CN113749613B - Probe device, optical fiber arrangement mode and optical fiber detection system - Google Patents
Probe device, optical fiber arrangement mode and optical fiber detection system Download PDFInfo
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- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0082—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes
- A61B5/0084—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence adapted for particular medical purposes for introduction into the body, e.g. by catheters
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/68—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient
- A61B5/6846—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient specially adapted to be brought in contact with an internal body part, i.e. invasive
- A61B5/6847—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient specially adapted to be brought in contact with an internal body part, i.e. invasive mounted on an invasive device
- A61B5/6848—Needles
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- A—HUMAN NECESSITIES
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- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/68—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient
- A61B5/6846—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient specially adapted to be brought in contact with an internal body part, i.e. invasive
- A61B5/6867—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient specially adapted to be brought in contact with an internal body part, i.e. invasive specially adapted to be attached or implanted in a specific body part
- A61B5/6868—Brain
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Abstract
The invention belongs to the field of optogenetic and optical fiber fluorescence recording, and particularly relates to a probe device, an optical fiber arrangement mode and an optical fiber detection system, aiming at solving the problem that the traditional optical fiber probe does not have the axial resolution capability of an optical fiber. The probe device comprises a body and an optical fiber; one end of the body is of a pointed structure, through holes for penetrating optical fibers are formed in the body, and the through holes are arranged in parallel along the length direction of the body; the cross section of the optical fiber at the tip structure leakage part is consistent with the cross section structure of the through hole at the tip structure; the body comprises a first part and a second part, one end of the first part is used for being connected with the set installation part, and the other end of the first part is connected with the second part; the second component comprises one or more end portions, one or more sealing portions; the cross-sectional areas of the adjacent end portions and the sealing portion decrease in sequence in the implantation length direction of the body. Through the setting of the multi-level working face in the direction of implanting the probe, realize that the fiber probe possesses the ability of optic fibre axial resolution.
Description
Technical Field
The invention belongs to the field of optogenetic and optical fiber fluorescence recording, and particularly relates to a probe device, an optical fiber arrangement mode and an optical fiber detection system.
Background
The implanted optical fiber guides light into and out of brain tissue to realize light regulation and light detection, and the method is an invasive research method and has the advantage of cell type specificity. The method can be divided into optogenetic, optical fiber recording and in-vivo fluorescence imaging according to the application. In the prior art, one or a plurality of optical fibers are implanted at different positions to realize optical stimulation and fluorescence recording, light emission and light collection are limited at the end faces of the optical fibers, the effective distance from the end faces is generally only 100um, and the effective distance is difficult to exceed 1mm (the maximum two-photon excitation can reach about 1 mm). The optical fiber has no distributed light emitting and receiving capability along the axial direction of the optical fiber, and also has no axial spatial resolution capability; in fact, brain tissue is three-dimensional, and has great significance for stimulating and observing in the depth direction with high resolution.
Optogenetic refers to a technique for precisely controlling the activity of specific neurons by combining optical and genetic means, and exciting light is introduced into and irradiates brain tissue through an optical fiber. However, the optical fiber probe in the prior art only emits light at the end face of the optical fiber, and has no resolution capability along the depth direction of the optical fiber implantation; even if the end face of the optical fiber probe is designed to be an inclined plane, a cone or a 45-degree lateral reflection, the stimulation can be carried out on one point, and the resolving power in the axial direction of the optical fiber is not provided.
The purpose of optical fiber recording is to detect the activity change of cells in real time; fluorescence imaging techniques based on changes in calcium ion concentration are widely used to record neuronal activity; the optical fiber probe used for optical fiber recording is the same as the optogenetic probe, and can only record signals at the end of the optical fiber no matter a single-channel product or a multi-channel product, and has no resolution capability along the depth direction (namely on the optical fiber axis) of the optical fiber implantation.
In the body fluorescence imaging, brain tissue is imaged by implanting a gradient refraction lens, and although image information can be provided, only an image near the end face of the gradient refraction lens (<300um) is recorded, and the resolution is not available along the axial direction of the gradient refraction lens.
Disclosure of Invention
In order to solve the problems in the prior art, namely to solve the problem that the conventional optical fiber probe does not have the axial resolution capability of an optical fiber, the invention provides a probe device, an optical fiber arrangement mode and an optical fiber detection system.
A first aspect of the invention provides a probe apparatus comprising a body, an optical fibre; one end of the body is of a pointed structure, and one or more through holes for the optical fibers to penetrate through are formed in the body; when the number of the through holes is multiple, the through holes are arranged in parallel along the length direction of the body; the cross section of the optical fiber at the tip structure leakage part is consistent with the cross section structure of the through hole at the tip structure;
the body comprises a first part and a second part, wherein one end of the first part is used for being connected with a set installation part, and the other end of the first part is connected with one end of the second part; the second component comprises one or more ends having a first cross-sectional area, and one or more seals having a second cross-sectional area; the first cross-sectional area of the end portion is greater than the second cross-sectional area of the adjacent sealing portion in the implanted length direction of the body.
In some preferred examples, the second part comprises a first working surface and a second working surface, and the first working surface and the second working surface are respectively arranged on two sides of the second part;
the first working surface is provided with a plurality of end parts and a plurality of sealing parts, and the adjacent end parts and the sealing parts are arranged in a downward step shape;
the second working surface is a smooth sealing surface; one end, far away from the first part, of the first working face is connected with the second working face, and the connecting surfaces of the first working face, the second working face and the second part are arranged in a triangular mode.
In some preferred examples, the second part comprises a first working surface and a second working surface, and the first working surface and the second working surface are respectively arranged on two sides of the second part;
the first working surface and the second working surface are both provided with a plurality of end parts and a plurality of sealing parts, and the adjacent end parts and the sealing parts are both arranged in a stepped manner;
the first working surface, the second working surface and the second part are connected to form an isosceles triangle.
In some preferred examples, the adjacent end portions are arranged in a fold-down manner.
In some preferred examples, the adjacent end portions are arranged in a spiral descending manner; the first component is arranged in a cylindrical shape; the second part is arranged in a conical shape.
In some preferred examples, the first member is disposed in a prismatic configuration;
the second part is arranged in a pyramid shape; a plurality of pyramid sides of the second member are each provided with a plurality of the end portions and a plurality of the sealing portions; the end face of the end part and the end face of the sealing part are both inclined cutting faces.
In some preferred examples, the first member is cylindrically disposed; the second part is arranged in a conical shape; the conical side surfaces of the second component are provided with a plurality of end parts and a plurality of sealing parts; the end face of the end part and the end face of the sealing part are both inclined cutting faces.
In some preferred examples, the first component and the second component are integrally formed or fixedly connected.
The second aspect of the present invention provides an optical fiber arrangement method, which includes a plurality of optical fibers/optical fiber bundles, wherein the plurality of optical fibers/optical fiber bundles are divided into at least two groups, and the detection end portions of the optical fibers/optical fiber bundles of each group are arranged in a staggered manner, so that the optical fibers/optical fiber bundles of each group are arranged in a step shape in a first direction; or each group of optical fibers/optical fiber bundles are spirally arranged in the first direction; or the optical fibers/optical fiber bundles of each group are arranged in a folding manner in the first direction; the first direction is an axial direction of the optical fiber/fiber bundle.
The invention provides an optical fiber detection system, which comprises an imaging device, a plurality of branch image-transmitting optical fibers and an axial distributed optical fiber probe, wherein the axial distributed optical fiber probe is connected to one end of each branch image-transmitting optical fiber and is used for detecting the end face and the axial side faces with different heights of a target; the axially distributed optical fiber probe is the probe device of any one of the first aspect; or, the arrangement of the optical fibers/optical fiber bundles adopted in the axially distributed optical fiber probe is the optical fiber arrangement described in the second aspect.
The invention has the beneficial effects that:
1) through the arrangement of the probe device, the signal at the tail end of the optical fiber can be recorded, the resolution capability along the depth direction of the optical fiber implantation can be realized, the detection effect is improved in the implantable optogenetic and optical fiber fluorescence recording, and the three-dimensional detection method has great significance for the three-dimensional detection of the specific part of the brain.
2) According to the optical fiber arrangement mode provided by the second aspect of the invention, a plurality of optical fibers/optical fiber bundles forming the probe for detection are arranged according to the mode, and the action surface of the probe for detection is arranged in a step shape, a spiral shape or a turn-back shape, so that the probe formed by only arranging the optical fibers also has axial resolution capability, and the optical fiber arrangement mode is different from the optical fiber arrangement mode in the prior art in which the detection of only one end surface can be realized, and has great significance for the detection in the medical field.
3) The invention discloses an optical fiber detection system, which can realize the detection of the end surface and the different height level surfaces of the implantation direction of a target to be detected by adopting the probe device disclosed by the first aspect of the invention or adopting the optical fiber arrangement mode provided by the second aspect of the invention for a plurality of optical fibers/optical fiber bundles at the probe action end, can carry out three-dimensional detection on the target to be detected and improve the detection accuracy.
Drawings
Other features, objects and advantages of the present application will become more apparent upon reading of the following detailed description of non-limiting embodiments thereof, made with reference to the accompanying drawings.
FIG. 1 is a perspective view of a first embodiment of a probe apparatus according to the present invention;
FIG. 2 is a perspective view of a second embodiment of the probe device of the present invention;
FIG. 3 is a perspective view of a third embodiment of a probe apparatus according to the present invention;
FIG. 4 is a perspective view of a fourth embodiment of a probe device of the present invention;
FIG. 5 is a schematic perspective view of a fifth embodiment of a probe apparatus according to the present invention;
FIG. 6 is a schematic perspective view of a sixth embodiment of a probe apparatus according to the present invention;
FIG. 7 is a schematic perspective view of a first embodiment of an optical fiber arrangement according to the present invention;
FIG. 8 is a schematic perspective view of a second embodiment of the optical fiber arrangement of the present invention;
FIG. 9 is a schematic perspective view of a third embodiment of the optical fiber arrangement of the present invention;
FIG. 10 is a schematic perspective view of a fourth embodiment of the optical fiber arrangement of the present invention;
FIG. 11 is a schematic perspective view of a fifth embodiment of the optical fiber arrangement of the present invention;
FIG. 12 is a schematic perspective view of a sixth embodiment of the optical fiber arrangement of the present invention;
FIG. 13 is a schematic structural diagram of an embodiment of an optical fiber detection system according to the present invention.
Description of the reference numerals: 1. a first member; 2. a second part, 21, an end, 22, a seal; 3. a CMOS image sensor; 4. an achromatic lens; 5. an optical filter; 6. a second light path conversion mirror; 7. a second light source; 8. an objective lens; 9. an image transmission optical fiber bundle; 10. a first light path conversion mirror; 11. a DMD module; 12. a first light source; 13. a branch image transmission optical fiber; 14. a fiber optic probe.
Detailed Description
The preferred embodiments of the present invention will be described below with reference to the accompanying drawings, and it will be understood by those skilled in the art that these embodiments are merely illustrative of the technical principles of the present invention and are not intended to limit the scope of the present invention.
A first aspect of the invention provides a probe apparatus comprising a body and an optical fibre; one end of the body is of a pointed structure, a plurality of through holes for penetrating optical fibers are formed in the body, and the through holes are arranged in parallel along the length direction of the body; the cross section of the tip structure leakage part of the optical fiber is consistent with the cross section structure of the through hole at the tip structure, namely the through hole arranged in the body is consistent with the shape of the optical fiber arranged in the body, and the practical effect of the probe device is guaranteed.
Further, the body comprises a first component and a second component, one end of the first component is used for being connected with the setting installation part, and the other end of the first component is connected with one end of the second component; the second component includes one or more ends having a first cross-sectional area, and one or more seals having a second cross-sectional area; the first cross-sectional area of the end part in the implantation length direction of the body is larger than the second cross-sectional area of the adjacent sealing part, namely, the cross-sectional area of the second part in the body is gradually reduced along the implantation direction of the probe device; the arrangement of the upper end part and the sealing part of the optical fiber probe ensures that the optical fiber installed in the through hole can detect the target to be detected through the end part, so that the resolution detection of the end surface and the axial direction of the probe is realized, particularly the endoscopic detection of the brain, aiming at the three-dimensional condition of brain tissues, the optical fiber probe in the prior art only emits light at the end surface of the same level of the optical fiber, and has no resolution capability along the depth direction of the implanted optical fiber, even if the end surface of the optical fiber probe is designed into an inclined plane, a cone or 45-degree lateral reflection and the like, only one point can be stimulated, and the resolution capability is not available in the axial direction of the optical fiber.
Further, the first and second components may be integrally formed or fixedly attached.
Furthermore, the whole probe device is in a slender needle shape, meets the requirement of medical detection, is convenient for an operator to control the operator to insert/pull out a target to be detected through the slender needle shape, and reduces the damage to be detected; in practical application, a part of the probe device for realizing detection can enter a target to be detected, and does not need to enter the target completely, so that the operation is convenient; in addition, the length and the size of the probe device can be flexibly set according to actual requirements.
The second aspect of the present invention provides an optical fiber arrangement method, which includes a plurality of optical fibers/optical fiber bundles, wherein the plurality of optical fibers/optical fiber bundles are divided into at least two groups, and the detection end portions of the groups of optical fibers/optical fiber bundles are arranged in a staggered manner, so that the groups of optical fibers/optical fiber bundles are arranged in a step shape in a first direction; or each group of optical fibers/optical fiber bundles are spirally arranged in the first direction; or each group of optical fibers/optical fiber bundles are arranged in a folding manner in the first direction; the first direction is the axial direction of the optical fiber/optical fiber bundle; the probe with axial resolution detection capability can achieve the effect through the arrangement of the optical fibers/optical fiber bundles, an accommodating device is not required to be additionally arranged, and the optical fibers/optical fiber bundles are only arranged into the integral probe with detection surfaces of different levels.
The invention provides an optical fiber detection system, which comprises an imaging device, a plurality of branch image-transmitting optical fibers and an axial distributed optical fiber probe, wherein the axial distributed optical fiber probe is connected to one end of each branch image-transmitting optical fiber and is used for detecting the end face and the axial side faces with different heights of a target; wherein, the axial distributed optical fiber probe is the probe device provided by the first aspect of the invention; or, the optical fiber/optical fiber bundle arrangement mode adopted in the axial distributed optical fiber probe is the optical fiber arrangement mode provided by the second aspect of the invention, the external limitation of the probe device is not needed, and the required probe with axial resolution capability is formed only through the arrangement of a plurality of optical fibers/optical fiber bundles, so as to detect the target to be detected.
Further, in the optical fiber arrangement manner provided by the second aspect of the present invention, the detection probe composed of different arrangement manners may be used alone, or may be used in cooperation with the probe apparatus provided by the first aspect of the present invention, and when the working surface of the probe apparatus for detection is consistent with the working surface formed by the detection end portions of the plurality of optical fibers/optical fiber bundles arranged in a staggered manner in the optical fiber arrangement manner, the working surface may be directly placed into the probe apparatus through a through hole provided in the probe apparatus, so as to form an integral body for use.
The invention is further described with reference to the following detailed description of embodiments with reference to the accompanying drawings.
Referring to FIG. 1, there is shown a perspective view of a first embodiment of a probe apparatus of the present invention; the probe device comprises a body consisting of a first component 1 and a second component 2, wherein one end of the body is of a pointed structure and is used for being inserted into a brain or other parts to be detected, and the pointed structure is designed to ensure the insertion effect and reduce the damage to the parts to be detected; a plurality of through holes for penetrating optical fibers are formed in the body, and the through holes are arranged in parallel along the length direction of the body; the other end of the body is used for the penetration of the optical fiber; the cross section of the optical fiber at the tip structure leakage part is consistent with the cross section structure of the through hole at the tip structure, so that the optical fiber penetrating through the body can be used for detecting different sites on different axial hierarchical planes of the tip structure, and the optical fiber does not depart from the body.
Further, the one end of first part 1 is used for being connected with the settlement installation department, and the other end is connected with the one end of second part 2, and in this embodiment, the one end that the second part was kept away from to the first part is used for wearing to establish of optic fibre, and perhaps still can be used to wear to establish the optic fibre of body inside and be connected with external connecting optical fibre, guarantees the transmission of optical fiber detection information.
Further, the second component 2 comprises one or more end portions 21 having a first cross-sectional area, and one or more sealing portions 22 having a second cross-sectional area; in the implantation length direction of the body, the first cross-sectional area of the end part 21 is larger than the second cross-sectional area of the adjacent sealing part 22, namely, along the to-be-implanted direction of the probe device, the structure of the body is a pointed structure, so that the optical fiber penetrating through the interior of the body is ensured to have resolution capability in the axial direction of the pointed structure, and the axial multi-point detection is realized.
Further, the second component 2 comprises a first working surface and a second working surface, and in the present embodiment, the first working surface and the second working surface are respectively arranged on the upper side and the lower side of the second component; the first working surface is provided with a plurality of end parts 21 and a plurality of sealing parts 22, and the adjacent end parts and the sealing parts are arranged in a downward step shape; the second working surface is a smooth sealing surface; one end of the first working surface, which is far away from the first part, is connected with the second working surface, and the connecting surfaces of the first working surface, the second working surface and the second part are arranged in a triangular shape; further, in this embodiment, the second part of the probe device may also be arranged in a wedge shape.
Referring to FIG. 2, there is shown a perspective view of a second embodiment of the probe apparatus of the present invention; the second component 2 comprises a first working surface and a second working surface, the first working surface and the second working surface are respectively arranged at the upper side and the lower side of the second component, a plurality of end parts 21 and a plurality of sealing parts 22 are respectively arranged on the first working surface and the second working surface, and the adjacent end parts and the sealing parts are respectively arranged in a lower step shape; the connection surfaces of the first working surface, the second working surface and the second component are arranged in an isosceles triangle.
Referring to FIG. 3, a perspective view of a third embodiment of the probe apparatus of the present invention is shown; the probe device comprises a body consisting of a first component 1 and a second component 2, wherein one end of the body is of a pointed structure and is used for being inserted into a brain or other parts to be detected, and the pointed structure is designed to ensure the insertion effect and reduce the damage to the parts to be detected; a plurality of through holes for penetrating optical fibers are formed in the body, and the through holes are arranged in parallel along the length direction of the body; the other end of the body is used for the penetration of the optical fiber; the cross section of the optical fiber at the tip structure leakage part is consistent with the cross section structure of the through hole at the tip structure, so that the optical fiber penetrating through the body can be used for detecting different sites on different axial hierarchical planes of the tip structure, and the optical fiber does not depart from the body. One end of the first component 1 is used for connecting with the set installation part, and the other end is connected with one end of the second component 2, in the embodiment, one end of the first component far away from the second component is used for the penetration of optical fibers, or can also be used for the connection of the optical fibers penetrating inside the body and external connecting optical fibers, so that the transmission of optical fiber detection information is ensured; the first component is arranged in a prism shape, and the second component is arranged in a wedge shape; the second member 2 includes a plurality of end portions 21 having a first cross-sectional area, and a plurality of seal portions 22 having a second cross-sectional area; in the implantation length direction of the body, the first cross-sectional area of the end part 21 is larger than the second cross-sectional area of the adjacent sealing part 22, namely, along the to-be-implanted direction of the probe device, the structure of the body is a pointed structure, so that the optical fiber penetrating through the interior of the body is ensured to have resolution capability in the axial direction of the pointed structure, and the axial multi-point detection is realized.
Further, the second part 2 includes first working face and second working face, and first working face and second working face set up respectively in the upper and lower both sides of second part, and all are provided with a plurality of tip 21 and a plurality of sealing 22 on first working face and the second working face, and adjacent tip 21 all is down the ladder-shaped setting with sealing 22 and be the descending formula setting of turning back between the adjacent tip 21.
Referring to FIG. 4, there is shown a schematic perspective view of a fourth embodiment of the probe apparatus of the present invention; the probe device comprises a body consisting of a first component 1 and a second component 2, wherein one end of the body is of a pointed structure and is used for being inserted into a brain or other parts to be detected, and the pointed structure is designed to ensure the insertion effect and reduce the damage to the parts to be detected; a plurality of through holes for penetrating optical fibers are formed in the body, and the through holes are arranged in parallel along the length direction of the body; the other end of the body is used for the penetration of the optical fiber; the cross section of the optical fiber at the tip structure leakage part is consistent with the cross section structure of the through hole at the tip structure, so that the optical fiber penetrating through the body can be used for detecting different sites on different axial hierarchical planes of the tip structure, and the optical fiber does not depart from the body. One end of the first component 1 is used for connecting with the set installation part, and the other end is connected with one end of the second component 2, in the embodiment, one end of the first component far away from the second component is used for the penetration of optical fibers, or can also be used for the connection of the optical fibers penetrating inside the body and external connecting optical fibers, so that the transmission of optical fiber detection information is ensured; the first part is arranged in a cylindrical shape, and the second part is arranged in a conical shape; the second member 2 includes a plurality of end portions 21 having a first cross-sectional area, and a plurality of seal portions 22 having a second cross-sectional area; in the implantation length direction of the body, the first cross-sectional area of the end part 21 is larger than the second cross-sectional area of the adjacent sealing part 22, namely, along the to-be-implanted direction of the probe device, the structure of the body is a pointed structure, so that the optical fiber penetrating through the interior of the body is ensured to have resolution capability in the axial direction of the pointed structure, and the axial multi-point detection is realized; the adjacent end parts are arranged in a spiral descending mode, the axial position of each micro-fiber in the spiral descending mode is different, and the axial resolution is higher.
Referring to fig. 5, there is shown a schematic perspective view of five embodiments of the probe apparatus of the present invention; the probe device comprises a body consisting of a first component 1 and a second component 2, wherein one end of the body is of a pointed structure and is used for being inserted into a brain or other parts to be detected, and the pointed structure is designed to ensure the insertion effect and reduce the damage to the parts to be detected; a plurality of through holes for penetrating optical fibers are formed in the body, and the through holes are arranged in parallel along the length direction of the body; the other end of the body is used for the penetration of the optical fiber; the cross section of the optical fiber at the tip structure leakage part is consistent with the cross section structure of the through hole at the tip structure, so that the optical fiber penetrating through the body can be used for detecting different sites on different axial hierarchical planes of the tip structure, and the optical fiber does not depart from the body. In the embodiment, one end of the first component 1, which is far away from the second component, is used for the penetration of the optical fiber, or can also be used for the connection between the optical fiber penetrating the body and an external connecting optical fiber, so as to ensure the transmission of optical fiber detection information; the first part is arranged in a prism shape, and the second part is arranged in a pyramid shape; the second member 2 includes a plurality of end portions 21 having a first cross-sectional area, and a plurality of seal portions 22 having a second cross-sectional area; along the direction of the probe device to be implanted, the structure of the body is a pointed structure, so that the optical fiber penetrating through the interior of the body is ensured to have resolution capability in the axial direction of the pointed structure, and the detection of multiple axial sites is realized; the conical side surfaces of the second components are provided with a plurality of end parts and a plurality of sealing parts; the end faces of the end part and the sealing part are oblique planes, so that a plurality of optical fibers of each layer are ensured to be positioned at the same axial position, and more signals can be obtained at the same axial position.
Furthermore, the probe device can be arranged in a conical step shape, the first part is arranged in a cylindrical shape, and the second part is arranged in a conical shape; the end part and the sealing part arranged on the second component are arranged in a step type at equal intervals, and the optical fibers of each layer on the step are positioned at the same axial position, so that more signals can be obtained at the same axial position.
Further, the body is in the shape of an elongated needle as a whole, so that the insertion of the target to be detected is facilitated, and in the invention, the probe device is used for being inserted into the target to be detected, so that the second part for detection in the probe device is inserted, wherein the first part can not enter the target to be detected.
Furthermore, the optical fiber is a microwire optical fiber, and a common flat-cut optical fiber, an inclined plane optical fiber, a reflection type lateral optical fiber and the like can be selected according to requirements. When the single-filament ordinary flat-cut optical fiber is adopted, in order to improve the lateral light-receiving and light-emitting capability, a reflective layer can be coated on the surface of the single-filament optical fiber and is used for reflecting lateral light into the optical fiber. After the optical fiber bundle is manufactured, the tail end of the optical fiber bundle is immersed in UV optical cement, the optical fiber bundle is taken out and then is irradiated by ultraviolet light for curing, and the cement naturally forms a micro lens on the end face, so that the lateral light collecting and emitting capacity is improved, meanwhile, the end part of the optical fiber bundle is smoother, and the damage to brain tissues is less when the optical fiber bundle is implanted.
It should be noted that, in several embodiments provided by the present invention, the adjacent end portions or sealing portions may be disposed at equal intervals or may be disposed randomly; the overall shape of the probe device can adopt wedge-like shapes, cone-like shapes and pyramid-like shapes with different shapes, and is not limited to the specific embodiments provided by the invention, and particularly, the probe device can be flexibly arranged according to practical application, and the embodiments do not limit the protection scope of the invention, as known by the technical personnel in the field.
The second aspect of the present invention provides an optical fiber arrangement method, which includes a plurality of optical fibers/optical fiber bundles, wherein the plurality of optical fibers/optical fiber bundles are divided into at least two groups, and the detection end portions of the groups of optical fibers/optical fiber bundles are arranged in a staggered manner, so that the groups of optical fibers/optical fiber bundles are arranged in a step shape in a first direction; or each group of optical fibers/optical fiber bundles are spirally arranged in the first direction; or each group of optical fibers/optical fiber bundles are arranged in a folding manner in the first direction; the first direction is the axial direction of the optical fibers/optical fiber bundles, namely the probe formed by the arrangement of the optical fibers/optical fiber bundles has axial resolution detection capability, an additional accommodating device is not needed, and the optical fiber probe can be realized by arranging the optical fibers/optical fiber bundles into an integral probe with detection surfaces of different levels only.
Referring to fig. 7 to 12, there are shown fiber optic probes 14 with axial resolution formed by different fiber arrangements. Fig. 7 shows that each group of optical fibers/optical fiber bundles is arranged in a step shape along the axial direction, and further, along the direction to be implanted, the detection end surfaces of the groups of optical fibers/optical fiber bundles form a probe with a single-surface lower step-shaped pointed structure and have the resolution detection capability of different axial depths; fig. 8 shows that along the implantation direction, the detection end surfaces of multiple groups of optical fibers/optical fiber bundles form a probe with a double-sided lower stepped pointed structure, so as to realize the resolution detection capability of different depths in the double-sided axial direction; fig. 9 shows that each group of optical fibers/optical fiber bundles is arranged in a back-turning manner in the axial direction, and further, in the direction to be implanted, the detection end surfaces of the plurality of groups of optical fibers/optical fiber bundles form a probe with a double-sided down-turning type tip structure, which has the capability of distinguishing and detecting at different axial depths, and as understood by those skilled in the art, the probe can also be arranged in a single-sided back-turning manner, and can be flexibly arranged according to the actual application requirements; fig. 10 shows that each group of optical fibers/optical fiber bundles is arranged in a spiral manner in the axial direction, and further, in the direction to be implanted, the detection end surfaces of the plurality of groups of optical fibers/optical fiber bundles form a downward spiral type pointed-end probe with resolution detection capabilities at different axial depths; fig. 11 shows that along the direction to be implanted, the detection end surfaces of a plurality of groups of optical fibers/optical fiber bundles form a probe with a three-sided downward stepped pointed structure, that is, the probe is integrally arranged in a triangular pyramid shape and has the capability of distinguishing and detecting at different axial depths, and in addition, the probe can be flexibly arranged in a single-sided shape, a double-sided shape and other polygonal pyramid shapes according to actual requirements, and details are not repeated herein; fig. 12 shows that along its to-be-implanted direction, the detection end faces of multiple groups of optical fibers/optical fiber bundles form a probe with a double-faced lower stepped tip structure, that is, the probe is integrally arranged in a conical shape and has the resolution detection capability of different axial depths.
It should be noted that, the solutions listed in the second aspect of the present invention do not limit the protection scope of the present invention, and the probe structure can be formed in other arrangements such as a cylindrical lower step shape, a wedge shape, and the like, and satisfies the requirement of having the axial resolution capability, so that details are not repeated herein.
Furthermore, the whole of the shape of the optical fiber probe formed by different arrangement modes is in a slender needle shape, so that the insertion of the target to be detected is facilitated. Further, the optical fiber bundle arrangement in the present invention may also be integrally designed in a tapered shape to form the detection probe, and the arranged optical fiber bundles are preferably arranged in parallel, as known to those skilled in the art, the optical fibers may also be directly designed in a tapered shape.
The invention provides an optical fiber detection system, which comprises an imaging device, a plurality of branch image-transmitting optical fibers and an axial distributed optical fiber probe, wherein the axial distributed optical fiber probe is connected to one end of each branch image-transmitting optical fiber and is used for detecting the end face and the axial side faces with different heights of a target; the axial distributed fiber optic probe is the probe device of any one of the above.
Further, referring to fig. 13, a schematic structural diagram of an embodiment of the optical fiber detection system of the present invention is shown; the optical fiber detection system comprises a CMOS image sensor 3, an achromatic lens 4, an optical filter 5, a second light path conversion mirror 6, a second light source 7, an objective lens 8, an image transmission optical fiber bundle 9, a first light path conversion mirror 10, a DMD module 11, a first light source 12 and a branch image transmission optical fiber 13; the second light source is an excitation light source, is connected to the objective lens 8 through the second light path conversion mirror 6, and is connected with a target to be detected through an optical fiber for detection so as to form an excitation light channel for light emitted by the excitation light source to pass through; the objective lens is connected to the CMOS image sensor 3 through the second light path conversion mirror 6, the first light path conversion mirror 10, the optical filter 5 and the achromatic lens 4 to form an imaging light channel for passing fluorescence emitted by an imaging target; furthermore, an axial distributed optical fiber probe which is arranged at one end of the optical fiber and used for detection is a probe device in the invention, a plurality of branch image transmission optical fibers 13 and a probe device which is connected with one end of each branch image transmission optical fiber and used for detecting the end face and axial different height side faces of a target, the other end of each branch image transmission optical fiber is bundled together to form an image transmission optical fiber bundle 9, and the end face of the optical fiber bundle is positioned on the focal plane of the objective lens; when multi-level detection is required to be carried out on different parts at the same time, a plurality of branch image transmission optical fibers can be arranged at the same time, and one end of each branch image transmission optical fiber is used for detection and is a probe device; a plurality of axially distributed fiber optic probes form an array and a multiple brain region. The branch image transmission optical fiber can be the extension of the axial distributed optical fiber probe, the microwire optical fiber is continuous, and different independent optical fiber bundles can be aligned and connected.
Furthermore, when multi-level detection is only needed to be performed on one part, only one branch optical fiber bundle needs to be arranged, namely multi-level axial resolution detection on a certain part can be realized only by arranging one probe device.
Further, the probe device is used for forming a light genetic light regulation and control and optical fiber recording system with axial resolution capability. The system can be cut into a system only for optogenetic or optical fiber recording, can be reduced from a multi-point array to a single point, and can be made into a miniaturized system and then directly fixed on the head of a freely moving animal for complex behavioural research.
Further, the DMD module is a Digital Micromirror Device (DMD), which is one of the optical switches, and the DMD module is configured to open and close the optical switch by using a rotating mirror, and the opening and closing time is slightly longer, and is in the order of microseconds. The action process is very simple, light comes out of the optical fiber and is emitted to a reflecting mirror of the DMD, and when the DMD is opened, the light can enter the optical fiber at the other end through a symmetrical light path; when the DMD is turned off, namely the reflector of the DMD rotates slightly, light cannot enter the other symmetrical end after being reflected, and the effect of turning off the optical switch is achieved; thereby enabling the shape, size, location, and number of DMD illuminations to be freely customized. The DMD is a chip applied to a projector, which can control the intensity and duration of light of each pixel, which means that an operator can stimulate a dozen neurons without affecting other cells, and is programmable, so that the operator can precisely control the intensity and wavelength of light of each pixel, and even make precise control of each cell.
Furthermore, the image transmission optical fiber bundle is a passive device capable of arbitrarily bending and transmitting images, and is formed by regularly arranging a plurality of optical fibers into a bundle, each optical fiber is a pixel and can independently transmit information, crosstalk does not exist among the optical fibers, and compared with a traditional optical imaging device, the image transmission optical fiber bundle has the advantages of light weight, convenience in use, convenience in carrying and the like, so that the image transmission optical fiber bundle is applied to the fields of medicine, military, aerospace, scientific research and the like, particularly has radiation resistance, corrosion resistance and electromagnetic interference resistance, and is not replaceable in some special application occasions. One feature of the optical fiber image transmission bundle is that the image can be divided, combined and transformed by different arrangements of optical fibers, for example, one end of the optical fiber bundle is in a circular or square arrangement, and the other end of the optical fiber bundle is in a straight line arrangement, so that a two-dimensional image is expanded into one dimension, and therefore, the optical fiber image transmission bundle has some unique applications.
Specifically, the arrangement mode of the optical fibers/optical fiber bundles adopted in the axially distributed optical fiber probe in the present invention is any optical fiber arrangement mode provided in the second aspect of the present invention, and the probe with the required axial resolution capability is formed only by arranging and fixing a plurality of optical fibers/optical fiber bundles without external limitation of the probe device, so as to detect the target to be detected.
Further, the implementation method of the axially distributed image transmission fiber bundle is as follows: closely arranging the microwire optical fibers on a thin flexible plane in a single layer, adhering and fixing the microwire optical fibers, aligning one end of each microwire optical fiber with the plane, distributing the other end of each microwire optical fiber on different depths, and rolling up the plane to form an axially-distributed image transmission optical fiber bundle; or drawing the planar optical waveguide on a plane by means of 3D printing, and then rolling the plane to form the axially distributed image transmission optical fiber bundle.
Furthermore, when one through hole for penetrating the optical fiber is arranged in the probe device body, one end of the probe device is of a pointed structure, and the other end of the probe device is of a through hole; the cross section of the optical fiber at the tip structure leakage part is consistent with the cross section structure of the through hole at the tip structure, namely the end face arranged at the tip structure can penetrate through the detection light of the optical fiber bundle in the through hole; when the body is internally provided with a through hole, the optical fibers arranged in the body are axially distributed image transmission optical fiber bundles, one end of each axially distributed image transmission optical fiber bundle used for realizing axial resolution detection is matched with the end part and the sealing part of the tip structure of the probe device, and the optical fibers penetrating through the axially distributed image transmission optical fiber bundles are ensured to realize axial detection.
Further, the optical fiber detection system comprises an optical fiber nerve regulation and recording system.
While the invention has been described with reference to a preferred embodiment, various changes may be made and equivalents may be substituted for elements thereof without departing from the scope of the invention, especially if structural conflict does not exist and the technical features mentioned in the various embodiments may be combined in any way; it is intended that the invention not be limited to the particular embodiments disclosed, but that the invention will include all embodiments falling within the scope of the appended claims.
In the description of the present invention, the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like, which indicate directions or positional relationships, are based on the directions or positional relationships shown in the drawings, which are for convenience of description only, and do not indicate or imply that the devices or elements must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
Furthermore, it should be noted that, in the description of the present invention, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
The terms "comprises," "comprising," or any other similar term are intended to cover a non-exclusive inclusion, such that a process, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, article, or apparatus.
So far, the technical solutions of the present invention have been described in connection with the preferred embodiments shown in the drawings, but it is easily understood by those skilled in the art that the scope of the present invention is obviously not limited to these specific embodiments. Equivalent changes or substitutions of related technical features can be made by those skilled in the art without departing from the principle of the invention, and the technical scheme after the changes or substitutions can fall into the protection scope of the invention.
Claims (8)
1. A probe device is characterized by comprising a body and an optical fiber; one end of the body is of a pointed structure, and one or more through holes for the optical fibers to penetrate through are formed in the body; when the number of the through holes is multiple, the through holes are arranged in parallel along the length direction of the body; the cross section of the optical fiber at the tip structure leakage part is consistent with the cross section structure of the through hole at the tip structure;
the body comprises a first part and a second part, wherein one end of the first part is used for being connected with a set installation part, and the other end of the first part is connected with one end of the second part; the second component comprises one or more ends having a first cross-sectional area, and one or more seals having a second cross-sectional area; a first cross-sectional area of the end portion is greater than a second cross-sectional area of the adjacent sealing portion in an implanted length direction of the body;
the second component comprises a first working surface and a second working surface, and the first working surface and the second working surface are respectively arranged on two sides of the second component; the first working surface is provided with a plurality of end parts and a plurality of sealing parts; the second working surface is a smooth sealing surface; one end, far away from the first part, of the first working face is connected with the second working face, and the connecting surfaces of the first working face, the second working face and the second part are arranged in a triangular mode.
2. The probe apparatus of claim 1, wherein the adjacent end portions are arranged in a stepped-down configuration with the sealing portion.
3. The probe apparatus of claim 1, wherein adjacent ends are arranged in a fold-down arrangement.
4. The probe apparatus of claim 1, wherein adjacent said ends are arranged in a spiral descending manner;
the first component is arranged in a cylindrical shape; the second part is arranged in a conical shape.
5. The probe device of claim 1, wherein the first member is prism-shaped;
the second part is arranged in a pyramid shape; a plurality of pyramid sides of the second member are each provided with a plurality of the end portions and a plurality of the sealing portions; the end face of the end part and the end face of the sealing part are both inclined cutting faces.
6. The probe apparatus of claim 1, wherein the first member is cylindrically shaped;
the second part is arranged in a conical shape; the conical side surfaces of the second component are provided with a plurality of end parts and a plurality of sealing parts; the end face of the end part and the end face of the sealing part are both inclined cutting faces.
7. The probe apparatus of claim 1, wherein the first member and the second member are integrally formed or fixedly attached.
8. An optical fiber detection system is characterized by comprising an imaging device, a plurality of branch image transmission optical fibers and an axial distributed optical fiber probe which is connected to one end of each branch image transmission optical fiber and is used for detecting the end face and the axial side faces with different heights of a target, wherein the other ends of the branch image transmission optical fibers are bundled together to form an optical fiber bundle, and the end face of the optical fiber bundle is positioned on the focal plane of the imaging device; the axially distributed fiber optic probe is the probe device of any one of claims 1-7.
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| WO1997034175A1 (en) * | 1996-03-13 | 1997-09-18 | Visionex, Inc. | Method and apparatus for improved fiber optic light management |
| JP2005118429A (en) * | 2003-10-20 | 2005-05-12 | Pentax Corp | Very small diameter endoscope |
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|---|---|---|---|---|
| US9295391B1 (en) * | 2000-11-10 | 2016-03-29 | The General Hospital Corporation | Spectrally encoded miniature endoscopic imaging probe |
| GB2570252B (en) * | 2016-10-14 | 2021-11-03 | Synaptive Medical Inc | Micrometer size multi-functional probe for OCT and electro-physiological recording |
| WO2018113887A2 (en) * | 2016-12-20 | 2018-06-28 | 3Dintegrated Aps | A medical probe assembly |
| IT201700028787A1 (en) * | 2017-03-15 | 2018-09-15 | Fondazione St Italiano Tecnologia | System and method for collecting light axially resolved through a tapered waveguide. |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997034175A1 (en) * | 1996-03-13 | 1997-09-18 | Visionex, Inc. | Method and apparatus for improved fiber optic light management |
| JP2005118429A (en) * | 2003-10-20 | 2005-05-12 | Pentax Corp | Very small diameter endoscope |
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