CN113654873A - Microorganism enrichment device and method for microorganism detection - Google Patents
Microorganism enrichment device and method for microorganism detection Download PDFInfo
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- CN113654873A CN113654873A CN202110918851.4A CN202110918851A CN113654873A CN 113654873 A CN113654873 A CN 113654873A CN 202110918851 A CN202110918851 A CN 202110918851A CN 113654873 A CN113654873 A CN 113654873A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/405—Concentrating samples by adsorption or absorption
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
- G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration
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- Biotechnology (AREA)
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- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a microorganism enrichment device and a method for microorganism detection, the microorganism enrichment device for microorganism detection comprises a cup body, a cup opening is provided with a connecting ring, the outer wall of the connecting ring is provided with a first support rod, the first support rod is hinged with a claw, the center of the connecting ring is provided with a top post, the top post is slidably sleeved with a jacking plate, a sliding ring is arranged above the connecting ring, a grid is arranged in the sliding ring, a filter screen is laid on the grid, the inner wall of the sliding ring is longitudinally provided with a first rack, the edge of the jacking plate is vertically provided with a second rack, the first rack and the second rack are in gear transmission, a water bottle is reversely buckled above the sliding ring, the outer wall of the bottle opening is sleeved with a fixed ring, a bottle opening fixed plate is arranged in the water bottle opening fixed plate, a movable plate is arranged above the fixed plate, the center of the movable plate is downwards fixedly provided with a push rod, and a first pressure spring is arranged between the lower end of the push rod and the fixed plate after the push rod passes through the fixed plate, the movable plate, the invention is simple in use, the automatic locking and unlocking of the water bottle and the automatic jacking of the filter screen can be realized.
Description
Technical Field
The invention belongs to the technical field of microorganism detection, and particularly relates to a microorganism enrichment device and method for microorganism detection.
Background
The microorganisms comprise bacteria, fungi, algae and the like, are small in individuals, various in types and close to human relations, and widely relate to the fields of food, medicine, industry and agriculture, environmental protection and the like, and many industries have strict requirements on the content of the microorganisms, and the content of the microorganisms cannot be higher than a certain specified value to meet the specification, so that the content of the microorganisms in a product needs to be detected.
Disclosure of Invention
In order to overcome the technical problems, the invention provides a microorganism enrichment device and a method for microorganism detection.
In order to achieve the purpose, the invention adopts the following technical scheme:
a microorganism enrichment device for microorganism detection comprises a cup body, wherein the cup mouth of the cup body is detachably connected with a connecting ring, the two sides of the outer wall of the connecting ring are symmetrically and respectively fixedly provided with a first supporting rod, the first supporting rods extend outwards and then are bent upwards, and the upper section is hinged with a hook claw, a top column is vertically arranged in the center of the connecting ring upwards through a supporting rod in the connecting ring, the ejection column is sleeved with the ejection plate in a sliding manner, a sliding ring is arranged above the connecting ring in a sliding manner, a third pressure spring is vertically arranged between the connecting ring and the sliding ring, a grid is arranged in the sliding ring, a filter screen is laid on the grid, the center of the grid and the center of the filter screen are penetrated by a top column, a first rack is longitudinally arranged on the inner wall of the sliding ring, a second rack is vertically arranged on the edge of the ejection plate, the first rack and the second rack are in transmission connection through a gear, and the gear is rotatably arranged on the inner wall of the connecting ring.
The device still includes the water-jug, the water-jug is located the slip ring top and inverts, solid fixed ring is established to water-jug bottleneck outer wall cover for with collude the claw cooperation and lock the water-jug, the bottleneck inner wall sets up the fixed plate, the fixed plate top sets up the movable plate, movable plate central authorities downward fixation sets up the ejector pin, the ejector pin passes and sets up first pressure spring between fixed plate back lower extreme and the fixed plate, the fixed plate is opened with the movable plate has a plurality of apertures, and two boards trompil position is complementary, and under the effect of first pressure spring, the movable plate pastes tight fixed plate, seals the water-jug bottleneck, when buckleing the water-jug on the slip ring, the ejector pin can be by the apical post at connecting ring center upwards jack-up, makes movable plate and fixed plate separation to the appearance liquid in the messenger's water-jug flows out.
Preferably, collude the claw and be lever form, the resistance end upwards and inwards form the arch on the top, and the power arm inwards buckles downwards in articulated department, is connected the extension spring between resistance arm and the first support rod top, extension spring effect trend is inwards pulling resistance arm, and resistance arm sets up the joint piece, the first support rod outside corresponds and sets up the joint groove, and when colluding claw power arm and by pushing down, the joint piece is extracted from the joint inslot, colludes claw resistance arm and just inwards rotates under the effect of extension spring and detains the solid fixed ring of water-jug.
Preferably, first branch inboard sets up second branch, the articulated pole that sticks up of second branch, stick up the pole and be the lever form, resistance arm makes progress along first branch, and the power arm is inside to slip ring lower limb below, second pressure spring is connected with first branch root in power arm below, and the slip ring moves down and pushes down and sticks up the pole power arm, and its sticks up the pole resistance arm alright stir and collude claw power arm, makes and colludes claw power arm and outwards expand and reset, with the water-jug unblock.
Preferably, the liftout plate is netted, and fixed surface sets up a plurality of kicking blocks, and the liftout plate rises and to lay the filter screen jack-up above the net, is convenient for take off the filter screen.
Preferably, the sealing ring is arranged on the inner side of the bottle mouth of the water bottle, so that the airtightness between the water bottle and the sliding ring is ensured, a longitudinal sliding groove is reserved on the outer wall of the bottle mouth, and the sliding groove is used for allowing the hook claw power arm to pass through when the enrichment is finished and the unlocking is finished.
A microorganism enrichment method for microorganism detection, comprising the steps of:
in the initial state, sample liquid to be enriched is in the water bottle, the movable plate is tightly attached to the fixed plate, the claw resistance arm is unfolded outwards, and the clamping block is clamped in the clamping port;
s1, a water bottle is reversely buckled on a sliding ring, in the process, the edge of the bottle mouth of the water bottle presses a hook claw power arm downwards, a clamping block on a hook claw resistance arm is pulled out from a clamping opening, a tension spring drives a hook claw to rotate, a bulge at the hook claw resistance end hooks a fixed ring, so that the water bottle is fixed, meanwhile, a mandril is jacked upwards by a jacking column, a first pressure spring is compressed, the mandril drives a movable plate to move upwards to enable the movable plate to be not attached to the fixed plate, the bottle mouth of the water bottle is not sealed, sample liquid flows into a cup body from the water bottle downwards through a filter screen, and microorganisms in the sample liquid are captured and enriched by the filter screen;
s2, after sample liquid completely flows down, rotating the water bottle to enable a sliding groove on the outer side of a bottle opening to be aligned with the hook claw, preventing the bottle opening from hindering the return of the hook claw during unlocking, then pressing the water bottle downwards, pressing the sliding ring downwards by the sealing ring, compressing the third pressure spring, pressing the lower edge of the sliding ring downwards to move the warped rod power arm, compressing the second pressure spring, shifting the warped rod resistance arm inwards and upwards to shift the hook claw power arm, inserting the clamping block into the clamping groove, resetting the hook claw, meanwhile, driving the second rack to move the ejector plate to move upwards through the gear by the first rack, and enabling the ejector block to penetrate through the grid to jack the filter screen upwards;
s3, the unlocked water bottle is taken down, in the process, the sliding ring and the ejector plate return under the action of the third pressure spring, the tilting rod returns under the action of the second pressure spring, the movable plate returns under the action of the first pressure spring, and the filter screen which is ejected out of the sliding ring and is enriched with microorganisms is taken down.
Compared with the prior art, the invention has the beneficial effects that:
1. when the automatic locking device is used, the water bottle filled with sample liquid is reversely buckled on the sliding ring, so that the hook claw can be triggered to automatically lock the water bottle, and meanwhile, the sealing structure formed by the fixed plate and the movable plate in the mouth of the water bottle is automatically opened, so that the sample liquid flows into the cup body from the water bottle through the filter screen;
2. after the enrichment is finished, the water bottle is rotated and then pressed down, so that the locking of the water bottle by the hook claw can be released, and the filter screen is jacked upwards, so that a user can take down the filter screen enriched with microorganisms for next operation.
Drawings
FIG. 1 is an overall cross-sectional view of the present invention;
FIG. 2 is a schematic view of the present invention when the water bottle is locked;
FIG. 3 is a schematic view at A in FIG. 1;
FIG. 4 is a schematic view at B in FIG. 2;
fig. 5 is a schematic view of the mouth of a water bottle.
Description of reference numerals:
1, a cup body; 2 connecting rings; 3, a slip ring; 4 a first strut; 5, jacking a column; 6, ejecting the plate; 6-1, a top block; 7, grid; 8, filtering by using a filter screen; 9 water bottles; 9-1 chute; 10 fixing the plate; 11 moving the board; 12, a top rod; 13 a first compression spring; 14 fixing the ring; 15, sealing rings; 16 a first rack; 17 gears; 18 a second rack; 19 hooking claws; 20 tension springs; 21, a clamping block; 22, raising a rod; 23 a second strut; 24 a second compression spring; 25 a third pressure spring; 26 snap into the groove.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the accompanying drawings are illustrative only for the purpose of explaining the present invention, and are not to be construed as limiting the present invention.
The utility model provides a microorganism enrichment device for microbiological detection, includes cup 1, connection go-between 2 can be dismantled to 1 rim of a cup of cup, each fixed first branch 4 that sets up in go-between 2 outer wall both sides, the articulated claw 19 that colludes of first branch 4, go-between 2 center sets up fore-set 5, fore-set 5 slides and cup joints liftout plate 6, go-between 2 top slides and sets up slip ring 3, set up net 7 in the slip ring 3, lay filter screen 8 on the net 7, 3 inner walls of slip ring vertically set up first rack 16, 6 marginal vertical second racks 18 that set up of liftout plate, be connected through the gear 17 transmission between first rack 16 and the second rack 18, gear 17 rotates and sets up in go-between 2 inner walls.
The device still includes water-jug 9, water-jug 9 is located 3 tops of slip ring and inverts, solid fixed ring 14 is established to water-jug 9 bottleneck outer wall cover, sets up fixed plate 10 in the bottleneck, fixed plate 10 top sets up movable plate 11, 11 central authorities of movable plate are the fixed ejector pin 12 that sets up downwards, ejector pin 12 passes and sets up first pressure spring 13 between fixed plate 10 back lower extreme and the fixed plate 10.
Collude claw 19 and be the lever form, the resistance end upwards and inwards form the arch on the top, and the power arm inwards buckles downwards in articulated department, is connected extension spring 20 between resistance arm and 4 tops of first bracing piece, and the resistance arm sets up joint piece 21.
The outer side of the first supporting rod 4 is provided with a clamping groove 26.
Inboard internal fixation in first branch 4 sets up second branch 23, the articulated stick up 22 of second branch 23, stick up 22 for the lever form, the resistance arm is upwards along first branch 4, and the power arm is inwards to 3 lower limb belows of slip ring, the vertical second pressure spring 24 of being connected in power end below and 4 roots of first branch.
The ejector plate 6 is net-shaped, and the upper surface of the ejector plate is fixedly provided with a plurality of ejector blocks 6-1.
The fixed plate 10 and the movable plate 11 are provided with a plurality of small holes, and the hole positions of the two plates are complementary.
And a third pressure spring 25 is vertically arranged at the sliding connection position of the connecting ring 2 and the sliding ring 3.
The inner side of the bottle mouth of the water bottle 9 is provided with a sealing ring 15, and the outer wall of the bottle mouth is provided with a longitudinal sliding groove 9-1.
A microorganism enrichment method for microorganism detection, comprising the steps of:
in the initial state, sample liquid to be enriched is in the water bottle 9, the movable plate 11 is tightly attached to the fixed plate 10, the resistance arm of the claw 19 is unfolded outwards, and the clamping block 21 is clamped in the clamping port;
s1, a water bottle 9 is reversely buckled on a sliding ring 3, in the process, the edge of the bottle opening of the water bottle 9 presses a power arm of a hook claw 19 downwards, a clamping block 21 on a resistance arm of the hook claw 19 is pulled out of a clamping opening, a tension spring 20 drives the hook claw 19 to rotate, a bulge at the resistance end of the hook claw 19 hooks a fixing ring 14, so that the water bottle 9 is fixed, meanwhile, a mandril 12 is jacked upwards by a jacking column 5, a first pressure spring 13 is compressed, the mandril 12 drives a movable plate 11 to move upwards to enable the movable plate 11 to be out of fit with a fixing plate 10, the bottle opening of the water bottle 9 is unsealed, sample liquid flows into a cup body 1 from the water bottle 9 downwards through a filter screen 8, and microorganisms in the sample liquid are captured and enriched by the filter screen 8;
s2, after sample liquid completely flows down, rotating the water bottle 9 to enable a chute 9-1 on the outer side of a bottle opening to be aligned with the hook claw 19, preventing the bottle opening from hindering the recovery of the hook claw 19 during unlocking, then pressing the water bottle 9 downwards, pressing the sliding ring 3 downwards by the sealing ring 15, compressing the third pressure spring 25, pressing the rocker 22 power arm downwards by the lower edge of the sliding ring 3, compressing the second pressure spring 24, shifting the hook claw 19 power arm inwards and upwards by the rocker 22 resistance arm, inserting the clamping block 21 into the clamping groove 26, resetting the hook claw 19, meanwhile, driving the second rack 18 to drive the ejector plate 6 to move upwards by the first rack 16 through the gear 17, and enabling the ejector block 6-1 to penetrate through the grid 7 to jack the filter screen 8 upwards;
s3, the unlocked water bottle 9 is taken down, in the process, the sliding ring 3 and the ejection plate 6 return under the action of a third pressure spring 25, the tilting rod 22 returns under the action of a second pressure spring 24, the moving plate 11 returns under the action of a first pressure spring 13, and the filter screen 8 which is ejected out of the sliding ring 3 and is enriched with microorganisms is taken down.
While embodiments of the invention have been shown and described, it will be understood by those of ordinary skill in the art that: various changes, modifications, substitutions and alterations can be made to the above embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.
Claims (10)
1. A microorganism enrichment device for microorganism detection, includes the cup, its characterized in that: the cup rim of a cup sets up the go-between, the go-between outer wall is fixed to be set up a plurality of first branches, the articulated claw that colludes of first branch, the go-between center sets up the fore-set, the fore-set slides and cup joints the liftout plate, it slides and sets up the slip ring to go-between top, set up the net in the slip ring, lay the filter screen on the net, the slip ring inner wall vertically sets up first rack, the vertical second rack that sets up in liftout plate edge, be connected through gear drive between first rack and the second rack, gear revolve sets up in the go-between inner wall.
2. The microorganism enrichment apparatus for microorganism detection as claimed in claim 1, wherein: the novel water bottle is characterized by further comprising a water bottle, the water bottle is located above the sliding ring and is inverted, the outer wall of the bottle opening of the water bottle is sleeved with a fixing ring, a fixing plate is arranged in the bottle opening, a moving plate is arranged above the fixing plate, an ejector rod is fixedly arranged in the center of the moving plate downwards, and a first pressure spring is arranged between the lower end of the ejector rod and the fixing plate after the ejector rod penetrates through the fixing plate.
3. The microorganism enrichment apparatus for microorganism detection as claimed in claim 1, wherein: collude the claw and be the lever form, the resistance end upwards and inwards form the arch on the top, the power arm inwards, buckles down in articulated department, is connected the extension spring between resistance arm and the first bracing piece top, and the resistance arm sets up the joint piece.
4. The microorganism enrichment apparatus for microorganism detection as claimed in claim 1, wherein: the clamping groove is formed in the outer side of the first supporting rod.
5. The microorganism enrichment apparatus for microorganism detection as claimed in claim 1, wherein: the inboard second branch that sets up of first branch, the articulated pole that sticks up of second branch, stick up the pole and be the lever form, resistance arm makes progress along first branch, and the power arm is inside to slip ring lower limb below, the second pressure spring is connected with first branch root in power arm below.
6. The microorganism enrichment apparatus for microorganism detection as claimed in claim 1, wherein: the ejector plate is net-shaped, and the upper surface is fixedly provided with a plurality of ejector blocks.
7. A microorganism enrichment apparatus for microorganism detection as claimed in claim 2, wherein: the fixed plate and the movable plate are provided with a plurality of small holes, and the hole positions of the two plates are complementary.
8. The microorganism enrichment apparatus for microorganism detection as claimed in claim 1, wherein: and a third pressure spring is arranged at the sliding connection position of the connecting ring and the sliding ring.
9. A microorganism enrichment apparatus for microorganism detection as claimed in claim 2, wherein: the inner side of the bottle mouth of the water bottle is provided with a sealing ring, and the outer wall of the bottle mouth is provided with a longitudinal sliding groove.
10. A microorganism enrichment method for microorganism detection, which is characterized in that the microorganism enrichment apparatus for microorganism detection according to any one of claims 1 to 9 is used, and comprises the following steps:
in the initial state, sample liquid to be enriched is in the water bottle, the movable plate is tightly attached to the fixed plate, the claw resistance arm is unfolded outwards, and the clamping block is clamped in the clamping port;
s1, reversely buckling a water bottle on a sliding ring, pressing a hook claw power arm downwards at the edge of a bottle opening of the water bottle in the process, pulling out a clamping block from a clamping opening, driving a hook claw to rotate by a tension spring to match with a fixed ring to lock the water bottle, meanwhile, jacking a jacking rod upwards by a jacking column, moving a moving plate upwards, enabling sample liquid to flow into a cup body from the water bottle, and enriching microorganisms in a filter screen;
s2, after enrichment is completed, rotating the water bottle to align a chute on the outer side of the bottle opening with the hook claw, pressing the water bottle downwards, pressing the sliding ring downwards by the sealing ring, pressing the warping rod on the lower edge of the sliding ring to stir the power arm of the hook claw to reset the hook claw, and simultaneously driving the second rack to move the ejector plate upwards through the gear to jack the filter screen;
s3, the water bottle is taken down, each part is reset under the action of the first pressure spring, the second pressure spring or the third pressure spring, and the filter screen enriched with microorganisms is taken down.
Priority Applications (1)
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CN202110918851.4A CN113654873A (en) | 2021-08-11 | 2021-08-11 | Microorganism enrichment device and method for microorganism detection |
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CN202110918851.4A CN113654873A (en) | 2021-08-11 | 2021-08-11 | Microorganism enrichment device and method for microorganism detection |
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CN113654873A true CN113654873A (en) | 2021-11-16 |
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CN202110918851.4A Withdrawn CN113654873A (en) | 2021-08-11 | 2021-08-11 | Microorganism enrichment device and method for microorganism detection |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP4202033A1 (en) * | 2021-12-22 | 2023-06-28 | Pall Corporation | Filtration assembly |
-
2021
- 2021-08-11 CN CN202110918851.4A patent/CN113654873A/en not_active Withdrawn
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP4202033A1 (en) * | 2021-12-22 | 2023-06-28 | Pall Corporation | Filtration assembly |
US11814667B2 (en) | 2021-12-22 | 2023-11-14 | Cytiva Us Llc | Filtration assembly |
JP7416386B2 (en) | 2021-12-22 | 2024-01-17 | ポール・コーポレーション | filtration assembly |
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Application publication date: 20211116 |