CN113533204B - Electric-control observation system for capturing micro-fluid bubbles - Google Patents
Electric-control observation system for capturing micro-fluid bubbles Download PDFInfo
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Abstract
An electric control observation system for capturing microfluid vapor bubbles is characterized in that a single photon counter, a high-speed camera, a picosecond pulse laser and a high-frequency voltage source are connected to a trigger synchronous generator; the microfluidic viewing assembly includes a cap layer, a lead-in layer, and a substrate; a micro-channel observation area is arranged on the surface of the cover layer; the leading-in layer is positioned between the cover layer and the substrate, a liquid leading-in port is formed at the front end of the leading-in layer, a liquid extraction port is formed at the rear end of the leading-in layer, a picosecond pulse laser leading-in port is formed at the left end of the leading-in layer, and a picosecond pulse laser leading-out port is formed at the right end of the leading-in layer; a metal electrode is deposited in the groove of the substrate; the picosecond pulse laser irradiates bubbles generated by the microfluid observation component, bubble scattered light strikes the beam splitter prism, the single photon counter captures the light split of the beam splitter prism for microscopic observation, and the high-speed camera captures the light split of the beam splitter prism for auxiliary observation. The invention is beneficial to regulating and controlling bubbles in the microfluid and improving the accuracy and stability of the microfluid experiment.
Description
Technical Field
The invention relates to the technical field of microfluidics, in particular to an electric control observation system for capturing microfluid vapor bubbles.
Background
Microfluidics is an emerging interdiscipline involving chemistry, fluid physics, microelectronics, new materials, biology, and biomedical engineering, using microchannels (tens to hundreds of microns in size) to process or manipulate tiny fluids (nanoliters to attoliters in volume). Because of the miniaturization, integration, and other features, microfluidic devices are often referred to as microfluidic chips, also referred to as lab-on-a-chip and micro total analysis systems. One of the important features of microfluidics is the unique fluid properties in microscale environments, such as laminar flow and droplets. With these unique fluidic phenomena, microfluidics can achieve microfabrication and micromanipulation that are difficult to accomplish with conventional methods.
In microfluid, bubble formation first forms a bubble core, and the bubble formation must have two conditions, namely, supersaturated gas in liquid and energy required for nucleation. Bubble nucleation also occurs in both spontaneous and non-spontaneous nucleation modes, and if a bubble core is formed, the bubble expands against the pressure of the liquid, and on the other hand, because new phase formation increases the surface energy, sufficient energy must be provided to form a bubble core with critical dimensions in the liquid.
In microfluidic technology, the generated bubbles can have a great influence on applications, such as applications requiring strict quantification of liquids or chromatographic analysis. Bubbles in liquid samples are a problem often found in microfluidic experiments and they are often difficult to eliminate from the sample. How to capture, observe and regulate bubbles generated by microfluid so as to improve the accuracy and stability of a microfluid experiment is a technical problem to be solved urgently.
Disclosure of Invention
Therefore, the invention provides an electric regulation and control observation system for capturing micro-fluid bubbles, which can be used for capturing, observing, regulating and controlling the bubbles generated by micro-fluid and solving the problems of low accuracy and poor stability caused by the generation of the bubbles in a micro-fluid experiment.
In order to achieve the above purpose, the invention provides the following technical scheme: an electric-regulation observation system for capturing microfluid vapor bubbles comprises a single photon counter, a high-speed camera, a picosecond pulse laser, a high-frequency voltage source, a trigger synchronous generator, a beam splitter prism and a microfluid observation assembly; the single photon counter, the high-speed camera, the picosecond pulse laser and the high-frequency voltage source are connected to one trigger synchronous generator;
the microfluidic viewing assembly includes a cap layer, an introduction layer, and a substrate; a micro-channel observation area is arranged on the surface of the cover layer; the leading-in layer is positioned between the cover layer and the substrate, a liquid leading-in port is formed at the front end of the leading-in layer, a liquid extracting port is formed at the rear end of the leading-in layer, a picosecond pulse laser leading-in port is formed at the left end of the leading-in layer, and a picosecond pulse laser leading-out port is formed at the right end of the leading-in layer; a groove is etched in the upper end of the substrate, and a metal electrode is deposited in the groove;
the picosecond pulse laser generates picosecond pulse laser to irradiate bubbles generated by the microfluid observation component, bubble scattering light is irradiated on the beam splitter prism, the single photon counter captures the light split of the beam splitter prism for microscopic observation, and the high-speed camera captures the light split of the beam splitter prism for auxiliary observation;
the high-frequency voltage source is electrically connected with the microfluid observation assembly, and the high-frequency voltage source provides an electric field for the microfluid observation assembly.
The optimal scheme of the electric-control observation system for capturing the microfluid vapor bubbles further comprises a data acquisition unit and an observation computer, wherein the single-photon counter and the high-speed camera are electrically connected with the data acquisition unit, the observation computer is electrically connected with the data acquisition unit, and the observation computer processes and displays the data of the single-photon counter and the high-speed camera acquired by the data acquisition unit.
As a preferred scheme of the electric-control observation system for capturing the microfluid vapor bubbles, the observation computer couples microscopic observation information acquired by the single photon counter with auxiliary observation information acquired by the high-speed camera through a three-dimensional reconstruction algorithm, and the observation computer displays the coupled bubble three-dimensional imaging in real time.
As a preferred scheme of the electric-regulation observation system for capturing the microfluid vapor bubbles, a micro-channel is formed on the inner side of the introduction layer and is communicated with the liquid introduction port, the liquid extraction port, the picosecond pulse laser introduction port and the picosecond pulse laser extraction port.
As a preferred scheme of the electric-regulation observation system for capturing the microfluid vapor bubbles, the micro-channel is positioned above the metal electrode in the groove and is communicated with the groove.
As a preferred scheme of the electric-control observation system for capturing the micro-fluid bubbles, the metal electrodes are at least provided with two rows and three rows, and the height of the metal electrodes is smaller than the depth of the grooves.
The optimal scheme of the electric regulation observation system for capturing the microfluid vapor bubbles further comprises a liquid storage tank, a liquid phase pump, a subcooler, a needle valve, a condenser and a collecting tank; the liquid storage tank is connected with the liquid phase pump through an input pipeline, the liquid phase pump is connected with the subcooler through an input pipeline, the subcooler is connected with the needle valve through an input pipeline, and the needle valve is connected with the liquid inlet through an input pipeline;
the liquid extraction port is connected with the collecting tank through an output pipeline, the collecting tank is connected with the condenser through an output pipeline, and the condenser is connected with the liquid storage tank through an output pipeline.
As a preferred scheme of the electric-regulation observation system for capturing the micro-fluid bubbles, the high-frequency voltage source provides periodically conducted voltage for the metal electrode deposited in the groove.
As a preferred scheme of the electric-control observation system for capturing the microfluidic vapor bubbles, the bubbles generated by the microfluidic observation assembly move to a region with weak electric field gradient, and the fluid in the microfluidic observation assembly moves to a region with strong electric field gradient.
As a preferred scheme of the electric-regulation observation system for capturing the microfluid vapor bubbles, the wavelength of the picosecond pulse laser is 532 nm; the single photon counter counts by converting incident photons into TTL pulses.
The invention has the following advantages: the device is provided with a single photon counter, a high-speed camera, a picosecond pulse laser, a high-frequency voltage source, a trigger synchronous generator, a beam splitter prism and a microfluid observation assembly; the single photon counter, the high-speed camera, the picosecond pulse laser and the high-frequency voltage source are connected to a trigger synchronous generator; the microfluidic viewing assembly includes a cap layer, a lead-in layer, and a substrate; a micro-channel observation area is arranged on the surface of the cover layer; the leading-in layer is positioned between the cover layer and the substrate, a liquid leading-in port is formed at the front end of the leading-in layer, a liquid extraction port is formed at the rear end of the leading-in layer, a picosecond pulse laser leading-in port is formed at the left end of the leading-in layer, and a picosecond pulse laser leading-out port is formed at the right end of the leading-in layer; a groove is etched at the upper end of the substrate, and a metal electrode is deposited in the groove; the picosecond pulse laser device generates picosecond pulse laser to irradiate bubbles generated by the microfluid observation assembly, bubble scattering light is irradiated on the beam splitter prism, the single photon counter captures the light split of the beam splitter prism for microscopic observation, and the high-speed camera captures the light split of the beam splitter prism for auxiliary observation; the high-frequency voltage source is electrically connected with the microfluid observation assembly and provides an electric field for the microfluid observation assembly. The invention can realize the combined observation of the macro and micro of the micro-fluid bubbles, is beneficial to regulating and controlling the bubbles in the micro-fluid and improves the accuracy and the stability of the micro-fluid experiment.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.
The structures, ratios, sizes, and the like shown in the present specification are only used for matching with the contents disclosed in the specification, so that those skilled in the art can understand and read the present invention, and do not limit the conditions for implementing the present invention, so that the present invention has no technical significance, and any structural modifications, changes in the ratio relationship, or adjustments of the sizes, without affecting the functions and purposes of the present invention, should still fall within the scope of the present invention.
FIG. 1 is a schematic diagram of an electrical tuning observation system for capturing micro-fluid vapor bubbles provided in an embodiment of the present invention;
fig. 2 is a schematic top view of a microfluidic observation assembly in the electrically-controlled observation system for capturing a microfluidic vapor bubble according to the embodiment of the present invention;
fig. 3 is a schematic perspective view of a microfluidic observation assembly in the electrical-controlled observation system for capturing microfluidic vapor bubbles according to the embodiment of the present invention;
FIG. 4 is a schematic voltage diagram of an electrical tuning observation system for capturing micro-fluid bubbles according to an embodiment of the present disclosure;
fig. 5 is a schematic diagram of movement of bubbles in an electric field in the electric-tuning observation system for capturing micro-fluid bubbles provided in the embodiment of the present invention.
In the figure, 1, a single photon counter; 2. a high-speed camera; 3. a picosecond pulse laser; 4. a high frequency voltage source; 5. triggering a synchronous generator; 6. a beam splitter prism; 7. a microfluidic viewing assembly; 8. a cap layer; 9. an introduction layer; 10. a substrate; 11. a liquid introduction port; 12. a liquid extraction port; 13. a picosecond pulsed laser introduction port; 14. a picosecond pulse laser leading-out port; 15. a groove; 16. a metal electrode; 17. a data acquisition unit; 18. an observation computer; 19. a micro flow channel; 20. a liquid storage tank; 21. a liquid phase pump; 22. a subcooler; 23. a needle valve; 24. a condenser; 25. and (4) collecting the tank.
Detailed Description
The present invention is described in terms of particular embodiments, other advantages and features of the invention will become apparent to those skilled in the art from the following disclosure, and it is to be understood that the described embodiments are merely exemplary of the invention and that it is not intended to limit the invention to the particular embodiments disclosed. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1, 2 and 3, an embodiment of the present invention provides an electrical modulation observation system for capturing a microfluidic vapor bubble, including a single photon counter 1, a high-speed camera 2, a picosecond pulse laser 3, a high-frequency voltage source 4, a trigger synchronous generator 5, a beam splitter prism 6 and a microfluidic observation component 7; the single photon counter 1, the high-speed camera 2, the picosecond pulse laser 3 and the high-frequency voltage source 4 are connected to one trigger synchronous generator 5;
the microfluidic viewing assembly 7 comprises a cover layer 8, an introduction layer 9 and a substrate 10; the surface of the cover layer 8 is provided with a micro-channel 19 observation area; the lead-in layer 9 is positioned between the cover layer 8 and the substrate 10, a liquid lead-in port 11 is formed at the front end of the lead-in layer 9, a liquid extraction port 12 is formed at the rear end of the lead-in layer 9, a picosecond pulse laser lead-in port 13 is formed at the left end of the lead-in layer 9, and a picosecond pulse laser lead-out port 14 is formed at the right end of the lead-in layer 9; a groove 15 is etched in the upper end of the substrate 10, and a metal electrode 16 is deposited in the groove 15;
the picosecond pulse laser 3 generates picosecond pulse laser to irradiate bubbles generated by the microfluidic observation component 7, bubble scattering light impinges on the beam splitter prism 6, the single photon counter 1 captures light split of the beam splitter prism 6 for microscopic observation, and the high-speed camera 2 captures light split of the beam splitter prism 6 for auxiliary observation;
the high-frequency voltage source 4 is electrically connected to the microfluidic observing assembly 7, and the high-frequency voltage source 4 supplies an electric field to the microfluidic observing assembly 7.
In this embodiment, the picosecond pulse laser 3 has a wavelength of 532nm, and the picosecond pulse laser 3 has a pulse width of picoseconds. The micro-fluid observation assembly has the characteristics of picosecond-level ultrashort pulse width, adjustable repetition frequency, high pulse energy and the like, and 532nm picosecond pulse laser generated by the picosecond pulse laser 3 is applied to the nucleation vapor bubble of the micro-fluid observation assembly 7.
In this embodiment, the photon counter counts by converting incident photons into TTL pulses. In particular, an incident photon can be converted to a TTL pulse (<30ps) by the SPCM-AQRH single photon detector. SPCM-AQRH has good dynamic range and single photon detection capability in the wave band of 400-1060nm, and the peak photon detection efficiency is higher than 70% and is provided in the active area with the diameter of 700nm and the diameter of 180 mu m by utilizing a unique silicon avalanche photodiode and a circular active area. The photodiode is used for thermoelectric refrigeration and temperature control, and stable performance is guaranteed under the condition of environmental temperature change. Also provides over-illumination protection from ambient light.
In this embodiment, the system further includes a data acquisition unit 17 and an observation computer 18, the single photon counter 1 and the high-speed camera 2 are both electrically connected to the data acquisition unit 17, the observation computer 18 is electrically connected to the data acquisition unit 17, and the observation computer 18 processes and displays data of the single photon counter 1 and the high-speed camera 2 acquired by the data acquisition unit 17. The observation computer 18 couples the microscopic observation information acquired by the single photon counter 1 with the auxiliary observation information acquired by the high-speed camera 2 through a three-dimensional reconstruction algorithm, and the observation computer 18 displays the coupled bubble three-dimensional imaging in real time.
Specifically, a three-dimensional reconstruction algorithm is available, for example, an existing optical fiber-PIC chip coupling scene three-dimensional image reconstruction signal preprocessing algorithm provides reliable data for 3D image reconstruction by constructing a spatial average denoising filter model and a Sobel edge sharpening operator. The bubble image three-dimensional reconstruction in the embodiment may adopt a corresponding coupled scene three-dimensional image reconstruction signal preprocessing algorithm.
In this embodiment, a micro flow channel 19 is formed inside the introduction layer 9, and the micro flow channel 19 communicates with the liquid introduction port 11, the liquid extraction port 12, the picosecond pulse laser introduction port 13, and the picosecond pulse laser extraction port 14. The micro-channel 19 is positioned above the metal electrode 16 in the groove 15, and the micro-channel 19 is communicated with the groove 15. The metal electrode 16 has at least two rows and three columns, and the height of the metal electrode 16 is smaller than the depth of the groove 15.
Specifically, the microfluidic observation assembly 7 is divided into three layers, the first layer, the second layer and the third layer from top to bottom are respectively a cover layer 8, a laser and fluid introduction layer 9 and a substrate 10, the cover layer 8 divides an observation region, and the observation region is above one metal electrode 16 and is convenient to capture by the metal electrode 16. On the substrate 10 of the third layer, the grooves 15 are etched, and then the metal electrodes 16 of 2 rows and 2 columns are deposited in the grooves 15, wherein the height of the metal electrodes 16 is lower than the depth of the grooves 15, so that bubbles in the microfluid can be captured.
In this embodiment, the system further comprises a liquid storage tank 20, a liquid phase pump 21, a subcooler 22, a needle valve 23, a condenser 24 and a collecting tank 25; the liquid storage tank 20 is connected with the liquid phase pump 21 through an input pipeline, the liquid phase pump 21 is connected with the subcooler 22 through an input pipeline, the subcooler 22 is connected with the needle valve 23 through an input pipeline, and the needle valve 23 is connected with the liquid inlet 11 through an input pipeline; the liquid extraction port 12 is connected with the collecting tank 25 through an output pipeline, the collecting tank 25 is connected with the condenser 24 through an output pipeline, and the condenser 24 is connected with the liquid storage tank 20 through an output pipeline.
Specifically, the liquid in the liquid storage tank 20 is transferred to the subcooler 22 through the liquid phase pump 21 for heat exchange treatment, and then is input to the microfluidic observation assembly 7 through the liquid introduction port 11, and the needle valve 23 can be used for controlling the on-off of the fluid. The liquid discharged from the liquid discharge port 12 of the microfluidic observing module 7 is collected by the collecting tank 25, and then condensed by the condenser 24 and transferred to the reservoir 20 for use.
In this embodiment, the high-frequency voltage source 4 provides a periodically conducting voltage to the metal electrode 16 deposited inside the groove 15. The bubbles generated in the microfluidic observation device 7 move to a region where the electric field gradient is weak, and the fluid in the microfluidic observation device 7 moves to a region where the electric field gradient is strong.
Referring to fig. 4, in order to provide the working principle of the ac voltage supplied by the high-frequency voltage source 4, in fig. 5, two metal electrodes 16 in each row are paired and are each numbered, and the voltage turn-on period is 500ps, in the 500ps, one metal electrode 16 in each 100ps is turned on, and the other metal electrodes 16 in each Number are all turned off.
Referring to fig. 5, the particles in the non-uniform electric field move due to polarization effect, which is called Dielectrophoresis (DEP), and according to the DEP principle, the bubbles are polarized and then move to a place where the electric field gradient is weak, and the fluid moves to a place where the electric field gradient is strong. Therefore, the DEP is used for capturing the bubbles, and on one hand, the bubbles tend to move to the closed metal electrode 16, so that the bubbles move to the region to be observed a little bit by a little; on the other hand, the fluid tends to move in the direction of strong electric field strength, and the micro flow channel 19 of the fluid is strengthened, so that the substrate 10 is prevented from drying up.
The device is provided with a single photon counter 1, a high-speed camera 2, a picosecond pulse laser 3, a high-frequency voltage source 4, a trigger synchronous generator 5, a beam splitter prism 6 and a microfluid observation component 7; the single photon counter 1, the high-speed camera 2, the picosecond pulse laser 3 and the high-frequency voltage source 4 are connected to a trigger synchronous generator 5 together; the microfluidic viewing assembly 7 comprises a cover layer 8, an introduction layer 9 and a substrate 10; the surface of the cover layer 8 is provided with a micro-channel 19 observation area; the leading-in layer 9 is positioned between the cover layer 8 and the substrate 10, a liquid leading-in port 11 is formed at the front end of the leading-in layer 9, a liquid extraction port 12 is formed at the rear end of the leading-in layer 9, a picosecond pulse laser leading-in port 13 is formed at the left end of the leading-in layer 9, and a picosecond pulse laser leading-out port 14 is formed at the right end of the leading-in layer 9; a groove 15 is etched at the upper end of the substrate 10, and a metal electrode 16 is deposited in the groove 15; the picosecond pulse laser 3 generates picosecond pulse laser to irradiate bubbles generated by the microfluid observation component 7, bubble scattering light is irradiated on the beam splitter prism 6, the single photon counter 1 captures light split of the beam splitter prism 6 to carry out microscopic observation, and the high-speed camera 2 captures light split of the beam splitter prism 6 to carry out auxiliary observation; the high-frequency voltage source 4 is electrically connected to the microfluidic observing assembly 7, and the high-frequency voltage source 4 supplies an electric field to the microfluidic observing assembly 7. The invention connects the signal of the single photon counter 1, the high speed camera 2, the picosecond pulse laser and the high frequency voltage source 4 to the same trigger synchronous generator 5. Once a nucleation vapor bubble appears on the microfluid observation component 7, the vapor bubble can be rapidly moved and stabilized in an observation area due to the existence of electric regulation, 532nm picosecond pulse laser is irradiated on the nucleation vapor bubble of the platform, scattered light of the vapor bubble can be vertically irradiated on the beam splitter prism 6, beam splitting is realized on the beam splitter prism 6, one part of the scattered light is captured by the high-speed camera 2 so as to realize macroscopic auxiliary observation, the other part of the light is captured by the single photon counter 1 so as to realize microscopic observation, then, macroscopic and microscopic information is coupled by utilizing a three-dimensional reconstruction algorithm, and a real-time three-dimensional imaging is carried out on the captured vapor bubble at an observation computer end. The invention can realize the combined observation of the macro and micro of the micro-fluid bubbles, is beneficial to regulating and controlling the bubbles in the micro-fluid and improves the accuracy and the stability of the micro-fluid experiment.
Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (10)
1. An electric-regulation observation system for capturing microfluid vapor bubbles is characterized by comprising a single photon counter (1), a high-speed camera (2), a picosecond pulse laser (3), a high-frequency voltage source (4), a trigger synchronous generator (5), a beam splitter prism (6) and a microfluid observation assembly (7); the single photon counter (1), the high-speed camera (2), the picosecond pulse laser (3) and the high-frequency voltage source (4) are connected to one trigger synchronous generator (5) together;
the microfluidic viewing assembly (7) comprises a cover layer (8), an introduction layer (9) and a substrate (10); a micro-channel (19) observation area is arranged on the surface of the cover layer (8); the lead-in layer (9) is positioned between the cover layer (8) and the substrate (10), a liquid lead-in port (11) is formed at the front end of the lead-in layer (9), a liquid extraction port (12) is formed at the rear end of the lead-in layer (9), a picosecond pulse laser lead-in port (13) is formed at the left end of the lead-in layer (9), and a picosecond pulse laser lead-out port (14) is formed at the right end of the lead-in layer (9); a groove (15) is etched in the upper end of the substrate (10), and a metal electrode (16) is deposited in the groove (15);
the picosecond pulse laser (3) generates picosecond pulse laser to irradiate the vapor bubble generated by the microfluid observation component (7), the scattered light of the vapor bubble is irradiated on the beam splitter prism (6), the single photon counter (1) captures the light split of the beam splitter prism (6) for microscopic observation, and the high-speed camera (2) captures the light split of the beam splitter prism (6) for auxiliary observation;
the high-frequency voltage source (4) is electrically connected with the microfluid observation assembly (7), and the high-frequency voltage source (4) provides an electric field for the microfluid observation assembly (7).
2. The electric-controlled observation system for capturing micro-fluid bubbles according to claim 1, further comprising a data collector (17) and an observation computer (18), wherein the single-photon counter (1) and the high-speed camera (2) are both electrically connected with the data collector (17), the observation computer (18) is electrically connected with the data collector (17), and the observation computer (18) processes and displays data of the single-photon counter (1) and the high-speed camera (2) collected by the data collector (17).
3. The electrical controlled observation system for capturing micro-fluid bubbles according to claim 2, wherein the observation computer (18) couples the microscopic observation information collected by the single photon counter (1) and the auxiliary observation information collected by the high-speed camera (2) through a three-dimensional reconstruction algorithm, and the observation computer (18) displays the coupled three-dimensional imaging of the bubbles in real time.
4. The electric-controlled observation system for capturing micro-fluid vapor bubbles according to claim 1, characterized in that a micro-channel (19) is formed on the inner side of the introduction layer (9), and the micro-channel (19) is communicated with the liquid introduction port (11), the liquid extraction port (12), the picosecond pulse laser introduction port (13) and the picosecond pulse laser extraction port (14).
5. An electrically controlled viewing system for trapping micro-fluidic vapor bubbles according to claim 4, wherein the micro-channel (19) is located above the metal electrode (16) in the groove (15), the micro-channel (19) communicating with the groove (15).
6. An electrically controlled viewing system for trapping micro-fluidic vapor bubbles according to claim 5, wherein the metal electrodes (16) have at least two columns and three rows, and the height of the metal electrodes (16) is smaller than the depth of the grooves (15).
7. The electrically controlled observation system for capturing micro-fluidic vapor bubbles according to claim 4, further comprising a liquid storage tank (20), a liquid phase pump (21), a subcooler (22), a needle valve (23), a condenser (24) and a collection tank (25); the liquid storage tank (20) is connected with the liquid phase pump (21) through an input pipeline, the liquid phase pump (21) is connected with the subcooler (22) through an input pipeline, the subcooler (22) is connected with the needle valve (23) through an input pipeline, and the needle valve (23) is connected with the liquid inlet (11) through an input pipeline;
the liquid extraction port (12) is connected with the collecting tank (25) through an output pipeline, the collecting tank (25) is connected with the condenser (24) through an output pipeline, and the condenser (24) is connected with the liquid storage tank (20) through an output pipeline.
8. An electrically controlled viewing system for trapping micro-fluidic vapor bubbles according to claim 1, characterized in that said high-frequency voltage source (4) supplies a periodically conducting voltage to a metal electrode (16) deposited inside said recess (15).
9. The electrical modulation observation system for capturing micro-fluid bubbles according to claim 8, wherein the bubbles generated by the micro-fluid observation assembly (7) move to the region with weak electric field gradient, and the fluid inside the micro-fluid observation assembly (7) moves to the region with strong electric field gradient.
10. The electrically controlled observation system for trapping micro-fluidic vapor bubbles according to claim 1, characterized in that the picosecond pulse laser (3) has a wavelength of 532 nm; the single photon counter (1) counts by converting incident photons into TTL pulses.
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1741487A1 (en) * | 2005-07-05 | 2007-01-10 | ibidi GmbH | Microfluidic device for generating diffusion gradients and method therefor |
| CN101090766A (en) * | 2004-11-03 | 2007-12-19 | 维罗西股份有限公司 | Partial boiling in mini and micro-channels |
| CN103894248A (en) * | 2014-04-09 | 2014-07-02 | 国家纳米科学中心 | Micro-fluidic chip and micro-fluidic chip system for single cell analysis and single cell analyzing method |
| CN105973942A (en) * | 2016-05-10 | 2016-09-28 | 国家纳米科学中心 | Synchronous electric regulation and control and material transient absorption spectrum measuring device and method |
| CN107209096A (en) * | 2015-01-30 | 2017-09-26 | 惠普发展公司，有限责任合伙企业 | Fluid is aspirated and temperature adjustment |
| CN108387505A (en) * | 2018-02-06 | 2018-08-10 | 武汉大学 | A kind of multifunctional light tweezers system and method based on micro-fluidic chip |
| CN110354925A (en) * | 2019-08-21 | 2019-10-22 | 苏州大学 | It is a kind of comprising can deformation liquid metal electrode micro-fluidic chip and preparation method thereof |
| WO2020188563A1 (en) * | 2019-03-17 | 2020-09-24 | Technion Research & Development Foundation Limited | System and method for manipulating objects in a fluid |
| CN112557371A (en) * | 2020-12-08 | 2021-03-26 | 北京大学 | Nanoscopic multiphase interface transient observation device and method based on laser effect |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10944123B2 (en) * | 2017-09-25 | 2021-03-09 | University Of Wyoming | Electrical energy generation in fluidic channels and membranes using spontaneous capillary flow |
-
2021
- 2021-07-22 CN CN202110832547.8A patent/CN113533204B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101090766A (en) * | 2004-11-03 | 2007-12-19 | 维罗西股份有限公司 | Partial boiling in mini and micro-channels |
| EP1741487A1 (en) * | 2005-07-05 | 2007-01-10 | ibidi GmbH | Microfluidic device for generating diffusion gradients and method therefor |
| CN103894248A (en) * | 2014-04-09 | 2014-07-02 | 国家纳米科学中心 | Micro-fluidic chip and micro-fluidic chip system for single cell analysis and single cell analyzing method |
| CN107209096A (en) * | 2015-01-30 | 2017-09-26 | 惠普发展公司，有限责任合伙企业 | Fluid is aspirated and temperature adjustment |
| CN105973942A (en) * | 2016-05-10 | 2016-09-28 | 国家纳米科学中心 | Synchronous electric regulation and control and material transient absorption spectrum measuring device and method |
| CN108387505A (en) * | 2018-02-06 | 2018-08-10 | 武汉大学 | A kind of multifunctional light tweezers system and method based on micro-fluidic chip |
| WO2020188563A1 (en) * | 2019-03-17 | 2020-09-24 | Technion Research & Development Foundation Limited | System and method for manipulating objects in a fluid |
| CN110354925A (en) * | 2019-08-21 | 2019-10-22 | 苏州大学 | It is a kind of comprising can deformation liquid metal electrode micro-fluidic chip and preparation method thereof |
| CN112557371A (en) * | 2020-12-08 | 2021-03-26 | 北京大学 | Nanoscopic multiphase interface transient observation device and method based on laser effect |
Non-Patent Citations (6)
| Title |
|---|
| A multi-functional bubble-based microfluidic system;Khashayar Khoshmanesh et al;《Scientific Reports》;20150423;第5卷;全文 * |
| Bubble Control, Levitation, and Manipulation Using Dielectrophoresis;Carl V. Brown et al;《Advanced Materials Interfaces》;20201228;第8卷(第2期);全文 * |
| Experimental Study of Bubble Formation from a Micro-Tube in Non-Newtonian Fluid;Georgia Kontaxi et al;《Micromachines》;20210111;第12卷(第71期);全文 * |
| SiC Based Intergrated Micropillar Array Wick Thin Plate Heat Pipe Investigation;Zhenyu Wang et al;《20th International Conference on Electronic Packaging Technology》;20200604;全文 * |
| 基于Micro-PIV方法的微槽群热沉内流动与传热的可视化实验和理论研究;于东;《中国博士学位论文全文数据库》;20141031;第100-140页 * |
| 微槽群结构对微纳复合结构热沉表面汽泡动力学行为影响的可视化研究;戴新龙;《中国优秀硕士学位论文全文数据库》;20180331;全文 * |
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