CN113875704A - Indoor propagation method for hornet - Google Patents
Indoor propagation method for hornet Download PDFInfo
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- CN113875704A CN113875704A CN202111282624.3A CN202111282624A CN113875704A CN 113875704 A CN113875704 A CN 113875704A CN 202111282624 A CN202111282624 A CN 202111282624A CN 113875704 A CN113875704 A CN 113875704A
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention relates to an indoor propagation method of hornet mustards, which comprises the steps of firstly, indoor feeding pupae of the hornet mustards to obtain first-generation seed bees, then, accessing the first-generation seed bees to an insect cage, and then, putting a turnover box connected with host spodoptera exigua larvae into the insect cage to carry out co-feeding of parasitic wasps and hosts; after a period of time, taking out the turnover box and placing the turnover box in a new insect-raising cage, replacing the original insect-raising cage with a new host to continue parasitizing, and repeating the steps until all the parasitic female bees die; collecting parasitic bee cocoons which grow to pupal stage from the parasitic cocoons, culturing the parasitic bee cocoons until emergence, and using the parasitic bee cocoons as seed bees for breeding parasitic bees of the next generation. According to the method, the asparagus caterpillar larvae are used as hosts, the hornet is parasitized and generates a large number of offspring, and the hornet can be stored at low temperature and normal temperature in a pupa form, so that indoor large-scale propagation of the hornet is realized, an insect source is provided for field release application of the hornet, and technical support is provided for biological control of noctuidae pests.
Description
Technical Field
The invention belongs to the technical field of parasitic wasp propagation, and particularly relates to an indoor propagation method for hornet.
Background
Beet armyworm (Spodoptera exigua Hubner) is an important pest with a very wide occurrence range, mainly harms 138 crops of 108 genera of 35 families such as vegetables, tobacco, cotton, corn, soybean and the like, and the pests of the families eat plant leaves and stalks by larvae to cause great yield reduction of the crops. At present, chemical pesticides are still the main means for controlling beet pulp. With the increase of the attention degree of people to food safety, the increase of the demand of pollution-free green food and the use of chemical prevention, the problems that plant pesticide residues exceed standards, the drug resistance of pests is enhanced, secondary pests rise to main pests and the like become more serious day by day. The natural enemy pest control in the biological control means is a green and safe pest control means. Parasitic wasps are an important biological control resource, and the parasitic wasps inject eggs into hosts, depend on the hosts to enable offspring of the parasitic wasps to grow and develop, and finally cause the death of the hosts. The artificial breeding and field release of the natural enemies can increase the number of the natural enemies in the nature, and further increase the parasitic number of target pests, thereby achieving the aim of controlling the base number of the pests, reducing the use of chemical pesticides and improving the yield and quality of agricultural products.
Hornet (snellensis Ashmead) belongs to family Hymenoptera braconidae. The bee is an endoparasitic bee, and indoor test results show that the bee has higher parasitic rate on spodoptera exigua larvae and prodenia litura larvae of noctuidae, has better pest control effect, and provides theoretical basis for biological control of the bee on noctuidae pests.
At present, the application of parasitic wasps in China is mainly trichogramma, the artificial propagation and release application of the trichogramma is high in the level of preventing and treating the ostrinia nubilalis, and few research reports about the indoor propagation technology of the hornet at home and abroad are reported. The problems existing in the prior art are that: parasitic wasps can be bred indoors, but cannot be propagated, however, the key technology for preventing and controlling pests by using the parasitic wasps is that the parasitic wasps can be artificially propagated in large quantities and can be used as important factors for biological prevention and control, so that the research on the indoor propagation method of the hornet bombycis is urgent.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides an indoor propagation method for hornworts horneri. According to the method, the asparagus caterpillar is selected as a host, indoor large-scale breeding of the hornet nest is realized, after the parasitic wasp pupates, the hornet nest can be stored and transported at a low temperature and used in field release, an insect source is provided for a large amount of hornet nest applied to agricultural production, and technical support is provided for biological control of noctuidae pests.
The technical scheme adopted by the invention is as follows:
an indoor propagation method for hornworts cunea kuntze, comprising the following steps:
(1) pupae of the Marira abrupt-chest cocoons are taken to be bred indoors, and the pupae are taken as first generation of breeding bees after parasitic bees emerge;
(2) inoculating the first generation of bee in the step (1) into an insect breeding cage, then putting a turnover box connected with host spodoptera exigua larvae into the insect breeding cage, and putting the turnover box into an artificial climate chamber for co-breeding parasitic bees and hosts; the first generation of breeding bees include adult female parasitic bees and adult male parasitic bees;
(3) after parasitic wasps and hosts are co-bred for a period of time, taking out the turnover box and placing the turnover box in a new insect breeding cage, replacing the new hosts with the original insect breeding cage for continuous parasitism, replacing the hosts once every a period of time, and repeating the steps until all the parasitic wasps of females die;
(4) collecting parasitic wasp cocoons which grow to pupal stage after parasitization, placing the parasitic wasps in an artificial climate chamber for culturing until emergence, and using the parasitic wasps after emergence as seed wasps for breeding parasitic wasps of the next generation.
In the step (1), the indoor breeding temperature is 25 +/-1 ℃, the relative humidity is 75-80%, and the photoperiod is L: D-14: 10 h.
In the step (2), the first generation of bee is a parasitic bee imago from 2 nd to 3 rd of emergence, and the male-female ratio of the parasitic bee imago is 1:1-8: 1.
In the step (2), the host beet armyworm larvae are 1-3 instar larvae of the host beet armyworm.
In the step (2), when co-feeding is carried out, the ratio of the number of heads of the host beet armyworm larvae to the number of heads of the parasitic wasp female adults is 1:1-10: 1.
In the step (2), the conditions in the artificial climate chamber are as follows: the temperature is 25 +/-1 ℃, the relative humidity is 75-80%, and the photoperiod is L: d is 14:10 h.
In the step (3), the parasitic wasp and the host are co-bred for 12-48h, and the host is replaced every 12-48 h.
In the step (4), parasitic wasp cocoons which grow to the pupal stage after being parasitized are collected in the fingering tube, the opening of the tube is sealed by a sponge plug, the parasitized wasp cocoons are continuously cultured in an artificial climate chamber, after the parasitized wasp cocoons are eclosized, the parasitized wasp cocoons are fed by cotton balls containing 20% of honey water or 10% of cane sugar water for nutrition, and the parasitized wasps are used for breeding of the next generation after the eclosion 3 d.
The turnover box is made of plastic materials, the length, the width and the height of the turnover box are 32.00cm, 24.30cm, 13.00cm, 1 rectangular basket with the length, the width and the height of 31.80cm, the width and the height of 22.00cm and the height of 8.50cm is placed in the turnover box, pea seedlings are planted in the rectangular basket, 1-3-year-old larvae of the beet armyworms are connected with the rectangular basket, water is added into the turnover box and submerges the roots of the pea seedlings, and the rectangular basket and the turnover box are separated and have water, so that the beet armyworms are effectively prevented from climbing out.
The turnover box is made of plastic, the length, the width and the height are 32.00cm, 24.30cm, 13.00cm, nutrition formula soil is filled in the turnover box, the nutrition formula soil comprises 4.00kg of soil, 80.00g of perlite and 110.00g of vermiculite, then 150 and 100-year old asparagus caterpillars soaked for 1 day are planted in the nutrition formula soil, and after the asparagus seedlings grow out, the asparagus caterpillars with 1-3 years old asparagus caterpillars are inoculated.
The Marnila steep chest cocoon bee pupa obtained by propagation by the method is stored in a refrigerator at 4 ℃ for diapause for use when released in the field, and experiments show that the emergence rate is over 75 percent when the pupa of the bee is stored in the refrigerator at 4 ℃ for 14 days. Storing for 5-7 days at room temperature of 25 deg.C and humidity of 70-80%, with eclosion rate of above 80%.
The invention has the following beneficial effects:
the indoor propagation method of the hornet of the manila steep chest is characterized in that pupas of the hornet of the manila steep chest are firstly bred indoors to obtain first generation seed bees, then the first generation seed bees are connected into an insect breeding cage, and then a turnover box connected with host spodoptera exigua larvae is placed into the insect breeding cage to carry out co-breeding of parasitic wasps and hosts; after a period of time, taking out the turnover box and placing the turnover box in a new insect-raising cage, replacing the new host with the original insect-raising cage for continuous parasitism, replacing the host once every period of time, and repeating the steps until all the parasitic female bees die; collecting parasitic bee cocoons which grow to pupal stage from the parasitic cocoons, culturing the parasitic bee cocoons until emergence, and using the parasitic bee cocoons as seed bees for breeding parasitic bees of the next generation. According to the method, the asparagus caterpillar larvae are used as hosts, the hornet bees with steep chest can parasitize in a large quantity and generate a large number of offspring, and the hornet bees with steep chest can be stored at low temperature and normal temperature in a pupa form, so that indoor large-scale propagation of the hornet bees with steep chest is realized, an insect source is provided for field release application of the hornet bees, and technical support is provided for biological control of noctuidae pests.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the embodiments described are merely exemplary of the invention, and not of the invention in its entirety. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention.
Example 1
The embodiment provides an indoor propagation method for hornworts horneri, which specifically comprises the following steps:
(1) taking pupa of Marila abrupt-chest cocoon bee to breed indoors, and taking the adult parasitic bee on the emergence day 3 as a seed bee after the parasitic bee emerges; indoor feeding temperature is 25 +/-1 ℃, relative humidity is 70-80%, and photoperiod is L: D-14: 10 h;
(2) taking a centrifuge tube which is provided with 1ml of cover pricked holes and connected with 1 head of 1-3-instar larvae of the host asparagus caterpillars, inoculating 1 head of each of the 3d parasitic wasp male and female adults to eclosion, then placing the centrifuge tube in an artificial climate chamber to carry out co-feeding of the parasitic wasps and the hosts, changing the hosts once every 24h until all the female parasitic wasps die, and continuously changing new hosts for 9 times in total; the conditions in the climatic chamber are: the temperature is 25 +/-1 ℃, the relative humidity is 75-80%, and the photoperiod is L: d is 14:10 h;
(3) after the parasitic wasps and the hosts are bred for 24 hours, taking out the parasitic wasps, re-inoculating the parasitic wasps into a new centrifugal tube connected with the spodoptera exigua 2-instar larvae, repeating the steps until all the female parasitic wasps die, and counting the parasitic success rate; the success rate (%) of parasitization was equal to the number of successfully parasitized spodoptera exigua/number of hosts (total number of spodoptera exigua used in the experiment) × 100%;
(4) collecting parasitic wasps which grow to pupal stage from cocoons after being parasitized, sealing the opening of a guide tube (polypropylene is 24 multiplied by 95mm), continuously culturing in an artificial climate chamber, counting the eclosion number after the parasitic wasps eclosion, and calculating the eclosion rate (%) (the eclosion rate is the number of the parasitic wasps eclosion in the parasitic pupa/the total number of the parasitic wasps eclosion and non-eclosion in the parasitic pupa multiplied by 100 percent); feeding with cotton ball containing 20% honey water for nutrition, and eclosion parasitic wasp of 3d for breeding parasitic wasp of next generation.
Example 2
This example differs from example 1 only in that: in step (2), new hosts were continuously replaced 10 times in total.
Example 3
This example differs from example 1 only in that: the adult parasitic wasps of 3d were emerged as the breeding bees, and the rest was exactly the same as in example 1.
Example 4
The embodiment provides an indoor propagation method for hornworts horneri, which specifically comprises the following steps:
(1) taking pupa of Marira abrupt-chest cocoon bee to breed indoors, and taking the imago of parasitic wasp on the 3 rd day after the parasitic wasp is eclosion as a first generation of breeding wasp; indoor feeding temperature is 25 +/-1 ℃, relative humidity is 75-80%, and photoperiod is L: D-14: 10 h;
(2) inoculating the first generation of bee species in the step (1) into an insect breeding cage, then putting a turnover box in which pea seedlings are inoculated with 2-instar larvae of host beet armyworms into the insect breeding cage, wherein the number of heads of the host beet armyworms is 50, the number of heads of adult parasitic wasps is 6 respectively for male and female, putting the turnover box in an artificial climate chamber for co-breeding of the parasitic wasps and the hosts, replacing the hosts once every 24 hours until all the female parasitic wasps die, and continuously replacing the new hosts 7 times in total; the conditions in the climatic chamber are: the temperature is 25 +/-1 ℃, the relative humidity is 75-80%, and the photoperiod is L: D-14: 10 h;
the insect-raising cage is structured as follows: the square body is formed by assembling 12 plastic frameworks of 50cm, a nylon gauze of 80 meshes and 4 plastic tee frameworks, and the specification is that the length is 50cm, the width is 50cm and the height is 50 cm;
the turnover box is made of plastic, the length, the width and the height are 32.00cm, 24.30cm, 13.00cm, 1 rectangular basket with the length of 31.80cm, the width of 22.00cm and the height of 8.50cm is placed in the turnover box, pea seedlings are planted in the rectangular basket, water is added into the turnover box to submerge the roots of the pea seedlings, and the rectangular basket and the turnover box are separated and have water, so that climbing of asparagus caterpillars is effectively prevented;
(3) after parasitic wasps and hosts are bred for 24 hours, taking out the turnover box and placing the turnover box in a new insect breeding cage, placing a new pea seedling turnover box connected with the hosts in the original insect breeding cage for continuous parasitic, repeating the steps until all the parasitic wasps of females die, and counting the parasitic success rate; the success rate (%) of parasitization was equal to the number of successfully parasitized spodoptera exigua/number of hosts (total number of spodoptera exigua used in the experiment) × 100%;
(4) collecting parasitic bee cocoons which grow to pupa stage from the parasitic cocoons, sealing the pipe openings in a finger tube (polypropylene is 24 multiplied by 95mm), continuously culturing in an artificial climate chamber, counting the eclosion number after the parasitic bee cocoons emerge, and calculating the eclosion rate (%) (the eclosion rate is the number of the parasitic bees emerging in the parasitic pupa/the total number of the parasitic bees emerging and the parasitic bees which do not emerge in the parasitic pupa multiplied by 100%; feeding cotton balls containing 10% sucrose solution for nutrition, and using the 3 rd parasitic wasp for breeding the next generation parasitic wasp.
Example 5
This example differs from example 4 only in that in step (2), the new host was continuously replaced 8 times in total, and the rest was exactly the same as example 4.
Example 6
The difference between the embodiment and the embodiment 5 is only that the circulation box is made of plastic, the length, the width and the height are 32.00cm, 24.30cm, 13.00cm, the circulation box is internally provided with nutrition formula soil, the nutrition formula soil comprises 4.00kg of soil, 80.00g of perlite and 110.00g of vermiculite, 150 peas soaked for 1 day are planted in the nutrition formula soil, and 1-3-year asparagus caterpillars are inoculated after the pea seedlings grow out. The rest was exactly the same as in example 5.
Example 7
This example differs from example 4 only in that in step (2) the adult parasitic wasp has a hermaphroditic ratio of 8: 1.
Example 8
This example differs from example 4 only in that in step (2), the ratio of the number of heads of the host spodoptera exigua larvae to the number of heads of the parasitic wasp female adults was 100:1 before co-rearing.
Example 9
The difference between this example and example 4 is only that in step (3), the parasitic wasp and the host are co-bred for 12 hours, and the host is replaced every 12 hours.
Example 10
The difference between this example and example 4 is only that in step (3), the parasitic wasp and the host are co-bred for 48 hours, and the host is replaced every 48 hours.
In examples 1 to 6 described above, the statistical success rate of parasitism and the feathering rate are shown in Table 1.
TABLE 1 statistics of parasitic success and emergence rates of hornet bees
As can be seen from the table 1, the invention takes the 1-3 instar larvae of the spodoptera exigua as hosts, the hornet of the Marina marela can parasitize in a large amount and generate a large amount of offspring, and can be stored at low temperature and normal temperature in a pupa form, thereby realizing the indoor large-scale propagation of the hornet of the Marela marela, providing an insect source for the field release application of the hornet, and providing technical support for the biological control of noctuidae pests.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.
Claims (10)
1. An indoor propagation method for hornworts cunea kuntze is characterized by comprising the following steps:
(1) pupae of the Marira abrupt-chest cocoons are taken to be bred indoors, and the pupae are taken as first generation of breeding bees after parasitic bees emerge;
(2) inoculating the first generation of bee in the step (1) into an insect breeding cage, then putting a turnover box connected with host spodoptera exigua larvae into the insect breeding cage, and putting the turnover box into an artificial climate chamber for co-breeding parasitic bees and hosts; the first generation of breeding bees include adult female parasitic bees and adult male parasitic bees;
(3) after parasitic wasps and hosts are co-bred for a period of time, taking out the turnover box and placing the turnover box in a new insect breeding cage, replacing the new hosts with the original insect breeding cage for continuous parasitism, replacing the hosts once every a period of time, and repeating the steps until all the parasitic wasps of females die;
(4) collecting parasitic wasp cocoons which grow to pupal stage after parasitization, placing the parasitic wasps in an artificial climate chamber for culturing until emergence, and using the parasitic wasps after emergence as seed wasps for breeding parasitic wasps of the next generation.
2. The indoor propagation method for the hornworts cunea as claimed in claim 1, wherein in the step (1), the indoor breeding temperature is 25 ± 1 ℃, the relative humidity is 75-80%, and the photoperiod is L: D: 14:10 h.
3. The indoor propagation method for the hornet bombycis as claimed in claim 1, wherein in step (2), the first generation bees are adult parasitic bees which emerge for 2-3d, and the ratio of male to female of the adult parasitic bees is 1:1-8: 1.
4. The indoor propagation method of hornworts margarita as claimed in claim 1, wherein in step (2), the host beet armyworm larvae are 1-3 instar larvae of the host beet armyworm.
5. The indoor propagation method of hornworts margarita as claimed in claim 1, wherein in the step (2), when co-rearing is carried out, the ratio of the number of heads of the host spodoptera exigua larvae to the number of heads of the parasitic wasp female adults is 1:1-100: 1.
6. The indoor propagation method for hornworts with steep thoracons of manila as claimed in claim 1, wherein in step (2), the conditions in the climatic chamber are: the temperature is 25 +/-1 ℃, the relative humidity is 75-80%, and the photoperiod is L: D-14: 10 h.
7. The indoor propagation method of hornet bombycis brevifiliformis according to claim 1, wherein in the step (3), the parasitic wasp and the host are co-bred for 12-48h, and the host is replaced every 12-48 h.
8. The indoor propagation method of hornworts with steep thorns as claimed in claim 1, wherein in step (4), the parasitic wasps cocoons which grow after parasitizing to pupal stage are collected in the fingered tube, the tube opening is sealed with a sponge plug, the cultivation is continued in an artificial climate chamber, after emergence, the parasitized wasps are fed with a cotton ball containing 20% of honey water or 10% of sucrose water for nutrition, and the parasitized wasps of 3d of emergence are used for propagation of the next generation parasitic wasps.
9. The indoor propagation method for the Marira steep chest calluses as claimed in claim 1, wherein the turnover box is made of plastic, the length, width and height of the turnover box are 32.00cm, 24.30cm, 13.00cm, 1 rectangular basket with the length of 31.80cm, the width of 22.00cm and the height of 8.50cm is placed in the turnover box, the rectangular basket is fully planted with pea seedlings and is inoculated with larvae of 1-3 instar of the host beet armyworms, the pea seedlings are submerged by adding water into the turnover box, the rectangular basket is spaced from the turnover box and has water, and therefore climbing of the beet armyworms is effectively prevented and controlled.
10. The indoor propagation method of the hornet masoni steep chest cocoon bee as claimed in claim 1, wherein the turnover box is made of plastic, the length, the width and the height are 32.00cm, the width and the height are 24.30cm, the height is 13.00cm, the turnover box is filled with nutrition formula soil, the nutrition formula soil comprises 4.00kg of peat soil, 80.00g of perlite and 110.00g of vermiculite, 150 peas soaked for 1d are planted in the nutrition formula soil, and after the peas grow out, 1-3-year asparagus caterpillars are inoculated.
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| CN115067441A (en) * | 2022-06-15 | 2022-09-20 | 湖北省生物农药工程研究中心 | Lepidoptera noctuidae larva feed for parasitic wasp propagation and preparation method thereof |
Citations (9)
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