CN113617225B - Device and method for cleaning exogenous viruses in animal serum - Google Patents
Device and method for cleaning exogenous viruses in animal serum Download PDFInfo
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- CN113617225B CN113617225B CN202110822178.4A CN202110822178A CN113617225B CN 113617225 B CN113617225 B CN 113617225B CN 202110822178 A CN202110822178 A CN 202110822178A CN 113617225 B CN113617225 B CN 113617225B
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- 238000004140 cleaning Methods 0.000 title claims abstract description 76
- 210000002966 serum Anatomy 0.000 title claims abstract description 66
- 241000700605 Viruses Species 0.000 title claims abstract description 35
- 241001465754 Metazoa Species 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims description 9
- 238000001914 filtration Methods 0.000 claims abstract description 62
- 239000012528 membrane Substances 0.000 claims abstract description 40
- 238000007789 sealing Methods 0.000 claims abstract description 33
- 230000000694 effects Effects 0.000 claims abstract description 17
- 230000007246 mechanism Effects 0.000 claims description 33
- 210000002381 plasma Anatomy 0.000 claims description 21
- 238000002156 mixing Methods 0.000 claims description 9
- 239000000243 solution Substances 0.000 claims description 9
- 108090000190 Thrombin Proteins 0.000 claims description 6
- 230000029087 digestion Effects 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 6
- 238000004806 packaging method and process Methods 0.000 claims description 6
- 229960004072 thrombin Drugs 0.000 claims description 6
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 3
- 230000009471 action Effects 0.000 claims description 3
- 229940037003 alum Drugs 0.000 claims description 3
- 230000008014 freezing Effects 0.000 claims description 3
- 238000007710 freezing Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 239000011259 mixed solution Substances 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims 3
- 230000010405 clearance mechanism Effects 0.000 description 8
- 239000012982 microporous membrane Substances 0.000 description 5
- 238000009434 installation Methods 0.000 description 4
- 230000001147 anti-toxic effect Effects 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000011045 prefiltration Methods 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 239000002033 PVDF binder Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000002657 fibrous material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- -1 polypropylene Polymers 0.000 description 2
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 2
- 239000004743 Polypropylene Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000003365 glass fiber Substances 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229920005594 polymer fiber Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/14—Blood; Artificial blood
- A61K35/16—Blood plasma; Blood serum
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/0005—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts
- A61L2/0011—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts using physical methods
- A61L2/0017—Filtration
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
- A61L2202/20—Targets to be treated
- A61L2202/22—Blood or products thereof
Abstract
The invention relates to a cleaning device for exogenous viruses in animal serum, which comprises a baseplate frame, supporting columns, a baseplate frame, cleaning components and sealing frames, wherein the supporting columns are uniformly arranged at the upper ends of the baseplate frame, the baseplate frame is arranged between the upper ends of the supporting columns, the cleaning components are arranged at the upper ends of the baseplate frame through screws, and the sealing frames are arranged at the upper ends of the cleaning components through screws. The invention can solve the problems that the existing equipment has poor filtering effect when filtering and cleaning viruses, can not effectively protect the filtering membrane, causes the phenomenon that the filtering membrane is easy to damage due to deformation when being pressed, has poor mounting and dismounting effects of the filtering membrane when filtering and cleaning viruses, can not timely clean the surface of the filtering membrane, can not adjust the number of the filtering membrane according to the use requirement, affects the filtering effect of serum and the like.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a device and a method for cleaning exogenous viruses in animal serum.
Background
For antitoxin and antiserum biological products from animal serum, the purification process is extremely complex and the quality control is difficult, so that the removal and inactivation of viruses are very important in the production process of the antitoxin and antiserum with animal blood as a raw material, and only then the safety of the antitoxin and antiserum biological products can be ensured.
At present, the existing animal serum exogenous viruses generally have the following defects in the cleaning process: 1. when the existing equipment is used for filtering and cleaning viruses, the filtering effect is poor, the filtering effect is insufficient, the filtering membrane cannot be effectively protected, deformation is easy to occur when the filtering membrane is pressed, and damage is easy to occur to the filtering membrane; 2. when the existing equipment filters and cleans viruses, the installation and disassembly effects of the filtering membrane are poor, the surface of the filtering membrane cannot be cleaned timely, the number of the filtering membrane cannot be adjusted according to the use requirement, and the filtering effect of serum is affected.
Disclosure of Invention
In order to solve the problems, the invention provides a cleaning device for foreign viruses in animal serum, which can solve the problems in the background art.
In order to achieve the above purpose, the present invention is implemented by adopting the following technical scheme: the utility model provides a cleaning device of animal serum exogenous virus, includes underframe frame, support column, base frame, clearance subassembly and sealing frame, the underframe frame upper end evenly install the support column, install the base frame between the support column upper end, the clearance subassembly is installed through the screw to the base frame upper end, the sealing frame is installed through the screw to the clearance subassembly upper end.
The cleaning assembly comprises a shell frame, a connecting frame, a mounting frame, a positioning frame, a locking mechanism and a cleaning mechanism, wherein the shell frame is of a cylindrical structure, an open slot is uniformly formed in the outer side of the shell frame, the connecting frame is symmetrically arranged in the shell frame, the connecting frame is of a cross structure, the mounting frame is arranged between the connecting frames, sliding grooves are formed in the mounting frame and the shell frame, the positioning frame is arranged between the sliding grooves, the locking mechanism is arranged in the positioning frame and fixedly clamped with the sliding grooves, the cleaning mechanism is arranged in the open slot, the middle of the cleaning mechanism is connected with the positioning frame, and the inner side of the cleaning mechanism is tightly attached to the mounting frame.
The cleaning mechanism comprises a cleaning frame, a handle frame, a connecting plate and a filtering frame, wherein the cleaning frame is of an arc-shaped structure and is matched with the opening groove, mounting holes are uniformly formed in the cleaning frame, the handle frame is mounted in the middle of the outer side of the cleaning frame, the connecting plate is uniformly mounted on the concave surface of the cleaning frame, the position of the connecting plate is adjustable, and the filtering frame is mounted in the middle of the connecting plate.
The filter frame comprises two bearing plates and filter membranes, wherein the filter membranes are arranged between the bearing plates, and the two bearing plates are connected through screws.
Further, the base frame be rectangular structure, the base frame is connected with the clearance subassembly through the screw, the fluid-discharge tube is installed to base frame lower extreme.
Further, the sealing frame upper end be arc structure, the sealing frame is connected with the clearance subassembly through the screw, sealing frame upper end is provided with the feed liquor pipe, is provided with the manometer on the feed liquor pipe, the manometer can accurately show the inside pressure intensity of clearance subassembly.
Further, the cross section of mounting bracket be cross structure, the symmetry is provided with sealed pad on the mounting bracket lateral surface, sealed pad is made for wear-resisting rubber material.
Furthermore, the cross section of locating rack be I shape structure, locating rack mid-mounting has locking mechanism, and the locating rack outside symmetry is provided with the draw-in groove, evenly installs the sealing strip in the draw-in groove.
Further, locking mechanism include regulation pole, briquetting, wedge, guide bar, extension spring and pressure strip, the locating rack middle part be connected with the regulation pole through screw-thread fit's mode, the regulation pole outside is provided with the screw, adjust the inboard briquetting of installing of pole, the locating rack is inside to be provided with logical groove, lead to the inslot symmetry and be provided with the guide bar, be connected with the guide bar through sliding fit's mode in the guide bar, the wedge is installed to the guide bar inboard, the wedge is hugged closely with the briquetting, is provided with extension spring on the guide bar, the pressure strip is installed in the guide bar outside.
Further, the pressure plate be protruding type structure, pressure plate and spout cooperate, evenly are equipped with the non-slip raised on the pressure plate lateral surface, the non-slip raised is regular wave structure.
Further, the bearing plate is of a fan-shaped structure, arc-shaped grooves are uniformly formed in the outer side face of the bearing plate, and round holes are uniformly formed in the arc-shaped grooves.
Further, the filtering membrane is a biological permeable membrane, the outer side of the filtering membrane is clung to the inner side surface of the bearing plate, and the filtering membrane is uniformly provided with filtering holes.
In addition, the invention also provides a cleaning method of the cleaning device for the exogenous viruses of the animal serum, which specifically comprises the following steps:
s1, filtering plasma: balancing the frozen animal blood plasma to room temperature, filtering the blood plasma by medical gauze, and collecting the filtered filtrate;
s2, adding thrombin: uniformly mixing thrombin solution and calcium chloride solution according to a certain proportion for 28-32 minutes, and then adding the mixed solution into the filtrate in the step S1;
s3, mixing and digestion: carrying out mixed digestion on the plasma after the reaction in the step S2 at normal temperature, wherein the mixing time is 150 minutes, and then freezing the digested plasma;
s4, serum preparation: balancing the plasma in the step S3 to room temperature again, centrifuging the plasma by a centrifuge, and filtering the centrifuged solution to prepare animal serum;
s5, serum regulation: adjusting the pH of the serum in the step S4 to 7.5-8.0, adding a certain amount of alum, and standing for 50-150 minutes;
s6, filtering serum: the serum in the step S5 is injected into the cleaning assembly through the sealing frame, and gradually flows downwards from the inside of the filtering frame under the action of certain pressure, so that viruses in the serum can be effectively cleaned through the filtering frame in the cleaning mechanism, different amounts of the filtering frame can be adjusted according to use requirements, and the filtering effect of the serum is ensured;
s7, collecting and packaging: and (3) bottling and collecting the filtered serum in the step (S6), and sealing and packaging the collected serum solution.
The beneficial effects of the invention are as follows:
1. the cleaning device for the exogenous viruses of the animal serum provided by the invention has the advantages that the cleaning component can effectively filter and remove the viruses in the serum, the filtering effect is good, the cleaning is complete, and the use safety of serum biological products is ensured.
2. According to the cleaning device for the exogenous viruses in the animal serum, the locking mechanism can effectively lock and fix the positioning frame, so that the positioning frame is convenient to install and disassemble, the positioning frame can be increased or decreased according to the use requirement of the filtering membrane, and the sufficient filtration of the viruses in the serum is ensured.
3. The invention provides a cleaning device for foreign viruses in animal serum, wherein a filter frame is designed by adopting a sandwich mode, and when a filter membrane is stressed. The bearing plate can protect the filter membrane effectively, prevents the filter membrane from being deformed due to compression, avoids the filter membrane from being damaged, and simultaneously can avoid the change of the diameter of the filter hole on the filter membrane, thereby ensuring the filtering and cleaning effects of serum.
Drawings
The invention will be further described with reference to the drawings and examples.
FIG. 1 is a schematic perspective view of the present invention;
FIG. 2 is a schematic perspective view of the present invention;
FIG. 3 is a schematic cross-sectional view of the structure of FIG. 2 in accordance with the present invention;
FIG. 4 is a schematic perspective view of a cleaning assembly of the present invention;
FIG. 5 is a schematic view of the interior of the cleaning assembly of the present invention;
FIG. 6 is a schematic cross-sectional view of the invention between the ledge, support column and cleaning assembly;
FIG. 7 is a schematic cross-sectional view of the positioning rack and locking mechanism of the present invention;
FIG. 8 is a schematic view of a first cross-sectional configuration of the cleaning mechanism of the present invention;
FIG. 9 is a schematic view of a second cross-sectional configuration of the cleaning mechanism of the present invention;
FIG. 10 is an enlarged schematic view of the structure of FIG. 8A in accordance with the present invention;
fig. 11 is a flow chart of the operational steps of the present invention.
Detailed Description
In order to make the technical problems, technical schemes and beneficial effects to be solved more clear, the invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
As shown in fig. 1 to 11, a cleaning device for foreign viruses in animal serum comprises a baseplate frame 1, a supporting column 2, a base frame 3, a cleaning component 4 and a sealing frame 5, and is characterized in that: the support column 2 is evenly installed to bottom plate frame 1 upper end, installs the base frame 3 between the support column 2 upper end, and clearance subassembly 4 is installed through the screw to base frame 3 upper end, and seal frame 5 is installed through the screw to clearance subassembly 4 upper end.
The base frame 3 be rectangular structure, base frame 3 is connected with clearance subassembly 4 through the screw, and the fluid-discharge tube is installed to base frame 3 lower extreme, and concrete during operation, base frame 3 and clearance subassembly 4 sealing connection prevent to take place the phenomenon of leaking, the fluid-discharge tube can collect the serum after the virus is clear away.
The sealing frame 5 upper end be arc structure, sealing frame 5 is connected with clearance subassembly 4 through the screw, sealing frame 5 upper end is provided with the feed liquor pipe, is provided with the manometer on the feed liquor pipe, and concrete during operation, sealing frame 5 and clearance subassembly 4 sealing connection prevent to take place the phenomenon of leaking, the manometer can accurately show pressure, prevents that the pressure is too big to lead to the phenomenon that the diaphragm takes place deformation.
The cleaning component 4 include casing frame 41, link 42, mounting bracket 43, locating rack 44, locking mechanism 45 and clearance mechanism 46, casing frame 41 be cylindrical structure, the casing frame 41 outside evenly is provided with the open slot, inside symmetry of casing frame 41 installs link 42, link 42 is cross structure, install mounting bracket 43 between the link 42, all be provided with the spout on mounting bracket 43 and the casing frame 41, install locating rack 44 between the spout, the inside locking mechanism 45 that is provided with of locating rack 44, locking mechanism 45 is fixed with the spout chucking, install clearance mechanism 46 in the open slot, clearance mechanism 46 middle part is connected with locating rack 44, clearance mechanism 46 inboard is hugged closely mutually with mounting bracket 43, specific during operation, the manual work is with animal serum through inside sealing frame 5 pours into casing frame 41, clearance mechanism 46 can clear up the filtration to the inside virus of animal serum, clearance mechanism 46 can be dismantled and be connected, the clearance of the inside diaphragm of clearance mechanism 46 of being convenient for, locating rack 44 can increase and decrease according to the quantity of diaphragm, locking mechanism 45 can fix locating rack 44, the installation and dismantlement of locating rack 44 of being convenient for simultaneously, the clearance mechanism 44 can guarantee the clearance effect of supporting mechanism 46 with the sealed side wall.
The cross-section of mounting bracket 43 be cross structure, the symmetry is provided with sealed the pad on the mounting bracket 43 lateral surface, sealed pad is made for wear-resisting rubber material, during the concrete work, when the filter frame 464 is installed inside the mounting bracket 43, the filter frame 464 inboard can hug closely with sealed the pad, prevents to produce the gap between filter frame 464 and the mounting bracket 43, guarantees the abundant filter effect of serum.
The cross section of locating rack 44 be I shape structure, locating rack 44 mid-mounting has locking mechanism 45, and locating rack 44 outside symmetry is provided with the draw-in groove, evenly installs the sealing strip in the draw-in groove, and the sealing strip can play sealed effect effectively, prevents to appear the gap between locating rack 44 and the filter frame 464.
The locking mechanism 45 include regulation pole 451, briquetting 452, wedge 453, guide bar 454, extension spring 455 and pressure strip 456, locating rack 44 middle part be connected with regulation pole 451 through screw-thread fit's mode, the regulation pole 451 outside is provided with the screw, the briquetting 452 is installed to regulation pole 451 inboard, the inside logical groove that is provided with of locating rack 44, lead to the inslot symmetry and be provided with the guide frame, be connected with guide bar 454 through sliding fit's mode in the guide frame, wedge 453 is installed to the guide bar 454 inboard, wedge 453 hugs closely with briquetting 452, be provided with extension spring 455 on the guide bar 454, the pressure strip 456 is installed in the guide bar 454 outside, during the concrete work, when locating rack 44 installs, the manual work is placed locating rack 44 between the spout of casing frame 41 inside, rotate regulation pole 451 through the spanner, regulation pole 451 drives the inside motion of briquetting 452, briquetting 452 outwards moves through wedge 453, the inside the pressure strip 456 of the pressure strip 456 can be hugged closely with the spout, and then can realize fixed effect to locating rack 44, easy operation is convenient simultaneously, do benefit to the installation and dismantlement of locating rack 44.
The pressure strip 456 be protruding structure, pressure strip 456 cooperatees with the spout, evenly is equipped with the non-slip raised on the pressure strip 456 lateral surface, and during the concrete work, when pressure strip 456 hugs closely with the spout is inside, the non-slip raised can increase friction effectively, prevents that locating rack 44 position from taking place the skew.
The cleaning mechanism 46 include clearance frame 461, handle frame 462, connecting plate 463 and filtration frame 464, clearance frame 461 be arc structure, clearance frame 461 cooperatees with the open slot, evenly be provided with the mounting hole on the clearance frame 461, clearance frame 461 outside mid-mounting has handle frame 462, evenly install connecting plate 463 on the concave surface in clearance frame 461, connecting plate 463 position adjustable, connecting plate 463 mid-mounting has filtration frame 464, during specific work, filtration frame 464 can filter the clearance to the serum, when filtration frame 464 needs to wash the change, the manual work is pull down the screw, drive clearance frame 461 outside motion through handle frame 462, connecting plate 463 can fix filtration frame 464, simultaneously connecting plate 463 can carry out position adjustment according to the use needs, the accurate installation of filtration frame 464 of being convenient for.
The filter frame 464 include carrier plate 4641 and filter membrane 4642, carrier plate 4641 quantity is two, installs filter membrane 4642 between the carrier plate 4641, is connected through the screw between two carrier plates 4641 for be the intermediate layer formula design between carrier plate 4641 and the filter membrane 4642, the during specific work, carrier plate 4641 can carry out the bearing to filter membrane 4642 effectively, when preventing that pressure is too big, the phenomenon of deformation takes place for filter membrane 4642, leads to the filter aperture of filter membrane 4642 to change, influences the filter effect of serum.
The supporting plate 4641 is in a fan-shaped structure, arc-shaped grooves are uniformly formed in the outer side face of the supporting plate 4641, and round holes are uniformly formed in the arc-shaped grooves.
The filter membrane 4642 is a bio-permeable membrane, the outer side of the filter membrane 4642 is tightly attached to the inner side of the support plate 4641, and the support plate 4641 can effectively prevent the deformation of the filter membrane 4642 and prevent the deformation of the filter membrane 4642 due to stress.
The filter membrane 4642 is composed of a prefilter microporous membrane and a virus-removing microporous membrane compounded on the surface of the prefilter microporous membrane, wherein the prefilter microporous membrane is made of porous fiber materials and polyvinylidene fluoride, the porous fiber materials are selected from glass fibers, polypropylene fibers or high polymer fibers, and the virus-removing microporous membrane is made of polyvinylidene fluoride or polytetrafluoroethylene.
In addition, the invention also provides a cleaning method of the cleaning device for the exogenous viruses of the animal serum, which specifically comprises the following steps:
s1, filtering plasma: balancing the frozen animal blood plasma to room temperature, filtering the blood plasma by medical gauze, and collecting the filtered filtrate;
s2, adding thrombin: uniformly mixing thrombin solution and calcium chloride solution according to a certain proportion for 28-32 minutes, and then adding the mixed solution into the filtrate in the step S1;
s3, mixing and digestion: carrying out mixed digestion on the plasma after the reaction in the step S2 at normal temperature, wherein the mixing time is 150 minutes, and then freezing the digested plasma;
s4, serum preparation: balancing the plasma in the step S3 to room temperature again, centrifuging the plasma by a centrifuge, and filtering the centrifuged solution to prepare animal serum;
s5, serum regulation: adjusting the pH of the serum in the step S4 to 7.5-8.0, adding a certain amount of alum, and standing for 50-150 minutes;
s6, filtering serum: the serum in the step S5 is injected into the cleaning assembly 4 through the sealing frame 5, and gradually flows downwards from the inside of the filtering frame 464 under the action of certain pressure, the filtering frame 464 in the cleaning mechanism 46 can effectively clean viruses in the serum, and the filtering frame 464 can adjust different amounts according to use requirements, so that the filtering effect of the serum is ensured;
s7, collecting and packaging: and (3) bottling and collecting the filtered serum in the step (S6), and sealing and packaging the collected serum solution.
The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the foregoing embodiments, and that the foregoing embodiments and description are merely illustrative of the principles of this invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, and these changes and modifications fall within the scope of the invention as hereinafter claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. The utility model provides a cleaning device of animal serum exogenous virus, includes underframe (1), support column (2), base frame (3), clearance subassembly (4) and seal frame (5), its characterized in that: the upper end of the bottom plate frame (1) is uniformly provided with support columns (2), a base frame (3) is arranged between the upper ends of the support columns (2), the upper end of the base frame (3) is provided with a cleaning component (4) through a screw, and the upper end of the cleaning component (4) is provided with a sealing frame (5) through a screw, wherein the sealing frame is provided with a sealing groove;
the cleaning assembly (4) comprises a shell frame (41), a connecting frame (42), a mounting frame (43), a positioning frame (44), a locking mechanism (45) and a cleaning mechanism (46), wherein the shell frame (41) is of a cylindrical structure, open slots are uniformly formed in the outer side of the shell frame (41), the connecting frame (42) is symmetrically arranged in the shell frame (41), the connecting frame (42) is of a cross structure, the mounting frame (43) is arranged between the connecting frames (42), sliding grooves are formed in the mounting frame (43) and the shell frame (41), the positioning frame (44) is arranged between the sliding grooves, the locking mechanism (45) is fixedly clamped with the sliding grooves, the cleaning mechanism (46) is arranged in the open slots, the middle of the cleaning mechanism (46) is connected with the positioning frame (44), and the inner side of the cleaning mechanism (46) is tightly attached to the mounting frame (43);
the cleaning mechanism (46) comprises a cleaning frame (461), a handle frame (462), a connecting plate (463) and a filtering frame (464), wherein the cleaning frame (461) is of an arc-shaped structure, the cleaning frame (461) is matched with an opening groove, mounting holes are uniformly formed in the cleaning frame (461), the handle frame (462) is mounted in the middle of the outer side of the cleaning frame (461), the connecting plate (463) is uniformly mounted on the inner concave surface of the cleaning frame (461), the position of the connecting plate (463) is adjustable, and the filtering frame (464) is mounted in the middle of the connecting plate (463);
the filter frame (464) comprises bearing plates (4641) and filter membranes (4642), the number of the bearing plates (4641) is two, the filter membranes (4642) are arranged between the bearing plates (4641), and the two bearing plates (4641) are connected through screws.
2. The device for cleaning exogenous viruses in animal serum as claimed in claim 1, wherein: the base frame (3) is of a rectangular structure, the base frame (3) is connected with the cleaning assembly (4) through screws, and a liquid discharge pipe is arranged at the lower end of the base frame (3).
3. The device for cleaning exogenous viruses in animal serum as claimed in claim 1, wherein: the upper end of the sealing frame (5) is of an arc-shaped structure, the sealing frame (5) is connected with the cleaning assembly (4) through screws, the upper end of the sealing frame (5) is provided with a liquid inlet pipe, and a pressure gauge is arranged on the liquid inlet pipe.
4. The device for cleaning exogenous viruses in animal serum as claimed in claim 1, wherein: the cross section of the mounting frame (43) is of a cross-shaped structure, sealing gaskets are symmetrically arranged on the outer side face of the mounting frame (43), and the sealing gaskets are made of wear-resistant rubber materials.
5. The device for cleaning exogenous viruses in animal serum as claimed in claim 1, wherein: the cross section of the locating frame (44) is of an I-shaped structure, a locking mechanism (45) is arranged in the middle of the locating frame (44), clamping grooves are symmetrically formed in the outer side of the locating frame (44), and sealing strips are uniformly arranged in the clamping grooves.
6. The device for cleaning exogenous viruses in animal serum as claimed in claim 1, wherein: locking mechanism (45) including regulation pole (451), briquetting (452), wedge (453), guide bar (454), extension spring (455) and pressure strip (456), locating rack (44) middle part be connected with regulation pole (451) through screw-thread fit's mode, regulation pole (451) outside is provided with the screw, regulation pole (451) inboard is installed briquetting (452), inside being provided with of locating rack (44) leads to the groove, lead to the inslot symmetry and be provided with the guide bar, be connected with guide bar (454) through sliding fit's mode in the guide bar, wedge (453) are installed to guide bar (454) inboard, wedge (453) are hugged closely with briquetting (452), be provided with extension spring (455) on guide bar (454), pressure strip (456) are installed in guide bar (454) outside.
7. The device for cleaning exogenous viruses in animal serum as claimed in claim 6, wherein: the compressing plate (456) is of a convex structure, the compressing plate (456) is matched with the chute, and anti-slip protrusions are uniformly arranged on the outer side face of the compressing plate (456).
8. The device for cleaning exogenous viruses in animal serum as claimed in claim 1, wherein: the bearing plate (4641) is of a fan-shaped structure, arc-shaped grooves are uniformly formed in the outer side face of the bearing plate (4641), and round holes are uniformly formed in the arc-shaped grooves.
9. The device for cleaning exogenous viruses in animal serum as claimed in claim 1, wherein: the filter membrane (4642) is a biological permeable membrane, and the outer side of the filter membrane (4642) is tightly attached to the inner side surface of the supporting plate (4641).
10. The method for cleaning the cleaning device for the exogenous viruses in the serum of animals according to claim 1, wherein the method comprises the following steps: the method also comprises the following steps:
s1, filtering plasma: balancing the frozen animal blood plasma to room temperature, filtering the blood plasma by medical gauze, and collecting the filtered filtrate;
s2, adding thrombin: uniformly mixing thrombin solution and calcium chloride solution according to a certain proportion for 28-32 minutes, and then adding the mixed solution into the filtrate in the step S1;
s3, mixing and digestion: carrying out mixed digestion on the plasma after the reaction in the step S2 at normal temperature, wherein the mixing time is 150 minutes, and then freezing the digested plasma;
s4, serum preparation: balancing the plasma in the step S3 to room temperature again, centrifuging the plasma by a centrifuge, and filtering the centrifuged solution to prepare animal serum;
s5, serum regulation: adjusting the pH of the serum in the step S4 to 7.5-8.0, adding a certain amount of alum, and standing for 50-150 minutes;
s6, filtering serum: the serum in the step S5 is injected into the cleaning assembly (4) through the sealing frame (5), and gradually flows downwards from the inside of the filtering frame (464) under the action of certain pressure, the filtering frame (464) in the cleaning mechanism (46) can effectively clean viruses in the serum, and the filtering frames (464) can adjust different amounts according to use requirements, so that the filtering effect of the serum is ensured;
s7, collecting and packaging: and (3) bottling and collecting the filtered serum in the step (S6), and sealing and packaging the collected serum solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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| AU1811301A (en) * | 1999-12-02 | 2001-06-12 | General Hospital Corporation, The | Methods for removal, purification, and concentration of viruses, and methods of therapy based thereupon |
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| EP0798003A2 (en) * | 1996-03-21 | 1997-10-01 | Bayer Corporation | Use of structured depth filters for virus removal |
| EP1044696A2 (en) * | 1999-04-12 | 2000-10-18 | B. Braun Melsungen Ag | Removal of enveloped viruses from blood, plasma or serum |
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