CN113942154B - Bionic leaf model and preparation method and application thereof - Google Patents
Bionic leaf model and preparation method and application thereof Download PDFInfo
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- CN113942154B CN113942154B CN202111135975.1A CN202111135975A CN113942154B CN 113942154 B CN113942154 B CN 113942154B CN 202111135975 A CN202111135975 A CN 202111135975A CN 113942154 B CN113942154 B CN 113942154B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C39/00—Shaping by casting, i.e. introducing the moulding material into a mould or between confining surfaces without significant moulding pressure; Apparatus therefor
- B29C39/02—Shaping by casting, i.e. introducing the moulding material into a mould or between confining surfaces without significant moulding pressure; Apparatus therefor for making articles of definite length, i.e. discrete articles
- B29C39/021—Shaping by casting, i.e. introducing the moulding material into a mould or between confining surfaces without significant moulding pressure; Apparatus therefor for making articles of definite length, i.e. discrete articles by casting in several steps
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C39/00—Shaping by casting, i.e. introducing the moulding material into a mould or between confining surfaces without significant moulding pressure; Apparatus therefor
- B29C39/22—Component parts, details or accessories; Auxiliary operations
- B29C39/26—Moulds or cores
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C39/00—Shaping by casting, i.e. introducing the moulding material into a mould or between confining surfaces without significant moulding pressure; Apparatus therefor
- B29C39/22—Component parts, details or accessories; Auxiliary operations
- B29C39/38—Heating or cooling
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention belongs to the technical field of manufacturing of microfluidic chips. The invention provides a bionic leaf model and a preparation method and application thereof, wherein the preparation method of the model comprises the following steps: placing fresh leaves in ethanol solution for sterilization, wherein the back surfaces of the leaves are used as male molds; adhering double-sided adhesive tape to a glass plate, adhering the front surface of the leaf to the double-sided adhesive tape, covering a layer of PDMS polymer on the surface of a male mold, vacuumizing to remove bubbles, and then performing curing treatment to obtain a female mold of the bionic leaf model; placing the modified liquid in a container, suspending the female die of the bionic leaf model on the upper side of the modified liquid, sealing the container, standing to evaporate the modified liquid, and forming an isolation layer on the surface of the female die of the bionic leaf model; pouring the PDMS polymer onto the modified bionic leaf model female die, vacuumizing to remove bubbles, and then performing curing treatment to obtain the bionic leaf model. The bionic leaf model can get rid of season limitation, and is convenient for experiments; can be repeatedly used, and protects the environment; and controlling the variable.
Description
Technical Field
The invention relates to the field of manufacturing of microfluidic chips, in particular to a bionic leaf model and a preparation method and application thereof.
Background
The pulse sequence of the fresh leaves is complicated, and the microfluidic chip manufactured by using the pulse sequence can simulate and observe the component flow condition in the blood vessel in the human body, and can be used for medical research.
However, general microfluidic chip fabrication has certain limitations: 1) As the back vein sequence of the leaves is used as the male mould, the leaves have season limitation, so that the manufacture of chips is influenced, and the follow-up experiment is influenced.
2) The frequent picking of leaves can cause a certain damage to the environment.
3) The template can be used for manufacturing a unified micro-fluidic chip, and the shape used in the subsequent experiments is consistent, so that variables are controlled.
4) The height, width and length of each structural channel of the microfluidic chip manufactured by the template are not unique fixed values, and the vascular structure in a human body can be better simulated.
In order to solve the problems, the invention provides a bionic leaf model for manufacturing a microfluidic chip without being limited by seasons. At present, reports related to a bionic leaf model are not yet found.
Disclosure of Invention
In view of the above, the first object of the present invention is to provide a method for preparing a bionic leaf model of a microfluidic chip, which is not limited by seasons, and the method has the advantages of simple preparation process, low cost and suitability for popularization and application.
In order to achieve the above purpose, the invention adopts the following technical scheme:
the preparation method of the bionic leaf model comprises the following steps:
s1, fresh leaves are treated
Placing the picked fresh leaves into an ethanol solution for disinfection treatment, and then wiping the leaves with filter paper;
s2, preparation of bionic leaf model female die
Taking the back of the leaf as a male die, adhering double-sided adhesive tapes on a glass plate, adhering the front of the leaf on the double-sided adhesive tapes, covering a layer of PDMS polymer on the surface of the male die, vacuumizing to remove bubbles, and then performing curing treatment to obtain a bionic leaf model female die;
s3, female die modification of bionic leaf model
Placing the modified liquid at the bottom of the container, suspending the bionic leaf model female die prepared in the step S2 at the upper side of the modified liquid, sealing the container, standing to evaporate the modified liquid, and forming an isolation layer on the surface of the bionic leaf model female die;
s4, preparing a bionic leaf model finished product
Pouring the PDMS polymer onto a female die of the bionic leaf model modified by the S3, vacuumizing to remove bubbles, and then performing curing treatment to obtain the bionic leaf model with a pulse sequence structure.
Preferably, in S3, the modifying liquid is trichloro (1 h,2 h-perfluorooctyl) silane.
Preferably, in S1, the volume concentration of the ethanol solution is 70% -80%; further preferably, the ethanol solution has a volume concentration of 75%.
Preferably, in S2, the curing process is as follows: solidifying for 40-45 min at 75-80 ℃; further preferably, the curing is carried out at 80℃for 45min.
Preferably, in S4, the curing process is as follows: solidifying for 11-12 h at 75-80 ℃; it is further preferred that the curing is carried out at 80℃for 12h.
The second object of the invention is to provide a bionic leaf model which can be used for manufacturing a microfluidic chip without being limited by seasons. The bionic leaf model can get rid of season limitation, and is convenient for experiments; can be repeatedly used, and protects the environment; and controlling the variable.
In order to achieve the above purpose, the invention adopts the following technical scheme: the bionic leaf model prepared by the preparation method comprises a model substrate and a pulse sequence model, wherein the pulse sequence model is arranged on the model substrate, and the model substrate is in integral adsorption connection with the pulse sequence model.
Preferably, the arrangement of the pulse sequence model is consistent with the pulse sequence of the picked fresh tree leaves.
Preferably, the heights of the different vein sequence models are consistent with the heights of the picked fresh tree leaves.
The third object of the invention is to provide an application of the bionic leaf model in manufacturing a microfluidic chip.
The specific operation is as follows:
step one, placing bionic leaves on a glass plate, covering a layer of PDMS polymer on the surface of a male die, vacuumizing to remove bubbles, and then performing curing treatment to obtain a microfluidic channel;
and step two, bonding the microfluidic channel with a glass plate to obtain the microfluidic chip.
The bionic leaf model can be used for manufacturing microfluidic chips without being limited by seasons, and can effectively solve the problems in the background technology.
Compared with the prior art, the invention has the following beneficial effects:
1. the bionic leaf model comprises a model substrate and a pulse sequence, wherein the model substrate can effectively protect the pulse sequence model and prevent impurities or polluted areas from entering the pulse sequence model.
2. The bionic leaf model is convenient to use, can be used for manufacturing a microfluidic chip in any season, and is suitable for popularization and use.
3. The bionic leaf model has good shape retention and long storage time, and is suitable for experimental research.
4. The bionic leaf model can keep the same shape and control variables in the follow-up experiment.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used in the embodiments or the description of the prior art will be briefly described below, and it is obvious that the drawings in the following description are only embodiments of the present invention, and that other drawings can be obtained according to the provided drawings without inventive effort for a person skilled in the art.
Fig. 1 is a schematic diagram of a preparation flow of a method according to an embodiment of the present invention. Wherein, figure A is a schematic diagram of fresh leaves; FIG. B is a schematic diagram of a female mold of the bionic leaf model; FIG. C is a schematic illustration of filling a cavity block with PDMS polymer; and D, a bionic leaf model obtained after solidification.
Fig. 2 is a physical diagram of a female die of a bionic leaf model mulberry leaf according to an embodiment of the invention.
Fig. 3 is a physical diagram of a bionic leaf model paulownia tomentosa She Yinmo according to an embodiment of the present invention.
Fig. 4 is a physical diagram of a male model of a bionic tree leaf model mulberry tree leaf according to an embodiment of the invention.
Fig. 5 is a diagram of a structural channel of an agarose microfluidic chip fabricated using a biomimetic leaf template.
Fig. 6 is a schematic diagram of a structural channel of an agarose microfluidic chip fabricated using a biomimetic leaf template.
Detailed Description
The following description of the technical solutions in the embodiments of the present invention will be clear and complete, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The PDMS mixture is prepared from the following adhesive A in percentage by mass: b glue = 10:1, wherein the type of the glue A and the type of the glue B are Michigan RTV615, and are purchased from Michigan Momentive in the United states.
The experimental methods and the detection methods described in the following examples are all conventional methods unless otherwise specified; the reagents and materials are commercially available unless otherwise specified.
As shown in fig. 1 to 4, a bionic leaf model capable of manufacturing a microfluidic chip without being limited by seasons comprises a model substrate and a pulse sequence, wherein the pulse sequence model is arranged on the model substrate, and the model substrate is in adsorption connection with the pulse sequence in an integrated manner; the bionic leaf model is formed by solidifying a PDMS polymer by means of fresh leaves.
The bionic leaf model can get rid of season limitation, and is convenient for experiments to carry out; can be repeatedly used, and protects the environment; the variables are controlled and the shape is kept unchanged. The bionic leaf model is applied to the manufacturing aspect of microfluidic chips.
When the microfluidic chip is used, the bionic leaf model is fixed, the PDMS polymer used for manufacturing the microfluidic chip is covered on the bionic leaf model, and after vacuumizing and bubble removal, solidification treatment is carried out to obtain a microfluidic channel; and bonding the microfluidic channel with a glass plate to obtain the microfluidic chip.
Example 1
A preparation method of a bionic leaf model capable of manufacturing a microfluidic chip without being limited by seasons comprises the following steps:
s1, fresh leaves are treated
Boiling picked fresh leaves in 75% ethanol solution for disinfection treatment, taking out after a period of time, and wiping with filter paper;
s2, PDMS Polymer configuration
The PDMS polymer is prepared from the following components in percentage by mass: b glue = 10:1, and mixing the materials in proportion. Glue A and glue B were model number Michaelk RTV615.
S3, preparation of bionic leaf model female die
Taking the back of the leaf as a male die, adhering double-sided adhesive tape on a glass plate, adhering the front of the leaf on the double-sided adhesive tape, covering a layer of PDMS polymer on the surface of the male die, vacuumizing in a vacuum drying oven to completely remove bubbles for 20min, ensuring that the mixed solution is polymerized to form hydrogel in a closed environment, then drying in a constant temperature and humidity room, curing at 80 ℃ for 45min, and tearing off the cured bionic leaf model female die from the leaf surface to obtain a bionic leaf model female die structure.
S4, female die modification of bionic leaf model
Pasting the female die of the bionic leaf model of the S3 above the inner part of the culture dish by using double faced adhesive tape, carrying 5 drops of modification liquid under the female die by using the culture dish, and sealing the culture dish; the operation is completed in a fume hood, and the fume hood is placed for 5 hours, so that the modification liquid is completely and naturally evaporated, and an isolation layer can be formed on the surface of the female die of the bionic leaf model. Wherein the modifying liquid is trichloro (1H, 2H-perfluorooctyl) silane.
S5, preparing a bionic leaf model finished product
The dish was opened, the female mold was removed, and the PDMS polymer was poured again, and after evacuating to completely remove bubbles for 20min, placed in an incubator at 80℃for 12h. At this time, the PDMS is separated from each other in the front and back two times due to the presence of the modifier, and an obvious isolation layer is provided, and the bionic leaf model structure is obtained after the PDMS is uncovered.
Application example 1
Fixing the bionic leaf model, covering PDMS polymer used for manufacturing the microfluidic chip on the bionic leaf model, vacuumizing to remove bubbles, and then curing to obtain a microfluidic channel; and bonding the microfluidic channel with the glass plate to obtain the microfluidic chip.
Application example 2
Fixing the bionic leaf model, covering melted agar on the bionic leaf model, vacuumizing to remove bubbles, and then solidifying to obtain a microfluidic channel; and bonding the microfluidic channel with the glass plate to obtain the microfluidic chip. In the present specification, each embodiment is described in a progressive manner, and each embodiment is mainly described in a different point from other embodiments, and identical and similar parts between the embodiments are all enough to refer to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (8)
1. The preparation method of the bionic leaf model is characterized by comprising the following steps of:
s1, fresh leaves are treated
Placing the picked fresh leaves into an ethanol solution for disinfection treatment, and then wiping the leaves with filter paper;
s2, preparation of bionic leaf model female die
Taking the back of the leaf as a male die, adhering double-sided adhesive tapes on a glass plate, adhering the front of the leaf on the double-sided adhesive tapes, covering a layer of PDMS polymer on the surface of the male die, vacuumizing to remove bubbles, and then performing curing treatment to obtain a bionic leaf model female die;
s3, female die modification of bionic leaf model
Placing the modified liquid at the bottom of the container, suspending the bionic leaf model female die prepared in the step S2 at the upper side of the modified liquid, sealing the container, standing to evaporate the modified liquid, and forming an isolation layer on the surface of the bionic leaf model female die;
s4, preparing a bionic leaf model finished product
Pouring PDMS polymer onto the S3 modified bionic leaf model female mold, vacuumizing to remove bubbles, and then curing to obtain a bionic leaf model with a pulse sequence structure;
in S3, the modification liquid is trichloro (1H, 2H-perfluorooctyl) silane.
2. The method for preparing a bionic leaf model according to claim 1, wherein in S1, the volume concentration of the ethanol solution is 70% -80%.
3. The method for preparing a bionic leaf model according to claim 1, wherein in S2, the curing process is as follows: curing at 75-80 deg.c for 40-45 min.
4. A method for preparing a biomimetic leaf model according to any one of claims 1 to 3, wherein in S4, the curing process is: curing for 11-12 h at 75-80 ℃.
5. The bionic leaf model prepared by the preparation method of claim 1, wherein the bionic leaf model comprises a model substrate and a pulse sequence model, the pulse sequence model is arranged on the model substrate, and the model substrate is in integral adsorption connection with the pulse sequence model.
6. The biomimetic leaf model of claim 5, wherein the pulse sequence model is arranged to be consistent with the pulse sequence of a picked fresh tree leaf.
7. The biomimetic leaf model of claim 5, wherein the different pulse sequence models are of a height consistent with the height of a picked fresh tree leaf pulse sequence.
8. The use of the biomimetic leaf model of claim 5 in the fabrication of microfluidic chips.
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Citations (6)
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JPS5672936A (en) * | 1979-11-19 | 1981-06-17 | Mitsubishi Electric Corp | Manufacture of board-shaped composite material composed of surface material formed into concavo-convex surface and lining material of foamed resin |
CN102923639A (en) * | 2012-08-08 | 2013-02-13 | 西安交通大学 | Precise molding method of biomimetic micro-channel system based on plant veins |
CN104517513A (en) * | 2013-12-14 | 2015-04-15 | 韦振忠 | Production method for simulated biological fossil specimen |
AU2020100527A4 (en) * | 2020-04-03 | 2020-05-14 | Zhonghang Monitoring Technology Research Institute Co., Ltd | Preparation technique for micro-nano structure and properties of bionic plant leaves by genetic morphology on surfaces of wood/bamboo-based decorative materials |
CN113086169A (en) * | 2021-03-30 | 2021-07-09 | 吉林大学 | Bionic propeller capable of reducing drag and noise and preparation method thereof |
CN113295191A (en) * | 2021-05-17 | 2021-08-24 | 广州大学 | Bionic bamboo leaf structure flexible strain sensor and preparation method and application thereof |
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2021
- 2021-09-27 CN CN202111135975.1A patent/CN113942154B/en active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5672936A (en) * | 1979-11-19 | 1981-06-17 | Mitsubishi Electric Corp | Manufacture of board-shaped composite material composed of surface material formed into concavo-convex surface and lining material of foamed resin |
CN102923639A (en) * | 2012-08-08 | 2013-02-13 | 西安交通大学 | Precise molding method of biomimetic micro-channel system based on plant veins |
CN104517513A (en) * | 2013-12-14 | 2015-04-15 | 韦振忠 | Production method for simulated biological fossil specimen |
AU2020100527A4 (en) * | 2020-04-03 | 2020-05-14 | Zhonghang Monitoring Technology Research Institute Co., Ltd | Preparation technique for micro-nano structure and properties of bionic plant leaves by genetic morphology on surfaces of wood/bamboo-based decorative materials |
CN113086169A (en) * | 2021-03-30 | 2021-07-09 | 吉林大学 | Bionic propeller capable of reducing drag and noise and preparation method thereof |
CN113295191A (en) * | 2021-05-17 | 2021-08-24 | 广州大学 | Bionic bamboo leaf structure flexible strain sensor and preparation method and application thereof |
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