CN113692804A - Breeding method of high-quality high-yield disease-resistant rice variety - Google Patents
Breeding method of high-quality high-yield disease-resistant rice variety Download PDFInfo
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- CN113692804A CN113692804A CN202111171678.2A CN202111171678A CN113692804A CN 113692804 A CN113692804 A CN 113692804A CN 202111171678 A CN202111171678 A CN 202111171678A CN 113692804 A CN113692804 A CN 113692804A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
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Abstract
The invention discloses a breeding method of a high-quality high-yield disease-resistant rice variety, belonging to the field of rice, and the breeding method of the high-quality high-yield disease-resistant rice variety comprises the following steps: s1: soaking the original rice seeds in the first generation of disease-resistant liquid, and after soaking, carrying out seedling raising and selection to obtain first generation of disease-resistant rice seeds, S2: soaking the first generation of disease-resistant rice seeds in the second generation of disease-resistant liquid, and after soaking, carrying out seedling raising and selection to obtain second generation of disease-resistant rice seeds, S3: the second generation disease-resistant rice seeds are placed in the third generation disease-resistant liquid for soaking, and after soaking, seedling raising and selection are carried out to obtain the third generation disease-resistant rice seeds.
Description
Technical Field
The invention relates to the field of rice, in particular to a breeding method of a high-quality high-yield disease-resistant rice variety.
Background
The rice is a rice cereal crop, the representative seeds are rice, the V is divided into indica rice and japonica rice, early rice and middle and late rice, glutinous rice and non-glutinous rice according to the types of the rice, the V is divided into conventional rice and hybrid rice according to the seed reserving mode, and the V is divided into paddy rice, floating rice and the like according to whether soilless culture is carried out or not; the rice is obtained by removing glume, the rice is called brown rice, the brown rice is ground to remove a rice bran layer, the rice is obtained, nearly half of the world population uses the rice as staple food, the rice can also be used as industrial raw materials for brewing wine and sugar, and the rice hulls and rice straws can be used as livestock feed;
the rice seeds refer to rice seeds, and the yield of the existing rice seeds is easily reduced due to low temperature, viruses and insect pests in the growth process.
Disclosure of Invention
1. Technical problem to be solved
Aiming at the problems in the prior art, the invention aims to provide a breeding method of a high-quality high-yield disease-resistant rice variety, which can realize the improvement of the yield of rice seeds, effectively improve the disease resistance, low temperature resistance and insect resistance of the rice seeds and reduce the yield of the rice seeds reduced by low temperature, viruses and insect pests in the growth process.
2. Technical scheme
In order to solve the above problems, the present invention adopts the following technical solutions.
A breeding method of a high-quality high-yield disease-resistant rice variety comprises the following steps:
s1: soaking the original rice seeds in the first-generation disease-resistant liquid, and then, carrying out seedling raising and selection to obtain first-generation disease-resistant rice seeds;
s2: soaking the first generation of disease-resistant rice seeds in the second generation of disease-resistant liquid, and after soaking, carrying out seedling raising and selection to obtain second generation of disease-resistant rice seeds;
s3: and (3) soaking the second-generation disease-resistant rice seeds in the third-generation disease-resistant liquid, and then carrying out seedling raising and selection to obtain the third-generation disease-resistant rice seeds.
Further, the step S1 includes the following steps:
s101: preparing a first-generation disease-resistant liquid, wherein the first-generation disease-resistant liquid comprises the following components in parts by weight of 0.01-0.05: 1, preparing brassinolide and water according to the proportion, and mixing and stirring the brassinolide and the water to obtain a first-generation disease-resistant liquid;
s102: regulating the temperature of the first generation of disease-resistant liquid, and heating the first generation of disease-resistant liquid by using heating equipment;
s103: soaking, namely placing the original rice seeds in the heated first-generation disease-resistant liquid, and performing centrifugal stirring for 0.2 h; standing for 2h after stirring;
s104: seedling raising, namely uniformly dividing the soaked original rice seeds into a plurality of parts, respectively placing the parts in a plurality of cultivation pots, and growing at the room temperature of 20-25 ℃ until the rice ears are mature;
s105: and (4) selecting, observing the rice ears in a plurality of cultivation pots, and selecting multi-pot rice ears with full fruits and less than 5% of sheath blight incidence as first-generation disease-resistant rice seeds.
Further, the heating temperature of the first generation of disease-resistant liquid is 20-30 ℃.
Further, the step S2 includes the following steps:
s201: preparing a second-generation disease-resistant liquid, wherein the second-generation disease-resistant liquid comprises the following components in parts by weight of 0.1-0.15: 1, preparing brassinolide and water according to the proportion, and mixing and stirring the brassinolide and the water to obtain second-generation disease-resistant liquid;
s202: adjusting the temperature of the second-generation disease-resistant liquid, namely heating the second-generation disease-resistant liquid to 30-40 ℃ by using heating equipment;
s203: soaking, namely placing the first generation of disease-resistant rice seeds in the heated second generation of disease-resistant liquid, and performing centrifugal stirring for 0.2 h; standing for 6h after stirring;
s204: seedling raising, namely uniformly dividing the soaked original rice seeds into a plurality of parts, respectively placing the parts in a plurality of cultivation pots, and growing at the room temperature of 10-20 ℃ until the rice ears are mature;
s205: and (4) selecting, observing the rice ears in a plurality of cultivation pots, and selecting multi-pot rice ears with plump fruits and sheath blight incidence rate lower than 3% from the rice ears, wherein the multi-pot rice ears are second-generation disease-resistant rice seeds.
Further, the step S3 includes the following steps:
s301: preparing a third-generation disease-resistant liquid, wherein the weight parts of the third-generation disease-resistant liquid are 0.1-0.12: 0.5-0.8: 1, preparing brassinolide, an insect-resist agent and water in proportion, and mixing and stirring the brassinolide, the insect-resist agent and the water to obtain a third-generation disease-resistant liquid;
s302: adjusting the temperature of the third-generation disease-resistant liquid, namely heating the third-generation disease-resistant liquid to 30-40 ℃ by using heating equipment;
s303: soaking, placing the second generation disease-resistant rice seeds in the heated third generation disease-resistant liquid, and performing centrifugal stirring for 0.2 h; standing for 3 hours after stirring;
s304: seedling raising, namely uniformly dividing the soaked original rice seeds into a plurality of parts, respectively placing the parts in a plurality of cultivation pots, and growing at the room temperature of 10-20 ℃ until the rice ears are mature;
s305: and (4) selecting, observing the rice ears in a plurality of cultivation pots, and selecting multi-pot rice ears with plump fruits and less than 1% of sheath blight incidence, which are third-generation disease-resistant rice seeds.
Further, the insect-resist agent is one or a combination of more of imidacloprid, omethoate, dimethoate, phoxim, malathion and bentazone.
Furthermore, the rice ears with full fruits are single plants with the rice ear height of more than 100CM and more than 150 grains per ear.
3. Advantageous effects
Compared with the prior art, the invention has the advantages that:
(1) the scheme improves the rice seeds through each step, can improve the yield of the rice seeds, effectively improves the disease resistance, the low temperature resistance and the insect resistance of the rice seeds, and reduces the yield of the rice seeds reduced due to low temperature, viruses and insect pests in the growth process.
Drawings
FIG. 1 is a schematic flow chart of the present invention.
Detailed Description
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention; it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by those skilled in the art without any inventive work are within the scope of the present invention.
Example 1:
referring to fig. 1, a breeding method of a high-quality, high-yield and disease-resistant rice variety includes the following steps:
the method comprises the following steps: soaking the original rice seeds in the first-generation disease-resistant liquid, and then, carrying out seedling raising and selection to obtain first-generation disease-resistant rice seeds;
wherein, the first step comprises the following steps:
A. preparing a first-generation disease-resistant liquid, wherein the first-generation disease-resistant liquid comprises the following components in parts by weight of 0.01-0.05: 1, preparing brassinolide and water according to the proportion, and mixing and stirring the brassinolide and the water to obtain a first-generation disease-resistant liquid;
B. regulating the temperature of the first generation of disease-resistant liquid, namely heating the first generation of disease-resistant liquid to 20-30 ℃ by using heating equipment;
C. soaking, namely placing the original rice seeds in the heated first-generation disease-resistant liquid, and performing centrifugal stirring for 0.2 h; standing for 2h after stirring;
D. seedling raising, namely uniformly dividing the soaked original rice seeds into a plurality of parts, respectively placing the parts in a plurality of cultivation pots, and growing at the room temperature of 20-25 ℃ until the rice ears are mature;
E. selecting, namely observing rice ears in a plurality of cultivation pots, and selecting multi-pot rice ears with plump fruits and sheath blight morbidity lower than 5% from the rice ears, wherein the multi-pot rice ears are first-generation disease-resistant rice seeds;
wherein, the plump rice ears are single plants with the rice ear height of more than 100CM and more than 150 grains per ear;
step two: soaking the first generation of disease-resistant rice seeds in the second generation of disease-resistant liquid, and after soaking, carrying out seedling raising and selection to obtain second generation of disease-resistant rice seeds;
wherein, the second step comprises the following steps:
1. preparing a second-generation disease-resistant liquid, wherein the second-generation disease-resistant liquid comprises the following components in parts by weight of 0.1-0.15: 1, preparing brassinolide and water according to the proportion, and mixing and stirring the brassinolide and the water to obtain second-generation disease-resistant liquid;
2. adjusting the temperature of the second-generation disease-resistant liquid, namely heating the second-generation disease-resistant liquid to 30-40 ℃ by using heating equipment;
3. soaking, namely placing the first generation of disease-resistant rice seeds in the heated second generation of disease-resistant liquid, and performing centrifugal stirring for 0.2 h; standing for 6h after stirring;
4. seedling raising, namely uniformly dividing the soaked original rice seeds into a plurality of parts, respectively placing the parts in a plurality of cultivation pots, and growing at the room temperature of 10-20 ℃ until the rice ears are mature;
5. selecting, namely observing rice ears in a plurality of cultivation pots, and selecting multi-pot rice ears which are full in fruits and have sheath blight morbidity lower than 3% from the rice ears, wherein the multi-pot rice ears are second-generation disease-resistant rice seeds;
step three: soaking the second generation disease-resistant rice seeds in the third generation disease-resistant liquid, and after soaking, carrying out seedling raising and selection to obtain third generation disease-resistant rice seeds;
wherein, the third step comprises the following steps:
preparing a third-generation disease-resistant liquid, wherein the weight parts of the third-generation disease-resistant liquid are as follows: 0.5-0.8: 1, preparing brassinolide, an insect-resist agent and water in proportion, and mixing and stirring the brassinolide, the insect-resist agent and the water to obtain a third-generation disease-resistant liquid;
wherein the insect-resist agent is one or a combination of more of imidacloprid, omethoate, dimethoate, phoxim, malathion and aphicide;
adjusting the temperature of the third-generation disease-resistant liquid, namely heating the temperature of the third-generation disease-resistant liquid to 30-40 ℃ by using heating equipment;
soaking, placing the second-generation disease-resistant rice seeds in the heated third-generation disease-resistant liquid, and performing centrifugal stirring for 0.2 h; standing for 3 hours after stirring;
seedling raising, namely uniformly dividing the soaked original rice seeds into a plurality of parts, respectively placing the parts in a plurality of cultivation pots, and growing at the room temperature of 10-20 ℃ until the rice ears are mature;
and fifthly, selecting, observing the rice ears in a plurality of cultivating pots, and selecting multi-pot rice ears with plump fruits and less than 1% of sheath blight incidence, which are third-generation disease-resistant rice seeds.
In conclusion, the invention improves the rice seeds through various steps, can improve the yield of the rice seeds, effectively improves the disease resistance, the low temperature resistance and the insect resistance of the rice seeds, and reduces the yield reduction caused by low temperature, virus and insect pest in the growth process of the rice seeds
The foregoing is only a preferred embodiment of the present invention; the scope of the invention is not limited thereto. Any person skilled in the art should be able to cover the technical scope of the present invention by equivalent or modified solutions and modifications within the technical scope of the present invention.
Claims (7)
1. A breeding method of a high-quality high-yield disease-resistant rice variety is characterized by comprising the following steps: the method comprises the following steps:
s1: soaking the original rice seeds in the first-generation disease-resistant liquid, and then, carrying out seedling raising and selection to obtain first-generation disease-resistant rice seeds;
s2: soaking the first generation of disease-resistant rice seeds in the second generation of disease-resistant liquid, and after soaking, carrying out seedling raising and selection to obtain second generation of disease-resistant rice seeds;
s3: and (3) soaking the second-generation disease-resistant rice seeds in the third-generation disease-resistant liquid, and then carrying out seedling raising and selection to obtain the third-generation disease-resistant rice seeds.
2. The breeding method of a high-quality high-yield disease-resistant rice variety according to claim 1, characterized in that: the S1 comprises the following steps:
s101: preparing a first-generation disease-resistant liquid, wherein the first-generation disease-resistant liquid comprises the following components in parts by weight of 0.01-0.05: 1, preparing brassinolide and water according to the proportion, and mixing and stirring the brassinolide and the water to obtain a first-generation disease-resistant liquid;
s102: regulating the temperature of the first generation of disease-resistant liquid, and heating the first generation of disease-resistant liquid by using heating equipment;
s103: soaking, namely placing the original rice seeds in the heated first-generation disease-resistant liquid, and performing centrifugal stirring for 0.2 h; standing for 2h after stirring;
s104: seedling raising, namely uniformly dividing the soaked original rice seeds into a plurality of parts, respectively placing the parts in a plurality of cultivation pots, and growing at the room temperature of 20-25 ℃ until the rice ears are mature;
s105: and (4) selecting, observing the rice ears in a plurality of cultivation pots, and selecting multi-pot rice ears with full fruits and less than 5% of sheath blight incidence as first-generation disease-resistant rice seeds.
3. The breeding method of high-quality high-yield disease-resistant rice variety according to claim 2, characterized in that: the heating temperature of the first-generation disease-resistant liquid is 20-30 ℃.
4. The breeding method of a high-quality high-yield disease-resistant rice variety according to claim 1, characterized in that: the S2 comprises the following steps:
s201: preparing a second-generation disease-resistant liquid, wherein the second-generation disease-resistant liquid comprises the following components in parts by weight of 0.1-0.15: 1, preparing brassinolide and water according to the proportion, and mixing and stirring the brassinolide and the water to obtain second-generation disease-resistant liquid;
s202: adjusting the temperature of the second-generation disease-resistant liquid, namely heating the second-generation disease-resistant liquid to 30-40 ℃ by using heating equipment;
s203: soaking, namely placing the first generation of disease-resistant rice seeds in the heated second generation of disease-resistant liquid, and performing centrifugal stirring for 0.2 h; standing for 6h after stirring;
s204: seedling raising, namely uniformly dividing the soaked original rice seeds into a plurality of parts, respectively placing the parts in a plurality of cultivation pots, and growing at the room temperature of 10-20 ℃ until the rice ears are mature;
s205: and (4) selecting, observing the rice ears in a plurality of cultivation pots, and selecting multi-pot rice ears with plump fruits and sheath blight incidence rate lower than 3% from the rice ears, wherein the multi-pot rice ears are second-generation disease-resistant rice seeds.
5. The breeding method of a high-quality high-yield disease-resistant rice variety according to claim 1, characterized in that: the S3 comprises the following steps:
s301: preparing a third-generation disease-resistant liquid, wherein the weight parts of the third-generation disease-resistant liquid are 0.1-0.12: 0.5-0.8: 1, preparing brassinolide, an insect-resist agent and water in proportion, and mixing and stirring the brassinolide, the insect-resist agent and the water to obtain a third-generation disease-resistant liquid;
s302: adjusting the temperature of the third-generation disease-resistant liquid, namely heating the third-generation disease-resistant liquid to 30-40 ℃ by using heating equipment;
s303: soaking, placing the second generation disease-resistant rice seeds in the heated third generation disease-resistant liquid, and performing centrifugal stirring for 0.2 h; standing for 3 hours after stirring;
s304: seedling raising, namely uniformly dividing the soaked original rice seeds into a plurality of parts, respectively placing the parts in a plurality of cultivation pots, and growing at the room temperature of 10-20 ℃ until the rice ears are mature;
s305: and (4) selecting, observing the rice ears in a plurality of cultivation pots, and selecting multi-pot rice ears with plump fruits and less than 1% of sheath blight incidence, which are third-generation disease-resistant rice seeds.
6. A breeding method of high-quality high-yield disease-resistant rice variety as claimed in claim 5, characterized in that: the insect-resist agent is one or a combination of more of imidacloprid, omethoate, dimethoate, phoxim, malathion and sulzer.
7. A breeding method of high-quality high-yield disease-resistant rice variety as claimed in any one of claims 2-6, characterized in that: the rice ears with full fruits are single plants with the rice ear height of more than 100CM and more than 150 grains per ear.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63135303A (en) * | 1986-11-26 | 1988-06-07 | Nissan Chem Ind Ltd | Improvement in maturity of rice plant |
| CN104855269A (en) * | 2015-04-03 | 2015-08-26 | 浙江省嘉兴市农业科学研究院（所） | Method for breeding multiple generations of paddy rice in one year |
| CN105210860A (en) * | 2014-06-18 | 2016-01-06 | 陈祖庆 | The breeding method of a kind of disease resistance paddy rice |
| CN106718855A (en) * | 2016-11-28 | 2017-05-31 | 安徽华安种业有限责任公司 | A kind of selection of rice varieties |
| CN108739158A (en) * | 2018-04-26 | 2018-11-06 | 中国水稻研究所 | Application and application process of the brassinosteroid in alleviating rice florescence High Temperature Stress |
| CN108934857A (en) * | 2018-08-17 | 2018-12-07 | 全州县咸水虹桥米业有限公司 | A kind of breeding method of upland rice seed rice |
-
2021
- 2021-10-08 CN CN202111171678.2A patent/CN113692804A/en not_active Withdrawn
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS63135303A (en) * | 1986-11-26 | 1988-06-07 | Nissan Chem Ind Ltd | Improvement in maturity of rice plant |
| CN105210860A (en) * | 2014-06-18 | 2016-01-06 | 陈祖庆 | The breeding method of a kind of disease resistance paddy rice |
| CN104855269A (en) * | 2015-04-03 | 2015-08-26 | 浙江省嘉兴市农业科学研究院（所） | Method for breeding multiple generations of paddy rice in one year |
| CN106718855A (en) * | 2016-11-28 | 2017-05-31 | 安徽华安种业有限责任公司 | A kind of selection of rice varieties |
| CN108739158A (en) * | 2018-04-26 | 2018-11-06 | 中国水稻研究所 | Application and application process of the brassinosteroid in alleviating rice florescence High Temperature Stress |
| CN108934857A (en) * | 2018-08-17 | 2018-12-07 | 全州县咸水虹桥米业有限公司 | A kind of breeding method of upland rice seed rice |
Non-Patent Citations (1)
| Title |
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| 李元元 等: "油菜素内酯参与调控植物生长发育与抗逆性的机制及其育种应用研究", 《中国农业科技导报》 * |
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