CN113943680A

CN113943680A - Microbacterium marinum and application thereof in degrading T-2 toxin

Info

Publication number: CN113943680A
Application number: CN202111319820.3A
Authority: CN
Inventors: 赵晨; 宋佳; 孙博
Original assignee: Academy of National Food and Strategic Reserves Administration
Current assignee: Academy of National Food and Strategic Reserves Administration
Priority date: 2021-11-09
Filing date: 2021-11-09
Publication date: 2022-01-18

Abstract

The invention discloses a Microbacterium marinum and application thereof in degrading T-2 toxin. The Microbacterium maritima (Microbacterium maritima) TD-1 has a preservation number of CGMCC No. 23143. The invention also provides a microbial inoculum containing the preserved strain and a preparation method thereof. In addition, the invention also provides application of the strain in degrading T-2 toxin. The strain can completely degrade T-2 toxin in a short time.

Description

Microbacterium marinum and application thereof in degrading T-2 toxin

Technical Field

The invention belongs to the field of microbial application, and particularly relates to a Microbacterium maritima strain (Microbacterium maritima) and application thereof in degradation of T-2 toxin.

Background

The T-2 toxin is a sesquiterpene compound with the chemical name of 4 beta-1, 5-diacetoxy-8 alpha- (3-methyl butyryloxy) -3 alpha-carboxyl-12, 13-epoxy trichothecene-9-ene and the molecular formula of C₂₄H₃₄O₉. It is a trichothecene mycotoxin of A class produced by Fusarium (such as Fusarium sporotrichioides, Fusarium trilobatum, etc.), and has the functions of inhibiting the growth of trichothecenes and promoting the growth of trichothecenesHas the characteristics of wide distribution, quick propagation, heat resistance, high toxicity, long residual time, difficult treatment and the like. The T-2 toxin can be transmitted through a food chain and accumulated in organisms, and further poses a great threat to the safety of animal bodies, animal-derived food such as meat, poultry, eggs and milk, and human health. The main target organ for its action is the tissue organ with vigorous cell division. The acute toxicological characteristics of the toxin are mainly manifested by vomit, chest pain, dizziness, heartbeat retardation, diarrhea, bleeding and the like; chronic toxicity is mainly manifested by decreased utilization of food or feed, skin necrosis, gastrointestinal mucosal injury, hematopoietic system destruction and immune system suppression, decreased blood coagulation function, nervous system disorder, dysfunction of cardiovascular system, etc.

The T-2 toxin is mainly distributed in mildewed corn, rye, wheat, rice and other grain crops and products thereof, and feed. Samples are collected from feed factories, warehouses and customers in south China, North China and China for toxin detection, and the detection rate of T-2 toxin in the corn sample is 87.5%; the detection rate of T-2 toxin in the protein feed is 97.8 percent. T-2 toxin is relatively easily produced on starch-rich cereal seeds, which are likely to be contaminated by T-2 toxin in the fields, during the harvesting process, during post-harvest storage, and in many links such as storage and use of feed and food products. The grain polluted by the toxin not only threatens and harms the health of people and livestock, but also severely restricts the export trade of agricultural products in China.

Therefore, how to reduce or completely remove mycotoxins has become a hot spot of research at home and abroad. At present, the detoxification method of T-2 toxin mainly comprises a physical method, a chemical method and a biological method. T-2 toxin has stable property and strong heat resistance and ultraviolet tolerance, so the traditional physics such as heating, irradiation, adsorption treatment and the like can partially remove mycotoxin, but the methods have the defects of incomplete detoxification, high cost and the like. The effect of removing mycotoxin by chemical detoxification is better than that of physical detoxification, but chemical detoxification may influence the safety and palatability of the feed, and practical application has certain limitations. The biological method has mild detoxification conditions, high efficiency and specificity, can furthest reserve the nutrient components in the grains, and has no pollution to the environment by degraded products, so the biological method is increasingly concerned by the scientific community. Some of the microorganisms that have been selected to remove T-2 toxins, such as yeast, Bacillus, atypical Arthrobacter, etc., have been screened. However, the development and research of T-2 toxin-free microorganisms at home and abroad are still insufficient at present, the T-2 toxin-free efficiency of partial strains is low, after analysis of metabolites, a part of microorganisms convert the T-2 toxin into HT-2 toxin or T-2 triol and the like, and the toxicity of the products is lower than that of the T-2 toxin or stronger than that of the T-2 toxin. Therefore, the development of the microbial strains which can degrade the T-2 toxin efficiently, safely and specifically has important significance.

Disclosure of Invention

The first purpose of the invention is to provide a Microbacterium marinum strain which can efficiently degrade T-2 toxin.

The second purpose of the invention is to provide a microbial inoculum containing the bacterial strain and a preparation method thereof.

The third purpose of the invention is to provide the application of the strain in degrading T-2 toxin.

In order to solve the technical problems, the invention adopts the following technical scheme:

in a first aspect, the invention provides a Microbacterium marinum (Microbacterium maritima) with a bacterial name TD-1 and a classification name: the Haizhijie Microbacterium maritypicum has been deposited in China general microbiological culture Collection center (CGMCC) at 13.08.2021 (address: Beijing, West Lu No.1, Ministry of microbiology, China academy of sciences, Japan) with the collection number of CGMCC No. 23143.

Specifically, the physicochemical properties of the microbacterium marinum are as follows:

1. morphological characteristics of the cells:

the color of the strain is light, the bacterial colonies are round and very small, the bacterial colonies are not easy to separate from each other, the edges are smooth, the strain is glossy, and the strain is semitransparent for light. The strain has irregular rod shape under optical microscope, and has size of (0.3-0.6) × (1.2-2.9) μm (FIG. 6 and FIG. 7).

2. The main physicochemical characteristics of the strain are as follows:

the Microbacterium maritypium TD-1 is suitable for growth at 25-30 deg.C and pH 6-9, and has aerobic growth and no spore formation.

3. The main genetic characteristics of the strains

Sequencing 16SrDNA of the strain, and BLAST comparison shows that the sequence of the 16SrDNA gene of the strain has higher similarity with the existing strain of Microbacterium maritypicum, wherein the sequence similarity with the Helicobacter is 99.86%.

By combining the physiological and biochemical characteristics and the sequence comparison result of 16SrDNA, the Microbacterium maritypticum strain belongs to the genus Microbacterium, and is named Microbacterium maritypticum TD-1(T-2degradation strain 1) because the Microbacterium maritypticum strain can degrade T-2 toxin.

As in the case of other organisms, the strain TD-1, which is a bacterium having the activity of degrading T-2 toxin according to the present invention, may be mutated or mutated. Thus, mutants of the strain may be obtained by physical and chemical methods known in the art, for example, by using chemical agents such as Nitrosoguanidine (NTG) and other chemical mutagens, or by physical methods such as UV, Co₆₀Irradiation to obtain mutants, which are also part of the present invention, as long as the ability to degrade T-2 toxin is retained.

In a second aspect, the invention also provides a microbial inoculum, which contains the Microbacterium maritima (Microbacterium maritima) TD-1. The microbial inoculum can be in a liquid dosage form or a solid dosage form, and can be prepared by a preparation method disclosed in the prior art. Specifically, the invention provides a preparation method of the microbial inoculum, which comprises the following steps:

activating Microbacterium maritima (Microbacterium maritima) with the preservation number of CGMCC No.23143, performing multi-stage propagation, and collecting fermentation liquid when the thallus is in a stationary phase to prepare a liquid microbial inoculum.

Further, the method may further comprise a step of preparing the liquid type microbial inoculum into a solid type microbial inoculum. Optionally, mixing the liquid microbial inoculum with an adsorbent, and drying to obtain a solid microbial inoculum; or spray drying the liquid microbial inoculum to obtain the solid microbial inoculum. The liquid microbial inoculum is mixed with an adsorbent according to the weight ratio of 1:2-10, wherein the adsorbent is one or more of corncob powder, bran, starch, diatomite, attapulgite, vermiculite, light calcium carbonate or peat.

Further, in the above-mentioned preparation method, the fermentation medium (OP) for culturing Microbacterium marinum may take various forms, but in view of the production cost, cell biomass, detoxification activity, etc., it is preferable that some medium is used, for example, a preferable carbon source of Microbacterium marinum TD-1 is sucrose, but glucose, maltose, glycerol, fructose, galactose, mannose, starch, etc. may be used. A preferred nitrogen source for Microbacterium maritima TD-1 is soy peptone, but tryptone, yeast extract, beef extract, urea, and the like may also be used. Among the nutrient inorganic salts which can be incorporated into the culture medium are the conventional soluble salts which are capable of generating the following ions: sodium ion, potassium ion, magnesium ion, calcium ion, iron ion, chloride ion, phosphate ion, sulfate ion, and the like.

Further, the seed culture medium and the fermentation culture medium (OP) need to be cooled to 30-35 ℃ after being sterilized by high-temperature moist heat at 121 ℃.

Further, inoculating the activated strain into a seed tank according to the inoculation amount of 0.5-10% of a seed culture medium in the seed tank; and inoculating the seed solution into a fermentation tank according to the inoculation amount of 0.5-10% of the fermentation medium in the fermentation tank for expanding culture.

Further, the fermentation culture conditions are as follows: the ventilation capacity of sterile air is 1: 0.5-1.2, the stirring speed is 50-300 r/min, the culture temperature is about 30-37 ℃, and the number of living cells in the liquid microbial inoculum at least reaches 10⁷CFU/mL。

In a third aspect, the invention also provides an application of the Microbacterium maritima (Microbacterium maritima) TD-1 with the preservation number of CGMCC No.23143, which is at least one of the following 1) or 2):

1) degrading T-2 toxin;

2) preparing a product for degrading T-2 toxin.

According to a specific embodiment of the present invention, the method for degrading T-2 toxin of the present invention is to treat a material containing T-2 toxin with Microbacterium maritima (TD-1) as described above.

Further, any of the following (a) or (b) modes may be adopted:

(a) spraying a liquid microbial inoculum to T-2 toxin-polluted grains, feeds, feed raw materials or other agricultural and sideline products according to the mass ratio of 1:1 to degrade the T-2 toxin, wherein the number of living cells of the liquid microbial inoculum at least reaches 10⁷CFU/mL。

(b) Adding a solid microbial inoculum into grains or feed and feed raw materials according to the addition amount of 1-5% by mass, uniformly mixing, and degrading T-2 toxin, wherein the number of living cells of the solid microbial inoculum at least reaches 10⁸CFU/g。

In some embodiments, the grain is corn, barley, rice, wheat, or sorghum. The feed raw materials and the feed are corn, barley, rice, wheat or sorghum and the like and the feed processed by the corn, the barley, the rice, the wheat or the sorghum. The other agricultural and sideline products are byproducts of agricultural product processing such as vinasse, pomace, starch residue and the like.

Compared with the prior art, the invention has the following advantages and effects:

the Microbacterium maritima (Microbacterium maritima) TD-1 strain with the preservation number of CGMCC No.23143 has efficient degradation effect on T-2 toxin, can completely degrade 15 microgram/mL of T-2 toxin within 20h, and has the advantages of high degradation speed and complete degradation. The bacterial agent for degrading the T-2 toxin produced by the strain with the preservation number of CGMCC No.23143 has the advantages of low production and use cost, simplicity, easy operation, safe degradation, high efficiency and the like. The strain and the microbial inoculum provided by the invention can be used for removing T-2 toxin in grains, feeds and feed raw materials, and have important significance for solving the problem of toxin pollution in the grains, the feeds and the raw materials thereof, improving the utilization rate of grains, ensuring the safe production of animal husbandry and improving the economic benefit of the animal husbandry.

Deposit description

Reference biological material (strain): TD-1

Suggested classification nomenclature: microbacterium maritypicum

The preservation organization: china general microbiological culture Collection center

The preservation organization is abbreviated as: CGMCC (China general microbiological culture Collection center)

Address: xilu No.1 Hospital No. 3 of Beijing market facing Yang district

The preservation date is as follows: 2021 year 08 month 13 day

Registration number of the preservation center: CGMCC No. 23143.

Drawings

FIG. 1 the effect of TD-1 on the degradation of toxins in a medium with a T-2 toxin concentration of 15. mu.g/mL.

FIG. 2 shows the degradation effect of the liquid reaction system TD-1.

FIG. 3 shows the degradation effect of the TD-1 liquid microbial inoculum on T-2 toxin in grains.

FIG. 4 shows the degradation effect of the solid microbial inoculum TD-1 on T-2 toxin in grains.

FIG. 5 is a comparison of the effect of TD-1 on T-2 toxin degradation in fermentation medium before and after optimization.

FIG. 6 is a colony morphology map of TD-1.

FIG. 7 is a microphotograph of TD-1.

Detailed Description

The present invention is further illustrated by the following examples, but is not limited thereto. The experimental procedures in the following examples are conventional unless otherwise specified. The test materials used in the following examples were purchased from conventional biochemical suppliers, unless otherwise specified.

Example 1 Microbacterium maritima

231 parts of soil samples are collected from areas seriously polluted by Jiangzhe mildew, a 96-microporous plate is used as a culture carrier, a T-2 toxin degradation bacterial suspension is obtained by firstly adopting an enrichment culture method of T-2 toxin concentration gradient for 5 times continuously, then the bacterial suspension is coated on an LB agar plate (yeast extract 0.5%, tryptone 1%, NaCl 1%, agar powder 1.6%, pH7.2, sterilization is carried out at 121 ℃ for 20min) according to a dilution coating method with proper dilution, colonies with good growth and separation degree, colony morphology characteristics and different colors are selected, a detoxification test is carried out in an LB liquid culture medium (yeast extract 0.5%, tryptone 1%, NaCl 1%, pH7.2, sterilization is carried out at 121 ℃ for 20min) with the T-2 toxin concentration of 15 mu g/mL, acetonitrile is extracted, residual T-2 toxin is detected, the T-2 toxin degradation effect of each pure culture is verified, finally successfully obtaining a strain TD-1 capable of degrading T-2 toxin, selecting a single TD-1 colony in an LB liquid culture medium, culturing to the middle logarithmic phase, mixing a 30% glycerol solution (glycerol: water: 1) and the culture in equal volume, and storing at-80 ℃.

Cloning the 16S rDNA sequence of the strain TD-1, sequencing the 16S rDNA sequence (see SEQ ID No.1 of the sequence table), and carrying out BLAST comparison on the sequencing result on Genbank and EZBioClaud websites to analyze sequence homology so as to obtain the phylogenetic position identification result of the strain TD-1. Finally, the Microbacterium maritima TD-1 is delivered to China general microbiological culture collection center for collection with the collection number of CGMCC No. 23143. For simplicity of description, in the following description, Microbacterium maritima (Microbacterium maritima) TD-1 referred to in the present invention is denoted by TD-1.

EXAMPLE 2 detoxification Effect accompanying TD-1 fermentation

Inoculating activated TD-1 into 50mL culture medium containing 15 mug/mL T-2 toxin in an inoculation amount of 1%, performing detoxification culture under the condition of oscillation at 200 rpm, sampling at regular time and extracting T-2 toxin by acetonitrile, wherein the residual content of the T-2 toxin in the final culture is shown by HPLC detection results: as the fermentation proceeded, the T-2 toxin was completely degraded, and after 20 hours, the T-2 toxin degradation rate reached 100% (see FIG. 1). And a graph (degradation curve) of the degradation effect of the above strain in a liquid reaction system was plotted (see FIG. 2).

EXAMPLE 3 preparation of TD-1 liquid inoculum

The solid culture medium comprises the following components in percentage by weight: 0.5% of yeast extract, 1% of tryptone, 1% of NaCl, 1.6% of agar powder, 7.2-7.4% of pH, and sterilizing for 20min by high-temperature steam at 121 ℃.

The seed culture medium comprises the following components in percentage by weight: 0.5% of yeast extract, 1% of tryptone, 1% of NaCl, 7.2-7.4 of pHs, and sterilizing for 20min by high-temperature steam at 121 ℃; the fermentation medium comprises the following components in percentage by weight: TD-1: yeast extract 0.5%, tryptone 1%, sodium chloride 1%, pH 7.2.

Activating strains: TD-1 with the preservation number of CGMCC No.23143 is inoculated on a solid culture medium, cultured for 2 days at the temperature of 30 ℃, the degradation performance of the T-2 toxin is measured, and then the TD-1 is inoculated on a test tube inclined plane for standby.

Seed culture: picking single colony from a slant culture medium, inoculating the single colony into a seed culture medium, and culturing at 30 ℃ until logarithmic phase to obtain a spare strain; then, a 100-liter seeding tank is used, the feeding amount of a seed culture medium is 70 liters, after the feeding is finished, the high-pressure moist heat sterilization is carried out at the temperature of 121 ℃, after the cooling is carried out to the temperature of 30 ℃, the cultured strains are inoculated into the seeding tank according to the inoculation amount of 2 percent of the volume ratio, the stirring speed is 220 r/min, the culture temperature is 30 ℃, the introduction amount of sterile air is 1:1 (volume ratio), and the strains are cultured for about 24 to 40 hours until the logarithmic phase to obtain a seed solution.

Fermentation culture: adopting a production tank with the volume of 1000 liters, and the feeding amount of a fermentation medium is 600 liters and is 1.1kg/cm²Performing high-pressure moist heat sterilization at the temperature of 121 ℃, cooling to 30 ℃ after sterilization, inoculating the seed liquid into a fermentation tank according to the inoculation amount of 2%, and performing fermentation conditions as follows: the ventilation capacity of the sterile air is 1: 1-1.2, the stirring speed is 200-260 r/min, the culture temperature is 30 ℃, the culture time is about 24-40 hours, and a liquid microbial inoculum with the T-2 toxin degradation capability is formed after the culture tank is placed. The number of living cells in the liquid microbial inoculum reaches at least 10⁷CFU/ml。

EXAMPLE 4 preparation of TD-1 solid microbial inoculum

The TD-1 solid microbial inoculum produced in example 3 is uniformly mixed with wheat bran or corncob powder according to a mass ratio of 1:5, dried at a low temperature of below 40 ℃ until the water content is below 10% to form a solid state, ground into powder, and subpackaged for storage, so that the TD-1 solid microbial inoculum is prepared.

Example 5 degradation Effect of TD-1 liquid microbial inoculum on T-2 toxin in cereals

The TD-1 liquid bacterium prepared in example 3 was usedDiluting with the agent to obtain diluted liquid strain with viable cell number at least 10⁷Each volume/mL is sprayed into wheat flour contaminated with T-2 toxin according to the mass ratio of 1:1 to serve as a test group, a sterile fermentation medium is sprayed into the wheat flour contaminated with T-2 toxin according to the same mass ratio to serve as a control group, each group is repeated three times, after being uniformly mixed, detoxification is carried out for 48 hours at the temperature of 30 ℃, and then 5g of samples are accurately weighed from the control group and the test group. T-2 toxin residues were analyzed using T-2 toxin enzyme-linked immunoassay kit manufactured by ROMER. The result shows that the degradation rate of the TD-1 liquid microbial inoculum to the T-2 toxin-polluted corn flour reaches 49.9 percent, while the control group has no degradation phenomenon (the result is shown in figure 3).

Example 6 degradation Effect of TD-1 solid microbial inoculum on T-2 toxin in cereals

The TD-1 solid microbial inoculum prepared in example 4 was added to wheat flour contaminated with T-2 toxin in a weight ratio of 1%, 3% and 5%, a mixture of a fermentation medium and wheat bran (mixed in a ratio of 1: 5) was added to wheat flour contaminated with T-2 toxin in a weight ratio of 1%, 3% and 5%, deionized water was added to each of the control group and the test group in a ratio of 1:1, each group was repeated three times, after mixing uniformly, detoxification was carried out at a temperature of 30 ℃ for 48 hours, and 5g of samples were accurately weighed from the control group and the test group. T-2 toxin residue is analyzed by using a T-2 toxin enzyme-linked immunoassay kit produced by ROMER company, and the result shows that the TD-1 solid microbial inoculum detoxifies polluted wheat flour for 48 hours, the degradation rate can reach 31 percent (when the addition rate is 5 percent), and a control group has no degradation phenomenon (the result is shown in figure 4).

Example 7TD-1 degradation T-2 toxin alignment before and after optimization of liquid Medium

Based on the fermentation medium in example 3, the effects of different carbon sources, nitrogen sources, inorganic salts and different pH on TD-1 fermentation were investigated. Determining the components and contents of the optimized culture medium through an orthogonal experiment: yeast extract 0.5%, tryptone 1%, sodium chloride 1%, sucrose 2%, soy peptone 0.5%, dipotassium hydrogen phosphate 5mM, pH 7.0. By using the method in the embodiment 2, TD-1 is respectively inoculated to the original fermentation medium and the optimized fermentation medium, 15 mu g/mL of T-2 toxin is added, the culture conditions are the same as those in the embodiment 2, sampling is carried out at different times in the culture process, the content of the T-2 toxin is detected by liquid phase after acetonitrile extraction, and the degradation efficiency of the TD-1 on the T-2 toxin in different fermentation media is compared. As shown in FIG. 5, the optimized fermentation medium of TD-1 can degrade T-2 toxin faster than the medium before optimization, and the complete degradation time is advanced to 8 h.

It should be understood that the above-described embodiments of the present invention are merely examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. Not all embodiments are exhaustive. All obvious changes and modifications which are obvious to the technical scheme of the invention are covered by the protection scope of the invention.

SEQUENCE LISTING

<110> national institute of food and Material Reserve science

<120> Microbacterium maritima and application thereof in degradation of T-2 toxin

<130> JLP21I0439

<160> 1

<170> PatentIn version 3.5

<210> 1

<211> 1434

<212> DNA

<213> 16S rDNA

<400> 1

tttgagtttg atcctggctc aggatgaacg ctggcggcgt gcttaacaca tgcaagtcga 60

acggtgaaca cggagcttgc tctgtgggat cagtggcgaa cgggtgagta acacgtgagc 120

aacctgcccc tgactctggg ataagcgctg gaaacggcgt ctaatactgg atatgtgacg 180

tgaccgcatg gtctgcgtct ggaaagaatt tcggttgggg atgggctcgc ggcctatcag 240

cttgttggtg aggtaatggc tcaccaaggc gtcgacgggt agccggcctg agagggtgac 300

cggccacact gggactgaga cacggcccag actcctacgg gaggcagcag tggggaatat 360

tgcacaatgg gcgcaagcct gatgcagcaa cgccgcgtga gggacgacgg ccttcgggtt 420

gtaaacctct tttagcaggg aagaagcgaa agtgacggta cctgcagaaa aagcgccggc 480

taactacgtg ccagcagccg cggtaatacg tagggcgcaa gcgttatccg gaattattgg 540

gcgtaaagag ctcgtaggcg gtttgtcgcg tctgctgtga aatccggagg ctcaacctcc 600

ggcctgcagt gggtacgggc agactagagt gcggtagggg agattggaat tcctggtgta 660

gcggtggaat gcgcagatat caggaggaac accgatggcg aaggcagatc tctgggccgt 720

aactgacgct gaggagcgaa agggtgggga gcaaacaggc ttagataccc tggtagtcca 780

ccccgtaaac gttgggaact agttgtgggg tccattccac ggattccgtg acgcagctaa 840

cgcattaagt tccccgcctg gggagtacgg ccgcaaggct aaaactcaaa ggaattgacg 900

gggacccgca caagcggcgg agcatgcgga ttaattcgat gcaacgcgaa gaaccttacc 960

aaggcttgac atatacgaga acgggccaga aatggtcaac tctttggaca ctcgtaaaca 1020

ggtggtgcat ggtgtcctca gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc 1080

gcaaccctcg ttctatgttg ccagcacgta atggtgggaa ctcatgggat actgccgggg 1140

tcaactcgga ggaaggtggg gatgacgtca aatcatcatg ccccttatgt cttgggcttc 1200

acgcatgcta caatggccgg tacaaagggc tgcaataccg cgaggtggag cgaatcccaa 1260

aaagccggtc ccagttcgga ttgaggtctg caactcgacc tcatgaagtc ggagtcgcta 1320

gtaatcgcag atcagcaacg ctgcggtgaa tacgttcccg ggtcttgtac acaccgcccg 1380

tcaagtcatg aaagtcggta acacctgaag ccggtggcct aacccttgtg gagg 1434

Claims

1. Microbacterium maritima (Microbacterium maritima) TD-1 with the preservation number of CGMCC No. 23143.

2. A microbial agent comprising Microbacterium maritima (TD-1) according to claim 1.

3. The microbial inoculum according to claim 2, which is a liquid microbial inoculum or a solid microbial inoculum.

4. A method for producing the microbial agent according to claim 2, which comprises: activating Microbacterium maritima (Microbacterium maritima) TD-1 with the preservation number of CGMCC No.23143, performing multi-stage propagation, and collecting fermentation liquid to obtain liquid microbial inoculum.

5. The method according to claim 4, further comprising a step of preparing the liquid type microbial inoculum into a solid type microbial inoculum.

6. The preparation method according to claim 5, wherein the liquid microbial inoculum is mixed with an adsorbent and dried to obtain a solid microbial inoculum; or spray drying the liquid microbial inoculum to obtain the solid microbial inoculum.

7. The preparation method of claim 6, wherein the liquid microbial inoculum is mixed with an adsorbent in a weight ratio of 1:2-10, wherein the adsorbent is one or more of corncob meal, bran, starch, diatomite, attapulgite, vermiculite, light calcium carbonate or peat.

8. The use of Microbacterium maritima (Microbacterium maritima) TD-1 according to claim 1, which is at least one of 1) or 2) as follows:

1) degrading T-2 toxin;

2) preparing a product for degrading T-2 toxin.

9. A method for degrading T-2 toxin is characterized in that a substance containing the T-2 toxin is treated by Microbacterium maritima (Microbacterium maritima) TD-1 with the preservation number of CGMCC No. 23143.

10. The method according to claim 9, wherein the T-2 toxin-containing substance is treated by either one of the following means (a) or (b):