CN113773131A - Preparation and application method of fertilizer synergist - Google Patents
Preparation and application method of fertilizer synergist Download PDFInfo
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- CN113773131A CN113773131A CN202110756680.XA CN202110756680A CN113773131A CN 113773131 A CN113773131 A CN 113773131A CN 202110756680 A CN202110756680 A CN 202110756680A CN 113773131 A CN113773131 A CN 113773131A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
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Abstract
The invention relates to the technical field of fertilizer production, and discloses a preparation method and an application method of a fertilizer synergist. The preparation method comprises the following steps: adding fermentation auxiliary materials such as magnesium sulfate, calcium carbonate, sodium chloride, glucose, soybean protein powder, potassium dihydrogen phosphate, defoaming agent and the like into a fermentation tank, uniformly stirring the raw materials and the fermentation auxiliary materials in the fermentation tank, heating to 121 ℃ for sterilization, adding lactic acid bacteria and photosynthetic bacteria into the sterilized fermentation tank, adding 10kg of 1% nuciferine into the fermentation tank every stirring time, adding 3 times, fermenting for 30 hours, and stopping fermentation when no nuciferine is detected in the fermentation solution. By utilizing the microbial fermentation and conversion process, the extraction efficiency is increased, the molecular spectrum of the effective components is enlarged, the action effect is milder, the synergistic effect is improved, the functional effect of the product is greatly enhanced, the cost of the synergistic raw material is greatly reduced, and the safety and the non-toxicity of the product have higher standard vertebra by adopting the microbial fermentation and conversion process.
Description
Technical Field
The invention relates to the technical field of fertilizer production, in particular to a preparation method and an application method of a fertilizer synergist.
Background
Fertilizer synergist refers to an active substance for the purpose of increasing nutrient availability. The nutrient solution can increase the supply of crop nutrients by retaining nitrogen and activating phosphorus and potassium elements which are difficult to utilize in soil, and plays a certain role in regulating the physiological functions of plants. The fertilizer is usually added into conventional fertilizers, so that the application amount of the fertilizer can be properly reduced, and the utilization rate of the fertilizer is improved. Nuciferine is an aporphine type alkaloid in lotus leaves and is a main lipid-lowering active ingredient in the lotus leaves. Nuciferine has limited effect on plants and also has weak synergistic effect on fertilizers. But after the microbial fermentation treatment, the fermentation liquor has good fertilizer synergistic effect. The nuciferine molecular structure has heterocyclic rings and benzene rings, substances with the heterocyclic rings and the benzene rings with biological activity are formed after decomposition, and many substances with the bis-heterocyclic rings have the biological estrogen effect, such as naphthylacetic acid, salicylic acid, indoleacetic acid, pyraclostrobin and the like, so that the nuciferine molecular structure has the effects of fertilizer synergism and crop growth stimulation.
The microbial fermentation enzyme production is utilized to process related raw materials, the obtained raw materials have good safety and good effect, and besides related target raw materials, other metabolites can be obtained through microbial fermentation, and the products have good effect on crop growth. The common fertilizer synergist is generally produced by chemical industry, and has the disadvantages of poor safety, high cost and insignificant synergism.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a preparation method and an application method of a fertilizer synergist, which have the advantages of saving cost, improving safety, improving the synergist effect and the like, and solve the problems in the background art.
(II) technical scheme
In order to achieve the purposes of saving cost, improving safety and increasing effect, the invention provides the following technical scheme: a fertilizer synergist is prepared by the following steps
1) Preparing raw materials: 600kg of water was added to a 1000L fermenter, and 50kg of dried lotus leaf powder was added.
2) Preparing a fermentation medium: fermenting auxiliary materials such as magnesium sulfate, calcium carbonate, sodium chloride, glucose, soybean protein powder, monopotassium phosphate, defoaming agent and the like according to the proportion of 1: 1: 3: 3: 2: 2: 1 to the fermenter.
3) And (3) sterilization treatment: uniformly stirring the raw materials and the fermentation auxiliary materials in the fermentation tank, heating to 121 ℃ for sterilization, sterilizing for 30 minutes, and then cooling to 35 ℃.
4) Inoculating strains: adding lactobacillus and photosynthetic bacteria into the sterilized fermenter, and controlling the temperature at 28-30 deg.C.
5) And (3) fermentation: and starting stirring for 2 minutes every 3 hours in the fermentation process, starting fermentation for 9 hours, adding 10kg of 1% nuciferine in each stirring time, adding 3 times, fermenting for 30 hours, sampling and detecting nuciferine, qualitatively detecting, and stopping fermentation when no nuciferine is detected in the fermentation solution.
6) Preparing a finished product; removing solid matter and thallus from the fermentation liquid, adding enzyme protective agent into the supernatant, vacuum concentrating at low temperature to obtain liquid lotus leaf carbohydrase liquid preparation, spray drying at low temperature to obtain solid fertilizer synergist
The invention aims to solve another technical problem of providing a preparation method and an application method of a fertilizer synergist, wherein the application method comprises the following steps:
1) preparing a liquid medicine: the fertilizer and the fertilizer synergist required by different crops are mixed and then diluted to prepare the medicinal liquid which can be used.
2) The first use: adding the prepared liquid medicine into a medicine spraying instrument or a medicine dripping instrument, and carrying out primary spraying or drip irrigation on crops according to the growth periods of different crops.
3) And (3) continuous use: the liquid medicine is used once after 10 days, and the using process is consistent with the primary using process.
Preferably, the lotus leaf dry powder is dry leaves of lotus of Nymphaeaceae, collected in sunny days in the early autumn, the collection temperature is controlled at 25-28 ℃, leaf stalks are removed when the lotus leaves are dried to be seventy-eight-turn dry, and the lotus leaves are folded into a semicircle or a fan shape, dried and crushed.
Preferably, the enzyme keeping agent is a combination of maltitol and sorbitol, and the ratio of maltitol to sorbitol is 1: 1.5.
preferably, the nuciferine comprises alkaloids extracted from lotus leaves and derivatives thereof, and the derivatives comprise flavones and polysaccharides.
(III) advantageous effects
Compared with the prior art, the invention provides a preparation method and an application method of a fertilizer synergist, and the fertilizer synergist has the following beneficial effects:
1. the preparation method and the application method of the fertilizer synergist increase the extraction efficiency and expand the molecular spectrum of effective components by utilizing a microbial fermentation and transformation process, have milder action effect, and simultaneously increase the functions of other aspects of the product by beneficial components such as microbial metabolites, mycoprotein and the like, thereby not only improving the synergism, but also greatly enhancing the functional effect of the product. Most importantly, the cost of the synergistic raw materials is greatly reduced while the effect is ensured.
2. The preparation method and the application method of the fertilizer synergist adopt a microbial fermentation and transformation process, so that the product has high safety and no toxicity, has a high standard vertebra, is simple to use, can be used only by dilution, and achieves the purpose of convenient use.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows: a preparation method and an application method of a fertilizer synergist comprise the following steps: preparing raw materials: adding 600kg of water into a 1000L fermentation tank, and then adding 50kg of lotus leaf dry powder; preparing a fermentation medium: fermenting auxiliary materials such as magnesium sulfate, calcium carbonate, sodium chloride, glucose, soybean protein powder, monopotassium phosphate, defoaming agent and the like according to the proportion of 1: 1: 3: 3: 2: 2: 1 is added into a fermentation tank; and (3) sterilization treatment: uniformly stirring the raw materials and the fermentation auxiliary materials in the fermentation tank, heating to 121 ℃ for sterilization, wherein the sterilization time is 30 minutes, and then cooling to 35 ℃; inoculating strains: adding lactobacillus and photosynthetic bacteria into the sterilized fermentation tank, and controlling the temperature between 28 ℃ and 30 ℃; and (3) fermentation: starting stirring for 2 minutes every 3 hours in the fermentation process, starting fermentation for 9 hours, adding 10kg of 1% nuciferine in each stirring time, adding 3 times, fermenting for 30 hours, sampling and detecting nuciferine, qualitatively detecting, and stopping fermentation when no nuciferine is detected in the fermentation solution; preparing a finished product; removing solid matters and thalli from the fermentation liquor, adding an enzyme protective agent into the supernatant, performing low-temperature vacuum concentration to obtain a liquid lotus leaf carbohydrase liquid preparation, and performing low-temperature spray drying to obtain the solid fertilizer synergist.
The using method comprises the following steps:
the product is applied to oranges, is used for being mixed with zinc boron fertilizer, and improves flowering and fruit setting. The test data are as follows:
crop: and (4) citrus: the use period is as follows: buds begin to appear.
By using the product of the invention, the contents of zinc and boron in the citrus leaves and flowers are obviously improved compared with the content of the zinc and boron in the control group. The invention obviously improves the content of trace element zinc boron in plants, has obvious synergistic effect and has obvious effect on the aspects of flowering, fruit setting and the like of the plants
Example two:
preparation of a lotus leaf carbon enzyme material: the preparation method is the same as that of example 1.
The product is applied to cucumbers and is used together with macroelement water soluble fertilizer 17-17-17+ TE. The test data are as follows:
crop: cucumber, age: the melon vine is about 30cm, and the first female flower appears.
The product of the invention is mainly used for the cucumber, the plant height, the leaves and the yield of the cucumber are obviously improved compared with the control, meanwhile, the fertilizer consumption is reduced by 25 percent, and the synergistic effect is obvious.
Example three:
preparing lotus leaf carbohydrase: the preparation method is the same as that of example 1.
The product is applied to cowpea, uses humic acid macroelement for matching, and is used for promoting the growth of cowpea and improving the yield. The test data are as follows:
crop: cowpea, age: starting (about 40cm) of pole climbing
The cowpea has dark green leaves, thick and strong stems, long and large cowpea strips, good selling effect, high yield and remarkable economic and social benefits.
Example 4:
preparing lotus leaf carbohydrase: the preparation method is the same as that of example 1.
The product is applied to corn and is matched with a compound fertilizer to be used as a base fertilizer. The test data are as follows:
crop: corn, age: base fertilizer for plowing and ditching
The product of the invention is mainly embodied in the performance of corn, the emergence of the corn seedlings are regular, the leaf color is dark green, the stems are thick and strong, the lodging resistance is realized, the corn seeds are full, the weight of the corn seeds is heavier, the corn seeds are reduced by 20 percent compared with the control group fertilizer, and the economic benefit and the social benefit are very obvious
And (4) judging the standard: compared with the conventional crop using only the fertilizer, the growth of the crop using the lotus leaf carbohydrase fertilizer synergist not only increases the fruiting rate, but also has higher product quality, stronger impression and better economic efficiency and social benefit.
The invention has the beneficial effects that: the preparation method and the application method of the fertilizer synergist increase the extraction efficiency and expand the molecular spectrum of effective components by utilizing a microbial fermentation and transformation process, have milder action effect, and simultaneously increase the functions of other aspects of the product by beneficial components such as microbial metabolites, mycoprotein and the like, thereby not only improving the synergism, but also greatly enhancing the functional effect of the product. Most importantly, the cost of synergistic raw materials is greatly reduced while the effect is ensured, the safety and the non-toxicity of the product are higher in standard vertebra by adopting a microbial fermentation conversion process, the using method is simpler, the product can be used only by dilution, and the purpose of convenient use is achieved
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. A preparation method and an application method of a fertilizer synergist are characterized in that: the preparation method comprises the following steps:
1) preparing raw materials: adding 600kg of water into a 1000L fermentation tank, and then adding 50kg of lotus leaf dry powder;
2) preparing a fermentation medium: fermenting auxiliary materials such as magnesium sulfate, calcium carbonate, sodium chloride, glucose, soybean protein powder, monopotassium phosphate, defoaming agent and the like according to the proportion of 1: 1: 3: 3: 2: 2: 1 is added into a fermentation tank;
3) and (3) sterilization treatment: uniformly stirring the raw materials and the fermentation auxiliary materials in the fermentation tank, heating to 121 ℃ for sterilization, wherein the sterilization time is 30 minutes, and then cooling to 35 ℃;
4) inoculating strains: adding lactobacillus and photosynthetic bacteria into the sterilized fermentation tank, and controlling the temperature between 28 ℃ and 30 ℃;
5) and (3) fermentation: starting stirring for 2 minutes every 3 hours in the fermentation process, starting fermentation for 9 hours, adding 10kg of 1% nuciferine in each stirring time, adding 3 times, fermenting for 30 hours, sampling and detecting nuciferine, qualitatively detecting, and stopping fermentation when no nuciferine is detected in the fermentation solution;
6) preparing a finished product; removing solid matters and thalli from the fermentation liquor, adding an enzyme protective agent into the supernatant, performing low-temperature vacuum concentration to obtain a liquid lotus leaf carbohydrase liquid preparation, and performing low-temperature spray drying to obtain the solid fertilizer synergist.
2. A preparation method and an application method of a fertilizer synergist are characterized in that: the application method comprises the following steps:
1) preparing a liquid medicine: mixing fertilizers and fertilizer synergists required by different crops, and then diluting to prepare a usable liquid medicine;
2) the first use: adding the prepared liquid medicine into a medicine spraying instrument or a medicine dripping instrument, and performing primary spraying or drip irrigation on crops according to the growth periods of different crops;
3) and (3) continuous use: the liquid medicine is used once after 10 days, and the using process is consistent with the primary using process.
3. The method for preparing and using a fertilizer synergist according to claim 1, characterized in that: the lotus leaf dry powder is dry leaf of Nymphaeaceae plant Nelumbo nucifera Gaertn, collected in sunny days in early autumn, the temperature of collection is controlled at 25-28 deg.C, when dried to be seventy-eight dry, petioles are removed, and the lotus leaf dry powder is folded into a semi-circle or fan shape, dried and crushed.
4. The method for preparing and using a fertilizer synergist according to claim 1, characterized in that: the enzyme keep-alive agent is a combination of maltitol and sorbitol, and the ratio of the maltitol to the sorbitol is 1: 1.5.
5. the method for preparing and using a fertilizer synergist according to claim 1, characterized in that: the nuciferine comprises alkaloid extracted from folium Nelumbinis and its derivatives, including flavone and polysaccharide.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113603523A (en) * | 2021-07-05 | 2021-11-05 | 河南正达作物保护有限公司 | Preparation and application method of vigorous-growth-controlling fertilizer |
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