) then hanging the shell strings spread at the bottom of the pool under a bamboo pole for culturing, and when each 10 multiplied by 10 visual field reaches 5 strains on average, the requirement of seedling collection is met.
Claims (10)
1. A porphyra haitanensis somatic cell filament seedling transplanting shell seedling collecting method is characterized in that: which comprises the following steps:
1) in the seedling picking season, the specific gravity of the water in the indoor water pool is 1.018-1.021, and the temperature of the water in the pool is 15-18 ℃;
2) stringing the shells into a plurality of strings of shell strings;
3) laying a layer of shell strings at the bottom of the water pool, and meanwhile, arranging bamboo poles on which the shell strings are hung at intervals at the top of the water pool;
4) cutting Porphyra haihanensis somatic cell filament seedling into 0.045-0.055 mm segments by a homogenizer, adding seawater to dilute, and uniformly spraying in a water pool by a spray can, wherein the segments can climb to the bottom of the pool or on a suspended shell;
5) performing microscopic examination after 15-20 days, when the average number of 3-6 plants in each 10 × 10 visual field of the upper hanging part reaches, meeting the seedling collecting requirement, and removing a part of the hung shell string to a partition empty pool for culture;
6) then hanging the shell strings spread at the bottom of the pool under a bamboo pole for culturing, and when each 10 multiplied by 10 visual field reaches 3-6 strains on average, the requirement of seedling collection is met.
2. The porphyra haitanensis somatic cell filament seedling transplantation shell seedling collecting method according to claim 1, which is characterized in that: in the step 1), the seedling picking season is 1-3 months per year.
3. The porphyra haitanensis somatic cell filament seedling transplantation shell seedling collecting method according to claim 1, which is characterized in that: in the step 2), each string of shells is 10 layers, and each layer is 2 shells.
4. The porphyra haitanensis somatic cell filament seedling transplantation shell seedling collecting method according to claim 1, which is characterized in that: in the step 2), the length of the shell string is smaller than the height of the water pool.
5. The porphyra haitanensis somatic cell filament seedling transplantation shell seedling collecting method according to claim 1, which is characterized in that: in the step 2), the shell is a clam shell with the diameter of 5-7 cm.
6. The porphyra haitanensis somatic cell filament seedling transplantation shell seedling collecting method according to claim 1, which is characterized in that: and 3), transversely discharging the bamboo poles parallel to the water pool.
7. The Porphyra haitanensis somatic cell filament seedling transplantation shell seedling collecting method according to claim 6, characterized in that: the interval between adjacent parallel bamboo poles is 8-10 cm.
8. The porphyra haitanensis somatic cell filament seedling transplantation shell seedling collecting method according to claim 1, which is characterized in that: in the step 3), the putting amount of the algae segments is 2-2.5 hundred million algae segments/1 m3A body of water.
9. The porphyra haitanensis somatic cell filament seedling transplantation shell seedling collecting method according to claim 1, which is characterized in that: and (5) culturing the shell strings cultured in a hanging mode for a period of time, and turning around for culturing.
10. The porphyra haitanensis somatic cell filament seedling transplantation shell seedling collecting method according to claim 1, which is characterized in that: the shell strings spread on the bottom of the pool are hung up and cultured for a period of time, and then are turned around for culture.
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