CN113717836A - Laboratory microbial cultivation device - Google Patents
Laboratory microbial cultivation device Download PDFInfo
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- CN113717836A CN113717836A CN202111058937.0A CN202111058937A CN113717836A CN 113717836 A CN113717836 A CN 113717836A CN 202111058937 A CN202111058937 A CN 202111058937A CN 113717836 A CN113717836 A CN 113717836A
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- 230000000813 microbial effect Effects 0.000 title claims description 4
- 244000005700 microbiome Species 0.000 claims abstract description 43
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 17
- 229920000742 Cotton Polymers 0.000 claims abstract description 13
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 7
- 230000005540 biological transmission Effects 0.000 claims description 33
- 239000003365 glass fiber Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 3
- 230000000712 assembly Effects 0.000 claims 2
- 238000000429 assembly Methods 0.000 claims 2
- 238000009423 ventilation Methods 0.000 abstract description 9
- 238000000034 method Methods 0.000 description 9
- 239000000428 dust Substances 0.000 description 7
- 239000012535 impurity Substances 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- 230000000694 effects Effects 0.000 description 6
- 230000009471 action Effects 0.000 description 5
- 238000012258 culturing Methods 0.000 description 5
- 238000001914 filtration Methods 0.000 description 5
- 230000005484 gravity Effects 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
- 230000008859 change Effects 0.000 description 3
- 238000009629 microbiological culture Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 208000035473 Communicable disease Diseases 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 230000001174 ascending effect Effects 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/18—Flow directing inserts
- C12M27/20—Baffles; Ribs; Ribbons; Auger vanes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/24—Recirculation of gas
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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Abstract
The invention discloses a laboratory microorganism culture device, and relates to the technical field of microorganism culture. The air-out device comprises a box body, an upper box cover and a lower box bottom which are respectively and fixedly arranged on the box body, wherein a placing component is movably arranged in the box body, an air-out component is arranged right below the placing component, and the air-out component penetrates through the lower box bottom and is fixedly connected with a circulating component in the lower box bottom; a fresh air component is fixedly arranged in the upper box cover and is fixedly connected with the circulating component; the active carbon layers and the filter cotton are respectively provided with two groups and are distributed in the filter box in a mutually staggered way; the circulation assembly comprises an air outlet baffle and an air duct, the air duct is fixedly mounted at the back of the box body, an air outlet is formed in the back of the box body, the air outlet is fixedly connected with the air outlet baffle inside, and a thirty-degree included angle is formed between the air outlet baffle and the air outlet. According to the invention, the culture dish is arranged in the placing frame, so that the dish cover of the culture dish can be ensured to be consistent with the direction of the wind all the time, and the risk of polluting the culture dish due to ventilation is reduced.
Description
Technical Field
The invention belongs to the technical field of microbial culture, and particularly relates to a laboratory microbial culture device.
Background
The microorganism is a general term for all microorganisms which are difficult to be seen by naked eyes and can be observed only by an optical microscope or an electron microscope, the microorganism has different functions according to different types, infectious diseases caused by the microorganism occupy an important part in human history, modern biology must know the growth and the propagation of the microorganism in order to solve the infectious diseases, the microorganism needs to be cultured, most of the microorganism culture is carried out in a laboratory, a microorganism incubator is required, and is important in the microorganism culture because the interference of the external environment has great influence on the result of the microorganism culture, whether the quantity, quality, performance, precision and the like of the culture box meet the requirements of culture or not is related to whether the culture can normally run, and the principle of the microorganism incubator is a device for culturing microorganisms in vitro by simulating the growth environment of the microorganisms in organisms in the incubator body.
Most of the existing microorganism incubators are provided with an air duct and a circulating system inside a box body, proper air speed and air direction are favorable for the uniformity of incubator temperature, and the normal growth of cultures is facilitated, but some existing microorganism incubators cannot adjust the air speed, and when the air speed is too high, the drying crack of a culture medium is easily caused, so that culture results are inaccurate, in addition, the culture dish is required to be in upright culture in GB4789.15-2016 (national food safety Standard food microbiology inspection mould and Yeast count) and the culture dish is required to be in inverted culture in GB4789.15-2010 (national food safety Standard food microbiology inspection mould and Yeast count) and the verification of a blank culture dish for many times shows that the same air speed is obtained, when the air direction of the incubator in operation is opposite to the orientation of a culture dish cover, the culture dish is easily polluted by dust, sundry bacteria and the like in the air, therefore, draw the conclusion that a culture dish was placed, the wind direction when the incubator ran is best unanimous with the orientation of culture dish lid, and most culture dishes are just putting when placing the in-process, has been the custom of making a appointment, but its ventilation wind direction of traditional incubator is various, and is inconsistent with culture dish lid orientation basically, has leaded to the risk increase that the culture received dust interference in the culture dish like this.
The wind direction of its ventilation system of current incubator in the process of cultivateing can not be unanimous with the ware lid orientation of culture dish, leads to cultivateing inaccurate to the too fast or with the long-time closely contact of air inlet of culture in-process wind speed leads to the microorganism in the culture dish to dry and split, dust impurity in the air also causes just so big influence to the microorganism cultivation, therefore we propose a laboratory microorganism culture apparatus.
Disclosure of Invention
The invention aims to provide a laboratory microorganism culture device, which solves the problems that the wind direction of a ventilation system of the conventional culture box in the culture process cannot be consistent with the direction of a dish cover of a culture dish, so that the culture is inaccurate, microorganisms in the culture dish are dried and cracked due to the excessively high wind speed or the long-time close contact with an air inlet in the culture process, and dust impurities in the air cause great influence on the culture of the microorganisms.
In order to solve the technical problems, the invention is realized by the following technical scheme:
the invention relates to a laboratory microorganism culture device, which comprises a box body, an upper box cover and a lower box bottom, wherein the upper box cover and the lower box bottom are respectively and fixedly arranged on the box body; the fresh air component is fixedly arranged in the upper box cover and fixedly connected with the circulating component, the interior of the box body is used for culturing microorganisms and serves as a main environment for culturing the microorganisms, and the upper box cover and the lower box bottom are used for accommodating various component parts;
the placing assembly comprises a left fixing ring, a right fixing ring, a connecting rod, a hook and a placing frame, the left fixing ring and the right fixing ring are respectively and movably connected to two opposite inner walls in the box body, the left fixing ring and the right fixing ring are fixedly connected through four equally-spaced connecting rods, two hooks are respectively and movably connected to two ends of the peripheral side surface of the connecting rod, the two hooks are respectively and fixedly connected to two ends of the top of the placing frame, the left fixing ring and the right fixing ring rotate together to drive the placing frame to rotate, so that a culture dish on the placing frame can uniformly contact with circulating air, the culture dish close to the air outlet assembly is prevented from contacting with the circulating air with high air speed in a short distance for a long time, and therefore, a culture object in the culture dish is dried and cracked, the connecting rod is used for connecting the left fixing ring and the right fixing ring and bears the placing frame at the bottom of the hook, the placing frame at the bottom of the hook is hung on the connecting rod, when the rack rotates, the hook and the connecting rod rotate under the action of gravity, and the bottom of the rack is ensured to be opposite to the air outlet assembly at any time;
the fresh air component comprises a filter box, an active carbon layer and filter cotton, and the filter box is fixedly arranged in the upper box cover; the active carbon layers and the filter cotton are respectively provided with two groups and are distributed in the filter box in a mutually staggered manner, the interior of the filter box is used for filtering the fresh air pumped in, and the active carbon layers are used for filtering and adsorbing large-particle impurities and peculiar smell in the air;
the circulating assembly comprises an air outlet baffle and an air duct, the air duct is fixedly mounted at the back of the box body, an air outlet is formed in the back of the box body, the air outlet is fixedly connected with the air outlet baffle inside, an included angle of thirty degrees is formed between the air outlet baffle and the air outlet, the inclined air outlet baffle is arranged at the air outlet, so that the air can be effectively prevented from being directly sucked into the air duct, and circulating air can more slowly and uniformly enter the air duct after being blocked by the air outlet baffle;
the air outlet assembly comprises a left gear, a transmission gear, a right gear, an air outlet groove, a first air inlet baffle and a second air inlet baffle, wherein the left gear and the right gear are meshed with each other through the two transmission gears, one end of the left gear penetrates through the air outlet groove to the interior of the air outlet groove and is fixedly connected with the first air inlet baffle, the right gear penetrates through the air outlet groove to the interior of the air outlet groove and is fixedly connected with the second air inlet baffle, the first air inlet baffle and the second air inlet baffle rotate relatively, the left gear serves as a driving gear and is not only used for driving the transmission gears to rotate but also used for driving the first air inlet baffle to rotate, the transmission gears are used for driving the right gear to rotate, the left gear, the transmission gears and the right gear are identical in size, the left gear and the right gear are respectively one in number, the transmission gears are two, and therefore, under the gear meshing transmission, the rotation directions of the left gear and the right gear are opposite, the first air inlet baffle and the second air inlet baffle rotate relatively.
Preferably, place the subassembly and still include gear motor and drive element, gear motor fixed connection is inside last case lid, and closes on the filter cartridge, gear motor runs through a case lid side and drive element one end fixed connection, the drive element other end runs through the box lateral wall to inside, and with left retaining ring central point put fixed connection, the solid fixed ring in gear motor control drive element transmission left side is rotatory to drive a plurality of racks and rotate, drive element plays the transmission effect.
Preferably, the new trend subassembly still includes air exhauster and blast pipe, the mounting hole has been seted up at the upper box cover top, the inside inlet scoop fixed connection with the air exhauster of mounting hole, the supply-air outlet and the filter box one end fixed connection of air exhauster, the one end of filter box other end fixedly connected with blast pipe, the other end of blast pipe runs through inside case lid inner wall to wind channel, and inside the air exhauster was used for extracting the device with external new trend, the blast pipe was used for connecting filter box and wind channel.
Preferably, the circulating assembly further comprises an exhaust fan, a first air delivery pipe, an air pump, a second air delivery pipe and an air hopper, the exhaust fan is fixedly connected inside the air duct, and the exhaust fan is opposite to the air outlet; first air delivery pipe one end runs through bottom of the case inner wall to the inside bottom in wind channel down, the induction port of first air delivery pipe other end fixedly connected with air pump, air pump fixed connection is inside the bottom of the case down, air pump air feed mouth and second air delivery pipe one end fixed connection, the second air delivery pipe other end is four air buckets of fixedly connected with respectively, air bucket fixed connection is in air-out subassembly bottom, and the suction fan faces the air outlet just, therefore the suction fan can be with the circulated air from the air outlet through circulate in the wind channel, and first air delivery pipe is used for connecting wind channel and air pump, and the air pump is arranged in with the circulated air in the wind channel and takes out into the air bucket, and the air bucket is used for connecting the air outlet groove.
Preferably, the air-out subassembly has two sets ofly, and two relative fixed connections of air-out subassembly, the air-out subassembly still includes step motor and settles the box, step motor fixed connection is outside at the box, the step motor output runs through the box outer wall in succession and settles box and left gear fixed connection, left side gear, drive gear and right gear activity in proper order set up inside settling the box, settle box and play air duct fixed connection, step motor is used for driving left gear rotatory, settles the inside a plurality of gear that is used for settling of box, makes it can the stable meshing rotation in inside.
Preferably, every two rectangle through-holes have been seted up respectively to inside the gas outlet groove, two the inside first air inlet baffle and the second air inlet baffle of being provided with of rectangle through-hole activity respectively, every rectangle through-hole bottom fixedly connected with air hopper respectively, two rectangle through-holes of every group gas outlet groove, two vertical ascending wind of two strands are blown off to rectangle through-hole inside, change the wind speed through two air inlet baffle rotations, and the air hopper is used for the air delivery.
Preferably, the rack is four in total, and is the right angle between every two adjacent racks, every the rack bottom all just to the air-out subassembly, and the rack is inside to be used for placing the culture dish, because the action of gravity rack always bottom down just to the air-out subassembly in rotatory process, consequently the inside culture dish of rack also keeps just putting constantly.
Preferably, the included angle between first air inlet baffle and second air inlet baffle and the groove cross section of giving vent to anger is no more than ninety degrees, first air inlet baffle and second air inlet baffle through change with give vent to anger the angle between the groove cross section to wind speed when changing the ventilation, and, because relative rotation between two baffles, two streams of wind strike the fusion each other through the guide of baffle, make two streams of wind broken up, thereby can blow off uniformly.
Preferably, the material of filter pulp is glass fiber, and the filter pulp of glass fiber material can filter the impurity of tiny granule and dust in the air.
Preferably, the box body is externally hinged with a box door, the box door is opposite to the air outlet, the box door is used for closing the box body, and the box body is in a culture state after the box door is closed, so that the culture is prevented from being polluted.
The invention has the following beneficial effects:
1. according to the invention, the placing rack for placing the culture dishes is set to be rotatable, so that each culture dish in the incubator can be ensured to be uniformly contacted with circulating air, the placing rack keeps the bottom of the air outlet component opposite to the bottom all the time in the rotating process, and the dish cover of the culture dish can be ensured to be always consistent with the direction of the wind direction because the culture dish is placed in the placing rack, so that the risk of polluting the culture dish due to ventilation is reduced.
2. Compared with the traditional filter box, the filter box is internally provided with the staggered and multi-layer activated carbon layers and the filter cotton, so that dust and impurity particles with different particle sizes in the air can be filtered and adsorbed; through set up the air-out baffle of slope in the air outlet position, can avoid the suction fan directly to take out the air flue with the circulated air for it is more slowly even to get into the inside wind in wind channel.
3. According to the invention, the air outlet assembly is arranged right below the placing rack, so that the wind direction flows from bottom to top, the biological culture is more scientific, the first air inlet baffle and the second air inlet baffle are driven to rotate relatively through meshing transmission between the gears, the wind speed of the ventilation system is changed according to the rotating included angle between the first air inlet baffle and the cross section of the air outlet groove, and two wind streams are mutually impacted and fused by the guide of the baffles due to the relative rotation between the two baffles, so that the two wind streams are scattered, the wind can be uniformly blown out, the wind blown out from the bottom to the top is uniform and slow, and the influence degree of a culture dish at the bottom is reduced to the lowest.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic view of the overall structure of a laboratory microorganism culture apparatus according to the present invention;
FIG. 2 is an enlarged view taken at A of FIG. 1 in accordance with the present invention;
FIG. 3 is a schematic front view of a laboratory microorganism culture apparatus according to the present invention;
FIG. 4 is a schematic diagram of a right-side cross-sectional structure of a laboratory microorganism culture apparatus according to the present invention;
FIG. 5 is an enlarged view of the invention at B of FIG. 4;
FIG. 6 is a schematic top sectional view of a laboratory microorganism culture apparatus according to the present invention;
FIG. 7 is a schematic cross-sectional view of a filter cassette of a laboratory microorganism culture apparatus according to the present invention;
FIG. 8 is a schematic structural view of an air outlet assembly of the laboratory microorganism culture apparatus according to the present invention;
fig. 9 is a schematic left-view cross-sectional structure view of an air outlet assembly of the laboratory microbial culture apparatus of the invention.
In the drawings, the components represented by the respective reference numerals are listed below:
1. a box body; 2. a box door; 3. an upper box cover; 4. the bottom of the box; 5. a reduction motor; 6. a transmission element; 7. a left retaining ring; 8. a right securing ring; 9. a connecting rod; 10. hooking; 11. placing a rack; 12. an exhaust fan; 13. a filter cartridge; 14. an activated carbon layer; 15. filtering cotton; 16. an air supply pipe; 17. an exhaust fan; 18. an air outlet baffle plate; 19. an air duct; 20. a first air delivery pipe; 21. an air pump; 22. a second air delivery pipe; 23. an air hopper; 24. a stepping motor; 25. placing a box; 26. a left gear; 27. a transmission gear; 28. a right gear; 29. an air outlet groove; 30. a first air inlet baffle; 31. the second air inlet baffle.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front", "rear", "both ends", "one end", "the other end", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "connected," and the like are to be construed broadly, such as "connected," which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Referring to fig. 1-9, the invention is a laboratory microorganism culture device, which comprises a box body 1, an upper box cover 3 and a lower box bottom 4 which are fixedly arranged on the upper part and the lower part of the box body 1 respectively, wherein the upper box cover 3 is positioned right above the lower box bottom 4, a placing assembly is movably arranged in the box body 1, an air outlet assembly is arranged right below the placing assembly, and the air outlet assembly penetrates through the lower box bottom 4 and is fixedly connected with a circulating assembly in the lower box bottom; the interior of the upper box cover 3 is fixedly provided with a fresh air component which is fixedly connected with the circulating component, the interior of the box body 1 is used for culturing microorganisms and is used as a main environment for culturing the microorganisms, and the upper box cover 3 and the lower box bottom 4 are used for arranging various component parts; a box door 2 is hinged to the outer part of the box body 1, the box door 2 is opposite to the air outlet, the box door 2 is used for closing the box body 1, and the inner part of the box body 1 is in a culture state after the box door 2 is closed, so that the culture is prevented from being polluted;
the placing component comprises a left fixing ring 7, a right fixing ring 8, a connecting rod 9, hooks 10 and a placing rack 11, the left fixing ring 7 and the right fixing ring 8 are respectively and movably connected to two opposite inner walls in the box body 1, the left fixing ring 7 and the right fixing ring 8 are fixedly connected through four equally-spaced connecting rods 9, two peripheral side ends of the connecting rods 9 are respectively and movably connected with the hooks 10, the two hooks 10 are respectively and fixedly connected to two ends of the top of the placing rack 11, the left fixing ring 7 and the right fixing ring 8 rotate together to drive the placing rack 11 to rotate, so that a culture dish on the placing rack 11 can be ensured to be uniformly contacted with circulating air, the culture dish close to the air outlet component is prevented from being contacted with the circulating air with high air speed in a short distance for a long time, and therefore cultures in the culture dish are dried and cracked, the connecting rod 9 is used for connecting the left fixing ring 7 and the right fixing ring 8 and bears the placing rack 11 at the bottom of the hooks 10, the placing rack 11 connected with the bottom of the hook 10 is hung on the connecting rod 9, and when the placing rack rotates, the hook 10 and the connecting rod 9 rotate under the action of gravity, so that the bottom of the placing rack 11 is ensured to be opposite to the air outlet assembly at any time; the number of the placing racks 11 is four, every two adjacent placing racks 11 are in a right angle, the bottom of each placing rack 11 is opposite to the air outlet assembly, a culture dish is placed in each placing rack 11, and the bottom of each placing rack 11 always faces downwards to the air outlet assembly in the rotating process under the action of gravity, so that the culture dish in each placing rack 11 is kept upright all the time;
the fresh air component comprises a filter box 13, an activated carbon layer 14 and filter cotton 15, and the filter box 13 is fixedly arranged in the upper box cover 3; the active carbon layers 14 and the filter cotton 15 are respectively provided with two groups and are distributed in the filter box 13 in a mutually staggered manner, the filter box 13 is used for filtering the fresh air pumped in, and the active carbon layers 14 are used for filtering and adsorbing large particle impurities and peculiar smell in the air; the filter cotton 15 is made of glass fiber, and the filter cotton 15 made of glass fiber can filter small-particle impurities and dust in the air;
the circulating assembly comprises an air outlet baffle 18 and an air duct 19, the air duct 19 is fixedly mounted at the back of the box body 1, an air outlet is formed in the back of the box body 1, the air outlet is fixedly connected with the air outlet baffle 18, an included angle of thirty degrees is formed between the air outlet baffle 18 and the air outlet, the inclined air outlet baffle 18 is arranged at the air outlet, air can be effectively prevented from being directly sucked into the air duct 19, and circulating air enters the air duct 19 more slowly and uniformly after being blocked by the air outlet baffle 18;
the air outlet component comprises a left gear 26, a transmission gear 27, a right gear 28, an air outlet groove 29, a first air inlet baffle 30 and a second air inlet baffle 31, the left gear 26 and the right gear 28 are mutually meshed through the two transmission gears 27, one end of the left gear 26 penetrates through the air outlet groove 29 to the interior and is fixedly connected with the first air inlet baffle 30, the right gear 28 penetrates through the air outlet groove 29 to the interior and is fixedly connected with the second air inlet baffle 31, the first air inlet baffle 30 and the second air inlet baffle 31 rotate relatively, the left gear 26 is used as a driving gear and is not only used for driving the transmission gear 27 to rotate but also used for driving the first air inlet baffle 30 to rotate, the transmission gear 27 is used for driving the right gear 28 to rotate, the left gear 26, the transmission gear 27 and the right gear 28 are identical in size, the left gear 26 and the right gear 28 are respectively one in number, the two transmission gears 27 are arranged, therefore under the gear meshing transmission, the rotation directions of the left gear 26 and the right gear 28 are opposite, so that the first air inlet baffle 30 and the second air inlet baffle 31 rotate relatively; the included angle between the cross section of the first air inlet baffle 30 and the cross section of the second air inlet baffle 31 and the air outlet groove 29 is not more than ninety degrees, the angle between the cross section of the air outlet groove 29 is changed by the first air inlet baffle 30 and the second air inlet baffle 31, so that the air speed during ventilation is changed, and due to relative rotation between the two baffles, two air streams are mutually impacted and fused by the guide of the baffles, so that the two air streams are scattered, and therefore the air streams can be uniformly blown out.
Place the subassembly and still include gear motor 5 and transmission element 6, gear motor 5 fixed connection is inside last case lid 3, and close on filter cartridge 13, gear motor 5 runs through 3 sides of last case lid and 6 one end fixed connection of transmission element, the transmission element 6 other end runs through 1 lateral wall of box to inside, and with the solid fixed ring 7 central point of the left side put fixed connection, gear motor 5 control transmission element 6 transmission left side solid fixed ring 7 is rotatory, thereby drive a plurality of rack 11 and rotate, transmission element 6 plays the transmission effect.
The new trend subassembly still includes air exhauster 12 and blast pipe 16, the mounting hole has been seted up at 3 tops of upper box cover, the inside inlet scoop fixed connection with air exhauster 12 of mounting hole, the supply-air outlet and the 13 one end fixed connection of filter cartridge of air exhauster 12, the one end of 13 other end fixedly connected with blast pipe 16 of filter cartridge, 3 inner walls of upper box cover to 19 insides are run through to the other end of blast pipe 16, inside air exhauster 12 was used for drawing external new trend to the device, blast pipe 16 was used for connecting filter cartridge 13 and wind channel 19.
The circulating component also comprises an exhaust fan 17, a first air delivery pipe 20, an air pump 21, a second air delivery pipe 22 and an air hopper 23, wherein the exhaust fan 17 is fixedly connected inside the air duct 19, and the exhaust fan 17 is opposite to the air outlet; one end of the first air delivery pipe 20 penetrates through the inner wall of the lower box bottom 4 to the bottom end inside the air duct 19, the other end of the first air delivery pipe 20 is fixedly connected with an air suction port of an air pump 21, the air pump 21 is fixedly connected inside the lower box bottom 4, an air supply port of the air pump 21 is fixedly connected with one end of the second air delivery pipe 22, the other end of the second air delivery pipe 22 is respectively and fixedly connected with four air hoppers 23, the air hoppers 23 are fixedly connected to the bottom of the air outlet assembly, the air suction fan 17 faces the air outlet, therefore, the air suction fan 17 can suck the circulating air from the air outlet to circulate through the air duct 19, the first air delivery pipe 20 is used for connecting the air duct 19 with the air pump 21, the air pump 21 is used for sucking the circulating air in the air duct 19 into the air hoppers 23, and the air hoppers 23 are used for connecting the air outlet grooves 29.
The air-out subassembly has two sets ofly, and two relative fixed connections of air-out subassembly, the air-out subassembly still includes step motor 24 and settles box 25, step motor 24 fixed connection is outside at box 1, step motor 24 output runs through box 1 outer wall in succession and settles box 25 and left gear 26 fixed connection, left gear 26, drive gear 27 and right gear 28 activity in proper order set up inside settling box 25, settle box 25 and play air duct 29 fixed connection, step motor 24 is used for driving left gear 26 rotatory, it is inside to be used for settling a plurality of gear to settle box 25, make it can the stable meshing rotation in inside.
Two rectangle through-holes have been seted up respectively to every air outlet groove 29 inside, and two rectangle through-holes are inside to be provided with first air inlet baffle 30 and second air inlet baffle 31 respectively movably, and every rectangle through-hole bottom is fixedly connected with air hopper 23 respectively, and two inside rectangle through-holes of every air outlet groove 29 of group blow off two vertical ascending winds inside the rectangle through-hole, change the wind speed through two air inlet baffle rotations, and air hopper 23 is used for the air delivery.
The working principle of the invention is as follows: the laboratory glassware is sequentially and positively arranged in the placing frame 11 by an experimenter, the box door 2 is closed, after the circulating ventilation system is started, the speed reducing motor 5 drives the transmission element 6 to drive the left fixing ring 7 and the right fixing ring 8 to rotate, and simultaneously drives the placing frame 11 hung on the surface of the speed reducing motor to rotate, and the bottom of the placing frame 11 is always opposite to the air outlet assembly under the action of gravity in the rotating process; the air extracting fan 17 is over against the air outlet to extract air in the box body 1 into the air duct 19, the air pump 21 extracts circulating air in the air duct 19 and conveys the circulating air into the air hopper 23, so that the circulating air is vertically blown out from the air outlet groove 29 connected with the air hopper 23, the stepping motor 24 drives the left gear 26 to rotate, the right gear 28 and the left gear 26 rotate relatively under the transmission of the transmission gear 27, the first air inlet baffle 30 and the second air inlet baffle 31 which are connected with the left gear are driven to rotate relatively, the air speed of the circulating air blown out from the air outlet groove 29 is changed due to the blocking of the two air inlet baffles, and two circulating air with opposite oblique angles impact each other and are scattered and distributed in the box body 1; when fresh air needs to be replaced, the exhaust fan 12 pumps the fresh air into the device, the fresh air is filtered layer by layer through the activated carbon layer 14 and the filter cotton 15 which are staggered in the filter box 13, and then the fresh air is conveyed to the air duct 19 through the blast pipe 16 for internal circulation; the model of the speed reducing motor 5 is M2IK90N-C, the model of the exhaust fan 12 is MC-JY, the model of the air pump 21 is MC-GDJ, and the model of the stepping motor 24 is 86CME 120.
The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. The utility model provides a laboratory microbial cultivation device, includes box (1) to and respectively fixed last case lid (3) and the lower bottom of the case (4) of setting on box (1), and go up case lid (3) and be located down directly over bottom of the case (4), its characterized in that: a placing component is movably arranged in the box body (1), an air outlet component is arranged right below the placing component, and the air outlet component penetrates through the lower box bottom (4) and is fixedly connected with a circulating component in the lower box bottom; a fresh air component is fixedly arranged in the upper box cover (3), and is fixedly connected with the circulating component;
the placing assembly comprises a left fixing ring (7), a right fixing ring (8), connecting rods (9), hooks (10) and a placing frame (11), the left fixing ring (7) and the right fixing ring (8) are movably connected to two opposite inner walls in the box body (1) respectively, the left fixing ring (7) and the right fixing ring (8) are fixedly connected through four equally-spaced connecting rods (9), two hooks (10) are movably connected to two ends of the circumferential side of each connecting rod (9) respectively, and the two hooks (10) are fixedly connected to two ends of the top of the placing frame (11) respectively;
the fresh air component comprises a filter box (13), an activated carbon layer (14) and filter cotton (15), and the filter box (13) is fixedly arranged in the upper box cover (3); the active carbon layers (14) and the filter cotton (15) are respectively provided with two groups and are distributed in the filter box (13) in a mutually staggered way;
the circulating assembly comprises an air outlet baffle (18) and an air duct (19), the air duct (19) is fixedly mounted at the back of the box body (1), an air outlet is formed in the back of the box body (1), the air outlet is fixedly connected with the air outlet baffle (18) inside, and an included angle of thirty degrees is formed between the air outlet baffle (18) and the air outlet;
the air-out subassembly includes left gear (26), drive gear (27), right gear (28), goes out gas groove (29), first air inlet baffle (30) and second air inlet baffle (31), through two drive gear (27) intermeshing between left gear (26) and right gear (28), left gear (26) one end runs through out gas groove (29) to inside, and with first air inlet baffle (30) fixed connection, right gear (28) run through out gas groove (29) to inside, and with second air inlet baffle (31) fixed connection, first air inlet baffle (30) and second air inlet baffle (31) relative rotation.
2. The laboratory microorganism culture device according to claim 1, wherein the placing assembly further comprises a gear motor (5) and a transmission element (6), the gear motor (5) is fixedly connected inside the upper case cover (3) and is close to the filter box (13), the gear motor (5) penetrates through one side surface of the upper case cover (3) and is fixedly connected with one end of the transmission element (6), and the other end of the transmission element (6) penetrates through the outer side wall of the case body (1) to the inside of the case body and is fixedly connected with the center of the left fixing ring (7).
3. The laboratory microorganism culture device according to claim 1, wherein the fresh air assembly further comprises an exhaust fan (12) and an air supply pipe (16), a mounting hole is formed in the top of the upper box cover (3), the interior of the mounting hole is fixedly connected with an air suction inlet of the exhaust fan (12), the air supply inlet of the exhaust fan (12) is fixedly connected with one end of the filter box (13), the other end of the filter box (13) is fixedly connected with one end of the air supply pipe (16), and the other end of the air supply pipe (16) penetrates through the inner wall of the upper box cover (3) to the interior of the air duct (19).
4. The laboratory microorganism culture device according to claim 1, wherein the circulation assembly further comprises an air suction fan (17), a first air delivery pipe (20), an air pump (21), a second air delivery pipe (22) and an air hopper (23), the air suction fan (17) is fixedly connected inside the air duct (19), and the air suction fan (17) faces the air outlet; one end of the first air delivery pipe (20) penetrates through the inner wall of the lower box bottom (4) to the inner bottom end of the air channel (19), the other end of the first air delivery pipe (20) is fixedly connected with an air suction port of an air pump (21), the air pump (21) is fixedly connected with the inner portion of the lower box bottom (4), an air supply port of the air pump (21) is fixedly connected with one end of the second air delivery pipe (22), the other end of the second air delivery pipe (22) is respectively fixedly connected with four air hoppers (23), and the air hoppers (23) are fixedly connected to the bottom of the air outlet assembly.
5. The laboratory microorganism culture device according to claim 1, wherein there are two sets of the air outlet assemblies, and the two air outlet assemblies are fixedly connected relatively, the air outlet assembly further comprises a stepping motor (24) and a placement box (25), the stepping motor (24) is fixedly connected to the outside of the box body (1), the output end of the stepping motor (24) continuously penetrates through the outer wall of the box body (1) and the placement box (25) is fixedly connected with the left gear (26), the transmission gear (27) and the right gear (28) are sequentially and movably arranged inside the placement box (25), and the placement box (25) is fixedly connected with the air outlet groove (29).
6. The laboratory microorganism culture device according to claim 4, wherein two rectangular through holes are respectively opened in each air outlet groove (29), a first air inlet baffle (30) and a second air inlet baffle (31) are respectively movably arranged in the two rectangular through holes, and an air hopper (23) is respectively fixedly connected to the bottom of each rectangular through hole.
7. The laboratory microorganism culture device according to claim 1, wherein the number of the placing frames (11) is four, every two adjacent placing frames (11) are at a right angle, and the bottom of each placing frame (11) is opposite to the air outlet assembly.
8. A laboratory microbial cultivation device according to claim 1, characterized in that the included angle between the first air intake baffle (30) and the second air intake baffle (31) and the cross section of the air outlet groove (29) is not more than ninety degrees.
9. A laboratory microorganism culture device according to claim 1, characterized in that the material of the filter cotton (15) is glass fiber.
10. A laboratory microorganism culture device according to claim 1, characterized in that a door (2) is hinged outside the housing (1), and the door (2) is opposite to the air outlet.
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