CN113803924B - Cell refrigerator capable of preventing cell pollution - Google Patents

Cell refrigerator capable of preventing cell pollution Download PDF

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Publication number
CN113803924B
CN113803924B CN202111090722.7A CN202111090722A CN113803924B CN 113803924 B CN113803924 B CN 113803924B CN 202111090722 A CN202111090722 A CN 202111090722A CN 113803924 B CN113803924 B CN 113803924B
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China
Prior art keywords
refrigerator
liquid nitrogen
shell
fixedly connected
close
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CN202111090722.7A
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Chinese (zh)
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CN113803924A (en
Inventor
赵宝玉
陈凯
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Shandong Zhongke Saiao Biotechnology Co ltd
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Shandong Zhongke Saiao Biotechnology Co ltd
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    • FMECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25DREFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
    • F25D3/00Devices using other cold materials; Devices using cold-storage bodies
    • F25D3/10Devices using other cold materials; Devices using cold-storage bodies using liquefied gases, e.g. liquid air
    • FMECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25DREFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
    • F25D23/00General constructional features
    • FMECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25DREFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
    • F25D25/00Charging, supporting, and discharging the articles to be cooled
    • F25D25/02Charging, supporting, and discharging the articles to be cooled by shelves
    • F25D25/024Slidable shelves
    • FMECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F25REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
    • F25DREFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
    • F25D29/00Arrangement or mounting of control or safety devices
    • F25D29/008Alarm devices

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  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Combustion & Propulsion (AREA)
  • Physics & Mathematics (AREA)
  • Mechanical Engineering (AREA)
  • Thermal Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Devices That Are Associated With Refrigeration Equipment (AREA)

Abstract

The utility model relates to the technical field of refrigerators, in particular to a cell refrigerator capable of preventing cell pollution, which comprises a liquid nitrogen tank, wherein a pressure pump is fixedly connected to the position, close to the lower end, of the annular outer surface of the liquid nitrogen tank, a transfusion tube is fixedly arranged at the position, close to the other side of the lower end, of the annular outer surface of the liquid nitrogen tank, one end of the transfusion tube is fixedly connected with a refrigerator main body mechanism, the right side of the refrigerator main body mechanism is fixedly connected with an operation box shell, two operation holes are symmetrically formed in the position, close to the right side, of the outer surface of the upper end of the operation box shell, and a first box door is rotatably connected to the middle position of the outer surface of the upper end of the operation box shell. According to the utility model, automatic liquid nitrogen addition can be completed, liquid nitrogen in the liquid nitrogen tank can be automatically injected into the liquid nitrogen cavity, manual regular disassembly and addition are not needed, the problem that liquid nitrogen consumption is aggravated due to poor tightness caused by regular disassembly is avoided, the cost is saved, the time and the labor are saved, and the method is convenient and quick.

Description

Cell refrigerator capable of preventing cell pollution
Technical Field
The utility model relates to the technical field of refrigerators, in particular to a cell refrigerator capable of preventing cell pollution.
Background
Cells are fundamental units of biological structure and physiological function, and are the basis of growth and development. The cell morphology is various, and there are sphere, square, column, etc. The size of the cells varies greatly, most of the cells have diameters of only a few microns, and some can reach more than 100 microns. Although the cell forms and sizes are different, the structure is basically the same, all cells except mature red blood cells and platelets have at least one cell nucleus, which is a control center for regulating vital movement, controlling division, differentiation, inheritance and variation of the cells, the prior art uses liquid nitrogen for refrigerating the cells, the temperature of the liquid nitrogen is below 147 ℃ below zero under normal pressure, and a large amount of heat is absorbed when the liquid nitrogen is gasified into nitrogen, so that the liquid nitrogen can be cooled and refrigerated well, and the nitrogen is one of main components of air and is easy to obtain.
The Chinese patent discloses a stem cell fridge, and patent application number is 201821227597.3, and a stem cell fridge includes the box, set up the inner bag in the box, set up frozen bottle in the inner bag, frozen bottle passes through bottle closing cap portion and sets up inside the inner bag, bottle closing cap portion erects in the box top, set up non-contact thermometer in the inner bag, place liquid nitrogen between the space of inner bag and box. The stem cell refrigerator provided by the utility model has the advantages that the structure is simple, the storage and the taking are convenient, the stem cell transfer bag in the storage box can obtain a good refrigeration and moisture preservation effect, and the stem cell transfer bag is doubly protected. The box body and the box door are composed of an inner layer, an outer layer and a middle heat insulation layer, so that the heat insulation effect is good, and the nitrogen loss is reduced.
The above-mentioned contrast patent technique is simple in structure, convenient access, can obtain fine cold-stored moisturizing effect, divide into a plurality of cold-stored rooms with the box, can deposit simultaneously and freeze the pipe and freeze the bag, satisfy different demands, and every cold-stored room all installs temperature sensor, temperature sensor can real-time supervision each cold-stored room's freezing condition, but prior art sets up the device of replenishing liquid nitrogen, after the liquid nitrogen uses, just need dismantle the mechanism device between box and inner bag, then add the liquid nitrogen into the space between inner bag and the box, install the seal again afterwards, the liquid nitrogen needs periodic interpolation to supplement, it is more troublesome when dismantling, long-time dismantlement installation also can lead to the leakproofness to become poor, the consumption that aggravates liquid nitrogen has increased the cost, consuming time and effort, the practicality is unable in time confirm whether the liquid nitrogen between inner bag and the box space has consumed, unable timely interpolation liquid nitrogen can lead to the temperature in the cold-stored box to rise, the cold-stored effect is poor.
To this end, a cell freezer is proposed which prevents contamination of cells.
Disclosure of Invention
The present utility model is directed to a cell refrigerator capable of preventing cell contamination, and is intended to solve the above-mentioned problems of the prior art.
In order to achieve the above purpose, the present utility model provides the following technical solutions: the utility model provides a prevent cell fridge of cell pollution, includes the liquid nitrogen jar, the annular surface of liquid nitrogen jar is close to the position fixedly connected with force pump of lower extreme one side, the annular surface of liquid nitrogen jar is close to the position fixedly connected with transfer line of lower extreme opposite side, the one end fixedly connected with fridge main part mechanism of transfer line, the right side fixedly connected with control box casing of fridge main part mechanism, two operation holes have been seted up to the upper end surface symmetry that is close to the right side of control box casing, the upper end surface intermediate position rotation of control box casing is connected with first chamber door, the right side surface upper end of control box casing is connected with the second chamber door through the hinge rotation, the position fixedly connected with arc handle of the right side surface upper end edge of second chamber door, the internal surface lower extreme fixedly connected with culture dish of control box casing places the dish, the position fixedly connected with ultraviolet irradiation lamp that the internal surface upper end of control box casing is close to the centre.
The main body mechanism of the refrigerator comprises a refrigerator shell, a liquid nitrogen cavity, a controller tube, a refrigerator liner, an automatic ejection mechanism, a third refrigerator door, a refrigerator door handle, an alarm mechanism, an electromagnetic valve closing controller, a hollow metal ball and an electromagnetic valve opening controller; the left side of control box casing sets up to the opening, the right side of fridge casing and the left side fixed connection of control box casing, the vertical fixed mounting of inner surface left end near border position of fridge casing has the controller pipe, the fixed mounting of inner surface upper end of controller pipe has the solenoid valve to close the controller, the fixed mounting of inner surface lower extreme of controller pipe has the solenoid valve to open the controller, be provided with hollow metal ball in the controller pipe passageway between solenoid valve to close the controller and the solenoid valve to open the controller, the fixed mounting of inner surface right-hand member of fridge casing has the fridge inner bag, the liquid nitrogen chamber has been seted up between fridge casing and the fridge inner bag, the opening has been seted up to the right-hand member of fridge inner bag, and the opening inner upper end rotates and install the third chamber door, the right-hand member surface of third chamber door is close to border position fixed mounting has the chamber door handle, the inside intermediate position sliding connection of fridge inner bag has automatic ejection mechanism, the fixed mounting of lower extreme central point of fridge casing has alarm mechanism, the fixed mounting of outer disc of infusion pipe is close to the fridge casing.
The prior art is that liquid nitrogen is injected into a gap between the inner container and the box body to cool through liquid nitrogen, when the liquid nitrogen needs to be added, a mechanism device between the inner container and the box body needs to be disassembled firstly, then the liquid nitrogen is injected into the gap between the inner container and the box body, then the device is installed and sealed, the liquid nitrogen needs to be regularly disassembled to be added, the gap between the inner container and the box body is ensured to be cooled and refrigerated, the refrigerating effect is ensured, when the liquid nitrogen is added, the mechanism device between the inner container and the box body needs to be disassembled, the disassembly is troublesome, the sealability is poor due to long-time disassembly and installation, the consumption of the liquid nitrogen is aggravated, and the cost is increased; when the electromagnetic valve opening controller is opened, the electromagnetic valve is opened, the pressure pump continuously sends liquid nitrogen into the liquid nitrogen cavity from the liquid nitrogen tank through the infusion tube, the liquid level of the liquid nitrogen continuously rises, the hollow metal ball in the controller tube also continuously rises along with the rising of the liquid level, and when the liquid level of the liquid nitrogen rises to be close to the electromagnetic valve closing controller,the hollow metal ball touches the electromagnetic valve closing controller, the electromagnetic valve is closed, liquid nitrogen is stopped to be input, liquid nitrogen is added, when the liquid nitrogen in the liquid nitrogen tank is about to be consumed, the electromagnetic valve is powered off, then the liquid nitrogen tank is disassembled, liquid nitrogen is injected, then the electromagnetic valve is installed, and then the electromagnetic valve is powered on, wherein the density of the liquid nitrogen is 0.81g/cm 3 The electromagnetic valve opening controller and the electromagnetic valve closing controller are capacitive point type touch switch modules based on touch detection IC (TTP 223B), the automatic liquid nitrogen adding is completed by arranging the main body mechanism of the refrigerator, when the liquid nitrogen in the liquid nitrogen cavity is consumed to a certain extent, the liquid nitrogen in the liquid nitrogen tank can be automatically injected into the liquid nitrogen cavity, the manual regular disassembly and adding are not needed, the problem that the liquid nitrogen is consumed due to the worsening of tightness caused by regular disassembly is avoided, the cost is saved, the time and the labor are saved, and the refrigerator is convenient and quick.
Preferably, the automatic ejection mechanism comprises a test tube rack, an electric telescopic rod, a movable sliding block, a sliding chute, a buffer mechanism and a test tube placing hole; two spouts have been seted up to the both ends symmetry of fridge inner bag, two the equal fixed mounting of left end of spout has electric telescopic handle, electric telescopic handle's right-hand member fixed mounting has the removal slider, the one end and the test-tube rack fixed connection of removal slider, test tube placing hole has evenly been seted up to the upside surface and the downside surface of test-tube rack, the right-hand member of spout is provided with buffer gear.
When the cell in the refrigerator needs to be taken out, a worker wears the protective glove, stretches into the refrigerator to take out the test tube on the test tube rack, pours the cells in the test tube into the culture dish in the operation box, then puts the test tube back into the refrigerator, the temperature in the refrigerator is very low, the worker wears the protective glove to prevent own hands from being frozen or frostbite, but the worker wears the protective glove to cause inconvenient operation, errors are easily caused, the utility model conveniently and rapidly takes out and puts back the test tube by arranging the automatic ejection mechanism, when the test tube in the refrigerator needs to be taken out, the third box door is opened, the electric telescopic rod is started, the electric telescopic rod drives the movable slide block to move rightwards, the movable slide block drives the test tube rack connected with the movable slide block to move rightwards, when the movable slide block moves to the right end of the sliding groove, the buffer mechanism is contacted, then slowly moves rightwards, at the position close to the rightmost end of the sliding groove, at the moment, the test tube rack is moved into the operation box, then the test tube rack is poured into the culture dish, then the test tube is put back into the electric slide block, the electric telescopic rod is started, the electric telescopic rod is driven to move leftwards, and finally the cell telescopic rod is driven to move leftwards, the test tube rack is completely to be closed, and the cell telescopic rod is completely moved, and the test tube is completely is moved to be closed; the automatic pop-up of test tube rack is accomplished through setting up automatic pop-up mechanism, when the staff need take out the cell in the test tube, need not wear heavy protective glove again and stretch into in the fridge and take out and put back the test tube, only need start electric telescopic handle just can pop-up the test tube rack in the fridge, can convenient and fast take out and put back the test tube, convenient and fast more has avoided wearing the mistake that the gloves probably caused, has also prevented that the staff's hand from being frozen stiff or frostbite.
Preferably, the buffer mechanism comprises a buffer gasket and a buffer spring; the buffer mechanism is arranged in the chute, the inner surface of the inner container of the refrigerator at one end of the buffer spring is fixedly connected, and the buffer gasket is fixedly connected at the other end of the buffer spring.
When the test tube is taken out during operation, the automatic ejection mechanism moves rightwards, when the test tube moves to the rightmost end of the chute, the movable sliding block possibly collides with the inner wall of the chute directly, after long-time use, the abrasion and damage of the automatic ejection mechanism can be caused, and the service life of the automatic ejection mechanism is reduced.
Preferably, the alarm mechanism comprises an alarm shell, a compression spring, a hollow shell, a conductive sheet, a closed baffle, a buzzer and a sealing piston; the alarm shell is fixedly connected with the lower end of the inner surface of the refrigerator shell, and two compression springs are symmetrically arranged at the edge of the top end of the inner surface of the alarm shell; the lower ends of the two compression springs are fixedly connected with hollow shells, the inner surfaces of the alarm shells are close to the middle positions and fixedly connected with conducting strips, the positions, close to the lower ends, of the inner surfaces of the alarm shells are fixedly connected with airtight baffles, through holes are formed in the lower ends of the airtight baffles, buzzers are fixedly connected to the outer surfaces of the lower ends of the airtight baffles, and sealing pistons are arranged at the lower ends of the buzzers.
When the electromagnetic valve and the pressure pump cannot work normally, so that timely liquid nitrogen replenishment cannot be performed in the liquid nitrogen cavity, workers cannot find timely, liquid nitrogen in the liquid nitrogen cavity is completely consumed, the temperature in the refrigerator is increased, and the refrigerating effect is poor.
Preferably, a sealing piston is arranged at the lower end of the buzzer, and the sealing piston is in threaded connection with the refrigerator shell.
When the buzzer is damaged and can not work normally, the buzzer needs to be disassembled and replaced, and the sealing piston is screwed to disassemble the sealing piston and replace the buzzer when the buzzer is damaged.
Preferably, the outer surface of the refrigerator shell is nested with an insulating layer for enhancing the refrigerating effect, and the insulating layer is a member made of glass fiber insulating cotton.
When the refrigerator is used for refrigerating cells, the liquid nitrogen continuously gasifies and absorbs heat to ensure the refrigerating effect, the refrigerator is characterized in that the insulating layer is nested outside the refrigerator shell to insulate and strengthen the refrigerating effect, the glass fiber insulating cotton is good in heat insulation, is an excellent heat insulation material, is made of glass, has quite high insulativity, has quite strong corrosion resistance, can not reduce the functional characteristics even if being used for a long time, is good in recovery performance, is resistant to impact vibration, has a moisture absorption rate close to zero under normal conditions, and does not worry about the occurrence of wet cracking caused by water absorption expansion.
Compared with the prior art, the utility model has the beneficial effects that:
1. according to the utility model, the main body mechanism of the refrigerator is arranged to finish automatic liquid nitrogen addition, when liquid nitrogen in the liquid nitrogen cavity is consumed to a certain extent, the liquid nitrogen in the liquid nitrogen tank can be automatically injected into the liquid nitrogen cavity, manual regular disassembly and addition are not needed, the problem that the liquid nitrogen consumption is aggravated due to poor tightness caused by regular disassembly is avoided, the cost is saved, the time and the labor are saved, and the refrigerator is convenient and quick.
2. According to the utility model, the automatic ejection mechanism is arranged to finish the automatic ejection of the test tube rack, when a worker needs to take out cells in the test tube, the worker does not need to wear thick protective gloves to stretch into the refrigerator for taking out and putting back the test tube, and the test tube rack can be ejected from the refrigerator only by starting the electric telescopic rod, so that the test tube can be conveniently and rapidly taken out and put back, the test tube is more convenient and rapid, errors possibly caused by wearing the gloves are avoided, and the worker is prevented from being frozen or frostbite; when the electromagnetic valve and the pressure pump cannot work normally, a worker can find out in time to replace and maintain, and the refrigerating effect of the refrigerator cannot be affected.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present utility model;
FIG. 2 is an overall cross-sectional view of the present utility model;
fig. 3 is a structural view of the refrigerator of the present utility model;
FIG. 4 is a cross-sectional view at A-A of the present utility model;
FIG. 5 is a top cross-sectional view of the automatic pop-up mechanism of the present utility model;
fig. 6 is a view of the structure of the test tube rack of the present utility model;
FIG. 7 is an enlarged view of the cushioning mechanism of the present utility model;
FIG. 8 is an enlarged structural view of the alarm mechanism of the present utility model;
fig. 9 is a partial cross-sectional view of the alert mechanism of the present utility model.
In the figure: 1. a liquid nitrogen tank; 2. a pressure pump; 3. an infusion tube; 4. a refrigerator main body mechanism; 41. a refrigerator case; 42. a liquid nitrogen chamber; 43. a controller tube; 44. a refrigerator liner; 45. an automatic ejection mechanism; 451. a test tube rack; 452. an electric telescopic rod; 453. moving the slide block; 454. a chute; 455. a buffer mechanism; 4551. a buffer pad; 4552. a buffer spring; 456. a test tube placement hole; 46. a third door; 47. a door handle; 48. an alarm mechanism; 481. an alarm housing; 482. a compression spring; 483. a hollow housing; 484. a conductive sheet; 485. a closed baffle; 486. a buzzer; 487. a sealing piston; 49. an electromagnetic valve; 410. a solenoid valve closing controller; 411. hollow metal balls; 412. a solenoid valve opening controller; 5. an operation box housing; 6. a first door; 7. an operation hole; 8. a second door; 9. an arc-shaped handle; 10. a culture dish placing plate; 11. an ultraviolet irradiation lamp; 12. and a heat preservation layer.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
In the description of the present utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present utility model.
Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more of the described features. In the description of the present utility model, the meaning of "a plurality" is two or more, unless explicitly defined otherwise. Furthermore, the terms "mounted," "connected," "coupled," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.
Referring to fig. 1 to 7, the present utility model provides a technical solution for a cell refrigerator for preventing cell contamination:
the utility model provides a prevent cell fridge of cell pollution, is shown in fig. 1 through 4, includes liquid nitrogen jar 1, the annular surface of liquid nitrogen jar 1 is close to the position fixedly connected with pressure pump 2 of lower extreme one side, the annular surface of liquid nitrogen jar 1 is close to the position fixedly connected with transfer line 3 of lower extreme opposite side, the one end fixedly connected with fridge main part mechanism 4 of transfer line 3, the right side fixedly connected with control box casing 5 of fridge main part mechanism 4, two operation holes 7 have been seted up to the upper end surface symmetry that is close to the right side of control box casing 5, the upper end surface intermediate position rotation of control box casing 5 is connected with first chamber door 6, the right side surface upper end of control box casing 5 is connected with second chamber door 8 through the hinge rotation, the position fixedly connected with arc handle 9 of the right side surface upper end edge of second chamber door 8, the internal surface lower extreme fixedly connected with culture dish placement plate 10 of control box casing 5, the position that the internal surface upper end is close to the centre of control box casing 5 is fixedly installed ultraviolet lamp 11.
The main body mechanism 4 of the refrigerator comprises a refrigerator shell 41, a liquid nitrogen cavity 42, a controller tube 43, a refrigerator liner 44, an automatic ejection mechanism 45, a third refrigerator door 46, a refrigerator door handle 47, an alarm mechanism 48, an electromagnetic valve 49, an electromagnetic valve closing controller 410, a hollow metal ball 411 and an electromagnetic valve opening controller 412; the left side surface of the operation box shell 5 is provided with an opening, the right side of the refrigerator shell 41 is fixedly connected with the left side of the operation box shell 5, a controller tube 43 is vertically and fixedly arranged at the position, close to the edge, of the left end of the inner surface of the refrigerator shell 41, an electromagnetic valve closing controller 410 is fixedly arranged at the upper end of the inner surface of the controller tube 43, an electromagnetic valve opening controller 412 is fixedly arranged at the lower end of the inner surface of the controller tube 43, a hollow metal ball 411 is arranged in a controller tube 43 channel between the electromagnetic valve closing controller 410 and the electromagnetic valve opening controller 412, the refrigerator comprises a refrigerator shell 41, a refrigerator liner 44 is fixedly arranged at the right end of the inner surface of the refrigerator shell 41, a liquid nitrogen cavity 42 is formed between the refrigerator shell 41 and the refrigerator liner 44, an opening is formed at the right end of the refrigerator liner 44, a third box door 46 is rotatably arranged at the upper end in the opening, a box door handle 47 is fixedly arranged at the outer surface of the right end of the third box door 46 close to the edge, an automatic ejection mechanism 45 is slidably connected at the middle position inside the refrigerator liner 44, an alarm mechanism 48 is fixedly arranged at the center position of the lower end of the refrigerator shell 41, and an electromagnetic valve 49 is fixedly arranged at the position, close to the refrigerator shell 41, of the outer circular surface of the infusion tube 3.
The prior art is that liquid nitrogen is injected into a gap between the inner container and the box body to cool through liquid nitrogen, when the liquid nitrogen needs to be added, a mechanism device between the inner container and the box body needs to be disassembled firstly, then the liquid nitrogen is injected into the gap between the inner container and the box body, then the device is installed and sealed, the liquid nitrogen needs to be regularly disassembled to be added, the gap between the inner container and the box body is ensured to be cooled and refrigerated, the refrigerating effect is ensured, when the liquid nitrogen is added, the mechanism device between the inner container and the box body needs to be disassembled, the disassembly is troublesome, the sealability is poor due to long-time disassembly and installation, the consumption of the liquid nitrogen is aggravated, and the cost is increased; in operation, liquid nitrogen in the liquid nitrogen cavity 42 is continuously consumed in operation, liquid nitrogen continuously drops in the liquid nitrogen cavity 42, the liquid level of the liquid nitrogen in the liquid nitrogen cavity 42 also continuously drops, the hollow metal ball 411 in the controller tube 43 floats on the liquid level of the liquid nitrogen, the hollow metal ball 411 continuously drops along with the liquid level, when the liquid level of the liquid nitrogen drops to the electromagnetic valve opening controller 412, the hollow metal ball 411 touches the electromagnetic valve opening controller 412 at this time, the electromagnetic valve 49 is opened, the pressure pump 2 continuously feeds the liquid nitrogen into the liquid nitrogen cavity 42 from the liquid nitrogen tank 1 through the liquid conveying pipe 3, the liquid level of the liquid nitrogen continuously rises, the hollow metal ball 411 in the controller tube 43 also continuously rises along with the rising of the liquid level, when the liquid level rises to be close to the electromagnetic valve closing controller 410, the hollow metal ball 411 touches the electromagnetic valve closing controller 410, the electromagnetic valve 49 is closed, the liquid nitrogen stops inputting, the liquid nitrogen is completely added, when the liquid nitrogen in the liquid nitrogen tank 1 is about to be consumed, the electromagnetic valve 49 is disassembled, the liquid nitrogen is injected into the liquid nitrogen tank 1, the liquid nitrogen is then the liquid nitrogen is electrified, and the electromagnetic valve 49 is installed, wherein the liquid nitrogen density is 0.g/cm is obtained 3 The solenoid valve opening controller 412 and the solenoid valve closing controller 410 are capacitive-type touch switch modules based on the touch detection ICTTP223B,according to the utility model, the main body mechanism 4 of the refrigerator is arranged to finish automatic liquid nitrogen addition, when liquid nitrogen in the liquid nitrogen cavity 42 is consumed to a certain extent, the liquid nitrogen in the liquid nitrogen tank 1 can be automatically injected into the liquid nitrogen cavity 42, manual regular disassembly and addition are not needed, the problem that the liquid nitrogen is consumed due to poor tightness caused by regular disassembly is avoided, the cost is saved, the time and the labor are saved, and the refrigerator is convenient and quick.
As an embodiment of the present utility model, as shown in fig. 5 and 6: the automatic ejection mechanism 45 comprises a test tube rack 451, an electric telescopic rod 452, a moving slide 453, a chute 454, a buffer mechanism 455 and a test tube placing hole 456; two sliding grooves 454 are symmetrically formed in two ends of the inner container 44 of the refrigerator, electric telescopic rods 452 are fixedly arranged at the left ends of the two sliding grooves 454, a movable sliding block 453 is fixedly arranged at the right end of each electric telescopic rod 452, one end of each movable sliding block 453 is fixedly connected with a test tube rack 451, test tube placing holes 456 are uniformly formed in the outer surface of the upper side and the outer surface of the lower side of each test tube rack 451, and a buffer mechanism 455 is arranged at the right end of each sliding groove 454; when the cell in the refrigerator needs to be taken out, a worker wears the protective glove, stretches into the refrigerator to take out the test tube on the test tube rack 451, pours the cells in the test tube into the culture dish in the operation box, then puts the test tube back into the refrigerator, the temperature in the refrigerator is very low, the worker wears the protective glove to prevent the hands of the worker from being frozen or frostbite, but the worker wears the protective glove to cause inconvenient operation, errors are easily caused, the utility model conveniently and rapidly takes out and puts back the test tube by arranging the automatic ejection mechanism 45, when the test tube in the refrigerator needs to be taken out, the third box door 46 is opened, the electric telescopic rod 452 is started, the electric telescopic rod 452 drives the movable slider 453 to move rightwards, the movable slider 453 drives the test tube rack 451 connected with the movable slider 453 to move rightwards, the movable slider 453 contacts the buffer mechanism 455 when the movable slider moves to the right end of the chute 454, finally stops at the position close to the rightmost end of the chute 454, the test tube rack 451 moves into the operation box, then the test tube rack 451 is taken out, the test tube rack is poured into the refrigerator to be easily to cause errors, the third box door 46 is opened, the electric telescopic rod 452 is completely moved to move the test tube is completely, and the test tube is completely closed, and the electric telescopic rod 453 is completely moved to the test tube is completely closed; the automatic ejection of the test tube rack 451 is completed by arranging the automatic ejection mechanism 45, when a worker needs to take out cells in the test tube, the worker does not need to wear thick protective gloves to stretch into the refrigerator for taking out and putting back the test tube, the test tube rack 451 can be ejected from the refrigerator only by starting the electric telescopic rod 452, the test tube can be conveniently and rapidly taken out and put back the test tube, the test tube rack is more convenient and rapid, errors possibly caused by wearing the gloves are avoided, and the worker is prevented from being frozen or frostbitten by hands.
As an embodiment of the present utility model, as shown in fig. 7, the buffer mechanism 455 includes a buffer spacer 4551 and a buffer spring 4552; the buffer spring 4552 is disposed in the chute 454, and one end of the buffer spring 4552 is fixedly connected to the inner surface of the refrigerator liner 44, and the other end is fixedly connected to the buffer pad 4551; when the test tube is taken out, the automatic ejection mechanism 45 moves rightwards, when the test tube moves to the rightmost end of the sliding groove 454, the moving slide 453 possibly collides with the inner wall of the sliding groove 454 directly, after long-time use, the abrasion and damage of the automatic ejection mechanism 45 can be caused, and the service life of the automatic ejection mechanism 45 is reduced.
As an embodiment of the cell refrigerator for preventing cell contamination according to the present utility model, as shown in fig. 8 and 9, the alarm mechanism 48 includes an alarm housing 481, a compression spring 482, a hollow housing 483, a conductive sheet 484, a sealing shutter 485, a buzzer 486, and a sealing piston 487; the alarm shell 481 is fixedly connected with the lower end of the inner surface of the refrigerator shell 41, and two compression springs 482 are symmetrically arranged at the edge of the top end of the inner surface of the alarm shell 481; the lower ends of the two compression springs 482 are fixedly connected with a hollow shell 483, two conductive plates 484 are symmetrically and fixedly connected to the inner surface of the alarm shell 481 close to the middle position, through holes are formed in the inner surface of the alarm shell 481 close to the lower ends of the two conductive plates 484, a sealing baffle 485 is fixedly connected to the inner surface of the alarm shell 481 close to the lower ends, a cavity is formed in the lower end of the sealing baffle 485, a buzzer 486 is fixedly connected to the outer surface of the lower end of the sealing baffle 485, the buzzer 486 is arranged in the cavity, and a sealing piston 487 is arranged at the lower end of the buzzer 486; when the electromagnetic valve 49 and the pressure pump 2 cannot work normally, so that timely liquid nitrogen replenishment cannot be performed in the liquid nitrogen cavity 42, workers cannot find timely, liquid nitrogen in the liquid nitrogen cavity 42 is completely consumed, the temperature in the refrigerator is increased, and the refrigerating effect is poor.
As an embodiment of the present utility model, as shown in fig. 8 and 9, a sealing piston 487 is disposed at the lower end of the buzzer 486, and the sealing piston 487 is screwed with the refrigerator case 41; when the buzzer 486 is damaged and can not work normally, the buzzer 486 is required to be disassembled and replaced, the sealing piston 487 is screwed to disassemble and replace the buzzer 486, when the buzzer 486 is damaged, the sealing piston 487 is disassembled, then the buzzer 486 is replaced, the sealing piston 487 is a stepped piston, gaskets are arranged at the steps of the piston to strengthen sealing, meanwhile, threads are arranged on the outer surface of the piston, which is connected with the refrigerator shell 41, and the connection with the refrigerator shell 41 is completed through the threads, so that the sealing effect is better.
As an embodiment of the present utility model, as shown in fig. 1 to 3, the outer surface of the refrigerator shell 41 is nested with a heat-insulating layer 12 for enhancing the refrigerating effect, and the heat-insulating layer 12 is a member made of glass fiber heat-insulating cotton; when the refrigerator is used for refrigerating cells, the liquid nitrogen continuously gasifies and absorbs heat to ensure the refrigerating effect, the heat preservation and the refrigerating effect are enhanced by embedding the heat preservation layer 12 outside the refrigerator shell 41, the heat preservation layer 12 is made of glass fiber heat preservation cotton, the glass fiber heat preservation cotton has good heat insulation property and is an excellent heat insulation material, the raw material used by the glass fiber cotton is glass, the glass fiber has quite high heat insulation property, the glass fiber has quite strong corrosion resistance, the functional characteristics of the glass fiber are not reduced after long-term use, the recovery property of the glass fiber heat preservation cotton is quite good, the glass fiber is not afraid of impact vibration, the moisture absorption rate of the glass fiber is close to zero under the normal condition, and the situation that the glass fiber is wet and cracked due to water absorption expansion is not worry.
The using method comprises the following steps: when the liquid nitrogen filling device is used, firstly, liquid nitrogen in the liquid nitrogen cavity 42 is continuously consumed during normal operation, the liquid level of the liquid nitrogen in the liquid nitrogen cavity 42 is continuously lowered, the hollow metal ball 411 in the controller tube 43 floats on the liquid level of the liquid nitrogen, the hollow metal ball 411 is continuously lowered along with the liquid level of the liquid nitrogen, when the liquid level of the liquid nitrogen is lowered to the electromagnetic valve opening controller 412, the hollow metal ball 411 contacts the electromagnetic valve opening controller 412, the electromagnetic valve 49 is opened, the liquid nitrogen is continuously fed into the liquid nitrogen cavity 42 from the liquid nitrogen tank 1 through the infusion tube 3 by the pressure pump 2, the liquid level of the liquid nitrogen is continuously raised, the hollow metal ball 411 in the controller tube 43 is also continuously raised along with the rise of the liquid level, when the liquid level is raised to be close to the electromagnetic valve closing controller 410, the electromagnetic valve 49 is closed, the liquid nitrogen is stopped from being input, when the liquid nitrogen in the liquid nitrogen tank 1 is about to be consumed, the electromagnetic valve 49 is powered off, then the liquid nitrogen tank 1 is disassembled, the liquid nitrogen is injected into the liquid nitrogen cavity 42, and then the electromagnetic valve 49 is powered on; when the test tube in the refrigerator needs to be taken out, the third box door 46 is opened, the electric telescopic rod 452 is started, the electric telescopic rod 452 drives the moving slide 453 to move rightwards, the moving slide 453 drives the test tube rack 451 connected with the electric telescopic rod 452 to move rightwards, when the moving slide 453 moves to the right end of the sliding groove 454, the buffer spring 4552 is compressed to enable the automatic ejection mechanism 45 to move rightwards slowly, and finally, the automatic ejection mechanism stops at a position close to the rightmost end of the sliding groove 454, at the moment, the test tube rack 451 moves into the operation box, then, the test tube on the test tube rack 451 is taken out and poured into a culture dish, then, the test tube is put back into the test tube rack 451, the electric telescopic rod 452 is started again, the electric telescopic rod 452 drives the moving slide 453 to move leftwards, the test tube rack 451 is driven by the moving slide 453 to move leftwards, the electric telescopic rod 452 is closed, and finally, the third box door 46 is closed, and cell taking and placing are completed; when the electromagnetic valve 49 and the pressure pump 2 cannot work normally, the liquid nitrogen cavity 42 cannot be supplemented with liquid nitrogen timely, so that the liquid nitrogen is continuously consumed, the inner surface of the alarm shell is provided with a through hole close to the lower end of the conducting plate 484, so that the liquid nitrogen in the alarm shell 418 flows with the liquid nitrogen in the liquid nitrogen cavity 42, the liquid level is continuously reduced, the hollow shell 483 is a sealed shell, the inside is filled with air, the whole density of the hollow shell 483 is smaller than that of the liquid nitrogen, when the liquid level is reduced to a certain degree, a part of the hollow shell 483 floats on the liquid level of the liquid nitrogen, at the moment, the compression spring 482 moves the hollow shell 483 downwards through the elastic potential energy obtained by compression, the hollow shell 483 continuously moves downwards along with the continuous reduction of the liquid level, finally the hollow shell 483 contacts the conducting plate 484, the buzzer 486 is connected with a circuit, an alarm sound is sent, when the buzzer 486 is damaged, the sealing piston 487 is screwed, the sealing piston 487 is detached, and then the buzzer 486 is replaced.
The electric elements are all connected with an external main controller and 220V mains supply through a transformer, and the main controller can be conventional known equipment for controlling a computer and the like.
Although embodiments of the present utility model have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the utility model, the scope of which is defined in the appended claims and their equivalents.

Claims (4)

1. Cell fridge for preventing cell pollution, including liquid nitrogen container (1), its characterized in that: the device is characterized in that a pressure pump (2) is fixedly connected to the position, close to one side of the lower end, of the annular outer surface of the liquid nitrogen tank (1), a transfusion tube (3) is fixedly installed at the position, close to the other side of the lower end, of the annular outer surface of the liquid nitrogen tank (1), a refrigerator main body mechanism (4) is fixedly connected to one end of the transfusion tube (3), an operation box shell (5) is fixedly connected to the right side of the refrigerator main body mechanism (4), two operation holes (7) are symmetrically formed in the position, close to the right side, of the outer surface of the upper end of the operation box shell (5), a first box door (6) is rotationally connected to the middle position of the outer surface of the upper end of the operation box shell (5), a second box door (8) is rotationally connected to the upper end of the outer surface of the right side of the operation box shell (5) through a hinge, an arc handle (9) is fixedly connected to the upper edge of the outer surface of the right side of the second box door (8), a culture dish placing plate (10) is fixedly connected to the lower end of the inner surface of the operation box shell (5), and an ultraviolet lamp (11) is fixedly installed at the position, close to the middle position of the upper end of the inner surface of the operation box shell (5).
The refrigerator main body mechanism (4) comprises a refrigerator shell (41), a liquid nitrogen cavity (42), a controller tube (43), a refrigerator liner (44), an automatic ejection mechanism (45), a third refrigerator door (46), a refrigerator door handle (47), an alarm mechanism (48), an electromagnetic valve (49), an electromagnetic valve closing controller (410), a hollow metal ball (411) and an electromagnetic valve opening controller (412); the left side of the operation box shell (5) is provided with an opening, the right side of the refrigerator shell (41) is fixedly connected with the left side of the operation box shell (5), a controller tube (43) is vertically and fixedly arranged at the position, close to the edge, of the left end of the inner surface of the refrigerator shell (41), an electromagnetic valve closing controller (410) is fixedly arranged at the upper end of the inner surface of the controller tube (43), an electromagnetic valve opening controller (412) is fixedly arranged at the lower end of the inner surface of the controller tube (43), a hollow metal ball (411) is arranged in a controller tube (43) channel between the electromagnetic valve closing controller (410) and the electromagnetic valve opening controller (412), a refrigerator liner (44) is fixedly arranged at the right end of the inner surface of the refrigerator shell (41), a liquid nitrogen cavity (42) is arranged between the refrigerator shell (41) and the refrigerator liner (44), an opening is formed at the upper end of the right end of the refrigerator liner (44), a third box door (46) is rotatably arranged at the upper end edge of the opening, an alarm mechanism (48) is fixedly arranged at the lower end, close to the center of the refrigerator liner (41), and the alarm mechanism (45) is fixedly arranged at the lower end of the refrigerator liner (45), an electromagnetic valve (49) is fixedly arranged at the position, close to the refrigerator shell (41), of the outer circular surface of the infusion tube (3);
the automatic ejection mechanism (45) comprises a test tube rack (451), an electric telescopic rod (452), a movable sliding block (453), a sliding chute (454), a buffer mechanism (455) and a test tube placing hole (456); two sliding grooves (454) are symmetrically formed in two ends of the inner container (44) of the refrigerator, electric telescopic rods (452) are fixedly arranged at the left ends of the sliding grooves (454), movable sliding blocks (453) are fixedly arranged at the right ends of the electric telescopic rods (452), one ends of the movable sliding blocks (453) are fixedly connected with test tube racks (451), test tube placing holes (456) are uniformly formed in the upper outer surfaces and the lower outer surfaces of the test tube racks (451), and buffer mechanisms (455) are arranged at the right ends of the sliding grooves (454);
the alarm mechanism (48) comprises an alarm shell (481), a compression spring (482), a hollow shell (483), a conductive sheet (484), a sealing baffle (485), a buzzer (486) and a sealing piston (487); the alarm shell (481) is fixedly connected with the lower end of the inner surface of the refrigerator shell (41), and two compression springs (482) are symmetrically arranged at the edge of the top end of the inner surface of the alarm shell (481); the lower extreme fixedly connected with hollow casing (483) of two compression spring (482), alarm casing (481) internal surface is close to intermediate position fixedly connected with conducting strip (484), the position fixedly connected with airtight baffle (485) that alarm casing (481) internal surface is close to the lower extreme, the through-hole has been seted up to the lower extreme of airtight baffle (485), and the lower extreme surface fixedly connected with bee calling organ (486) of airtight baffle (485), bee calling organ (486) set up in the through-hole.
2. A cell freezer for preventing cell contamination as recited in claim 1, wherein: the buffer mechanism (455) comprises a buffer pad (4551), a buffer spring (4552); the buffer spring (4552) is arranged in the chute (454), one end of the buffer spring (4552) is fixedly connected with the inner surface of the refrigerator liner (44), and the other end of the buffer spring is fixedly connected with the buffer pad (4551).
3. A cell freezer for preventing cell contamination as recited in claim 1, wherein: the lower end of the buzzer (486) is provided with a sealing piston (487), and the sealing piston (487) is in threaded connection with the refrigerator shell (41).
4. A cell freezer for preventing cell contamination as recited in claim 1, wherein: the outer surface of the refrigerator shell (41) is nested with the heat preservation layer (12) for enhancing the refrigerating effect, and the heat preservation layer (12) is a member made of glass fiber heat preservation cotton.
CN202111090722.7A 2021-09-17 2021-09-17 Cell refrigerator capable of preventing cell pollution Active CN113803924B (en)

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