CN113671177A - ICT-based handheld colloidal gold chromatography immunoassay analyzer - Google Patents
ICT-based handheld colloidal gold chromatography immunoassay analyzer Download PDFInfo
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- CN113671177A CN113671177A CN202110938205.4A CN202110938205A CN113671177A CN 113671177 A CN113671177 A CN 113671177A CN 202110938205 A CN202110938205 A CN 202110938205A CN 113671177 A CN113671177 A CN 113671177A
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 title claims abstract description 30
- 238000003018 immunoassay Methods 0.000 title claims abstract description 25
- 238000004587 chromatography analysis Methods 0.000 title claims abstract description 16
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 144
- 238000001514 detection method Methods 0.000 claims abstract description 84
- 238000000034 method Methods 0.000 claims abstract description 31
- 238000005211 surface analysis Methods 0.000 claims abstract description 31
- 230000005540 biological transmission Effects 0.000 claims abstract description 13
- 238000004458 analytical method Methods 0.000 claims description 59
- 238000006243 chemical reaction Methods 0.000 claims description 12
- 238000005286 illumination Methods 0.000 claims description 8
- 230000000694 effects Effects 0.000 claims description 7
- 230000002093 peripheral effect Effects 0.000 claims description 2
- 108091008695 photoreceptors Proteins 0.000 description 8
- 238000003780 insertion Methods 0.000 description 4
- 230000037431 insertion Effects 0.000 description 4
- 238000004364 calculation method Methods 0.000 description 3
- 239000011435 rock Substances 0.000 description 3
- 239000010931 gold Substances 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000011897 real-time detection Methods 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 238000003149 assay kit Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000005034 decoration Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 238000003317 immunochromatography Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
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Abstract
The invention relates to the technical field of immunoassay analyzers, in particular to an ICT-based hand-held colloidal gold chromatography immunoassay analyzer, which comprises an analyzer, a display screen arranged on the upper side of the analyzer, a storage battery arranged on the inner side of the right end of the analyzer, a clamping groove arranged on the right side of the analyzer, a camera arranged on the upper side of the left end of the analyzer and a searchlight arranged on the outer side of the camera, wherein a processor and a wireless transmission module are also arranged on the inner side of the right end of the analyzer, a reagent strip adjusting and fixing system and a detection area surface analysis system are also arranged on the inner side of the analyzer, the detection state of a detection area is analyzed through the arranged detection area surface analysis system, the problem that the reagent strip is not accurately analyzed due to the problems of folding or pollution in the detection process is avoided, the detection area surface analysis system starts to work, and 5 light emitting holes on the transmitting lamp emit light rays, 5 light-emitting holes are respectively irradiated on the edge and the center of a region to be detected on the reagent strip.
Description
Technical Field
The invention relates to the technical field of immunoassay analyzers, in particular to an ICT-based handheld colloidal gold chromatography immunoassay analyzer.
Background
The colloidal gold immunity layer analysis appearance is used for the interpretation of colloidal gold immunochromatography reagent testing result, the analysis appearance utilizes colloidal gold dyestuff mark characteristic, adopt the intelligent instrument of a quantitative analysis who is used for gold mark assay kit of modern photoelectric technology research and development, the analysis appearance can carry out data acquisition to the colloidal gold signal, analysis and calculation, cooperation all kinds of gold mark assay reagents are used, a relevant project parameter for detecting the human body, it supplies the doctor to refer to reacing clinical data, in order to promote the convenience of analysis appearance, the colloidal gold chromatography immunity analyzer of hand-held type begins to appear on the market.
Hand-held type colloidal gold chromatography immunoassay appearance needs the manual inboard of inserting the analysis appearance with the reagent strip, and manual inserting mode can cause sometimes and insert the not in place condition, influence the detection precision of hand-held type analysis appearance to the reagent strip, and simultaneously, the hand-held type analysis appearance still exists and rocks the phenomenon along with the user, make the inboard reagent strip of analysis appearance displacement appear easily, can influence the detection precision of hand-held type analysis appearance to the reagent strip equally, in addition, the inside irradiation detection position of hand-held type analysis appearance is fixed, but the reagent strip is at actual operation's in-process, the preliminary detection region of reagent strip with can appear fold or contaminated phenomenon, still need to carry out the analysis to the situation in preliminary detection region sometimes.
Disclosure of Invention
The invention aims to provide an ICT-based handheld colloidal gold chromatographic immunoassay analyzer to solve the problems in the background art.
In order to achieve the purpose, the invention provides the following technical scheme:
a hand-held colloidal gold chromatography immunoassay analyzer based on ICT comprises an analyzer, a peripheral cloud server, a display screen arranged on the upper side of the analyzer, a storage battery arranged on the inner side of the right end of the analyzer, a clamping groove formed in the right side of the analyzer, a camera arranged on the upper side of the left end of the analyzer and a searchlight arranged on the outer side of the camera, wherein a processor and a wireless transmission module are further arranged on the inner side of the right end of the analyzer;
a detection area surface analysis system for analyzing a detection state of the detection area;
the reagent strip adjusting and fixing system is used for adjusting and fixing the position of the reagent strip;
the entrance reaction mechanism is used for sensing the entrance of the reagent strip;
the driving mechanism is used for adjusting the position of the reagent strip;
the position calibration mechanism is used for enabling the reagent strip to enter an initial position;
and the clamping mechanism is used for fixing the reagent strip before detection.
Furthermore, the detection area surface analysis system comprises an emission lamp and a light receiver, wherein 5 light emitting holes are formed in the emission lamp, connecting lines of the light emitting holes located on the outer side are arranged in a rectangular mode, the light emitting holes located on the inner side are arranged in the central position of the rectangle formed by the connected light emitting holes located on the outer side, the light receiver is symmetrically arranged on the inner side of the analyzer by taking the center line of the camera as the center, light inlet holes are formed in the bottom end of the light receiver, and the light inlet holes are opposite to the light emitting holes one by one.
Further, light receiver's inboard is provided with the photo resistance, the quantity of photo resistance is 5, the photo resistance sets up go into the rear side of unthreaded hole, the inboard of analysis appearance still is provided with current sensor, current sensor and 5 series circuit is constituteed to the photo resistance, detection area surface analysis system still including the analysis pilot lamp, the analysis pilot lamp sets up the upside at the analysis appearance.
Further, actuating mechanism is including setting up the inboard pivot in analysis appearance bottom left side, the outside of pivot is rotated and is connected with first motor, the terminal fixedly connected with rubber circle of main shaft of first motor, through the rotation of rubber circle drives the side-to-side movement and the adjustment of reagent strip.
Furthermore, the position calibration mechanism comprises a laser transmitter and a laser receiver, and the positions of the laser transmitter and the laser receiver correspond up and down.
Further, it includes pinch-off blades and push switch to get into reaction mechanism, the bottom of pinch-off blades rotates with the bottom inboard of analysis appearance to be connected, the bottom fixedly connected with torsional spring of pinch-off blades, the other end of torsional spring and the inboard fixed connection in bottom of analysis appearance, the pinch-off blades is formed by the combination of two different boards of gradient, the bottom right side fixedly connected with depression bar of pinch-off blades, push switch sets up the downside at the depression bar, push switch and the inboard fixed connection in bottom of analysis appearance.
Further, clamping mechanism is including clamp plate and the second motor that can the side-to-side movement, the terminal fixedly connected with lead screw of main shaft of second motor, the outside screwed connection of lead screw has the slide, the slide slides and sets up the inboard at the analysis appearance, the left end fixedly connected with ejector pin of slide, the outside of ejector pin and the inboard sliding connection of analysis appearance, the left end of ejector pin and the right-hand member fixed connection of clamp plate, the left end of clamp plate is the slope form setting.
An ICT-based use method of a handheld colloidal gold chromatography immunoassay analyzer comprises the following steps:
step 1: inserting the reagent strip into the inner side of the analyzer, wherein in the inserting process, the reagent strip adjusting and fixing system starts to work, the reagent strip can press the clamping plate to move downwards, and the pressing switch can be pressed down by the pressing rod in the downward movement process of the clamping plate;
step 2: the driving mechanism starts to work, the first motor rotates, the rotation of the first motor drives the rubber ring to rotate, the reagent strip is driven to adjust the position of the left belt and the right belt through the forward rotation and the reverse rotation of the rubber ring, meanwhile, the position calibration mechanism starts to work, and the laser receiver synchronously receives light rays emitted by the laser emitter;
and step 3: when the driving mechanism drives the reagent strip to move rightwards and blocks light emitted by the laser emitter, the laser receiver cannot receive a light signal, and the reagent strip reaches an initial position;
and 4, step 4: the detection area surface analysis system starts to work, 5 light-emitting holes on the emission lamp emit light, the 5 light-emitting holes irradiate the edge and the center of a to-be-detected area on the reagent strip respectively, the light enters the inner side of the light-entering hole after being reflected by the to-be-detected area and irradiates the photoresistor, in the reflection process, when the reagent strip is wrinkled or polluted, the angle of the reflected light can be changed or become weak, correspondingly, the light entering the light-entering hole can disappear or be weak, the photoresistor can convert the illumination intensity into different resistance signals, the resistors on the 5 photoresistors are overlapped through the series circuit, the current sensor can detect the current on the series circuit in real time, when the current is larger, the smaller the resistor is, the stronger the light received by the photoresistor is, the state of the to-be-detected area is good, and the effect of analyzing the state of the to-be-detected area is achieved, meanwhile, the processor controls the quantity of the lighted analysis indicator lamps according to the magnitude of the current so as to reflect the state of the detection area at the moment;
and 5:
1): when the analysis indicating lamps are all lighted, the detection area state at the moment is better, the clamping mechanism starts to work, the second motor drives the sliding plate to move leftwards, the ejector rod drives the pressing plate to press the clamping plate, so that the reagent strips are clamped and fixed, the searchlight starts to work, the camera starts to take a sample and photograph, and data are transmitted to the cloud server through the wireless transmission module;
2): when the analysis indicating lamps are not all on, the state of the detection area is poor at the moment, the driving mechanism starts to work, the position of the detection area on the reagent strip is adjusted, and after the adjustment, the detection work of the surface analysis system of the detection area is repeated until the area with the good state of the detection area is found.
A method for obtaining results by a hand-held colloidal gold chromatography immunoassay analyzer based on ICT comprises the following steps:
step 1: after the camera takes a picture and samples, the data can be transmitted to a cloud server through a wireless transmission module to be processed and calculated, and meanwhile, the display screen can generate two-dimensional codes according to the information corresponding to the reagent strip;
and 2, scanning the two-dimensional code on the display screen by a user through a mobile phone to obtain a result which corresponds to the reagent strip and is processed and calculated by the cloud server.
Compared with the prior art, the invention has the beneficial effects that:
1. in the invention, the detection area surface analysis system is arranged and used for analyzing the detection state of the detection area, so that the problem that the reagent strip is inaccurate in analysis due to the problem of corrugation or pollution is avoided in the detection process, the detection area surface analysis system starts to work, 5 light emitting holes on the emission lamp emit light, the 5 light emitting holes respectively irradiate the edge and the center of a region to be detected on the reagent strip, the light enters the inner side of the light entering hole after being reflected by the region to be detected and irradiates the photoresistor, in the reflection process, when the reagent strip is corrugated or polluted, the angle of the reflected light is changed or weakened, correspondingly, the light entering hole disappears or is weakened, the photoresistor converts the illumination intensity into different resistance signals, and the resistances on the 5 photoresistors are superposed by the series circuit, the current sensor that sets up can real-time detection series circuit on the electric current, when the electric current is big more, resistance this moment is less, and the light that the photo resistance received is stronger, waits to detect regional state good to play the analysis and wait the effect of detecting regional state, simultaneously, the treater can come the quantity of control analysis pilot lamp according to the size of electric current and light and react the detection area state this moment.
2. According to the invention, the reagent strip position is adjusted and fixed through the reagent strip adjusting and fixing system, so that the problem of improper insertion caused by manual reagent strip insertion is avoided, the problem of reagent strip deviation caused by shaking of an analyzer is also avoided, the accuracy of a detection structure is further increased, the reagent strip enters the reaction mechanism for sensing the entering of the reagent strip, the driving mechanism is used for adjusting the position of the reagent strip, the position calibration mechanism is used for enabling the reagent strip to enter the initial position, and the clamping mechanism is used for fixing the reagent strip before detection.
Drawings
In order to more clearly illustrate the technical solution of the present invention, the drawings needed to be used in the technical description of the present invention will be briefly introduced below, and it is apparent that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without inventive labor.
FIG. 1 is a schematic view of the overall appearance mounting structure of the present invention;
FIG. 2 is a schematic view of the overall internal mounting structure of the present invention;
FIG. 3 is a schematic view of the appearance of the mounting structure of the hold-down plate of the present invention;
FIG. 4 is a schematic view of the internal mounting structure of the optical receiver of the present invention;
FIG. 5 is a schematic view of the outer structure of the clamping plate according to the present invention;
FIG. 6 is a side view of the mounting structure of the light receiver of the present invention;
FIG. 7 is a schematic view of the mounting structure shown at A in FIG. 2 according to the present invention;
FIG. 8 is a schematic flow chart of a system for obtaining an analysis result of the present invention.
In the figure: 1. an analyzer; 2. a clamping groove; 3. a storage battery; 4. a display screen; 5. an analysis indicator light; 6. a reagent strip; 7. an emission lamp; 7a, a light outlet; 8. a camera; 9. a current sensor; 10. a processor; 11. a position calibration mechanism; 11a, a laser transmitter; 11b, a laser receiver; 12. a drive mechanism; 12a, a rubber ring; 12b, a first motor; 12c, a rotating shaft; 13. a clamping plate; 14. a torsion spring; 15. a push switch; 16. a pressure lever; 17. a second motor; 18. a screw rod; 19. a slide plate; 20. a top rod; 21. a compression plate; 22. a light receiver; 22a, a light inlet hole; 22b, a photoresistor; 23. a wireless transmission module; 24. a searchlight.
Detailed Description
The present invention will be further described with reference to the following detailed description, wherein the drawings are for illustrative purposes only and are not intended to be limiting, wherein certain elements may be omitted, enlarged or reduced in size, and are not intended to represent the actual dimensions of the product, so as to better illustrate the detailed description of the invention.
Example 1
Referring to fig. 1, fig. 2, fig. 3, fig. 5 and fig. 7, the present invention provides a technical solution:
a hand-held colloidal gold chromatography immunoassay analyzer based on ICT comprises an analyzer (1), a display screen (4) arranged on the upper side of the analyzer (1), a storage battery (3) arranged on the inner side of the right end of the analyzer (1), a clamping groove (2) formed in the right side of the analyzer (1), a camera (8) arranged on the upper side of the left end of the analyzer (1) and a searchlight (24) arranged on the outer side of the camera (8), a processor (10) and a wireless transmission module (23) are further arranged on the inner side of the right end of the analyzer (1), a reagent strip adjusting and fixing system is further arranged on the inner side of the analyzer (1), and the reagent strip adjusting and fixing system comprises a driving mechanism (12), a position calibration mechanism (11), a clamping mechanism and an entering reaction mechanism;
the reagent strip adjusting and fixing system is used for adjusting and fixing the position of the reagent strip (6);
the reagent strip feeding and fixing mechanism is used for sensing the feeding of a reagent strip (6), the reagent strip (6) is inserted into the inner side of the analyzer (1), in the inserting process, the reagent strip adjusting and fixing system starts to work, the reagent strip (6) can press the clamping plate (13) to move downwards, and in the downward movement process of the clamping plate (13), the pressing switch (15) can be pressed down through the pressing rod (16);
the driving mechanism (12) is used for adjusting the position of the reagent strip (6), the driving mechanism (12) works, the first motor (12 b) rotates, the rotation of the first motor (12 b) drives the rubber ring (12 a) to rotate, and the forward and backward rotation of the rubber ring (12 a) drives the reagent strip (6) to adjust the position of the left and right belts;
the position calibration mechanism (11) is used for enabling the reagent strip (6) to enter an initial position, the position calibration mechanism (11) starts to work, the laser receiver (11 b) synchronously receives light rays emitted by the laser emitter (11 a), when the driving mechanism (12) drives the reagent strip (6) to move rightwards and blocks the light rays emitted by the laser emitter (11 a), the laser receiver (11 b) cannot receive light ray signals, and the reagent strip (6) reaches the initial position;
the clamping mechanism is used for fixing the reagent strip (6) before detection, the clamping mechanism works, the second motor (17) drives the sliding plate (19) to move leftwards, and the ejector rod (20) drives the pressing plate (21) to prop against the clamping plate (13), so that the reagent strip (6) is clamped and fixed;
specifically, the driving mechanism comprises a rotating shaft (12 c) arranged on the inner side of the left side of the bottom end of the analyzer (1), the outer side of the rotating shaft (12 c) is rotatably connected with a first motor (12 b), the tail end of a main shaft of the first motor (12 b) is fixedly connected with a rubber ring (12 a), the rubber ring (12 a) rotates to drive the reagent strip (6) to move left and right and adjust, and the rubber ring (12 a) is used for increasing friction force;
specifically, the position calibration mechanism (11) comprises a laser transmitter (11 a) and a laser receiver (11 b), and the positions of the laser transmitter (11 a) and the laser receiver (11 b) are vertically corresponding;
specifically, the entry reaction mechanism comprises a clamping plate (13) and a press switch (15), the bottom end of the clamping plate (13) is rotatably connected with the inner side of the bottom end of the analyzer (1), a torsion spring (14) is fixedly connected with the bottom end of the clamping plate (13), the other end of the torsion spring (14) is fixedly connected with the inner side of the bottom end of the analyzer (1), the clamping plate (13) is formed by combining two plates with different gradients, a pressure lever (16) is fixedly connected to the right side of the bottom end of the clamping plate (13), the press switch (15) is arranged on the lower side of the pressure lever (16), the press switch (15) is fixedly connected with the inner side of the bottom end of the analyzer (1), and after the press switch (15) is pressed down, the driving mechanism (12) starts to work;
specifically, the clamping mechanism comprises a pressing plate (21) and a second motor (17) which can move left and right, the tail end of a main shaft of the second motor (17) is fixedly connected with a screw rod (18), the outer side of the screw rod (18) is spirally connected with a sliding plate (19), the sliding plate (19) is arranged on the inner side of the analyzer (1) in a sliding manner, the left end of the sliding plate (19) is fixedly connected with a mandril (20), the outer side of the mandril (20) is slidably connected with the inner side of the analyzer (1), the left end of the mandril (20) is fixedly connected with the right end of the pressing plate (21), the left end of the pressing plate (21) is arranged in an inclined manner, the second motor (17) rotates to drive the screw rod (18), the rotation of the screw rod (18) can drive the sliding plate (19) to move left or right, when the sliding plate (19) moves left, the pressing plate (13) can be pressed through the mandril (20) and the pressing plate (21), thereby realizing the fixation of the reagent strip (6).
By adopting the technical scheme: through reagent strip adjustment fixing system, accomplish adjustment and fixed to the reagent strip position, avoid the manual work to insert the not in place problem of inserting that reagent strip (6) brought, also avoid the analyzer to rock the reagent strip skew problem of bringing simultaneously, further increase the accuracy nature to the detection structure, reagent strip adjustment fixing system, a position for to reagent strip (6) is adjusted and is fixed, after reagent strip (6) manual insertion certain distance, get into reaction mechanism, an entering for response reagent strip (6), actuating mechanism (12), a position for to reagent strip (6) is adjusted, position calibration mechanism (11), a reagent strip (6) enters into initial position, clamping mechanism, a reagent strip (6) for before the detection is fixed.
Example 2
Referring to fig. 1, fig. 2, fig. 4 and fig. 6, the present invention provides a technical solution:
an ICT-based handheld colloidal gold chromatography immunoassay analyzer comprises an analyzer (1), a display screen (4) arranged on the upper side of the analyzer (1), a storage battery (3) arranged on the inner side of the right end of the analyzer (1), a clamping groove (2) formed in the right side of the analyzer (1), a camera (8) arranged on the upper side of the left end of the analyzer (1) and a searchlight (24) arranged on the outer side of the camera (8), a processor (10) and a wireless transmission module (23) are further arranged on the inner side of the right end of the analyzer (1), and a detection area surface analysis system is further arranged on the inner side of the analyzer (1);
specifically, the detection area surface analysis system comprises an emission lamp (7) and a photoreceptor (22), wherein 5 light-emitting holes (7 a) are formed in the emission lamp (7), the connecting lines of the light-emitting holes (7 a) positioned on the outer side are arranged in a rectangular shape, the light-emitting holes (7 a) positioned on the inner side are arranged at the central position of the rectangular connecting lines of the light-emitting holes (7 a) positioned on the outer side, the photoreceptor (22) is symmetrically arranged on the inner side of the analyzer (1) by taking the center line of a camera (8) as the center, the bottom end of the photoreceptor (22) is provided with light-entering holes (22 a), the positions of the light-entering holes (22 a) and the positions of the light-emitting holes (7 a) are opposite to each other, the inner sides of the photoreceptor (22) are provided with photosensitive resistors (22 b), the number of the photosensitive resistors (22 b) is 5, the photosensitive resistors (22 b) are arranged on the rear side of the light-entering holes (22 a), and the inner side of the analyzer (1) is further provided with a current sensor (9), the current sensor (9) and 5 photoresistors (22 b) form a series circuit, the detection area surface analysis system further comprises an analysis indicator lamp (5), the analysis indicator lamp (5) is arranged on the upper side of the analyzer (1), the detection area surface analysis system starts to work, 5 light-emitting holes (7 a) in the emission lamp (7) emit light, the 5 light-emitting holes (7 a) respectively irradiate the edge and the center of a region to be detected on the reagent strip (6), the light enters the inner side of the light-emitting hole (22 a) after being reflected by the region to be detected and irradiates the photoresistors (22 b), in the reflection process, when the reagent strip (6) is wrinkled or polluted, the angle of the reflected light can be changed or weakened, correspondingly, the light entering the light-emitting hole (22 a) can disappear or be weakened, the photoresistors (22 b) can convert the illumination intensity into different resistance signals, the series circuit enables the resistors on the 5 photoresistors (22 b) to be superposed, the arranged current sensor (9) can detect the current on the series circuit in real time, when the current is larger, the smaller the resistor is, the stronger the light received by the photoresistors (22 b) is, and the state of the area to be detected is good, so that the effect of analyzing the state of the area to be detected is achieved, and meanwhile, the processor (10) can control the quantity of the lighted analysis indicating lamps (5) according to the magnitude of the current to reflect the state of the area to be detected;
by adopting the technical scheme: through the arranged detection area surface analysis system, the detection state of the detection area is analyzed, the problem that the analysis of a reagent strip (6) is inaccurate due to the problem of corrugation or pollution is avoided in the detection process, the detection area surface analysis system starts to work, 5 light emitting holes (7 a) on an emission lamp (7) emit light, the 5 light emitting holes (7 a) respectively irradiate the edge and the center of a region to be detected on the reagent strip (6), the light enters the inner side of a light inlet hole (22 a) after being reflected by the region to be detected and irradiates a photoresistor (22 b), in the reflection process, when the reagent strip (6) is corrugated or polluted, the angle of the reflected light changes or becomes weak, correspondingly, the light entering the light inlet hole (22 a) disappears or is weak, and the photoresistor (22 b) converts the illumination intensity into different resistance signals, the series circuit makes the resistance on 5 photo resistance (22 b) superpose mutually, and the current on the current sensor (9) that sets up can real-time detection series circuit, and when the electric current is big more, the resistance that this moment is little, and the light that photo resistance (22 b) received is stronger, and it is good to detect regional state to play the analysis and detect the effect of regional state, and simultaneously, treater (10) can control the quantity that analysis pilot lamp (5) are lighted according to the size of electric current and react the detection region state this moment.
Example 3
Referring to fig. 1-8, the present invention provides a technical solution:
a hand-held colloidal gold chromatography immunoassay analyzer based on ICT comprises an analyzer (1), a display screen (4) arranged on the upper side of the analyzer (1), a storage battery (3) arranged on the inner side of the right end of the analyzer (1), a clamping groove (2) formed in the right side of the analyzer (1), a camera (8) arranged on the upper side of the left end of the analyzer (1) and a searchlight (24) arranged on the outer side of the camera (8), a processor (10) and a wireless transmission module (23) are further arranged on the inner side of the right end of the analyzer (1), a reagent strip adjusting and fixing system and a detection area surface analysis system are further arranged on the inner side of the analyzer (1), and the reagent strip adjusting and fixing system comprises a driving mechanism (12), a position calibration mechanism (11), a clamping mechanism and an entering reaction mechanism;
a detection area surface analysis system for analyzing a detection state of the detection area;
the reagent strip adjusting and fixing system is used for adjusting and fixing the position of the reagent strip (6);
the reagent strip feeding and fixing mechanism is used for sensing the feeding of a reagent strip (6), the reagent strip (6) is inserted into the inner side of the analyzer (1), in the inserting process, the reagent strip adjusting and fixing system starts to work, the reagent strip (6) can press the clamping plate (13) to move downwards, and in the downward movement process of the clamping plate (13), the pressing switch (15) can be pressed down through the pressing rod (16);
the driving mechanism (12) is used for adjusting the position of the reagent strip (6), the driving mechanism (12) works, the first motor (12 b) rotates, the rotation of the first motor (12 b) drives the rubber ring (12 a) to rotate, and the forward and backward rotation of the rubber ring (12 a) drives the reagent strip (6) to adjust the position of the left and right belts;
the position calibration mechanism (11) is used for enabling the reagent strip (6) to enter an initial position, the position calibration mechanism (11) starts to work, the laser receiver (11 b) synchronously receives light rays emitted by the laser emitter (11 a), when the driving mechanism (12) drives the reagent strip (6) to move rightwards and blocks the light rays emitted by the laser emitter (11 a), the laser receiver (11 b) cannot receive light ray signals, and the reagent strip (6) reaches the initial position;
the clamping mechanism is used for fixing the reagent strip (6) before detection, the clamping mechanism works, the second motor (17) drives the sliding plate (19) to move leftwards, and the ejector rod (20) drives the pressing plate (21) to prop against the clamping plate (13), so that the reagent strip (6) is clamped and fixed;
specifically, the detection area surface analysis system comprises an emission lamp (7) and a photoreceptor (22), wherein 5 light-emitting holes (7 a) are formed in the emission lamp (7), the connecting lines of the light-emitting holes (7 a) positioned on the outer side are arranged in a rectangular shape, the light-emitting holes (7 a) positioned on the inner side are arranged at the central position of the rectangular connecting lines of the light-emitting holes (7 a) positioned on the outer side, the photoreceptor (22) is symmetrically arranged on the inner side of the analyzer (1) by taking the center line of a camera (8) as the center, the bottom end of the photoreceptor (22) is provided with light-entering holes (22 a), the positions of the light-entering holes (22 a) and the positions of the light-emitting holes (7 a) are opposite to each other, the inner sides of the photoreceptor (22) are provided with photosensitive resistors (22 b), the number of the photosensitive resistors (22 b) is 5, the photosensitive resistors (22 b) are arranged on the rear side of the light-entering holes (22 a), and the inner side of the analyzer (1) is further provided with a current sensor (9), the current sensor (9) and 5 photoresistors (22 b) form a series circuit, the detection area surface analysis system further comprises an analysis indicator lamp (5), the analysis indicator lamp (5) is arranged on the upper side of the analyzer (1), the detection area surface analysis system starts to work, 5 light-emitting holes (7 a) in the emission lamp (7) emit light, the 5 light-emitting holes (7 a) respectively irradiate the edge and the center of a region to be detected on the reagent strip (6), the light enters the inner side of the light-emitting hole (22 a) after being reflected by the region to be detected and irradiates the photoresistors (22 b), in the reflection process, when the reagent strip (6) is wrinkled or polluted, the angle of the reflected light can be changed or weakened, correspondingly, the light entering the light-emitting hole (22 a) can disappear or be weakened, the photoresistors (22 b) can convert the illumination intensity into different resistance signals, the series circuit enables the resistors on the 5 photoresistors (22 b) to be superposed, the arranged current sensor (9) can detect the current on the series circuit in real time, when the current is larger, the smaller the resistor is, the stronger the light received by the photoresistors (22 b) is, and the state of the area to be detected is good, so that the effect of analyzing the state of the area to be detected is achieved, and meanwhile, the processor (10) can control the quantity of the lighted analysis indicating lamps (5) according to the magnitude of the current to reflect the state of the area to be detected;
specifically, the driving mechanism comprises a rotating shaft (12 c) arranged on the inner side of the left side of the bottom end of the analyzer (1), the outer side of the rotating shaft (12 c) is rotatably connected with a first motor (12 b), the tail end of a main shaft of the first motor (12 b) is fixedly connected with a rubber ring (12 a), the rubber ring (12 a) rotates to drive the reagent strip (6) to move left and right and adjust, and the rubber ring (12 a) is used for increasing friction force;
specifically, the position calibration mechanism (11) comprises a laser transmitter (11 a) and a laser receiver (11 b), and the positions of the laser transmitter (11 a) and the laser receiver (11 b) are vertically corresponding;
specifically, the entry reaction mechanism comprises a clamping plate (13) and a press switch (15), the bottom end of the clamping plate (13) is rotatably connected with the inner side of the bottom end of the analyzer (1), a torsion spring (14) is fixedly connected with the bottom end of the clamping plate (13), the other end of the torsion spring (14) is fixedly connected with the inner side of the bottom end of the analyzer (1), the clamping plate (13) is formed by combining two plates with different gradients, a pressure lever (16) is fixedly connected to the right side of the bottom end of the clamping plate (13), the press switch (15) is arranged on the lower side of the pressure lever (16), the press switch (15) is fixedly connected with the inner side of the bottom end of the analyzer (1), and after the press switch (15) is pressed down, the driving mechanism (12) starts to work;
specifically, the clamping mechanism comprises a pressing plate (21) and a second motor (17) which can move left and right, the tail end of a main shaft of the second motor (17) is fixedly connected with a screw rod (18), the outer side of the screw rod (18) is spirally connected with a sliding plate (19), the sliding plate (19) is arranged on the inner side of the analyzer (1) in a sliding manner, the left end of the sliding plate (19) is fixedly connected with a mandril (20), the outer side of the mandril (20) is slidably connected with the inner side of the analyzer (1), the left end of the mandril (20) is fixedly connected with the right end of the pressing plate (21), the left end of the pressing plate (21) is arranged in an inclined manner, the second motor (17) rotates to drive the screw rod (18), the rotation of the screw rod (18) can drive the sliding plate (19) to move left or right, when the sliding plate (19) moves left, the pressing plate (13) can be pressed through the mandril (20) and the pressing plate (21), thereby realizing the fixation of the reagent strip (6).
By adopting the technical scheme: through the arranged detection area surface analysis system, the detection state of the detection area is analyzed, the problem that the analysis of a reagent strip (6) is inaccurate due to the problem of corrugation or pollution is avoided in the detection process, the detection area surface analysis system starts to work, 5 light emitting holes (7 a) on an emission lamp (7) emit light, the 5 light emitting holes (7 a) respectively irradiate the edge and the center of a region to be detected on the reagent strip (6), the light enters the inner side of a light inlet hole (22 a) after being reflected by the region to be detected and irradiates a photoresistor (22 b), in the reflection process, when the reagent strip (6) is corrugated or polluted, the angle of the reflected light changes or becomes weak, correspondingly, the light entering the light inlet hole (22 a) disappears or is weak, and the photoresistor (22 b) converts the illumination intensity into different resistance signals, the series circuit enables the resistors on the 5 photoresistors (22 b) to be superposed, the arranged current sensor (9) can detect the current on the series circuit in real time, when the current is larger, the smaller the resistor is, the stronger the light received by the photoresistors (22 b) is, and the state of the area to be detected is good, so that the effect of analyzing the state of the area to be detected is achieved, and meanwhile, the processor (10) can control the quantity of the lighted analysis indicating lamps (5) according to the magnitude of the current to reflect the state of the area to be detected; through reagent strip adjustment fixing system, accomplish adjustment and fixed to the reagent strip position, avoid the manual work to insert the not in place problem of inserting that reagent strip (6) brought, also avoid the analyzer to rock the reagent strip skew problem of bringing simultaneously, further increase the accuracy nature to the detection structure, reagent strip adjustment fixing system, a position for to reagent strip (6) is adjusted and is fixed, after reagent strip (6) manual insertion certain distance, get into reaction mechanism, an entering for response reagent strip (6), actuating mechanism (12), a position for to reagent strip (6) is adjusted, position calibration mechanism (11), a reagent strip (6) enters into initial position, clamping mechanism, a reagent strip (6) for before the detection is fixed.
The invention relates to an ICT-based handheld colloidal gold chromatography immunoassay analyzer, which comprises the following working methods:
the reagent strip (6) is inserted into the inner side of the analyzer (1), in the inserting process, a reagent strip adjusting and fixing system starts to work, the reagent strip (6) can press a clamping plate (13) to move downwards, a press switch (15) can be pressed down by a press rod (16) in the downward movement process of the clamping plate (13), a driving mechanism (12) starts to work, a first motor (12 b) rotates, the rotation of the first motor (12 b) drives a rubber ring (12 a) to rotate, the reagent strip (6) is driven to carry out position adjustment of left and right belts through positive and negative rotation of the rubber ring (12 a), meanwhile, a position calibrating mechanism (11) starts to work, a laser receiver (11 b) synchronously receives light emitted by a laser emitter (11 a), and when the driving mechanism (12) drives the reagent strip (6) to move rightwards and blocks the light emitted by the laser emitter (11 a), the laser receiver (11 b) cannot receive light signals, the reagent strip (6) reaches an initial position, the detection area surface analysis system starts to work, 5 light-emitting holes (7 a) on the emission lamp (7) emit light, the 5 light-emitting holes (7 a) respectively irradiate the edge and the center of a region to be detected on the reagent strip (6), the light enters the inner side of the light-entering hole (22 a) after being reflected by the region to be detected and irradiates the photoresistor (22 b), in the reflection process, when the reagent strip (6) is wrinkled or polluted, the angle of the reflected light can be changed or changed weakly, correspondingly, the light entering the light-entering hole (22 a) can disappear or be weak, the photoresistor (22 b) can convert the illumination intensity into different resistance signals, and the series circuits enable the resistances on the 5 photoresistors (22 b) to be superposed, the current sensor (9) can detect the current on the series circuit in real time, when the current is larger, the resistance at the moment is smaller, the light received by the photoresistor (22 b) is stronger, the state of a region to be detected is good, so that the state of the region to be detected is analyzed, meanwhile, the processor (10) can control the lighting quantity of the analysis indicating lamp (5) according to the current to reflect the state of the detection region at the moment, when the analysis indicating lamp (5) is completely lighted, the state of the detection region at the moment is better, the clamping mechanism starts to work, the second motor (17) drives the sliding plate (19) to move leftwards, the ejector rod (20) drives the pressing plate (21) to push the clamping plate (13), so that the reagent strip (6) is clamped and fixed, the searchlight (24) starts to work, the camera (8) starts to take a sample and photograph, and data are transmitted to the cloud server through the wireless transmission module, when the analysis indicator lamps (5) are not all on, the state of the detection area is poor at the moment, the driving mechanism (12) starts to work, the position of the detection area on the reagent strip (6) is adjusted, and after the adjustment, the detection work of the detection area surface analysis system is repeated until the area with the good state of the detection area is found.
The invention relates to an ICT-based handheld colloidal gold chromatography immunoassay analyzer, which comprises the following steps:
after camera (8) took a picture and take a sample, data can be passed through wireless transmission module (23) and transmitted to the high in the clouds server and handle the calculation, and simultaneously, display screen (4) can be with the information generation two-dimensional code that this reagent strip (6) corresponds, and the user of service obtains the result that is handled the calculation by the high in the clouds server that this reagent strip (6) correspond through the two-dimensional code on cell-phone scanning display screen (4).
The principles and embodiments of the present invention are explained herein using specific examples, which are presented only to assist in understanding the method and its core concepts of the present invention. The foregoing is only a preferred embodiment of the present invention, and it should be noted that there are objectively infinite specific structures due to the limited character expressions, and it will be apparent to those skilled in the art that a plurality of modifications, decorations or changes may be made without departing from the principle of the present invention, and the technical features described above may be combined in a suitable manner; such modifications, variations, combinations, or adaptations of the invention using its spirit and scope, as defined by the claims, may be directed to other uses and embodiments.
Claims (9)
1. The utility model provides a hand-held type colloidal gold chromatography immunoassay appearance based on ICT, includes the display screen that the high in the clouds server of analysis appearance, peripheral hardware, analysis appearance upside set up, the battery that the analysis appearance right-hand member inboard set up and the centre gripping groove of seting up on analysis appearance right side, the camera that the analysis appearance left end upper side set up and the searchlight that the camera outside set up, its characterized in that: the inner side of the right end of the analyzer is also provided with a processor and a wireless transmission module, the inner side of the analyzer is also provided with a reagent strip adjusting and fixing system and a detection area surface analysis system, and the reagent strip adjusting and fixing system comprises a driving mechanism, a position calibration mechanism, a clamping mechanism and an entry reaction mechanism;
a detection area surface analysis system for analyzing a detection state of the detection area;
the reagent strip adjusting and fixing system is used for adjusting and fixing the position of the reagent strip;
the entrance reaction mechanism is used for sensing the entrance of the reagent strip;
the driving mechanism is used for adjusting the position of the reagent strip;
the position calibration mechanism is used for enabling the reagent strip to enter an initial position;
and the clamping mechanism is used for fixing the reagent strip before detection.
2. The ICT-based handheld colloidal gold chromatographic immunoassay analyzer of claim 1, characterized in that: the detection area surface analysis system comprises a transmitting lamp and a light receiver, wherein 5 light emitting holes are formed in the transmitting lamp, connecting lines of the light emitting holes located on the outer side are arranged in a rectangular mode, the light emitting holes located on the inner side are arranged in the central position of the rectangle formed by the connected light emitting holes located on the outer side, the light receiver is symmetrically arranged on the inner side of the analyzer by taking the center line of a camera as the center, the light emitting holes are formed in the bottom end of the light receiver, and the light emitting holes are opposite to the light emitting holes one by one.
3. The ICT-based handheld colloidal gold chromatographic immunoassay analyzer of claim 2, characterized in that: the inboard of light receiver is provided with photo resistance, photo resistance's quantity is 5, photo resistance sets up the rear side of income unthreaded hole, the inboard of analysis appearance still is provided with current sensor, current sensor and 5 series circuit is constituteed to photo resistance, detection area surface analysis system still including the analysis pilot lamp, the analysis pilot lamp sets up the upside at the analysis appearance.
4. An ICT-based hand-held colloidal gold chromatographic immunoassay analyzer in accordance with any one of claims 1-3, wherein: actuating mechanism is including setting up the inboard pivot in analysis appearance bottom left side, the outside of pivot is rotated and is connected with first motor, the terminal fixedly connected with rubber circle of main shaft of first motor, through the rotation of rubber circle drives the side-to-side movement and the adjustment of reagent strip.
5. An ICT-based hand-held colloidal gold chromatographic immunoassay analyzer in accordance with any one of claims 1-3, wherein: the position calibration mechanism comprises a laser transmitter and a laser receiver, and the positions of the laser transmitter and the laser receiver correspond up and down.
6. An ICT-based hand-held colloidal gold chromatographic immunoassay analyzer in accordance with any one of claims 1-3, wherein: the entering reaction mechanism comprises a clamping plate and a press switch, the bottom end of the clamping plate is rotatably connected with the inner side of the bottom end of the analyzer, the bottom end of the clamping plate is fixedly connected with a torsion spring, the other end of the torsion spring is fixedly connected with the inner side of the bottom end of the analyzer, the clamping plate is formed by combining two plates with different gradients, the bottom right side of the clamping plate is fixedly connected with a pressing rod, the press switch is arranged on the lower side of the pressing rod, and the press switch is fixedly connected with the inner side of the bottom end of the analyzer.
7. The ICT-based handheld colloidal gold chromatographic immunoassay analyzer of claim 6, which is characterized in that: clamping mechanism includes clamp plate and the second motor that can the side-to-side movement, the terminal fixedly connected with lead screw of main shaft of second motor, the outside screwed connection of lead screw has the slide, the slide slides and sets up the inboard at the analysis appearance, the left end fixedly connected with ejector pin of slide, the outside of ejector pin and the inboard sliding connection of analysis appearance, the left end of ejector pin and the right-hand member fixed connection of clamp plate, the left end of clamp plate is the slope form setting.
8. A method of using an ICT-based hand-held colloidal gold chromatography immunoassay analyzer in accordance with claim 1, the method comprising:
step 1: inserting the reagent strip into the inner side of the analyzer, wherein in the inserting process, the reagent strip adjusting and fixing system starts to work, the reagent strip can press the clamping plate to move downwards, and the pressing switch can be pressed down by the pressing rod in the downward movement process of the clamping plate;
step 2: the driving mechanism starts to work, the first motor rotates, the rotation of the first motor drives the rubber ring to rotate, the reagent strip is driven to adjust the position of the left belt and the right belt through the forward rotation and the reverse rotation of the rubber ring, meanwhile, the position calibration mechanism starts to work, and the laser receiver synchronously receives light rays emitted by the laser emitter;
and step 3: when the driving mechanism drives the reagent strip to move rightwards and blocks light emitted by the laser emitter, the laser receiver cannot receive a light signal, and the reagent strip reaches an initial position;
and 4, step 4: the detection area surface analysis system starts to work, 5 light-emitting holes on the emission lamp emit light, the 5 light-emitting holes irradiate the edge and the center of a to-be-detected area on the reagent strip respectively, the light enters the inner side of the light-entering hole after being reflected by the to-be-detected area and irradiates the photoresistor, in the reflection process, when the reagent strip is wrinkled or polluted, the angle of the reflected light can be changed or become weak, correspondingly, the light entering the light-entering hole can disappear or be weak, the photoresistor can convert the illumination intensity into different resistance signals, the resistors on the 5 photoresistors are overlapped through the series circuit, the current sensor can detect the current on the series circuit in real time, when the current is larger, the smaller the resistor is, the stronger the light received by the photoresistor is, the state of the to-be-detected area is good, and the effect of analyzing the state of the to-be-detected area is achieved, meanwhile, the processor controls the quantity of the lighted analysis indicator lamps according to the magnitude of the current so as to reflect the state of the detection area at the moment;
and 5:
1): when the analysis indicating lamps are all lighted, the detection area state at the moment is better, the clamping mechanism starts to work, the second motor drives the sliding plate to move leftwards, the ejector rod drives the pressing plate to press the clamping plate, so that the reagent strips are clamped and fixed, the motor searchlight starts to work, the camera starts to take a sample and photograph, and data are transmitted to the cloud server through the wireless transmission module;
2): when the analysis indicating lamps are not all on, the state of the detection area is poor at the moment, the driving mechanism starts to work, the position of the detection area on the reagent strip is adjusted, and after the adjustment, the detection work of the surface analysis system of the detection area is repeated until the area with the good state of the detection area is found.
9. A method for obtaining results of an ICT-based handheld colloidal gold chromatographic immunoassay analyzer in accordance with claim 1, wherein the method comprises:
step 1: after the camera takes a picture and samples, the data can be transmitted to a cloud server through a wireless transmission module to be processed and calculated, and meanwhile, the display screen can generate two-dimensional codes according to the information corresponding to the reagent strip;
and 2, scanning the two-dimensional code on the display screen by a user through a mobile phone to obtain a result which corresponds to the reagent strip and is processed and calculated by the cloud server.
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