CN113945558A - Method for judging myeloproliferation degree - Google Patents
Method for judging myeloproliferation degree Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 23
- 210000001185 bone marrow Anatomy 0.000 claims abstract description 64
- 210000004027 cell Anatomy 0.000 claims abstract description 43
- 210000003743 erythrocyte Anatomy 0.000 claims abstract description 41
- 210000002798 bone marrow cell Anatomy 0.000 claims abstract description 10
- 238000009827 uniform distribution Methods 0.000 claims abstract description 4
- 230000035755 proliferation Effects 0.000 claims description 22
- 230000003247 decreasing effect Effects 0.000 claims description 6
- 238000009432 framing Methods 0.000 claims description 6
- 206010020718 hyperplasia Diseases 0.000 claims description 6
- 230000002390 hyperplastic effect Effects 0.000 claims description 6
- 231100001025 bone marrow hyperplasia Toxicity 0.000 claims description 5
- 230000002071 myeloproliferative effect Effects 0.000 claims description 4
- 208000032839 leukemia Diseases 0.000 description 4
- 208000032467 Aplastic anaemia Diseases 0.000 description 3
- 238000003759 clinical diagnosis Methods 0.000 description 3
- 208000014951 hematologic disease Diseases 0.000 description 3
- 208000007502 anemia Diseases 0.000 description 2
- 230000004663 cell proliferation Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 206010025323 Lymphomas Diseases 0.000 description 1
- 208000034578 Multiple myelomas Diseases 0.000 description 1
- 206010033661 Pancytopenia Diseases 0.000 description 1
- 206010035226 Plasma cell myeloma Diseases 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
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Abstract
The invention relates to a method for judging the myeloproliferation degree, which comprises the following steps: when the bone marrow smear reaches a designated position, starting a gray point camera, and automatically scanning the bone marrow smear by the electronic eyepiece; storing all the pictures of the bone marrow smear collected by the electronic eyepiece for later use; repeatedly collecting a large number of picture samples of the bone marrow smear; dividing the collected picture samples according to the thickness and the uniformity of the bone marrow cells, and generating an algorithm for calculating the thickness and the uniformity of the bone marrow smear according to the divided pictures; collecting a whole picture of the bone marrow smear again; calculating the thickness and the uniformity of the bone marrow smear by using an algorithm, and selecting a region with moderate bone marrow cell thickness and uniform distribution in the bone marrow smear; and respectively calculating the areas of all mature red blood cells and nucleated cells in the frame selection area, calculating the area ratio of the mature red blood cells to the nucleated cells, and judging the myeloproliferation degree by utilizing the area ratio of the red blood cells to the nucleated cells in the bone marrow.
Description
Technical Field
The invention belongs to the field of artificial intelligent medical diagnosis, and particularly relates to a method for judging the myeloproliferation degree.
Background
Myelocytomorphological examination is one of the key diagnostic means in hematopathology, and is commonly used for diagnosing various diseases, including leukemia, multiple myeloma, lymphoma, anemia, pancytopenia and the like, while myeloproliferation degree judgment is an important content in myeloinspection, and has important value for diagnosing diseases with hyperproliferation (such as various leukemias) and hypoproliferation (such as aplastic anemia). At present, the method for judging the myeloproliferation degree mostly adopts a five-level classification method of the institute of hematology of the Chinese medical academy of medicine, and the area with uniform smear thickness is estimated according to the quantitative ratio of nucleated cells and mature red blood cells and is divided into five levels of hyperactive proliferation, obviously active proliferation, reduced proliferation and reduced proliferation severity (see table 1). The method has the advantages that the discrete degree of each grade of the reference interval is large, the critical value is difficult to grasp, and the existing bone marrow smear mostly adopts artificial microscopic examination, and the collection and counting of the number of red blood cells and nucleated cells are manually carried out, so the method is easily influenced by subjective factors.
TABLE 1 bone marrow cell proliferation degree reference interval by five-stage classification of cell number.
Disclosure of Invention
The invention aims to overcome the defects in the prior art and provides a method for judging the myeloproliferation degree, which is simple to operate, is not influenced by subjective factors of people and can greatly improve the accuracy of detecting the myeloproliferation degree.
The technical scheme adopted by the invention for solving the problems is as follows: a method for determining the degree of myeloproliferation is provided, comprising the steps of:
s1, when a bone marrow smear reaches a designated position, starting a gray point camera, and automatically scanning the bone marrow smear by an electronic eyepiece;
s2, storing all the pictures of the bone marrow smear acquired by the electronic eyepiece in the step S1 for later use;
s3, repeating the steps S1-S2, and collecting a large number of picture samples of the bone marrow smear;
s4, dividing the picture samples collected in the step S3 according to the thickness and the uniformity of the bone marrow cells, and generating an algorithm for calculating the thickness and the uniformity of the bone marrow smear according to the divided pictures;
s5, collecting a whole picture of the bone marrow smear again, and calculating the total area of the bone marrow smear;
s6, calculating the thickness and the uniformity of the bone marrow smear by using the algorithm in the step S4, and selecting an area with moderate bone marrow cell thickness and uniform distribution in the bone marrow smear;
s7, respectively calculating the areas of all mature red blood cells and nucleated cells in the boxed area in the step S6, and calculating the area ratio of the mature red blood cells to the nucleated cells;
s8, observing and recording the myeloproliferation degree of the bone marrow smear collected in the step S5 under an electronic eyepiece;
s9, acquiring the area ratio of the mature red blood cells to the nucleated cells in the step S7 corresponding to the bone marrow hyperplasia degree in the step S8;
s10, repeating the steps S5-S9 to obtain the area ratios of mature red blood cells and nucleated cells corresponding to different myeloproliferative degrees;
s11, dividing the area ratio range of mature red blood cells and nucleated cells according to the level of myeloproliferation degree;
s12, collecting a picture of the bone marrow smear, identifying a framing area, calculating the area ratio of mature red blood cells to nucleated cells in the framing area of the bone marrow smear, and determining the myeloproliferation degree of the bone marrow smear according to the area ratio range of the step S11.
Preferably, in the step 11, when the level of proliferation corresponding to the degree of bone marrow proliferation is hyperactive proliferation, the ratio of the area of mature red blood cells to the area of nucleated cells is 1-2.2: 1.
Preferably, in the step 11, when the level of proliferation corresponding to the degree of bone marrow proliferation is significantly active, the ratio of the area of mature red blood cells to the area of nucleated cells is 2.2-5.5: 1.
Preferably, in the step 11, when the level of proliferation corresponding to the degree of bone marrow proliferation is hyperplastic, the ratio of the area of mature red blood cells to the area of nucleated cells is 5.5-21: 1.
Preferably, in the step 11, when the level of proliferation corresponding to the degree of bone marrow proliferation is decreased, the ratio of the area of mature red blood cells to the area of nucleated cells is 21-39: 1.
Preferably, in the step 11, when the level of proliferation corresponding to the degree of bone marrow proliferation is decreased in the degree of proliferation, the ratio of the area of mature erythrocytes to the area of nucleated cells is 39- ∞: 1.
Compared with the prior art, the invention has the following advantages and effects: after the bone marrow smear is scanned by the bone marrow smear scanning system, automatically selecting all areas with uniform thickness on the whole bone marrow smear, calculating the area ratio of red blood cells and nucleated cells in the areas, and determining the bone marrow hyperplasia condition of the bone marrow smear according to the ratio; compared with the traditional method for determining the myeloproliferation degree by utilizing the ratio of the number of the red blood cells to the number of the nucleated cells, the method has more accurate result without the influence of subjective factors, and particularly, special samples cannot be affected by incomplete observation under a microscope.
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FIG. 1 is a flow chart of the method of operation of the present invention.
Detailed Description
The present invention will be described in further detail below by way of examples with reference to the accompanying drawings, which are illustrative of the present invention and are not to be construed as limiting the present invention.
Examples are given.
The invention relates to a method for judging the myeloproliferation degree, which comprises the following steps:
s1, when a bone marrow smear reaches a designated position, starting a gray point camera, and automatically scanning the bone marrow smear by an electronic eyepiece;
s2, storing all the pictures of the bone marrow smear acquired by the electronic eyepiece in the step S1 for later use;
s3, repeating the steps S1-S2, and collecting a large number of picture samples of the bone marrow smear;
s4, dividing the picture samples collected in the step S3 according to the thickness and the uniformity of the bone marrow cells, and generating an algorithm for calculating the thickness and the uniformity of the bone marrow smear according to the divided pictures;
s5, collecting a whole picture of the bone marrow smear again, and calculating the total area of the bone marrow smear;
s6, calculating the thickness and the uniformity of the bone marrow smear by using the algorithm in the step S4, and selecting an area with moderate bone marrow cell thickness and uniform distribution in the bone marrow smear;
s7, respectively calculating the areas of all mature red blood cells and nucleated cells in the boxed area in the step S6, and calculating the area ratio of the mature red blood cells to the nucleated cells;
s8, observing and recording the myeloproliferation degree of the bone marrow smear collected in the step S5 under an electronic eyepiece;
s9, acquiring the area ratio of the mature red blood cells to the nucleated cells in the step S7 corresponding to the bone marrow hyperplasia degree in the step S8;
s10, repeating the steps S5-S9 to obtain the area ratios of mature red blood cells and nucleated cells corresponding to different myeloproliferative degrees;
s11, dividing the area ratio range of mature red blood cells and nucleated cells according to the level of myeloproliferation degree;
s12, collecting a picture of the bone marrow smear, identifying a framing area, calculating the area ratio of mature red blood cells to nucleated cells in the framing area of the bone marrow smear, and determining the myeloproliferation degree of the bone marrow smear according to the area ratio range of the step S11.
As shown in Table 2 below, when the area ratio of mature red blood cells to nucleated cells in the bone marrow smear is 1-2.2:1, the corresponding level of myeloproliferation is hyperplastic, and the results are mainly used for clinical diagnosis of leukemia. When the area ratio of mature red blood cells to nucleated cells in the bone marrow smear is 2.2-5.5:1, the corresponding level of bone marrow hyperplasia is obviously active, and the result is mainly used for clinically diagnosing leukemia and hyperplastic anemia. When the area ratio of mature red blood cells to nucleated cells in the bone marrow smear is 5.5-21:1, the corresponding level of myeloproliferation is hyperplastic, and the result is mainly used for clinically diagnosing normal bone marrow picture and various blood diseases. When the area ratio of mature red blood cells to nucleated cells in a bone marrow smear is 21-39:1, the corresponding level of myeloproliferation is decreased, and the results are mainly used for clinical diagnosis of aplastic anemia and various blood diseases. When the area ratio of mature red blood cells to nucleated cells in a bone marrow smear is 39- ∞:1, the corresponding level of myeloproliferation is decreased in the level of proliferation severity, and this result is mainly used for clinical diagnosis of various hematological diseases such as aplastic anemia and hypoplasia.
Table 2 bone marrow cell proliferation degree according to the five-stage classification of cell area ratio reference interval.
In the method for judging the myeloproliferation degree, the reference visual field is more than the microscopic examination result in the judging process, the reference visual field is obtained by collecting a large amount of image data of a marrow smear, the algorithm is automatically calculated without subjectivity, and the method is more accurate than the original calculating mode according to the number ratio of red blood cells to nucleated cells and is easier to operate.
In addition, it should be noted that the above contents described in the present specification are only illustrations of the present invention. All equivalent or simple changes of the features and principles of the invention according to the patent concepts are included in the scope of protection of the present patent. Various modifications, additions and substitutions for the specific embodiments described may be made by those skilled in the art without departing from the scope of the invention as defined in the accompanying claims.
Claims (6)
1. A method for determining the extent of myeloproliferation, comprising the steps of:
s1, when a bone marrow smear reaches a designated position, starting a gray point camera, and automatically scanning the bone marrow smear by an electronic eyepiece;
s2, storing all the pictures of the bone marrow smear acquired by the electronic eyepiece in the step S1 for later use;
s3, repeating the steps S1-S2, and collecting a large number of picture samples of the bone marrow smear;
s4, dividing the picture samples collected in the step S3 according to the thickness and the uniformity of the bone marrow cells, and generating an algorithm for calculating the thickness and the uniformity of the bone marrow smear according to the divided pictures;
s5, collecting a whole picture of the bone marrow smear again, and calculating the total area of the bone marrow smear;
s6, calculating the thickness and the uniformity of the bone marrow smear by using the algorithm in the step S4, and selecting an area with moderate bone marrow cell thickness and uniform distribution in the bone marrow smear;
s7, respectively calculating the areas of all mature red blood cells and nucleated cells in the boxed area in the step S6, and calculating the area ratio of the mature red blood cells to the nucleated cells;
s8, observing and recording the myeloproliferation degree of the bone marrow smear collected in the step S5 under an electronic eyepiece;
s9, acquiring the area ratio of the mature red blood cells to the nucleated cells in the step S7 corresponding to the bone marrow hyperplasia degree in the step S8;
s10, repeating the steps S5-S9 to obtain the area ratios of mature red blood cells and nucleated cells corresponding to different myeloproliferative degrees;
s11, dividing the area ratio range of mature red blood cells and nucleated cells according to the level of myeloproliferation degree;
s12, collecting a picture of the bone marrow smear, identifying a framing area, calculating the area ratio of mature red blood cells to nucleated cells in the framing area of the bone marrow smear, and determining the myeloproliferation degree of the bone marrow smear according to the area ratio range of the step S11.
2. The method according to claim 1, wherein the ratio of the area of mature red blood cells to the area of nucleated cells is 1-2.2:1 when the level of proliferation corresponding to the degree of myeloproliferation in step 11 is hyperplastic.
3. The method according to claim 1, wherein the ratio of the area of mature red blood cells to the area of nucleated cells is 2.2-5.5:1 when the proliferation level corresponding to the myeloproliferative degree in step 11 is significantly active.
4. The method according to claim 1, wherein the ratio of the area of mature red blood cells to the area of nucleated cells is 5.5-21:1 when the level of proliferation corresponding to the degree of myeloproliferation in step 11 is hyperplastic.
5. The method according to claim 1, wherein the ratio of the area of mature red blood cells to the area of nucleated cells is 21-39:1 when the level of proliferation corresponding to the degree of myeloproliferation in step 11 is decreased.
6. The method according to claim 1, wherein when the level of proliferation corresponding to the degree of myeloproliferation is decreased in the step 11, the ratio of the area of mature erythrocytes to the area of nucleated cells is 39- ∞: 1.
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