CN113578170A

CN113578170A - Controllable electromagnetism refiner is used in nucleic acid preparation

Info

Publication number: CN113578170A
Application number: CN202110945136.XA
Authority: CN
Inventors: 刘宗文; 雍金贵; 梁德斌; 刘倩; 李孝才
Original assignee: General Biosystems (anhui) Inc
Current assignee: General Biosystems (anhui) Inc
Priority date: 2021-08-17
Filing date: 2021-08-17
Publication date: 2021-11-02
Anticipated expiration: 2041-08-17
Also published as: CN113578170B

Abstract

The invention relates to a controllable electromagnetic homogenizer for nucleic acid preparation, which belongs to the technical field of nucleic acid preparation equipment and comprises a rack assembly, wherein an adjusting mechanism is arranged on the rack assembly, a clamping mechanism is arranged on one side of the adjusting mechanism, a mounting seat is fixed at the top end of the rack assembly, a transmission mechanism is arranged at the top end of the mounting seat, a stirring mechanism is arranged on the transmission mechanism, and a homogenizing assembly is arranged below the stirring mechanism; a first gear and a second gear are respectively meshed in a gear ring of the stirring mechanism, a connecting frame is arranged below the gear ring, the top end part of the connecting frame is rotatably connected with the two second gears, an auxiliary shaft is rotatably connected to the connecting frame, the top end of the auxiliary shaft is fixedly connected with the first gear, a sealing bowl is fixedly connected to the bottom end of the connecting frame, and a main shaft is fixed in the center of the connecting frame; through rabbling mechanism's setting, realized the homogenate subassembly in the short time with the material shear, polish and decompose, improved the efficiency of homogenate, reduced the nucleic acid extraction time, it is also very simple to manipulate simultaneously.

Description

Controllable electromagnetism refiner is used in nucleic acid preparation

Technical Field

The invention belongs to the technical field of nucleic acid preparation equipment, and particularly relates to a controllable electromagnetic homogenizer for nucleic acid preparation.

Background

In the field of genetic engineering, nucleic acid molecules are the main subject of research, so nucleic acid preparation (i.e., isolation and extraction) is an important step in research. The quality of the nucleic acid sample will be directly related to the success or failure of the experiment. Nucleic acids are divided into deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), the DNA is mainly present in chromosomes of cell nuclei, and there are also a small amount of DNA outside the nuclei, such as mitochondrial DNA (mtdna), chloroplast DNA (cpdna), and plasmid DNA; RNA is present in the cytoplasm, e.g., mRNA, rRNA, tRNA, etc. In addition to the DNA and RNA mentioned above, there are viruses and bacteriophages that exist in a non-cellular form and contain either only DNA or only RNA.

In the process of preparing nucleic acid, the integrity of the primary structure of nucleic acid should be ensured, and the pollution of other nucleic acid molecules should be eliminated, so that the operation steps are simplified as much as possible, the extraction process is shortened, and the degradation of the nucleic acid by physical and chemical factors is reduced. The existing homogenizer for preparing nucleic acid has complex operation and long extraction process, is easy to introduce foreign pollutants in the operation engineering and is not beneficial to the preparation of nucleic acid; therefore, it is necessary to design a homogenizer for nucleic acid preparation which is convenient and fast to operate.

Disclosure of Invention

The invention aims to provide a controllable electromagnetic homogenizer for nucleic acid preparation, which solves the problems of complex operation and long extraction process of the homogenizer in the prior art.

The purpose of the invention can be realized by the following technical scheme:

a controllable electromagnetic homogenizer for nucleic acid preparation comprises a rack assembly, wherein an adjusting mechanism is mounted on the rack assembly, a clamping mechanism is mounted on one side of the adjusting mechanism, a mounting seat is fixed at the top end of the rack assembly, a transmission mechanism is mounted at the top end of the mounting seat, a stirring mechanism is mounted on the transmission mechanism and is positioned right above the clamping mechanism, and a homogenizing assembly is mounted below the stirring mechanism;

the stirring mechanism comprises a gear ring, a first gear and two second gears are respectively meshed in the gear ring, the first gear and the two second gears are oppositely arranged in an equilateral triangle mode, a connecting frame is arranged below the gear ring, the tops of the two ends of the connecting frame are respectively connected with the two second gears in a rotating mode, the other end of the connecting frame is connected with an auxiliary shaft in a rotating mode, the top end of the auxiliary shaft is fixedly connected with the first gear, a groove is formed in the bottom end of the auxiliary shaft, a sealing bowl is fixedly connected to the bottom end of the connecting frame, a main shaft is fixed at the center of the connecting frame, a driven belt wheel is installed at the top end of the main shaft, the auxiliary shaft can rotate around the main shaft, and the auxiliary shaft can also rotate at a high speed.

Further, the rack assembly comprises a base, a sliding column is fixed on the upper surface of the base, two limiting rings are sleeved on the sliding column and fixedly connected with the sliding column, a rack is arranged between the two limiting rings and parallel to the sliding column and fixedly connected with the sliding column.

Further, adjustment mechanism includes the sliding sleeve, the sliding sleeve cup joint on traveller and rack and with traveller and rack sliding connection, be equipped with adjusting gear between sliding sleeve and the rack, adjusting gear meshes with the rack mutually, the fixed pivot in adjusting gear center is rotated with the sliding sleeve and is connected, and the one end of pivot runs through the sliding sleeve and installs the handle, can drive adjusting gear through the handle and rotate, make the sliding sleeve reciprocate along the traveller, the one end that adjusting gear was kept away from to the sliding sleeve is equipped with the support, the one end and the sliding sleeve fixed connection of support.

Furthermore, one side of the sliding sleeve is in threaded connection with a bolt, the threaded end of the bolt penetrates through the sliding sleeve to be abutted to the sliding column, and the sliding sleeve can be well fixed after the height is determined.

Further, clamping mechanism includes the tray, and it has a plurality of spouts to open on the tray, and a plurality of spouts use the tray center to be circular array distribution as the centre of a circle, and sliding connection has the slider in the spout, and the below of tray is equipped with the connection pad, is equipped with the connecting rod between connection pad and the tray, and the both ends of connecting rod articulate with the bottom of slider and week side of connection pad respectively, and the bottom mounting of connection pad has the cylinder, and the output of cylinder runs through the bottom center fixed connection of connection pad and tray, the bottom of tray and the other end fixed connection of support. The connecting disc is driven to move up and down through the air cylinder, the sliding blocks can be driven to slide close to or away from each other in the sliding grooves, and therefore the container above the supporting plate is centered and fixed through the sliding blocks.

Further, drive mechanism includes the transmission case, transmission case and mount pad fixed connection, and the below of transmission case is equipped with the motor, motor and mount pad fixed connection, and motor electric connection has the electromagnetic governor, and the output of motor runs through the bottom of transmission case and installs driving pulley, and driving pulley passes through the belt and is connected with the driven pulley transmission.

Furthermore, the transmission case is provided with an inwards concave cavity, the gear ring is positioned in the cavity and fixedly connected with the transmission case, and the main shaft penetrates through the cavity of the transmission case and is rotatably connected with the transmission case.

Further, the bottom end of the auxiliary shaft penetrates through the sealing bowl and is in rotary connection with the sealing bowl.

Further, homogenate subassembly is equipped with the homogenate axle including fixed pipe, the inside of fixed pipe, is equipped with the bearing between homogenate axle and the fixed pipe, and the top of homogenate axle is equipped with the lug, and the screw is installed to the bottom of homogenate axle, and open the below of fixed pipe has a plurality of evenly distributed's feed inlet, and the bottom fixedly connected with homogenate head of fixed pipe, the screw is located inside the homogenate head, and the diameter that the screw arrives is less than the internal diameter of homogenate head, makes the screw can demolish washing and disinfection after using, and the week side of homogenate head is opened the discharge gate that has a plurality of evenly distributed, and the screw can inhale the homogenate head with the material from the feed inlet when rotatory, through the screw high-speed shearing, polish and decompose after, the material disperses away from the discharge gate of homogenate head.

Furthermore, the top of fixed pipe and the bottom fixed connection of sealing bowl, the lug cooperatees with the recess for fixed pipe is along with sealing bowl pivoted while, and its inside homogenate axle also can rotate.

Has the advantages that:

the sliding blocks which can be connected in a sliding mode are arranged in the sliding grooves of the tray, the connecting disc is driven to move up and down through the expansion and contraction of the air cylinder, and the sliding blocks are driven to slide close to or away from each other in the sliding grooves through the transmission of the connecting rods, so that the sliding blocks can stably center and fix the containers on the supporting plate; the adjusting gear is driven to rotate by shaking the handle, and the adjusting gear is meshed with the rack, so that the sliding sleeve can move up and down along the sliding column, and the height position of the whole clamping mechanism can be easily adjusted to meet the use requirements of different containers;

according to the invention, the stirring mechanism is arranged, the main shaft drives the connecting frame to rotate, the auxiliary shaft on the connecting frame can rotate at a high speed on the connecting frame due to the meshing connection of the first gear and the gear ring while rotating along with the connecting frame, and the arrangement of the two second gears can enable the whole stirring mechanism to be more stable in the process of high-speed rotation, so that the problem of overlarge vibration of the stirring mechanism is avoided; after the homogenizing assembly is installed, the fixing pipe is fixedly connected with the bottom end of the sealing bowl, the homogenizing shaft is driven by the matching of the convex block and the groove, the propeller is driven by the homogenizing shaft to rotate at a high speed, materials to be processed are sheared, polished and decomposed in the homogenizing head, and are stirred by the rotating homogenizing assembly after being dispersed out of a discharge port of the homogenizing head, so that a better homogenizing effect can be achieved in a short time, the homogenizing efficiency is improved, and the nucleic acid extraction time is shortened;

after the container is clamped and positioned, the nucleic acid homogenizing and extracting operation can be very simply realized by setting the numerical value of the electromagnetic speed regulator connected with the motor, and the problem that the traditional homogenizer is complex to operate is solved.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.

FIG. 1 is a schematic diagram showing the structure of a controllable electromagnetic homogenizer for nucleic acid production according to the present invention;

FIG. 2 is a schematic view of the frame assembly and adjustment mechanism;

FIG. 3 is a schematic structural view of the clamping mechanism;

FIG. 4 is a cross-sectional view of the drive mechanism and the agitation mechanism;

FIG. 5 is a schematic structural view of a stirring mechanism;

FIG. 6 is a schematic diagram of the construction of the homogenizing block.

In the drawings, the components represented by the respective reference numerals are listed below:

1. a rack assembly; 101. a base; 102. a traveler; 103. a rack; 104. a limiting ring; 2. an adjustment mechanism; 201. a sliding sleeve; 202. an adjusting gear; 203. a handle; 204. a bolt; 205. a support; 3. a clamping mechanism; 301. a tray; 302. a chute; 303. a slider; 304. a connecting rod; 305. a connecting disc; 306. a cylinder; 4. a transmission mechanism; 401. a transmission case; 402. a motor; 403. a driving pulley; 5. a stirring mechanism; 501. a toothed ring; 502. a main shaft; 503. a driven pulley; 504. a connecting frame; 505. sealing the bowl; 506. a counter shaft; 5061. a groove; 507. a first gear; 508. a second gear; 6. a homogenizing assembly; 601. a fixed tube; 6011. a feed inlet; 602. a homogenizing shaft; 6021. a bump; 603. a bearing; 604. homogenizing a head; 6041. a discharge port; 605. a propeller; 7. and (7) mounting a seat.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1 and 2, the invention is a controllable electromagnetic homogenizer for nucleic acid preparation, which comprises a rack assembly 1, wherein a sliding column 102 is fixed on the upper surface of a base 101 of the rack assembly 1, two limiting rings 104 are sleeved on the sliding column 102, the limiting rings 104 are fixedly connected with the sliding column 102, a rack 103 is arranged between the two limiting rings 104, the rack 103 is parallel to the sliding column 102 and is fixedly connected with the sliding column 102, an adjusting mechanism 2 is arranged on the sliding column 102, a clamping mechanism 3 is arranged on one side of the adjusting mechanism 2, a mounting seat 7 is fixed at the top end of the sliding column 102, a transmission mechanism 4 is arranged at the top end of the mounting seat 7, a stirring mechanism 5 is arranged on the transmission mechanism 4, the stirring mechanism 5 is positioned right above the clamping mechanism 3, and a homogenizing assembly 6 is arranged below the stirring mechanism 5;

adjustment mechanism 2 includes sliding sleeve 201, sliding sleeve 201 cup joints on traveller 102 and rack 103 and with traveller 102 and rack 103 sliding connection, be equipped with adjusting gear 202 between sliding sleeve 201 and the rack 103, adjusting gear 202 meshes with rack 103 mutually, the fixed pivot in adjusting gear 202 center rotates with sliding sleeve 201 and is connected, and the one end of pivot runs through sliding sleeve 201 and installs handle 203, the one end that adjusting gear 202 was kept away from to sliding sleeve 201 is equipped with support 205, the one end and the sliding sleeve 201 fixed connection of support 205, there is bolt 204 in one side of sliding sleeve 201 still threaded connection, the threaded end of bolt 204 runs through sliding sleeve 201 and traveller 102 looks butt.

Referring to fig. 3, the clamping mechanism 3 includes a tray 301, a plurality of sliding slots 302 are formed in the tray 301, the sliding slots 302 are distributed in a circular array with the center of the tray 301 as a center of circle, a sliding block 303 is slidably connected in the sliding slots 302, a connecting disc 305 is arranged below the tray 301, a connecting rod 304 is arranged between the connecting disc 305 and the tray 301, two ends of the connecting rod 304 are respectively hinged to the bottom end of the sliding block 303 and the peripheral side of the connecting disc 305, an air cylinder 306 is fixed at the bottom end of the connecting disc 305, an output end of the air cylinder 306 penetrates through the connecting disc 305 and is fixedly connected with the center of the bottom end of the tray 301, and the bottom end of the tray 301 is fixedly connected with the other end of the bracket 205.

Referring to fig. 4, the transmission mechanism 4 includes a transmission case 401, the transmission case 401 is fixedly connected to the mounting base 7, the transmission case 401 has a concave cavity, a motor 402 is disposed below the transmission case 401, the motor 402 is fixedly connected to the mounting base 7, and an output end of the motor 402 penetrates through a bottom end of the transmission case 401 and is provided with a driving pulley 403;

referring to fig. 4 and 5, the stirring mechanism 5 includes a gear ring 501, the gear ring 501 is located in the cavity and fixedly connected to the transmission case 401, a first gear 507 and two second gears 508 are respectively engaged in the gear ring 501, the first gear 507 and the two second gears 508 are oppositely arranged in an equilateral triangle, a connecting frame 504 is provided below the gear ring 501, top portions of two ends of the connecting frame 504 are respectively rotatably connected to the two second gears 508, the other end of the connecting frame 504 is rotatably connected to a secondary shaft 506, a top end of the secondary shaft 506 is fixedly connected to the first gear 507, a groove 5061 is provided at a bottom end of the secondary shaft 506, a sealing bowl 505 is fixedly connected to a bottom end of the connecting frame 504, a main shaft 502 is fixed at a center of the connecting frame 504, the main shaft 502 penetrates through the cavity of the transmission case 401 and is rotatably connected to the transmission case 401, a driven pulley 503 is installed at a top end of the main shaft 502, the driving pulley 403 is in transmission connection with a driven pulley 503 through a belt.

Referring to fig. 6, the homogenizing assembly 6 includes a fixed tube 601, a top end of the fixed tube 601 is fixedly connected to a bottom end of a sealing bowl 505, a homogenizing shaft 602 is disposed inside the fixed tube 601, a bearing 603 is disposed between the homogenizing shaft 602 and the fixed tube 601, a protrusion 6021 is disposed on a top end of the homogenizing shaft 602, the protrusion 6021 is engaged with a groove 5061, a propeller 605 is mounted on a bottom end of the homogenizing shaft 602, a plurality of uniformly distributed feed ports 6011 are disposed below the fixed tube 601, a homogenizing head 604 is fixedly connected to a bottom end of the fixed tube 601, the propeller 605 is disposed inside the homogenizing head 604, a diameter of the propeller 605 is smaller than an inner diameter of the homogenizing head 604, and a plurality of uniformly distributed discharge ports 6041 are disposed on a peripheral side of the homogenizing head 604.

The working principle of the invention is as follows:

when the device is used, a container containing tissue cells to be treated is flatly placed on the tray 301, the air cylinder 306 is started, the output end of the air cylinder 306 is extended, the air cylinder 306 drives the connecting disc 305 to move in the direction away from the tray 301, the connecting disc 305 drives the sliding blocks 303 to slide in the sliding grooves 302 in a mutually approaching mode through the connecting rods 304, so that the container on the tray 301 is positioned and clamped, the circle center of the container can be positioned and overlapped with the circle center of the tray 301, the container is prevented from deviating, and the homogenizing assembly 6 is prevented from hitting the container;

after the container is clamped, firstly, the bolt 204 is rotated and loosened, the handle 203 is shaken, the handle 203 drives the adjusting gear 202 to rotate through the rotating shaft, the adjusting gear 202 is meshed with the rack 103 to drive the sliding sleeve 201 to move up and down along the sliding column 102, the sliding sleeve 201 drives the tray 301 to move up and down through the support 205, so that the homogenizing head 604 is positioned in the container, a sufficient distance is ensured between the homogenizing head 604 and the bottom end of the inner wall of the container, and then the bolt 204 is rotated and screwed down to fix the height position of the tray 301;

after the tray 301 is positioned, the rotation speed of the motor 402 is set through an electromagnetic speed regulator, the output end of the motor 402 drives the driving pulley 403 to rotate, the driving pulley 403 drives the driven pulley 503 to rotate through a belt, the driven pulley 503 drives the connecting frame 504 to rotate through the main shaft 502, the connecting frame 504 drives the auxiliary shaft 506 and the sealing bowl 505 to rotate at a low speed by centering on the main shaft 502, the sealing bowl 505 drives the whole homogenizing assembly 6 to stir at a low speed in the container, the first gear 507 on the auxiliary shaft 506 and the two second gears 508 on the connecting frame 504 are respectively meshed with gear teeth in the gear ring 501, the first gear 507 drives the auxiliary shaft 506 to rotate at a high speed by centering on the auxiliary shaft 506, the auxiliary shaft 506 drives the homogenizing shaft 602 to rotate at a high speed through the groove 5061 and the bump 6021, the homogenizing shaft 602 drives the propeller 605 at the bottom end to rotate at a high speed, so that the material in the container is sucked into the homogenizing head 604 from the feeding port 6011, and is sheared in the homogenizing head 604, Grinding and decomposing, breaking cells, decomposing macromolecular proteins, dispersing from a discharge port 6041 of a homogenizing head 604, and extracting the required nucleic acid;

after homogenate is extracted, the motor 402 is closed, the sliding adjusting mechanism 2 slides down to enable the output end of the air cylinder 306 to contract, the sliding block 303 is away from each other, the container is automatically fixed, and the container can be conveniently taken down from the tray 301 to complete the whole homogenate process.

In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The foregoing is merely exemplary and illustrative of the principles of the present invention and various modifications, additions and substitutions of the specific embodiments described herein may be made by those skilled in the art without departing from the principles of the present invention or exceeding the scope of the claims set forth herein.

Claims

1. A controllable electromagnetism refiner is used in nucleic acid preparation which characterized in that: the device comprises a rack assembly (1), wherein an adjusting mechanism (2) is installed on the rack assembly (1), a clamping mechanism (3) is installed on one side of the adjusting mechanism (2), an installation seat (7) is installed above the rack assembly (1), a transmission mechanism (4) is installed at the top end of the installation seat (7), a stirring mechanism (5) is installed on the transmission mechanism (4), and a homogenizing assembly (6) is installed below the stirring mechanism (5);

stirring mechanism (5) are including ring gear (501), ring gear (501) inner gearing has first gear (507) and two second gear (508), the below of ring gear (501) is equipped with link (504), the center of link (504) is fixed with main shaft (502), link (504) rotate with second gear (508) and are connected, still rotate on link (504) and be connected with countershaft (506), the top and first gear (507) fixed connection of countershaft (506), the bottom fixedly connected with sealing bowl (505) of link (504).

2. The controllable electromagnetic homogenizer for nucleic acid production according to claim 1, wherein: the rack assembly (1) comprises a base (101), a sliding column (102) is fixed on the base (101), a rack (103) is fixed on the sliding column (102), and the top end of the sliding column (102) is fixedly connected with a mounting seat (7).

3. The controllable electromagnetic homogenizer for nucleic acid production according to claim 2, wherein: a sliding sleeve (201) of the adjusting mechanism (2) is sleeved on a sliding column (102) and a rack (103) and is in sliding connection with the sliding column (102) and the rack (103), an adjusting gear (202) is meshed on the rack (103), the adjusting gear (202) is rotatably connected with the sliding sleeve (201) through a rotating shaft, a handle (203) is installed at one end of the rotating shaft, and a support (205) is fixed at one end of the sliding sleeve (201).

4. A controlled electromagnetic homogenizer for nucleic acid production according to claim 3, wherein: one side of the sliding sleeve (201) is in threaded connection with a bolt (204), and the threaded end of the bolt (204) penetrates through the sliding sleeve (201) and is abutted to the sliding column (102).

5. A controlled electromagnetic homogenizer for nucleic acid production according to claim 3, wherein: support (205) and tray (301) fixed connection of clamping mechanism (3), it has spout (302) to open on tray (301), sliding connection has slider (303) in spout (302), the below of tray (301) is equipped with connection pad (305), be equipped with connecting rod (304) between connection pad (305) and tray (301), the both ends of connecting rod (304) are articulated with the bottom of slider (303) and the week side of connection pad (305) respectively, the bottom mounting of connection pad (305) has cylinder (306), the output of cylinder (306) runs through connection pad (305) and with the bottom center fixed connection of tray (301).

6. The controllable electromagnetic homogenizer for nucleic acid production according to claim 1, wherein: the mounting seat (7) is fixedly connected with a transmission box (401) of the transmission mechanism (4), a motor (402) is fixed on the mounting seat (7), and an output shaft of the motor (402) is in belt transmission with the main shaft (502).

7. A controlled electromagnetic homogenizer for nucleic acid production according to claim 6, wherein: the transmission case (401) is fixedly connected with the gear ring (501), and the main shaft (502) penetrates through the transmission case (401) and is rotatably connected with the transmission case (401).

8. The controllable electromagnetic homogenizer for nucleic acid production according to claim 1, wherein: the bottom end of the auxiliary shaft (506) penetrates through the sealing bowl (505) and is rotatably connected with the sealing bowl (505).

9. The controllable electromagnetic homogenizer for nucleic acid production according to claim 1, wherein: homogenate subassembly (6) are including fixed pipe (601), rotate through bearing (603) in fixed pipe (601) and are connected with homogenate axle (602), and screw (605) are installed to the bottom of homogenate axle (602), and open the below of fixed pipe (601) has feed inlet (6011), and the bottom mounting of fixed pipe (601) has homogenate head (604), and the week side of homogenate head (604) is opened there is discharge gate (6041).

10. A controlled electromagnetic homogenizer for nucleic acid production according to claim 9, wherein: the top end of the fixed pipe (601) is fixedly connected with the bottom end of the sealing bowl (505), the bottom end of the auxiliary shaft (506) is provided with a groove (5061), the top end of the homogenizing shaft (602) is provided with a lug (6021), and the lug (6021) is matched with the groove (5061).