CN113940902A - Amino acid additive of moisturizing cosmetics and preparation method thereof - Google Patents
Amino acid additive of moisturizing cosmetics and preparation method thereof Download PDFInfo
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- CN113940902A CN113940902A CN202010681250.1A CN202010681250A CN113940902A CN 113940902 A CN113940902 A CN 113940902A CN 202010681250 A CN202010681250 A CN 202010681250A CN 113940902 A CN113940902 A CN 113940902A
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- amino acid
- acid additive
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- 150000001413 amino acids Chemical class 0.000 title claims abstract description 26
- 239000002537 cosmetic Substances 0.000 title claims abstract description 18
- 239000000654 additive Substances 0.000 title claims abstract description 17
- 230000000996 additive effect Effects 0.000 title claims abstract description 17
- 230000003020 moisturizing effect Effects 0.000 title claims abstract description 17
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- 241001327916 Termitomyces albuminosus Species 0.000 claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000011324 bead Substances 0.000 claims abstract description 15
- 239000002994 raw material Substances 0.000 claims abstract description 13
- 108010093096 Immobilized Enzymes Proteins 0.000 claims abstract description 9
- 239000012535 impurity Substances 0.000 claims abstract description 9
- 238000000034 method Methods 0.000 claims abstract description 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 8
- 238000001914 filtration Methods 0.000 claims abstract description 3
- 229910052799 carbon Inorganic materials 0.000 claims abstract 2
- 210000002421 cell wall Anatomy 0.000 claims abstract 2
- 239000006228 supernatant Substances 0.000 claims description 9
- 238000002386 leaching Methods 0.000 claims description 6
- 108091005804 Peptidases Proteins 0.000 claims description 4
- 239000004365 Protease Substances 0.000 claims description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 239000008213 purified water Substances 0.000 claims description 4
- 241000208340 Araliaceae Species 0.000 claims description 3
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims description 3
- 235000003140 Panax quinquefolius Nutrition 0.000 claims description 3
- 239000003153 chemical reaction reagent Substances 0.000 claims description 3
- 235000008434 ginseng Nutrition 0.000 claims description 3
- 230000005484 gravity Effects 0.000 claims description 3
- 238000000265 homogenisation Methods 0.000 claims description 3
- 238000001471 micro-filtration Methods 0.000 claims description 3
- 239000002689 soil Substances 0.000 claims description 3
- 238000000108 ultra-filtration Methods 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 2
- 102000004190 Enzymes Human genes 0.000 claims description 2
- 239000006071 cream Substances 0.000 claims description 2
- 238000004042 decolorization Methods 0.000 claims description 2
- 239000000686 essence Substances 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 239000000243 solution Substances 0.000 claims 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 2
- 235000005550 amino acid supplement Nutrition 0.000 claims 2
- 239000006210 lotion Substances 0.000 claims 2
- 239000004909 Moisturizer Substances 0.000 claims 1
- 230000001153 anti-wrinkle effect Effects 0.000 claims 1
- 239000003963 antioxidant agent Substances 0.000 claims 1
- 239000007853 buffer solution Substances 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 claims 1
- 239000003974 emollient agent Substances 0.000 claims 1
- 239000003995 emulsifying agent Substances 0.000 claims 1
- 238000000605 extraction Methods 0.000 claims 1
- 239000003205 fragrance Substances 0.000 claims 1
- 230000001333 moisturizer Effects 0.000 claims 1
- 239000003755 preservative agent Substances 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
- 239000004094 surface-active agent Substances 0.000 claims 1
- 239000002562 thickening agent Substances 0.000 claims 1
- 239000011782 vitamin Substances 0.000 claims 1
- 229940088594 vitamin Drugs 0.000 claims 1
- 229930003231 vitamin Natural products 0.000 claims 1
- 235000013343 vitamin Nutrition 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 230000037303 wrinkles Effects 0.000 abstract description 2
- 238000004321 preservation Methods 0.000 abstract 1
- 210000003491 skin Anatomy 0.000 description 9
- 102000008186 Collagen Human genes 0.000 description 6
- 108010035532 Collagen Proteins 0.000 description 6
- 229920001436 collagen Polymers 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- 241000219357 Cactaceae Species 0.000 description 3
- 241000222680 Collybia Species 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 108010059892 Cellulase Proteins 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 229940106157 cellulase Drugs 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 241000228245 Aspergillus niger Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 244000056139 Brassica cretica Species 0.000 description 1
- 235000003351 Brassica cretica Nutrition 0.000 description 1
- 235000003343 Brassica rupestris Nutrition 0.000 description 1
- 235000011305 Capsella bursa pastoris Nutrition 0.000 description 1
- 240000008867 Capsella bursa-pastoris Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000006096 absorbing agent Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- QKSKPIVNLNLAAV-UHFFFAOYSA-N bis(2-chloroethyl) sulfide Chemical compound ClCCSCCCl QKSKPIVNLNLAAV-UHFFFAOYSA-N 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 239000000490 cosmetic additive Substances 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 210000004207 dermis Anatomy 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 210000001339 epidermal cell Anatomy 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 238000004880 explosion Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000003906 humectant Substances 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000010460 mustard Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000005408 paramagnetism Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 230000009759 skin aging Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/81—Preparation or application process involves irradiation
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Dermatology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Cosmetics (AREA)
Abstract
Disclosed herein are an amino acid additive for moisturizing cosmetics and a method for preparing the same, the amino acid additive being prepared by extracting termitomyces albuminosus, the method for preparing the extract comprising the steps of: moistening with water, breaking cell wall with high-pressure pulse, homogenizing and extracting under high pressure, performing enzymolysis with microwave-assisted magnetic bead immobilized enzyme, filtering to remove impurities, concentrating under reduced pressure, and decolorizing with active carbon. The amino acid additive has the effects of resisting wrinkle, nourishing, locking water and preserving moisture in cosmetics, and can be combined with other beneficial components to prepare preparations in different forms. At present, in the field of cosmetics, the application of the termitomyces albuminosus extract is still few, so that the invention provides a good scheme for the application of the termitomyces albuminosus plant raw material in the aspect of moisture preservation function.
Description
Technical Field
The invention relates to the field of cosmetic additives, in particular to an amino acid additive of a moisturizing cosmetic and a preparation method thereof.
Background
It is known that the main causes of aging of human skin are the loss of moisturizing factors, which causes the skin to have low water content, and the damage of oxygen free radicals to epidermal cells, wherein the oxygen free radicals oxidize nucleic acids through oxidation, so that the nucleic acids are broken and mutated, thereby affecting the normal transcription and translation of protein genetic information, and reducing or even eliminating the protein expression level. The loss of collagen causes the cracking of collagen peptide bonds and elastic nets supporting the skin, the spiral net structure of the collagen peptide bonds and elastic nets is damaged immediately, the skin tissue is oxidized, atrophied and collapsed, and the skin becomes old with dryness, wrinkles, looseness, inelasticity and the like. Therefore, collagen must be replenished to delay aging. Collagen is a highly hydrophilic substance, and abundant collagen can nourish much moisture in the dermis, so that the skin is increasingly plump and tight. The basic form of a protein is a protein polypeptide, and the constituent polypeptides are amino acids. The amino acid humectant exists in organism tissue naturally, is a natural moisturizing factor, has the characteristics of high purity, easy use, good stability and the like, and has excellent moisturizing effect and excellent moisturizing capability in cosmetics and detergent products.
The termitomyces albuminosus is called 'king in bacteria', the termitomyces albuminosus sporocarp contains abundant protein and comprehensive amino acid types, and the wild termitomyces albuminosus sporocarp contains 36.94% of protein and 8 essential amino acids required by human body. In recent years, with the development of cosmetic research technology, moisturizing function has been a hot issue in research in the field of cosmetics. The termitomyces albuminosus as a natural plant has the advantages of small toxic and side effects, high yield, strong functionality and the like, and the extract of the termitomyces albuminosus can be added as a moisture-preserving functional component. At present, the application of the termitomyces albuminosus extract is very little in the field of cosmetics, so that the termitomyces albuminosus functional plant raw material has wide market prospect in the field of cosmetics.
The invention Chinese patent CN 108635261A discloses a cactus enzymolysis fermentation broth and its preparation process and application in cosmetics, wherein the cactus enzymolysis fermentation broth is prepared from cactus mud, ethanol solution, Aspergillus niger, cellulase, glucose and purified water by enzymolysis and fermentation. The pretreatment technology of the method can not achieve the effect of wall breaking, so that the enzymolysis process is completed by using cellulase, and the method has the defect of high cost. Research on extracting amino acid from shepherd's purse, Schopper et al, cleaning, air drying, cutting, adding hydrochloric acid, heating under reflux, and hydrolyzing to obtain various amino acids. The method adopts acidolysis to treat mustard, and has the disadvantages of great pollution and difficult acid liquor treatment. Chinese patent CN 106420380A discloses a preparation method of skin moisturizing water containing silk amino acid, which comprises the steps of cleaning, mixing, enzymolysis, filtering, impurity removal, reduced pressure concentration, proportioning and the like, to obtain the skin moisturizing water containing the silk amino acid.
Disclosure of Invention
The invention aims to prevent and delay skin aging, reduce the loss of skin moisturizing factors and the like, and provides an amino acid additive of a moisturizing cosmetic and a preparation method thereof.
(1) Taking fresh termitomyces albuminosus, and washing with water to remove soil impurities;
(2) rehydrating the cleaned termitomyces albuminosus raw material in the step (1) with purified water for 0.1-1 h, putting the termitomyces albuminosus raw material into high-pressure pulse wall breaking equipment, quickly relieving pressure after the pressure is 0.2-2.0 MPa and the maintaining time is 0.5-10 min, and collecting termitomyces albuminosus discharged from a discharge opening;
(3) carrying out high-pressure homogenization treatment on the processed termitomyces albuminosus and ginseng raw materials in the step (2) for 10-100 min at the material-water ratio of 1:10 and the pressure of 40-100 Mpa to obtain a leaching solution;
(4) centrifuging the leaching solution obtained in the step (3) at a rotating speed of 5000r/min for 5-12 min, and taking supernatant for later use;
(5) adjusting the temperature of the supernatant obtained in the step (4) to 30-37 ℃, adjusting the pH to 6.5-8.0, adding magnetic bead immobilized enzyme according to 0.01-5% of the weight of the supernatant, carrying out microwave-assisted enzymolysis at the microwave temperature of 60 ℃, the microwave power of 500w, and the enzymolysis time of 1-15 min, and finally collecting the magnetic bead immobilized enzyme through an additional magnet to obtain an amino acid extracting solution;
(6) and (5) carrying out microfiltration and ultrafiltration on the extracting solution obtained in the step (5), removing impurities, carrying out reduced pressure concentration and activated carbon decolorization, and obtaining a concentrated solution with the specific gravity of 1.05-1.20.
The amino acid additive accounts for 0.5-25.0% of the total content of the composition.
The invention has the beneficial effects that:
(1) according to the invention, the termitomyces albuminosus raw material is processed by adopting a high-pressure pulse wall breaking technology, so that the wall breaking of the raw material can be realized, the dissolution of intracellular functional components is facilitated, more enzymolysis sites can be provided for the next enzymolysis part, and more moisturizing components can be added;
(2) the steam explosion technology is adopted, so that the sterilization effect is achieved, microorganisms bred in the treatment process can be killed, the probability of mildew is reduced, and the storage period is prolonged;
(3) the invention adopts microwave-assisted magnetic beads to immobilize enzyme for enzymolysis, the magnetic beads generally have super-strong paramagnetism, can be rapidly gathered in a magnetic field, have rich surface active groups so as to be coupled with protease, and realize the immobilized enzymolysis technology under the action of an external magnetic field. The magnetic beads are heat absorbers for microwave radiation, and the enzymolysis time is further reduced by combining the enzymolysis of the magnetic bead immobilized enzyme with the microwave radiation;
(4) the amino acid additive obtained by the invention has the functions of crease resistance, nourishing, water locking and moisture retention in cosmetics.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to examples, but those skilled in the art will appreciate that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention.
The examples, which are not labeled with specific conditions, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not labeled with manufacturers, and are all conventional products obtained by the market.
Preparation of termitomyces albuminosus extract
(1) Taking fresh termitomyces albuminosus, and washing with water to remove soil impurities;
(2) rehydrating the cleaned Collybia albuminosa raw material in the step (1) with purified water for 0.5 h, placing the rehydrated Collybia albuminosa raw material into high-pressure pulse wall breaking equipment, maintaining the pressure at 0.8 MPa for 3 min, quickly relieving pressure, and collecting Collybia albuminosa discharged from a discharge port;
(3) carrying out high-pressure homogenization treatment on the processed termitomyces albuminosus and ginseng raw materials in the step (2) for 30min at the material-water ratio of 1:10 and the pressure of 60Mpa to obtain leaching liquor;
(4) centrifuging the leaching solution obtained in the step (3) for 8 min at the rotating speed of 5000r/min, and taking the supernatant for later use;
(5) regulating the temperature of the supernatant obtained in the step (4) to 35 ℃, regulating the pH value to 7.0, adding magnetic bead immobilized enzyme according to 1% of the weight of the supernatant, carrying out enzymolysis with the assistance of microwave, wherein the microwave temperature is 60 ℃, the microwave power is 500w, and the enzymolysis time is 3 min, and finally collecting the magnetic bead immobilized enzyme through an additional magnet to obtain an amino acid extracting solution;
(6) and (5) carrying out microfiltration and ultrafiltration on the extracting solution obtained in the step (5), removing impurities, carrying out reduced pressure concentration and activated carbon decoloration to obtain a concentrated solution with the specific gravity of 1.10.
The amino acid additive accounts for 20.0% of the total composition.
Example 1
A toner was prepared as shown in Table 1 below
Watch 1
Example 2
The essences were prepared as shown in table 2 below
Watch two
Example 3
Emulsions were prepared as shown in Table 3 below
Watch III
Example 4
The creams were prepared as shown in table 4 below
Watch four
Example 5
The mask was prepared as shown in table 5 below
Watch five
Although the present invention has been described with reference to the preferred embodiments, it should be understood that the invention is not limited thereto, and that various changes and modifications can be made by those skilled in the art without departing from the spirit and scope of the invention.
Claims (6)
1. An amino acid additive of a moisturizing cosmetic and a preparation method thereof are characterized in that the amino acid additive is prepared by extracting termitomyces albuminosus, and the preparation method of the extract comprises the following steps: moistening water, breaking cell wall by high-pressure pulse, high-pressure homogenizing extraction, microwave-assisted magnetic bead immobilized enzyme enzymolysis, filtering to remove impurities, concentrating under reduced pressure, and decolorizing with active carbon
(1) Taking fresh termitomyces albuminosus, and washing with water to remove soil impurities;
(2) rehydrating the cleaned termitomyces albuminosus raw material in the step (1) with purified water for 0.1-1 h, putting the termitomyces albuminosus raw material into high-pressure pulse wall breaking equipment, quickly relieving pressure after the pressure is 0.2-2.0 MPa and the maintaining time is 0.5-10 min, and collecting termitomyces albuminosus discharged from a discharge opening;
(3) carrying out high-pressure homogenization treatment on the processed termitomyces albuminosus and ginseng raw materials in the step (2) for 10-100 min at the material-water ratio of 1:10 and the pressure of 40-100 Mpa to obtain a leaching solution;
(4) centrifuging the leaching solution obtained in the step (3) at a rotating speed of 5000r/min for 5-12 min, and taking supernatant for later use;
(5) adjusting the temperature of the supernatant obtained in the step (4) to 30-37 ℃, adjusting the pH to 6.5-8.0, adding magnetic bead immobilized enzyme according to 0.01-5% of the weight of the supernatant, carrying out microwave-assisted enzymolysis at the microwave temperature of 60 ℃, the microwave power of 500w, and the enzymolysis time of 1-15 min, and finally collecting the magnetic bead immobilized enzyme through an additional magnet to obtain an amino acid extracting solution;
(6) and (5) carrying out microfiltration and ultrafiltration on the extracting solution obtained in the step (5), removing impurities, carrying out reduced pressure concentration and activated carbon decolorization, and obtaining a concentrated solution with the specific gravity of 1.05-1.20.
2. An amino acid additive for moisturizing cosmetics and a preparation method thereof, wherein the amino acid additive accounts for 0.5-25.0% of the total content of the composition, and the additive is obtained by the method of claim 1.
3. The method of claim 1, wherein the enzyme is immobilized on the magnetic beads by mixing the magnetic bead reagent with the protease solution in a ratio of 1: 0.5-1, dissolving protease in 0.1M MES, 0.15M NaCl and a buffer solution with pH =6.0 to obtain a protease solution, wherein the magnetic bead reagent is a purchased kit.
4. The amino acid additive according to claim 1, which has anti-wrinkle, nourishing, water-retaining and moisturizing effects in cosmetics.
5. The cosmetic preparation forms to which the amino acid additive can be applied according to claim 1 include skin lotions, essences, lotions, creams and masks.
6. The amino acid supplement of claim 1, wherein the amino acid supplement is combined with other benefit agents including antioxidants, moisturizers, emulsifiers, emollients, thickeners, vitamins, surfactants, fragrances, preservatives, neutralizers and mixtures thereof.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010681250.1A CN113940902A (en) | 2020-07-15 | 2020-07-15 | Amino acid additive of moisturizing cosmetics and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010681250.1A CN113940902A (en) | 2020-07-15 | 2020-07-15 | Amino acid additive of moisturizing cosmetics and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113940902A true CN113940902A (en) | 2022-01-18 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010681250.1A Pending CN113940902A (en) | 2020-07-15 | 2020-07-15 | Amino acid additive of moisturizing cosmetics and preparation method thereof |
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| Country | Link |
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| CN (1) | CN113940902A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114939093A (en) * | 2022-06-14 | 2022-08-26 | 广东轻工职业技术学院 | Whitening and moisturizing composition containing amino acid and application of whitening and moisturizing composition in cosmetics |
-
2020
- 2020-07-15 CN CN202010681250.1A patent/CN113940902A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114939093A (en) * | 2022-06-14 | 2022-08-26 | 广东轻工职业技术学院 | Whitening and moisturizing composition containing amino acid and application of whitening and moisturizing composition in cosmetics |
| CN114939093B (en) * | 2022-06-14 | 2023-08-11 | 广州梵之容化妆品有限公司 | Whitening and moisturizing composition containing amino acid and application of composition in cosmetics |
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Application publication date: 20220118 |