CN113908091A - Anti-hair loss and hair-nourishing composition and preparation method and application thereof - Google Patents
Anti-hair loss and hair-nourishing composition and preparation method and application thereof Download PDFInfo
- Publication number
- CN113908091A CN113908091A CN202111198925.8A CN202111198925A CN113908091A CN 113908091 A CN113908091 A CN 113908091A CN 202111198925 A CN202111198925 A CN 202111198925A CN 113908091 A CN113908091 A CN 113908091A
- Authority
- CN
- China
- Prior art keywords
- hair
- extract
- hair loss
- parts
- composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 105
- 230000003656 anti-hair-loss Effects 0.000 title abstract description 29
- 238000002360 preparation method Methods 0.000 title abstract description 15
- 239000000284 extract Substances 0.000 claims abstract description 71
- 201000004384 Alopecia Diseases 0.000 claims abstract description 65
- 230000003676 hair loss Effects 0.000 claims abstract description 44
- 208000024963 hair loss Diseases 0.000 claims abstract description 44
- 210000004209 hair Anatomy 0.000 claims abstract description 37
- 230000003779 hair growth Effects 0.000 claims abstract description 33
- PJANXHGTPQOBST-VAWYXSNFSA-N Stilbene Natural products C=1C=CC=CC=1/C=C/C1=CC=CC=C1 PJANXHGTPQOBST-VAWYXSNFSA-N 0.000 claims abstract description 32
- -1 stilbene glucoside Chemical class 0.000 claims abstract description 32
- 235000021286 stilbenes Nutrition 0.000 claims abstract description 32
- MIJYXULNPSFWEK-GTOFXWBISA-N 3beta-hydroxyolean-12-en-28-oic acid Chemical compound C1C[C@H](O)C(C)(C)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CCC(C)(C)C[C@H]5C4=CC[C@@H]3[C@]21C MIJYXULNPSFWEK-GTOFXWBISA-N 0.000 claims abstract description 29
- KSDSYIXRWHRPMN-UHFFFAOYSA-N 4'-O-beta-D-Galactopyranoside-6''-p-Coumaroylprunin-4',5,7-Trihydroxyflavanone Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=C(C2OC3=CC(O)=CC(O)=C3C(=O)C2)C=C1 KSDSYIXRWHRPMN-UHFFFAOYSA-N 0.000 claims abstract description 29
- JKLISIRFYWXLQG-UHFFFAOYSA-N Epioleonolsaeure Natural products C1CC(O)C(C)(C)C2CCC3(C)C4(C)CCC5(C(O)=O)CCC(C)(C)CC5C4CCC3C21C JKLISIRFYWXLQG-UHFFFAOYSA-N 0.000 claims abstract description 29
- DEMKZLAVQYISIA-ONJCETCRSA-N Liquiritin Natural products O([C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1)c1ccc([C@@H]2Oc3c(C(=O)C2)ccc(O)c3)cc1 DEMKZLAVQYISIA-ONJCETCRSA-N 0.000 claims abstract description 29
- DEMKZLAVQYISIA-UHFFFAOYSA-N Liquirtin Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=C(C2OC3=CC(O)=CC=C3C(=O)C2)C=C1 DEMKZLAVQYISIA-UHFFFAOYSA-N 0.000 claims abstract description 29
- YBRJHZPWOMJYKQ-UHFFFAOYSA-N Oleanolic acid Natural products CC1(C)CC2C3=CCC4C5(C)CCC(O)C(C)(C)C5CCC4(C)C3(C)CCC2(C1)C(=O)O YBRJHZPWOMJYKQ-UHFFFAOYSA-N 0.000 claims abstract description 29
- MIJYXULNPSFWEK-UHFFFAOYSA-N Oleanolinsaeure Natural products C1CC(O)C(C)(C)C2CCC3(C)C4(C)CCC5(C(O)=O)CCC(C)(C)CC5C4=CCC3C21C MIJYXULNPSFWEK-UHFFFAOYSA-N 0.000 claims abstract description 29
- DEMKZLAVQYISIA-ZRWXNEIDSA-N liquiritin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C([C@H]2OC3=CC(O)=CC=C3C(=O)C2)C=C1 DEMKZLAVQYISIA-ZRWXNEIDSA-N 0.000 claims abstract description 29
- 229940100243 oleanolic acid Drugs 0.000 claims abstract description 29
- 230000003658 preventing hair loss Effects 0.000 claims abstract description 29
- HZLWUYJLOIAQFC-UHFFFAOYSA-N prosapogenin PS-A Natural products C12CC(C)(C)CCC2(C(O)=O)CCC(C2(CCC3C4(C)C)C)(C)C1=CCC2C3(C)CCC4OC1OCC(O)C(O)C1O HZLWUYJLOIAQFC-UHFFFAOYSA-N 0.000 claims abstract description 29
- GSZUGBAEBARHAW-UHFFFAOYSA-N sophoraflavone B Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=C(C=2OC3=CC(O)=CC=C3C(=O)C=2)C=C1 GSZUGBAEBARHAW-UHFFFAOYSA-N 0.000 claims abstract description 29
- 229930182478 glucoside Natural products 0.000 claims abstract description 22
- 230000001737 promoting effect Effects 0.000 claims abstract description 11
- 239000000243 solution Substances 0.000 claims description 38
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 241000382455 Angelica sinensis Species 0.000 claims description 12
- 244000021273 Peumus boldus Species 0.000 claims description 12
- 239000002537 cosmetic Substances 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 11
- 229930182470 glycoside Natural products 0.000 claims description 10
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 9
- SVTBMSDMJJWYQN-UHFFFAOYSA-N 2-methylpentane-2,4-diol Chemical compound CC(O)CC(C)(C)O SVTBMSDMJJWYQN-UHFFFAOYSA-N 0.000 claims description 8
- OWNRRUFOJXFKCU-UHFFFAOYSA-N Bromadiolone Chemical compound C=1C=C(C=2C=CC(Br)=CC=2)C=CC=1C(O)CC(C=1C(OC2=CC=CC=C2C=1O)=O)C1=CC=CC=C1 OWNRRUFOJXFKCU-UHFFFAOYSA-N 0.000 claims description 8
- 235000001287 Guettarda speciosa Nutrition 0.000 claims description 8
- 241000949456 Zanthoxylum Species 0.000 claims description 8
- 235000013399 edible fruits Nutrition 0.000 claims description 8
- 235000007650 Aralia spinosa Nutrition 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 7
- 239000011259 mixed solution Substances 0.000 claims description 7
- 238000002156 mixing Methods 0.000 claims description 7
- 239000002453 shampoo Substances 0.000 claims description 7
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims description 6
- 241000246044 Sophora flavescens Species 0.000 claims description 6
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 claims description 5
- 244000089698 Zanthoxylum simulans Species 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 229940051250 hexylene glycol Drugs 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 4
- 241000576429 Forsythia suspensa Species 0.000 claims description 3
- 239000006071 cream Substances 0.000 claims description 3
- ACCCMOQWYVYDOT-UHFFFAOYSA-N hexane-1,1-diol Chemical compound CCCCCC(O)O ACCCMOQWYVYDOT-UHFFFAOYSA-N 0.000 claims description 3
- XPFCZYUVICHKDS-UHFFFAOYSA-N 3-methylbutane-1,3-diol Chemical compound CC(C)(O)CCO XPFCZYUVICHKDS-UHFFFAOYSA-N 0.000 claims description 2
- CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical compound CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 claims description 2
- AEIJTFQOBWATKX-UHFFFAOYSA-N octane-1,2-diol Chemical compound CCCCCCC(O)CO AEIJTFQOBWATKX-UHFFFAOYSA-N 0.000 claims description 2
- 241000125175 Angelica Species 0.000 claims 2
- 229940013368 zanthoxylum bungeanum fruit extract Drugs 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 28
- 108010066551 Cholestenone 5 alpha-Reductase Proteins 0.000 abstract description 22
- 210000003780 hair follicle Anatomy 0.000 abstract description 17
- 108060008682 Tumor Necrosis Factor Proteins 0.000 abstract description 12
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 abstract description 12
- 230000002757 inflammatory effect Effects 0.000 abstract description 12
- MUMGGOZAMZWBJJ-DYKIIFRCSA-N Testostosterone Chemical compound O=C1CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 MUMGGOZAMZWBJJ-DYKIIFRCSA-N 0.000 abstract description 10
- 102000003777 Interleukin-1 beta Human genes 0.000 abstract description 8
- 108090000193 Interleukin-1 beta Proteins 0.000 abstract description 8
- 229960003604 testosterone Drugs 0.000 abstract description 5
- 108010080146 androgen receptors Proteins 0.000 abstract description 4
- 230000003778 catagen phase Effects 0.000 abstract description 4
- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 abstract 1
- 102100032187 Androgen receptor Human genes 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 14
- 238000012360 testing method Methods 0.000 description 14
- 230000000052 comparative effect Effects 0.000 description 12
- 210000003491 skin Anatomy 0.000 description 11
- NVKAWKQGWWIWPM-ABEVXSGRSA-N 17-β-hydroxy-5-α-Androstan-3-one Chemical compound C1C(=O)CC[C@]2(C)[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CC[C@H]21 NVKAWKQGWWIWPM-ABEVXSGRSA-N 0.000 description 10
- 241000699670 Mus sp. Species 0.000 description 9
- 210000004761 scalp Anatomy 0.000 description 9
- 229960003473 androstanolone Drugs 0.000 description 8
- 230000002401 inhibitory effect Effects 0.000 description 8
- 102000004190 Enzymes Human genes 0.000 description 7
- 108090000790 Enzymes Proteins 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 7
- 230000005764 inhibitory process Effects 0.000 description 7
- 244000061520 Angelica archangelica Species 0.000 description 6
- 239000002158 endotoxin Substances 0.000 description 6
- 229920006008 lipopolysaccharide Polymers 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 239000006228 supernatant Substances 0.000 description 6
- 231100000360 alopecia Toxicity 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 5
- 235000018102 proteins Nutrition 0.000 description 5
- 230000002195 synergetic effect Effects 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 5
- 241000193830 Bacillus <bacterium> Species 0.000 description 4
- 241000555712 Forsythia Species 0.000 description 4
- 235000015933 Peumus boldus Nutrition 0.000 description 4
- 238000002835 absorbance Methods 0.000 description 4
- 230000000844 anti-bacterial effect Effects 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 238000013329 compounding Methods 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 210000001732 sebaceous gland Anatomy 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 206010061218 Inflammation Diseases 0.000 description 3
- 241000699666 Mus <mouse, genus> Species 0.000 description 3
- ACFIXJIJDZMPPO-NNYOXOHSSA-N NADPH Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](OP(O)(O)=O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 ACFIXJIJDZMPPO-NNYOXOHSSA-N 0.000 description 3
- 102000001307 androgen receptors Human genes 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 230000004054 inflammatory process Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 3
- 239000000419 plant extract Substances 0.000 description 3
- 230000028327 secretion Effects 0.000 description 3
- 230000000638 stimulation Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 238000010998 test method Methods 0.000 description 3
- OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 description 2
- GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 description 2
- YTKGAYFHUZTLCI-UHFFFAOYSA-N 3-hydroxy-2-iminopyrimidin-4-amine Chemical compound NC1=CC=NC(=N)N1O YTKGAYFHUZTLCI-UHFFFAOYSA-N 0.000 description 2
- 208000002874 Acne Vulgaris Diseases 0.000 description 2
- 239000005739 Bordeaux mixture Substances 0.000 description 2
- 235000002566 Capsicum Nutrition 0.000 description 2
- 238000012286 ELISA Assay Methods 0.000 description 2
- 238000008157 ELISA kit Methods 0.000 description 2
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 2
- ZFMITUMMTDLWHR-UHFFFAOYSA-N Minoxidil Chemical compound NC1=[N+]([O-])C(N)=CC(N2CCCCC2)=N1 ZFMITUMMTDLWHR-UHFFFAOYSA-N 0.000 description 2
- 239000006002 Pepper Substances 0.000 description 2
- 235000016761 Piper aduncum Nutrition 0.000 description 2
- 240000003889 Piper guineense Species 0.000 description 2
- 235000017804 Piper guineense Nutrition 0.000 description 2
- 235000008184 Piper nigrum Nutrition 0.000 description 2
- 206010039792 Seborrhoea Diseases 0.000 description 2
- 102000004887 Transforming Growth Factor beta Human genes 0.000 description 2
- 108090001012 Transforming Growth Factor beta Proteins 0.000 description 2
- 206010000496 acne Diseases 0.000 description 2
- 239000003098 androgen Substances 0.000 description 2
- 230000003110 anti-inflammatory effect Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 229960004039 finasteride Drugs 0.000 description 2
- DBEPLOCGEIEOCV-WSBQPABSSA-N finasteride Chemical compound N([C@@H]1CC2)C(=O)C=C[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H](C(=O)NC(C)(C)C)[C@@]2(C)CC1 DBEPLOCGEIEOCV-WSBQPABSSA-N 0.000 description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
- 239000004519 grease Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000002055 immunohistochemical effect Effects 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 239000002504 physiological saline solution Substances 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 230000002028 premature Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 208000017520 skin disease Diseases 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- ZRKFYGHZFMAOKI-QMGMOQQFSA-N tgfbeta Chemical compound C([C@H](NC(=O)[C@H](C(C)C)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC(C)C)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](C)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1[C@@H](CCC1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O)C1=CC=C(O)C=C1 ZRKFYGHZFMAOKI-QMGMOQQFSA-N 0.000 description 2
- 229940098465 tincture Drugs 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 1
- LTTQKYMNTNISSZ-MWTRTKDXSA-N (2S)-(-)-kurarinone Chemical compound C1([C@H]2OC=3C(C[C@@H](CC=C(C)C)C(C)=C)=C(O)C=C(C=3C(=O)C2)OC)=CC=C(O)C=C1O LTTQKYMNTNISSZ-MWTRTKDXSA-N 0.000 description 1
- LOGFVTREOLYCPF-KXNHARMFSA-N (2s,3r)-2-[[(2r)-1-[(2s)-2,6-diaminohexanoyl]pyrrolidine-2-carbonyl]amino]-3-hydroxybutanoic acid Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)[C@H]1CCCN1C(=O)[C@@H](N)CCCCN LOGFVTREOLYCPF-KXNHARMFSA-N 0.000 description 1
- JAYVHSBYKLLDJC-DSNJPTTOSA-N (E)-2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside Chemical group O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C(O)C=C(O)C=C1\C=C\C1=CC=C(O)C=C1 JAYVHSBYKLLDJC-DSNJPTTOSA-N 0.000 description 1
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 description 1
- 244000025352 Artocarpus heterophyllus Species 0.000 description 1
- 235000008725 Artocarpus heterophyllus Nutrition 0.000 description 1
- 206010003694 Atrophy Diseases 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- GHOKWGTUZJEAQD-UHFFFAOYSA-N Chick antidermatitis factor Natural products OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 description 1
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
- 208000001840 Dandruff Diseases 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- VAPSMQAHNAZRKC-PQWRYPMOSA-N Epristeride Chemical compound C1C=C2C=C(C(O)=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](C(=O)NC(C)(C)C)[C@@]1(C)CC2 VAPSMQAHNAZRKC-PQWRYPMOSA-N 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- KFFCKOBAHMGTMW-LGQRSHAYSA-N Forsythin Chemical compound C1=C(OC)C(OC)=CC=C1[C@H]1[C@@H](CO[C@@H]2C=3C=C(OC)C(O[C@H]4[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O4)O)=CC=3)[C@@H]2CO1 KFFCKOBAHMGTMW-LGQRSHAYSA-N 0.000 description 1
- 235000007270 Gaultheria hispida Nutrition 0.000 description 1
- 108010068370 Glutens Proteins 0.000 description 1
- 241000243320 Hydrozoa Species 0.000 description 1
- ZSBXGIUJOOQZMP-UHFFFAOYSA-N Isomatrine Natural products C1CCC2CN3C(=O)CCCC3C3C2N1CCC3 ZSBXGIUJOOQZMP-UHFFFAOYSA-N 0.000 description 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 1
- ZSBXGIUJOOQZMP-JLNYLFASSA-N Matrine Chemical compound C1CC[C@H]2CN3C(=O)CCC[C@@H]3[C@@H]3[C@H]2N1CCC3 ZSBXGIUJOOQZMP-JLNYLFASSA-N 0.000 description 1
- 235000009134 Myrica cerifera Nutrition 0.000 description 1
- 244000269152 Myrica pensylvanica Species 0.000 description 1
- 235000012851 Myrica pensylvanica Nutrition 0.000 description 1
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 208000008469 Peptic Ulcer Diseases 0.000 description 1
- 240000002924 Platycladus orientalis Species 0.000 description 1
- 208000037062 Polyps Diseases 0.000 description 1
- 244000179560 Prunella vulgaris Species 0.000 description 1
- 208000003251 Pruritus Diseases 0.000 description 1
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
- 240000006079 Schisandra chinensis Species 0.000 description 1
- 235000008422 Schisandra chinensis Nutrition 0.000 description 1
- 206010039793 Seborrhoeic dermatitis Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- PDMMFKSKQVNJMI-BLQWBTBKSA-N Testosterone propionate Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H](OC(=O)CC)[C@@]1(C)CC2 PDMMFKSKQVNJMI-BLQWBTBKSA-N 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 1
- 208000037386 Typhoid Diseases 0.000 description 1
- 241000607626 Vibrio cholerae Species 0.000 description 1
- 229930003451 Vitamin B1 Natural products 0.000 description 1
- 229930003471 Vitamin B2 Natural products 0.000 description 1
- 229930003537 Vitamin B3 Natural products 0.000 description 1
- 229930003756 Vitamin B7 Natural products 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 210000000683 abdominal cavity Anatomy 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 229940069521 aloe extract Drugs 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 229940030486 androgens Drugs 0.000 description 1
- 230000037444 atrophy Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000036621 balding Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 108050002883 beta-defensin Proteins 0.000 description 1
- 102000012265 beta-defensin Human genes 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- RNFNDJAIBTYOQL-UHFFFAOYSA-N chloral hydrate Chemical compound OC(O)C(Cl)(Cl)Cl RNFNDJAIBTYOQL-UHFFFAOYSA-N 0.000 description 1
- 229960002327 chloral hydrate Drugs 0.000 description 1
- CCGSUNCLSOWKJO-UHFFFAOYSA-N cimetidine Chemical compound N#CNC(=N/C)\NCCSCC1=NC=N[C]1C CCGSUNCLSOWKJO-UHFFFAOYSA-N 0.000 description 1
- 229960001380 cimetidine Drugs 0.000 description 1
- 235000015165 citric acid Nutrition 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000005034 decoration Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 210000004207 dermis Anatomy 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 206010013023 diphtheria Diseases 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 229960004199 dutasteride Drugs 0.000 description 1
- JWJOTENAMICLJG-QWBYCMEYSA-N dutasteride Chemical compound O=C([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)N[C@@H]4CC3)C)CC[C@@]21C)NC1=CC(C(F)(F)F)=CC=C1C(F)(F)F JWJOTENAMICLJG-QWBYCMEYSA-N 0.000 description 1
- 208000001848 dysentery Diseases 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 229950009537 epristeride Drugs 0.000 description 1
- 229940011871 estrogen Drugs 0.000 description 1
- 239000000262 estrogen Substances 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 230000003721 exogen phase Effects 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 235000019152 folic acid Nutrition 0.000 description 1
- 239000011724 folic acid Substances 0.000 description 1
- 229960000304 folic acid Drugs 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 229940002508 ginger extract Drugs 0.000 description 1
- 235000020708 ginger extract Nutrition 0.000 description 1
- 235000020710 ginseng extract Nutrition 0.000 description 1
- 235000021312 gluten Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 210000003128 head Anatomy 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000007490 hematoxylin and eosin (H&E) staining Methods 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 201000010066 hyperandrogenism Diseases 0.000 description 1
- 238000013115 immunohistochemical detection Methods 0.000 description 1
- 230000003752 improving hair Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000016507 interphase Effects 0.000 description 1
- 230000007803 itching Effects 0.000 description 1
- 210000002510 keratinocyte Anatomy 0.000 description 1
- XQVFLLMCNGKXSM-UHFFFAOYSA-N kurarinone Natural products COc1cc(O)c(C(CC=C(C)C)C(=C)C)c2OC(CC(=O)c12)c3ccc(O)cc3O XQVFLLMCNGKXSM-UHFFFAOYSA-N 0.000 description 1
- 210000005228 liver tissue Anatomy 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 229930014456 matrine Natural products 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000004089 microcirculation Effects 0.000 description 1
- 229960003632 minoxidil Drugs 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004660 morphological change Effects 0.000 description 1
- KDADHLPROOOPIC-UHFFFAOYSA-N neokurarinol Natural products COc1cc(O)ccc1C1CC(=O)c2c(OC)cc(O)c(CC(CCC(C)(C)O)C(C)=C)c2O1 KDADHLPROOOPIC-UHFFFAOYSA-N 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- DFPAKSUCGFBDDF-UHFFFAOYSA-N nicotinic acid amide Natural products NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000037312 oily skin Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 206010033675 panniculitis Diseases 0.000 description 1
- 235000019161 pantothenic acid Nutrition 0.000 description 1
- 239000011713 pantothenic acid Substances 0.000 description 1
- 229940055726 pantothenic acid Drugs 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 208000011906 peptic ulcer disease Diseases 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 239000003910 polypeptide antibiotic agent Substances 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 235000010241 potassium sorbate Nutrition 0.000 description 1
- 239000004302 potassium sorbate Substances 0.000 description 1
- 229940069338 potassium sorbate Drugs 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 239000012460 protein solution Substances 0.000 description 1
- 230000009323 psychological health Effects 0.000 description 1
- RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 208000008742 seborrheic dermatitis Diseases 0.000 description 1
- 210000002374 sebum Anatomy 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 235000008113 selfheal Nutrition 0.000 description 1
- 235000020755 serenoa repens extract Nutrition 0.000 description 1
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 1
- 235000010234 sodium benzoate Nutrition 0.000 description 1
- 239000004299 sodium benzoate Substances 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000012192 staining solution Substances 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- PJANXHGTPQOBST-UHFFFAOYSA-N stilbene Chemical class C=1C=CC=CC=1C=CC1=CC=CC=C1 PJANXHGTPQOBST-UHFFFAOYSA-N 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 210000004304 subcutaneous tissue Anatomy 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 239000011885 synergistic combination Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 229960001712 testosterone propionate Drugs 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 230000003762 total hair loss Effects 0.000 description 1
- 201000008297 typhoid fever Diseases 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
- 229940118696 vibrio cholerae Drugs 0.000 description 1
- 235000010374 vitamin B1 Nutrition 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
- 235000019164 vitamin B2 Nutrition 0.000 description 1
- 239000011716 vitamin B2 Substances 0.000 description 1
- 235000019160 vitamin B3 Nutrition 0.000 description 1
- 239000011708 vitamin B3 Substances 0.000 description 1
- 235000019158 vitamin B6 Nutrition 0.000 description 1
- 239000011726 vitamin B6 Substances 0.000 description 1
- 235000011912 vitamin B7 Nutrition 0.000 description 1
- 239000011735 vitamin B7 Substances 0.000 description 1
- 229940011671 vitamin b6 Drugs 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/602—Glycosides, e.g. rutin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/63—Steroids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/14—Drugs for dermatological disorders for baldness or alopecia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/008—Preparations for oily hair
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/02—Preparations for cleaning the hair
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/12—Preparations containing hair conditioners
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q7/00—Preparations for affecting hair growth
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Dermatology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Cosmetics (AREA)
Abstract
The invention provides an anti-hair loss and hair-growing composition and a preparation method and application thereof. The hair loss preventing and hair growing composition mainly comprises: liquiritin, Bordeaux leaf extract, oleanolic acid and stilbene glucoside. The four components are mutually matched and synergized, so that the activity of the 5 alpha-reductase is effectively inhibited, the 5 alpha-reductase is greatly prevented from catalyzing testosterone to be converted into DHT, and the problems that the high-activity DHT is combined with an androgen receptor and then acts on hair follicles to generate the effect of rapidly entering a catagen, being miniaturized, falling off hairs and the like are solved; meanwhile, the anti-hair loss and hair-growing composition can also inhibit the expression of IL-1 beta and TNF-alpha inflammatory factors in skin hair follicles, so that the aims of preventing hair loss and promoting hair growth are finally fulfilled.
Description
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to an anti-hair loss and hair-growing composition and a preparation method and application thereof.
Background
The hair is an accessory of the human body, is an important component of the human body, and has protection, decoration and other physiological functions. Alopecia is a skin disorder characterized by hair loss. The hair loss causes hair sparseness or premature balding, the beauty is influenced, much trouble and pain are brought to patients, normal work and life of people are influenced, even the psychological health of the patients is disabled, the self-confidence of the people is struck, and the hair loss prevention by the healthy maintenance of the hair is an urgent problem to be solved by the people.
In the case of alopecia, one of the largest causative factors is abnormal sebaceous gland secretion. In response to sebaceous gland dyssecretosis, generally medically in response to hyperandrogenism, the use of increasing estrogen regulation or the use of some drugs such as cimetidine tincture against androgens that cause sebaceous gland dyssecretosis, and the use of finasteride, dutasteride and epristeride to inhibit 5 α -reductase. However, the use of hormonal drugs has various physical injuries. Therefore, the safety of the 5 α -reductase inhibitory drugs of the prior art has yet to be improved. For nursing oily skin, the main purposes are that the skin is clean and thorough, the secretion of grease is controlled, and the skin is in a water-oil balance state as much as possible. Meanwhile, the anti-inflammatory and antibacterial work is well done, and the skin diseases such as acne and seborrheic dermatitis can be effectively assisted and prevented and controlled.
CN110025775A discloses a hair loss preventing and hair restoring essence for mild hair fixation and a preparation method thereof, wherein the hair loss preventing and hair restoring essence for mild hair fixation comprises the following raw materials: water, ethanol, stilbene glucoside, glycerol, angelica sinensis extract, aloe extract, ginseng extract, tea polyphenol, corn gluten amino acids, vitamin H, folic acid, vitamin B3, pantothenic acid, vitamin B6, vitamin B2, vitamin B1, yeast polypeptides, cysteine, sodium benzoate, potassium sorbate, citric acid, linoleic acid, xylanase and polyp extract. According to the invention, linoleic acid, xylanase and hydroid extracts are adopted to synergistically improve the hair loss prevention rate and the hair growth promotion rate of the hair loss prevention and generation promotion shampoo for mild hair strengthening.
CN103446563A discloses a plant extract compound for preventing alopecia and inhibiting 5 alpha-reductase activity, belonging to a plant extract composition. The compound hair loss preventing plant compound comprises serenoa repens extract, waxberry bark extract, platycladus orientalis extract, Chinese angelica extract, rhizoma anemarrhenae extract, selfheal extract, ginger extract, radix sophorae flavescentis extract, jackfruit extract, schisandra chinensis extract and rhizoma kaempferiae extract, wherein the weight percentage content of each plant extract in the total weight of the hair loss preventing plant compound is 0.1-99%. The anti-hair loss plant compound is used for treating and improving the seborrheic alopecia symptoms caused by male androgen source.
Therefore, the development of a head care product for fundamentally solving the problem of hair loss is a research focus in the art.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide an anti-hair loss and hair-growing composition, a preparation method and application thereof. The anti-hair loss and hair-growing composition achieves the effects of controlling oil and inhibiting inflammatory factors by inhibiting the activity of 5 alpha-reductase, further achieves the effects of resisting inflammation and inhibiting bacteria, can realize the water-oil balance of scalp, and effectively repairs the barrier function of scalp, thereby radically solving the problem of hair loss.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the present invention provides an anti-hair loss hair growth composition comprising: liquiritin, Bordeaux leaf extract, oleanolic acid and stilbene glucoside.
In the invention, the liquiritin, the boldo tree leaf extracting solution, the oleanolic acid and the stilbene glucoside are mutually matched, so that a synergistic effect is achieved, the activity of 5 alpha-reductase is effectively inhibited, the 5 alpha-reductase is prevented from catalyzing testosterone to be converted into DHT to a great extent, and the problems that the high-activity DHT is combined with androgen receptors and then acts on hair follicles to generate the effect of rapidly entering a catagen stage, being miniaturized, falling hair and the like are avoided; meanwhile, the anti-hair loss and hair-growing composition can also inhibit the expression of IL-1 beta and TNF-alpha inflammatory factors in skin hair follicles, so that the aims of preventing hair loss and promoting hair growth are finally fulfilled.
In the invention, the liquiritin can inhibit peptic ulcer and prevent premature graying and alopecia, and can remarkably improve the anti-hair loss effect while diminishing inflammation, inhibiting bacteria, dissipating stagnation and relieving exterior syndrome when being added into the hair-care, anti-hair loss and hair-nourishing composition.
In the invention, the Bordeaux leaves extract activates the synthesis of skin beta-defensin (an endogenous antibacterial peptide), enhances the natural antibacterial system of the skin, reduces bacterial growth, inhibits inflammatory factors, refines pores and improves oily scalp.
In the invention, oleanolic acid can inhibit the activity of 5 alpha-reductase so as to achieve the oil control effect, and has the advantages of quick oil control effect, long oil control time and difficulty in repetition.
In the invention, the main component of the stilbene glucoside comprises the stilbene glucoside, the name of the stilbene glucoside is 2,3,5,4' -tetrahydroxy stilbene-2-O-beta-D-glucoside, the stilbene glucoside belongs to polyhydroxy stilbene compounds, and the stilbene glucoside can permeate into hairy roots and has the effects of sterilization, bacteriostasis and oxidation resistance.
Preferably, the hair loss preventing and hair growing composition comprises the following components in parts by weight: 0.01-1 part of liquiritin, 1-3 parts of Bordeaux leaf extract, 0.01-2 parts of oleanolic acid and 0.1-0.5 part of stilbene glycoside.
In the hair loss preventing and hair growing composition, the content of liquiritin is 0.01-1 part, for example, 0.01 part, 0.02 part, 0.05 part, 0.08 part, 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part and the like.
In the hair loss preventing and hair growing composition, the content of the bordeaux mixture extract is 1 to 3 parts, and may be, for example, 1 part, 1.2 parts, 1.4 parts, 1.6 parts, 1.8 parts, 2 parts, 2.2 parts, 2.4 parts, 2.6 parts, 2.8 parts, 3 parts, or the like.
The anti-hair loss and hair-growing composition may contain 0.01 to 2 parts of oleanolic acid, for example, 0.01, 0.05, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.8, 1, 1.2, 1.4, 1.6, 1.8, 2 parts thereof.
The content of stilbene glucoside in the hair loss preventing and growing composition is 0.1-0.5 part, for example, 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part and the like.
Preferably, the hair loss preventing and hair nourishing composition further comprises angelica sinensis extract, pepper fruit extract, sophora flavescens extract and forsythia suspensa extract.
In the invention, the anti-hair-loss and hair-nourishing composition is additionally added with an angelica sinensis extracting solution, a zanthoxylum bungeanum fruit extracting solution, a sophora flavescens extracting solution and a fructus forsythiae extracting solution, so that the capabilities of balancing scalp grease, reducing dandruff, sterilizing and inhibiting bacteria and preventing hair loss are further improved.
The main component of the angelica sinensis extract is angelica sinensis polysaccharide, which endows nutrient substances to the scalp and improves the nutrition transportation of the scalp, thereby recovering the functions of scalp epidermis, dermis and subcutaneous tissue cells and realizing the comprehensive health care of the scalp; the Chinese prickly ash fruit extracting solution is pungent and warm in nature and taste, warms the middle-jiao to relieve pain, kills parasites and relieves itching; matrine and kurarinone substances in the radix sophorae flavescentis extract are used as antibacterial ingredients in the hair restorer; the forsythia suspense extract contains the components of forsythol, forsythin A and the like, and has good antibacterial effect, and comprises streptococcus, vibrio cholerae, typhoid bacillus, diphtheria bacillus, escherichia coli, dysentery bacillus, staphylococcus and the like.
Preferably, the hair loss preventing and hair growing composition comprises the following components in parts by weight: 0.01-1 part of liquiritin, 1-3 parts of boldo leaf extract, 0.01-2 parts of oleanolic acid, 0.1-0.5 part of stilbene glycoside, 5-10 parts of angelica extract, 1-5 parts of pricklyash peel extract, 3-8 parts of radix sophorae flavescentis extract and 3-8 parts of fructus forsythiae extract.
The hair loss preventing and hair growing composition may contain 5 to 10 parts of the angelica sinensis extract, for example, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 9.5 parts, 10 parts, etc.
In the hair loss preventing and hair growing composition, the content of the pepper fruit extracting solution is 1 to 5 parts, and can be 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts and the like.
In the hair loss preventing and hair restoring composition, the content of the sophora flavescens extract is 3 to 8 parts, and can be 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts and the like.
In the hair loss preventing and hair growing composition, the content of the forsythia suspense extract is 3 to 8 parts, and for example, the forsythia suspense extract can be 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts and the like.
Preferably, the hair loss prevention and growth promotion composition further comprises a solvent, wherein the solvent is a mixed solution of alcohol and water.
Preferably, the mass ratio of the alcohol to the water is 1 (1-2), and may be, for example, 1:1, 1:1.1, 1:1.2, 1:1.3, 1:1.4, 1:1.5, 1:1.6, 1:1.7, 1:1.8, 1:1.9, 1:2, and the like.
Preferably, the alcohol comprises any one or a combination of at least two of ethanol, isoprene glycol, caprylyl glycol, propylene glycol, butylene glycol, hexylene glycol, or glycerol, preferably butylene glycol and/or hexylene glycol.
Preferably, the hair loss preventing and hair growing composition consists of the following components in percentage by mass: 0.01-1% of liquiritin, 1-3% of Bordeaux leaves extract, 0.01-2% of oleanolic acid, 0.1-0.5% of stilbene glycoside, 5-10% of Chinese angelica extract, 1-5% of Chinese prickly ash fruit extract, 3-8% of radix sophorae flavescentis extract, 3-8% of fructus forsythiae extract, 10-30% of butanediol, 10-30% of hexanediol and the balance of water.
The content of liquiritin is 0.01-1%, for example, 0.01%, 0.05%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1% or the like, based on 100% of the total weight of the hair loss preventing and growing composition.
The content of the bordeaux mixture extract is 1 to 3%, for example, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2%, 2.2%, 2.4%, 2.6%, 2.8%, 3%, etc., based on 100% by mass of the total hair loss preventing and growing composition.
The oleanolic acid content is 0.01 to 2% based on 100% of the total weight of the hair loss preventing and hair restoring composition, and may be, for example, 0.01%, 0.05%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5%, 0.6%, 0.8%, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2%, or the like.
The content of stilbene glucoside is 0.1-0.5%, for example, 0.1%, 0.2%, 0.3%, 0.4%, 0.5% and the like, based on 100% of the total mass of the composition.
The content of the angelica sinensis extract is 5 to 10%, for example, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5%, 10%, etc., based on 100% by mass of the hair loss preventing and growing composition.
The content of the extract of zanthoxylum bungeanum fruit is 1 to 5%, for example, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, etc., based on 100% of the total mass of the hair loss preventing and growing composition.
The content of the sophora flavescens ait extract is 3 to 8%, for example, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, etc., based on 100% of the total weight of the hair loss preventing and growing composition.
The content of the forsythia suspense extract is 3 to 8%, for example, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, etc., based on 100% of the total weight of the hair loss preventing and growing composition.
The content of the butylene glycol is 10 to 30%, for example, 10%, 12%, 14%, 16%, 18%, 20%, 22%, 24%, 26%, 28%, 30% or the like, based on 100% by mass of the hair loss preventing and growing composition.
The content of hexanediol is 10 to 30%, for example, 10%, 12%, 14%, 16%, 18%, 20%, 22%, 24%, 26%, 28%, 30%, etc., based on 100% by mass of the hair loss preventing and growing composition.
In a second aspect, the present invention provides a method for preparing the anti-alopecia hair-growing composition according to the first aspect, the method for preparing the anti-alopecia hair-growing composition comprising: dissolving liquiritin, Bordeaux leaves extracting solution, oleanolic acid and stilbene glucoside, and filtering to obtain the hair loss preventing and hair growing composition.
Preferably, the preparation method of the hair loss prevention and hair growth promotion composition comprises the following steps: dissolving liquiritin, Bordeaux leaves extracting solution, oleanolic acid, stilbene glucoside, angelica extracting solution, pricklyash peel extracting solution, radix sophorae flavescentis extracting solution and fructus forsythiae extracting solution in mixed solution of alcohol and water, mixing and stirring, and filtering to obtain the hair loss preventing and nourishing composition.
In a third aspect, the present invention provides a use of the hair loss prevention and hair growth promotion composition according to the first aspect in the preparation of cosmetics.
In a fourth aspect, the present invention provides a cosmetic comprising the anti-hair loss and hair growth composition according to the first aspect.
Preferably, the cosmetic comprises any one of shampoo, cream shampoo, essence or hair conditioner or the combination of at least two of the shampoo, the cream shampoo, the essence and the hair conditioner.
Preferably, the addition amount of the hair loss prevention and hair growth promotion composition is 1 to 20% of the total mass of the cosmetic, for example, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, etc., preferably 12 to 18%, and more preferably 15%.
Compared with the prior art, the invention has the following beneficial effects:
(1) the anti-hair loss and hair-growing composition disclosed by the invention effectively inhibits the activity of 5 alpha-reductase by synergistic compounding of four effective components, namely liquiritin, Bordeaux leaf extract, oleanolic acid and stilbene glucoside, so that the problem that the high-activity DHT acts on hair follicles after being combined with androgen receptors to quickly enter a catagen stage, is miniaturized, and shed hair and the like is avoided to a great extent;
(2) the anti-hair loss and hair-growing composition disclosed by the invention can inhibit the expression of IL-1 beta and TNF-alpha inflammatory factors in skin hair follicles by synergistic compounding of four effective components, namely liquiritin, Bordeaux leaf extract, oleanolic acid and stilbene glucoside, so that the aims of preventing hair loss and promoting hair growth are finally achieved;
(3) the hair loss preventing and nourishing composition can prevent and improve the condition of hair loss, keep the cortex sanitary, breathable and non-greasy, and can be used for converging excessively fat sebaceous glands, reducing sebum secretion and promoting blood circulation, and has the effects of cleaning and stimulating fallen hair pores.
Detailed Description
The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.
The following examples and comparative examples have the following sources of the components:
in the embodiment, the weight ratio of the angelica sinensis extract is [ < 5 >: 3 >
Example 1
This embodiment provides an anti-alopecia hair-growing composition, which comprises the following components in percentage by mass, based on 100% of the total mass of the anti-alopecia hair-growing composition:
the preparation method of the anti-hair loss and hair-nourishing composition comprises the following steps: dissolving liquiritin, boldo tree leaf extracting solution, oleanolic acid, stilbene glycoside, angelica extracting solution, pricklyash peel extracting solution, radix sophorae flavescentis extracting solution and fructus forsythiae extracting solution in a mixed solution of alcohol and water according to the formula ratio, mixing and stirring, and filtering to obtain the hair loss preventing and hair nourishing composition.
Example 2
This embodiment provides an anti-alopecia hair-growing composition, which comprises the following components in percentage by mass, based on 100% of the total mass of the anti-alopecia hair-growing composition:
the preparation method of the anti-hair loss and hair-nourishing composition comprises the following steps: dissolving liquiritin, boldo tree leaf extracting solution, oleanolic acid, stilbene glycoside, angelica extracting solution, pricklyash peel extracting solution, radix sophorae flavescentis extracting solution and fructus forsythiae extracting solution in a mixed solution of alcohol and water according to the formula ratio, mixing and stirring, and filtering to obtain the hair loss preventing and hair nourishing composition.
Example 3
This embodiment provides an anti-alopecia hair-growing composition, which comprises the following components in percentage by mass, based on 100% of the total mass of the anti-alopecia hair-growing composition:
the preparation method of the anti-hair loss and hair-nourishing composition comprises the following steps: dissolving liquiritin, boldo tree leaf extracting solution, oleanolic acid, stilbene glycoside, angelica extracting solution, pricklyash peel extracting solution, radix sophorae flavescentis extracting solution and fructus forsythiae extracting solution in a mixed solution of alcohol and water according to the formula ratio, mixing and stirring, and filtering to obtain the hair loss preventing and hair nourishing composition.
Examples 4 to 7
Examples 4 to 7 provide four hair loss preventing and hair growth promoting compositions, which are different from example 1 in that any one of an angelica extract, a zanthoxylum fruit extract, a sophora flavescens extract and a forsythia suspensa extract is absent, and the preparation method thereof is the same as example 1; the anti-hair loss and hair-growing composition for each example comprises the following components in percentage by mass:
comparative example 1
Comparative example 1 provides an anti-alopecia and hair-growing composition, which is different from example 1 in that liquiritin, boldo leaf extract, oleanolic acid and stilbene glucoside are not added, and the preparation method is the same as example 1; the anti-hair loss and hair-growing composition comprises the following components in percentage by mass:
comparative examples 2 to 5
Comparative examples 2 to 5 provide four hair loss prevention and hair growth promotion compositions, which are different from example 1 in that only one component selected from liquiritin, boldo leaf extract, oleanolic acid and stilbene glycoside is contained; the anti-hair loss and hair-growing composition for each example comprises the following components in percentage by mass:
comparative examples 6 to 8
Comparative examples 6 to 8 provide three hair loss prevention and hair growth promotion compositions, which are different from example 1 in that only two components of liquiritin, boldo leaf extract and oleanolic acid are mixed; the anti-hair loss and hair-growing composition for each example comprises the following components in percentage by mass:
comparative examples 9 to 11
Comparative examples 9 to 11 provide four hair loss prevention and hair growth promotion compositions, which are different from example 1 in that one component among liquiritin, boldo leaf extract, oleanolic acid is absent; the anti-hair loss and hair-growing composition for each example comprises the following components in percentage by mass:
test example 1
5 alpha reductase Activity inhibition assay
Test samples: the hair loss preventing and growing compositions prepared in examples 1 to 7 and comparative examples 1 to 11;
the test method comprises the following steps: the method specifically comprises the following steps:
(1) preparation of 5 α reduced crude enzyme:
the male SD rat is killed by 10% chloral hydrate and over-injected in the abdominal cavity, liver tissues are quickly dissected and taken out, excess blood is washed away by physiological saline, phosphate buffer (pH 7.4) is added according to the mass ratio of 1:5, magnetic beads are added to the mixture to be homogenized in a homogenizer, the homogenate is taken out and centrifuged for 10min at 10000r/min at 4 ℃ on a refrigerated centrifuge, the supernatant is taken out and centrifuged once again, the supernatant is crude enzyme liquid, and the crude enzyme liquid is frozen and stored for later use at-20 ℃.
(2) Protein content determination:
and (3) determining the protein concentration in the crude enzyme solution by using a protein quantitative kit, and diluting the protein standard substance into a series of standard protein solutions with the mass concentrations of 0, 10, 20, 40, 80, 120, 160 and 200 mu g/mL according to an experimental method provided by the kit. Adding 200 μ L of G250 staining solution into the above series of standard solutions, mixing, standing at room temperature for 3-5min, detecting absorbance at 595nm with microplate reader, and drawing standard curve with protein absorbance (Y) as ordinate and mass concentration (X, mg/mL) as abscissa. The crude enzyme protein mass concentration was calculated by the protein standard curve equation.
3)5 alpha reductase Activity inhibitory Activity assay
The total reaction volume of the 5 alpha reductase catalytic reaction is 200 mu L, and the total reaction volume is 120mg/mL liver homogenate, 5mg/mL NADPH, 0.2mg/mL testosterone and samples to be detected with different concentrations.
Blank group determination: adding 120 mu L of liver homogenate, 60 mu L of 5mg/mLNADPH, 10 mu L of 0.2mg/mL testosterone and 10 mu L of phosphate buffer (pH 7.4) into a 96-well plate, fully mixing, measuring the absorbance A0, incubating at the constant temperature of 37 ℃ for 60min, and measuring the absorbance A1 to obtain the NADPH change value delta A0.
Sample group determination: respectively preparing to-be-detected sample solutions (2, 5, 10, 20, 30 and 40mg/mL) with different concentrations, and respectively reacting to obtain the NADPH change value delta An.
And (4) positive group determination, namely determining whether the activity of the enzyme is inhibited or not by using finasteride as a positive control drug according to the method, and preliminarily calculating the inhibition rate.
The formula is calculated as I% (. DELTA.an-. DELTA.ao)/. DELTA.ao × 100%. The experiment was repeated 3 times, 3 replicates each time.
The specific test results are shown in Table 1
(wherein, the hair loss prevention and hair growth promotion composition is represented by the code C4, DPO represents diaminopyrimidine oxide, and both are diluted with water to make up to 100%, "-" represents the item not tested):
TABLE 1
The test results in table 1 show that the anti-hair loss and hair growth composition has an inhibition rate of 5 alpha reductase activity of over 180%, and fully shows that the anti-hair loss and hair growth composition can effectively penetrate into scalp and hair follicles through the synergistic compounding of four effective components, namely liquiritin, boldo leaf extract, oleanolic acid and stilbene glucoside, so that the activity of the 5 alpha reductase can be effectively inhibited, the 5 alpha reductase is prevented from catalyzing testosterone to be converted into DHT to a great extent, and the problems that the high-activity DHT acts on the hair follicles after being combined with androgen receptors to generate rapid entry into the catagen, miniaturization, hair loss and the like are solved.
In addition, in a dilution experiment, the anti-hair loss and hair growth promoting composition disclosed by the invention is suitable for cosmetics accounting for about 15% of the total mass of the cosmetics, not only can the inhibition rate of 5 alpha reductase activity be high, but also the defects and waste caused by excessive addition amount are avoided. Meanwhile, it is not difficult to find that the inhibition rate of the composition on the activity of the 5 alpha reductase can be further improved by additionally adding ethanol and/or diaminopyrimidine oxide at a low concentration (the addition amount of the C4 composition is 5-15%).
Test example 2
Inflammatory factor inhibition test
Test samples: the hair loss preventing and growing compositions prepared in examples 1 to 7 and comparative examples 1 to 11;
the test method comprises the following steps: lipopolysaccharide (LPS) is utilized to stimulate human immortalized keratinocytes (HaCaT cells) of an administration group and a blank group respectively, and protein expression of relevant inflammatory factors interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha) in supernatant of the HaCaT cells of a pretreatment group and a control group of the administration group is detected by ELISA.
HaCaT cells were routinely cultured in 10-containing mediaThe% (volume fraction) of fetal bovine serum was cultured in DMEM complete medium (supplemented with 1% diabody: penicillin 100U/mL and streptomycin 100. mu.g/mL) under conditions of 37 ℃ and 5% (volume fraction) CO2 in a saturated humidity incubator, with liquid change once every 2 to 3 days and passage once every 3 to 7 days. Taking HaCaT cells in logarithmic phase, digesting the cells with 0.25% trypsin-EDTA for 10min, cleaning the cells, adding a complete culture medium into the cell sediment, and gently blowing to obtain cell suspension. Fresh culture medium was used to adjust the cell concentration to 4X 104And (4) inoculating the cells to a 24-well plate, incubating for 24 hours in a cell incubator, and adding LPS after the adherence of the cells reaches about 60-70%. Setting a blank group, a Lipopolysaccharide (LPS) 20 mu g/mL stimulation 24h group, a pretreatment 24h + LPS 20 mu g/mL stimulation 24h group of different administration groups, setting 5 multiple holes in each group, sucking cell supernatant of each hole after stimulation is finished, subpackaging the cell supernatant into 1.5mL EP tubes, and immediately using or freezing at-20 ℃ for standby. The expression level of TNF-alpha and IL-1 beta in the supernatant was measured by ELISA kit method (Jiangsu enzyme-labeled Biotechnology Co., Ltd.).
The specific test results are shown in table 2:
TABLE 2
The test data in table 2 show that the hair loss prevention and hair growth promotion composition has a good inhibition effect on the generation of inflammatory factors, the content of the inflammatory factors IL-beta is reduced to below 125 after the hair loss prevention and hair growth promotion composition is used, and the content of the inflammatory factors TNF-alpha is reduced to below 126, so that the hair loss prevention and hair growth promotion composition fully shows that the hair loss prevention and hair growth promotion composition plays an anti-inflammatory role by the synergistic compounding of four effective components of liquiritin, Bordea leaf extract, oleanolic acid and stilbene glucoside, and simultaneously inhibits the IL-1 beta and TNF-alpha 2 inflammatory factors excessively expressed in skin hair follicles.
Test example 3
Mouse experiment
Test samples: the hair loss preventing and growing compositions prepared in examples 1 to 7 and comparative examples 1 to 11;
the test method comprises the following steps: c57 mice (male, 5 weeks old) were kept in an environment of temperature (23 + -2) ° C, humidity (54 + -10)% and free diet and drinking water. Except for a blank group, selecting the back of a mouse and injecting the drug subcutaneously, injecting testosterone propionate of 5 mg/kg. d, successfully modeling for 4 weeks and then administering, smearing normal saline on the model group and the blank group, smearing 5% minoxidil tincture of 0.2mL/d on the minoxidil group, smearing a corresponding 15 wt% of composition water solution on the sample group, 1 time every day and 4 weeks in a period.
(1) Ratio of terminal hair/vellus hair value: after the observation is finished in the 8 th week of the experiment, the mice are killed, the skin of the hair-shedding area at the back of each group of mice is taken and is flatly pasted on a fixed plate after being about 1cm multiplied by 1cm, paraffin section dewaxing, hematoxylin-eosin staining and dehydration mounting experiment operation are carried out, the morphological change of the hair follicles of the mice is observed under the 100-time microscope visual field and 3 non-overlapping visual fields on average, the length and the size of the hair follicles are measured by using Image-Pro Plus, and the ratio (A/T) of the number of the hair follicles in the growth phase to the number of the hair follicles in the resting phase is counted;
(2) ELISA assay of Dihydrotestosterone (DHT) and 5 α reductase content in skin tissue: dissecting the collected tissues of each group of mice to prepare tissue homogenate, measuring the content of DHT and 5 alpha-reductase in each group of samples by using an Elisa kit according to a method in an ELISA kit specification, recording data, and calculating the average value by using Excel;
(3) immunohistochemical determination of TGF-. beta.1 and TNF-. alpha.content in skin: taking 2.0cm multiplied by 0.8cm long-strip-shaped skin of each group of mice, placing the mice in physiological saline to fully rinse residual blood, spreading the residual blood on filter paper, drying the blood at room temperature, wrapping the mice with tin foil paper, carrying out an experiment for measuring the contents of TGF-beta 1 and TNF-alpha in the skin of the mice by immunohistochemical detection according to the using method of Anti-TGFB1 and TNF-alpha, recording data, and calculating the average value of the data by using Excel;
the specific test results are shown in table 3: (wherein, the hair loss prevention and hair growth promoting composition is represented by the reference numeral C4, "-" represents the item not tested):
TABLE 3
As can be seen from the test data in Table 3, the ratio of the terminal hair/vellus hair value of the HE staining result of the test area tissues on the back of the mouse is obviously increased to more than 4 after the anti-hair loss composition of the invention is used; the ELISA assay shows that the content of Dihydrotestosterone (DHT) and 5 alpha reductase in skin tissues is obviously reduced, and the immunohistochemical assay shows that the content of TGF-beta 1 and TNF-alpha in skin is obviously reduced.
Therefore, the synergistic combination of the liquiritin, the boldo leaf extracting solution, the oleanolic acid and the stilbene glucoside can effectively penetrate into the scalp and hair follicles, effectively inhibit 5 alpha-reductase and improve hair follicle atrophy caused by DHT (DHT); meanwhile, IL-1 beta and TNF-alpha inflammatory factors which are over-expressed due to the breeding of anaerobic bacteria acne bacillus in skin hair follicles are inhibited, the effects of controlling oil and resisting inflammation can be achieved by treating both symptoms and root causes, the microcirculation of the hair follicles is fundamentally improved, the cell metabolism is stimulated, and the hair loss problem is fundamentally solved.
The applicant states that the present invention is illustrated by the above examples to show the hair loss prevention and hair growth promotion composition of the present invention, and the preparation method and application thereof, but the present invention is not limited to the above examples, i.e. it is not meant that the present invention is implemented only by relying on the above examples. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.
Claims (10)
1. An anti-alopecia hair-growing composition, comprising: liquiritin, Bordeaux leaf extract, oleanolic acid and stilbene glucoside.
2. The hair loss prevention and hair growth composition of claim 1, wherein the hair loss prevention and hair growth composition comprises in parts by weight: 0.01-1 part of liquiritin, 1-3 parts of Bordeaux leaf extract, 0.01-2 parts of oleanolic acid and 0.1-0.5 part of stilbene glycoside.
3. The hair loss prevention and hair growth promoting composition according to claim 1 or 2, further comprising an angelica sinensis extract, a zanthoxylum bungeanum fruit extract, a sophora flavescens extract and a forsythia suspensa extract.
4. The anti-alopecia hair-growing composition according to any one of claims 1-3, wherein the anti-alopecia hair-growing composition comprises, in parts by weight: 0.01-1 part of liquiritin, 1-3 parts of boldo leaf extract, 0.01-2 parts of oleanolic acid, 0.1-0.5 part of stilbene glycoside, 5-10 parts of angelica extract, 1-5 parts of pricklyash peel extract, 3-8 parts of radix sophorae flavescentis extract and 3-8 parts of fructus forsythiae extract.
5. The anti-alopecia hair-growing composition according to any one of claims 1 to 4, further comprising a solvent, wherein the solvent is a mixed solution of alcohol and water;
preferably, the mass ratio of the alcohol to the water is 1 (1-2);
preferably, the alcohol comprises any one or a combination of at least two of ethanol, isoprene glycol, caprylyl glycol, propylene glycol, butylene glycol, hexylene glycol, or glycerol, preferably butylene glycol and/or hexylene glycol.
6. The hair loss prevention and hair growth promoting composition according to any one of claims 1 to 5, wherein the hair loss prevention and hair growth promoting composition comprises the following components in percentage by mass: 0.01-1% of liquiritin, 1-3% of Bordeaux leaves extract, 0.01-2% of oleanolic acid, 0.1-0.5% of stilbene glycoside, 5-10% of Chinese angelica extract, 1-5% of Chinese prickly ash fruit extract, 3-8% of radix sophorae flavescentis extract, 3-8% of fructus forsythiae extract, 10-30% of butanediol, 10-30% of hexanediol and the balance of water.
7. The method of preparing the anti-alopecia hair-growing composition according to any one of claims 1-6, wherein the method of preparing the anti-alopecia hair-growing composition comprises: dissolving liquiritin, Bordeaux leaves extracting solution, oleanolic acid and stilbene glucoside, and filtering to obtain the hair loss preventing and hair growing composition.
8. The method of preparing the hair loss prevention and hair growth composition of claim 7, wherein the method of preparing the hair loss prevention and hair growth composition comprises: dissolving liquiritin, Bordeaux leaves extracting solution, oleanolic acid, stilbene glucoside, angelica extracting solution, pricklyash peel extracting solution, radix sophorae flavescentis extracting solution and fructus forsythiae extracting solution in mixed solution of alcohol and water, mixing and stirring, and filtering to obtain the hair loss preventing and nourishing composition.
9. Use of the anti-alopecia hair-care composition according to any one of claims 1-6 for preparing a cosmetic.
10. A cosmetic comprising the hair loss preventing and hair growth promoting composition according to any one of claims 1 to 6;
preferably, the cosmetic comprises any one or combination of at least two of shampoo, cream shampoo, essence or hair conditioner;
preferably, the addition amount of the hair loss prevention and hair growth promotion composition accounts for 1-20%, preferably 12-18%, and more preferably 15% of the total mass of the cosmetic.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202111198925.8A CN113908091B (en) | 2021-10-14 | 2021-10-14 | Anti-hair-loss hair-growing composition and preparation method and application thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202111198925.8A CN113908091B (en) | 2021-10-14 | 2021-10-14 | Anti-hair-loss hair-growing composition and preparation method and application thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
CN113908091A true CN113908091A (en) | 2022-01-11 |
CN113908091B CN113908091B (en) | 2023-10-27 |
Family
ID=79240330
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202111198925.8A Active CN113908091B (en) | 2021-10-14 | 2021-10-14 | Anti-hair-loss hair-growing composition and preparation method and application thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN113908091B (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114209632A (en) * | 2022-01-26 | 2022-03-22 | 拉芳家化股份有限公司 | Pre-washing hair-fixing essential oil |
CN115212144A (en) * | 2022-07-24 | 2022-10-21 | 广东爱宝日化科技有限公司 | Stable and effective bacteriostatic, itching-relieving and alopecia-preventing composition as well as preparation and application thereof |
WO2024052756A1 (en) * | 2022-09-09 | 2024-03-14 | Crodarom | Use of an extract of sichuan pepper for a skin treatment and a composition suitable for this use. |
FR3139466A1 (en) * | 2022-09-09 | 2024-03-15 | Crodarom | Use of a Sichuan pepper extract for skin treatment and a composition adapted to this use. |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104918603A (en) * | 2012-12-27 | 2015-09-16 | 株式会社林原 | Skin-exterior anti-ageing composition and production method therefor |
CN107281185A (en) * | 2017-06-16 | 2017-10-24 | 曹凡 | A kind of trichogenous emulsification composition and preparation method thereof |
CN112220719A (en) * | 2020-10-30 | 2021-01-15 | 花安堂生物科技集团有限公司 | Hair-blacking composition and preparation method and application thereof |
-
2021
- 2021-10-14 CN CN202111198925.8A patent/CN113908091B/en active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104918603A (en) * | 2012-12-27 | 2015-09-16 | 株式会社林原 | Skin-exterior anti-ageing composition and production method therefor |
CN105585601A (en) * | 2012-12-27 | 2016-05-18 | 株式会社林原 | Skin-Exterior Anti-Ageing Composition And Production Method Therefor |
CN107281185A (en) * | 2017-06-16 | 2017-10-24 | 曹凡 | A kind of trichogenous emulsification composition and preparation method thereof |
CN112220719A (en) * | 2020-10-30 | 2021-01-15 | 花安堂生物科技集团有限公司 | Hair-blacking composition and preparation method and application thereof |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114209632A (en) * | 2022-01-26 | 2022-03-22 | 拉芳家化股份有限公司 | Pre-washing hair-fixing essential oil |
CN114209632B (en) * | 2022-01-26 | 2023-06-27 | 拉芳家化股份有限公司 | Pre-washing hair-fixing essential oil |
CN115212144A (en) * | 2022-07-24 | 2022-10-21 | 广东爱宝日化科技有限公司 | Stable and effective bacteriostatic, itching-relieving and alopecia-preventing composition as well as preparation and application thereof |
CN115212144B (en) * | 2022-07-24 | 2023-06-23 | 广州市比柔生物科技有限公司 | Stable and effective antibacterial itching-relieving alopecia-preventing composition and preparation and application thereof |
WO2024052756A1 (en) * | 2022-09-09 | 2024-03-14 | Crodarom | Use of an extract of sichuan pepper for a skin treatment and a composition suitable for this use. |
FR3139466A1 (en) * | 2022-09-09 | 2024-03-15 | Crodarom | Use of a Sichuan pepper extract for skin treatment and a composition adapted to this use. |
FR3139467A1 (en) * | 2022-09-09 | 2024-03-15 | Crodarom | Use of a Sichuan pepper extract for skin treatment and a composition adapted to this use. |
Also Published As
Publication number | Publication date |
---|---|
CN113908091B (en) | 2023-10-27 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN113908091A (en) | Anti-hair loss and hair-nourishing composition and preparation method and application thereof | |
KR100992565B1 (en) | Topical preparations for hair growth | |
CN103282041A (en) | Extract of aerial parts of maca rich in polyphenols and composition comprising same | |
MX2007007957A (en) | Composition based on vegetal extracts of ajuga reptans for preventing hair loss, stimulating the growth of hair, regulating the production of sebum. | |
MX2007014481A (en) | System and method for promoting hair growth and improving hair and scalp health. | |
KR102021463B1 (en) | External composition for skin containing Ginsenoside Rg3 | |
KR102311987B1 (en) | Cosmetic composition for preventing hair loss and promoting hair growth | |
KR101690760B1 (en) | The composition of Caffeoylpentapeptide Cosmetic that has the Constitution of skin Anti-irritant for the Prevention of Hair Loss and the Promotion of Hair Growth | |
CN113750014A (en) | Anti-hair loss and hair-nourishing composition and preparation method and application thereof | |
CN112870333A (en) | Composition and preparation for improving alopecia and promoting hair growth of human, and preparation method and application thereof | |
KR20060095062A (en) | Hair growth agent composition | |
KR101698922B1 (en) | Composition for promoting hair growth and restoration | |
KR101965595B1 (en) | Composition for prevention of losing hair or promotion of growing hair | |
JPH07126146A (en) | Sin external preparation | |
KR20070067256A (en) | Composition for growing hair and preventing fall-out of hair | |
KR20140145278A (en) | Cosmetic composition containing Cimicifuga heracleifolia extract, Cornus officinalis extract and Geranium nepalense extract for skin convergence and elasticity effect | |
JP2014185131A (en) | Hair growing agent and hair cosmetic for hair growth | |
KR102024572B1 (en) | External composition for skin containing Ginsenoside Rf | |
KR100992414B1 (en) | Cosmetic compositions for prevention of hair loss or hair restoration containing extracts of fig leaves | |
CN113750013A (en) | Anti-hair loss and hair-nourishing composition and preparation method and application thereof | |
JP2002284648A (en) | Composition for hair restorer | |
KR100331675B1 (en) | External skin preparation | |
KR20080011970A (en) | Compositions for prevention of hair loss or hair restoration containing extracts of pueraria plants | |
KR102528199B1 (en) | Composition for preventing hair loss and promoting hair growth | |
JP7315940B2 (en) | fibroblast growth factor-5 expression inhibitor |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |