CN113943817A - Cervical cancer canceration level evaluation model and construction method - Google Patents
Cervical cancer canceration level evaluation model and construction method Download PDFInfo
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Abstract
Description
ADCYAP1 | CDKN2A | JAM3 | PCDHA4 | TERT |
AJAP1 | DAPK1 | KCNIP4 | POU4F3 | TIMP3 |
ANKRD18CP | DLX1 | LHX8 | RARB | WIF1 |
APC | EPB41L3 | LMX1A | RNASEH2A | ZNF671 |
ASTN1 | FAM19A4 | MAL | RUBCNL | ZSCAN1 |
CADM1 | FANCI | MIR124-1 | RXFP3 | |
CCNA2 | FHIT | MIR124-2 | SLIT2 | |
CDH1 | GATA4 | MIR124-3 | SOX1 | |
CDH13 | GFRA1 | PAX1 | SOX17 | |
CDH6 | HS3ST2 | PCDHA13 | ST6GALNAC5 |
Joint numbering | Linker sequences | Decoration |
1_1 | ACACTCTTTCCCTACACGACGCTCTTCCGATCTCGAGCTCA*T | 5-MethyldC |
1_2 | P-TGAGCTCGAGATCGGAAGAGCACACGTCT | 5-MethyldC |
2_1 | ACACTCTTTCCCTACACGACGCTCTTCCGATCTAGACATGCAG*T | 5-MethyldC |
2_2 | P-CTGCATGTCTAGATCGGAAGAGCACACGTCT | 5-MethyldC |
3_1 | ACACTCTTTCCCTACACGACGCTCTTCCGATCTGTCTAGCAC*T | 5-MethyldC |
3_2 | P-GTGCTAGACAGATCGGAAGAGCACACGTCT | 5-MethyldC |
4_1 | ACACTCTTTCCCTACACGACGCTCTTCCGATCTTACGCTAC*T | 5-MethyldC |
4_2 | P-GTAGCGTAAGATCGGAAGAGCACACGTCT | 5-MethyldC |
5_1 | ACACTCTTTCCCTACACGACGCTCTTCCGATCTCGTCACTAAG*T | 5-MethyldC |
5_2 | P-CTTAGTGACGAGATCGGAAGAGCACACGTCT | 5-MethyldC |
6_1 | ACACTCTTTCCCTACACGACGCTCTTCCGATCTCTCACGTGC*T | 5-MethyldC |
6_2 | P-GCACGTGAGAGATCGGAAGAGCACACGTCT | 5-MethyldC |
7_1 | ACACTCTTTCCCTACACGACGCTCTTCCGATCTATGTCTCA*T | 5-MethyldC |
7_2 | P-TGAGACATAGATCGGAAGAGCACACGTCT | 5-MethyldC |
8_1 | ACACTCTTTCCCTACACGACGCTCTTCCGATCTCACACGTCCA*T | 5-MethyldC |
8_2 | P-TGGACGTGTGAGATCGGAAGAGCACACGTCT | 5-MethyldC |
9_1 | ACACTCTTTCCCTACACGACGCTCTTCCGATCTACATCTCAG*T | 5-MethyldC |
9_2 | P-CTGAGATGTAGATCGGAAGAGCACACGTCT | 5-MethyldC |
10_1 | ACACTCTTTCCCTACACGACGCTCTTCCGATCTCGTAGCGT*T | 5-MethyldC |
10_2 | P-ACGCTACGAGATCGGAAGAGCACACGTCT | 5-MethyldC |
Step (ii) of | Temperature of | Time |
1 | 95℃ | 2min |
2 | Reducing the temperature to 25 ℃ at the speed of 0.1 ℃/8s | About 90min |
3 | 4℃ | Hold |
Prediction/truth | CIN1- | CIN2+ | Total of |
CIN1- | 21 | 2 | 23 |
CIN2+ | 1 | 16 | 17 |
Total of | 22 | 18 | 40 |
Claims (10)
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN115612744A (en) * | 2022-12-14 | 2023-01-17 | 中国医学科学院肿瘤医院 | Human papilloma virus typing and related gene methylation integrated detection model and construction method thereof |
CN115896289A (en) * | 2022-11-24 | 2023-04-04 | 北京迈基诺基因科技股份有限公司 | Detection primer, probe, kit and method for methylation of cervical cancer related genes |
CN117625795A (en) * | 2024-01-25 | 2024-03-01 | 北京迈基诺基因科技股份有限公司 | Probe set, kit and detection system for methylation detection of lung cancer and application |
CN117701718A (en) * | 2024-02-04 | 2024-03-15 | 湖南宏雅基因技术有限公司 | Gene methylation marker for diagnosing cervical cancer, primer pair and application thereof |
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CN105177168A (en) * | 2015-10-27 | 2015-12-23 | 山东大学齐鲁医院 | Reagent kit for screening early-stage cervical cancers based on cervical liquid-based cytology and DNA methylation |
CN108103193A (en) * | 2017-12-18 | 2018-06-01 | 东莞博奥木华基因科技有限公司 | Methylation detecting method based on cervical carcinoma host cell |
CA3121886A1 (en) * | 2018-12-04 | 2020-06-11 | Hkg Epitherapeutics Limited | Dna methylation biomarkers for early detection of cervical cancer |
CN111635940A (en) * | 2020-06-03 | 2020-09-08 | 王思贤 | Kit and pharmaceutical composition for cervical cancer detection, screening, typing, diagnosis or prognosis evaluation |
CN112375822A (en) * | 2020-06-01 | 2021-02-19 | 广州市基准医疗有限责任公司 | Methylation biomarker for detecting breast cancer and application thereof |
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2021
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Patent Citations (6)
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CN105177168A (en) * | 2015-10-27 | 2015-12-23 | 山东大学齐鲁医院 | Reagent kit for screening early-stage cervical cancers based on cervical liquid-based cytology and DNA methylation |
CN108103193A (en) * | 2017-12-18 | 2018-06-01 | 东莞博奥木华基因科技有限公司 | Methylation detecting method based on cervical carcinoma host cell |
CA3121886A1 (en) * | 2018-12-04 | 2020-06-11 | Hkg Epitherapeutics Limited | Dna methylation biomarkers for early detection of cervical cancer |
WO2020115728A2 (en) * | 2018-12-04 | 2020-06-11 | Hkg Epitherapeutics Limited | Dna methylation biomarkers for early detection of cervical cancer |
CN112375822A (en) * | 2020-06-01 | 2021-02-19 | 广州市基准医疗有限责任公司 | Methylation biomarker for detecting breast cancer and application thereof |
CN111635940A (en) * | 2020-06-03 | 2020-09-08 | 王思贤 | Kit and pharmaceutical composition for cervical cancer detection, screening, typing, diagnosis or prognosis evaluation |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
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CN115896289A (en) * | 2022-11-24 | 2023-04-04 | 北京迈基诺基因科技股份有限公司 | Detection primer, probe, kit and method for methylation of cervical cancer related genes |
CN115612744A (en) * | 2022-12-14 | 2023-01-17 | 中国医学科学院肿瘤医院 | Human papilloma virus typing and related gene methylation integrated detection model and construction method thereof |
CN117625795A (en) * | 2024-01-25 | 2024-03-01 | 北京迈基诺基因科技股份有限公司 | Probe set, kit and detection system for methylation detection of lung cancer and application |
CN117701718A (en) * | 2024-02-04 | 2024-03-15 | 湖南宏雅基因技术有限公司 | Gene methylation marker for diagnosing cervical cancer, primer pair and application thereof |
CN117701718B (en) * | 2024-02-04 | 2024-05-07 | 湖南宏雅基因技术有限公司 | Gene methylation marker for diagnosing cervical cancer, primer pair and application thereof |
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Inventor after: Chen Men Inventor after: Wu Jian Inventor after: Chen Tingting Inventor after: Wang Yakun Inventor after: Yin Jian Inventor after: Ji Xiaowen Inventor after: Wang Haili Inventor after: Liu Na Inventor after: Han Lu Inventor before: Wu Jian Inventor before: Ji Xiaowen Inventor before: Wang Haili Inventor before: Liu Na Inventor before: Han Lu |
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Effective date of registration: 20230718 Address after: 100021 No. 17, South Lane, Chaoyang District, Beijing, Panjiayuan Patentee after: CANCER HOSPITAL, CHINESE ACEDEMY OF MEDICAL SCIENCES Patentee after: BEIJING MYGENOSTICS CO.,LTD. Address before: 101312 201, block a, building 12, courtyard 9, Anqing street, airport economic core area, Shunyi District, Beijing Patentee before: BEIJING MYGENOSTICS CO.,LTD. |