CN113577001B - 一种鲜石斛抗氧化组合物、抗氧化物制备方法及应用 - Google Patents
一种鲜石斛抗氧化组合物、抗氧化物制备方法及应用 Download PDFInfo
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Abstract
本发明涉及一种鲜石斛抗氧化组合物、抗氧化物制备方法及应用,鲜石斛抗氧化组合物包括鲜石斛20‑40份,葛根10‑20份、薏仁10‑20份,藏红花2‑5份,人参5‑10份、甘草5‑10份;取新鲜铁皮石斛,打碎后与鲜石斛抗氧化组合物的其他成分混合,水煎提取后取滤液,浓缩后得到抗氧化物,该抗氧化物能够用于制作化妆品。本发明的有益效果是:鲜石斛组合物提取得到的抗氧化物对.OH自由基的清除能力较高。
Description
技术领域
本发明属于生物制剂领域,尤其是涉及一种鲜石斛抗氧化组合物、抗氧化物制备方法及应用。
背景技术
随着人们年龄的增长,身体各个器官和细胞都会逐渐衰老,作为人体第一屏障的皮肤首当其冲,尤其是爱美女士对皮肤的保养尤为重要。其实衰老就是一种细胞氧化过程,因此需要对抗整个过程,抗氧化就是任何以低浓度存在就能有效抑制自由基的氧化反应的物质,其作用机理可以是直接作用在自由基,或是间接消耗掉容易生成自由基的物质,防止发生进一步反应。人体在不可避免地产生自由基的同时,也在自然产生着抵抗自由基的抗氧化物质,以抵消自由基对人体细胞的氧化攻击。研究证明,人体的抗氧化***是一个可与免疫***相比拟的、具有完善和复杂功能的***,机体抗氧化的能力越强,就越健康,当机体抗氧化***出现问题时,必须有一种能抗氧化的配方针对此种问题。
做为中华瑰宝的中草药源于自然,以中医理论为指导,可以解决以上问题。铁皮石斛(Dendrobium huoshanense)是我国名贵中药材,《本草纲目》记载其具有滋阴清热、益胃生津、润肺止咳等功效。能大幅度提高人体内SOD(延缓衰老的主要物质)水平,对经常熬夜、用脑、烟酒过度,体虚乏力的人群,经常饮用非常适宜。铁皮石斛有明目作用,也能调和阴阳、壮阳补肾、养颜驻容,从而达到保健益寿的功效。铁皮石斛对生长环境要求十分严格,多附生于悬崖峭壁和高大的乔木上,开花多,结果少,自然条件下种子发芽率极低,生长缓慢并且对温湿度要求较高。由于近年来以铁皮石斛为原料开发的保健品及化妆品日益走俏。
发明内容
为解决上述技术问题,本发明提供一种鲜石斛抗氧化组合物、抗氧化物制备方法及应用。
本发明采用的技术方案是:一种鲜石斛抗氧化组合物,包括鲜石斛20-40份,葛根10-20份、薏仁10-20份,藏红花2-5份,人参5-10份、甘草5-10份。
一种抗氧化物制备方法,取新鲜铁皮石斛,打碎后与鲜石斛抗氧化组合物的其他成分混合,水煎提取后取滤液,浓缩后得到抗氧化物。
优选地,水提两次,第一次水提料液比为1:20-25,提取时间为2-3h,第二次料液比为1:10-15,提取时间0.5-1h。
优选地,滤液浓缩至含1kg生药/L浓缩液,冷冻干燥后以冻干粉形式保存。
抗氧化物制备方法制备得到的抗氧化物在化妆品中的应用。
本发明具有的优点和积极效果是:鲜石斛组合物提取得到的抗氧化物对.OH自由基的清除能力较高,用于化妆品时具有较佳的抗氧化作用,不会对皮肤产生刺激,同时具有温养功能。
附图说明
图1各组分对DPPH·自由基清除率;
图2各组分对.OH自由基清除率。
具体实施方式
本发明涉及一种鲜石斛抗氧化组合物、抗氧化物制备方法及应用。鲜石斛抗氧化组合物,包括鲜石斛20-40份,葛根10-20份、薏仁10-20份,藏红花2-5份,人参5-10份、甘草5-10份。
石斛:是我国名贵中药材,《本草纲目》记载其具有滋阴清热、益胃生津、润肺止咳等功效。能大幅度提高人体内SOD(延缓衰老的主要物质)水平,对经常熬夜、用脑、烟酒过度,体虚乏力的人群,经常饮用非常适宜。铁皮石斛有明目作用,也能调和阴阳、壮阳补肾、养颜驻容,从而达到保健益寿的功效。铁皮石斛的主要有效成分及保健作用近年来,众多学者对铁皮石斛的有效成分及作用进行了研究,主要含有多糖、氨基酸、生物碱及多种无机元素,具有抗衰老、抗肿瘤、抗氧化、降血糖等作用。其中多糖是铁皮石斛的主要有效成分,多糖成分能够清除自由基,具有抗氧化活性,可以调节机体免疫力,防治亚健康,减少疾病的产生,抑制肿瘤,延缓衰老。
鲜品石斛:石斛是传统鲜药,中医认为石斛具有“生者优良”的特点,讲究“鲜品为上”。现代研究也表明鲜石斛比干品具有更显著的药理活性,因此,2020版《中国药典》将石斛饮片的干品和鲜品分开介绍。目前关于石斛的化学成分及药理活性的研究多集中于石斛干品,缺乏鲜、干石斛在化学成分和功效活性上差异的***深入研究,限制了鲜石斛的市场应用和产品开发。铁皮石斛鲜条直接使用,可最大限度地保持原本的有效成分;而干品则需要进行一定的加工处理,或多或少会影响铁皮石斛原有的有效成分的完整度。
葛根:葛根的主要成分是淀粉,此外含有约12%的黄酮类化合物,并且含有人体需要的十多种氨基酸和十多种微量元素。至于丰胸美颜的保健价值,是因为葛根中的异黄酮是目前在分子结构上与人体自身分泌的***最为相似的一种植物***,因此,它能与身体各***、器官组织细胞表面的***受体结合,发挥效应。其它调节女性内分泌的药物相比,葛根异黄酮的独特之处在于具有与体内***受体抢先结合和作用温和两个特点。市面上常见的葛根粉等保健美容品,多是经过加工,已经和其他成分调和在一起,在质量保证的前提下可以适当使用。
藏红花:据《本草纲目》记载,藏红花能“活血,主心气忧郁,又治惊悸”。藏红花具有疏经活络、通经化淤、散淤开结、消肿止痛、凉血解毒等诸多功效。特别是对那些经常“郁闷”的人,长期坚持服用可让人情绪平缓,并全面提高人体的免疫力。由于藏红花可以治疗血亏体虚、***等病症,所以对女性体内调理大有裨益,内里健康了,自然满面红光,所以,藏红花一直是女性保健护肤的热门。
人参:含有100多种有效成分,主要为人参皂甙、少量挥发油、各种氨基酸和人参多肽、葡萄糖、果糖、果胶以及维生素B1、B2、烟酸、泛酸等等。其中人参皂甙是最主要的活性物质,具有抗老化、抗感染的作用。人参的渗出液对皮肤没有任何不良刺激,还能有效抵抗胆固醇,帮助新陈代谢,促进皮肤血液循环,增强皮肤营养,防止皮肤脱水、硬化、起皱,从而保持皮肤角质层中的水分,令肌肤嫩滑富有弹性。人参中还含有能较强的抑制黑色素沉着的酶(酪氨酸酶),可以增白祛斑。
薏仁:是五谷类中纤维质最高的,低脂、低热量,对下半身水肿的人有奇效,是减肥的最佳主食。更重要的是其富含蛋白质,可以协助消除班点,对面部粉刺及皮肤粗糙的有明显的疗效,同时还可以防止脱发,甚至有瘦脸效果,薏仁的提炼物加入化妆品中还可达到防晒和防紫外线的效果,具有自然美白效果能提高肌肤新陈代谢与保湿的功能。用于加强肌肤的保湿屏障,预防未来色素沉淀的发生。
甘草:可补脾益气,清热解毒,祛痰止咳,缓急止痛。甘草是中最有合作精神,是中药中应用最广泛的药物之一,可以调和诸药,且药性和缓,默默地发挥自己的功效。现在许多洁面、护肤产品中含有甘草成分,我们常见的“草本配方”便是采用了甘草作为其灵魂成分,美白功效显著,且可以用来安抚受损、敏感的肌肤,同时还能提升肌肤的自我修护能力,让肌肤由内而外呈现健康肌肤的纹理与肤触。
本方案采用鲜石斛、葛根、薏仁、藏红花、人参和甘草组合成方。石斛甘,微寒,益胃生津,滋阴清热,为方中君药,起滋阴退热调和阴阳之功。葛根甘、辛,凉,解肌退热,生津止渴,透疹,升阳止泻,通经活络,为方中臣药,行气升阳协助石斛滋阴而不滞。人参甘、微苦,微温。大补元气,复脉固脱,补脾益肺,生津养血,安神益智,为方中佐药,重在补气补阳,气行而不散。薏苡仁甘、淡,凉,利水渗湿,健脾止泻,除痹,排脓,解毒散结,为方中佐药有祛湿排毒之功。藏红花甘,平,活血化瘀,凉血解毒,解郁安神。为方中佐药,调理机体气血。甘草甘,平,补脾益气,清热解毒,调和诸药。以上诸药共奏料理气血阴阳之功,改善人体机能;各组分相互协作,用于化妆品时,除了具有更好的抗氧化作用,还具有突出的滋养功能。
对上述组合物的有效成分进行提取得到抗氧化物,为了保证抗氧化物的抗氧化能力,本方案中选用了新鲜铁皮石斛。抗氧化物制备方法,取新鲜铁皮石斛,打碎取出待用;将处理好的石斛放入提取装置中,同时将其他中药饮片按比例同时加入,并加蒸馏水水煎提取,第1次料液比1:20-25,提取时间2-3h,第2次料液比1:10-15,提取时间0.5-1h,共提取2次;提取液过滤(100目),合并滤液;将滤液温度60℃以下真空减压浓缩,浓缩至含1kg生药/L浓缩液,得到抗氧化物;可冷冻干燥后以冻干粉形式存储。制备得到的抗氧化物具有较佳的自由基清除能力,可用于制备化妆品。
下面结合具体实施例对本发明方案做出说明,其中,未具体说明操作步骤的实验方法,均按照相应商品说明书进行,实施例中所用到的仪器、试剂、耗材如无特殊说明,均可从商业公司购买得到。
实施例1:抗氧化物的制备
配方:鲜石斛(20-40份),葛根(10-20份)、薏仁(10-20份),藏红花(2-5份),人参(5-10份)、甘草(5-10份)。
选取新鲜铁皮石斛,打碎取出待用。将处理好的石斛放入提取装置中,同时将其他中药饮片同时加入,并加蒸馏水水煎提取,第1次料液比1:20,提取时间2h,第2次料液比1:15,提取时间1h,共提取2次。提取液过滤(100目),合并滤液。将滤液真空减压浓缩,温度60℃以下。浓缩至含1kg生药/L浓缩液,冷冻干燥得冻干粉。
对比例1:
选取新鲜铁皮石斛,打碎取出待用。将处理好的石斛放入提取装置中,加蒸馏水水煎提取,第1次料液比1:20,提取时间2h,第2次料液比1:15,提取时间1h,共提取2次。提取液过滤(100目),合并滤液。将滤液真空减压浓缩,温度60℃以下。浓缩至含1kg生药/L浓缩液为鲜石斛提取物,冷冻干燥得冻干粉。
对比例2:
选取同一产地干品铁皮石斛,按照对比例1中的制备方法制备得到干石斛提取物。
实施例2:DPPH·自由基清除实验
分别对实施例1、对比例1和对比例2精密称定0.1g原料冻干粉,于避光环境下,加甲醇溶解并配制成浓度为0.1mmol/L的DPPH甲醇溶液。首先精密量取1mL的甲醇和1mL的双蒸水,经充分混合均匀,混合液置于比色皿中,在紫外分光光度计上于517nm波长处,作为空白液,并进行调零;精密量取1.0mL0.1mmol/LDPPH甲醇溶液,再精密量取1.0mL不同浓度的各样品溶液加入其中,立即充分混匀,在室温的条件下,使其避光反应30min,在同等条件下,测定并记录其吸光度值,并标记为At;同时设置Ac管,精密量取1.0mL0.1mmol/LDPPH甲醇溶液和1.0mL双蒸水混合,充分混匀,同法处理阳性对照药L-抗坏血酸,测定并记录吸光度值为Ac。各样品的每个浓度溶液均做3组平行试验,以计算出的平均清除率作为最终实验结果,实验结果如图1和表1所示。
表1各组分对DPPH·自由基清除率
浓度(mg/ml) | 干石斛(%) | 鲜石斛(%) | 组合物(%) | L-抗坏血酸(%) |
0.5 | 26.36 | 37.68 | 47.76 | 77.76 |
1 | 29.45 | 40.22 | 53.54 | 80.93 |
1.5 | 35.78 | 45.16 | 61.24 | 86.35 |
2 | 38.89 | 47.85 | 64.79 | 88.57 |
2.5 | 42.37 | 52.36 | 68.89 | 91.63 |
由图1和表1数据能够看出,相对于单独的干石斛提取物和鲜石斛提取物,组合物具有更强的DPPH自由基清除能力,在一定范围内,随着样品浓度增大,清除作用也逐渐增强。组合物样品清除率优于单用石斛的样品,清除率范围47.76%-68.89%;通过本实验结果表明,组合物抗氧化提取物在清除DPPH自由基能力方面,具有一定优势。
实施例3:.OH自由基清除实验
OH自由基是由生物机体产生的高活性、毒性强的一种自由基,对生物大分子、细胞等具有极大的损伤作用,.OH自由基的清除能力是常见的评价和衡量抗氧化物质的抗氧化能力强弱的重要指标之一。
分别对实施例1、对比例1和对比例2精密量取1.0mL7.5mmol/L FeSO4、1.0mL7.5mmol/L邻二氮菲、2.0mLPH7.4磷酸盐缓冲溶液、1.0mL8.8mmol/L H2O2,立即混合,并充分混匀,再加1.0mL不同浓度各样品溶液和4.0mL双蒸水,混匀,在37℃反应30min;同法制备对照样品L-抗坏血酸,用紫外分光光度计于536nm波长下测定并记录吸光度值。
表2各组分对.OH自由基清除率
浓度(mg/ml) | 干石斛(%) | 鲜石斛(%) | 组合物(%) | L-抗坏血酸(%) |
1 | 5.65 | 8.23 | 16.45 | 21.36 |
2 | 6.93 | 11.21 | 21.93 | 28.88 |
3 | 7.54 | 14.73 | 27.55 | 36.53 |
4 | 8.55 | 17.47 | 32.87 | 42.95 |
5 | 9.76 | 21.34 | 38.65 | 48.64 |
如表2和图2所示,各样品的清除能力,均随着浓度的增大而逐渐增强,组合物的清除能力优于干石斛和鲜石斛样品提取物。在相同的浓度范围内,组合物样品对·OH自由基的清除率范围在16.45-38.56%。因此,本试验结果表明,组合物对.OH自由基清除的能力有一定优势。
如实施例2和实施例3数据显示,组合物的抗氧化能力虽然在效果上还是低于具有抗氧化功能的L-抗坏血酸,但是本组合物为中药提取物,其制备过程更为简单,并非化学合成;用于化妆品时,不易产生过敏现象,尤其针对皮肤敏感的使用者,中药提取物会更为亲和。
以上对本发明的实施例进行了详细说明,但所述内容仅为本发明的较佳实施例,不能被认为用于限定本发明的实施范围。凡依本发明申请范围所作的均等变化与改进等,均应仍归属于本发明的专利涵盖范围之内。
Claims (4)
1.一种鲜石斛抗氧化组合物,其特征在于:能够用于清除.OH自由基和DPPH·自由基,由鲜铁皮石斛20-40份,葛根10-20份、薏仁10-20份,藏红花2-5份,人参5-10份、甘草5-10份组成;其中,鲜石斛为方中君药,葛根为方中臣药,人参、薏仁和藏红花为方中佐药,甘草调和诸药;
取新鲜铁皮石斛,打碎后与其他成分混合,水提两次,第一次水提料液比为1:20-25,提取时间为2-3h,第二次料液比为1:10-15,提取时间0.5-1h,水煎提取后取滤液,浓缩后得到抗氧化物。
2.一种抗氧化物制备方法,其特征在于:取新鲜铁皮石斛,打碎后与鲜石斛抗氧化组合物的其他成分混合,鲜石斛抗氧化组合物由鲜铁皮石斛20-40份,葛根10-20份、薏仁10-20份,藏红花2-5份,人参5-10份、甘草5-10份组成;
水提两次,第一次水提料液比为1:20-25,提取时间为2-3h,第二次料液比为1:10-15,提取时间0.5-1h;
水煎提取后取滤液,浓缩后得到抗氧化物。
3.根据权利要求2所述的抗氧化物制备方法,其特征在于:滤液浓缩至含1kg生药/L浓缩液,冷冻干燥后以冻干粉形式保存。
4.权利要求2或3所述的抗氧化物制备方法制备得到的抗氧化物在化妆品中的应用。
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