CN113755330A

CN113755330A - Tumor tissue cell bionic culture system and method

Info

Publication number: CN113755330A
Application number: CN202111169493.8A
Authority: CN
Inventors: 马一杰
Original assignee: Henan Cancer Hospital
Current assignee: Henan Cancer Hospital
Priority date: 2021-10-08
Filing date: 2021-10-08
Publication date: 2021-12-07

Abstract

The invention belongs to the technical field of cell biology or tissue engineering, and discloses a bionic tumor tissue cell culture system which comprises a material conveying module, wherein the material conveying module is communicated with an incubator through a liquid conveying pipe, the incubator is communicated with a gas conveying system and a waste liquid tank through a gas conveying pipe and a liquid conveying pipe, the material conveying module comprises a culture liquid box, and the culture liquid box is communicated with an oxygenator and a peristaltic pump in sequence through the liquid conveying pipe and then is communicated with the incubator; the gas transmission system comprises a buffer tank, wherein the gas inlet end of the buffer tank is communicated with a nitrogen bottle and a carbon dioxide bottle through a gas transmission pipe.

Description

Tumor tissue cell bionic culture system and method

Technical Field

The invention belongs to the technical field of cell biology or tissue engineering, and particularly relates to a bionic culture system and method for tumor tissue cells.

Background

Tissue cell culture is an important method for studying normal, diseased and malignant cells; the whole process of activation, proliferation, differentiation, migration, maturation, senescence, abscission and even cell death of different types of cells and the regulation mechanism thereof can be observed, set or changed under specific conditions in vitro by using tissue and cell culture technology; tissue cell culture has played an important driving role in the development of basic medical science research and improvement of clinical treatment in the past 50 years; studies have shown that different types of tumors have different biological properties; tumors of the same type, with different individuals having different biological characteristics; in order to research the biological characteristics of tumor tissue cells of each individual, such as the sensitivity of the tumor tissue cells to radiotherapy and chemotherapy, and provide a basis for diagnosis and treatment of tumor patients, the culture of primary tumor biopsy cells is necessary; the in-vivo microenvironment is simulated, firstly, the tumor cells leaving the human body are usually subjected to anoikis due to the change of the nutrient suction mode and the survival microenvironment, so that the in-vivo microenvironment is firstly considered to be simulated during the in-vitro tumor tissue cell culture, which is the key for the survival of the in-vitro cultured tumor tissue cells.

The conventional tissue cell culture method is to culture cells in a planar, i.e., two-dimensional, space state, which is a simple and easy method to observe the cultured cells to be studied and to obtain a certain degree of cell proliferation. However, the cells are easily crowded when growing in this state, which results in the inhibition of contact between cells and the reduction of the surface area of cells exposed to the culture solution, and the cell adhesion area is limited; due to the lack of the necessary conditions specific to the growth of cells and tissues in vivo, the interaction between cells and between cells, and between cells and cell matrices, the proliferation of cells is limited, the function of cells is low, and the formation of tissue frameworks and the sufficient expression of cell functions similar to those in the living state cannot be achieved; although some improvements, such as culturing cells on natural materials related to basement membrane components, cannot achieve long-term growth and terminal differentiation of cells and sufficient expression of cell functions, a two-dimensional monolayer cell culture cannot produce three-dimensional tissues and organs, but only cell expansion, and cells often become under-differentiated cells and lack the functions of normal cells.

The three-dimensional tissue cell culture is to culture the cells on a three-dimensional cell culture material or a scaffold prepared in advance or in a biodegradable three-dimensional space structure, and then culture the cells; the structure and the environment of the three-dimensional space can provide larger surface area for cell adhesion, and cell contact inhibition during monolayer two-dimensional space culture can be reduced or avoided; meanwhile, the three-dimensional space provides a growth environment similar to that in a body for the growth of cells, fully ensures the interaction between the cells and an extracellular matrix, is beneficial to the migration and movement of the cells, and maintains the phenotype of differentiated cells; in conclusion, the three-dimensional structure and environment of tissue cell culture promote cell adhesion and proliferation, morphological maturation, and cell function improvement.

Although the culture systems of various different three-dimensional spaces adopted at present are obviously improved compared with a two-dimensional space culture method, the culture systems still have some defects; the technology of using collagen as a scaffold has a great limitation, that is, because cells in the deep part of the scaffold lack nutrition, the cells tend to grow on the surface of the collagen scaffold, so that a more perfect three-dimensional tissue cell space culture system is urgently needed to be established, the system is beneficial to the proliferation and maturation of the cells, the observation of the cells and the separation of the cells from the culture system, the microbial pollution is avoided in the culture process, the automation degree of the traditional three-dimensional culture system is low, and the culture environment cannot be observed and adjusted in real time.

Disclosure of Invention

The invention aims to provide a tumor tissue cell bionic culture system and a tumor tissue cell bionic culture method, wherein an oxygenator is additionally arranged in a material conveying module to simulate a gas conveying mode that oxygen carried by human blood enters tissues, a gas conveying system is used for providing an environment of internal pressure of the human body and a simulated gas environment, disinfection devices are arranged in the material conveying module and the gas conveying system to avoid microbial pollution, and a three-dimensional support is used as a tissue environment with vivid cells, so that the research on the growth of tumor cells in the tissues is facilitated.

Based on the purpose, the invention adopts the following technical scheme: a bionic tumor tissue cell culture system comprises a material conveying module, wherein the material conveying module is communicated with an incubator through a liquid conveying pipe, the incubator is communicated with a gas conveying system and a waste liquid tank through a gas conveying pipe and a liquid conveying pipe, the material conveying module comprises a culture liquid box, and the culture liquid box is communicated with the incubator after being sequentially communicated with an oxygenator and a peristaltic pump through the liquid conveying pipe; the gas transmission system comprises a buffer tank, the gas inlet end of the buffer tank is communicated with a nitrogen bottle and a carbon dioxide bottle through a gas transmission pipe, and the gas outlet end of the buffer tank is communicated with the liquid transmission pipe through the gas transmission pipe; a recovery pump is arranged on the liquid conveying pipe between the waste liquid tank and the incubator.

Preferably, the incubator comprises an incubator shell, a heat insulation structure connected with the incubator shell is arranged in the incubator, the heat insulation structure is cylindrical, a controller is arranged in a region between an included angle of the outer side surface of the heat insulation structure and the incubator shell, a motor is arranged in a region between the outer side surface of the heat insulation structure and the incubator shell and corresponding to the controller, and the motor is matched with the heat insulation structure; the heat preservation structure is internally provided with a culture chamber, and the heat preservation structure is in sliding fit with the culture chamber.

Preferably, the lower end of the heat insulation structure is provided with a heat insulation box bracket fixedly connected with the incubator shell, the heat insulation box bracket is provided with a transmission belt through hole matched with the motor, and the middle part of the lower end of the heat insulation structure is provided with a rotating shaft connected with the motor through the transmission belt; a heating mechanism is arranged on the lower end face in the heat preservation structure, a culture room net rack connected with the heat preservation structure is arranged above the heating mechanism, and the culture room net rack is of a hollow structure; a culture chamber snap ring which is in sliding fit with the culture chamber is arranged on the inner side surface above the culture chamber net rack of the heat preservation structure, a sealing ring which is tightly attached to the culture chamber is arranged at the upper end of the heat preservation structure, the width of the sealing ring is the same as that of the culture chamber snap ring, a water inlet and a water outlet are arranged on the sealing ring, and an air layer is arranged in the sealing ring; a micro water pump communicated with the water outlet through a liquid conveying pipe is also arranged in the heat preservation structure; an air layer communicated with the sealing ring is arranged in the side wall of the heat preservation mechanism.

Preferably, heating mechanism includes the screw support that is connected with heat preservation mechanism lower terminal surface, screw support middle part is equipped with the propeller structure who is connected with the axis of rotation, four edges of screw support up end are equipped with the connecting piece, the connecting piece upper end is equipped with the hot plate that is hollow out construction, be equipped with on the hot plate and be platelike fin, the hot plate is mutually perpendicular with the fin, hot plate one end is equipped with the heating frame that parallels with the heating panel, the heating frame closely laminates and is connected with the hot plate with the cooling panel in the outside, the hot plate upper end is equipped with and is used for having insulating buckler.

Preferably, the culture chamber comprises a shell, a culture medium and a culture medium sealing shell are arranged in the shell, a handle is arranged on the upper end face of the culture medium sealing shell, a culture chamber nutrient solution disinfection mechanism, a waste solution port and a cell injection pipeline which are communicated with the culture medium are arranged on the culture medium sealing shell, the culture chamber nutrient solution disinfection mechanism comprises an ultraviolet lamp which is arranged along the periphery of the outer side face of the infusion tube, and the upper end and the lower end of the ultraviolet lamp are provided with protective covers which are in interference fit with the infusion tube; a sealing nut matched with the culture medium is arranged on the culture medium sealing shell; the culture medium comprises a waste liquid layer, a culture medium layer, a sensor layer and a protective shell layer from bottom to top in sequence, the diameter of the waste liquid layer is smaller than that of the culture medium layer, the waste liquid layer is provided with an inwards concave waste liquid cavity, the outer side surface of the waste liquid cavity is provided with a liquid conveying pipe, the liquid conveying pipe is arranged in the side wall of the shell and is communicated with the recovery pump, the upper end of the cavity wall of the waste liquid cavity is connected with a culture medium layer, the upper end surface of the cavity wall of the waste liquid cavity is bonded with the lower end surface of the culture medium layer, the culture medium layer is inwards sunken to form a culture cavity, culture modules are arranged in the culture cavity, the plurality of culture modules can be combined in a net shape, the upper end surface of the culture cavity wall of the culture medium layer is bonded with the lower end surface of a sensor layer, and a culture unit temperature sensor, a culture unit pressure sensor, a culture unit oxygen content sensor and a culture unit carbon dioxide concentration sensor which are matched with the culture medium layer are arranged on the sensor layer; the sensor layer and the protective shell layer are fixed through adhesion, and the upper end of the protective shell layer is provided with a sealing bolt matched with a sealing nut.

Preferably, the culture module is annular, one side of the culture module is provided with a culture medium inlet communicated with the material conveying module through a liquid conveying pipe, one side with an included angle of 180 degrees with the culture medium inlet is provided with a waste liquid outlet communicated with the waste liquid cavity, one side with an included angle of 90 degrees with the culture medium inlet is provided with a cell inlet, the culture module comprises an annular enclosure which is communicated with the culture medium inlet, the waste liquid outlet and the cell inlet through a connecting channel, the outlet of the culture medium inlet is provided with a nutrient solution main channel, the nutrient solution main channel is expanded into two primary sub-channels and then communicated with the enclosure, an osmotic membrane is arranged in the enclosure, the primary sub-channels are expanded into a plurality of mutually communicated secondary sub-channels between the enclosure and the osmotic membrane after being communicated with the enclosure, an octagonal frame is arranged in the osmotic membrane, at least one microcolumn is arranged along the edge of the frame in the osmotic membrane, and a microcolumn channel is arranged between the microcolumns, gaps among the micro-column frames are three-stage sub-channels; a cell main channel is arranged at the outlet of the cell inlet, the cell main channel is unfolded into two primary sub-channels and then communicated with the enclosure, the primary sub-channels are unfolded between the enclosure and the permeable membrane into non-communicated secondary sub-channels after being communicated with the enclosure, and the secondary sub-channels are communicated with the permeable membrane through the cells; the permeable membrane is internally provided with a culture unit, the culture unit comprises at least one hollow three-dimensional support, the three-dimensional support is cylindrical, a plurality of cylindrical three-dimensional supports with different diameters are tightly attached, and the three-dimensional support is provided with through holes which are annularly arranged along the axis.

Preferably, the material conveying module comprises a culture solution box, the culture solution box is communicated with an air pump through an air pipe, an air disinfection mechanism is arranged on the air pipe between the culture solution box and the air pump, a liquid inlet is arranged at the upper end of the culture solution box, a pressure gauge is further arranged at the upper end of the culture solution box, the pressure gauge is connected with a controller, the culture solution box is barrel-shaped, at least one ultraviolet lamp tube is arranged in the barrel wall, and a weighing mechanism is arranged at the bottom of the culture solution box; the outlet of the culture solution box is communicated with an oxygenator through a liquid conveying pipe, the oxygenator is communicated with a peristaltic pump through a liquid conveying pipe, and the peristaltic pump is communicated with the culture chamber through a liquid conveying pipe.

Preferably, the gas transmission system comprises a gas source, the gas source comprises a carbon dioxide bottle and a nitrogen bottle, the carbon dioxide bottle and the nitrogen bottle have the same structure, the nitrogen bottle is spirally provided with a detection line along the inner part of the bottle body, the detection line is connected with a monitoring mechanism arranged at the mouth of the nitrogen bottle, and the mouth of the nitrogen bottle is provided with a control valve; still include the buffer tank that is connected through the gas-supply pipe with the air supply, be equipped with flow meter 24 on the gas-supply pipe between air supply and the buffer tank, be equipped with the sensor module that is connected with the controller on the buffer tank, the sensor module includes carbon dioxide concentration sensor, nitrogen gas concentration sensor and buffer tank pressure sensor, the inside upper end of buffer tank is equipped with the ultraviolet lamp group, the buffer tank passes through the gas-supply pipe and peristaltic pump and cultivates the transfer line intercommunication that the room communicates, is equipped with the solenoid valve on the gas-supply pipe.

Preferably, the buffer tank is communicated with a liquid conveying pipe communicated with the culture solution box and the oxygenator through a gas conveying pipe, the gas conveying pipe is provided with an electromagnetic valve,

a method for using a bionic culture system of tumor tissue cells comprises the following steps:

(1) taking the culture chamber out of the incubator through a handle, putting all parts of the culture chamber into a disinfection mechanism for sterilization, wiping and disinfecting all interfaces of a material conveying module, a gas conveying system and a waste liquid tank through 75% alcohol cotton, sequentially communicating a peristaltic pump, an oxygenator, a culture solution box, an air pump and an air disinfection mechanism of the material conveying module through a liquid conveying pipe and a gas conveying pipe, communicating a carbon dioxide bottle and a buffer tank of a nitrogen bottle of the gas conveying system, and then communicating the nitrogen bottle with the culture chamber;

(2) electrifying to supply power to the incubator, the material conveying module, the gas conveying system and the recovery pump of the waste liquid tank, carrying out data self-check on the material conveying module, the gas conveying system, the heat insulation structure and the culture room through a controller of the incubator, and then sending data to the client through a communication module of the controller;

(3) modifying the operation parameters of the material conveying module, the gas conveying system, the heat insulation structure and the culture chamber through the client, and then carrying out work adjustment on the material conveying module, the gas conveying system, the heat insulation structure and the culture chamber according to the set parameters to obtain corresponding indexes;

(4) after the operation parameters of the material conveying module, the gas transmission system, the heat insulation structure and the culture chamber are adjusted in place, the working processes of the material conveying module, the gas transmission system, the heat insulation structure and the culture chamber are controlled through the client, cultured cells enter a culture medium layer through a cell inlet, and then the flow rate of nutrient solution, the flow rate of gas, the oxygen content and the carbon dioxide content are adjusted according to data fed back by the culture medium layer so as to meet the growth requirement of tumor cells;

(5) the growth of the cells was observed and recorded by microscope at intervals during the culture.

Compared with the prior art, the invention has the following beneficial effects: the invention can monitor the heat preservation data in real time through the heat preservation structure, accelerate the convection speed of water and accelerate the heating efficiency of water in the heating process of distilled water through the thrust propeller structure, the three-dimensional bracket of the invention adopts a porous fiber structure, adopts transparent plant fiber, is convenient for cell adhesion and proliferation, forms a stable and uniform flowing microenvironment in the culture module through the series-parallel coupling of the culture module, simulates various factors and combines the measured data by using simulation software to control the variable method through the factors of the shape, the size, the number of microcolumns, the size of the microcolumn channel, the shape and the size of the microcolumn channel and the like to simulate the flow rate of the culture solution in the culture module and influence on the oxygen content, determines the variable range of the inner frame of the culture module to be 6-10 and the diameter range of the frame size to be 800-, the number of the gaps is 20-40, the size of the micro-column channel is 4-8um, and the flow rate of the nutrient solution is 5000-7000 um/s; according to the invention, the ultraviolet disinfection device is arranged on the material conveying module and the gas conveying system, so that the nutrient solution and the gas can be disinfected, and the nutrient solution and the gas can be prevented from polluting a culture medium with microorganisms;

in the present invention, cells include, but are not limited to: nerve cells, muscle cells, pancreatic islet cells, liver cells, intestinal cells, kidney cells, brain cells, heart cells, muscle cells, bone cells, liver cells, stomach cells, skin cells, urogenital cells, nervous system cells, immune system cells, spleen cells, bone marrow cells, lymph node cells;

the tumor cells are various benign or malignant tumor cells, such as various liver cancer cells, breast cancer cells, stomach cancer cells, prostate cancer cells, lung cancer cells, brain tumor cells, ovarian cancer cells, bone tumor cells, colon cancer cells, thyroid tumor cells, mediastinal tumor cells, small intestine tumor cells, kidney tumor cells, adrenal tumor cells, bladder tumor cells, testicular tumor cells, malignant lymphoma cells, multiple myeloma cells and nervous system tumor cells;

the tumor cell bionic culture system is used for observing the growth, proliferation, differentiation, maturation, movement or migration of various tumor cells; the infiltration and metastasis of tumors are one of the biological characteristics of malignant tumors, are the expression of a series of processes such as tumor cell adhesion, enzymatic degradation, movement, intrastromal proliferation and the like, are the results of the interaction of tumor cells and extracellular matrix under the regulation of various factors of a host, and the tumor invasion and metastasis are the most dangerous stages in the process of tumor occurrence and evolution; the invasion and the metastasis of the cancer cells are complex tumor pathogenesis problems, the invasion and the metastasis of the cancer cells are comprehensive actions and development processes of multiple stages, multiple steps, multiple factors, multiple genes and the like, and the involved aspects are wide, including the actions of normal cells initiated by carcinogenic factors and carcinogenic factors, the loss of control of growth, malignant change, invasion and metastasis of the normal cells; the metastatic focus can invade and metastasize again after being formed until the host dies;

tumor metastasis involves 3 major steps of detachment, transport and growth, however, it is a complex process with at least the following steps: under the condition that various conditions are available, the movement of tumor cells and the movement of amoeba are beneficial to the tumor cells to break through a basement membrane and enter the gaps of surrounding tissues, and after the tumor cells are separated from a primary tumor group, the tumor cells are adhered to invade the basement membrane and infiltrate and grow in surrounding interstitium; after the tumor cells enter the interstitium, various metabolic substances are continuously secreted and discharged to the interstitium by depending on information, nutrition and other supports given by the interstitium, so that the close relationship between the tumor cells and the surrounding interstitium is formed, and the tumor is continuously proliferated and developed;

malignant tumor cells can survive and grow at secondary sites, and depend on the characteristics of the tumor cells and the action of a plurality of factors in a host body, the characteristics and the factors provide proper matrix and environment for survival and growth of the cells for metastasis formation, and metastasis can be formed after the cancer cells are separated from tumor parent bodies to the formation of metastasis by various complex processes and mechanisms such as cell-cell interaction, cell-matrix interaction, escape of an organism immune mechanism, adaptation to a new environment and the like, so that finally only a few tumor cells with high metastasis capacity form the metastasis;

at present, the adhesion, the motility and the secretion of degrading enzymes of tumor cells are the most studied, and the whole process of infiltration and metastasis is difficult to observe on human bodies, so when the occurrence mechanism of tumor infiltration and metastasis is researched, the bionic culture system of the tumor cells can systematically research the in-situ movement and the ectopic movement of the tumor cells in vitro, the separation of the tumor cells and the loss of cell contact inhibition, the effect of stroma on the tumor infiltration, and the effect of tumor cell products and other related components.

Drawings

FIG. 1 is a schematic diagram of a tumor tissue cell biomimetic culture system according to the present invention;

FIG. 2 is a schematic view of an incubator of the present invention;

FIG. 3 is a cross-sectional view of the insulation structure of the present invention;

FIG. 4 is a schematic view of a heating configuration of the present invention;

FIG. 5 is a schematic view of a heat sink of the present invention;

FIG. 6 is a schematic view of a rack of a culture chamber of the present invention;

FIG. 7 is a schematic view of a gas delivery system of the present invention;

FIG. 8 is a schematic view of a nitrogen cylinder of the present invention;

FIG. 9 is a schematic view of a surge tank of the present invention;

FIG. 10 is a schematic view of a culture tank of the present invention;

FIG. 11 is a schematic view of a culture chamber of the present invention;

FIG. 12 is a schematic view of a nutrient solution sterilization mechanism of the culture chamber of the present invention;

FIG. 13 is a schematic illustration of a culture medium of the present invention;

FIG. 14 is a schematic illustration of a waste layer of the present invention;

FIG. 15 is a schematic representation of a media layer of the present invention;

FIG. 16 is a schematic view of a culture module assembly according to the present invention;

FIG. 17 is a schematic view of a culture module of the present invention;

FIG. 18 is a schematic view of a culture unit of the present invention;

FIG. 19 is a top view of a culture unit of the present invention;

FIG. 20 is a schematic view of a sensor layer of the present invention;

FIG. 21 is a schematic view of a tumor tissue cell biomimetic culture system according to the second embodiment of the present invention.

In the figure: incubator 1, incubator housing 2, controller 3, water inlet 4, incubator 5, handle 501, incubator nutrient solution disinfection mechanism 502, protective cover 503, ultraviolet lamp 504, culture medium 505, waste liquid layer 506, culture medium layer 507, sensor layer 508, protective cover layer 509, incubator sealing shell 510, waste liquid cavity 511, culture medium liquid inlet 512, culture module 513, cell inlet 514, waste liquid outlet 515, nutrient solution main channel 516, primary subchannel 517, microcolumn channel 518, microcolumn 519, cell main channel 520, culture unit 521, permeable membrane 522, three-dimensional support 523, through hole 524, culture unit temperature sensor 525, culture unit pressure sensor 526, culture unit oxygen content sensor 527, culture unit carbon dioxide concentration sensor 528, temperature sensor 6, incubator support 7, heating mechanism 8, propeller support 801, connector 802, propeller structure 803, Heating frame 804, cooling fin 805, waterproof cover 806, heating plate 807, rotating shaft 808, micro water pump 9, motor protective housing 10, motor 11, water outlet 12, culture chamber support 13, heat preservation structure 14, air layer 1401, culture chamber net rack 1402, culture chamber snap ring 1403, peristaltic pump 15, oxygenator 16, culture solution box 17, liquid inlet 1701, ultraviolet lamp 1702, pressure gauge 1703, weighing mechanism 1704, air pump 18, air disinfection mechanism 19, nitrogen gas bottle 20, probe 2001, control valve 2002, carbon dioxide bottle 21, monitoring mechanism 22, gas pipe 23, flow rate device 24, buffer tank 25, ultraviolet lamp set 2501, sensor module 26, carbon dioxide concentration sensor 2601, nitrogen concentration sensor 2602, buffer tank pressure sensor 2603, electromagnetic valve 27, waste liquid tank 28, recovery pump 29, and infusion tube 30.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to examples and accompanying drawings, and the exemplary embodiments and descriptions thereof are only used for explaining the present invention and are not meant to limit the present invention.

Example one

As shown in fig. 1-20: a bionic tumor tissue cell culture system comprises a material conveying module, wherein the material conveying module is communicated with an incubator 1 through a liquid conveying pipe 30, the incubator 1 is communicated with a gas conveying system and a waste liquid tank 28 through a gas conveying pipe 23 and the liquid conveying pipe 30, the material conveying module comprises a culture liquid box 17, and the culture liquid box 17 is communicated with the incubator 1 after being sequentially communicated with an oxygenator 16 and a peristaltic pump 15 through the liquid conveying pipe 30; the gas transmission system comprises a buffer tank 25, the gas inlet end of the buffer tank 25 is communicated with a nitrogen bottle 20 and a carbon dioxide bottle 21 through a gas transmission pipe 23, and the gas outlet end of the buffer tank 25 is communicated with a liquid transmission pipe 30 through the gas transmission pipe 23; a recovery pump 29 is provided on a liquid transfer tube 30 between the waste liquid tank 28 and the incubator 1.

The incubator 1 comprises an incubator shell 2, a heat insulation structure 14 connected with the incubator shell 2 is arranged in the incubator 1, the heat insulation structure 14 is cylindrical, a controller 3 is arranged in a region between the outer side surface of the heat insulation structure 14 and an included angle of the incubator shell 2, a motor 11 is arranged in a region between the outer side surface of the heat insulation structure 14 and the included angle of the incubator shell 2 and corresponds to the controller 3, and the motor 11 is matched with the heat insulation structure 14; the heat preservation structure 14 is internally provided with a culture chamber 5, and the heat preservation structure 14 is in sliding fit with the culture chamber 5.

The lower end of the heat preservation structure 14 is provided with a heat preservation box bracket 7 fixedly connected with the incubator casing 2, the heat preservation box bracket 7 is provided with a transmission belt through hole matched with the motor 11, and the middle part of the lower end of the heat preservation structure 14 is provided with a rotating shaft 808 connected with the motor 11 through a transmission belt; a heating mechanism 8 is arranged on the lower end face in the heat insulation structure 14, a culture room rack 1402 connected with the heat insulation structure 14 is arranged above the heating mechanism 8, and the culture room rack 1402 is of a hollow structure; a culture chamber snap ring 1403 in sliding fit with the culture chamber 5 is arranged on the inner side surface above the culture chamber net rack 1402 of the heat preservation structure 14, a sealing ring tightly attached to the culture chamber 5 is arranged at the upper end of the heat preservation structure 14, the width of the sealing ring is the same as that of the culture chamber snap ring 1403, a water inlet 4 and a water outlet 12 are arranged on the sealing ring, and an air layer 1401 is arranged in the sealing ring; a micro water pump 9 communicated with the water outlet 12 through a liquid conveying pipe 30 is also arranged in the heat preservation structure 14; an air layer 1401 communicated with the sealing ring is arranged in the side wall of the heat preservation mechanism 14.

Heating mechanism 8 includes the screw support 801 that is connected with the lower terminal surface of heat preservation mechanism 14, screw support 801 middle part is equipped with the screw structure 803 that is connected with axis of rotation 808, four edges of screw support 801 up end are equipped with connecting piece 802, the connecting piece 802 upper end is equipped with the hot plate 807 that is hollow out construction, be equipped with the fin 805 that is platelike on the hot plate 807, hot plate 807 is mutually perpendicular with fin 805, hot plate 807 one end is equipped with the heating frame 804 that parallels with the heating panel 805, heating frame 804 closely laminates with the heating panel 805 in the outside and is connected with hot plate 807, the hot plate 804 upper end is equipped with and is used for having insulating buckler 806.

The culture chamber 5 comprises a shell, a culture medium 505 and a culture medium sealing shell 510 are arranged in the shell, a lifting handle 501 is arranged on the upper end face of the culture medium sealing shell 510, a culture chamber nutrient solution disinfection mechanism 502 communicated with the culture medium 505, a waste liquid port and a cell injection pipeline are arranged on the culture medium sealing shell 510, the culture chamber nutrient solution disinfection mechanism 502 comprises an ultraviolet lamp 504 along the periphery of the outer side face of the infusion tube 30, and protective covers 503 in interference fit with the infusion tube 30 are arranged at the upper end and the lower end of the ultraviolet lamp 504; a sealing nut matched with the culture medium 505 is arranged on the culture medium sealing shell 510; the culture medium 505 comprises a waste liquid layer 506, a culture medium layer 507, a sensor layer 508 and a protective shell layer 509 in sequence from bottom to top, the diameter of the waste liquid layer 506 is smaller than that of the culture medium layer 507, the waste liquid layer 506 is provided with an inward concave waste liquid cavity 511, the outer side surface of the waste liquid cavity 511 is provided with a liquid conveying pipe 30, the liquid conveying pipe 30 is arranged in the side wall of the shell, the liquid conveying pipe 30 is communicated with a recovery pump 29, the upper end of the cavity wall of the waste liquid cavity 511 is connected with the culture medium layer 507, the upper end surface of the cavity wall of the waste liquid cavity 511 is adhered to the lower end surface of the culture medium layer 507, the culture medium layer 507 is inward concave to form a culture cavity, the culture modules 513 are arranged in the culture cavity, the plurality of the culture modules 513 can be combined in a net shape, the upper end surface of the culture cavity wall of the culture medium layer 507 is adhered to the lower end surface of the sensor layer 508, and culture unit temperature sensors 525, culture unit pressure sensors 526 and culture medium layer 507 are arranged on the sensor layer 508 and are matched with the culture medium layer 507, A culture unit oxygen content sensor 527 and a culture unit carbon dioxide concentration sensor 528; the sensor layer 508 and the protective shell 509 are fixed by adhesion, and the upper end of the protective shell 509 is provided with a sealing bolt matched with a sealing nut.

The culture module 513 is annular, one side of the culture module 513 is provided with a culture medium liquid inlet 512 communicated with the material conveying module through a liquid conveying pipe 30, one side with an included angle of 180 degrees with the culture medium liquid inlet 512 is provided with a waste liquid outlet 515 communicated with the waste liquid cavity 506, one side with an included angle of 90 degrees with the culture medium liquid inlet 512 is provided with a cell inlet 514, the culture module 513 comprises an annular enclosure which is communicated with the culture medium liquid inlet 512, the waste liquid outlet 515 and the cell inlet 514 through a connecting channel, a nutrient solution main channel 516 is arranged at the outlet of the culture medium liquid inlet 512, the nutrient solution main channel 516 is expanded into two primary sub-channels 517 and then communicated with the enclosure, an osmotic membrane 522 is arranged in the enclosure, the primary sub-channels 517 are expanded into a plurality of mutually communicated secondary sub-channels after being communicated with the enclosure, an octagonal frame is arranged in the osmotic membrane 522, and at least one microcolumn 519 is arranged in the osmotic membrane 522 along the edge of the frame, micro-column channels 518 are arranged between the micro-columns 519, and gaps between frames of the micro-columns 519 are three-level sub-channels; a cell main channel 520 is arranged at the outlet of the cell inlet 514, the cell main channel 520 is expanded into two primary sub-channels 517 and then communicated with the enclosure, the primary sub-channels 517 and the enclosure are communicated and then expanded into non-communicated secondary sub-channels between the enclosure and the permeable membrane 522 and are communicated with the permeable membrane 522 through the secondary sub-channels of the cells; the permeable membrane 522 is internally provided with a culture unit 521, the culture unit 521 comprises at least one hollow three-dimensional support 523, the three-dimensional support 523 is cylindrical, a plurality of cylindrical three-dimensional supports 523 with different diameters are tightly attached, and the three-dimensional support 523 is provided with through holes 524 which are annularly arranged along the axis.

The material conveying module comprises a culture solution box 17, the culture solution box 17 is communicated with an air pump 18 through an air pipe 23, an air disinfection mechanism 19 is arranged on the air pipe 23 between the culture solution box 17 and the air pump 18, a liquid inlet 1701 is arranged at the upper end of the culture solution box 17, a pressure gauge 1703 is also arranged at the upper end of the culture solution box 17, the pressure gauge 1703 is connected with a controller 3, the culture solution box 17 is barrel-shaped, at least one ultraviolet lamp tube 1703 is arranged in the barrel wall, and a weighing mechanism 1704 is arranged at the bottom of the culture solution box 17; the outlet of the culture solution box 17 is communicated with an oxygenator 16 through a liquid conveying pipe 30, the oxygenator 16 is communicated with a peristaltic pump 15 through the liquid conveying pipe 30, and the peristaltic pump 15 is communicated with the culture chamber 5 through the liquid conveying pipe 30.

The gas transmission system comprises a gas source, the gas source comprises a carbon dioxide bottle 21 and a nitrogen bottle 20, the carbon dioxide bottle 21 and the nitrogen bottle 20 have the same structure, a detection line 2001 is spirally distributed along the inner part of the bottle body of the nitrogen bottle 20, the detection line 2001 is connected with a monitoring mechanism 22 arranged at the bottle opening of the nitrogen bottle 20, and a control valve 2002 is arranged at the bottle opening of the nitrogen bottle 20; still include the buffer tank 25 that is connected through gas-supply pipe 23 with the air supply, be equipped with the flow velocity ware 24 on the gas-supply pipe 23 between air supply and the buffer tank 25, be equipped with the sensor module 26 that is connected with controller 3 on the buffer tank 25, sensor module 26 includes carbon dioxide concentration sensor 2601, nitrogen gas concentration sensor 2602 and buffer tank pressure sensor 2603, the inside upper end of buffer tank 25 is equipped with ultraviolet lamp group 2501, the buffer tank 25 communicates through the transfer line 30 that gas-supply pipe 23 and peristaltic pump 15 and culture room 5 communicate, be equipped with solenoid valve 27 on the gas-supply pipe 23.

The working principle is as follows: air in the environment is pumped by an air pump 18 and compressed, the compressed air is sterilized by an air sterilizing mechanism 19, the sterilized air enters a culture solution box 17 and is used for maintaining the small-range floating of the pressure intensity of the culture solution box 17 between set values, a liquid level sensor is installed in the culture solution box 17 and detects the height of the culture solution and sends the height of the culture solution to a controller 3, meanwhile, an ultraviolet lamp 1703 of the culture solution box 17 works to sterilize and disinfect the culture solution, when the height of the liquid level in the culture solution box 17 is lower than the set alarm value, the liquid level sensor sends a signal to the controller 3, and the controller 3 controls an alarm device to send an alarm and sends alarm information to a client through a communication module;

the culture solution in the culture solution box 17 enters an oxygenator 16 for oxygenation operation, then the culture solution is conveyed to the culture chamber 5 through a peristaltic pump 15 according to a set frequency, simultaneously a carbon dioxide bottle 21 and a nitrogen bottle 20 enter a buffer tank 25 through a gas pipe 23, the buffer tank 25 mixes and buffers carbon dioxide and nitrogen to prevent severe pressure fluctuation, simultaneously sterilization and disinfection operation is carried out on the buffer gas under the action of an ultraviolet lamp group 2501 in the buffer tank 25, and the sterilized gas enters a liquid conveying pipe 30 through the gas pipe 23 and enters the culture chamber 5;

meanwhile, distilled water enters the heat-insulating structure 14 through the water inlet, the heating mechanism 8 of the heat-insulating structure 14 works, the heating frame 804 is electrified to generate heat, the heat is increased in contact area with the distilled water through the radiating fins 805, the heat propagation speed is accelerated, meanwhile, the motor 11 works, the motor 11 drives the rotating shaft 808 to work and then drives the propeller structure 803 to rotate, the propeller structure 803 drives the distilled water to flow between the upper layer and the lower layer, the heat propagation speed is further accelerated, the heat transfer efficiency is improved, the temperature of the distilled water is rapidly increased to a set temperature value, the culture chamber 5 is placed in the distilled water to carry out water bath, and the culture chamber 5 is heated and subjected to heat-insulating operation;

the culture solution passes through the culture chamber nutrient solution disinfection mechanism 502, is irradiated by the ultraviolet lamp 504 to perform disinfection and sterilization again, then enters the culture medium liquid inlet 512 under the action of the liquid conveying pipe 30, then enters the primary sub-channel 517 through the nutrient solution main channel 516, then enters the secondary sub-channel and the tertiary sub-channel in the enclosure, enters the culture unit 521 of the culture module 513, forms a stable and uniform flowing microenvironment in the culture unit 521, then sends cells or tumor cells into the culture substrate 507 through the cell inlet 514 through the suction pipe, enters the primary sub-channel 517 through the cell main channel 520 after passing through the cell inlet 514, then enters the secondary sub-channel in the enclosure, then enters the culture unit 521 of the culture module 513 through the permeable membrane 522, then is attached to the three-dimensional support 523 of the culture unit 521, simulates the growth environment of the cells or the tumor cells in a human body, then the nutrient solution absorbed by the cells or tumor cells passes through the permeable membrane 522, then passes through the third-level sub-channel and the second-level sub-channel again, then flows into the first-level sub-channel 517, then flows into the nutrient solution main channel 516, finally flows into the waste solution outlet 515, flows into the waste solution layer 506 below the culture medium layer 507, and the collected waste solution enters the waste solution tank 28 under the action of the recovery pump 29.

Example two

A bionic tumor tissue cell culture system is shown in fig. 21, as compared with the first embodiment, a culture chamber support 13 for fixedly supporting the culture chamber net rack 1402 is arranged at the lower end of the culture chamber net rack 1402, a waterproof motor protection shell 10 is arranged at the outer end of a motor 11, a buffer tank 25 is communicated with a liquid conveying pipe 30 communicated with a culture solution box 17 and an oxygenator 16 through a gas conveying pipe 23, a solenoid valve 27 is arranged on the gas conveying pipe 23, a pressure release valve is arranged on a culture chamber sealing shell 510, a lifting handle 501 is hinged with the culture chamber sealing shell 510, a cell channel communicated with a cell inlet 514 is arranged on the culture chamber sealing shell 510, a valve is arranged on the culture chamber sealing shell 510 through the cell channel, an electron microscope module is arranged in the culture chamber sealing shell 510, and an illuminating device corresponding to the electron microscope module is arranged at the lower end of a culture medium layer 507.

After the cells or tumor cells are cultured for a certain period of time in the same manner as in the example, the growth, proliferation, differentiation, maturation, movement, or migration of the tumor cells in the culture unit is observed by the electron microscope module.

EXAMPLE III

A bionic culture system method for tumor tissue cells comprises the following steps:

(1) taking the culture chamber 5 out of the incubator 1 through a handle, putting all parts of the culture chamber 5 into a disinfection mechanism for sterilization, wiping and disinfecting all interfaces of a material conveying module, a gas conveying system and a waste liquid tank 28 through 75% alcohol cotton, sequentially communicating a peristaltic pump 15, an oxygenator 16, a culture solution box 17, an air pump 18 and an air disinfection mechanism 19 of the material conveying module through a liquid conveying pipe 23 and a gas conveying pipe 30, and communicating a nitrogen bottle 20, carbon dioxide 21 and a buffer tank 25 of the gas conveying system with the culture chamber 5;

(2) electrifying to supply power to the incubator 1, the material conveying module, the gas conveying system and the recovery pump 29 of the waste liquid tank 28, carrying out data self-checking on the material conveying module, the gas conveying system, the heat insulation structure 14 and the incubator 5 through the controller 3 of the incubator 1, and then sending data to the client through the communication module of the controller 3;

(3) the operating parameters of the material conveying module, the gas conveying system, the heat insulation structure 14 and the culture chamber 5 are modified through the client, and then the material conveying module, the gas conveying system, the heat insulation structure 14 and the culture chamber 5 work according to the set parameters to adjust corresponding indexes;

(4) after the operation parameters of the material conveying module, the gas transmission system, the heat insulation structure 14 and the culture chamber 5 are adjusted in place, the working procedures of the material conveying module, the gas transmission system, the heat insulation structure 14 and the culture chamber 5 are controlled through the client, the cultured cells enter the culture medium layer 507 through the cell inlet, and then the adjustment of the flow rate of nutrient solution, the flow rate of gas, the oxygen content and the carbon dioxide content is carried out according to the data fed back by the culture medium layer 507 so as to meet the requirement of the growth of tumor cells;

The culture chamber 5 is taken out of the incubator 1 through the handle 501, then the sealed enclosure 510 of the culture chamber and the culture medium 505 are separated through the bolts, then the sealed enclosure 510 of the culture chamber and the culture medium 505 are put into a sterilization mechanism to perform sterilization operation on the culture chamber 5 by high temperature, ultraviolet rays or high energy ions, then the water inlet 4, the water outlet 12, the peristaltic pump 15, the oxygenator 16, the culture solution tank 17, the air pump 18, the air sterilization mechanism 19, the nitrogen gas bottle 20, the carbon dioxide gas bottle 21, the waste liquid tank 28 and the inlet and outlet of the recovery pump 29 are wiped and sterilized by 75% alcohol, the water inlet 4, the water outlet 12, the peristaltic pump 15, the oxygenator 16, the culture solution tank 17, the air pump 18, the air sterilization mechanism 19, the nitrogen gas bottle 20, the carbon dioxide gas bottle 21, the waste liquid tank 28 and the recovery pump 29 are connected with each other through the infusion tube 30 and the air tube 23 after the sterilization is completed, then communicated with the culture chamber 5, after the assembly is finished;

electrifying the incubator 1, the material conveying module and the gas conveying system, connecting the peristaltic pump 15, the oxygenator 16, the culture solution box 17, the air pump 18, the air sterilizing mechanism 19 of the material conveying module and various sensors in the culture solution box 17 with the controller 3, connecting the monitoring mechanism 22 of the nitrogen bottle 20, the carbon dioxide bottle 21 and the buffer tank 25 of the gas conveying system, the nitrogen bottle 20 and the carbon dioxide bottle 21, the flow rate device 24 and the electromagnetic valve 27 on the gas conveying pipe 23, the ultraviolet lamp set 2501 and the sensor module 26 in the buffer tank 25 with the controller 3, connecting the recovery pump 29 with the controller 3, connecting the temperature sensor 6 of the incubator 1, the nutrient solution sterilizing mechanism 502 of the incubator room, the culture unit temperature sensor 525, the culture unit pressure sensor 526, the culture unit oxygen content sensor 527 and the culture unit carbon dioxide concentration sensor 528 of the sensor layer 508 with the controller 3, the controller 3 is connected with the intelligent mobile equipment and the PC equipment of the client through the communication module, collects data collected by all components of the incubator 1, the material conveying module and the gas transmission system and sensors through the client, and controls and regulates all components of the incubator 1, the material conveying module and the gas transmission system through the client;

the operation parameters of each device in the incubator 1, the material conveying module and the gas conveying system are set through the client, so that the pressure, the oxygen content, the nutrient solution flow rate are 5000 + 7000um/s and the gas speed in the culture unit 521 is maintained at 20-50ul/s, then the valve of the cell channel is opened, the cells or the tumor cells are sent into the cell inlet 514 through the suction pipe and sent into the culture unit 521 in the culture substrate layer 507, then the electronic microscope module works after the culture for a set time to photograph the three-dimensional support 523 in the culture unit 521, and then the photograph is sent to the client through the controller 3 or the tumor cells in the culture unit 521 are observed and recorded through manually adjusting the electronic microscope module.

It should be noted that, for those skilled in the art, many changes and modifications can be made without departing from the spirit and scope of the invention, and the invention is not to be considered limited to the embodiments illustrated in the above description.

Claims

1. The utility model provides a bionical culture system of tumour tissue cell, includes defeated material module, and defeated material module has the incubator through the transfer line intercommunication, and the incubator has gas transmission system and waste liquid jar, its characterized in that through gas-supply pipe and transfer line intercommunication: the material conveying module comprises a culture solution box, and the culture solution box is communicated with the oxygenator and the peristaltic pump in sequence through a liquid conveying pipe and then communicated with the incubator; the gas transmission system comprises a buffer tank, the gas inlet end of the buffer tank is communicated with a nitrogen bottle and a carbon dioxide bottle through a gas transmission pipe, and the gas outlet end of the buffer tank is communicated with the liquid transmission pipe through the gas transmission pipe; a recovery pump is arranged on the liquid conveying pipe between the waste liquid tank and the incubator.

2. The biomimetic culture system for tumor tissue cells according to claim 1, wherein: the incubator comprises an incubator shell, a heat insulation structure connected with the incubator shell is arranged in the incubator, the heat insulation structure is cylindrical, a controller is arranged in a region between the outer side surface of the heat insulation structure and an included angle of the incubator shell, a motor is arranged in a region between the outer side surface of the heat insulation structure and the included angle of the incubator shell and corresponds to the controller, and the motor is matched with the heat insulation structure; the heat preservation structure is internally provided with a culture chamber, and the heat preservation structure is in sliding fit with the culture chamber.

3. The biomimetic culture system for tumor tissue cells according to claim 2, wherein: the lower end of the heat insulation structure is provided with a heat insulation box bracket fixedly connected with the incubator shell, the heat insulation box bracket is provided with a transmission belt through hole matched with the motor, and the middle part of the lower end of the heat insulation structure is provided with a rotating shaft connected with the motor through a transmission belt; a heating mechanism is arranged on the lower end face in the heat preservation structure, a culture room net rack connected with the heat preservation structure is arranged above the heating mechanism, and the culture room net rack is of a hollow structure; a culture chamber snap ring which is in sliding fit with the culture chamber is arranged on the inner side surface above the culture chamber net rack of the heat preservation structure, a sealing ring which is tightly attached to the culture chamber is arranged at the upper end of the heat preservation structure, the width of the sealing ring is the same as that of the culture chamber snap ring, a water inlet and a water outlet are arranged on the sealing ring, and an air layer is arranged in the sealing ring; a micro water pump communicated with the water outlet through a liquid conveying pipe is also arranged in the heat preservation structure; an air layer communicated with the sealing ring is arranged in the side wall of the heat preservation mechanism.

4. The biomimetic culture system for tumor tissue cells according to claim 3, wherein: heating mechanism includes the screw support that is connected with heat preservation mechanism lower terminal surface, screw support middle part is equipped with the propeller structure who is connected with the axis of rotation, four edges of screw support up end are equipped with the connecting piece, the connecting piece upper end is equipped with the hot plate that is hollow out construction, be equipped with on the hot plate and be platelike fin, the hot plate is mutually perpendicular with the fin, hot plate one end is equipped with the heating frame that parallels with the heating panel, the heating frame closely laminates and is connected with the hot plate with the radiating plate in the outside, the hot plate upper end is equipped with and is used for having insulating buckler.

5. The biomimetic culture system for tumor tissue cells according to claim 4, wherein: the culture chamber comprises a shell, a culture medium and a culture medium sealing shell are arranged in the shell, a handle is arranged on the upper end face of the culture medium sealing shell, a culture chamber nutrient solution disinfection mechanism, a waste liquid port and a cell injection pipeline which are communicated with the culture medium are arranged on the culture medium sealing shell, the culture chamber nutrient solution disinfection mechanism comprises an ultraviolet lamp which is arranged along the periphery of the outer side face of a liquid conveying pipe, and protective covers which are in interference fit with the liquid conveying pipe are arranged at the upper end and the lower end of the ultraviolet lamp; a sealing nut matched with the culture medium is arranged on the culture medium sealing shell; the culture medium comprises a waste liquid layer, a culture medium layer, a sensor layer and a protective shell layer from bottom to top in sequence, the diameter of the waste liquid layer is smaller than that of the culture medium layer, the waste liquid layer is provided with an inwards concave waste liquid cavity, the outer side surface of the waste liquid cavity is provided with a liquid conveying pipe, the liquid conveying pipe is arranged in the side wall of the shell and is communicated with the recovery pump, the upper end of the cavity wall of the waste liquid cavity is connected with a culture medium layer, the upper end surface of the cavity wall of the waste liquid cavity is bonded with the lower end surface of the culture medium layer, the culture medium layer is inwards sunken to form a culture cavity, culture modules are arranged in the culture cavity, the plurality of culture modules can be combined in a net shape, the upper end surface of the culture cavity wall of the culture medium layer is bonded with the lower end surface of a sensor layer, and a culture unit temperature sensor, a culture unit pressure sensor, a culture unit oxygen content sensor and a culture unit carbon dioxide concentration sensor which are matched with the culture medium layer are arranged on the sensor layer; the sensor layer and the protective shell layer are fixed through adhesion, and the upper end of the protective shell layer is provided with a sealing bolt matched with a sealing nut.

6. The biomimetic culture system for tumor tissue cells according to claim 5, wherein: the culture module is annular, one side of the culture module is provided with a culture medium inlet communicated with the material conveying module through a liquid conveying pipe, one side with an included angle of 180 degrees with the culture medium inlet is provided with a waste liquid outlet communicated with a waste liquid cavity, one side with an included angle of 90 degrees with the culture medium inlet is provided with a cell inlet, the culture module comprises an annular enclosure, the enclosure is communicated with the culture medium inlet through a connecting channel, the waste liquid outlet and the cell inlet, the outlet of the culture medium inlet is provided with a nutrient solution main channel, the nutrient solution main channel is expanded into two primary sub-channels and then communicated with the enclosure, an osmotic membrane is arranged in the enclosure, the primary sub-channels are expanded into a plurality of mutually communicated secondary sub-channels between the enclosure and the osmotic membrane after being communicated with the enclosure, an octagonal frame is arranged in the osmotic membrane, at least one microcolumn is arranged along the edge of the frame, and microcolumn channels are arranged between the microcolumns, gaps among the micro-column frames are three-stage sub-channels; a cell main channel is arranged at the outlet of the cell inlet, the cell main channel is unfolded into two primary sub-channels and then communicated with the enclosure, the primary sub-channels are unfolded between the enclosure and the permeable membrane into non-communicated secondary sub-channels after being communicated with the enclosure, and the secondary sub-channels are communicated with the permeable membrane through the cells; the permeable membrane is internally provided with a culture unit, the culture unit comprises at least one hollow three-dimensional support, the three-dimensional support is cylindrical, a plurality of cylindrical three-dimensional supports with different diameters are tightly attached, and the three-dimensional support is provided with through holes which are annularly arranged along the axis.

7. The biomimetic culture system for tumor tissue cells according to claim 6, wherein: the culture medium box is communicated with the air pump through a gas pipe, an air disinfection mechanism is arranged on the gas pipe between the culture medium box and the air pump, a liquid inlet is formed in the upper end of the culture medium box, a pressure gauge is further arranged at the upper end of the culture medium box and connected with the controller, the culture medium box is barrel-shaped, at least one ultraviolet lamp tube is arranged in the wall of the barrel, and a weighing mechanism is arranged at the bottom of the culture medium box; the outlet of the culture solution box is communicated with an oxygenator through a liquid conveying pipe, the oxygenator is communicated with a peristaltic pump through a liquid conveying pipe, and the peristaltic pump is communicated with the culture chamber through a liquid conveying pipe.

8. The biomimetic culture system for tumor tissue cells according to claim 7, wherein: the gas transmission system comprises a gas source, the gas source comprises a carbon dioxide bottle and a nitrogen bottle, the carbon dioxide bottle and the nitrogen bottle are identical in structure, a detection line is spirally distributed in the nitrogen bottle along the inner part of the bottle body, the detection line is connected with a monitoring mechanism arranged at the opening of the nitrogen bottle, and a control valve is arranged at the opening of the nitrogen bottle; still include the buffer tank that is connected through the gas-supply pipe with the air supply, be equipped with flow meter 24 on the gas-supply pipe between air supply and the buffer tank, be equipped with the sensor module that is connected with the controller on the buffer tank, the sensor module includes carbon dioxide concentration sensor, nitrogen gas concentration sensor and buffer tank pressure sensor, the inside upper end of buffer tank is equipped with the ultraviolet lamp group, the buffer tank passes through the gas-supply pipe and peristaltic pump and cultivates the transfer line intercommunication that the room communicates, is equipped with the solenoid valve on the gas-supply pipe.

9. The biomimetic culture system for tumor tissue cells according to claim 8, wherein: the buffer tank is communicated with a liquid conveying pipe for communicating the culture solution box and the oxygenator through a gas conveying pipe, and an electromagnetic valve is arranged on the gas conveying pipe.

10. A bionic culture system method of tumor tissue cells is characterized in that: the method comprises the following steps: