CN113667602A - High-purity stem cell culture automation equipment - Google Patents
High-purity stem cell culture automation equipment Download PDFInfo
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- CN113667602A CN113667602A CN202110886737.8A CN202110886737A CN113667602A CN 113667602 A CN113667602 A CN 113667602A CN 202110886737 A CN202110886737 A CN 202110886737A CN 113667602 A CN113667602 A CN 113667602A
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- 210000000130 stem cell Anatomy 0.000 title claims abstract description 56
- 238000004113 cell culture Methods 0.000 title claims abstract description 27
- 239000007788 liquid Substances 0.000 claims abstract description 42
- 239000000725 suspension Substances 0.000 claims abstract description 24
- 238000010257 thawing Methods 0.000 claims abstract description 15
- 238000005070 sampling Methods 0.000 claims abstract description 4
- 238000012360 testing method Methods 0.000 claims description 34
- 239000002184 metal Substances 0.000 claims description 29
- 238000005057 refrigeration Methods 0.000 claims description 25
- 238000009795 derivation Methods 0.000 claims description 16
- 238000005338 heat storage Methods 0.000 claims description 13
- 238000005485 electric heating Methods 0.000 claims description 10
- 239000004065 semiconductor Substances 0.000 claims description 8
- 238000001125 extrusion Methods 0.000 claims description 7
- 238000007789 sealing Methods 0.000 claims description 6
- 238000003466 welding Methods 0.000 claims description 5
- 239000011148 porous material Substances 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 2
- 238000000034 method Methods 0.000 abstract description 7
- 238000013461 design Methods 0.000 abstract description 3
- 238000011081 inoculation Methods 0.000 abstract description 2
- 238000013404 process transfer Methods 0.000 abstract description 2
- 239000006143 cell culture medium Substances 0.000 description 4
- 238000009825 accumulation Methods 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 230000001174 ascending effect Effects 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 108010082117 matrigel Proteins 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
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Abstract
The invention discloses high-purity stem cell culture automation equipment, and relates to the technical field of novel stem cell culture devices. The automatic dynamic-guiding liquid taking structure is arranged on one side of the constant-temperature cultivation base and used for quantitatively sampling stem cell cultivation and quantitatively injecting culture liquid. According to the invention, through the integral structural design of the device, the device is convenient for completing the automatic and efficient implementation of the integral process of stem cell culture, avoids the matching of a plurality of different devices, reduces the time consumed by process transfer in the culture process, and improves the efficiency in the thawing process, the forming efficiency of heavy suspension and the continuous forming efficiency of inoculation and culture.
Description
Technical Field
The invention relates to the technical field of novel stem cell culture devices, in particular to high-purity stem cell culture automation equipment.
Background
Stem cells are cells with unlimited or immortal self-renewal capacity, can produce at least one type of highly differentiated progeny cells, and corresponding culture equipment is needed for culturing and researching stem cells.
Disclosure of Invention
The invention aims to provide high-purity stem cell culture automation equipment, which aims to solve the existing problems: the existing stem cell culture equipment is often required to be matched with a plurality of different pieces of equipment for use in the using process, so that a large amount of turnover time is required during stem cell culture, and the culture efficiency of the equipment used in the stem cell culture process is low.
In order to achieve the purpose, the invention provides the following technical scheme: the high-purity stem cell culture automation equipment comprises a constant-temperature culture base, wherein the constant-temperature culture base is used for forming constant-temperature culture;
the constant-temperature sealing cover is positioned at the top end of the constant-temperature cultivation base and is used for being matched with the constant-temperature cultivation base to form constant-temperature cultivation;
the automatic dynamic guiding liquid taking structure is arranged on one side of the constant-temperature cultivation base and is used for quantitatively sampling stem cell cultivation and quantitatively injecting a culture solution;
the automatic unfreezing and centrifuging structure is arranged on one side inside the constant-temperature cultivation base and is used for unfreezing, reducing and centrifugally mixing stem cells;
the automatic heavy suspension structure is arranged inside the constant-temperature cultivation base, is positioned on one side of the automatic unfreezing centrifugal structure, and is used for stably suspending stem cells;
the automatic culture structure, the automatic culture structure sets up in the inside that the base was cultivated to the constant temperature, the automatic culture structure is located one side that automatic heavy suspension structure kept away from automatic thawing centrifugal structure, the automatic culture structure is used for the stable drive cultivation to stem cell.
Preferably, the constant-temperature cultivation base comprises a base body, a placing support plate, a heat storage delivery pipe, a resistance wire, a first temperature-conducting metal rod, a refrigeration delivery box, a semiconductor refrigeration plate and a second temperature-conducting metal rod, the top end of the inner part of the base main body is fixedly connected with a placing and carrying plate, the inner part of the base main body is fixedly provided with a first temperature-conducting metal rod and a second temperature-conducting metal rod, the first temperature conducting metal rod and the second temperature conducting metal rod are both positioned at the bottom end of the carrying plate, the first temperature conducting metal rod is positioned at the two ends of the heat storage delivery pipe, the heat storage eduction tube is fixed at one end of the base main body, a plurality of electric heating resistance wires are fixed at the inner side of the heat storage eduction tube, the refrigeration is derived the case and is fixed in the other end of base main part, the both ends of refrigeration are derived the case and are all led temperature metal pole fixed connection with the second, the top fixedly connected with semiconductor refrigeration board of case is derived in the refrigeration.
Preferably, the automatic thawing centrifugal structure comprises a first movable guide output block, a first motor, a driving output gear, a first driven gear, a second driven gear, a thawing test tube, a centrifugal test tube, a temperature-conducting lantern ring and an electric hot plate, wherein the bottom of the inner side of the first movable guide output block is fixedly connected with the first motor through a screw, the output end of the first motor is fixedly connected with the driving output gear, one side of the top end of the first movable guide output block is rotatably connected with the first driven gear, the other side of the top end of the first movable guide output block is rotatably connected with the second driven gear, the first driven gear and the second driven gear are both meshed with the driving output gear, the top end of the first driven gear is fixedly connected with the thawing test tube, the top end of the second driven gear is fixedly connected with the centrifugal test tube, and the outer side of the thawing test tube is fixedly connected with the temperature-conducting lantern ring, one end of the temperature-conducting lantern ring is fixedly connected with an electric heating plate through a temperature-conducting glue.
Preferably, automatic heavy suspension structure includes first reciprocal automatic output structure and heavy suspension test tube, the one end fixedly connected with of first reciprocal automatic output structure hangs down the heavy suspension test tube.
Preferably, the automatic culture structure comprises a second reciprocating automatic output structure and a six-hole plate, and the top end of the second reciprocating automatic output structure is fixedly connected with the six-hole plate.
Preferably, the structure of first reciprocal automatic output structure is the same with the structure of the reciprocal automatic output structure of second, first reciprocal automatic output structure and the reciprocal automatic output structure of second all include built-in power piece, reserve spout, spacing backup pad, second motor, eccentric power board, reciprocal guide block and the pole of deriving, the spout has been seted up to the one end of built-in power piece, the inside welding of built-in power piece has spacing backup pad, screw fixedly connected with second motor is passed through to the one end of spacing backup pad, the output fixedly connected with eccentric power board of second motor, the bottom of eccentric power board is rotated and is connected with reciprocal guide block, the one end that eccentric power board was kept away from to reciprocal guide block is rotated and is connected with the guide pole that pushes away.
Preferably, first reciprocal automatic output structure and the reciprocal automatic output structure of second still include spacing carrying block, linkage derivation piece, guide polished rod, cooperation ejector pad and elastic support pole, the spacing carrying block of top fixedly connected with of built-in power piece, the top sliding connection of spacing carrying block derives the piece in the linkage, the both sides that the piece was derived in the linkage all are connected with spacing carrying block through elastic support pole, elastic support pole is used for spacing linkage derivation piece, avoids linkage derivation piece overtravel, the inboard welding of linkage derivation piece has the guide polished rod, the outside sliding connection of guide polished rod has the cooperation ejector pad, the top of ejector rod and the cooperation ejector pad welded connection.
Preferably, the automatic movable guiding liquid taking structure comprises a trackless cylinder, a cylinder slider, a second movable guiding output block, a third motor, a movable guiding gear shaft, a guiding limiting guide block, a following rack column and a hydraulic piston cylinder, wherein the cylinder slider is connected to the inner side of the trackless cylinder in a sliding manner, the second movable guiding output block is welded to one end of the top end of the cylinder slider, the guiding limiting guide block is fixedly connected to one end of the second movable guiding output block, the third motor is fixedly connected to one side of the second movable guiding output block through a screw, the movable guiding gear shaft is fixedly connected to the output end of the third motor, the following rack column is connected to the inner side of the guiding limiting guide block in a sliding manner, one end of the movable guiding gear shaft is meshed with the following rack column, and the hydraulic piston cylinder is fixedly connected to one end of the top end of the following rack column through a screw.
Preferably, the automatic movable guiding liquid taking structure further comprises a liquid taking column, a guide column, a fourth motor, a screw rod, a linkage push rod, an extrusion plate and an electromagnetic valve pipe, wherein the liquid taking column is fixedly connected with the output end of the hydraulic piston cylinder, the guide column is welded at the top end of the liquid taking column, the fourth motor is fixedly connected with the top end of the guide column through the screw, the screw rod is fixedly connected with the output end of the fourth motor, driving grooves are formed in two sides of the guide column, the outer side of the screw rod is connected with the linkage push rod through threads, the linkage push rod is connected with the driving grooves in a sliding mode, the extrusion plate is welded at the bottom end of the linkage push rod, the linkage push rod is connected with the liquid taking column in a sliding mode, and the electromagnetic valve pipe is fixedly connected with the bottom end of the liquid taking column.
Compared with the prior art, the invention has the beneficial effects that:
according to the invention, through the integral structural design of the device, the device is convenient for completing the automatic and efficient implementation of the integral process of stem cell culture, avoids the matching of a plurality of different devices, reduces the time consumed by process transfer in the culture process, and improves the efficiency in the thawing process, the forming efficiency of heavy suspension and the continuous forming efficiency of inoculation and culture.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural view of the present invention as a whole;
FIG. 2 is an exploded view of the present invention as a whole;
FIG. 3 is a partial structural view of the constant temperature cultivation base according to the present invention;
FIG. 4 is a schematic view of a partial structure of an automated thawing centrifugation structure according to the present invention;
FIG. 5 is a schematic diagram of a partial structure of the automated resuspension structure of the present invention;
FIG. 6 is a schematic partial structure view of an automated culture structure according to the present invention;
FIG. 7 is a partial schematic view of the reciprocating automatic output structure of the present invention;
FIG. 8 is a side view of the reciprocating automatic output structure of the present invention;
fig. 9 is a partial structural schematic view of the automatic dynamic guiding liquid taking structure of the present invention.
In the figure: 1. a constant temperature cultivation base; 2. sealing the cover at constant temperature; 3. an automatic dynamic guiding liquid taking structure; 4. automatically thawing the centrifuge structure; 5. an automated resuspension structure; 6. an automated culture structure; 7. a base body; 8. placing a carrying plate; 9. a heat storage delivery pipe; 10. an electric heating resistance wire; 11. a first temperature-conducting metal rod; 12. a refrigeration lead-out box; 13. a semiconductor refrigeration plate; 14. a second temperature-conducting metal rod; 15. a first dynamic conductance output block; 16. a first motor; 17. a drive output gear; 18. a first driven gear; 19. a second driven gear; 20. unfreezing the test tube; 21. centrifuging the test tube; 22. a temperature-conducting lantern ring; 23. an electric hot plate; 24. a first reciprocating automatic output structure; 25. resuspending the tube; 26. a second reciprocating automatic output structure; 27. six orifice plates; 28. a power block is arranged in the power box; 29. reserving a chute; 30. a limiting support plate; 31. a second motor; 32. an eccentric power plate; 33. a reciprocating push guide block; 34. pushing the guide rod; 35. a limit carrying block; 36. a linkage leading-out block; 37. a guide polish rod; 38. a push block is matched; 39. an elastic support bar; 40. a trackless cylinder; 41. a cylinder slider; 42. a second dynamic conductance output block; 43. a third motor; 44. a movable guide gear shaft; 45. a guide limit guide block; 46. following rack columns; 47. a hydraulic piston cylinder; 48. taking a liquid column; 49. a guide post; 50. a fourth motor; 51. a screw; 52. a linkage push rod; 53. a pressing plate; 54. a solenoid valve tube.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Please refer to fig. 1-2:
a high-purity stem cell culture automation device comprises a constant-temperature culture base 1, wherein the constant-temperature culture base 1 is used for forming constant-temperature culture;
the constant-temperature sealing cover 2 is positioned at the top end of the constant-temperature cultivation base 1, and the constant-temperature sealing cover 2 is used for being matched with the constant-temperature cultivation base 1 to form constant-temperature cultivation;
the automatic dynamic guiding liquid taking structure 3 is arranged on one side of the constant-temperature cultivation base 1, and the automatic dynamic guiding liquid taking structure 3 is used for quantitatively sampling stem cell cultivation and quantitatively injecting culture liquid;
the automatic unfreezing centrifugal structure 4 is arranged on one side inside the constant-temperature cultivation base 1, and the automatic unfreezing centrifugal structure 4 is used for unfreezing, reducing and centrifugally mixing stem cells;
the automatic heavy suspension structure 5 is arranged inside the constant-temperature cultivation base 1, the automatic heavy suspension structure 5 is located on one side of the automatic thawing centrifugal structure 4, and the automatic heavy suspension structure 5 is used for stably resuspending stem cells;
automatic cultivate structure 6, automatic cultivate structure 6 and set up in the inside that base 1 was cultivated to constant temperature, automatic cultivate structure 6 and be located automatic heavy suspension structure 5 and keep away from one side of automatic centrifugal structure 4 that unfreezes, automatic cultivate structure 6 is used for the stable drive cultivation to stem cell.
Please refer to fig. 3:
the constant temperature cultivating base 1 comprises a base main body 7, a placing support plate 8, a heat storage eduction tube 9, an electric heating resistance wire 10, a first temperature conducting metal rod 11, a refrigeration eduction box 12, a semiconductor refrigeration plate 13 and a second temperature conducting metal rod 14, a placing support plate 8 is fixedly connected to the top end inside the base main body 7, a first temperature conducting metal rod 11 and a second temperature conducting metal rod 14 are fixed inside the base main body 7, the first temperature conducting metal rod 11 and the second temperature conducting metal rod 14 are both located at the bottom end of the placing support plate 8, the first temperature conducting metal rod 11 is located at both ends of a heat storage delivery pipe 9, the heat storage delivery pipe 9 is fixed at one end of the base main body 7, a plurality of electric heating resistance wires 10 are fixed on the inner side of the heat storage delivery pipe 9, a refrigeration delivery box 12 is fixed at the other end of the base main body 7, both ends of the refrigeration delivery box 12 are fixedly connected with the second temperature conducting metal rod 14, and a semiconductor refrigeration plate 13 is fixedly connected to the top end of the refrigeration delivery box 12;
the temperature in the constant-temperature cultivation base 1 needs to be maintained at thirty-seven ℃, when the temperature is higher than thirty-seven ℃, the refrigeration in the refrigeration leading-out box 12 is completed by controlling the semiconductor refrigeration plate 13, the connection between the refrigeration leading-out box 12 and the second temperature conducting metal rod 14 is utilized, the cooling temperature at the refrigeration leading-out box 12 is led out by utilizing the second temperature conducting metal rod 14, so that the temperature in the constant-temperature cultivation base 1 is reduced until thirty-seven ℃, the temperature is stopped, when the temperature in the constant-temperature cultivation base 1 is lower than thirty-seven ℃, the accumulated heat quantity in the heat accumulation leading-out pipe 9 is started by the electrothermal filament 10, the heat accumulated in the heat accumulation leading-out pipe 9 is led out to the inside of the base main body 7 by utilizing the first temperature conducting metal rod 11, and the heat accumulated in the heat accumulation leading-out pipe 9 is stopped until thirty-seven ℃;
please refer to fig. 4:
the automatic thawing centrifugal structure 4 comprises a first movable guide output block 15, a first motor 16, a driving output gear 17, a first driven gear 18, a second driven gear 19, a thawing test tube 20, a centrifugal test tube 21, a temperature guide lantern ring 22 and an electric hot plate 23, wherein the bottom end of the inner side of the first movable guide output block 15 is fixedly connected with the first motor 16 through a screw, the output end of the first motor 16 is fixedly connected with the driving output gear 17, one side of the top end of the first movable guide output block 15 is rotatably connected with the first driven gear 18, the other side of the top end of the first movable guide output block 15 is rotatably connected with the second driven gear 19, the first driven gear 18 and the second driven gear 19 are both meshed with the driving output gear 17, the top end of the first driven gear 18 is fixedly connected with the thawing test tube 20, the top end of the second driven gear 19 is fixedly connected with the centrifugal test tube 21, the outer side of the thawing test tube 20 is fixedly connected with the temperature guide lantern ring 22, one end of the temperature-conducting lantern ring 22 is fixedly connected with an electric heating plate 23 through temperature-conducting glue;
the temperature of the temperature-conducting lantern ring 22 is raised through the electric heating plate 23 and output, the temperature-raising temperature of the electric heating plate 23 is led out to the unfreezing test tube 20 through the temperature-raising lantern ring 22, the temperature inside the unfreezing test tube 20 is raised, stem cells are pumped and discharged from liquid nitrogen to the inside of the unfreezing test tube 20 through the automatic movable guiding liquid-taking structure 3, the stem cells are unfrozen inside the unfreezing test tube 20, the driving of the driving output gear 17 is completed through the first motor 16 during unfreezing, the torque output of the unfreezing test tube 20 is completed through the driving of the driving output gear 17 through the meshing of the driving output gear 17 and the first driven gear 18, the unfreezing test tube 20 rotates to drive the stem cells to be unfrozen rapidly, the unfrozen stem cells are extracted to the inside the centrifugal test tube 21 through the automatic movable guiding liquid-taking structure 3, a stem cell culture medium is injected inside the centrifugal test tube 21, by utilizing the meshing of the second driven gear 19 and the driving output gear 17, the second driven gear 19 transmits the torque to the centrifugal test tube 21, so that the stem cells are fully fused with the stem cell culture medium, and the preparation before the culture is finished;
please refer to fig. 5:
the automatic heavy suspension structure 5 comprises a first reciprocating automatic output structure 24 and a heavy suspension test tube 25, wherein one end of the first reciprocating automatic output structure 24 is fixedly connected with the heavy suspension test tube 25;
injecting the centrifuged stem cell culture medium into the heavy suspension test tube 25 under the driving of the automatic dynamic guiding liquid taking structure 3, and driving the heavy suspension test tube 25 to shake by using the first reciprocating automatic output structure 24 so that the centrifuged stem cell culture medium blows, sucks and uniformly mixes stem cell sediments;
please refer to fig. 6:
the automatic culture structure 6 comprises a second reciprocating automatic output structure 26 and six pore plates 27, wherein the six pore plates 27 are fixedly connected to the top end of the second reciprocating automatic output structure 26;
discarding the balanced ready-to-use matrigel, adding the uniformly blown stem cell suspension into the six-hole plate 27 through the automatic dynamic guiding liquid taking structure 3, supplementing culture liquid in the six-hole plate 27, and uniformly distributing cells by utilizing the driving of the second reciprocating automatic output structure 26;
please refer to fig. 7-8:
the structure of the first reciprocating automatic output structure 24 is the same as that of the second reciprocating automatic output structure 26, the first reciprocating automatic output structure 24 and the second reciprocating automatic output structure 26 both comprise an internal power block 28, a reserved chute 29, a limiting support plate 30, a second motor 31, an eccentric power plate 32, a reciprocating push guide block 33 and a push rod 34, the reserved chute 29 is formed in one end of the internal power block 28, the limiting support plate 30 is welded inside the internal power block 28, one end of the limiting support plate 30 is fixedly connected with the second motor 31 through a screw, the output end of the second motor 31 is fixedly connected with the eccentric power plate 32, the bottom end of the eccentric power plate 32 is rotatably connected with the reciprocating push guide block 33, and one end, far away from the eccentric power plate 32, of the reciprocating push guide block 33 is rotatably connected with the push rod 34;
the first reciprocating automatic output structure 24 and the second reciprocating automatic output structure 26 further comprise a limiting carrying block 35, a linkage guiding block 36, a guiding polished rod 37, a matching pushing block 38 and an elastic supporting rod 39, the top end of the built-in power block 28 is fixedly connected with the limiting carrying block 35, the top end of the limiting carrying block 35 is slidably connected with the linkage guiding block 36, two sides of the linkage guiding block 36 are connected with the limiting carrying block 35 through the elastic supporting rod 39, the elastic supporting rod 39 is used for limiting the linkage guiding block 36 to avoid the linkage guiding block 36 from exceeding the range, the guiding polished rod 37 is welded on the inner side of the linkage guiding block 36, the matching pushing block 38 is slidably connected on the outer side of the guiding polished rod 37, and the top end of the guiding rod 34 is welded with the matching pushing block 38;
the torque output of the eccentric power plate 32 is completed by the second motor 31, so that the eccentric power plate 32 rotates, the eccentric design of the eccentric power plate 32 is utilized, the change derivation power from the farthest end to the nearest end exists in the rotation process of the eccentric power plate 32, the change derivation power is transmitted to the derivation rod 34 by the reciprocating derivation block 33, the derivation rod 34 is utilized to drive the matching derivation block 38 to slide at the guide polished rod 37, and the weight change generated in the sliding process of the matching derivation block 38 is utilized to drive the matching derivation block 36 to slide at the limit carrying block 35, so that the reciprocating translation drive is formed;
please refer to fig. 9:
the automatic movable guide liquid taking structure 3 comprises a trackless air cylinder 40, an air cylinder slide block 41, a second movable guide output block 42, a third motor 43, a movable guide gear shaft 44, a guide limiting guide block 45, a following rack column 46 and a hydraulic piston cylinder 47, wherein the inner side of the trackless air cylinder 40 is connected with the air cylinder slide block 41 in a sliding manner, one end of the top end of the air cylinder slide block 41 is welded with the second movable guide output block 42, one end of the second movable guide output block 42 is fixedly connected with the guide limiting guide block 45, one side of the second movable guide output block 42 is fixedly connected with the third motor 43 through a screw, the output end of the third motor 43 is fixedly connected with the movable guide gear shaft 44, the inner side of the guide limiting guide block 45 is connected with the following rack column 46 in a sliding manner, one end of the movable guide gear shaft 44 is meshed with the following rack column 46, and one end of the top end of the following rack column 46 is fixedly connected with the hydraulic piston cylinder 47 through a screw;
automatic change and move guide and get liquid structure 3 and still include and get liquid post 48, guide post 49, fourth motor 50, screw rod 51, linkage push rod 52, stripper plate 53 and solenoid valve pipe 54, liquid post 48 is got to the output fixedly connected with of hydraulic piston cylinder 47, the top welding of getting liquid post 48 has guide post 49, screw fixedly connected with fourth motor 50 is passed through on guide post 49's top, fourth motor 50's output fixedly connected with screw rod 51, the drive groove is all seted up to guide post 49's both sides, screw rod 51's the outside is passed through the screw and is connected with linkage push rod 52, linkage push rod 52 and drive groove sliding connection, the bottom welding of linkage push rod 52 has stripper plate 53, linkage push rod 52 and the sliding connection of getting liquid post 48, the bottom fixedly connected with solenoid valve pipe 54 of getting liquid post 48.
When the stem cells need to be transversely displaced, the trackless air cylinder 40 is used for guiding the air cylinder slide block 41 to form transverse driving, when the height of injected stem cells is adjusted, the third motor 43 is controlled to complete torque driving of the movable guide gear shaft 44, the movable guide gear shaft 44 is meshed with the following rack column 46, the following rack column 46 is stirred by the movable guide gear shaft 44 to complete lifting adjustment, the hydraulic piston cylinder 47 is used for extending or contracting the output end to drive and adjust the length positioning adjustment of the injected position of the stem cells, the fourth motor 50 is controlled to rotate forwardly, the fourth motor 50 is used for driving the screw rod 51 to complete rotation, the screw rod 51 is connected with the linkage push rod 52 through threads, the linkage push rod 52 obtains torque, the linkage push rod 52 is connected with the driving groove in a sliding manner, the torque at the linkage push rod 52 is limited to form sliding displacement, the extrusion plate 53 is driven to complete ascending by utilizing the sliding displacement of the linkage push rod 52, an ascending air column is formed inside the liquid taking column 48 by utilizing the sliding of the extrusion plate 53 in the liquid taking column 48, the stem cells are pumped into the liquid taking column 48 through the electromagnetic valve tube 54, the electromagnetic valve tube 54 is controlled to be closed after being pumped, when the stem cells are output, the extrusion plate 53 is enabled to be pressed down in the liquid taking column 48 by controlling the reverse output torque of the fourth motor 50, the electromagnetic valve tube 54 is opened, the stem cells are enabled to be discharged out of the liquid taking column 48, and the injection of the stem cells is completed.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (9)
1. The utility model provides a high-purity stem cell culture automation equipment which characterized in that: the constant-temperature cultivation device comprises a constant-temperature cultivation base (1), wherein the constant-temperature cultivation base (1) is used for forming constant-temperature cultivation;
the constant-temperature sealing cover (2) is positioned at the top end of the constant-temperature cultivation base (1), and the constant-temperature sealing cover (2) is used for being matched with the constant-temperature cultivation base (1) to form constant-temperature cultivation;
the automatic dynamic guiding liquid taking structure (3) is arranged on one side of the constant-temperature cultivation base (1), and the automatic dynamic guiding liquid taking structure (3) is used for quantitatively sampling stem cell cultivation and quantitatively injecting culture liquid;
the automatic unfreezing and centrifuging structure (4) is arranged on one side inside the constant-temperature cultivation base (1), and the automatic unfreezing and centrifuging structure (4) is used for unfreezing, reducing and centrifugally mixing stem cells;
the automatic heavy suspension structure (5), the automatic heavy suspension structure (5) is arranged inside the constant-temperature cultivation base (1), the automatic heavy suspension structure (5) is positioned on one side of the automatic thawing centrifugal structure (4), and the automatic heavy suspension structure (5) is used for stably resuspending stem cells;
automatic cultivate structure (6), automatic cultivate structure (6) set up in the inside that constant temperature cultivated base (1), automatic cultivate structure (6) are located automatic heavy suspension structure (5) and keep away from the one side of automatic centrifugal structure (4) that unfreezes, automatic cultivate structure (6) are used for the stable drive cultivation to stem cell.
2. The automated high-purity stem cell culture equipment according to claim 1, wherein: the constant-temperature cultivation base (1) comprises a base main body (7), a placing support plate (8), a heat storage delivery pipe (9), electric heating resistance wires (10), a first temperature conducting metal rod (11), a refrigeration delivery box (12), a semiconductor refrigeration plate (13) and a second temperature conducting metal rod (14), wherein the top end of the interior of the base main body (7) is fixedly connected with the placing support plate (8), the interior of the base main body (7) is fixedly provided with the first temperature conducting metal rod (11) and the second temperature conducting metal rod (14), the first temperature conducting metal rod (11) and the second temperature conducting metal rod (14) are both positioned at the bottom end of the placing support plate (8), the first temperature conducting metal rod (11) is positioned at the two ends of the heat storage delivery pipe (9), the heat storage delivery pipe (9) is fixed at one end of the base main body (7), and the inner side of the heat storage delivery pipe (9) is fixedly provided with a plurality of electric heating resistance wires (10), refrigeration derivation case (12) is fixed in the other end of base main part (7), the both ends of refrigeration derivation case (12) all lead temperature metal pole (14) fixed connection with the second, the top fixedly connected with semiconductor refrigeration board (13) of refrigeration derivation case (12).
3. The automated high-purity stem cell culture equipment according to claim 1, wherein: the automatic unfreezing centrifugal structure (4) comprises a first movable guide output block (15), a first motor (16), a driving output gear (17), a first driven gear (18), a second driven gear (19), a unfreezing test tube (20), a centrifugal test tube (21), a temperature guide sleeve ring (22) and an electric hot plate (23), wherein the bottom end of the inner side of the first movable guide output block (15) is fixedly connected with the first motor (16) through a screw, the output end of the first motor (16) is fixedly connected with the driving output gear (17), one side of the top end of the first movable guide output block (15) is rotatably connected with the first driven gear (18), the other side of the top end of the first movable guide output block (15) is rotatably connected with the second driven gear (19), and the first driven gear (18) and the second driven gear (19) are both meshed with the driving output gear (17), the top fixedly connected with of first driven gear (18) unfreezes test tube (20), the top fixedly connected with centrifugation test tube (21) of second driven gear (19), the outside fixedly connected with of test tube (20) unfreezes leads the temperature lantern ring (22), the one end of leading the temperature lantern ring (22) is glued fixedly connected with electric plate (23) through leading the temperature.
4. The automated high-purity stem cell culture equipment according to claim 1, wherein: automatic heavy suspension structure (5) are including first reciprocal automatic output structure (24) and heavy suspension test tube (25), the one end fixedly connected with of first reciprocal automatic output structure (24) is heavy suspension test tube (25).
5. The automated high-purity stem cell culture equipment according to claim 4, wherein: the automatic culture structure (6) comprises a second reciprocating automatic output structure (26) and six pore plates (27), and the top end of the second reciprocating automatic output structure (26) is fixedly connected with the six pore plates (27).
6. The automated high-purity stem cell culture equipment according to claim 5, wherein: the structure of the first reciprocating automatic output structure (24) is the same as that of the second reciprocating automatic output structure (26), the first reciprocating automatic output structure (24) and the second reciprocating automatic output structure (26) respectively comprise an internal power block (28), a reserved chute (29), a limiting support plate (30), a second motor (31), an eccentric power plate (32), a reciprocating push guide block (33) and a derivation rod (34), the reserved chute (29) is arranged at one end of the internal power block (28), the limiting support plate (30) is welded inside the internal power block (28), one end of the limiting support plate (30) is fixedly connected with the second motor (31) through a screw, the output end of the second motor (31) is fixedly connected with the eccentric power plate (32), and the bottom end of the eccentric power plate (32) is rotatably connected with the reciprocating push guide block (33), one end of the reciprocating push guide block (33) far away from the eccentric power plate (32) is rotatably connected with a push guide rod (34).
7. The automated high-purity stem cell culture equipment according to claim 6, wherein: the first reciprocating automatic output structure (24) and the second reciprocating automatic output structure (26) also comprise a limit carrying block (35), a linkage guiding block (36), a guide polished rod (37), a matching pushing block (38) and an elastic supporting rod (39), the top end of the built-in power block (28) is fixedly connected with a limit carrying block (35), the top end of the limit carrying block (35) is connected with a linkage leading-out block (36) in a sliding way, both sides of the linkage leading-out block (36) are connected with the limit carrying block (35) through an elastic supporting rod (39), the elastic supporting rod (39) is used for limiting the linkage leading-out block (36) and avoiding the overtravel of the linkage leading-out block (36), a guide polished rod (37) is welded on the inner side of the linkage leading-out block (36), the outer side of the guide polished rod (37) is connected with a matching push block (38) in a sliding way, the top end of the push guide rod (34) is connected with the matching push block (38) in a welding mode.
8. The automated high-purity stem cell culture equipment according to claim 1, wherein: the automatic movable guide liquid taking structure (3) comprises a trackless cylinder (40), a cylinder sliding block (41), a second movable guide output block (42), a third motor (43), a movable guide gear shaft (44), a guide limiting guide block (45), a following rack column (46) and a hydraulic piston cylinder (47), wherein the inner side of the trackless cylinder (40) is connected with the cylinder sliding block (41) in a sliding manner, the second movable guide output block (42) is welded at one end of the top end of the cylinder sliding block (41), the guide limiting guide block (45) is fixedly connected at one end of the second movable guide output block (42), a third motor (43) is fixedly connected to one side of the second movable guide output block (42) through a screw, the movable guide gear shaft (44) is fixedly connected to the output end of the third motor (43), the following rack column (46) is connected to the inner side of the guide limiting guide block (45) in a sliding manner, one end of the movable guide gear shaft (44) is meshed with the following rack column (46), and one end of the top end of the following rack column (46) is fixedly connected with a hydraulic piston cylinder (47) through a screw.
9. The automated high-purity stem cell culture equipment according to claim 8, wherein: the automatic dynamic guide liquid taking structure (3) further comprises a liquid taking column (48), a guide column (49), a fourth motor (50), a screw rod (51), a linkage push rod (52), an extrusion plate (53) and an electromagnetic valve tube (54), wherein the output end of the hydraulic piston cylinder (47) is fixedly connected with the liquid taking column (48), the guide column (49) is welded at the top end of the liquid taking column (48), the fourth motor (50) is fixedly connected at the top end of the guide column (49) through a screw, the screw rod (51) is fixedly connected at the output end of the fourth motor (50), driving grooves are formed in two sides of the guide column (49), the outer side of the screw rod (51) is connected with the linkage push rod (52) through threads, the linkage push rod (52) is in sliding connection with the driving grooves, the extrusion plate (53) is welded at the bottom end of the linkage push rod (52), and the linkage push rod (52) is in sliding connection with the liquid taking column (48), the bottom end of the liquid taking column (48) is fixedly connected with an electromagnetic valve tube (54).
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| CN202110886737.8A CN113667602B (en) | 2021-08-03 | High-purity stem cell culture automation equipment |
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| CN202110886737.8A CN113667602B (en) | 2021-08-03 | High-purity stem cell culture automation equipment |
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