CN113692870A - LED optical prescription for increasing chrysanthemum diameter and improving design and color and application thereof - Google Patents
LED optical prescription for increasing chrysanthemum diameter and improving design and color and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/60—Flowers; Ornamental plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G5/00—Floral handling
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G5/00—Floral handling
- A01G5/06—Devices for preserving flowers
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
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Abstract
The invention utilizes LED combined light to carry out illumination treatment on chrysanthemum varieties with unstable colors, and the effective light quantum flux density of the light formula is 200-‑2·s‑1The chrysanthemum light formula consists of red light, blue light and far-red light, wherein the light quantum flux density percentages of the components are respectively 46% -57% of red light, 30% -40% of blue light and 9% -15% of far-red light, and the LED light formula is beneficial to planting chrysanthemum with stable flower color and better flower shape in high-temperature climate. By applying the optical formula disclosed by the invention, the chrysanthemum with stable color and color under high-temperature climate can be cultivated, the diameter of the chrysanthemum can be effectively increased, and the ornamental value of the chrysanthemum is greatly increased.
Description
Technical Field
The invention relates to the technical field of ornamental horticultural plant cultivation, in particular to an LED optical formula for effectively increasing the diameter of a chrysanthemum capitate inflorescence and improving the color and application thereof.
Background
Chrysanthemum morifolium (Chrysanthemum morifolium) is one of the traditional famous flowers and the most important economic flowers and plants in China, and is widely used in the industries of ornamental flowers, food, tea, fragrant materials and the like. Under the background of global greenhouse effect, and with the progress of urbanization, heat island effect appears around cities, and the climate conditions are not very favorable for the color stability of chrysanthemum. The color pigments of the chrysanthemum petals mainly comprise two major pigments such as anthocyanin (flavonoid) and carotenoid, the color change process of most chrysanthemum is the change process of the anthocyanin pigment, and the anthocyanin is very unstable. Research on chrysanthemum has been focused on breeding new varieties through traditional breeding technology, and researching the medicine/edible value of chrysanthemum, but the research on the aspects of petal color change mechanism, flower color stabilizing cultivation technology and the like is little. Royal phoenix orchid and the like (2011) have studied the color retention technology of chrysanthemum capitula, but are limited to the color retention technology of dried chrysanthemum flowers, and the cultivation technology of how chrysanthemum keeps stable flower color under high-temperature climate conditions is rare.
During the growth and development of plants, the phenotype and color traits of the plants are changed due to the influence of light/light quality, and the influence of the light on the growth and development of the plants is mainly reflected in 3 aspects of the light quality, the light intensity and the light cycle. Oren-Shamir et al found that leaves of plants of the genus Cotinus, after UV blocking, turned green despite their anthocyanin degraded when grown at low temperatures (Oren-Shamir et al) after they had covered the UV blocking plate (Rhus coggygria)and Levi-Nissim, 1997). Rowan et al found that Arabidopsis plants transcriptionally overexpressing PAP1 were under room temperature and high intensity light conditions (22 ℃, 440. mu. mol/m)2S) is dark red, but is transferred to high and low light conditions (30 ℃, 150. mu. mol/m)2/s), plants turn green (Rowan et al, 2009).
The LED is a short name for light-emitting diode, also called light-emitting diode, and has the characteristics of long service life, small heat productivity, easy dispersion and combination control, and the like, and particularly, the LED can distinguish different light qualities, and different light qualities have significantly different influences on plant growth, so that the light-emitting diode (LED) is beginning to be used in the research of plant growth in the middle of the 80 th century. Studies have shown that red light promotes growth of lettuce aerial parts, but monochromatic red light is detrimental to tomato seedling growth (Liuyu soldiers et al, 2020). Supplementing far-red light (732nm) on the basis of red blue (661nm and 447nm) light or supplementing far-red light on the basis of red light obviously increases the fresh weight of the overground part of the lettuce in the seedling stage, the dry and fresh weight of the root system is obviously reduced if the dry and fresh weight of the root system is increased, and the chlorophyll content (SPAD) of the red light and the dry and fresh weight of the root system is obviously reduced after the far-red light is supplemented (Meng et al, 2019). Red and blue light sources 9: 1 can promote the elongation of the inflorescence of the datura flower and increase the number of flowers, and the added blue light can increase the anthocyanin content (Wojciechowska et al, 2019). It can be known that different light colors have different effects on the same plant, and the same light color has different effects on different plants, so finding the most suitable light distribution ratio is also an important cultivation measure for gardening production technology. At present, more researches are carried out on the combination of red and blue monochromatic light and red and blue light to treat horticultural plants, and the research on the optimal proportion of red light, blue light and far-red light for the growth of chrysanthemum is not reported.
In the present invention, "PAR" means photosynthetically active radiation, which means radiation of a specific wavelength that can be utilized for photosynthesis of plants, and the specific wavelength is usually 300nm to 800 nm; "PPFD" refers to Photon Flux Density (Photon Flux Density) and represents the number of photons that irradiate a square meter of leaf within one second, and the wavelength range is 400nm to 700 nm; "PPFD ultraviolet" refers to the violet-ultraviolet radiation band, with a wavelength range of no more than 400 nm; "PPFD blue" refers to the blue-violet radiation band, with a wavelength range of 400nm to 499 nm; "PPFD green" refers to the yellow-green radiation band, with a wavelength range of 500nm to 600 nm; "PPFD red" refers to the red-orange radiation band, with a wavelength range of 601-700 nm; "PPFD far-red" refers to the red-far-red radiation band, with a wavelength range of 701nm to 780 nm; "PPFD Infrared" refers to the red-infrared radiation band, wavelength range > 780 nm; "YPFD" refers to the effective photon flux density, weighted relative quantum efficiency RQE.
Disclosure of Invention
The early research basis of the invention discovers that most chrysanthemum flower colors are very unstable in high-temperature climate. At present, natural light cultivation and fluorescent lamps or LED white light are still used as cultivation modes of light supplementing light sources at night in chrysanthemum production. According to the method, different LED light color treatments are carried out on chrysanthemum varieties with unstable colors, so that the light formula for stabilizing the colors is obtained, the diameter of the chrysanthemum can be effectively increased, the ornamental value of the chrysanthemum is increased, the obtained light formula is favorable for planting the chrysanthemum with stable colors in high-temperature climate, an application foundation is laid for cultivating the chrysanthemum with good heat resistance and stable colors, and meanwhile, the development of a cultivation technology capable of planting the chrysanthemum with stable colors in a large scale is facilitated.
The invention aims to solve the problems and provides an LED light formula for increasing the diameter of chrysanthemum and improving the color.
The invention also provides an application method of the LED light formula.
In order to realize the purpose of the invention, the invention provides an LED light formula for increasing the diameter of chrysanthemum and improving the color, and the formula consists of the following components in percentage by light quantum flux density:
red light: 46 to 57 percent of the total weight of the mixture,
blue light: 30 to 40 percent of the total weight of the mixture,
far-red light: 9 to 15 percent.
Preferably, the LED light formulation has a red light percentage of 48% to 54%.
Preferably, the proportion of blue light in the LED light formulation is 33% to 37%.
Preferably, the far-red light accounts for 11% -13% of the LED light formula.
Preferably, the LED light formula has a red light proportion of 52.2%, a blue light proportion of 35.3% and a far-red light proportion of 12.5%.
Preferably, the LED light formula for increasing the diameter of the chrysanthemum and improving the color and color of the chrysanthemum has the total light quantum flux density of 200-2/s。
The invention also provides an application method of the LED light formula for increasing the diameter and improving the color of the chrysanthemum in chrysanthemum planting.
Preferably, the LED light formula is used in chrysanthemum planting, the illumination environment is 24-26 ℃ in the daytime, and the illumination period is 11.5 h/d.
Preferably, the LED light source is positioned above the plant, and aluminum foil reflective cloth is arranged around the plant to block light.
Preferably, the chrysanthemum is planted in the potting matrix soil after the chrysanthemum is subjected to channel-hole tray seedling culture and the vegetative growth is finished.
Compared with the prior art, the invention has the beneficial effects that:
the chrysanthemum varieties are subjected to illumination treatment by using different LED red light, blue light and far-red light combined light formulas, and the number of head-shaped inflorescences in the initial flowering period is 3 times that of a control group; the ratio of the head-shaped inflorescence in the full-bloom stage to the ratio of the head-shaped inflorescence in the control group is increased by 8-42 percent, and the increase result is obvious; the anthocyanin content of the capitula in the full-bloom stage is increased by 12-31% in comparison with that of a control group, the degradation period is relatively prolonged, the flower color is deepened to different degrees, the flower color is more stable, the ornamental period is prolonged, and the value of the chrysanthemum is greatly increased. Meanwhile, an application foundation is laid for cultivating chrysanthemum with good heat resistance and stable flower color later, and meanwhile, the cultivation technology which can be applied to planting chrysanthemum with stable flower color in a large scale is facilitated to be researched and developed.
Drawings
FIG. 1 is a spectrum diagram of an LED light source; wherein A: LED white light spectrum; b: the LED red, blue and far-red light combines the light source spectrum (RBFR).
Fig. 2 is a diagram of an implementation of a lighting processing arrangement.
FIG. 3 is a graph showing the results of measuring the chlorophyll relative content (SPAD value) and chlorophyll fluorescence parameters (F0, Fv/Fm) of leaves after light treatment.
FIG. 4 is a comparison of potted flowers of Yuyan gradual change orange chrysanthemum in full bloom stage, and CK group is on the left; right RBFR group.
FIG. 5 is a characteristic absorption spectrum of the extract of anthocyanin from the ligulate petals of the delphinium grandis in the full bloom stage of the orange chrysanthemum.
FIG. 6 is a comparison of anthocyanin content in the capitula of the ramuli deltoides at full bloom stage and appearance of the extract.
FIG. 7 is a comparison of the phenotype of the capitate inflorescences of the floury blooming stage of delphinium.
FIG. 8 is a comparison of the CK group and the RBFR group after the irradiation treatment and the 14-day high temperature environment at 28-30 ℃.
Detailed Description
In the present invention, blue light refers to LED light having a wavelength range of 400nm to 499nm, represented by "B"; red light refers to LED light having a wavelength range of 601nm to 700nm, and is denoted by "R"; the far-red light refers to LED light with a wavelength range of 701 nm-780 nm and is represented by 'FR'; white light refers to mixed LED light, denoted by "W". "CK group" refers to the LED white light treated control group; "RBFR group" refers to the test group treated with a combination of red, blue and far-red LED light.
The test methods used in the following examples are all conventional methods unless otherwise specified; the materials, reagents and the like used are, unless otherwise specified, commercially available reagents and materials. The plant illumination analyzer used in the invention has the following model: OHSP-350P, available from Hangzhou rainbow chromatography technologies, Inc.; the LED light source is an ultraviolet/blue/white/red/far-red five-channel lamp panel, and the model is as follows: IGL-AL1150X255-2835-UV/B/W/R/Fr, and the light quantum energy density of LED light beads with various colors can be adjusted.
The invention will be further described with reference to the accompanying drawings and the detailed description below:
example 1
LED light source processing scheme
Set up CK group and RBFR group in this embodiment and realize the light quality combination through five-channel lamp plate LED light bead quantity of regulation and control, the quantity proportion of ruddiness lamp pearl, blue light lamp pearl, far away ruddiness lamp pearl is 7 in this embodiment: 3: 3, because the light conversion efficiency of the light beads of each color is different, the emitted light is not pure color light, contains light of other colors, belongs to a normal phenomenon, the proportion of the light is less than 1 percent, and the average level value of relevant light quality parameters is shown in the table 1 when measured by a plant illumination analyzer within a controllable error range.
In order to avoid the influence of the light sources of other front and rear lamp holders, the two long sides of each lamp holder are shielded by aluminum foil reflective cloth as shown in fig. 2, and the light intensity around the plants can be enhanced to a certain degree by the reflective cloth. The light source is arranged above the plant, and the total amount of PPFD detected near the top of the plant is 240 mu mol/m2The LED spectral distribution is shown in FIG. 1.
TABLE 1 average values of light parameters relevant to light treatment
And (4) analyzing results: in this embodiment, the total photon energy density value PPFD of the final output of the whole five-channel lamp panel is 240.0 μmol/m2As can be seen from the calculations of the data in Table 1 (for convenience of illustration, the percentages remain 1 after the decimal point), the RBFR group had a red fraction of 52.2%, a blue fraction of 35.3%, and a far-red fraction of 12.5%.
2. Chrysanthemum plant cultivation management
In the embodiment, the potted seedlings of the delphinium grandiflorum and the orange chrysanthemum, which have finished vegetative growth, are selected as test materials. Placing the potted seedlings of the delphinium gradually-changed orange chrysanthemum which are ready to enter the reproductive growth stage in the short-day LED light source for treatment, wherein the day/night time length ratio is 11.5h/12.5 h; the ambient temperature was 26 ℃ day and 19 ℃ night. The growth and flowering traits of the relevant plants were measured and recorded periodically during the period. All plants are watered with water soluble fertilizer once in three days, balanced fertilizer is mainly watered before flowering, and Baba ecological compound fertilizer (15-15, total nutrient is more than or equal to 45%, N-P)2O5-K2O, nitrate nitrogen and potassium sulfate) is diluted by 1000 times, and after budding in the later period, high-potassium fertilizer is poured in turn: the Jiashilai Liangda (high-potassium type) macroelement water-soluble fertilizer (10-10-40+ TE, containing nitrate nitrogen) and the balance fertilizer are diluted by 500 times.
3. Determination of the relevant index
The partial trait measurement is referred to the 'test guidance on specificity, consistency and stability of new plant varieties-chrysanthemum' (agricultural industry standard NY/T2228-.
The measurement methods used are mostly: population measurements, individual measurements, population visual observations, and individual visual observations. Tools for group and individual measurements include, but are not limited to, scales, tape measures, and slide calipers. And marking methods for part of special indexes. Selecting 6 single plants which can represent the growth condition of the whole treated plants under different light quality treatment to carry out related index determination.
The relevant determination indexes of the patent test are as follows:
(1) plant overall growth related character index
Plant height: the vertical distance from the above-ground part of the plant to the growing top of the plant; crown width: looking down the plant, wherein the straight line distance between the widest two points of the plant is the overlook distance; internode length: selecting the stem nodes at the middle upper part of the plant for length measurement; the thickness of the trunk: and selecting the position of the upper trunk in the plant to measure the diameter of the main stem. The results of the above measurements are shown in Table 2:
TABLE 2 statistical table of plant overall growth conditions
The above results show that the overall growth of the plants in the CK group and the RBFR group is not obviously different, and the growth of the plants does not change greatly since the plants enter the flowering phase.
(2) Leaf-related trait index
Leaf length: the vertical distance from the bottom to the highest point of the blade except the petiole part; leaf width: the shortest distance from the outermost edge of the left side of the blade to the outermost edge of the right side of the blade; the length of the leaf stalk: the vertical distance of the part without the leaf flesh of the leaf; the number of leaves. The results of the above measurements are shown in Table 3:
TABLE 3 statistical table of leaf-related trait indexes
The above results show that there is no obvious difference in leaf phenotype and leaf number between CK and RBFR plants, but the leaf length of the full-bloom plants in RBFR group is longer and wider than that in CK group.
(3) Relative content of chlorophyll in leaves
The relative chlorophyll content of leaves, i.e., SPAD value, was measured by SPAD-502Plus chlorophyll meter (Konica Minolta, Japan). The results showed that there was no significant difference in the SPAD values of the leaves of the plants in the CK group and the RBFR group, but the relative content of chlorophyll was slightly higher in the RBFR group, as shown in FIG. 3.
(4) Chlorophyll fluorescence parameter of leaves
The fluorescence parameters of chlorophyll in leaves are measured by an OS-30p + chlorophyll fluorescence instrument (OPTI-SCIENCES, USA), the third leaf from the top of a single plant to the bottom is measured, the leaves are put in a completely dark environment for dark adaptation for 10min, and then the values of F0 (initial fluorescence) and Fv/Fm (representing the PSII primary light energy conversion efficiency) are detected. The result shows that the F0 value of the leaves of the chrysanthemum plants in the RBFR group is higher than that of the leaves of the CK group in the bud stage, and the obvious difference exists; at the initial flowering stage, the F0 value of the CK group plant leaves is obviously higher than that of the RBFR group; at full-bloom stage, the F0 value of the leaves of the plants in the RBFR group is greater than that of the CK group, but no significant difference exists, as shown in figure 3.
The maximum photochemical efficiency Fv/Fm can be an important parameter for measuring the degree of photoinhibition. If plants are photoinhibited, Fv/Fm decreases. From the bud stage to the full-bloom stage, there was no obvious difference in Fv/Fm of the leaves of plants in the CK group and RBFR group, as shown in FIG. 3. Overall, both photoplasmically treated Fv/Fm were reduced in the final full-bloom stage compared to the bud stage.
(5) Index of related characters of capitula
The number of buds; maximum bud diameter without color penetration; maximum bud diameter for color penetration; expanding the number of the inflorescences; maximum head-like inflorescence diameter: selecting a flower with the maximum diameter at the top of a single plant for diameter measurement; length and width of the tongue-shaped petals: and selecting one petal of which the outermost periphery of the head-shaped inflorescence can best represent the overall growth condition of the flower for length and width measurement. The results of the above measurements are shown in Table 4:
TABLE 4 statistical table of indexes of related traits of capitula inflorescences
And (4) analyzing results: from the above data, the number of head-like inflorescences developed at the initial flowering stage was 3 times that of the control; the leaves of the chrysanthemum plants in full-bloom stage are increased by 24.8 percent, the width is increased by 14.4 percent, the petioles are increased by 13.6 percent, namely the leaves are larger; the maximum head-shaped inflorescence diameter in the initial flowering period is increased by 14.9 percent compared with that of a control; and the maximum head-shaped inflorescence diameter in the full-bloom stage is increased by 8 percent compared with the contrast.
(6) Taking a picture for recording, and determining the content of anthocyanin in the capitula
And (3) carrying out appearance photographing record on the plants of the delphinium ramosum orange chrysanthemum in the full-bloom stage and the cephalic inflorescences of the plants, and comparing the flower color difference of the cephalic inflorescences of the chrysanthemums processed by the CK group and the RBFR group. The results show that the chrysanthemum flower-head-shaped inflorescence treated by the RBFR group of the combined light of the red, blue and far-red light of the LED is obviously enlarged and the color of the lingulate petals is obviously redder compared with the CK group of the white light of the LED, as shown in figure 4. In addition, to further verify whether the RBFR group treated red pigment content was also higher than that of the control group, the pH differential method was used to determine the anthocyanin content in the petals of the delphinium gradually-changed orange chrysanthemum, according to the methods of Giusti and wrostad (2001), and the specific determination steps were as follows:
preparation of extract and buffer
Extracting solution: 1% hydrochloric acid methanol; buffer solution: 0.4M sodium acetate buffer (pH 4.5) and 0.025M potassium chloride buffer (pH 1.0).
② extraction of petal anthocyanin
0.15g of the lingulate petals are added with liquid nitrogen and ground into powder, 1.5mL of 1% methanol hydrochloride is added, mixed evenly and kept stand on ice for 1 h. After centrifugation at 12000rpm for 20min, approximately 1.2mL of the supernatant was taken in a clean 1.5mL centrifuge tube for use. The color of the pigment extract in the test group was significantly darker than that in the control group as shown in FIG. 6B.
Detecting maximum absorption wavelength
0.05mL of the anthocyanin pigment extract was diluted 5-fold with sodium acetate buffer (pH 4.5) and potassium chloride buffer (pH 1.0), and the mixture was allowed to stand for 30 min. Scanning the above diluent in a full spectrum range with distilled water as blank control, and determining the maximum absorption wavelength of the 'delphin gradually-changed orange' tongue-shaped petal pigment extract to be 520nm according to the maximum absorption peak in a visible light range, as shown in figure 4.
Determination of anthocyanin content
The absorbance was measured at the above-obtained maximum absorption wavelength of 520nm and the wavelength of 700nm as ddH2O is a blank control, and referring to Giusti and wrostald (2001), the anthocyanin content was calculated using the following two-step formula:
A＝(A520-A700)pH1.0-(A520-A700)pH4.5；
anthocyanin content (mg/g) ═ a × MW × DF × 1000 × V)/(∈ × 1 × m)
In the formula, A is absorbance; DF is the fold (5 fold) of sample solution dilution; v is the volume of the extract (0.0012L in this example); m is the sample mass (0.15 g in this example); 1cm is the thickness of the absorption tank; MW is cyanidin-3-glucoside molecular weight (449.2 g/mol); ε represents the molar extinction coefficient of cyanidin-3-glucoside (26900L/(mol cm)).
The results showed that the chrysanthemum capitate anthocyanin content of the RBFR group was 0.3823mg/g FW, which was 23.1% greater than that of the CK group, as shown in FIG. 6, consistent with the appearance change law, as shown in FIG. 4.
Placing the LED in a high-temperature environment for planting after being subjected to light treatment
The treated potted chrysanthemum is placed in a high-temperature environment for 2 weeks at the ambient temperature of 28-30 ℃, as shown in fig. 8, the potted chrysanthemum with the deepened RBFR group color can be placed for a longer time, the color still keeps gorgeous, the potted chrysanthemum is obviously darker than a CK group in the same period, and the ornamental period is effectively prolonged.
Example 2
In the embodiment, powder of delphinium grandiflorum and chrysanthemum are used as test materials, after the chrysanthemum is cultivated by a channel-cave tray method, potted chrysanthemum seedlings entering a reproductive growth stage are prepared, the potted chrysanthemum seedlings are placed in an LED light source with short sunshine for treatment, and the setting is carried outCK group and RBFR group with different proportions, the total light quantum flux density is 200 mu mol/m2(ii)/s, day/night time ratio of 11.5h/12.5 h; the ambient temperature was 25 ℃ day and 18 ℃ night, and other chrysanthemum plant cultivation management conditions and related indicators were determined as in example 1. After chrysanthemum is treated by LED light (RBFR) formulas with different proportions, the maximum head-shaped inflorescence diameter in full-bloom stage is measured, and specific test data are shown in Table 4.
TABLE 4 statistical table of maximum capitulum diameters in full-bloom stage
| Test materials | LED light source | Diameter of inflorescence | Increasing the ratio |
| Radde powder | CK: LED white light | 2.67cm | / |
| Radde powder | R57％，B30％，FR13％ | 3.50cm | 31.09％ |
| Radde powder | R54％，B33％，FR13％ | 3.58cm | 34.08％ |
| Radde powder | R54％，B35％，FR11％ | 3.79cm | 41.95％ |
| Radde powder | R48％，B37％，FR15％ | 3.70cm | 38.58％ |
| Radde powder | R54％，B37％，FR9％ | 3.67cm | 37.45％ |
| Radde powder | R46％，B40％，FR14％ | 3.33cm | 24.72％ |
And (4) analyzing results:
as can be seen from the data in Table 4, the maximum capitate inflorescence diameter in the full-bloom stage is increased by 24.72-41.95% in comparison with that in the CK group through the treatment of each group of LED light formulas, and the increase result is obvious.
Example 3
In the embodiment, the powder chrysanthemum and the orange chrysanthemum of the Yan Yu Yan are used as test materials, the potted chrysanthemum seedlings ready to enter the reproductive growth stage are placed in an LED light source with short sunlight for treatment, a CK group and RBFR groups with different proportions are arranged, and the total luminous flux density is 250 mu mol/m2(ii)/s, day/night time ratio of 11.5h/12.5 h; the ambient temperature was 24 ℃ day and 17 ℃ night, and other chrysanthemum plant cultivation management conditions and related indicators were measured as in example 1. Treating flos Chrysanthemi with LED light (RBFR) formula of different ratiosAnd determining the anthocyanin content in the full-bloom stage, wherein specific test data are shown in a table 5:
TABLE 5 statistic Table of anthocyanin content in full bloom
And (4) analyzing results:
as can be seen from the data in Table 5, the anthocyanin content of the delphinium powder chrysanthemum in the full-bloom stage is increased by 12.1-30.8% in comparison with that of the CK group through the treatment of each group of LED light formulas, and the increase result is obvious; the anthocyanin content of the delphinium gradually-changed orange chrysanthemum in the full-bloom stage is increased by 11.8-22.7% in comparison with that of the CK group, and the increase result is obvious.
The above description is only a preferred embodiment of the present invention, and it will be obvious to those skilled in the art that various other changes and modifications may be made based on the above-described technical solutions and concepts, and all such changes and modifications should fall within the scope of the present invention as claimed.
Claims (10)
1. An LED light formula for increasing the diameter of chrysanthemum and improving the color and luster comprises the following components in percentage by light quantum flux density:
red light: 46 to 57 percent of the total weight of the mixture,
blue light: 30 to 40 percent of the total weight of the mixture,
far-red light: 9 to 15 percent.
2. The LED light formulation of claim 1, wherein the red light percentage is 48% -54%.
3. The LED light formulation of claim 1, wherein the blue light fraction is 33% -37%.
4. The LED light formulation of claim 1, wherein the far-red light fraction is 11% -13%.
5. The LED light formulation of claim 1, wherein the red fraction is 52.2%, the blue fraction is 35.3%, and the far-red fraction is 12.5%.
6. The LED light formula for increasing the diameter and improving the color of chrysanthemum as claimed in any one of claims 1 to 5, wherein the total amount of the light quantum flux density is 200-250 μmol/m2/s。
7. The use of the LED light formula for increasing the diameter and improving the color of chrysanthemum according to claim 6 in chrysanthemum planting.
8. The use of claim 7, wherein the LED light formulation is used in chrysanthemum plantation at an ambient temperature of 24-26 ℃ and a light cycle of 11.5 h-d-1。
9. The use of claim 7, wherein the LED light source is located above the plant, and aluminum foil reflective cloth is arranged around the plant to block light.
10. The use as claimed in claim 7, wherein the chrysanthemum flower is one that has been planted in the potting medium soil after being cultivated by the plug-seedling method and has finished vegetative growth.
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