CN113567194A - Concentrated sampling head and concentrated sample thief of microorganism aerosol are gathered to cyclone - Google Patents

Concentrated sampling head and concentrated sample thief of microorganism aerosol are gathered to cyclone Download PDF

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Publication number
CN113567194A
CN113567194A CN202110803424.1A CN202110803424A CN113567194A CN 113567194 A CN113567194 A CN 113567194A CN 202110803424 A CN202110803424 A CN 202110803424A CN 113567194 A CN113567194 A CN 113567194A
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China
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aerosol
flow
small
particle
concentrated
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CN202110803424.1A
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张健伟
张志彬
李奕新
吴清平
李文龙
段贵娇
骆延平
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Guangdong Huankai Biotechnology Co Ltd
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Guangdong Huankai Biotechnology Co Ltd
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/02Devices for withdrawing samples
    • G01N1/10Devices for withdrawing samples in the liquid or fluent state
    • G01N1/14Suction devices, e.g. pumps; Ejector devices

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  • Hydrology & Water Resources (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

The invention discloses a concentrated sampling head for collecting microbial aerosol in a cyclone manner, which comprises a shell, a virtual impact acceleration sieve pore component and a fan, wherein a small flow cavity and a main flow cavity which are mutually separated are formed in the shell; the fan is arranged in the main flow cavity and accelerates the small particle aerosol to be sucked in from the air inlet and discharged from the main flow exhaust port. Compared with the prior art, the invention has the advantages of small volume, convenient carrying, low energy consumption and large collecting suction force, and can form cyclone airflow to be input into the concentration sampling head. The invention also discloses a concentration sampler for collecting the microbial aerosol in a cyclone manner.

Description

Concentrated sampling head and concentrated sample thief of microorganism aerosol are gathered to cyclone
Technical Field
The invention relates to microorganism aerosol sampling, in particular to concentration sampling of microorganism aerosol.
Background
The microbial aerosol refers to a particle material with a particle size of 1-100 μm, wherein the particle material contains bacteria, fungi, spores, pollen, viruses, organic substances secreted by active organisms, and plant or animal debris and debris, and is a colloid system formed by microbes suspended in the air. The natural environment contains a large amount of microbial aerosol, and the microbial aerosol with the grain diameter of 0.1-20.0 μm is closely related to the health of human beings. The research and monitoring of the concentration, the type, the distribution and the change rule of the aerosol of the air microorganism have important significance.
Referring to chinese patent CN201210103840, in the conventional aerosol concentration sampler, a vacuum pump for dual-path sampling is generally adopted to respectively extract gas in a small-flow gas path and a main-flow gas path to provide air separation power. In order to ensure that the sampling flow of the main flow gas circuit and the small flow gas circuit is large enough, the vacuum sampling part above 10KG is required to be used for sampling, so that the whole sampling equipment is large in size and difficult to carry, and an external power supply is required to be connected during sampling to work by using the external power supply. On the other hand, because the suction force at the sampling head is insufficient, the sampling distance of the main flow needs to be shortened during sampling, an aluminum sampling head is required to be matched with a long sampling pipe (about 3 meters) to be connected to a vacuum pump of the main flow gas path, so that the sampling pipe with the length of about 3 meters causes great pressure drop to the main gas path, and the requirement of the large flow preset value of the sampling head part cannot be met even if the flow of the main vacuum sampling pump is sufficient; the real condition of the actual sampling air cannot be fed back really, and the long sampling tube is not beneficial to the problems of cleaning, disinfection, replacement, carrying and the like.
Therefore, a microorganism aerosol concentration and sampling device capable of solving the above problems is urgently needed.
Disclosure of Invention
The invention aims to provide a concentration sampling head and a concentration sampler for collecting microbial aerosol in a cyclone manner, which have the advantages of small volume, convenient carrying, low energy consumption and large collection suction force, and can ensure that the aerosol is input into the concentration sampling head in the cyclone manner.
In order to achieve the aim, the invention discloses a concentrated sampling head for collecting microbial aerosol in a cyclone manner, which comprises a shell, a virtual impact accelerating sieve pore component and a fan, wherein a small flow cavity and a main flow cavity which are mutually isolated, an air inlet communicated with the small flow cavity and the main flow cavity, a small flow exhaust port communicated with the small flow cavity and a main flow exhaust port communicated with the main flow cavity are formed in the shell; the virtual impact accelerating screen hole assembly is arranged at the air inlet, aerosol is sucked in a cyclone type accelerating mode and is screened to form large-particle aerosol and small-particle aerosol, the large-particle aerosol is conveyed to the small flow cavity, and the small-particle aerosol is conveyed to the main flow cavity; the fan is arranged in the main flow cavity and accelerates the small-particle aerosol to be sucked in from the air inlet and discharged from the main flow exhaust port.
Compared with the prior art, on one hand, the fan is arranged in the main flow cavity of the concentration sampling head, so that the large-flow air collection capacity can be provided for the aerosol at the air inlet, the suction speed of aerosol particles is effectively improved, the particle size separation capacity of the aerosol particles is improved, the aerosol generates air cyclone, and the aerosol at the inlet of the concentration sampling head enters the concentration sampling head in a rotating mode. On the other hand, the embedded setting of fan for fan and concentrated sampling head formula structure as an organic whole need not to set up external vacuum pump and will concentrate the major flow gas vent of sampling head and be connected to the vacuum pump through long sampling pipe, makes this concentrated sample thief small, portable, and easily washs.
Preferably, the virtual impact acceleration sieve pore assembly includes an aerosol particle acceleration plate and an aerosol particle size separation plate, the aerosol particle acceleration plate is installed at the air inlet, a plurality of first sieve pores are opened at a position on the aerosol particle acceleration plate communicated with the small flow cavity, the aerosol particle size separation plate is installed at the inner side of the aerosol particle acceleration plate at a certain interval, a flow cavity is formed between the aerosol particle acceleration plate and the aerosol particle size separation plate, a second sieve pore is opened at a position of the aerosol particle size separation plate opposite to the first sieve pore, a main vent hole is opened at a position of the aerosol particle size separation plate opposite to the main flow cavity, and a pore diameter of the second sieve pore is larger than that of the first sieve pore.
Preferably, an accelerating flow guide channel gradually downward is formed in the small flow cavity, the small flow exhaust port is located at the lowest point of the accelerating flow guide channel, and the accelerating flow guide channel accelerates the large particle aerosol to be guided and exhausted from the small flow exhaust port. On one hand, the concentration sampling head forms an acceleration flow guide channel for accelerating the concentrated and separated large particle aerosol in the small flow cavity, so that the large particle aerosol is guided and output to the small flow exhaust port more quickly, the large particle aerosol is prevented from being deposited in the small flow cavity, and a cyclone suction force is provided for the aerosol at the air inlet, so that the cyclone of the aerosol is accelerated to be increased. On the other hand, the accelerating flow guide channel is arranged in the small-flow cavity of the sampling head, so that a small-flow sampling pump can be adopted outside the small-flow exhaust port, the electric energy is less, the power consumption is low, the energy is saved, the device can be driven by a built-in lithium battery, the device can work by being separated from an external power supply, and the portability of the concentration sampler is improved.
Preferably, the small flow cavity surrounds the main flow cavity, the acceleration flow guide channel is an arc-shaped flow guide channel surrounding the main flow cavity, and an inclined arc-shaped flow guide channel is arranged outside the main flow cavity, so that aerosol can be favorably formed into cyclone input, and the cyclone area is large.
Preferably, there are two flow guide channels, and the two flow guide channels surround the main flow chamber from two sides and are communicated with each other in a cross manner at the tail ends. Cyclone formation at the air inlet is facilitated, so that aerosol is rapidly input into the air inlet of the shell in a cyclone mode and screened by the virtual impact accelerating sieve pore assembly.
Preferably, the housing is formed with a gradually inwardly shrinking bell mouth outside the virtual impact acceleration sieve pore assembly, so as to form a convergence area outside the virtual impact acceleration sieve pore assembly, thereby facilitating the generation of cyclone, accelerating the speed of aerosol entering the concentration sampling head, providing a primary acceleration area, and providing assistance for the aerosol entering the sieve pores of the virtual impact acceleration sieve pore assembly.
Preferably, the concentrated sampling head further comprises a flow sensor disposed at the main flow vent. The fan is a fan with adjustable flow. The flow of the fan can be subjected to feedback regulation by using a control unit through the flow detected by the flow sensor, so that the flow of the main flow exhaust port is stable.
The invention also discloses a concentration sampler for cyclone type collection of microbial aerosol, which comprises a concentration sampling head, a sampling pump and a sampling bottle, wherein the concentration sampling head is as above, the sampling pump is communicated with the small-flow exhaust port through a concentration gas path to extract large-particle aerosol, and the sampling bottle is arranged on the concentration gas path and collects the large-particle aerosol.
Preferably, the concentration sampler further comprises a lithium battery, and the lithium battery supplies power to the fan and the sampling pump.
Preferably, a flow sensor is arranged on the concentration gas path, and the sampling pump is a flow-adjustable pump.
Drawings
FIG. 1 is a schematic perspective view of a first angle of the concentrated sampling head of the present invention.
Fig. 2 is a schematic cross-sectional view of an inventive concentrated sampling head.
Fig. 3 is a perspective view of a second angle of the concentrated sampling head of the present invention.
Fig. 4 is a schematic front view of the housing of the concentrated sampling head of the present invention.
Fig. 5 is a schematic reverse view of the housing of the concentrated sampling head of the present invention.
FIG. 6 is a schematic diagram of the configuration of the concentrated sampler of the present invention.
Detailed Description
In order to explain technical contents, structural features, and objects and effects of the present invention in detail, the following detailed description is given with reference to the accompanying drawings in conjunction with the embodiments.
Referring to fig. 6, the invention discloses a concentration sampler 100 for collecting microbial aerosol in a cyclone manner, comprising a concentration sampling head 10, a sampling pump 20 and a sampling bottle 30, wherein the concentration sampling head 10 collects aerosol and performs concentration separation on the aerosol, the aerosol is screened into large-particle aerosol and small-particle aerosol, the large-particle aerosol is discharged from a small-flow exhaust port, and the small-particle aerosol is discharged from a main-flow exhaust port. The sampling pump 20 is communicated with the small flow exhaust port through a concentration gas path 21 to extract large particle aerosol, and the sampling bottle 30 is installed on the concentration gas path 21 and collects the large particle aerosol.
Referring to fig. 1-5, the thickening sampling head 10 includes a housing 11, a virtual impaction acceleration screen assembly 12, and a fan 13.
Referring to fig. 1 to 5, a small flow chamber 111 and a main flow chamber 112 isolated from each other, an inlet 113 communicating with the small flow chamber 111 and the main flow chamber 112, a small flow outlet 114 communicating with the small flow chamber 112, and a main flow outlet 115 communicating with the main flow chamber 112 are formed in the housing 11. The small flow vent 114 is provided with a concentrate vent port 1141, and the main flow vent 115 is provided with a main gas passage port 1151.
Referring to fig. 2, the virtual impingement acceleration screen assembly 12, installed at the air inlet 113, performs cyclonic acceleration of the aerosol into and screens out large particle aerosol and small particle aerosol, delivers the large particle aerosol to the small flow chamber 111, and delivers the small particle aerosol to the main flow chamber 112. Wherein the virtual impaction accelerating screen hole assembly 12 screens out large particle aerosol and small particle aerosol through the aerosol particle accelerating plate 121 and the aerosol particle size separating plate 122.
Referring to fig. 2, the virtual impact acceleration sieve pore assembly 12 includes an aerosol particle acceleration plate 121 and an aerosol particle size separation plate 122, the aerosol particle acceleration plate 121 is installed at the air inlet 113, a plurality of first sieve pores 1211 are opened at a position where the aerosol particle acceleration plate 121 is communicated with the small flow cavity 111, the aerosol particle size separation plate 122 is installed at an inner side of the aerosol particle acceleration plate 121 at a certain interval, and a flow cavity 123 is formed between the aerosol particle acceleration plate 121 and the aerosol particle size separation plate 122, a second sieve pore 1221 is opened at a position where the aerosol particle size separation plate 122 is opposite to the first sieve pores 1211, the aerosol particle size separation plate 122 is opened at a position where the aerosol particle size separation plate 112 is opposite to the main flow cavity 112, and a pore diameter of the second sieve pore 1221 is larger than that of the first sieve pore 1211.
The first screen holes 1211 and the second screen holes 1221 correspond to each other one by one, and the aperture of the first screen hole 1211 of the aerosol particle accelerating plate 121 is 0.71mm, thereby forming an accelerating area.
Referring to fig. 2, a blower 13 is mounted within the primary flow chamber 112 and accelerates small particle aerosols into the intake port 113 and out the primary flow exhaust port 115. The fan 13 is a fan with adjustable flow. In this embodiment, the fan 13 is a small-volume large-flow fan.
Wherein, the concentrated sampling head 10 further comprises a flow sensor 15 disposed at the main flow exhaust port. The flow sensor 15 is a digital gas flow sensor.
Referring to fig. 2 and 4, a gradually downward acceleration guide channel 14 is formed in the small flow chamber 111, the small flow exhaust port 114 is located at the lowest point of the acceleration guide channel 14, and the acceleration guide channel 14 accelerates the large particle aerosol to be guided and exhausted from the small flow exhaust port 115.
Referring to fig. 4, the small flow chamber 111 is disposed around the main flow chamber 112, and the acceleration guide 14 is an arc-shaped guide surrounding the main flow chamber 112. Two of the acceleration flow guide channels 14 are provided, and the two acceleration flow guide channels 14 surround the main flow chamber 112 from two sides and have ends in intersection communication, and the small flow exhaust port 114 is located at the intersection communication. The difference value of the acceleration guide channels 14 from top to bottom is lower, and of course, only one acceleration guide channel 14 can be arranged, and the acceleration guide channels are spirally arranged outside the arc-shaped guide channels and are in a spiral shape.
Preferably, the housing 11 is formed with a gradually inwardly shrinking bell-mouth 116 outside the aerosol particle accelerating plate 121 of the virtual impaction accelerating sieve pore assembly 12, so as to form a converging area outside the first sieve 1211 of the aerosol particle accelerating plate 121 of the virtual impaction accelerating sieve pore assembly 12, which is convenient for cooperating with the fan 13 to form a cyclone, and also makes the inlet of the sampling head 10 large and the collection area large.
In this embodiment, the shell of the bell mouth 116 and the aerosol particle accelerating plate 121 are integrally formed, and of course, the aerosol particle accelerating plate 121 may also be installed at the shell forming the bell mouth 116. The bell mouth 116 is a front end region of the intake port 113.
Preferably, the concentration sampler 100 further comprises a lithium battery 41, and the lithium battery 41 supplies power to the fan 13 and the sampling pump 20.
Wherein the sampling pump 20 is a flow-adjustable pump. The sampling pump 20 of the present embodiment employs a low-flow sampling pump. The sampling pump 20 is a diaphragm vacuum pump.
Wherein, the sampling bottle 30 comprises an air inlet nozzle, a sound velocity generation air nozzle, a collecting pipe and an air outlet nozzle. The gas to be sampled is sucked by the gas inlet nozzle; the sound velocity generating air nozzle accelerates the gas entering the collecting bottle, the gas flow is impacted in a high-speed rotating sample in the collecting liquid, and the secondary gasification and the pressure generated on the microorganism tissue are further reduced under the integration of the lateral cyclone impact and the centrifugal technology, so that the gas sample can be more effectively collected in the collecting pipe; after being collected by the collecting pipe, the gas flows to the gas outlet nozzle and is conveyed to the sampling pump 20 by a hose connected with the gas outlet nozzle.
Referring to fig. 6, the concentration sampler 100 further includes a host portion 40, and the host portion 40 includes the sampling pump 20, a control circuit 42, a touch display 43, a lithium battery 41, a flow sensor 44, and a driving circuit 45. The flow sensor 15 detects the flow rate of the main flow discharge port 115 to output a first flow detection signal. The flow sensor 44 is installed at the inlet of the sampling pump 20 to detect the flow rate of the inlet of the sampling pump 20 (the enrichment air path 21), thereby outputting a second flow detection signal. The control circuit 42 receives the first flow detection signal from the flow sensor 15 and controls the power of the fan 13 according to the first flow detection signal to adjust the flow rate of the main flow exhaust port 115. The control circuit 42 receives the second flow detection signal detected by the flow sensor 44, and controls the power of the sampling pump 20 according to the second flow detection signal to adjust the flow rate of the concentrating gas circuit 21. The flow rates in the vermilion flow rate exhaust port 115 and the concentration gas passage 21 are stabilized at the set flow rate values by the feedback control, and are maintained stable.
The control circuit 42 controls the operation of the sampling pump 20 via the drive circuit 45. The control circuit 42 also controls the current working state of the touch display screen 43, such as the first flow detection signal, the second flow detection signal, the rotation speed of the fan 13, the working state of the sampling pump 20, and the like.
The host part 40 further has a storage module, which can store the sampled sampling records, so as to facilitate data tracing. The host portion 40 also has a USB module connected to the control circuit 42, and the USB module is used for data interaction between the control circuit 42 and an external electronic device, and can export data stored in the memory module.
With continued reference to fig. 6, the lithium battery is a rechargeable lithium battery, and the host portion 40 further includes a charging interface 46 for charging the lithium battery. The main machine part 40 supplies power to the fan 13 through an interface 47, the housing 11 is embedded with an aviation interface 131 in a sealing manner, the aviation interface 131 is electrically connected with the fan 13, and in operation, the aviation interface 131 is electrically connected with the interface 47, so that the fan 13 is supplied with power through the interface 46 and the aviation interface 131. The air interface 131 is also electrically connected to the flow sensor 15, and the control circuit 42 further obtains a first flow detection signal of the flow sensor 15 through the interface 47 and the air interface 131.
Wherein the main flow exhaust port 115 exhausts gas through the main gas path. The flow rate of the fan 13 can reach about 300L/min (liter/min) under the windless condition, sufficient flow rate can be provided for the main gas circuit (the flow rate range is 0-150L/min under the general condition), and the fan 13 controls the rotating speed by receiving a PWM (pulse width modulation) signal from the control circuit, so that the flow rate control effect is achieved.
The flow sensor 15 is a digital gas flow sensor, the flow detection range is +/-250 slm (standard liter/min), the detection requirement of the range of 0-150 slm of the flow of the main gas path is met, the flow of the main gas path can be fed back in real time, and compared with an analog gas flow sensor, when data are transmitted for a long distance, the digital flow sensor is easy to use and stable in reading, and the measurement error is further reduced.
The above disclosure is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the scope of the present invention, therefore, the present invention is not limited by the appended claims.

Claims (10)

1. The utility model provides a concentrated sampling head of microorganism aerosol is gathered to cyclone formula which characterized in that includes:
the device comprises a shell, a valve body, a valve seat, a valve cover and a valve seat, wherein a small flow cavity and a main flow cavity which are isolated from each other, an air inlet communicated with the small flow cavity and the main flow cavity, a small flow exhaust communicated with the small flow cavity, and a main flow exhaust communicated with the main flow cavity are formed in the shell;
a virtual impact acceleration screen hole assembly, which sucks aerosol from the air inlet, screens the aerosol to obtain large-particle aerosol and small-particle aerosol, conveys the large-particle aerosol to the small flow cavity, and conveys the small-particle aerosol to the main flow cavity;
and the fan is arranged in the main flow cavity and accelerates the small particle aerosol to be sucked from the air inlet and discharged from the main flow exhaust port.
2. The concentrated sampling head according to claim 1, wherein the virtual impact acceleration sieve hole assembly comprises an aerosol particle acceleration plate and an aerosol particle size separation plate, the aerosol particle acceleration plate is installed at the air inlet, a plurality of first sieve holes are opened on the aerosol particle acceleration plate at positions communicated with the small flow cavities, the aerosol particle size separation plate is installed at an inner side of the aerosol particle acceleration plate at a certain interval and forms a flow cavity with the aerosol particle acceleration plate, a second sieve hole is opened on the aerosol particle size separation plate at a position opposite to the first sieve hole, a main vent hole is opened on the aerosol particle size separation plate at a position opposite to the main flow cavity, and the diameter of the second sieve hole is larger than that of the first sieve hole.
3. The concentrated sampling head according to claim 1, wherein an accelerating flow guide is formed in the small flow chamber and gradually downward, the small flow exhaust port is located at the lowest point of the accelerating flow guide, and the accelerating flow guide accelerates the large particle aerosol and discharges the large particle aerosol from the small flow exhaust port.
4. The concentrated sampling head according to claim 3, wherein the small flow chamber is disposed around the main flow chamber and the acceleration flow leader is an arcuate flow leader that surrounds the main flow chamber.
5. The concentrate sampling head of claim 4, wherein there are two of said flow leaders, and wherein said flow leaders extend from both sides around said main flow chamber and meet at their ends.
6. A thickening sampling head according to claim 1, wherein said housing is formed with a gradually inwardly converging flare outside of said virtual impaction acceleration screen assembly to form a converging zone outside of said virtual impaction acceleration screen assembly.
7. The thickening and sampling head of claim 1, further comprising a flow sensor disposed at said primary flow vent, said blower being an adjustable flow blower.
8. A concentrated sampler for collecting microbial aerosol in a cyclone manner, which is characterized by comprising a concentrated sampling head, a sampling pump and a sampling bottle, wherein the concentrated sampling head is as claimed in any one of claims 1 to 7, the sampling pump is communicated with the small-flow exhaust port through a concentrated gas path to extract large-particle aerosol, and the sampling bottle is installed on the concentrated gas path and collects the large-particle aerosol.
9. The concentrate sampler of claim 8, further comprising a lithium battery that powers the fan and the sampling pump.
10. The concentrate sampler of claim 8, wherein the concentrate gas circuit has a flow sensor disposed thereon, and the sampling pump is a variable flow pump.
CN202110803424.1A 2021-07-15 2021-07-15 Concentrated sampling head and concentrated sample thief of microorganism aerosol are gathered to cyclone Pending CN113567194A (en)

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CN114769005A (en) * 2022-04-12 2022-07-22 北京纳颗环境科技有限公司 Aerosol gas distribution device of electric mobility classifier
CN117516996A (en) * 2023-11-07 2024-02-06 重庆交通大学 Separation device and detection system for large-flow submicron particulate aerosol

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CN114769005A (en) * 2022-04-12 2022-07-22 北京纳颗环境科技有限公司 Aerosol gas distribution device of electric mobility classifier
CN114769005B (en) * 2022-04-12 2024-02-20 北京纳颗环境科技有限公司 Gas-soluble rubberized fabric gas device of electric mobility classifier
CN117516996A (en) * 2023-11-07 2024-02-06 重庆交通大学 Separation device and detection system for large-flow submicron particulate aerosol
CN117516996B (en) * 2023-11-07 2024-05-28 重庆交通大学 Separation device and detection system for large-flow submicron particulate aerosol

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