CN113718006B - Oyster peptide extraction method - Google Patents
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- 241000237502 Ostreidae Species 0.000 title claims abstract description 42
- 235000020636 oyster Nutrition 0.000 title claims abstract description 42
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 14
- 238000000605 extraction Methods 0.000 title abstract description 9
- 239000006185 dispersion Substances 0.000 claims abstract description 43
- 239000007788 liquid Substances 0.000 claims abstract description 34
- 229920000168 Microcrystalline cellulose Polymers 0.000 claims abstract description 33
- 235000019813 microcrystalline cellulose Nutrition 0.000 claims abstract description 33
- 239000008108 microcrystalline cellulose Substances 0.000 claims abstract description 33
- 229940016286 microcrystalline cellulose Drugs 0.000 claims abstract description 33
- 239000000843 powder Substances 0.000 claims abstract description 27
- 229910001385 heavy metal Inorganic materials 0.000 claims abstract description 20
- 238000003756 stirring Methods 0.000 claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- NKWPZUCBCARRDP-UHFFFAOYSA-L calcium bicarbonate Chemical compound [Ca+2].OC([O-])=O.OC([O-])=O NKWPZUCBCARRDP-UHFFFAOYSA-L 0.000 claims abstract description 14
- 229910000020 calcium bicarbonate Inorganic materials 0.000 claims abstract description 14
- 102000004190 Enzymes Human genes 0.000 claims abstract description 12
- 108090000790 Enzymes Proteins 0.000 claims abstract description 12
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 claims abstract description 12
- 238000001914 filtration Methods 0.000 claims abstract description 12
- LXAHHHIGZXPRKQ-UHFFFAOYSA-N 5-fluoro-2-methylpyridine Chemical compound CC1=CC=C(F)C=N1 LXAHHHIGZXPRKQ-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000000706 filtrate Substances 0.000 claims abstract description 6
- 229910052751 metal Inorganic materials 0.000 claims abstract description 5
- 239000002184 metal Substances 0.000 claims abstract description 5
- 238000002156 mixing Methods 0.000 claims abstract description 5
- 238000004806 packaging method and process Methods 0.000 claims abstract description 5
- 238000010298 pulverizing process Methods 0.000 claims abstract description 5
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 39
- 238000010438 heat treatment Methods 0.000 claims description 18
- 108090000145 Bacillolysin Proteins 0.000 claims description 11
- 102000035092 Neutral proteases Human genes 0.000 claims description 11
- 108091005507 Neutral proteases Proteins 0.000 claims description 11
- 239000004367 Lipase Substances 0.000 claims description 10
- 102000004882 Lipase Human genes 0.000 claims description 10
- 108090001060 Lipase Proteins 0.000 claims description 10
- 235000019421 lipase Nutrition 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 9
- 230000001105 regulatory effect Effects 0.000 claims description 9
- 238000001694 spray drying Methods 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 abstract description 10
- 108090000623 proteins and genes Proteins 0.000 abstract description 10
- 238000011084 recovery Methods 0.000 abstract description 10
- 230000008901 benefit Effects 0.000 abstract description 3
- 238000001035 drying Methods 0.000 abstract description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 14
- 239000000047 product Substances 0.000 description 11
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 9
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 7
- 235000017557 sodium bicarbonate Nutrition 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 4
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 description 4
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 4
- 229910052793 cadmium Inorganic materials 0.000 description 4
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 4
- 229910052804 chromium Inorganic materials 0.000 description 4
- 239000011651 chromium Substances 0.000 description 4
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 description 4
- 102000004196 processed proteins & peptides Human genes 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 235000019750 Crude protein Nutrition 0.000 description 3
- 230000009920 chelation Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 229920001184 polypeptide Polymers 0.000 description 3
- 238000000967 suction filtration Methods 0.000 description 3
- 238000003828 vacuum filtration Methods 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 239000000839 emulsion Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- VCUFZILGIRCDQQ-KRWDZBQOSA-N N-[[(5S)-2-oxo-3-(2-oxo-3H-1,3-benzoxazol-6-yl)-1,3-oxazolidin-5-yl]methyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C1O[C@H](CN1C1=CC2=C(NC(O2)=O)C=C1)CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F VCUFZILGIRCDQQ-KRWDZBQOSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 235000020247 cow milk Nutrition 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000004251 human milk Anatomy 0.000 description 1
- 235000020256 human milk Nutrition 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 230000006916 protein interaction Effects 0.000 description 1
- 235000014102 seafood Nutrition 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Water Supply & Treatment (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The application discloses an oyster peptide extraction method, which comprises the following steps: (1) crushing: pulverizing cleaned and dried Concha Ostreae and Carnis Ostreae into Concha Ostreae fine powder; (2) dispersing: mixing oyster fine powder, calcium bicarbonate, sodium bicarbonate, microcrystalline cellulose and water according to a mass ratio of 1:0.02-0.04:0.04-0.06:0.01-0.03:23-28, stirring, and uniformly dispersing to obtain a dispersion liquid; (3) enzymolysis: adding a decomposing enzyme into the dispersion liquid to carry out enzymolysis on the dispersion liquid; (4) heavy metal removal: adding EDTA disodium into the dispersion liquid after enzymolysis, and stirring and reacting for 25-35 min; (5) filtering: filtering the dispersion liquid after metal removal, and taking filtrate; (6) concentrating, drying and packaging; has the advantages of removing heavy metals and improving the recovery rate of protein.
Description
Technical Field
The application relates to an oyster peptide extraction method.
Background
The sea food oyster has huge edible value and medicinal value, is the first large cultured shellfish in the world, and is cultured on a large scale in coastal areas such as Zhejiang province in China. Zhejiang Ninghaiqian is known as "oyster village", and there is 700 years of oyster cultivation history. The nutrition value of the oyster is extremely high, the protein content in the oyster is up to 45% -57%, and the amino acid composition is perfect. According to the assessment of the world grain and agriculture organization, the degree of completeness and mass proportion of essential amino acids in oyster meat are superior to those of human milk and cow milk. Ancient people called oyster the most noble of aquatic products, ancient romans had praised it as "sea delicacy-holy fish", western people as "goddess magic stone", "sea milk", and Japanese people as "root source".
Due to marine pollution, considerable heavy metals exist in modern marine organisms such as oysters, so that the technical problem needs to be considered when the oysters are treated.
Disclosure of Invention
The application aims to provide an oyster peptide extraction method which has the advantage of removing heavy metals.
The technical aim of the application is realized by the following technical scheme:
an oyster peptide extraction method comprises the following steps:
(1) Crushing: pulverizing cleaned and dried Concha Ostreae and Carnis Ostreae into Concha Ostreae fine powder;
(2) Dispersing: mixing oyster fine powder, calcium bicarbonate, sodium bicarbonate, microcrystalline cellulose and water, stirring, and dispersing uniformly to obtain a dispersion liquid; the input mass ratio of the oyster fine powder, the calcium bicarbonate, the sodium bicarbonate, the microcrystalline cellulose and the water is 1:0.02-0.04:0.04-0.06:0.01-0.03:23-28;
(3) Enzymolysis: adding a decomposing enzyme into the dispersion liquid to carry out enzymolysis on the dispersion liquid;
(4) Heavy metal removal: adding EDTA disodium into the dispersion liquid after enzymolysis, and stirring and reacting for 25-35 min;
(5) And (3) filtering: filtering the dispersion liquid after metal removal, and taking filtrate;
(6) Concentrating, drying, and packaging.
Further, in the step (3), the decomposing enzyme comprises neutral protease and lipase, and the enzymolysis step comprises the following two parts:
(1) regulating pH of the dispersion to 6.8-7.2, heating to 50-55deg.C, adding neutral protease, stirring for enzymolysis for 5-7 hr, heating to 75-85deg.C, and maintaining for 8-12 min to inactivate enzyme; the mass ratio of the dispersion liquid to the neutral protease is 100:0.2-0.4;
(2) regulating pH of the dispersion to 6.8-7.2, heating to 38-42 ℃, adding lipase, stirring for enzymolysis for 2-4 hr, heating to 85-95 ℃ after enzymolysis, and preserving heat for 4-5 min to inactivate enzyme; the mass ratio of the dispersion liquid to the lipase is 100:0.1-0.3.
Further, in the step (4), microcrystalline cellulose and ethyl acetate are added to the dispersion in addition to disodium EDTA; in the step (4), the mass ratio of the dispersion liquid after enzymolysis to the EDTA disodium, the microcrystalline cellulose and the ethyl acetate is 100:0.02-0.04:0.08-0.11:7-9.
Further, the method comprises the following steps:
(1) Crushing: pulverizing cleaned and dried Concha Ostreae and Carnis Ostreae into Concha Ostreae fine powder;
(2) Dispersing: mixing oyster fine powder, calcium bicarbonate, sodium bicarbonate, microcrystalline cellulose and water, stirring, and dispersing uniformly to obtain a dispersion liquid; the input mass ratio of the oyster fine powder, the calcium bicarbonate, the sodium bicarbonate, the microcrystalline cellulose and the water is 1:0.03:0.05:0.02:25;
(3) Enzymolysis:
(1) regulating pH of the dispersion to 7.0, heating to 52deg.C, adding neutral protease, stirring for enzymolysis for 6hr, heating to 80deg.C, and maintaining for 10min to deactivate enzyme; the mass ratio of the dispersion liquid to the neutral protease is 100:0.3;
(2) regulating pH of the dispersion to 7.0, heating to 40deg.C, adding lipase, stirring for enzymolysis for 3hr, heating to 90deg.C, and maintaining for 5min to deactivate enzyme; the mass ratio of the dispersion liquid to the lipase is 100:0.2;
(4) Heavy metal removal: adding EDTA disodium, microcrystalline cellulose and ethyl acetate into the dispersion liquid after enzymolysis, and stirring and reacting for 30min; in the step (4), the mass ratio of the dispersion liquid after enzymolysis to EDTA disodium, microcrystalline cellulose and ethyl acetate is 100:0.03:0.10:8;
(5) And (3) filtering: vacuum filtering the dispersion liquid after metal removal with a 0.5 μm filter membrane, and collecting filtrate;
(6) Concentrating under vacuum until the solid content is 50wt%, spray drying, and packaging.
The technical effects of the application are mainly as follows:
in the oyster processing process, EDTA disodium is used for heavy metal processing, so that the heavy metal content in the product is reduced, and the food safety is improved; in the treatment process, because of the existence of heavy metals, the possibility of chelation exists between the polypeptides and the heavy metals in the process, so that the chelated heavy metals exist in the product, the calcium bicarbonate is utilized to push and combine microcrystalline cellulose to disperse and block chelation under alkaline conditions and the action of the heavy metals, so that the chelation of the heavy metals and the polypeptides is reduced, the heavy metals are in a free state, so that the heavy metals act with EDTA disodium, the heavy metal removal effect is improved, and the loss of the polypeptides is also reduced;
the method has the advantages that the emulsion stability in the enzymolysis liquid obtained after oyster enzymolysis is good, the separation is not ideal, the separation time and the product quality are influenced, the interaction between protein and fat is destroyed by lipase enzymolysis, and the stability of emulsion is reduced, so that the oil-water separation is realized, the separation effect is improved, the process progress is accelerated, and the protein recovery rate is improved by the action of ethyl acetate and microcrystalline cellulose.
Detailed Description
Example 1: an oyster peptide extraction method comprises the following steps:
(1) Crushing: pulverizing cleaned and dried Concha Ostreae and Carnis Ostreae into Concha Ostreae fine powder;
(2) Dispersing: mixing oyster fine powder, calcium bicarbonate, sodium bicarbonate, microcrystalline cellulose and water, stirring, and dispersing uniformly to obtain a dispersion liquid; the input mass ratio of the oyster fine powder, the calcium bicarbonate, the sodium bicarbonate, the microcrystalline cellulose and the water is 1:0.03:0.05:0.02:25;
(3) Enzymolysis:
(1) regulating pH of the dispersion to 7.0, heating to 52deg.C, adding neutral protease, stirring for enzymolysis for 6hr, heating to 80deg.C, and maintaining for 10min to deactivate enzyme; the mass ratio of the dispersion liquid to the neutral protease is 100:0.3;
(2) regulating pH of the dispersion to 7.0, heating to 40deg.C, adding lipase, stirring for enzymolysis for 3hr, heating to 90deg.C, and maintaining for 5min to deactivate enzyme; the mass ratio of the dispersion liquid to the lipase is 100:0.2;
(4) Heavy metal removal: adding EDTA disodium, microcrystalline cellulose and ethyl acetate into the dispersion liquid after enzymolysis, and stirring and reacting for 30min; in the step (4), the mass ratio of the dispersion liquid after enzymolysis to EDTA disodium, microcrystalline cellulose and ethyl acetate is 100:0.03:0.10:8;
(5) And (3) filtering: vacuum filtering the dispersion liquid after metal removal with a 0.5 μm filter membrane, and collecting filtrate;
(6) Concentrating under vacuum until the solid content is 50wt%, spray drying, and packaging.
Example 1 oyster peptides have a molecular weight distribution as shown in table 1.
TABLE 1
Molecular weight range (Dalton) | Percent peak area (%,. Lambda.220 nm) |
M>3000 | 0.15 |
2000<M≤3000 | 0.44 |
1000<M≤2000 | 2.03 |
180<M≤1000 | 17.25 |
M≤180 | 80.13 |
Example 2: influence of step (2) on oyster peptide extraction method
Test object: example 1, control 1-5.
Control group 1: referring to example 1, the difference from example 1 is that the input mass ratio of oyster fine powder, monocalcium carbonate, sodium bicarbonate, microcrystalline cellulose and water in step (2) is 1:0.02:0.04:0.01:25.
Control group 2: referring to example 1, the difference from example 1 is that the input mass ratio of oyster fine powder, monocalcium carbonate, sodium bicarbonate, microcrystalline cellulose and water in step (2) is 1:0.04:0.06:0.03:25.
Control group 3: referring to example 1, the difference from example 1 is that no monocalcium carbonate, sodium bicarbonate or microcrystalline cellulose was added to the oyster powder in step (2), only water was added and the input mass ratio of the oyster powder to the water was 1:25.
Control group 4: referring to example 1, the difference from example 1 is that microcrystalline cellulose was not added to the oyster shell fine powder in step (2), and the input mass ratio of the oyster shell fine powder, the monocalcium carbonate, the sodium bicarbonate and the water was 1:0.03:0.05:25.
Control group 5: referring to example 1, the difference from example 1 is that the input mass ratio of oyster fine powder, monocalcium carbonate, sodium bicarbonate, microcrystalline cellulose and water in step (2) is 1:0.06:0.08:0.05:25.
The test content is as follows: and (3) respectively measuring crude proteins of the test objects and measuring total amounts of three heavy metals including lead, cadmium and chromium.
Measurement of crude protein: the Kjeldahl nitrogen determination method is adopted, and the standard is GB5009.5-2010. Protein recovery (%) = product protein content x 100%/raw material protein content, product protein content is the protein content in the product obtained after spray drying in step (6), and raw material protein content is the protein content of the oyster fine powder obtained in step (1).
Heavy metal determination: referring to GB15618-1995, the test object is the product obtained after spray drying in step (6).
The test results are shown in Table 2. Table 2 shows:
(1) Referring to the data of protein recovery rate, as the contents of the oyster fine powder, the calcium bicarbonate, the sodium bicarbonate and the microcrystalline cellulose in the step (2) are increased, the protein recovery rate tends to be increased firstly, then gradually decreased; oyster fine powder, calcium bicarbonate, sodium bicarbonate and microcrystalline cellulose=1:0.03:0.05:0.02 and 1:0.04:0.06:0.03, and the protein recovery rate is more than 85%;
(2) Referring to the total amount data of lead, cadmium and chromium, along with the increase of the contents of the calcium carbonate, the sodium bicarbonate and the microcrystalline cellulose in the oyster fine powder, the calcium bicarbonate, the sodium bicarbonate and the microcrystalline cellulose in the step (2), the total amount of the lead, the cadmium and the chromium shows a trend of decreasing firstly and then increasing; oyster fine powder, calcium bicarbonate, sodium bicarbonate and microcrystalline cellulose=1:0.03:0.05:0.02, and the total amount of lead, cadmium and chromium is 0.
TABLE 2
Example 3: influence of step (3) and step (4) on oyster peptide extraction method
Test object: example 1, control 6-9.
Control group 6: referring to example 1, the difference from example 1 is that the enzymatic hydrolysis step (3) includes only one of the following: regulating pH of the dispersion to 7.0, heating to 52deg.C, adding neutral protease, stirring for enzymolysis for 6hr, heating to 80deg.C, and maintaining for 10min to deactivate enzyme; the mass ratio of the dispersion liquid and the neutral protease is 100:0.3.
Control group 7: referring to example 1, the difference from example 1 is that only disodium EDTA was added in step (4), and microcrystalline cellulose or ethyl acetate was not added.
Control group 8: referring to example 1, the difference from example 1 is that only disodium EDTA and microcrystalline cellulose were added in step (4), and ethyl acetate was not added.
Control group 9: referring to example 1, the difference from example 1 is that only disodium EDTA and ethyl acetate were added in step (4), and microcrystalline cellulose was not added.
The test content is as follows: and (3) respectively measuring the time consumption of vacuum filtration of the test object, namely taking 1kg of the dispersion liquid treated in the step (4), performing vacuum filtration by using a 0.5 mu m filter membrane, wherein the suction filtration pressure is 0.1MPa, the suction filtration temperature is 25 ℃, counting time from the beginning of the suction filtration, and recording the total time consumption until the filtrate is completely filtered.
Measurement of crude protein: the Kjeldahl nitrogen determination method is adopted, and the standard is GB5009.5-2010. Protein recovery (%) = product protein content x 100%/raw material protein content, product protein content is the protein content in the product obtained after spray drying in step (6), and raw material protein content is the protein content of the oyster fine powder obtained in step (1).
The test results are shown in Table 3. Table 3 shows: compared with the control group 6 which is not subjected to the two-step treatment in the step (3), the vacuumized filtration in the embodiment 1 of the application consumes more time and has higher protein recovery rate; compared with the control group 7-9 without ethyl acetate and/or microcrystalline cellulose added in step (4), the vacuuming filtration of example 1 of the present application takes more time and the protein recovery rate is higher.
TABLE 3 Table 3
Vacuum filtration time-consuming (min) | Protein recovery (%) | |
Example 1 | 10 | 85.2 |
Control group 6 | 92 | 63.0 |
Control group 7 | 53 | 70.2 |
Control group 8 | 88 | 68.1 |
Control group 9 | 82 | 66.4 |
Of course, the above is only a typical example of the application, and other embodiments of the application are also possible, and all technical solutions formed by equivalent substitution or equivalent transformation fall within the scope of the application claimed.
Claims (1)
1. The oyster peptide extracting process includes the following steps:
(1) Crushing: pulverizing cleaned and dried Concha Ostreae and Carnis Ostreae into Concha Ostreae fine powder;
(2) Dispersing: mixing oyster fine powder, calcium bicarbonate, sodium bicarbonate, microcrystalline cellulose and water, stirring, and dispersing uniformly to obtain a dispersion liquid; the input mass ratio of the oyster fine powder, the calcium bicarbonate, the sodium bicarbonate, the microcrystalline cellulose and the water is 1:0.03:0.05:0.02:25;
(3) Enzymolysis:
(1) regulating pH of the dispersion to 7.0, heating to 52deg.C, adding neutral protease, stirring for enzymolysis for 6hr, heating to 80deg.C, and maintaining for 10min to deactivate enzyme; the mass ratio of the dispersion liquid to the neutral protease is 100:0.3;
(2) regulating pH of the dispersion to 7.0, heating to 40deg.C, adding lipase, stirring for enzymolysis for 3hr, heating to 90deg.C, and maintaining for 5min to deactivate enzyme; the mass ratio of the dispersion liquid to the lipase is 100:0.2;
(4) Heavy metal removal: adding EDTA disodium, microcrystalline cellulose and ethyl acetate into the dispersion liquid after enzymolysis, and stirring and reacting for 30min; in the step (4), the mass ratio of the dispersion liquid after enzymolysis to EDTA disodium, microcrystalline cellulose and ethyl acetate is 100:0.03:0.10:8;
(5) And (3) filtering: vacuum filtering the dispersion liquid after metal removal with a 0.5 μm filter membrane, and collecting filtrate;
(6) Concentrating under vacuum until the solid content is 50wt%, spray drying, and packaging.
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