CN113544275A - 展示与分泌目的多肽的酵母展示***及其用途 - Google Patents
展示与分泌目的多肽的酵母展示***及其用途 Download PDFInfo
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Abstract
本发明涉及能够在细胞表面展示目的多肽并能够分泌目的多肽至培养基中的酵母展示***、以及所述酵母展示***的用途。本发明的酵母展示***能够稳定地构建108数量级的目的多肽变体展示文库,其中在细胞表面展示的方式使得能够借助高通量筛选选择特定的目的多肽变体,分泌至培养基中的方式使得能够进行目的多肽变体的生物化学表征;特别地,本发明的酵母展示***能够用于构建抗体的变体展示文库,从而获得高亲和力的抗体变体。
Description
PCT国内申请,说明书已公开。
Claims (11)
- PCT国内申请,权利要求书已公开。
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CN112646833A (zh) * | 2020-11-05 | 2021-04-13 | 杭州阿诺生物医药科技有限公司 | 一种全人源抗体酵母展示技术的设计与构建 |
CN115247136A (zh) * | 2021-04-29 | 2022-10-28 | 上海国龙生物科技有限公司 | 一种制备表面展示果糖基转移酶的毕赤酵母的方法 |
CN114717254B (zh) * | 2022-04-29 | 2024-03-12 | 深圳大学 | 酵母工程菌展示表达乳酸链球菌素Nisin的方法及其应用 |
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CN104870471A (zh) * | 2013-01-03 | 2015-08-26 | 默克专利股份公司 | 通过在酿酒酵母中的表达产生可分泌抗体的方法 |
CN106397598A (zh) * | 2016-02-23 | 2017-02-15 | 上海交通大学 | 多价多特异性抗体及免疫杂合蛋白的表达和制备方法 |
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CN107108750A (zh) * | 2014-10-28 | 2017-08-29 | 默克专利股份公司 | 细胞表面上非共价含Fc结构域的蛋白质展示的方法 |
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CA2489595C (en) * | 2002-06-14 | 2013-05-28 | Simon E. Hufton | Recombination of nucleic acid library members |
PL3241842T3 (pl) * | 2007-06-26 | 2024-06-10 | F-Star Therapeutics Limited | Prezentacja środków wiążących |
ES2410579T5 (es) * | 2008-12-16 | 2021-10-04 | Novartis Ag | Sistemas de despliegue de levaduras |
RU2625033C2 (ru) * | 2011-12-22 | 2017-07-11 | Ф. Хоффманн-Ля Рош Аг | Система индикации на основе полноразмерного антитела для эукариотических клеток и ее применение |
KR20150013503A (ko) * | 2012-05-11 | 2015-02-05 | 머크 샤프 앤드 돔 코포레이션 | 표면 앵커링된 경쇄 미끼 항체 디스플레이 시스템 |
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CN104870471A (zh) * | 2013-01-03 | 2015-08-26 | 默克专利股份公司 | 通过在酿酒酵母中的表达产生可分泌抗体的方法 |
US20150337292A1 (en) * | 2013-01-03 | 2015-11-26 | Merck Patent Gmbh | Method of producing secretable antibodies by expression in saccharomyces cerevisiae |
CN107108750A (zh) * | 2014-10-28 | 2017-08-29 | 默克专利股份公司 | 细胞表面上非共价含Fc结构域的蛋白质展示的方法 |
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CN106397598A (zh) * | 2016-02-23 | 2017-02-15 | 上海交通大学 | 多价多特异性抗体及免疫杂合蛋白的表达和制备方法 |
CN106397599A (zh) * | 2016-02-23 | 2017-02-15 | 上海交通大学 | 二价双特异性抗体杂交蛋白的表达和制备方法 |
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Application publication date: 20211022 |