CN113777099A - Quintuplet test for rapidly detecting TORCHIgM antibody - Google Patents
Quintuplet test for rapidly detecting TORCHIgM antibody Download PDFInfo
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- CN113777099A CN113777099A CN202110857308.8A CN202110857308A CN113777099A CN 113777099 A CN113777099 A CN 113777099A CN 202110857308 A CN202110857308 A CN 202110857308A CN 113777099 A CN113777099 A CN 113777099A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
- G01N33/56994—Herpetoviridae, e.g. cytomegalovirus, Epstein-Barr virus
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Abstract
The invention discloses a quintuplet test for rapidly detecting a TORCHIgM antibody, which comprises a card body, wherein the top of the card body is provided with a groove, a detection chip is arranged in the groove and comprises a bottom plate, an absorption pad and a nitrocellulose NC membrane, the absorption pad is fixedly arranged on the surface of the bottom plate, the quintuplet test card has the beneficial effects that only one injection port is designed, the five items of TORCH can be detected at the same time only by adding detected liquid into the injection port, and the result is obtained, because the TORCH detection is carried out by five items at one time, the detection sequence does not exist, the use process of the quintuplet test card is simple and rapid, thereby solving the problems that the existing detection mode needs to be respectively added into five sample adding holes, the whole detection process is complex, the sample adding holes are too small, errors easily occur, and the reaction result time is different, secondly, when all items need to be detected at one time, the operation process is too complicated.
Description
Technical Field
The invention relates to the technical field of medical supplies, in particular to a five-joint detection method for rapidly detecting a TORCHIgM antibody.
Background
TORCH refers to a pathogen that causes congenital intrauterine infection and perinatal infection to cause malformation in perinatal infants, and is an acronym of a group of pathogenic microorganisms, wherein t (toxopama) is toxoplasma, r (rubella virus) is rubella virus, C (cytomegalo virus) is giant cell, and H (herpes virus) is herpes simplex i/ii, which have common characteristics and can cause maternal and infant infection. The primary infection of the pregnant women is easy to occur due to the endocrine change and the immunity reduction, and the latent virus in the bodies of the infected pregnant women is also easy to be activated to cause recurrent infection. When the pregnant women have viremia, the viruses can be transmitted through placenta or birth canal to infect fetuses, cause premature delivery, abortion, dead fetuses or teratogenesis, and cause damage to a plurality of systems and organs of newborns, thereby causing symptoms of intelligence disorder and the like in different degrees. Particularly in the early trimester of pregnancy, the embryo is in the organogenesis phase, where it is infected with a virus, which can destroy cells or inhibit cell division and proliferation. After the organ formation period, the virus is infected, so that the tissue and organ structure can be damaged, the continuous infection can be formed, the toxin can be continuously removed after birth, and the corresponding pathological changes can be caused. The infection of the fixed TORCH affects the population quality, and has an important relation with the healthy birth and the sound care, and the patients with the TORCH syndrome cause the abortion and the dead fetus of the pregnant women, have serious intellectual disability after birth, cannot take care of the life by oneself, and cause great mental and economic burden. About one infant in China is born every year, and on average, one infant is born every hour, which poses great threat to the good prenatal and postnatal care and population quality, so that the infection diagnosis and treatment work of China attracts general attention.
For detection in the current market, the current Chinese patent discloses a five-item combined detection card, which mainly adopts a gold-labeled chromatography test principle, applies a gene recombinant antigen, combines a monoclonal antibody and displays a detection result, and the process is to add detected objects into five sample adding holes respectively, so that a reagent strip is made to react and develop color according to the gold-labeled chromatography test principle and display the detection result.
Disclosure of Invention
In order to solve the above problems, the present invention provides a quintuplet assay for rapidly detecting the torch antibody, and the present invention is realized by the following technical scheme.
The utility model provides a quintuplet of short-term test TORCHIgM antibody is examined, includes the card body, the top of the card body is seted up flutedly, the detection chip has been placed to the inside of recess, it includes bottom plate, absorption pad and cellulose nitrate NC membrane to detect the chip, absorption pad fixed mounting is on the surface of bottom plate, cellulose nitrate NC membrane fixed mounting is on the surface of absorption pad, the top of the card body is provided with the card lid, the viewing aperture has been seted up at the top of card lid, and the inside of viewing aperture inlays and is equipped with observation glass, the injection port has been seted up at observation glass's top, the injection port intercommunication has the reposition of redundant personnel chamber, the other side in reposition of redundant personnel chamber extends to observation glass's below.
Further, the rubber stopper is fixedly installed in the injection port, and the discharge port of the diversion cavity and the detection chip are correspondingly arranged.
Further, the top of the card body is embedded with a sealing strip, and the top of the sealing strip is attached to the bottom of the card cover.
Further, the surface of the card body begins to have a clamping groove, the inner wall fixed mounting of card lid has the fixture block, fixture block and clamping groove joint.
Furthermore, the number of the grooves is five, the number of the detection chips is five, and the surfaces of the five detection chips are respectively provided with a detection area and a contrast area.
Furthermore, toxoplasma gondii, cytomegalovirus, rubella virus, I-type herpes simplex virus and II-type herpes simplex virus recombinant antigens are correspondingly arranged in the detection areas of the five detection chips respectively.
A quintuplet test for rapidly detecting the TORCHIgM antibody comprises the following steps:
preparation before experiment:
s1: and (3) assembling a finished product of the quintuplet IgM antibody rapid detection reagent chip: respectively adding recombinant antigens of toxoplasma, cytomegalovirus, rubella virus, I-type herpes simplex virus and II-type herpes simplex virus to a detection chip, placing the detection chip in a groove in a card body, and then fixing a card cover on the card body through a card slot and a card block to complete the installation of the reagent chip;
s2: and (3) balance treatment: taking out the prepared reagent chip, and standing at room temperature for 30 min;
s3: sample treatment: after the blood is collected by vein by using a serum sample, 3000 r/min is centrifuged for 15min, and the upper serum is sucked for standby.
The operation method comprises the following steps:
s1: moistening the membrane: taking 160uL of washing liquid, and adding the washing liquid on the NC film surface of the cellulose nitrate of the chip through an injection port and a shunt cavity to uniformly wet the film surface;
s2: sample adding: taking 200uL of processed serum to be detected, and adding the processed serum to the NC film surface of the cellulose nitrate;
s3: washing: after the sample is fully infiltrated, in order to ensure the washing effect, the washing is carried out by two steps: adding 120uL of washing liquid on the NC film surface of the cellulose nitrate, and adding 120uL of washing liquid on the NC film surface of the cellulose nitrate after the washing liquid permeates;
s4: color development: after the washing liquid is fully infiltrated, adding a color-developing agent I360uL into the NC film surface of the nitrocellulose;
s5: washing: as with S3;
s6: and (3) detection: and after the washing solution is fully infiltrated, observing whether the color development conditions of the reaction probes and the negative reaction probes are effective, judging the experimental result by naked eyes or a chip reader, wherein the whole operation process is carried out at room temperature, and the detection and analysis are completed within 2 h.
The invention has the advantages that only one injection port is designed, the five items of the TORCH can be detected at the same time only by adding the detected liquid into the injection port, and the result is obtained, because the TORCH detection is carried out for five items at one time, the detection sequence does not exist, the use process of the invention is simple and quick, thereby solving the problems that the existing detection mode needs to add five sample adding holes respectively, the whole detection process is complicated, the sample adding holes are too small, the error is easy to occur, firstly, the reaction time is different, and secondly, the operation process is too complicated when all items need to be detected at one time.
Drawings
In order to more clearly illustrate the technical solution of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained based on these drawings without inventive labor.
FIG. 1: the invention is a schematic diagram of the card body and the card cover in a combined state;
FIG. 2: a top view of the card body of the present invention;
FIG. 3: the card body and the card cover of the invention are in front section view;
FIG. 4: the invention relates to a top section view of a card cover.
The reference numbers are as follows:
1. a card body; 2. a groove; 3. detecting a chip; 31. a base plate; 32. an absorbent pad; 33. a cellulose nitrate NC membrane; 34. a detection zone; 35. a control zone; 4. a cover is clamped; 5. observing glass; 6. an injection port; 7. a shunting cavity; 8. a rubber plug; 9. a sealing strip; 10. a card slot; 11. and (7) clamping blocks.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1-4, a quintuplet of short-term test torch HIgM antibody is examined, including the card body 1, recess 2 has been seted up at the top of the card body 1, detection chip 3 has been placed to recess 2's inside, detection chip 3 includes bottom plate 31, absorption pad 32 and cellulose nitrate NC membrane 33, absorption pad 32 fixed mounting is on the surface of bottom plate 31, cellulose nitrate NC membrane 33 fixed mounting is on the surface of absorption pad 32, the top of the card body 1 is provided with card lid 4, the viewing aperture has been seted up at the top of card lid 4, and the inside of viewing aperture inlays and is equipped with observation glass 5, injection port 6 has been seted up at observation glass 5's top, injection port 6 communicates and has shunted chamber 7, the other side of shunted chamber 7 extends to observation glass 5's below.
Preferably, the rubber stopper 8 is fixedly installed in the injection port 6, the discharge port of the shunt cavity 7 and the detection chip 3 are correspondingly arranged, and the liquid can be prevented from overflowing in the injection process through the arrangement of the rubber stopper 8.
Preferably, the sealing strip 9 is embedded in the top of the card body 1, the top of the sealing strip 9 is attached to the bottom of the card cover 4, and the sealing performance between the card cover 4 and the card body 1 can be improved by the arrangement of the sealing strip 9.
Preferably, the surface of the card body 1 starts to be provided with a clamping groove 10, the inner wall of the card cover 4 is fixedly provided with a clamping block 11, the clamping block 11 is clamped with the clamping groove 10, and the card cover 4 can be conveniently fixed on the card body 1 through the arrangement of the clamping groove 10 and the clamping block 11.
Preferably, the number of the grooves 2 is five, the detection chips 3 are five, and the surfaces of the five detection chips 3 are respectively provided with the detection zone 34 and the control zone 35.
Preferably, the detection regions 34 of the five detection chips 3 are respectively and correspondingly provided with recombinant antigens of toxoplasma gondii, cytomegalovirus, rubella virus, herpes simplex virus type i and herpes simplex virus type ii, and five recombinant antigens are provided, so that five items can be synchronously detected, and errors are avoided.
A quintuplet test for rapidly detecting the TORCHIgM antibody comprises the following steps:
preparation before experiment:
s1: and (3) assembling a finished product of the quintuplet IgM antibody rapid detection reagent chip: respectively adding recombinant antigens of toxoplasma, cytomegalovirus, rubella virus, I-type herpes simplex virus and II-type herpes simplex virus to a detection chip 3, placing the detection chip 3 in a groove 2 in a card body 1, and then fixing a card cover 4 on the card body 1 through a card slot 10 and a card block 11 to complete the installation of the reagent chip;
s2: and (3) balance treatment: taking out the prepared reagent chip, and standing at room temperature for 30 min;
s3: sample treatment: after the blood is collected by vein by using a serum sample, 3000 r/min is centrifuged for 15min, and the upper serum is sucked for standby.
The operation method comprises the following steps:
s1: moistening the membrane: taking 160uL of washing liquid, and adding the washing liquid to the surface 33 of the nitrocellulose NC film of the chip through the injection port 6 and the shunt cavity 7 to uniformly wet the film surface;
s2: sample adding: taking 200uL of processed serum to be detected, and adding the processed serum to the surface of a nitrocellulose NC membrane 33;
s3: washing: after the sample is fully infiltrated, in order to ensure the washing effect, the washing is carried out by two steps: adding 120uL of washing liquid on the surface of the nitrocellulose NC membrane 33, and adding 120uL of washing liquid on the surface of the nitrocellulose NC membrane 33 after the washing liquid permeates;
s4: color development: after the washing liquid is fully infiltrated, adding a color-developing agent I360uL into the surface of the nitrocellulose NC membrane 33;
s5: washing: as with S3;
s6: and (3) detection: and after the washing solution is fully infiltrated, observing whether the color development conditions of the reaction probes and the negative reaction probes are effective, judging the experimental result by naked eyes or a chip reader, wherein the whole operation process is carried out at room temperature, and the detection and analysis are completed within 2 h.
One embodiment of the present invention is:
during the use, get washing liquid 160uL, through injection port 6 and reposition of redundant personnel chamber 7 add in the nitrocellulose NC membrane 33 face of chip on, evenly wet the membrane face, get the treated serum 200uL of examining, add in the nitrocellulose NC membrane 33 face on, treat the sample and fully permeate the back, for guaranteeing the washing effect, divide two steps to wash: adding 120uL of washing liquid on the surface of a nitrocellulose NC membrane 33, after the washing liquid permeates, adding 120uL of washing liquid on the surface of the nitrocellulose NC membrane 33, after the washing liquid permeates sufficiently, taking a color-developing agent I360uL, adding the color-developing agent I33 on the surface of the nitrocellulose NC membrane 33, then washing again, after the washing liquid permeates sufficiently, observing whether the color development conditions of a reaction probe and a negative reaction probe are effective, judging the experiment result by naked eyes or a chip reader, and completing the detection and analysis within 2 h.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise forms disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims (7)
1. A quintuplet test for rapidly detecting TORCHIgM antibody is characterized in that: including the card body (1), recess (2) are seted up at the top of the card body (1), detection chip (3) have been placed to the inside of recess (2), it includes bottom plate (31), absorption pad (32) and cellulose nitrate NC membrane (33) to detect chip (3), absorption pad (32) fixed mounting is on the surface of bottom plate (31), cellulose nitrate NC membrane (33) fixed mounting is on the surface of absorption pad (32), the top of the card body (1) is provided with card lid (4), the viewing aperture has been seted up at the top of card lid (4), and the inside of viewing aperture inlays and is equipped with observation glass (5), injection port (6) have been seted up at the top of observation glass (5), injection port (6) intercommunication has reposition of redundant personnel chamber (7), the other side in reposition of redundant personnel chamber (7) extends to the below of observation glass (5).
2. The quintuplet assay for the rapid detection of TORCHIgM antibodies of claim 1, wherein: the rubber stopper (8) is fixedly installed in the injection port (6), and the discharge port of the diversion cavity (7) is arranged corresponding to the detection chip (3).
3. The quintuplet assay for the rapid detection of TORCHIgM antibodies of claim 1, wherein: the top of the card body (1) is embedded with a sealing strip (9), and the top of the sealing strip (9) is attached to the bottom of the card cover (4).
4. The quintuplet assay for the rapid detection of TORCHIgM antibodies of claim 1, wherein: the surface of the clamp body (1) begins to be provided with a clamping groove (10), the inner wall of the clamp cover (4) is fixedly provided with a clamping block (11), and the clamping block (11) is connected with the clamping groove (10) in a clamping mode.
5. The quintuplet assay for the rapid detection of TORCHIgM antibodies of claim 1, wherein: the quantity of recess (2) is five settings, it is five to detect chip (3), five the surface of detecting chip (3) is provided with detection zone (34) and contrast district (35) respectively.
6. The quintuplet assay for the rapid detection of TORCHIgM antibodies of claim 5, wherein: toxoplasma, cytomegalovirus, rubella virus, I type herpes simplex virus and II type herpes simplex virus recombinant antigens are correspondingly arranged in the detection areas (34) of the five detection chips (3).
7. The quintuplet assay for the rapid detection of torch antibodies according to any one of claims 1 to 6, wherein: the detection method comprises the following steps:
preparation before experiment:
s1: and (3) assembling a finished product of the quintuplet IgM antibody rapid detection reagent chip: toxoplasma, cytomegalovirus, rubella virus, I type herpes simplex virus and II type herpes simplex virus recombinant antigens are respectively added on a detection chip (3), the detection chip (3) is placed in a groove (2) inside a card body (1), and then a card cover (4) is fixed on the card body (1) through a card slot (10) and a card block (11) to complete the installation of the reagent chip;
s2: and (3) balance treatment: taking out the prepared reagent chip, and standing at room temperature for 30 min;
s3: sample treatment: after the blood is collected by vein by using a serum sample, 3000 r/min is centrifuged for 15min, and the upper serum is sucked for standby.
The operation method comprises the following steps:
s1: moistening the membrane: taking 160uL of washing liquid, and adding the washing liquid to the surface of a nitrocellulose NC membrane (33) of the chip through an injection port (6) and a diversion cavity (7) to uniformly wet the membrane surface;
s2: sample adding: taking 200uL of processed serum to be detected, and adding the processed serum to the surface of a nitrocellulose NC membrane (33);
s3: washing: after the sample is fully infiltrated, in order to ensure the washing effect, the washing is carried out by two steps: adding 120uL of washing liquid on the surface of the nitrocellulose NC membrane (33), and adding 120uL of washing liquid on the surface of the nitrocellulose NC membrane (33) after the washing liquid permeates;
s4: color development: after the washing liquid is fully infiltrated, adding a color-developing agent I360uL into the surface of a nitrocellulose NC membrane (33);
s5: washing: as with S3;
s6: and (3) detection: and after the washing solution is fully infiltrated, observing whether the color development conditions of the reaction probes and the negative reaction probes are effective, judging the experimental result by naked eyes or a chip reader, wherein the whole operation process is carried out at room temperature, and the detection and analysis are completed within 2 h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202110857308.8A CN113777099A (en) | 2021-07-28 | 2021-07-28 | Quintuplet test for rapidly detecting TORCHIgM antibody |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101086497A (en) * | 2006-06-09 | 2007-12-12 | 杨致亭 | TORCH five-item combined detection card |
| CN106222081A (en) * | 2016-08-31 | 2016-12-14 | 北京中科圆融生物科技发展有限公司 | Single centre multi-channel assembled detection device |
| CN210294072U (en) * | 2019-04-24 | 2020-04-10 | 杭州微策生物技术有限公司 | Multi-connected urine detection card |
| WO2021134304A1 (en) * | 2019-12-30 | 2021-07-08 | 深圳迈瑞生物医疗电子股份有限公司 | Kit, method and immunoassay analyzer for screening torch infection |
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- 2021-07-28 CN CN202110857308.8A patent/CN113777099A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101086497A (en) * | 2006-06-09 | 2007-12-12 | 杨致亭 | TORCH five-item combined detection card |
| CN106222081A (en) * | 2016-08-31 | 2016-12-14 | 北京中科圆融生物科技发展有限公司 | Single centre multi-channel assembled detection device |
| CN210294072U (en) * | 2019-04-24 | 2020-04-10 | 杭州微策生物技术有限公司 | Multi-connected urine detection card |
| WO2021134304A1 (en) * | 2019-12-30 | 2021-07-08 | 深圳迈瑞生物医疗电子股份有限公司 | Kit, method and immunoassay analyzer for screening torch infection |
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| 吕世静: "临床免疫学检验", 中国医药科技出版社 * |
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