CN113615465B - Method for promoting conversion of mango young leaves from heterotrophic to autotrophic - Google Patents

Method for promoting conversion of mango young leaves from heterotrophic to autotrophic Download PDF

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CN113615465B
CN113615465B CN202110813427.3A CN202110813427A CN113615465B CN 113615465 B CN113615465 B CN 113615465B CN 202110813427 A CN202110813427 A CN 202110813427A CN 113615465 B CN113615465 B CN 113615465B
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mango
chlorophyll
fertilizer
magnesium sulfate
iron
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CN113615465A (en
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彭磊
王文涛
周玲
付丽俊
吕鹏悦
黄震
卢辰
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Yunnan Plateau Characteristic Agricultural Industry Research Institute
Yunnan Agricultural University
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Yunnan Plateau Characteristic Agricultural Industry Research Institute
Yunnan Agricultural University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilising, sowing or planting
    • A01C21/005Following a specific plan, e.g. pattern

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Soil Sciences (AREA)
  • Botany (AREA)
  • Fertilizers (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to a method for promoting mango young She Yiyang to be converted into autotrophic, which comprises the steps of planting mango endosperm into a seedling bag, culturing for 20 days, and selecting seedling with uniform growth vigor as a test material; 0-1.5 g of urea with 46% of nitrogen, 0.25-0.75 g of superphosphate with 7.0% of water-soluble phosphorus and 8.0% of available phosphorus, 0.2-0.6 g of magnesium sulfate with 99% of magnesium sulfate and 0.1-0.5 g of ferric ethylenediamine o-dihydroxyacetate with 6% of iron are selected for fertilizer application. The method can remarkably improve the contents of chlorophyll, starch and total sugar in the young mango leaves, the chlorophyll lifting amount is 41%, the contents of starch and total sugar are respectively improved to 46% and 33%, good effects are obtained, and the young mango leaves are promoted to be transformed from heterotrophic into autotrophic.

Description

Method for promoting conversion of mango young leaves from heterotrophic to autotrophic
Technical Field
The invention relates to the technical field of plant cultivation, in particular to a method for promoting mango young She Yiyang to be transformed into autotrophic by fertilization.
Background
The change of mango leaf color can be divided into three stages of a bronze stage, a light green leaf stage and a green leaf stage, wherein a new leaf is initially the bronze stage, the leaf color is purple red, then the new leaf enters the light green leaf stage, at the moment, the leaf color is green but light, and finally the new leaf enters the green leaf stage. The young mango leaves have low chlorophyll content and weak photosynthetic capacity, can not provide nutrition for growth and are in a heterotrophic state. As the leaf matures, the pigment content, morphological structure and the like of the leaf change, the chlorophyll content increases gradually, and the photosynthetic capacity also increases.
Mineral elements are necessary for growth in the growth process of plants, and play an important role in the growth process of plants, and excessive or insufficient mineral elements can influence the normal growth of plants. Mineral elements can be divided into major elements and trace elements according to plant growth demand, wherein the major elements are carbon (C), hydrogen (H), oxygen (O), nitrogen (N), phosphorus (P), potassium (K) and the like, and the trace elements are iron (Fe), magnesium (Mg), zinc (Zn) and the like. Nitrogen, phosphorus and potassium are considered as three elements in the plant growth process and form the basis of plant vital activities. Iron and magnesium are also essential trace elements for plants, wherein iron is the first place of essential trace elements for plants, and can have a great influence on chlorophyll synthesis.
Current technical research:
1. the research on the relation of the chlorophyll content, the specific leaf weight and the photosynthetic rate of the mango leaves shows that different leaf ages have influence on the chlorophyll content and the specific leaf weight of the mango leaves on the photosynthetic rate of the mango leaves and are in very obvious positive correlation.
2. The photosynthetic characteristics of the young leaves, the fully-developed young leaves and the mature leaves of the ginkgo are researched, and the result shows that the photosynthetic capacity of the mature leaves is strongest, the fully-developed young leaves are She Ci, and the young leaves are smallest.
3. The research results of the influence of the mixed application of different nitrogen, phosphorus and potassium fertilizers on the chlorophyll content of the Ficus simplicissima lour show that after the mixed application, the chlorophyll content of the Ficus simplicissima lour can be remarkably improved, the influence effect of the nitrogen fertilizer is maximum, the phosphorus fertilizer is secondary, and the potassium fertilizer is minimum.
4. As a result of applying nitrogen, phosphorus and potassium organic fertilizers with different proportions to potatoes, it is found that the best chlorophyll content is improved by applying 235.5-251.3 kg/hm2 nitrogen, 121.9-123.6-kg/hm 2 phosphorus and Shi Jia 174.5.5-190.2-kg/hm 2 organic fertilizers 37-38 kg/hm 2.
5. The effect study of different fertilization treatments on the chlorophyll content of the leaf of the two-year-old Nanguo pear shows that the chlorophyll content can be increased along with the increase of the amount of nitrogenous fertilizer and phosphate fertilizer, and the chlorophyll content is in a trend of rising and then falling along with the increase of the amount of potash fertilizer.
6. The annual seedling sowing of the red oaks in North America is taken as a test object, and the treatment results of different fertilizers and fertilizing amounts show that the different fertilizing treatments improve the chlorophyll content of the red oaks in North America to a certain extent, and in a certain range, along with the increase of the fertilizer application amount, the chlorophyll content is correspondingly increased, and the influence of the nitrogenous fertilizer and the compound fertilizer on the total chlorophyll content reaches an extremely remarkable level.
7. The short-branch Fuji and Gala apple trees are used as test materials, shi Jia 0.5.5 kg of fixed values are adopted, and nitrogen fertilizer, phosphate fertilizer and organic fertilizer are adopted to carry out 3-factor 3-level secondary regression orthogonal test design, so that research results show that the application amounts of nitrogen, phosphorus and organic fertilizer are respectively 0.5 kg/plant, 0.4 kg/plant and 2.5 kg/plant, the chlorophyll content of mature-stage leaves is the highest, and the senescence of mature-stage leaves can be delayed to a certain extent.
8. The method takes 1-year-old Alnus trabeculosa seedlings as research materials, and adopts orthogonal combined fertilization tests of applying different amounts of nitrogenous fertilizer, phosphate fertilizer and potash fertilizer to determine the total chlorophyll content, and results show that different fertilizer proportions have different effects on the total chlorophyll content of Alnus trabeculosa seedlings, and the ratio of the nitrogen, phosphorus and potassium fertilizer which is most suitable for increasing the chlorophyll content is 0-0.2:0.6:0.1-0.4.
9. The optimal fertilization formula for improving the leaf green content of the camellia oleifera in the forest is urea 1%, dipotassium hydrogen phosphate 0.44% and potassium chloride 0.27%.
10. The leaf of ficus microcarpa is taken as a research object, the influence of different element fertilization on the chlorophyll of the leaf of ficus microcarpa and the mass fraction of the leaf nitrogen is discussed through different fertilization treatments (single fertilization of N, PK and NPK), and the result shows that the different fertilization treatments can obviously influence the contents of chlorophyll a and chlorophyll b, and the mixed fertilization effect of nitrogen, phosphorus and potassium elements is larger than that of a single effect.
11. Nii shows that after nitrogenous fertilizer is applied to peach, chlorophyll content in leaves of peach is increased, photosynthesis of the leaves is also enhanced, and carbon assimilation rate is also increased along with the increase of nitrogen application amount.
12. The research on spraying the phosphate fertilizer on the leaf surfaces of the 'summer black' grape shows that the spraying of the phosphate fertilizer has obvious effect of promoting the growth of chlorophyll on the leaf blades of the 'summer black' grape.
13. Research on spraying the iron fertilizer on the blueberry leaf surfaces shows that the application of the iron fertilizer has a good effect on improving the chlorophyll b content in the blueberry leaf, so that the photosynthetic pigment content in the leaf is obviously increased.
14. The research on the influence of the magnesium fertilizer on the chlorophyll content of the leaves of the Taiwan green dates shows that the magnesium fertilizer can obviously improve the chlorophyll content of the leaves.
15. A correction test for the mangoes suffering from iron deficiency and green loss shows that the application of ferrous sulfate and amino acid iron solutions can improve the active iron content and the total iron content of the leaves of the mangoes suffering from iron deficiency and green loss to different degrees.
At present, fertilization is not used for promoting the conversion of mango young leaves from heterotrophic to autotrophic literature or other reports, and an orchard using the method is not used in actual production.
Disclosure of Invention
The invention aims to provide a method capable of promoting conversion of mango young leaves from heterotrophic to autotrophic. The method realizes the purpose of inducing the contents of chlorophyll, starch and total sugar in the young mango leaves to be increased by fertilizing, thereby promoting the conversion of the young mango leaves from heterotrophic to autotrophic.
The invention relates to a method for promoting mango young She Yiyang to be converted into autotrophy, which is realized by the following technical scheme:
(1) Planting mango endosperm into a seedling bag, and after 20 days of cultivation, selecting seedling with uniform growth vigor as a test material;
(2) Selecting urea with 46% of nitrogen, 7.0% of water-soluble phosphorus, 8.0% of superphosphate with effective phosphorus, 99% of magnesium sulfate with magnesium sulfate and 6% of ferric ethylenediamine o-dihydroxyacetate;
(3) Weighing 0-1.5 g of urea and 0.25-0.75 g of calcium superphosphate, sequentially dissolving in 50 ml of water, and uniformly and slowly pouring into nutrient soil for planting seedling; weighing 0.2-0.6 g of magnesium sulfate, 0.1-0.5 g of ethylenediamine o-dihydroxyacetic acid iron, respectively dissolving in 100 ml of water, uniformly spraying on the front and back surfaces of the leaf surface, and stopping spraying when the leaf surface is provided with underwater drops;
(4) And the fertilizer is applied once every 5 days, and the total application is carried out three times, wherein each time of the fertilizer application is carried out in the weather condition of evening or early morning and no rain, and the contents of chlorophyll, starch and total sugar are obviously improved by 20 days.
The method of the invention can achieve the following positive effects:
the method can remarkably improve the contents of chlorophyll, starch and total sugar in the young mango leaves, the chlorophyll lifting amount is 41%, the contents of starch and total sugar are respectively improved to 46% and 33%, good effects are obtained, and the young mango leaves are promoted to be transformed from heterotrophic into autotrophic.
Detailed Description
It will be appreciated by those skilled in the art that the following examples are illustrative of the present invention and should not be construed as limiting the scope of the invention. The specific techniques or conditions are not identified in the examples and are performed according to techniques or conditions described in the literature in this field or according to the product specifications. The reagents or apparatus used were conventional products available commercially without the manufacturer's attention.
Example 1:
(1) Planting mango endosperm into a seedling bag, and after 20 days of cultivation, selecting seedling with uniform growth vigor as a test material;
(2) Urea containing 46% r of nitrogen, calcium superphosphate containing 7.0% of water-soluble phosphorus and 8.0% of available phosphorus, magnesium sulfate containing 99% of magnesium sulfate and iron ethylenediamine-iron glyoxylate containing 6% of iron;
(3) Weighing 1g of urea and 0.75g of calcium superphosphate, sequentially dissolving in 50 ml of water, and uniformly and slowly pouring into nutrient soil for planting seedling; weighing 0.2g of magnesium sulfate and 0.1g of ethylenediamine o-dihydroxyacetic acid iron, respectively dissolving in 100 ml of water, uniformly spraying on the front and back surfaces of the leaf surface, and stopping spraying when the leaf surface is dripped under water;
(4) And applying the fertilizer once every 5 days, applying the fertilizer three times, wherein each time of applying the fertilizer is performed in the evening or in the morning and under the rainless weather condition, and measuring the chlorophyll, starch and total sugar content on the 20 th day.
The final chlorophyll content was found to be 3.591mg/g, and the starch and total sugar content was 4.167mg/g and 12.187mg/g, respectively.
Example 2:
(1) Planting mango endosperm into a seedling bag, and after 20 days of cultivation, selecting seedling with uniform growth vigor as a test material;
(2) Selecting calcium superphosphate with 7.0 percent of water-soluble phosphorus and 8.0 percent of available phosphorus, potassium chloride with 60 percent of potassium oxide, magnesium sulfate with 99 percent of magnesium sulfate and ethylenediamine-iron glyoxylate with 6 percent of iron;
(3) Weighing 0.25g of calcium superphosphate and 1g of potassium chloride, sequentially dissolving in 50 ml of water, and uniformly and slowly pouring into nutrient soil for planting seedling; weighing 0.2g of magnesium sulfate and 0.5g of ethylenediamine o-dihydroxyacetic acid iron into 100 ml of water respectively, uniformly spraying on the front and back surfaces of the leaf surface, and stopping spraying when the leaf surface is dripped under water;
(4) The fertilizer is applied once every 5 days, and the total application is three times, and each time of the fertilizer is carried out under the weather conditions of evening or early morning and no rain. On day 20, chlorophyll, starch and total sugar content were determined.
Finally, the chlorophyll content was 3.870mg/g, the starch and total sugar content were 4.317mg/g and 13.514mg/g, respectively, and compared with example 1, the chlorophyll, starch and total sugar content were increased by 8%, 4% and 11%, respectively.
Example 3:
(1) Planting mango endosperm into a seedling bag, and after 20 days of cultivation, selecting seedling with uniform growth vigor as a test material;
(2) Selecting calcium superphosphate with 7.0 percent of water-soluble phosphorus and 8.0 percent of available phosphorus, potassium chloride with 60 percent of potassium oxide, magnesium sulfate with 99 percent of magnesium sulfate and ethylenediamine-iron glyoxylate with 6 percent of iron;
(3) Weighing 0.75g of calcium superphosphate and 0.5g of potassium chloride, sequentially dissolving in 50 ml of water, and uniformly and slowly pouring into nutrient soil for planting seedling; weighing 0.6g of magnesium sulfate and 0.3g of ethylenediamine o-dihydroxyacetic acid iron, respectively dissolving in 100 ml of water, uniformly spraying on the front and back surfaces of the leaf surface, and stopping spraying when the leaf surface is dripped under water;
(4) The fertilizer is applied once every 5 days, and the total application is three times, and each time of the fertilizer is carried out under the weather conditions of evening or early morning and no rain. On day 20, chlorophyll, starch and total sugar content were determined;
the final measured chlorophyll content was 4.189mg/g, starch and total sugar content was 4.739mg/g, 12.842mg/g, respectively, and chlorophyll, starch and total sugar content were increased by 17%, 14% and 5% respectively, as compared to example 1.
Example 4:
(1) Planting mango endosperm into a seedling bag, and after 20 days of cultivation, selecting seedling with uniform growth vigor as a test material;
(2) Selecting urea with 46% of nitrogen, 7.0% of water-soluble phosphorus, 8.0% of superphosphate with effective phosphorus, 99% of magnesium sulfate with magnesium sulfate and 6% of ferric ethylenediamine-o-dihydroxyacetate;
(3) Weighing 1.5g of urea and 0.5g of calcium superphosphate, sequentially dissolving in 50 ml of water, and uniformly and slowly pouring into nutrient soil for planting seedling; weighing 0.4g of magnesium sulfate and 0.1g of ethylenediamine o-dihydroxyacetic acid iron fertilizer, respectively dissolving in 100 ml of water, uniformly spraying on the front and back surfaces of the leaf surface, and stopping spraying when the leaf surface is dripped under water;
(4) The fertilizer is applied once every 5 days, and the total application is three times, and each time of the fertilizer is carried out under the weather conditions of evening or early morning and no rain. On day 20, chlorophyll, starch and total sugar content were determined;
the final chlorophyll content was 5.064mg/g, the starch and total sugar content was 6.065mg/g, 16.232mg/g, respectively, and the chlorophyll, starch and total sugar content were increased by 41%, 46% and 33%, respectively, as compared to example 1.
In the above examples, different results were obtained by applying different amounts of fertilizer, the chlorophyll, starch and total sugar contents of example 4 were highest, the chlorophyll content was 5.064mg/g, the starch and total sugar contents were 6.065mg/g and 16.232mg/g, respectively, the chlorophyll lifting amount was 41%, and the starch and total sugar contents were 46% and 33% respectively, giving good results. The formula is proved to promote chlorophyll synthesis, so that the contents of photosynthetic product starch and total sugar are improved, and the conversion of mango young leaves from heterotrophic state to autotrophic state is quickened.
The foregoing has shown and described the basic principles, principal features and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined in the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims (1)

1. A method for promoting the conversion of mango young She Yiyang into autotrophy, which is characterized by comprising the following steps:
(1) Planting mango endosperm into a seedling bag, and after 20 days of cultivation, selecting seedling with uniform growth vigor as a test material;
(2) Selecting urea with 46% of nitrogen, calcium superphosphate with 7.0% of water-soluble phosphorus and 8.0% of available phosphorus, magnesium sulfate with 99% of magnesium sulfate and ethylenediamine-iron glyoxylate with 6% of iron;
(3) Weighing 0-1.5 g of urea and 0.25-0.75 g of calcium superphosphate, sequentially dissolving in 50 ml of water, and uniformly and slowly pouring into nutrient soil for planting seedling; weighing 0.2-0.6 g of magnesium sulfate, 0.1-0.5 g of ethylenediamine o-dihydroxyacetic acid iron, respectively dissolving in 100 ml of water, uniformly spraying on the front and back surfaces of the leaf surface, and stopping spraying when the leaf surface is provided with underwater drops;
(4) And the fertilizer is applied once every 5 days, and the total application is carried out three times, wherein each time of the fertilizer application is carried out in the weather condition of evening or early morning and no rain, and the contents of chlorophyll, starch and total sugar are obviously improved by 20 days.
CN202110813427.3A 2021-07-19 2021-07-19 Method for promoting conversion of mango young leaves from heterotrophic to autotrophic Active CN113615465B (en)

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CN106866247A (en) * 2017-03-09 2017-06-20 四川康沃本原生物科技有限公司 A kind of mango special organic and inorganic fertilizer
CN107746338A (en) * 2017-11-24 2018-03-02 谢育枝 A kind of general fertilizer of mango
CN108440052A (en) * 2018-04-02 2018-08-24 云南省热带作物科学研究所 A kind of fertilizer and preparation, method of administration of biocontrol of mango seedling iron deficiency disease disease
CN112225594A (en) * 2020-09-18 2021-01-15 海南大学 Mango stage nutrition formula and formula using method

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