CN113576999A - Nourishing and repairing freeze-dried powder and preparation method thereof - Google Patents
Nourishing and repairing freeze-dried powder and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a nourishing and repairing freeze-dried powder and a preparation method thereof, and relates to the technical field of freeze-dried powder preparation. The composite material comprises the following raw materials in parts by weight: deionized water, trehalose, sodium chloride, sodium hyaluronate, glutathione, 1, 2-propylene glycol, vitamins, aloe extract, a freeze-drying protective agent, glycine, dipeptide-4 and nonapeptide-1. The addition of the trehalose, the dipeptide-4 and the nonapeptide-1 further improves the skin repair capacity, increases the generation of intercellular bonds of skin, reduces the damage of the skin, compacts the skin, stimulates the formation of collagen and elastin, compacts the skin and reduces fine lines.
Description
Technical Field
The invention relates to the technical field of freeze-dried powder, and particularly relates to nourishing and repairing freeze-dried powder and a preparation method thereof.
Background
The freeze-dried powder is an aseptic powder injection prepared by freezing a liquid medicine into a solid state in an aseptic environment, vacuumizing to sublimate and dry water, wherein the freeze-dried powder is prepared by freezing the water in the liquid medicine in advance by adopting a vacuum freeze-drying method of a freeze dryer, and then sublimating the frozen water in the liquid medicine in a vacuum aseptic environment to obtain freeze drying.
In the prior art, the repair effect of the freeze-dried powder is general in the using process, so that the using effect of the freeze-dried powder is reduced, and meanwhile, the nourishing effect of the existing freeze-dried powder is general in the preparation process.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a nourishing and repairing freeze-dried powder and a preparation method thereof, and aims to solve the problems in the background art.
In order to achieve the purpose, the invention provides the following technical scheme: the nourishing and repairing freeze-dried powder is characterized by comprising the following raw materials in parts by weight: 60-70 parts of deionized water, 1-3 parts of trehalose, 2-4 parts of sodium chloride, 5-8 parts of sodium hyaluronate, 2-3 parts of glutathione, 3-6 parts of 1, 2-propylene glycol, 1-3 parts of vitamin, 3-8 parts of aloe extract, 1-5 parts of freeze-drying protective agent, 3-4 parts of glycine, 41-3 parts of dipeptide-41 and 11-2 parts of nonapeptide.
A preparation method of a nourishing and repairing freeze-dried powder comprises the following steps:
s1, preparation of stock solution: weighing raw materials according to a ratio, placing trehalose, sodium chloride, sodium hyaluronate and deionized water in a reaction kettle, and uniformly stirring to obtain a required stock solution for later use;
s2, mixing and stirring treatment: sterilizing the stock solution prepared in the step S1 under high pressure at the temperature of 115-125 ℃ for 25-35min, cooling to 35-45 ℃, sequentially adding glutathione, 1, 2-propylene glycol, vitamins, an aloe extracting solution and glycine, placing the mixture into a reaction kettle, stirring for 6-14min at the rotation speed of 450-550rpm, and uniformly mixing to obtain a required mixed solution for later use;
s3, filtering: filtering the mixed solution prepared in the step S2 by using a filtering membrane to remove insoluble impurities;
s4, filling treatment: filling the mixed solution filtered in the step S3 into an inner cavity of a penicillin bottle, adding a freeze-drying protective agent into the penicillin bottle, and performing half-stoppering treatment on the penicillin bottle;
s5, freeze drying treatment: putting the penicillin bottle filled in the step S4 into a vacuum freeze dryer, keeping a vacuum environment, filling a proper amount of carbon dioxide protective gas, setting parameters of the freeze dryer, starting the freeze dryer, and performing pre-freezing treatment;
s6, preparation of a freeze-dried powder finished product: under the high vacuum condition, carrying out the pre-freezing treatment in the step S5, carrying out sublimation drying by using microwave heating to obtain a semi-finished product of freeze-dried powder, carrying out analysis drying by using microwave heating under the vacuum condition, and removing adsorption water to obtain a finished product of freeze-dried powder;
s7, packaging and packaging: and (5) performing gland packaging and labeling treatment on the freeze-dried powder finished product prepared in the step (S6), performing freeze drying, and performing sealed packaging under vacuum.
Further optimizing the technical scheme, in the step S3, a filtering membrane with the diameter of 0.20-0.24 μm is adopted for filtering treatment.
Further optimizing the technical scheme, the filling amount of the penicillin bottle in the step S4 is 2 ml.
Further optimizing the technical scheme, the sterilization and drying treatment is carried out on the penicillin bottle in the step S4 before filling.
Further optimizing the technical scheme, the rotation speed of the stirring in the step S1 is 500-700rpm/min, and the stirring time is 8-12 min.
Further optimizing the technical scheme, the vacuum freeze dryer in the step S5 is cooled to minus 45 ℃ to minus 40 ℃, kept warm for 4 to 8 hours, pre-freezing operation is carried out, the temperature is raised to minus 6 ℃ to minus 4 ℃ after the pre-freezing operation in the step S5, kept warm for 10 to 15 hours, freezing operation is carried out, the temperature is raised to minus 2 ℃ to 2 ℃, kept warm for 4 to 8 hours, and sublimation drying operation is carried out.
Further optimizing the technical scheme, in the step S5, the vacuum freeze dryer is heated to 40-45 ℃ and is kept warm for 4-8h, desorption drying operation is carried out to complete freeze drying operation, and the vacuum freeze dryer is vacuumized to the vacuum degree of 10-14 pa.
Further optimizing the technical scheme, the freeze-dried powder after freeze drying in the step S6 is subjected to plug pressing in a vacuum state and taken out of the pot to obtain a freeze-dried powder part.
Further optimizing the technical scheme, the lyoprotectant in the step S4 is one or a mixture of more than two of mannitol, dextran and sucrose.
Compared with the prior art, the invention provides a nourishing and repairing freeze-dried powder and a preparation method thereof, and the nourishing and repairing freeze-dried powder has the following beneficial effects:
1. the addition of trehalose, dipeptide-4 and nonapeptide-1 further improves the skin repair capacity, increases the production of intercellular mucus of skin, reduces the damage and compaction of skin, stimulates the formation of collagen and elastin, compacts skin and reduces fine lines.
2. The nourishing and repairing freeze-dried powder and the preparation method thereof adopt the vacuum freeze-drying technology, not only preserve the nutrient components of the materials, but also can effectively prolong the shelf life of the product, the addition of the aloe extract solution obviously increases the regeneration process of the skin, the addition of the sodium hyaluronate has good moisturizing effect, the preparation method in the embodiment of the invention is simple and easy to popularize, and the obtained product can be used for moisturizing and maintaining the skin of the whole body including facial care, the scheme is simple, convenient and easy to implement, is suitable for large-scale production of stem cell culture supernatant freeze-dried powder from different sources, and has high practicability.
Drawings
Fig. 1 is a flow diagram of a nourishing and repairing freeze-dried powder and a preparation method thereof provided by the invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The first embodiment is as follows: referring to fig. 1, the invention discloses a nourishing and repairing freeze-dried powder, which comprises the following raw materials in parts by weight: 68 parts of deionized water, 2 parts of trehalose, 3 parts of sodium chloride, 7 parts of sodium hyaluronate, 2 parts of glutathione, 3 parts of 1, 2-propylene glycol, 2 parts of vitamin, 7 parts of aloe extract, 4 parts of freeze-drying protective agent, 3 parts of glycine, 42 parts of dipeptide-42 and 11.5 parts of nonapeptide.
A preparation method of a nourishing and repairing freeze-dried powder comprises the following steps:
s1, preparation of stock solution: weighing raw materials according to a ratio, placing trehalose, sodium chloride, sodium hyaluronate and deionized water in a reaction kettle, and uniformly stirring at a rotation speed of 700rpm/min for 9min to obtain a required stock solution for later use;
s2, mixing and stirring treatment: sterilizing the stock solution prepared in the step S1 at 125 ℃ under high pressure for 35min, cooling to 45 ℃, sequentially adding glutathione, 1, 2-propylene glycol, vitamins, aloe extract and glycine, placing into a reaction kettle, stirring at the rotation speed of 550rpm for 8min, and uniformly mixing to obtain the required mixed solution for later use;
s3, filtering: filtering the mixed solution prepared in the step S2 by using a filtering membrane to remove insoluble impurities, and filtering by using a filtering membrane of 0.21 mu m;
s4, filling treatment: filling the mixed solution filtered in the step S3 into an inner cavity of a penicillin bottle, adding a freeze-drying protective agent into the penicillin bottle, wherein the freeze-drying protective agent is dextran, performing semi-stoppering treatment on the penicillin bottle, sterilizing and drying the penicillin bottle before filling, and the filling amount of the penicillin bottle is 2 ml;
s5, freeze drying treatment: putting the penicillin bottle filled in the step S4 into a vacuum freeze dryer, keeping a vacuum environment, filling a proper amount of carbon dioxide protective gas, setting parameters of the freeze dryer, starting the freeze dryer, performing pre-freezing treatment, cooling the vacuum freeze dryer to-45 ℃, preserving heat for 6 hours, performing pre-freezing operation, heating the pre-freezing operation in the step S5 to-6 ℃ after pre-freezing operation, preserving heat for 10-15 hours, performing freezing operation, heating to-2 ℃, preserving heat for 5 hours, performing sublimation drying operation, finally heating to 40 ℃ by the vacuum freeze dryer, preserving heat for 6 hours, performing desorption drying operation, completing the freeze drying operation, and vacuumizing the vacuum freeze dryer to the vacuum degree of 11 pa;
s6, preparation of a freeze-dried powder finished product: under the high vacuum condition, carrying out sublimation drying by microwave heating after pre-freezing treatment in the step S5 to obtain a semi-finished product of freeze-dried powder, carrying out analysis drying by microwave heating under the vacuum condition, plugging the freeze-dried powder in the vacuum state, taking out of the pot to obtain a part of the freeze-dried powder, and removing adsorption water to obtain a finished product of the freeze-dried powder;
s7, packaging and packaging: and (5) performing gland packaging and labeling treatment on the freeze-dried powder finished product prepared in the step (S6), performing freeze drying, and performing sealed packaging under vacuum.
Example two: referring to fig. 1, the invention discloses a nourishing and repairing freeze-dried powder, which comprises the following raw materials in parts by weight: 70 parts of deionized water, 2 parts of trehalose, 3 parts of sodium chloride, 5 parts of sodium hyaluronate, 2 parts of glutathione, 3-6 parts of 1, 2-propylene glycol, 2 parts of vitamin, 5 parts of aloe extract, 4 parts of freeze-drying protective agent, 3 parts of glycine, 42 parts of dipeptide-42 parts and 12 parts of nonapeptide.
A preparation method of a nourishing and repairing freeze-dried powder comprises the following steps:
s1, preparation of stock solution: weighing raw materials according to a ratio, placing trehalose, sodium chloride, sodium hyaluronate and deionized water in a reaction kettle, and uniformly stirring at a rotation speed of 600rpm/min for 10min to obtain a required stock solution for later use;
s2, mixing and stirring treatment: sterilizing the stock solution prepared in the step S1 under high pressure at the temperature of 115-125 ℃ for 30min, cooling to 45 ℃, sequentially adding glutathione, 1, 2-propylene glycol, vitamins, aloe extract and glycine, placing into a reaction kettle, stirring at the rotation speed of 550rpm for 8min, and uniformly mixing to obtain the required mixed solution for later use;
s3, filtering: filtering the mixed solution prepared in the step S2 by using a filtering membrane to remove insoluble impurities, and filtering by using a filtering membrane of 0.22 mu m;
s4, filling treatment: filling the mixed solution filtered in the step S3 into an inner cavity of a penicillin bottle, adding a freeze-drying protective agent into the penicillin bottle, wherein the freeze-drying protective agent is a mixture of dextran and sucrose, performing semi-stoppering treatment on the penicillin bottle, sterilizing and drying the penicillin bottle before filling, and the filling amount of the penicillin bottle is 2 ml;
s5, freeze drying treatment: putting the penicillin bottle filled in the step S4 into a vacuum freeze dryer, filling a proper amount of carbon dioxide protective gas after keeping a vacuum environment, setting parameters of the freeze dryer, starting the freeze dryer, performing pre-freezing treatment, cooling the vacuum freeze dryer to-40 ℃, preserving heat for 7 hours, performing pre-freezing operation, heating the pre-freezing operation in the step S5 to-4 ℃ after pre-freezing operation, preserving heat for 10 hours, performing freezing operation, heating to-2 ℃, preserving heat for 6 hours, performing sublimation drying operation, finally heating the vacuum freeze dryer to 42 ℃, preserving heat for 5 hours, performing desorption drying operation, completing freeze drying operation, and vacuumizing the vacuum freeze dryer to the vacuum degree of 12 pa;
s6, preparation of a freeze-dried powder finished product: under the high vacuum condition, carrying out sublimation drying by microwave heating after pre-freezing treatment in the step S5 to obtain a semi-finished product of freeze-dried powder, carrying out analysis drying by microwave heating under the vacuum condition, plugging the freeze-dried powder in the vacuum state, taking out of the pot to obtain a part of the freeze-dried powder, and removing adsorption water to obtain a finished product of the freeze-dried powder;
s7, packaging and packaging: and (5) performing gland packaging and labeling treatment on the freeze-dried powder finished product prepared in the step (S6), performing freeze drying, and performing sealed packaging under vacuum.
Example three: referring to fig. 1, the invention discloses a nourishing and repairing freeze-dried powder, which comprises the following raw materials in parts by weight: 60 parts of deionized water, 2 parts of trehalose, 2 parts of sodium chloride, 7 parts of sodium hyaluronate, 2 parts of glutathione, 3 parts of 1, 2-propylene glycol, 3 parts of vitamin, 6 parts of aloe extract, 3 parts of freeze-drying protective agent, 3 parts of glycine, 41 parts of dipeptide-41 parts and 12 parts of nonapeptide.
A preparation method of a nourishing and repairing freeze-dried powder comprises the following steps:
s1, preparation of stock solution: weighing raw materials according to a ratio, placing trehalose, sodium chloride, sodium hyaluronate and deionized water in a reaction kettle, and uniformly stirring at a rotation speed of 700rpm/min for 11min to obtain a required stock solution for later use;
s2, mixing and stirring treatment: sterilizing the stock solution prepared in the step S1 at 125 ℃ under high pressure for 35min, cooling to 45 ℃, sequentially adding glutathione, 1, 2-propylene glycol, vitamins, aloe extract and glycine, placing into a reaction kettle, stirring at the rotation speed of 550rpm for 8min, and uniformly mixing to obtain the required mixed solution for later use;
s3, filtering: filtering the mixed solution prepared in the step S2 by using a filtering membrane to remove insoluble impurities, and filtering by using a filtering membrane of 0.24 mu m;
s4, filling treatment: filling the mixed solution filtered in the step S3 into an inner cavity of a penicillin bottle, adding a freeze-drying protective agent into the penicillin bottle, wherein the freeze-drying protective agent is mannitol, performing semi-stoppering treatment on the penicillin bottle, sterilizing and drying the penicillin bottle before filling, and the filling amount of the penicillin bottle is 2 ml;
s5, freeze drying treatment: putting the penicillin bottle filled in the step S4 into a vacuum freeze dryer, keeping a vacuum environment, filling a proper amount of carbon dioxide protective gas, setting parameters of the freeze dryer, starting the freeze dryer, performing pre-freezing treatment, cooling the vacuum freeze dryer to-45 ℃, preserving heat for 4 hours, performing pre-freezing operation, heating the pre-freezing operation in the step S5 to-4 ℃ after pre-freezing operation, preserving heat for 10 hours, performing freezing operation, heating to-2 ℃, preserving heat for 4 hours, performing sublimation drying operation, finally heating the vacuum freeze dryer to 45 ℃, preserving heat for 7 hours, performing desorption drying operation, completing freeze drying operation, and vacuumizing the vacuum freeze dryer to the vacuum degree of 14 pa;
s6, preparation of a freeze-dried powder finished product: under the high vacuum condition, carrying out sublimation drying by microwave heating after pre-freezing treatment in the step S5 to obtain a semi-finished product of freeze-dried powder, carrying out analysis drying by microwave heating under the vacuum condition, plugging the freeze-dried powder in the vacuum state, taking out of the pot to obtain a part of the freeze-dried powder, and removing adsorption water to obtain a finished product of the freeze-dried powder;
s7, packaging and packaging: and (5) performing gland packaging and labeling treatment on the freeze-dried powder finished product prepared in the step (S6), performing freeze drying, and performing sealed packaging under vacuum.
And (4) judging the standard: through comparison of the three embodiments, the best effect is the second embodiment, so that the second embodiment is selected as the best embodiment, and the specific change of the amount also belongs to the protection scope of the technical scheme.
The invention has the beneficial effects that: the addition of trehalose, dipeptide-4 and nonapeptide-1 further improves the skin repair capacity, increases the production of intercellular mucus of skin, reduces the damage of skin, compacts the skin, stimulates the formation of collagen and elastin, compacts the skin and reduces fine lines, and the freeze-dried powder prepared by the invention is suitable for deep repair after various skin damages, improves the skin quality, secretes protein, promotes the healing of deep cell tissues of the damaged skin, has good repair effect, can obviously improve facial wrinkles, and enhances the moisturizing, moisturizing and barrier functions of the face; the invention adopts the vacuum freeze-drying technology, not only preserves the nutrient components of the materials, but also can effectively prolong the shelf life of the product, the addition of the aloe extract solution can obviously increase the regeneration process of the skin, thereby being convenient for repairing the epidermal layer, promoting the synthesis of keratin and promoting the regeneration of the epidermis, the addition of the sodium hyaluronate has good moisturizing effect, the preparation method in the embodiment of the invention is simple and easy to popularize, the obtained product can be used for moisturizing and maintaining the whole body skin including facial care, can effectively inhibit skin allergy or inflammatory reaction, reduce skin sensitization, enhance the resistance of the skin to bacterial infection and promote the repair of skin barriers, and the scheme is simple, convenient and easy to implement, is suitable for the large-scale production of the dry cell culture supernatant freeze-dried powder from different sources, and has higher practicability.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. The nourishing and repairing freeze-dried powder is characterized by comprising the following raw materials in parts by weight: 60-70 parts of deionized water, 1-3 parts of trehalose, 2-4 parts of sodium chloride, 5-8 parts of sodium hyaluronate, 2-3 parts of glutathione, 3-6 parts of 1, 2-propylene glycol, 1-3 parts of vitamin, 3-8 parts of aloe extract, 1-5 parts of freeze-drying protective agent, 3-4 parts of glycine, 41-3 parts of dipeptide-41 and 11-2 parts of nonapeptide.
2. The preparation method of the nourishing and repairing freeze-dried powder as claimed in claim 1, which is characterized by comprising the following steps:
s1, preparation of stock solution: weighing raw materials according to a ratio, placing trehalose, sodium chloride, sodium hyaluronate and deionized water in a reaction kettle, and uniformly stirring to obtain a required stock solution for later use;
s2, mixing and stirring treatment: sterilizing the stock solution prepared in the step S1 under high pressure at the temperature of 115-125 ℃ for 25-35min, cooling to 35-45 ℃, sequentially adding glutathione, 1, 2-propylene glycol, vitamins, an aloe extracting solution and glycine, placing the mixture into a reaction kettle, stirring for 6-14min at the rotation speed of 450-550rpm, and uniformly mixing to obtain a required mixed solution for later use;
s3, filtering: filtering the mixed solution prepared in the step S2 by using a filtering membrane to remove insoluble impurities;
s4, filling treatment: filling the mixed solution filtered in the step S3 into an inner cavity of a penicillin bottle, adding a freeze-drying protective agent into the penicillin bottle, and performing half-stoppering treatment on the penicillin bottle;
s5, freeze drying treatment: putting the penicillin bottle filled in the step S4 into a vacuum freeze dryer, keeping a vacuum environment, filling a proper amount of carbon dioxide protective gas, setting parameters of the freeze dryer, starting the freeze dryer, and performing pre-freezing treatment;
s6, preparation of a freeze-dried powder finished product: under the high vacuum condition, carrying out the pre-freezing treatment in the step S5, carrying out sublimation drying by using microwave heating to obtain a semi-finished product of freeze-dried powder, carrying out analysis drying by using microwave heating under the vacuum condition, and removing adsorption water to obtain a finished product of freeze-dried powder;
s7, packaging and packaging: and (5) performing gland packaging and labeling treatment on the freeze-dried powder finished product prepared in the step (S6), performing freeze drying, and performing sealed packaging under vacuum.
3. The method for preparing a nourishing and repairing lyophilized powder as claimed in claim 2, wherein in step S3, a filtration membrane of 0.20-0.24 μm is used for filtration.
4. The method for preparing freeze-dried powder for nourishing and repairing according to claim 2, wherein the amount of penicillin bottles filled in step S4 is 2 ml.
5. The method for preparing freeze-dried powder for nourishing and repairing according to claim 2, wherein in the step S4, the vial is sterilized and dried before being filled.
6. The method for preparing nourishing and repairing lyophilized powder as claimed in claim 2, wherein the stirring speed in step S1 is 500-700rpm/min, and the stirring time is 8-12 min.
7. The method for preparing a nourishing and repairing freeze-dried powder according to claim 2, wherein the vacuum freeze dryer in step S5 is cooled to-45 ℃ to-40 ℃ and kept warm for 4-8h, and pre-freezing is performed, and the temperature is raised to-6 ℃ to-4 ℃ after pre-freezing in step S5, kept warm for 10-15h, and then frozen, and raised to-2 ℃ and kept warm for 4-8h, and then sublimation drying is performed.
8. The method for preparing a nourishing and repairing freeze-dried powder according to claim 2, wherein in step S5, the vacuum freeze-drying machine is heated to 40 ℃ to 45 ℃ and kept warm for 4 to 8 hours, and then the desorption drying operation is performed to complete the freeze-drying operation, and the vacuum freeze-drying machine is vacuumized to a vacuum degree of 10 to 14 pa.
9. The method for preparing a nourishing and repairing lyophilized powder of claim 2, wherein the lyophilized powder lyophilized in step S6 is subjected to vacuum state for plugging and taken out of the pan to obtain the lyophilized powder.
10. The method for preparing a nourishing and repairing lyophilized powder of claim 2, wherein the lyoprotectant in step S4 is one or a mixture of more than two of mannitol, dextran and sucrose.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111016910.5A CN113576999A (en) | 2021-08-31 | 2021-08-31 | Nourishing and repairing freeze-dried powder and preparation method thereof |
Applications Claiming Priority (1)
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114146017A (en) * | 2021-11-12 | 2022-03-08 | 广州科妍化妆品有限公司 | Freeze-dried eye patch and preparation method thereof |
| CN114948800A (en) * | 2022-06-07 | 2022-08-30 | 美尚(广州)化妆品股份有限公司 | Freeze-dried composition containing framework molecules and macromolecular carriers and application thereof |
| CN115177547A (en) * | 2022-07-20 | 2022-10-14 | 天津瑞湃尔生物科技有限公司 | MiRNA freeze-drying protective agent with repairing function and preparation method of freeze-drying balls of miRNA freeze-drying protective agent |
| CN115671058A (en) * | 2022-11-01 | 2023-02-03 | 广州博硕美医药科技有限公司 | Ring-shaped freeze-dried powder and preparation method thereof |
-
2021
- 2021-08-31 CN CN202111016910.5A patent/CN113576999A/en not_active Withdrawn
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114146017A (en) * | 2021-11-12 | 2022-03-08 | 广州科妍化妆品有限公司 | Freeze-dried eye patch and preparation method thereof |
| CN114948800A (en) * | 2022-06-07 | 2022-08-30 | 美尚(广州)化妆品股份有限公司 | Freeze-dried composition containing framework molecules and macromolecular carriers and application thereof |
| CN114948800B (en) * | 2022-06-07 | 2023-12-29 | 吴冬 | Freeze-dried composition containing skeleton molecules and macromolecular carriers and application thereof |
| CN115177547A (en) * | 2022-07-20 | 2022-10-14 | 天津瑞湃尔生物科技有限公司 | MiRNA freeze-drying protective agent with repairing function and preparation method of freeze-drying balls of miRNA freeze-drying protective agent |
| CN115671058A (en) * | 2022-11-01 | 2023-02-03 | 广州博硕美医药科技有限公司 | Ring-shaped freeze-dried powder and preparation method thereof |
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Application publication date: 20211102 |