CN113604490B - 猕猴桃溃疡病感病基因AcBXL1及其应用 - Google Patents
猕猴桃溃疡病感病基因AcBXL1及其应用 Download PDFInfo
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Abstract
本发明公开了猕猴桃溃疡病感病基因AcBXL1及其应用。基因AcBXL1及其编码蛋白的序列分别如SEQ ID NO:1和2所示。本发明通过对猕猴桃基因组和转录组分析，成功获得猕猴桃AcBXL1基因序列，通过瞬时表达和稳转的遗传转化实验，证实基因AcBXL1具有促进猕猴桃对溃疡病的感病性，为后续基因编辑创造抗病新品种及溃疡病的防治工作奠定基础。
Description
技术领域
本发明涉及植物基因工程领域，具体地说，涉及猕猴桃溃疡病感病基因AcBXL1 及其应用。
背景技术
猕猴桃属猕猴桃科猕猴桃属，是上世纪驯化最为成功的水果之一，其富含膳食 纤维和维生素C，营养丰富，经济价值高。我国是猕猴桃属植物的起源地和分布中心， 栽培面积居世界之首，在我国农业供给侧改革、精准扶贫、乡村振兴及生态保护中 发挥重要作用。然而，由丁香假单胞杆菌猕猴桃致病变种(Pseudomonas syringae pv. actinidiae，Psa)引起的猕猴桃溃疡病已在全国猕猴桃产区扩散蔓延，成为影响猕猴 桃产业发展的“卡脖子”问题。
溃疡病主要危害猕猴桃属中华猕猴桃和美味猕猴桃，但品种之间抗病性差异显著。新西兰利用有性杂交，培育出黄肉型耐溃疡品种‘Zesy002’(阳光金果 G3)代替易感溃疡品种‘Hort16A’取得了巨大成功，在有效控制溃疡病的同时，经 济价值也大幅提升，迅速占领了全球猕猴桃高端市场。可见，抗病种质资源的挖掘 与创制是解决猕猴桃溃疡病问题的关键，研究猕猴桃抗溃疡病的调控机制是一个重 大的科学问题。我国现有栽培品种达180种，但是整体上主栽品种对猕猴桃溃疡病较 为敏感。猕猴桃遗传高度杂合，前人对猕猴桃如何调控抗病的分子机理缺乏***了 解。因此，借鉴模式作物的研究结果，挖掘猕猴桃应答溃疡病的重要基因及调控元 件，构建分子网络，提升猕猴桃抗溃疡病的认知水平，为保障我国猕猴桃产业健康 稳定发展奠定理论、技术和材料基础。
猕猴桃溃疡病菌可以长时间附生于猕猴桃叶片、花蕾等表面，通过自然孔口、 伤口等侵入并快速繁殖，随后进入茎叶等维管组织***(Gao et al.2016；Donati et al.2018)。植物细胞壁是抵御大多数病原菌侵染的第一道动态结构屏障(Qiao and Dixon2014；et al.2018)，其主要成分包括纤维素、果胶、半纤维素、木质素和 结构蛋白等。在病原菌诱导下，植物会发生细胞壁木质素沉积(Zhao and Dixon 2011； Hu etal.2018)、胼胝质积累(Luna et al.2010)、活性氧迸发、结构改变(Vorwerk et al.2004)和植保素合成等(Ahuja et al.2012)。同时，细菌会分泌酶和毒素等来 克服、抑制或干扰植物细胞壁介导的抗病性以建立侵染关系。营腐生病原细菌，如 软腐病菌(Erwiniaspp.)，产生各种细胞壁降解酶，分解植物细胞壁，建立侵染关 系(Toth and Birch 2005)；营活体或半活体寄生病原细菌利用一些效应蛋白来抑制 细胞壁介导的防卫反应，如在丁香假单胞菌(P.syringae)中非常保守的效应蛋白CEL 可以抑制水杨酸(SA)介导的细胞壁抗性(DebRoy et al.2004)。
以链霉素、四环素和春雷霉素等抗生素类，硫酸铜等铜制剂类和噻霉酮是目前 猕猴桃溃疡病最有效的防治药剂，但因抗药性及病菌可侵入猕猴桃枝干木质部等问 题造成一旦侵染，药剂很难防控(Colombi et al.2017)。因此，亟需开发新的植物溃疡 病防治方法。
发明内容
本发明的目的是提供猕猴桃溃疡病感病基因AcBXL1及其应用。
为了实现本发明目的，第一方面，本发明提供猕猴桃溃疡病感病基因AcBXL1， 其为编码如下蛋白质(a)或(b)的基因：
(a)由SEQ ID NO:2所示的氨基酸序列组成的蛋白质；或
(b)SEQ ID NO:2所示序列经取代、缺失或添加一个或几个氨基酸且具有同等 功能的由(a)衍生的蛋白质。
基因AcBXL1是从猕猴桃品种‘金魁’中克隆获得的，其核苷酸序列如SEQ ID NO:1所示。
启动子GUS染色结果显示，基因AcBXL1主要表达于猕猴桃的根、茎中，而这些 部位正好是溃疡病主要危害部位。
第二方面，本发明提供含有所述基因AcBXL1的生物材料，所述生物材料包括但 不限于重组DNA、表达盒、转座子、质粒载体、病毒载体、工程菌或非可再生的植 物部分。
第三方面，本发明提供所述基因AcBXL1或含有该基因的生物材料在制备转基因植物中的应用。
第四方面，本发明提供所述基因AcBXL1在猕猴桃抗溃疡病育种中的应用。
本发明中，所述溃疡病由丁香假单胞杆菌猕猴桃致病变种(Pseudomonassyringae pv.actinidiae，Psa)导致，优选具有强致病力的丁香假单胞杆菌猕猴桃致病变种致病 类型3(biovar 3)。
第五方面，本发明提供易感溃疡病的转基因植物的制备方法，所述方法包括： 在植物中过表达所述基因AcBXL1；
所述过表达的方式选自以下1)～5)，或任选的组合：
1)通过导入具有所述基因的质粒；
2)通过增加植物染色体上所述基因的拷贝数；
3)通过改变植物染色体上所述基因的启动子序列；
4)通过将强启动子与所述基因可操作地连接；
5)通过导入增强子。
所述植物包括但不限于猕猴桃、烟草、番茄。
携带有所述目的基因的表达载体可通过使用Ti质粒、植物病毒载体、直接DNA 转化、微注射、电穿孔等常规生物技术方法导入植物细胞中(Weissbach，1998， Method forPlant Molecular Biology VIII，Academy Press，New York，第411-463页； Geiserson和Corey，1998，Plant Molecular Biology，2nd Edition)。
第六方面，本发明提供易感溃疡病的猕猴桃的制备方法，所述方法包括：将所 述基因AcBXL1构建到植物表达载体上，所得重组表达载体通过农杆菌介导法转化猕 猴桃。
优选地，所述植物表达载体为pCAMBIA1300。
优选地，基因AcBXL1受35S启动子驱动表达。
第七方面，本发明提供用于扩增所述基因AcBXL1的PCR引物，包括序列如SEQ IDNO:3所示的上游引物和如SEQ ID NO:4所示的下游引物。
第八方面，本发明提供猕猴桃溃疡病感病基因AcBXL1启动子，其序列为：
i)SEQ ID NO:5所示的核苷酸序列；或
ii)SEQ ID NO:5所示的核苷酸序列经取代、缺失和/或增加一个或多个核苷酸且具有相同功能的核苷酸序列；或
iii)在严格条件下与SEQ ID NO:5所示序列杂交且具有相同功能的核苷酸序列，所述严格条件为在含0.1％SDS的0.1×SSPE或含0.1％SDS的0.1×SSC溶液中，在65℃ 下杂交，并用该溶液洗膜；或
iv)与i)、ii)或iii)的核苷酸序列具有90％以上同源性且具有相同功能的核苷酸序列。
第九方面，本发明提供所述启动子在调控下游基因表达中的应用，所述下游基 因包括但不限于基因AcBXL1，报告基因GFP。
本发明通过对猕猴桃基因组和转录组分析，成功获得猕猴桃AcBXL1基因序列， 通过瞬时表达和稳转的遗传转化实验，证实基因AcBXL1具有对溃疡病的感病性，其 对猕猴桃溃疡病病菌发病具有显著促进作用，为猕猴桃抗病育种提供新思路，为后 续植物溃疡病的防治工作奠定基础。
附图说明
图1为本发明较佳实施例中猕猴桃组培叶片接种溃疡病后的AcBXL1基因的表达分析。其中，左图和右图分别对应抗病品种‘金魁’和感病品种‘红阳’。CK：未 处理的猕猴桃组培苗叶片；CL：Psa处理1、6、12、24、48、96h的猕猴桃组培苗叶 片。
图2为本发明较佳实施例中AcBXL1基因猕猴桃叶片瞬时表型。其中，CK：猕猴 桃叶片注射无菌水；Psa：猕猴桃叶片注射Psa菌液；AcBXL1：猕猴桃叶片注射 p1300-35s-AcBXL1-GFP菌液；Psa+AcBXL1：猕猴桃叶片注射Psa和 p1300-35s-AcBXL1-GFP混合菌液。
图3为本发明较佳实施例中猕猴桃叶片溃疡病的含量。其中，Psa：猕猴桃叶片注射psa菌液；Psa+AcBXL1：猕猴桃叶片注射p1300-35s-AcBXL1-GFP菌液。
图4为本发明较佳实施例中AcBXL1在转基因猕猴桃中的表达情况。
图5为本发明较佳实施例中野生型和AcBXL1转基因猕猴桃叶片接种Psa表型。其中，ck为p1300空载对照，AcBXL1-AcBXL4为p1300-35s-AcBXL1-GFP四个转基因株系。
图6为本发明较佳实施例中AcBXL1基因启动子烟草叶片GUS染色结果。
图7为本发明较佳实施例中Psa诱导AcBXL基因启动子烟草叶片GUS染色结果。
图8为本发明较佳实施例中AcBXL1基因启动子果实GUS染色结果。
图9为本发明较佳实施例中AcBXL1基因启动子稳定转化番茄GUS染色结果。其 中，A为番茄整株植物，B、C、D为番茄的茎、叶、根。
图中，不同小写字母表示差异显著。
具体实施方式
以下实施例用于说明本发明，但不用来限制本发明的范围。若未特别指明，实施例均按照常规实验条件，如Sambrook等分子克隆实验手册(Sambrook J&Russell DW,Molecular Cloning:a Laboratory Manual,2001)，或按照制造厂商说明书建议的条件。
以下实施例中涉及的pCAMBIA1300载体、猕猴桃品种‘金魁’、‘红阳’，以 及其他各种试剂均为市售。
以下实施例中使用的Psa为猕猴桃溃疡病菌，菌株JF8分离自安徽省岳西县主簿镇‘金丰’猕猴桃叶片，MLST研究证实该病菌为丁香假单胞猕猴桃致病变种的致病类 型3，为我国猕猴桃溃疡病的主要致病类型(He et al)，该菌株现保存于中国典型培 养物保藏中心(CCTCC AB2018305)。JF8菌株可参见Rong He#,Pu Liu#,Bing Jia, Shi-zhou Xue,Xiao-jie Wang,Jia-yong Hu,Yosef AI Shoffe,Giorgio M Balestra,Lorenzo Gallipoli,Angelo Mazzaglia,Li-wu Zhu*.Genetic diversity of Pseudomonas syringaepv.actinidiae strains from different geographic regions inChina.Phytopathology.2019, 109：347-357。
实施例1感溃疡病基因AcBXL1的获得及序列分析
1、AcBXL1基因转录组和荧光定量分析
荧光定量实验处理：本试验材料取自抗病猕猴桃品种‘金魁’和感病猕猴桃品种‘红阳’组培苗。用无菌水作对照，用OD600＝0.6的Psa菌液分别处理组培苗1h、6h、12 h、24h、48h、96h后放-80℃冰箱保存样品。
通过对抗病品种‘金魁’和感病品种‘红阳’的转录组分析，挑选出在‘金魁’ 中下调，‘红阳’中上调的基因AcBXL1。通过对‘金魁’和‘红阳’猕猴桃组培叶 片接种Psa菌液1h、6h、12h、24h、48h和96h后，分别对‘红阳’和‘金魁’的AcBXL1 基因的表达量用qRT-PCR技术分析(图1)。‘金魁’猕猴桃组培苗接种Psa后，与对 照相比，接种Psa后AcBXL1基因表达经历了先上调后下调的过程，在6h表达量达到最 大值，而后一直很低。与对照相比，96h后AcBXL1基因的表达量低于对照。‘红阳’ 猕猴桃组培苗接种Psa后，AcBXL1基因的表达量先下调后上调，在48h达到最大值， 最终96h表达量也高于对照。总体来说，AcBXL1基因在抗病品种‘金魁’品种中下调， 在感病品种‘红阳’中上调。这与转录组数据表达趋势一致。
2、基因AcBXL1全长cDNA序列的获得
使用RNA试剂盒提取‘金魁’猕猴桃叶片的RNA。根据基因组结合转录组数据 获取猕猴桃AcBXL1基因CDS完整序列，用Primer 5.0软件设计全长引物。cDNA为模 板，进行PCR扩增，优化后的反应体系如下：
| cDNA | 2.0μl |
| Primer-F(10μM) | 2.0μl |
| Primer-R(10μM) | 2.0μl |
| I-5酶 | 25.0μl |
| ddH2O | 19.0μl |
| 总体积 | 50.0μl |
注：I-5酶购自北京擎科生物科技有限公司。
PCR扩增程序：98℃预变性2min；98℃变性10s，60℃退火20s，72℃延伸1min， 35次循环；72℃再延伸10min，4℃保存。
3、基因AcBXL1序列分析
猕猴桃AcBXL1基因CDS长2202bp(SEQ ID NO:2)，编码734个氨基酸(SEQ ID NO:1)。在Protparam网站分析AcBXL1基因理化性质，缬氨酸(11.6％，85个)在AcBXL1 蛋白中含量相对最多，色氨酸(1.5％，11个)在AcBXL1蛋白中含量相对最少，带正 电荷总残基数(Asp+Glu)共59个。该蛋白预测分子式为C6517H10834N2202O2749S438，相 对分子量为177819.83Da，原子总数为22706，推测其理论半衰期为4.4h，蛋白为不稳 定蛋白。
实施例2感溃疡病基因AcBXL1的遗传转化
1、AcBXL1基因p1300载体的构建(用于基因的瞬时表达和转基因猕猴桃)
根据pCAMBIA1300载体的功能，将目的基因***到35S启动子之后，载体自带 EGFP标记基因，同时满足亚细胞定位和基因超表达的功能。根据p1300载体可供选 择的酶切位点(Xba I和BamH I)，利用诺唯赞提供的软件(CE Design V1.04)设计 出如下同源臂引物：
使用同源加酶切位点的引物对重新PCR扩增目的基因片段，片段纯化并测其浓度。扩增体系如下：
| cDNA | 2.0μl |
| AcBXL1-Xba I-F(10μM) | 2.0μl |
| AcBXL1-BamH I-R(10μM) | 2.0μl |
| I-5酶 | 25.0μl |
| ddH2O | 19.0μl |
| 总体积 | 50.0μl |
同时，对pCAMBIA1300空载大肠杆菌进行活化后摇菌提取质粒，检测浓度，按 照如下体系进行双酶切操作：
| pCAMBIA1300质粒 | 2.0μg |
| Xba I | 2.0μl |
| BamH I | 2.0μl |
| 10×Green Buffer | 4.0μl |
| ddH2O | 补齐至总体积40.0μl |
在水浴锅中37℃酶切1.0h，结束置于65℃、5min使酶失活。1.5％琼脂糖凝胶电 泳进行检测，纯化回收目的片段。
根据诺唯赞提供的软件(CE Design V1.04)计算其片段和载体片段的加入量。 连接体系如下：
| pCAMBIA1300片段 | 200ng |
| 目的基因片段 | 68ng |
| 5×CE II Buffer | 4.0μl |
| Exnase | 2.0ul |
| ddH2O | 补齐至总体积20.0μl |
将混合物置于PCR仪中37℃、30min连接，反应结束后转化到大肠杆菌感受态细 胞Trans1-T1，并进行重组子筛选和测序验证。对经测序验证正确的阳性重组子菌液 中吸取0.5μl在5ml LB(含Kan)液体培养基中37℃、200rpm过夜震荡培养，使用质 粒回收试剂盒提取大肠杆菌中的p1300-35S-JK-AcBXL1-GFP质粒。将重组质粒 p1300-35s-AcBXL1-GFP、转化到GV3101农杆菌中。
培养至48h左右，挑取单菌落置于YEB液体培养基(含Kan和Rif)中28℃、200rpm 摇摇菌检测，摇菌至OD600＝0.6-0.8；对菌液进行检测，引物(1300-F： 5′-CGCTCCTGGACGTAGCCTTCGGGCATGGC-3′和1300-R： 5′-ATTGCGATAAAGGAAAGGCCATCGTTGAA-3′)，pCAMBIA1300空载片段大小 540bp。扩增检测程序：94℃预变性3min；94℃变性30s，62℃退火30s，72℃延伸 3min，35次循环；72℃再延伸10min。检测体系如下：
| 模板菌液 | 1.0μl |
| 1300-F(10μM) | 1.0μl |
| 1300-R(10μM) | 1.0μl |
| rTaq酶 | 10.0μl |
| ddH2O | 7.0μl |
| 总体积 | 20.0μl |
2、猕猴桃的瞬时表达(基因功能验证)
取生长45天、长势良好、叶片大小相同的‘红阳’猕猴桃实生苗，一组注射Psa的 重悬液(OD600＝0.6)，另一组注射p1300-35s-AcBXL1-GFP和Psa按1:1比例混合的重 悬液(OD600＝0.6)；将处理后的猕猴桃实生苗放入25℃光照培养箱，每天观察猕猴 桃发病情况。2周后，用尼康D7500相机对猕猴桃叶片的发病情况进行拍照，并采用 稀释涂布平板法计算猕猴桃溃疡病发病程度。
通过瞬时注射猕猴桃实生苗的整片叶子，25℃培养箱正常培养2周，猕猴桃叶片Psa溃疡病的发病情况如图2所示；通过稀释涂板法，由显微镜观察Psa菌落个数来代 表猕猴桃溃疡病发病程度，计数结果如图3所示。注射过p1300-35s-AcBXL1-GFP与Psa 混合菌液的猕猴桃叶片，菌落总数是注射过Psa菌液叶片菌落总数的1.25倍。
3、农杆菌介导猕猴桃的遗传转化(p1300-35S-JK-AcBXL1-GFP转基因猕猴桃)
(1)预培养：将猕猴桃组培苗的叶柄切成1cm的小段，叶片切成0.5cm×1cm的 小叶盘，叶脉向下平铺到MS+3mg/L ZT+1mg/L NAA的培养基上预培养3-5d。
(2)侵染：将预培养的叶盘与叶柄放到农杆菌转化液里侵染20min；将侵染后 的材料放到无菌滤纸上吸取多余的菌液。
(3)暗培养：之后将材料接种到MS+50μM AS的培养基上28℃暗培养2d；(4) 筛选：将材料用无菌水冲洗3次，吸干水分后接种到MS+3mg/L ZT+1mg/L NAA+ 400mg/L Cef+150mg/L Kan的培养基上培养；筛选过程中，尽量不要更换培养基， 以避免污染。
(5)继代培养：一个月后，将长出的幼苗切出，继续在MS+3mg/L 6-BA+1mg/L NAA+400mg/L Cef+100mg/L Kan的培养基上继代培养。愈伤则继续培养。
(6)诱导生根：将长至3-4cm的猕猴桃幼苗切出，放置在1/2MS+0.7mg/L IBA +50mg/L Kan的培养基上做生根培养。
为确认AcBXL1基因在猕猴桃植株中是否超表达，取同一品种转基因猕猴桃与对照猕猴桃的叶片提取总RNA，进行qRT-PCR扩增，检测AcBXL1基因的相对表达量， 荧光定量PCR结果显示，超表达猕猴桃植株中AcBXL1基因的相对表达量显著高于对 照，是对照植株的60倍(图4)。说明此转基因猕猴桃AcBXL1基因确实超表达。
分别用野生型猕猴桃叶片与转基因猕猴桃叶片接种等量、OD600＝0.2的Psa菌液，放25℃培养箱一周后观察发病情况，如图5所示，转基因猕猴桃叶片发病面积明显大 于野生型猕猴桃叶片。说明AcBXL1基因可加速猕猴桃感病。
实施例3 AcBXL1基因启动子活性分析
1、P1391Z启动子载体的构建
首先使用诺唯赞公司的CE Design V1.04软件对测序正确的AcBXL1基启动子序列设计酶切引物(表1)。用克隆出正确序列的AcBXL1基因启动子菌液划板、挑单、摇 AcBXL1启动子菌液，以AcBXL1启动子菌液为模板，用KOD酶进行高保真PCR扩增， PCR反应结束后在PCR管中中加入10μL 6×Loading Buffer吸打混匀，用于后续电泳检 测和并切胶回收。
表1启动子载体构建所需引物
用Takara公司的快切酶quickcut-BamHI和quickcut-EcoRI对pCambia 1391Z载体的 酶切位点BamHI和EcoRI进行双酶切，电泳检测得到目条带后，胶回收线性化载体pCambia 1391Z。
重组反应使用HieffPlus One Step Cloning Kit酶，将***片段AcBXL1启动 子与线性化载体pCambia 1391Z进行重组反应，反应结束后，将重组产物转化到大肠杆菌里，对得到的单菌落用PCR鉴定、送去测序。AcBXL1基因启动子序列如SEQ ID NO:5所示。
2、AcBXL1基因启动子活性烟草瞬时表达(瞬转)
用温室培养的本氏烟草进行p1391-AcBXL1-GUS启动子活性的瞬时表达，使用前两天对烟草控水处理。活化p1391-GUS阴性对照、p1391-AcBXL1-GUS菌液、p1391-35s-GUS阳性对照，在超净台挑取单克隆，置于30mL YEB+Kan+Rif的锥形瓶 中，将锥形瓶放入28℃培养箱过夜震荡培养。用GUS侵染液重悬菌液，5500rmp离心 8min，重复2-3次，调至OD600＝0.6。为了复苏菌液，需要将p1391-AcBXL1-GUS侵染 液放28℃培养箱震荡培养1-2h。选择烟草叶片大小、生长状况一致、叶面平整的叶片， 从叶背面注射p1391-AcBXL1-GUS侵染液，注满整片叶子。试验完成后把烟草植株避 光处理两天，而后进行染色处理。
使用培养6-7周后的本氏烟草叶片进行瞬时表达，将带有GUS表达序列的AcBXL1启动子菌液注射到本氏烟草叶片中，以pC1391z-GUS载体为阴性对照，***35S启动 子的pC139z1-GUS载体为阳性对照，在37℃培养箱暗培养48h后浸泡在GUS染色中。 2天后，用75％的酒精脱色6h，再用90％的酒精脱色48h,直到完全脱去叶片的叶绿素。 图6结果显示，阴性对照pC139z1-GUS经GUS染色液染色后没有蓝色， pC139z1-35s-GUS阳性对照呈现深蓝色，pC1391z-AcBXL1-GUS基因启动子呈蓝色， 说明AcBXL1基因启动子在叶片中具有活性。
3、Psa病菌诱导后启动子烟草叶片的瞬时表达
将Psa菌液与AcBXL1启动子菌液1:1混合后对本氏烟草叶片进行瞬时表达，以pC1391z-GUS载体为阴性对照，***35S启动子的pC139z1-GUS载体为阳性对照，暗 培养48h后浸泡在GUS染色中。图7结果显示，经Psa病菌介导的pC1391z-AcBXL1-GUS 基因启动子染色后呈蓝色，且颜色比pC1391z-AcBXL1-GUS基因启动子颜色更深。
4、AcBXL1基因启动子猕猴桃果实的瞬时表达
将OD600＝0.6的p1391-AcBXL1-GUS的重悬液用注射器注射到猕猴桃果实中，在 37℃培养箱暗培养2天后，将猕猴桃果实浸泡在GUS染色液中染色。2天后，先用75％ 的酒精脱色6h，再用90％的酒精脱色2天。结果见图8，阴性对照pC139z1-GUS经GUS 染色液染色后没有蓝色，pC139z1-35s-GUS阳性对照呈现深蓝色， pC1391z-AcBXL1-GUS基因启动子呈蓝色，说明AcBXL1基因启动子在猕猴桃果实中具 有活性。
5、农杆菌介导番茄的遗传转化(启动子稳转番茄)
野生型番茄(Lycopersicon esculentum nudicaulis)种子放在37℃培养箱用无菌水 浸泡8h后拿出待用。在无菌超净台进行以下所有操作：将种子放入无菌组培瓶中， 用5ml移液枪吸取75％的酒精,浸泡3-5min，给番茄种子表面消毒；将番茄种子移入新 的无菌组培瓶中，用无菌水冲洗番茄种子3-5次，洗去番茄种子表面的酒精；再用15％ 次氯酸钠浸泡番茄种子5-10min，无菌水冲洗3-5次，洗去种子表面的次氯酸钠溶液； 最后把番茄种子放在灭菌的滤纸上，直到吸干种子表面的多余的水分。把种子移入 1/2MS培养基中，用黑布盖上，暗培养3-5天后放在组培室正常培养。1-2周后，番茄 成苗。
待番茄成苗后，将番茄组培苗的叶子和茎切成1cm×1cm的叶盘并且去除生长 点，放到MS+1mg.L-1ZT+1mg.L-1IAA的培养基上，用黑布盖住，暗培养2-3d。2 天后，拿出叶盘，将预培养过的叶片和茎放到番茄侵染液里侵染4-5min，转移到无 菌的滤纸上，吸干多余的农杆菌，放到MS+1mg.L-1ZT+1mg.L-1IAA的培养基上 进行共生培养，用黑布盖住暗培养2天。2天后用无菌水冲洗，洗去番茄叶片和茎上 残留的农杆菌，放在无菌滤纸上，吸去多余的水分后转移到到MS+1mg.L-1ZT+1 mg.L-1IAA+500mg.L-1Cef+3mg.L-1Hyg的培养基上进行筛选培养，每隔20天换一 次培养基，直至长出愈伤组织和芽。最后转移到MS+0.1mg.L-1IAA+250mg.L-1Cef +3mg.L-1Hyg的番茄生根培养基中生长。长出根后移栽到营养土中。
将p1391-AcBXL1-GUS通过农杆菌介导法转基因番茄，得到番茄的转基因的阳性植株。通过对整株植物的GUS染色，图9结果显示，AcBXL1基因启动子在根茎叶中均 表达，在叶片中表达量较低，而在茎和根中表达量较高。
虽然，上文中已经用一般性说明及具体实施方案对本发明作了详尽的描述，但 在本发明基础上，可以对之做一些修改或改进，这对本领域技术人员而言是显而易 见的。因此，在不偏离本发明精神的基础上所做的这些修改或改进，均属于本发明 要求保护的范围。
序列表
<110> 安徽农业大学
<120> 猕猴桃溃疡病感病基因AcBXL1及其应用
<130> KHP211119315.4
<160> 5
<170> SIPOSequenceListing 1.0
<210> 1
<211> 2202
<212> DNA
<213> 猕猴桃(Actinidia chinensis Planch)
<400> 1
atggaagaaa aggtccaaca actagtgaac aacgccaagg gcatttccag actaggcgtg 60
cccgcgtacg agtggtggtc cgaggccctt cacggggtct cgaacaccgg gccaggtgta 120
cacttcaacg ccacggtccc tggtgctact agtttccctg ccgtgattct gtcagcggct 180
agttttaatt cgtcactgtg gtacgagatg ggccgggtgg tgtcgaccga ggctcgagcc 240
atgtacaatg tgggcttggc cgggctcacg ttttggagcc cgaatgtgaa tgtatttcgg 300
gacccgagat ggggcagggg tcaggagacc cctggtgagg acccactggt ggtttctaaa 360
tatgctgtga attatgttag aggcttgcag gaggtgggcc aagaggggaa ttttagtaac 420
gactctaatg acaagcttaa ggtttcaagt tgttgtaagc attatactgc ttatgatgtg 480
gataattgga aaggggttga tcgatttcat tttgacgcaa aggtaagttt acaggacctg 540
gaagatacat atcagccacc attcaagagc tgtgtggagg agggacatgt cagtagtgtg 600
atgtgctcat ataacagggt caatggtatc cctacttgtg ctgacccaaa ccttctcaaa 660
gggattatca gagaccaatg gaatttagat ggatatattg tgtctgactg tgactctgtg 720
gaggtttact acaactccat acattacacc gctacacctg aggatgcagt agcccttgca 780
cttaaagcag gtttaaacat gaattgtggg gcttatctag ggaagtacac acaaaatgca 840
gttaaatcaa ataaagtgga agagtccatt gtagaccagg ccttgatcta caactacata 900
gtcctaatga ggcttggctt ctttgatggt gaccccacca ccctcccctt cggccaactc 960
ggaccgtccg atgtgtgcac cgacgatcac caagcgttgt ctctcgaagc cgcgaagcag 1020
ggcatagttt tgctagaaaa cagaggatcc ctccccctat ccccaaacac caccaaaaat 1080
atagccctca taggacccaa tgccaatgtc acacaagtta tgataagcaa ctatgcgggc 1140
gtaccatgcc ggtacaccac acctttacaa gggctacaaa aatatgtccc aacagtgaca 1200
tatgaggtgg ggtgtgccaa tgtgggatgt ggggatgaga gaggaatcgg gccggcggtc 1260
aaggcggcgg ccacggccga cgccgtggtg gtggtggttg ggctggatca gtccattgag 1320
agggagggat tggatagggt gaacttgaca ttgccagggt ttcaagagag gcttgtgatg 1380
gaagtggcta atgcaacaaa tggaaatgtg attgttgtga taatgtcagc tggtccaatt 1440
gatgtgtcct ttgccaaaaa taatagcaag attggggcaa ttttgtgggt ggggtaccct 1500
ggccaggcag gaggggatgc cattgctcag gtcatatttg gggactataa tccaggtgga 1560
agatcacctt ttacctggta cccacagaag tatgtagatg agctgccaat gacagacatg 1620
accatgagag ctaatgccac tcgaaactat ccggggcgaa cctaccggtt ctacacaggc 1680
gaaccgatat ataaatttgg acacgggcta agttattcga cattttcgaa attcataata 1740
tcagctcaat ccactatact tatacacgca ttgcctcaaa attacacaaa taatattgtt 1800
caaacccaaa tcaatggtca atatgttgac atttctacga ttgactgtca gaatttgcag 1860
ctcgagctgg ttgtaggggt gtggaatggt ggtccgatgg atggggccca tgtagtgatg 1920
gtgttttgga agccggctag ttcatatgtg gtggtaggga tgccgaattt ggagctggtg 1980
gggtttgaga gggtgggggt gcagagaggg aagatggaga cggtgacggt gaagttggat 2040
gtgtgtaaga ggctaagtgt ggtggatgaa gatgggaaga ggaaggtggt cactgggcag 2100
catacccttt tggttgggtc ttctaatgag aggcaagtga aacactatgt caatattagt 2160
gtggctaaaa gtgagggtgt agtaggtaaa ttacccatgt aa 2202
<210> 2
<211> 733
<212> PRT
<213> 猕猴桃(Actinidia chinensis Planch)
<400> 2
Met Glu Glu Lys Val Gln Gln Leu Val Asn Asn Ala Lys Gly Ile Ser
1 5 10 15
Arg Leu Gly Val Pro Ala Tyr Glu Trp Trp Ser Glu Ala Leu His Gly
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Val Ser Asn Thr Gly Pro Gly Val His Phe Asn Ala Thr Val Pro Gly
35 40 45
Ala Thr Ser Phe Pro Ala Val Ile Leu Ser Ala Ala Ser Phe Asn Ser
50 55 60
Ser Leu Trp Tyr Glu Met Gly Arg Val Val Ser Thr Glu Ala Arg Ala
65 70 75 80
Met Tyr Asn Val Gly Leu Ala Gly Leu Thr Phe Trp Ser Pro Asn Val
85 90 95
Asn Val Phe Arg Asp Pro Arg Trp Gly Arg Gly Gln Glu Thr Pro Gly
100 105 110
Glu Asp Pro Leu Val Val Ser Lys Tyr Ala Val Asn Tyr Val Arg Gly
115 120 125
Leu Gln Glu Val Gly Gln Glu Gly Asn Phe Ser Asn Asp Ser Asn Asp
130 135 140
Lys Leu Lys Val Ser Ser Cys Cys Lys His Tyr Thr Ala Tyr Asp Val
145 150 155 160
Asp Asn Trp Lys Gly Val Asp Arg Phe His Phe Asp Ala Lys Val Ser
165 170 175
Leu Gln Asp Leu Glu Asp Thr Tyr Gln Pro Pro Phe Lys Ser Cys Val
180 185 190
Glu Glu Gly His Val Ser Ser Val Met Cys Ser Tyr Asn Arg Val Asn
195 200 205
Gly Ile Pro Thr Cys Ala Asp Pro Asn Leu Leu Lys Gly Ile Ile Arg
210 215 220
Asp Gln Trp Asn Leu Asp Gly Tyr Ile Val Ser Asp Cys Asp Ser Val
225 230 235 240
Glu Val Tyr Tyr Asn Ser Ile His Tyr Thr Ala Thr Pro Glu Asp Ala
245 250 255
Val Ala Leu Ala Leu Lys Ala Gly Leu Asn Met Asn Cys Gly Ala Tyr
260 265 270
Leu Gly Lys Tyr Thr Gln Asn Ala Val Lys Ser Asn Lys Val Glu Glu
275 280 285
Ser Ile Val Asp Gln Ala Leu Ile Tyr Asn Tyr Ile Val Leu Met Arg
290 295 300
Leu Gly Phe Phe Asp Gly Asp Pro Thr Thr Leu Pro Phe Gly Gln Leu
305 310 315 320
Gly Pro Ser Asp Val Cys Thr Asp Asp His Gln Ala Leu Ser Leu Glu
325 330 335
Ala Ala Lys Gln Gly Ile Val Leu Leu Glu Asn Arg Gly Ser Leu Pro
340 345 350
Leu Ser Pro Asn Thr Thr Lys Asn Ile Ala Leu Ile Gly Pro Asn Ala
355 360 365
Asn Val Thr Gln Val Met Ile Ser Asn Tyr Ala Gly Val Pro Cys Arg
370 375 380
Tyr Thr Thr Pro Leu Gln Gly Leu Gln Lys Tyr Val Pro Thr Val Thr
385 390 395 400
Tyr Glu Val Gly Cys Ala Asn Val Gly Cys Gly Asp Glu Arg Gly Ile
405 410 415
Gly Pro Ala Val Lys Ala Ala Ala Thr Ala Asp Ala Val Val Val Val
420 425 430
Val Gly Leu Asp Gln Ser Ile Glu Arg Glu Gly Leu Asp Arg Val Asn
435 440 445
Leu Thr Leu Pro Gly Phe Gln Glu Arg Leu Val Met Glu Val Ala Asn
450 455 460
Ala Thr Asn Gly Asn Val Ile Val Val Ile Met Ser Ala Gly Pro Ile
465 470 475 480
Asp Val Ser Phe Ala Lys Asn Asn Ser Lys Ile Gly Ala Ile Leu Trp
485 490 495
Val Gly Tyr Pro Gly Gln Ala Gly Gly Asp Ala Ile Ala Gln Val Ile
500 505 510
Phe Gly Asp Tyr Asn Pro Gly Gly Arg Ser Pro Phe Thr Trp Tyr Pro
515 520 525
Gln Lys Tyr Val Asp Glu Leu Pro Met Thr Asp Met Thr Met Arg Ala
530 535 540
Asn Ala Thr Arg Asn Tyr Pro Gly Arg Thr Tyr Arg Phe Tyr Thr Gly
545 550 555 560
Glu Pro Ile Tyr Lys Phe Gly His Gly Leu Ser Tyr Ser Thr Phe Ser
565 570 575
Lys Phe Ile Ile Ser Ala Gln Ser Thr Ile Leu Ile His Ala Leu Pro
580 585 590
Gln Asn Tyr Thr Asn Asn Ile Val Gln Thr Gln Ile Asn Gly Gln Tyr
595 600 605
Val Asp Ile Ser Thr Ile Asp Cys Gln Asn Leu Gln Leu Glu Leu Val
610 615 620
Val Gly Val Trp Asn Gly Gly Pro Met Asp Gly Ala His Val Val Met
625 630 635 640
Val Phe Trp Lys Pro Ala Ser Ser Tyr Val Val Val Gly Met Pro Asn
645 650 655
Leu Glu Leu Val Gly Phe Glu Arg Val Gly Val Gln Arg Gly Lys Met
660 665 670
Glu Thr Val Thr Val Lys Leu Asp Val Cys Lys Arg Leu Ser Val Val
675 680 685
Asp Glu Asp Gly Lys Arg Lys Val Val Thr Gly Gln His Thr Leu Leu
690 695 700
Val Gly Ser Ser Asn Glu Arg Gln Val Lys His Tyr Val Asn Ile Ser
705 710 715 720
Val Ala Lys Ser Glu Gly Val Val Gly Lys Leu Pro Met
725 730
<210> 3
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 3
atggaagaaa aggtccaaca 20
<210> 4
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 4
catgggtaat ttacctacta caccc 25
<210> 5
<211> 2135
<212> DNA
<213> 猕猴桃(Actinidia chinensis Planch)
<400> 5
tgccctttag ttgttggacc ttttcttcca aggtcaggcg cgacactagg tccttggccc 60
ggtccaaata cgacaaagtg gtgttgcaaa ataggtagcc tctcgtgttt ggatcatttt 120
tgttacaagc aaattggtga gtggtttgtg ggattgttag gaaaaatgag aaaaaaacag 180
gtaaaagaag gtgtttcatg gaattttatt atttttttat ctttggtaat tttgggtatt 240
gctctctttt tttagctctc aatctggttt tatactacca tgaaatatga gcagaaattc 300
aaaaagtttc tttcaactta ttccttgcac actaaaacag gatgttactt tttttcccac 360
ttgctaaatt gacaaggtta aagttgcaaa gataaagaat tgatcatctc aagcaaggtt 420
tggagagaga gagagagaga gagagagaga gttttgggca agggtatctt tgaagagatt 480
gcaaagatag aacacacatg ccaaggaaca catgccacca aaggcaaggc attgttctct 540
taagaaaaaa gctatcaatt tctgaagaaa ttataggcaa gtttgggcta agttcttttt 600
gagtttcttg ctattttcat tattggattt tttgtcttta gtcaatgttg tttgatcaaa 660
aatttatatt ttattatttt ttttgacgag atatttgaat tttaatagat ttcaattaaa 720
tacttgaact ttgaatttcg tcatcaatta aaccccttaa ctcaaattca attacaaatt 780
ggacagaaca atccatgttg gcacttagtt ggccactcgt ggtgctaact tgaaaaccct 840
aatactttgg atagagagtg actaggaaaa ttagggttct tttacactta attattgtcg 900
aacgaatgtt tataattaga ttgtggattc aatcgaaaat ggtttggcat ttgagaaggc 960
aggattgtgg gcctacaatg cattatggtt ttcaagttaa tatcatgtgt gaccagctaa 1020
gtaccaacat gagttgttcc gtctaatttt ttacagaatt tgagcttaga ggtctaattg 1080
atgatgtatt ttaaagttca tatatattta attaaagttt tttaaagttc aggtatgaac 1140
gagacatttg gttgaaaatt tagttttttt ttttatattt tccctaaaaa aaattagatg 1200
tatcagcaaa acataatcca cacaacttct gttattgcaa gtaaatggaa atagataatt 1260
acagccaaat aaatacaatt ttaagatgaa ttgcctttat ctgattttcc aactcaatcc 1320
caaaaatgaa aatccagaac cctgtatagc aacaaatgat aatctgcttg tatgtttctt 1380
tgcgaatcga gtgaggtttt cacaggagag gaagaaaacc gaagcgagaa gccacggaag 1440
caagtaaccg aagatggttg gcatatgaga aaataaggta gctgcgcccg agcaagtatc 1500
ataacctcaa agaaaataac tggtcactgc gtctcatcat caggtgttta atacatctcg 1560
atggatagtt aagattgttc taaagttgaa agtgagggtt taatatgaat aaattagaaa 1620
tgaagggatt atttgataag agtgtcagac tctagggtct tttttgtaat ttccccaaat 1680
atcaattaag caatttcatt atgaccaaat tcttaaaata tttaaagatt tattttcttt 1740
aatttttttg aaatgtttaa aattatttca atttcttgtt attcaaatgt aagattaata 1800
gtatggggac cacgtttttg caatacgttt gattactaaa tattttattt aatgatcata 1860
taagatatta taccagataa tattatctaa atatgatgca cacattgttc ataatgaaat 1920
tgtcgccaat ttaataataa aactcaatag atattattaa caaatgaaac catttggttc 1980
tatatgatat tagtgggaaa taattgattc agtttacata tttttttgtg tttatgcatt 2040
atgtaaataa attttggggt caaatttaaa taataaattt ttttatgtga ataaaatcga 2100
ataatttaaa gttttgcaaa ctatatttat gattc 2135
Claims (5)
1.易感溃疡病的转基因植物的制备方法，其特征在于，所述方法包括：在植物中过表达猕猴桃溃疡病感病基因AcBXL1；其中，基因AcBXL1为编码由SEQ ID NO:2所示的氨基酸序列组成的蛋白质的基因；
所述过表达的方式选自以下1）~5），或任选的组合：
1）通过导入具有所述基因的质粒；
2）通过增加植物染色体上所述基因的拷贝数；
3）通过改变植物染色体上所述基因的启动子序列；
4）通过将强启动子与所述基因可操作地连接；
5）通过导入增强子；
所述溃疡病由丁香假单胞杆菌猕猴桃致病变种（Pseudomonas syringae pv.actinidiae）导致；
所述植物为猕猴桃。
2.根据权利要求1所述的方法，其特征在于，所述丁香假单胞杆菌猕猴桃致病变种为具有强致病力的丁香假单胞杆菌猕猴桃致病变种致病类型3。
3.易感溃疡病的猕猴桃的制备方法，其特征在于，所述方法包括：将猕猴桃溃疡病感病基因AcBXL1构建到植物表达载体上，所得重组表达载体通过农杆菌介导法转化猕猴桃；其中，基因AcBXL1为编码由SEQ ID NO:2所示的氨基酸序列组成的蛋白质的基因。
4.根据权利要求3所述的方法，其特征在于，所述植物表达载体为pCAMBIA1300。
5.根据权利要求3或4所述的方法，其特征在于，基因AcBXL1受35S启动子驱动表达。
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002022675A2 (en) * | 2000-09-15 | 2002-03-21 | Syngenta Participations Ag | Plant genes, the expression of which are altered by pathogen infection |
| WO2012117406A2 (en) * | 2011-03-02 | 2012-09-07 | Futuragene Israel Ltd. | Bacterial resistant transgenic plants |
| CN111500619A (zh) * | 2019-01-31 | 2020-08-07 | 中国科学院分子植物科学卓越创新中心 | 一种bxl基因或其编码蛋白的应用 |
-
2021
- 2021-08-12 CN CN202110924911.3A patent/CN113604490B/zh active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002022675A2 (en) * | 2000-09-15 | 2002-03-21 | Syngenta Participations Ag | Plant genes, the expression of which are altered by pathogen infection |
| WO2012117406A2 (en) * | 2011-03-02 | 2012-09-07 | Futuragene Israel Ltd. | Bacterial resistant transgenic plants |
| CN111500619A (zh) * | 2019-01-31 | 2020-08-07 | 中国科学院分子植物科学卓越创新中心 | 一种bxl基因或其编码蛋白的应用 |
Non-Patent Citations (3)
| Title |
|---|
| Assessment of 3 Tac-I/Beta, an antimicrobial peptide source, in the control of Pseudomonas plant pathogens;E.A. Moya-Elizondo et al.;《ISHS》;第33-38页 * |
| Pilkington,S. et al..Beta-D-xylosidase [Actinidia chinensis var. chinensis] GenBank: PSS26140.1.《genbank》.2018,第1-2页. * |
| 猕猴桃细菌性溃疡病研究进展;王涛等;《中国农学通报》;第36卷(第3期);第123-128页 * |
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