Disclosure of Invention
The embodiment of the invention provides a high-efficiency biological information data processing method and a high-efficiency biological information data processing system, and aims to solve the problems that the relevance among various types of microbial data information is not strong, the recombination application of data and the tracking of a data source are not easy, and the change analysis of microbes in intestinal substances needs to span a long time period, so that the efficiency of data processing and utilization is low, and more manpower is consumed in the conventional mode of manually recording or manually inputting research and analysis data of intestinal microbes.
The embodiment of the invention is realized in such a way that, on one hand, a high-efficiency biological information data processing method comprises the following steps:
acquiring an intestinal substance sample, and marking and storing the intestinal substance sample; the markers at least comprise an intestinal substance sampling time and an intestinal substance sampling position;
analyzing the microbial species and the microbial quantity contained in the marked intestinal substance sample;
storing the analysis data in a database corresponding to the intestinal substance sample markers;
calling historical intestinal substance sample analysis data stored in a database to compare in the longitudinal direction to obtain a comparison result;
and predicting the ecological change of the intestinal microorganisms according to the comparison result, and feeding the prediction information back to the client.
As a modified scheme of the invention: the method for obtaining the intestinal substance sample and marking and storing the intestinal substance sample specifically comprises the following steps:
calling a sampling equipment list required by the intestinal substance sample and an operation step of collecting the intestinal substance sample, and sampling the intestinal substance;
classifying and storing the intestinal substance samples obtained by sampling, and orderly marking the intestinal substance samples classified and stored;
the storage environment of the sample of intestinal material is monitored to maintain the storage environment at a value suitable for the survival of the intestinal microorganisms or a storage condition capable of storing the intestinal microorganisms for a prescribed period of time.
As a further improvement of the invention: the method further comprises the following steps:
judging whether the storage time of the intestinal substance sample is greater than the effective time value or not;
when the storage time of the intestinal substance sample is greater than the effective time value, transmitting intestinal substance sample destruction information to the client;
when the storage time of the intestinal substance sample is not more than the effective time value, further judging whether the storage environment meets the storage condition value;
when the storage environment meets the storage condition value, the stored intestinal substance sample can be continuously used;
and when the storage environment does not meet the storage condition value, sending the intestinal substance sample destruction information to the client.
As a further scheme of the invention: the calling of the historical intestinal substance sample analysis data stored in the database is carried out in the longitudinal direction for comparison, and the comparison result specifically comprises the following steps:
calling intestinal substance sample analysis data of the same sampling position at different time points;
counting the number of the microbial species obtained by analyzing the intestinal substance samples on a microbial species trend graph according to a time sequence;
under the condition of keeping the current change trend of the internal environment of the intestinal tract, predicting the future amount of the intestinal microorganisms at the sampling position according to the trend chart of the types of the microorganisms;
transmitting the predicted future amount of the gut microbes to the client.
As a further scheme of the invention: after the data of the intestinal substance sample analysis at different time points of the same sampling position are called, the method further comprises the following steps:
counting the number of the same microorganism species obtained by analyzing the intestinal substance samples on a microorganism number trend graph according to a time sequence;
predicting the number value of the microorganism to be reached in the future according to the microorganism number trend graph;
and feeding back the predicted microorganism name and the corresponding future quantity value to the client.
As an optimization scheme of the invention: the calling of the historical intestinal substance sample analysis data stored in the database is carried out in the longitudinal direction for comparison, and the comparison result specifically comprises the following steps:
calling intestinal substance sample analysis data of different positions at the same sampling time point;
counting the types of microorganisms and the number of each microorganism on a position difference trend chart according to the specified sampling position sequence;
and analyzing to obtain the most suitable survival position of each microorganism and the distribution change condition of the microorganism according to the position difference trend graph.
As another scheme of the invention: after predicting the ecological change of the intestinal microorganisms according to the comparison result and feeding the prediction information back to the client, the method further comprises the following steps:
acquiring intestinal environment factors of a sampling position;
analyzing to obtain main influence factors by combining the ecological change condition of the intestinal microorganisms;
and feeding back the main influence factors and the intestinal tract maintenance suggestions to the client.
In another aspect, a high efficiency bioinformation data processing system includes:
the collection end is used for obtaining the intestinal substance sample and marking and storing the intestinal substance sample; the markers at least comprise an intestinal substance sampling time and an intestinal substance sampling position;
the analysis end is used for analyzing the microbial species and the microbial quantity contained in the marked intestinal substance sample;
the storage module is used for correspondingly storing the analysis data and the intestinal substance sample markers in the database;
the comparison module is used for calling the historical intestinal substance sample analysis data stored in the database to compare in the longitudinal direction to obtain a comparison result;
and the prediction module is used for predicting the ecological change of the intestinal microorganisms according to the comparison result and feeding the prediction information back to the client.
The invention has the beneficial effects that: analyzing the types and the quantity of microorganisms contained in the marked intestinal substance sample, and storing the analysis data and the intestinal substance sample mark in a database correspondingly; and when the data are accumulated to a certain degree, calling historical intestinal substance sample analysis data stored in the database to compare in the longitudinal direction, predicting the ecological change of the intestinal microorganisms according to the comparison result, and feeding the prediction information back to the client. The client further infers the change trend of the intestinal microorganisms and the internal environment of the intestinal tract according to the prediction information, and further infers the physical health condition of the testee. The method and the system can track the intestinal microorganism information data in a full flow, so that the data have a front-back comparison relation, data and feedback information are automatically input, the labor amount of manpower is reduced, and the data processing speed is more efficient. The problem of current research and analysis to intestinal microorganism data adopt artificial record or artificial input mode inefficiency is solved, still solved the associativity between various microorganism data information not strong, and the reconsolidation of data is used and the pursuit of data source is difficult for the efficiency of data processing is lower, consumes more manpower is solved.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The invention analyzes the microorganism species and the microorganism quantity contained in the marked intestinal substance sample, and stores the analysis data and the intestinal substance sample mark in a database correspondingly; and when the data are accumulated to a certain degree, calling historical intestinal substance sample analysis data stored in the database to compare in the longitudinal direction, predicting the ecological change of the intestinal microorganisms according to the comparison result, and feeding the prediction information back to the client. The client further infers the change trend of the intestinal microorganisms and the internal environment of the intestinal tract according to the prediction information, and further infers the physical health condition of the testee. The method and the system can track the intestinal microorganism information data in a full flow, so that the data have a front-back comparison relation, data and feedback information are automatically input, the labor amount of manpower is reduced, and the data processing speed is more efficient. The problem of current research and analysis to intestinal microorganism data adopt artificial record or artificial input mode inefficiency is solved, still solved the associativity between various microorganism data information not strong, and the reconsolidation of data is used and the pursuit of data source is difficult for the efficiency of data processing is lower, consumes more manpower is solved.
Fig. 1 is a schematic diagram showing an operating environment of a high-efficiency biological information data processing method according to an embodiment of the present invention, in which an acquisition end 100 acquires an intestinal substance sample, in order to study distribution of microorganisms at different locations of the intestinal tract and at different time periods at the same location, multiple acquisitions at different locations and at different time points of the intestinal tract are required, a part of the intestinal substance sample is stored, another part of the intestinal substance sample is transferred to an analysis end 200 for further analysis, storage information of the intestinal substance sample and analysis data of the microorganisms contained in the intestinal substance sample are transferred to an integration module, the integration module stores data in a database 1, each module in the integration module processes the stored intestinal substance sample data and the biological information data of the microorganisms analyzed in the intestinal substance sample, and feeds back a processing result to a client 2, the client 2 deeply studies the change of the internal environment of the intestinal tract according to the feedback of the change condition of the intestinal microorganisms.
Fig. 2 is a main flow chart of a high-efficiency biological information data processing method according to an embodiment of the present invention, the method including:
step S10: and acquiring an intestinal substance sample, and marking and storing the intestinal substance sample. The markers include at least an intestinal material sampling time and an intestinal material sampling location. Gather end 100 and gather intestinal material, gather end 100 including gathering the instrument, splendid attire instrument, stock solution etc.. In addition, when a sample of intestinal material is taken, multiple samples of intestinal material are typically taken from the same location to obtain multiple samples, some of which are used for analysis and others of which are stored for later use.
Step S11: the type and number of microorganisms contained in the labeled intestinal material sample are analyzed. At the analysis end 200, the intestinal substance sample is subjected to microbial flora diversity analysis by a denaturing gradient gel electrophoresis (PCR-DGGE) method to obtain the type and the number of microorganisms.
Step S12: storing the analytical data in a database in correspondence with the intestinal material sample markers.
Step S13: and calling historical intestinal substance sample analysis data stored in the database to compare in the longitudinal direction to obtain a comparison result. The longitudinal comparison refers to comparing and analyzing all data in a long time period, or analyzing the intestinal substance sample analysis data corresponding to the intestinal substance samples in a plurality of positions.
Step S14: and predicting the ecological change of the intestinal microorganisms according to the comparison result, and feeding the prediction information back to the client. The prediction of the ecological changes of the microorganisms in the intestinal substances is actually the prediction of the health condition of the intestinal tract around the microorganisms.
In one aspect of this embodiment, the obtaining a sample of intestinal substance, and the marking and storing the sample of intestinal substance specifically includes:
step S100: and calling a sampling equipment list required by the intestinal substance sample and an operation step of collecting the intestinal substance sample, and sampling the intestinal substance. The collection equipment list refers to equipment and consumptive material lists such as intestinal microorganism collection, required consumptive material of storage and stock solution, and wherein the memotron is aseptic product with the collection spoon, and the pollution of microorganism and microorganism residual DNA to the sample in the at utmost has reduced the consumptive material.
Step S101: and classifying and storing the intestinal substance samples obtained by sampling, and orderly marking the intestinal substance samples classified and stored.
Step S102: the storage environment of the sample of intestinal material is monitored to maintain the storage environment at a value suitable for the survival of the intestinal microorganisms or a storage condition capable of storing the intestinal microorganisms for a prescribed period of time. Samples of intestinal material can sometimes be stored under ambient conditions. Sometimes, samples of intestinal material are directly immersed in the storage solution, and in order to protect the microorganisms in the samples, the storage solution of intestinal material samples needs to be stored at-20 ℃ and-80 ℃, and repeated freezing and thawing of the samples should be avoided during storage and transportation.
Fig. 3 shows a flow chart of monitoring storage of intestinal substance samples in a high-efficiency bioinformatic data processing method according to an embodiment of the present invention, the method further includes:
step S20: and judging whether the storage time of the intestinal substance sample is greater than the effective time value. Generally, intestinal substance samples immersed in a storage solution are stored at low temperature, the storage period is about 10 days, after 10 days, the microbial activity is reduced, the amount of the intestinal substance samples is gradually reduced at low temperature, the actual information of the samples is lost, and the intestinal substance samples cannot be used again.
Step S21: and when the storage time of the intestinal substance sample is greater than the effective time value, sending intestinal substance sample destruction information to the client.
Step S22: and when the storage time of the intestinal substance sample is not more than the effective time value, further judging whether the storage environment meets the storage condition value. Some samples need to be stored at normal temperature, and some samples need to be stored at low temperature, and for whether the storage conditions are stable, close attention needs to be paid to prevent the samples in storage from changing due to environmental changes, so that the displayed sample data are inconsistent with the original sample data.
Step S23: when the storage environment meets the storage condition value, the stored intestinal substance sample can be used continuously.
Step S24: and when the storage environment does not meet the storage condition value, sending the intestinal substance sample destruction information to the client.
Fig. 4 shows a flow chart of longitudinal analysis of historical data in a high-efficiency biological information data processing method according to an embodiment of the present invention, where the calling of the historical intestinal substance sample analysis data stored in the database is performed in a longitudinal comparison, and the obtaining of the comparison result specifically includes:
step S40: and calling intestinal substance samples at different time points of the same sampling position to analyze data.
Step S41: and counting the number of the microbial species obtained by analyzing the intestinal substance samples on a microbial species trend chart according to the time sequence.
Step S42: and under the condition of keeping the current change trend of the internal environment of the intestinal tract, predicting the future amount of the intestinal microorganisms at the sampling position according to the trend chart of the types of the microorganisms. The change situation of the microbial species along with time can be obtained from the trend graph of the microbial species, and the succession of the microbes is predicted along with the continuous change of the current internal environment of the intestinal tract, wherein the succession can be good development or bad development.
Step S43: transmitting the predicted future amount of the gut microbes to the client. And the client deduces the future change of the internal environment of the intestinal tract according to the prediction result and guides a user of the client to timely maintain and treat the internal environment of the intestinal tract.
In one aspect of this embodiment, after the retrieving the analysis data of the intestinal substance samples at different time points of the same sampling location, the method further includes:
step S50: the number of the same microorganism species obtained by analyzing intestinal substance samples is counted on a microorganism number trend graph according to the time sequence.
Step S51: and predicting the number value of the microorganism to be reached in the future according to the microorganism number trend graph.
Step S52: and feeding back the predicted microorganism name and the corresponding future quantity value to the client. The slope of the decay of a single microorganism over time can be used to measure the survival of a microbial community, such as temperature sensitive microorganisms that may disappear or decrease in amount when the temperature of the environment inside the intestinal tract changes to a greater extent. Therefore, the analysis of the number change of single microorganisms can also analyze the specific reasons of the change of the internal environment of the intestinal tract, so that the analysis is more specific and targeted.
In another case of this embodiment, the comparing the analysis data of the historical intestinal substance samples stored in the calling database in the longitudinal direction, and the obtaining of the comparison result specifically includes:
step S60: and calling intestinal substance samples at different positions at the same sampling time point to analyze data.
Step S61: the microorganism species and the number of each microorganism were counted on a position difference trend chart in the order of the designated sampling positions.
Step S62: and analyzing to obtain the most suitable survival position of each microorganism and the distribution change condition of the microorganism according to the position difference trend graph. The change of the position of the intestinal substance can cause the change of the intestinal microorganisms, the environmental condition that the microorganisms are suitable for living can be deduced according to the change condition of the microorganisms, and the microorganisms are cultured in a targeted manner; or creating environmental conditions suitable for the survival of the microorganisms at local positions inside the intestinal tract which are not suitable for the survival of the microorganisms so as to facilitate the microorganisms with positive effects to play roles in different places.
In one aspect of the embodiment of the present invention, after predicting the ecological change of the intestinal microbes according to the comparison result and feeding back the prediction information to the client, the method further includes:
step S70: and acquiring the intestinal environment factors of the sampling position.
Step S71: and analyzing to obtain main influence factors by combining the ecological change condition of the intestinal microorganisms.
Step S72: and feeding back the main influence factors and the intestinal tract maintenance suggestions to the client. The survival of the microorganisms changes along with the increase/decrease of the temperature; further analysis of the relationship between the succession of microorganisms and the environmental factors in the intestinal tract, the diversity of the microbial community may be related to the physical and chemical properties in the intestinal tract.
Fig. 5 is a schematic diagram illustrating an internal structure of a high-efficiency biological information data processing system according to an embodiment of the present invention, the system including:
and the collection end 100 is used for obtaining the intestinal substance sample and marking and storing the intestinal substance sample. The markers include at least an intestinal material sampling time and an intestinal material sampling location.
And an analysis end 200 for analyzing the kind and the number of microorganisms contained in the marked intestinal substance sample.
The storage module 300 is configured to store the analysis data in the database in correspondence with the intestinal substance sample markers.
The comparison module 400 is configured to call the historical intestinal substance sample analysis data stored in the database to perform comparison in the longitudinal direction, so as to obtain a comparison result.
And the prediction module 500 is used for predicting the ecological change of the intestinal microorganisms according to the comparison result and feeding the prediction information back to the client.
In order to load the above method and system to operate successfully, the system may include more or less components than those described above, or combine some components, or different components, in addition to the various modules described above, for example, input/output devices, network access devices, buses, processors, memories, and the like.
The Processor may be a Central Processing Unit (CPU), other general purpose Processor, a Digital Signal Processor (DSP), an Application Specific Integrated Circuit (ASIC), an off-the-shelf Programmable Gate Array (FPGA) or other Programmable logic device, discrete Gate or transistor logic, discrete hardware components, etc. The general purpose processor may be a microprocessor or the processor may be any conventional processor or the like, which is the control center of the client or system and which connects the various parts of the overall user terminal using various interfaces and lines.
The memory may be used to store a computer and system programs and/or modules, and the processor may implement various functions of the client by running or executing the computer programs and/or modules stored in the memory and calling data stored in the memory. The memory mainly comprises a storage program area and a storage data area, wherein the storage program area can store an operating system, application programs (such as an information acquisition template display function, a product information publishing function and the like) required by at least one function and the like; the storage data area may store data created according to the use of the berth-state display system (e.g., product information acquisition templates corresponding to different product types, product information that needs to be issued by different product providers, etc.), and the like. In addition, the memory may include high speed random access memory, and may also include non-volatile memory, such as a hard disk, a memory, a plug-in hard disk, a Smart Media Card (SMC), a Secure Digital (SD) Card, a Flash memory Card (Flash Card), at least one magnetic disk storage device, a Flash memory device, or other volatile solid state storage device.
It should be understood that, although the steps in the flowcharts of the embodiments of the present invention are shown in sequence as indicated by the arrows, the steps are not necessarily executed in sequence as indicated by the arrows. The steps are not performed in the exact order shown and described, and may be performed in other orders, unless explicitly stated otherwise. Moreover, at least a portion of the steps in various embodiments may include multiple sub-steps or multiple stages that are not necessarily performed at the same time, but may be performed at different times, and the order of performance of the sub-steps or stages is not necessarily sequential, but may be performed in turn or alternately with other steps or at least a portion of the sub-steps or stages of other steps.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.