CN113528599B - Production method of efficient chelating enzyme peptide - Google Patents
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Abstract
The invention belongs to the field of biological feeds, and relates to a production method of an efficient chelating ferment peptide. The method comprises the following steps: activating the strain for expanding culture to obtain fermentation seed liquid of bacillus subtilis, saccharomyces cerevisiae, lactobacillus plantarum and lactobacillus rhamnosus respectively; weighing bean dregs and bean pulp, and uniformly stirring to prepare a solid fermentation culture medium; inoculating bacillus subtilis to ferment seed liquid, adding secondary powder and adjusting water content to perform primary aerobic fermentation; inoculating Saccharomyces cerevisiae, lactobacillus plantarum, lactobacillus rhamnosus fermentation seed liquid, protease and inorganic mineral salt for secondary anaerobic fermentation, and obtaining the chelating ferment peptide after fermentation. The invention adopts the mode of synchronous aerobic and anaerobic step fermentation, bacterial enzyme synergistic fermentation, fermentation and chelation, has simple production process and high chelation rate of various mineral elements in the obtained chelation ferment peptideChelating Fe to Fe 2+ Mainly contains low phytic acid and high content of small peptide, organic acid and probiotics, and has remarkable economic benefit.
Description
Technical Field
The invention belongs to the field of biological feeds, and particularly relates to a production method of mineral element efficient chelating type ferment peptide suitable for poultry.
Background
Ca. The mineral elements such as Fe, cu, zn, mn relate to almost all life activity processes such as growth metabolism, signal transduction, immunoregulation, enzyme activation and the like of poultry such as laying hens, and have very important significance for maintaining normal physiological functions, health states and high-efficiency production performance of organisms. When the feed lacks corresponding mineral elements, the poultry can grow slowly, the disease resistance is reduced, the laying rate is reduced, the quality of the laid eggs is reduced, and the like.
Poultry mineral element supplementation has undergone four generations of development. First generation, inorganic salt type, such as: ferrous sulfate, copper sulfate, and the like. The inorganic salt type mineral elements have poor stability and low absorption and utilization rate, antagonism exists among different mineral elements, and the inorganic salt type mineral elements are easy to combine with phytic acid and the like to form insoluble salts to influence absorption. Second generation, organic acid type, such as: calcium lactate, zinc gluconate, etc. The absorption and utilization rate of the organic acid type mineral elements are improved compared with the first generation, but the problems of high price, poor stability, absorption competition among different mineral elements and the like still exist. Third generation, amino acid chelation, such as: ferrous glycinate, zinc methionine, and the like. The amino acid chelated mineral elements have high absorption and utilization rate, strong stability, safety and no toxic or side effect, but have extremely high price, and the use of the amino acid chelated mineral elements in large-scale cultivation greatly increases the cultivation cost. Fourth generation, small peptide chelated, such as: soybean peptide chelated calcium, casein chelated iron, and the like. The mineral elements are transported and absorbed in the form of chelates by means of the transport and absorption mechanism of small peptides. The small peptide chelated mineral element has the advantages of low price, high absorption and utilization rate, strong stability, no toxic or side effect and the like. The small peptide chelate also has biological functions of small peptide ligands, such as: enhancing immunity, resisting bacteria, resisting oxidation, and reducing cholesterol. However, the research of small peptide chelated mineral elements is in a theoretical stage, the actual industrial production is less, and the small peptide chelated mineral elements are difficult to purchase in the market.
In practical cultivation, a large amount of mineral elements such as Ca, fe, cu, zn, mn are often added to control the cultivation cost and to meet the requirements of poultry for mineral elements. The poultry has very low absorption and utilization rate of inorganic mineral salt, and most of the inorganic mineral salt is directly discharged out of the body, so that serious environmental pollution and resource waste are caused. The poultry ingests excessive inorganic mineral salts, and has great toxic and side effects on organisms. Furthermore, fe of inorganic salt type 2+ Extremely easy to oxidize into Fe 3+ Obviously reduce the Fe content of poultry 2+ Is absorbed and utilized to reduce body hemoglobin and reduce oxygen transportation capacity, thereby producing anemia.
At present, the supplementation of chelating mineral elements is the most ideal mineral element supplementation mode acknowledged. In the traditional production mode of the chelate mineral elements, proteins are hydrolyzed by chemical hydrolysis, enzymatic hydrolysis and other methods, then high-purity amino acids, small peptides and other small molecular substances are obtained by protein purification, or purer amino acids are obtained by an amino acid fermentation process or a chemical synthesis process, and then free mineral elements and the purified small molecular substances are chelated by corresponding chelation processes. Finally, the high-purity chelate mineral elements are obtained by washing with absolute ethyl alcohol, liquid chromatography separation and other methods. The obtained mineral element chelate has high purity and high stability. However, these high purity chelates are complicated in production process, high in equipment requirement and high in production cost, and cause difficulties in practical production and wide use of mineral element chelates. In addition, the single complex, which is sought after in a first place in high purity, is not necessarily the best way to supplement mineral elements. The single mineral element chelate or the single amino acid and small peptide chelate can not meet the comprehensive nutrition supplement of mineral elements and amino acids. In addition, the high-price amino acid and small peptide chelate greatly increases the cost of the culture feed, and is a great resistance to the development of the culture industry. All types of nutrients have their absorption pathways, but the absorption pathways are saturated. Although a single amino acid or small peptide chelate is much stronger than an inorganic mineral salt, the addition of an amount exceeding its absorption capacity also reduces the absorption of mineral elements.
Disclosure of Invention
Aiming at the problems of the existing mineral element supplementation of poultry, the invention provides a method for producing mineral element efficient chelating type ferment peptide by taking bean dregs and bean pulp as raw materials and performing synergistic fermentation chelating by using bacterial enzymes. The method has the advantages of simple production process, low cost and remarkable economic benefit. The obtained chelate ferment peptide has high chelation rate of various mineral elements, and chelated Fe is Fe 2+ Mainly, the phytic acid content is low, and the content of small peptide, organic acid, probiotics and the like is high.
In order to achieve the technical aim, the invention provides a method for producing mineral element efficient chelating ferment peptide by taking bean dregs and bean pulp as raw materials through fermentation and chelation, which comprises the following steps:
(1) Respectively carrying out activation and expansion culture on bacillus subtilis, saccharomyces cerevisiae, lactobacillus plantarum and lactobacillus rhamnosus to obtain corresponding fermentation seed liquid;
(2) Weighing bean dregs and bean pulp, and uniformly stirring to prepare a solid fermentation culture medium;
(3) Inoculating the bacillus subtilis fermentation seed liquid obtained in the step (1) into the solid fermentation culture medium prepared in the step (2), adding secondary powder, adding water according to the water content, uniformly stirring, and performing primary aerobic fermentation to obtain a fermentation culture medium for aerobic fermentation;
(4) And (3) inoculating the Saccharomyces cerevisiae fermentation seed liquid, the lactobacillus plantarum fermentation seed liquid, the lactobacillus rhamnosus fermentation seed liquid, the protease and the inorganic mineral salt obtained in the step (1) into the aerobic fermentation culture medium obtained in the step (3), uniformly stirring the mixture, sealing the mixture for secondary anaerobic fermentation, and obtaining the mineral element efficient chelating ferment peptide after the fermentation is finished.
Preferably, in the step (2), the bean dregs in the solid fermentation medium account for 25% -70% of the total mass, and the bean dregs account for 30% -75% of the total mass.
Preferably, the bacillus subtilis inoculation amount in the step (3) is 1-15% of the mass of the solid fermentation medium in the step (2); the adding amount of the secondary powder is 5-30% of the mass of the solid fermentation medium in the step (2).
Preferably, the water content in the step (3) is 25% -65%.
Preferably, the primary aerobic fermentation temperature in the step (3) is 20-40 ℃, and the aerobic fermentation time is 3-48 h.
Preferably, the seed liquid inoculation amount of the saccharomyces cerevisiae, the lactobacillus plantarum and the lactobacillus rhamnosus in the step (4) is 1-10% of the mass of the solid fermentation medium in the step (2).
Preferably, the proteases in step (4) are acid protease and papain; the total addition amount of the acid protease and the papain is 0.01% -2% of the mass of the solid fermentation medium in the step (2), the addition ratio of the acid protease to the papain is 1:1, and the enzyme activities are 6 ten thousand u/g.
Preferably, the inorganic mineral salt in step (4) is CaCO 3 、FeSO 4 ·7H 2 O、CuSO 4 ·5H 2 O、MnSO 4 ·H 2 O and ZnSO 4 ·7H 2 O。
Preferably, the CaCO in step (4) 3 The addition amount is 1 to 12 percent of the mass of the solid fermentation culture medium in the step (2); the FeSO 4 ·7H 2 The addition amount of O is 0.01 to 1.6 percent of the mass of the solid fermentation culture medium in the step (2); the CuSO 4 ·5H 2 The addition amount of O is 0.001-0.064% of the mass of the solid fermentation culture medium in the step (2); the MnSO 4 ·H 2 The addition amount of O is 0.01-1% of the mass of the solid fermentation culture medium in the step (2); the ZnSO is 4 ·7H 2 The addition amount of O is 0.01 to 1.2 percent of the mass of the solid fermentation medium in the step (2).
Preferably, the anaerobic fermentation temperature in the second stage in the step (4) is 20-40 ℃, and the fermentation time is 2-30 days.
Strain sources: lactobacillus plantarum (Lactobacillus plantarum, CGMCC 1.557) and lactobacillus rhamnosus (Lactobacillus rhamnose, CGMCC 1.2467) are purchased from China general microbiological culture collection center; bacillus subtilis (Bacillus subtilis) and Saccharomyces cerevisiae (Saccharomyces cerevisiae) were purchased from Biotech Inc.
Enzyme source: both acid protease and papain were purchased from Xia Chengmei biotechnology limited.
The beneficial effects of the invention are as follows:
(1) The fermentation and mineral element chelation are performed simultaneously, the fermentation process is simple and easy to operate, the production cost is low, the feeding cost of the mineral elements of the poultry is obviously reduced, and the requirements of large-scale production and application are met.
(2) The chelation rate of various mineral elements in the chelation type ferment peptide obtained by the invention is high, and the absorption and utilization rate of the mineral elements by poultry is obviously improved.
(3) The Fe in the chelate ferment peptide obtained by the invention is mainly Fe 2+ And chelate enzyme peptide pair Fe 2+ Has good antioxidation effect, and prolongs the service life of Fe 2+ The storage time of the poultry is obviously improved 2+ The absorption and utilization rate of the poultry feed additive reduces the occurrence of poultry anemia.
(4) According to the invention, the phytic acid content in the chelating ferment peptide is obviously reduced through the synergistic fermentation of the bacterial enzymes, the combination of phytic acid and mineral elements is avoided, and the absorption of the mineral elements is reduced.
(5) According to the invention, through the synergistic fermentation of the bacterial enzymes, the small peptide content, the organic acid content and the probiotics content in the chelating type ferment peptide are obviously increased, the nutrition quality of the chelating type ferment peptide is improved, the absorption of protein by poultry is facilitated, the health of intestinal flora is maintained, the immunity of the poultry is enhanced, and the use of antibiotics is reduced.
Drawings
FIG. 1 is a flow chart of the fermentation process of the present invention.
The specific embodiment is as follows:
the invention is further described below in connection with specific embodiments.
Example 1:
(1) Preparation of bacillus subtilis, saccharomyces cerevisiae, lactobacillus plantarum and lactobacillus rhamnosus by activation culture and fermentation seed liquid:
A. preparing a strain culture medium:
slant and plate medium: 5g of yeast extract, 10g of peptone, 10g of sodium chloride, 10g of glucose, 20g of agar and 1000mL of distilled water, and sterilizing for 30min at 121 ℃;
seed liquid culture medium: soybean 20g, brown sugar 20g and distilled water 1000mL, and sterilizing at 121deg.C for 30min;
B. activating various strains and preparing fermentation seed liquid:
preparation of bacillus subtilis activation and fermentation seed liquid: the bacillus subtilis strain stored at 4 ℃ is inoculated in a slant culture medium and cultured for 36 hours at 37 ℃. Then, single colonies were streaked on the plate and cultured for 36 hours at 37℃in an inverted manner, and the single colonies were selected and inoculated into a 250mL Erlenmeyer flask containing 50mL of the seed solution medium and cultured for 18 hours at 37℃at 160 r/min. The fermentation seed liquid is obtained by carrying out expansion culture with the inoculum size (v/v) of 3 percent according to the requirement, and the culture condition is that the temperature is 37 ℃ and the culture is 160r/min for 24 hours.
Activating saccharomyces cerevisiae and preparing fermentation seed liquid: saccharomyces cerevisiae strain stored at 4deg.C is inoculated into slant culture medium, and cultured at 28deg.C for 36 hr. Then, single colonies were streaked on the plate and cultured for 36 hours at 28℃in an inverted manner, and the single colonies were selected and inoculated into a 250mL Erlenmeyer flask containing 50mL of the seed solution medium and cultured for 18 hours at 28℃at 160 r/min. The fermentation seed liquid is obtained by carrying out expansion culture with the inoculum size (v/v) of 3 percent according to the requirement, and the culture condition is 28 ℃ and 160r/min for 24 hours.
Preparation of lactobacillus plantarum and lactobacillus rhamnosus activation and fermentation seed liquid: lactobacillus plantarum and lactobacillus rhamnosus strains stored at 4 ℃ are respectively inoculated in a slant culture medium and cultured for 48 hours at 37 ℃. Then, single colonies were streaked on the plate and cultured for 48 hours at 37℃in an inverted manner, and the single colonies were selected and inoculated into a 250mL anaerobic flask containing 230mL of the seed solution medium, and cultured for 18 hours at 37 ℃. The fermentation seed liquid is obtained by carrying out expansion culture according to the requirement with the inoculum size (v/v) of 3 percent, and the culture condition is that the culture is carried out for 24 hours at 37 ℃.
(2) Preparation of solid fermentation medium: the mass ratio of each component is as follows: bean dregs 50%, bean pulp 50%;
(3) Inoculating the prepared bacillus subtilis fermentation seed liquid into a fermentation medium according to the inoculation amount of 3% of the mass of the solid fermentation medium, adding secondary powder with the mass of 10% of the mass of the solid fermentation medium, adding water to ensure that the water content is 45%, uniformly stirring, performing primary aerobic fermentation, wherein the fermentation temperature is 32 ℃, and performing aerobic fermentation for 15 hours;
(4) Inoculating the prepared Saccharomyces cerevisiae, lactobacillus plantarum and lactobacillus rhamnosus fermentation seed liquid into the fermentation medium respectively according to the inoculation amount of 3% of the mass of the solid fermentation medium, adding protease with the mass of 0.5% of the mass of the solid fermentation medium (the ratio of acid protease to papain is 1:1), and finally adding CaCO with the mass of 6% 3 、0.8％FeSO 4 ·7H 2 O、0.032％CuSO 4 ·5H 2 O、0.5％MnSO 4 ·H 2 O、0.6％ZnSO 4 ·7H 2 And O, uniformly stirring, filling into a fermentation bag with a unidirectional exhaust hole, sealing for secondary anaerobic fermentation, wherein the fermentation temperature is 31 ℃, the fermentation time is 15 days, and obtaining the mineral element efficient chelating ferment peptide after the fermentation is finished.
Example 2:
(1) The preparation method of the activating and fermenting seed liquid of bacillus subtilis, saccharomyces cerevisiae, lactobacillus plantarum and lactobacillus rhamnosus is carried out in the same way as in example 1;
(2) Preparation of solid fermentation medium: the mass ratio of each component is as follows: 60% of bean dregs and 40% of bean pulp;
(3) Inoculating the prepared bacillus subtilis fermentation seed liquid into a fermentation medium according to the inoculation amount of 8% of the mass of the solid fermentation medium, adding secondary powder accounting for 24% of the mass of the solid fermentation medium, adding water to ensure that the water content is 55%, uniformly stirring, performing primary aerobic fermentation, wherein the fermentation temperature is 36 ℃, and performing aerobic fermentation for 24 hours;
(4) Inoculating the prepared Saccharomyces cerevisiae, lactobacillus plantarum and lactobacillus rhamnosus fermentation seed liquid into the fermentation medium respectively according to the inoculation amount of 10% of the mass of the solid fermentation medium, and adding 1.5% protease (the ratio of acid protease to papain is 1:1) and 12% CaCO (caco) of the mass of the solid fermentation medium 3 、0.4％FeSO 4 ·7H 2 O、0.05％CuSO 4 ·5H 2 O、0.1％MnSO 4 ·H 2 O、1.2％ZnSO 4 ·7H 2 And O, uniformly stirring, filling into a fermentation bag with a unidirectional exhaust hole, sealing for secondary anaerobic fermentation, wherein the fermentation temperature is 40 ℃, the fermentation time is 8 days, and obtaining the mineral element efficient chelating ferment peptide after the fermentation is finished.
Example 3:
(1) The preparation method of the activating and fermenting seed liquid of bacillus subtilis, saccharomyces cerevisiae, lactobacillus plantarum and lactobacillus rhamnosus is carried out in the same way as in example 1;
(2) Preparation of solid fermentation medium: the mass ratio of each component is as follows: 40% of bean dregs and 60% of bean pulp;
(3) Inoculating the prepared bacillus subtilis fermentation seed liquid into a fermentation medium according to the inoculation amount of 10% of the mass of the solid fermentation medium, adding secondary powder accounting for 18% of the mass of the solid fermentation medium, adding water to ensure that the water content is 40%, uniformly stirring, performing primary aerobic fermentation, wherein the fermentation temperature is 25 ℃, and performing aerobic fermentation for 48 hours;
(4) Inoculating the prepared Saccharomyces cerevisiae, lactobacillus plantarum and lactobacillus rhamnosus fermentation seed liquid into the fermentation medium respectively according to the inoculum size of 5% of the mass of the solid fermentation medium, adding 1% protease (the ratio of acid protease to papain is 1:1) of the mass of the solid fermentation medium, and finally adding 4% CaCO 3 、1.6％FeSO 4 ·7H 2 O、0.064％CuSO 4 ·5H 2 O、0.01％MnSO 4 ·H 2 O、0.2％ZnSO 4 ·7H 2 And O, uniformly stirring, filling into a fermentation bag with a unidirectional exhaust hole, sealing for secondary anaerobic fermentation, wherein the fermentation temperature is 37 ℃, the fermentation time is 2 days, and obtaining the mineral element efficient chelating ferment peptide after the fermentation is finished.
Example 4:
(1) The preparation method of the activating and fermenting seed liquid of bacillus subtilis, saccharomyces cerevisiae, lactobacillus plantarum and lactobacillus rhamnosus is carried out in the same way as in example 1;
(2) Preparation of solid fermentation medium: the mass ratio of each component is as follows: 25% of bean dregs and 75% of bean pulp.
(3) Inoculating the prepared bacillus subtilis fermentation seed liquid into a fermentation medium according to the inoculation amount of 1% of the mass of the solid fermentation medium, adding secondary powder accounting for 30% of the mass of the solid fermentation medium, adding water to ensure that the water content is 25%, uniformly stirring, performing primary aerobic fermentation, wherein the fermentation temperature is 40 ℃, and performing aerobic fermentation for 9 hours;
(4) Inoculating the prepared Saccharomyces cerevisiae, lactobacillus plantarum and lactobacillus rhamnosus fermentation seed liquid into a fermentation medium according to the inoculation amount of 8% of the mass of the solid fermentation medium, adding 2% protease (the ratio of acid protease to papain is 1:1) of the mass of the solid fermentation medium, and finally adding 8% CaCO 3 、0.01％FeSO 4 ·7H 2 O、0.01％CuSO 4 ·5H 2 O、0.8％MnSO 4 ·H 2 O、0.9％ZnSO 4 ·7H 2 O, stirring uniformly, sealing, carrying out secondary anaerobic fermentation at 20 ℃ for 21 days, and obtaining the mineral element efficient chelating ferment peptide after fermentation.
Example 5:
(1) The preparation method of the activating and fermenting seed liquid of bacillus subtilis, saccharomyces cerevisiae, lactobacillus plantarum and lactobacillus rhamnosus is carried out in the same way as in example 1;
(2) Preparation of solid fermentation medium: the mass ratio of each component is as follows: 70% of bean dregs and 30% of bean pulp;
(3) Inoculating the prepared bacillus subtilis fermentation seed liquid into a fermentation medium according to the inoculation amount of 15% of the mass of the solid fermentation medium, adding water to ensure that the water content is 65%, uniformly stirring, performing primary aerobic fermentation at 20 ℃ for 3 hours;
(4) Inoculating the prepared Saccharomyces cerevisiae, lactobacillus plantarum and lactobacillus rhamnosus fermentation seed liquid into the fermentation medium respectively according to the inoculation amount of 1% of the mass of the solid fermentation medium, adding protease with the mass of 0.01% of the mass of the solid fermentation medium (the ratio of acid protease to papain is 1:1), and finally adding CaCO with the mass of 1% 3 、1.2％FeSO 4 ·7H 2 O、0.001％CuSO 4 ·5H 2 O、1％MnSO 4 ·H 2 O、0.01％ZnSO 4 ·7H 2 O, stirring uniformly, sealing, carrying out secondary anaerobic fermentation at the fermentation temperature of 26 ℃ for 30 days, and obtaining the mineral element efficient chelating ferment peptide after the fermentation is finished.
Table 1 shows the chelation rates of mineral elements in the chelation type enzyme peptides of the examples
| Mineral elements | Example 1 | Example 2 | Example 3 | Example 4 | Example 5 |
| Ca(％) | 89.87 | 82.35 | 85.74 | 87.86 | 93.14 |
| Fe(％) | 97.73 | 98.16 | 97.13 | 98.79 | 97.38 |
| Cu(％) | 76.42 | 74.24 | 73.85 | 77.43 | 78.29 |
| Mn(％) | 96.84 | 96.92 | 97.46 | 96.25 | 96.18 |
| Zn(％) | 96.28 | 95.63 | 96.37 | 95.82 | 97.93 |
Table 2 shows the content of the related substances in the chelate-type enzyme peptide in each example
Example 6: feeding experiment
The experimental site was a hen farm from Zhejiang Kangxing biotechnology Co., ltd, and the high-efficiency chelating enzyme peptide used was prepared as in example 1. The 72 local three-yellow laying hens are randomly combined into two groups, 36 laying hens in each group are fed for 60 days according to the feed formula shown in table 3, and the experimental results are shown in tables 4, 5 and 6.
As shown in the results of tables 4 to 6, the amount of the highly efficient chelating enzyme peptide of the present invention was 5%. The feeding of the chelating enzyme peptide obviously improves the average daily feed intake, the laying rate, the average egg weight and the eggshell thickness of the laying hens, reduces the soft breaking rate, and shows that the chelating enzyme peptide obtained by the invention improves the production performance of the laying hens. Meanwhile, the content of Ca, fe, cu, mn, zn in the excrement of the laying hens is obviously reduced by feeding the chelating enzyme peptide, which shows that the chelating enzyme peptide has the effects of improving the absorption of mineral elements by the laying hens and reducing the discharge of the mineral elements along with the excrement. The red blood cells, the haemoglobin and the hematocrit of the red blood cells fed with the chelating enzyme peptide are all obviously higher than those of a control group, which indicates that the feeding of the chelating enzyme peptide improves the Fe content of the high-strength layer chicken 2+ 、Cu 2+ And the like, enhances the transportation of oxygen in the layers, and avoids the occurrence of layer anemia.
Table 3 shows the formula of the feed for feeding experimental layer chicken
| Composition of the composition | Experimental group (%) | Control (%) |
| Corn | 62 | 62 |
| Bean pulp | 26.28 | 26.28 |
| Multi-element vitamin | 0.05 | 0.05 |
| DL methionine | 0.12 | 0.12 |
| Stone | 5 | 5 |
| Dibasic calcium phosphate | 1.4 | 1.4 |
| Vc | 0.1 | 0.1 |
| Phytase enzyme | 0.05 | 0.05 |
| Mineral elements | 5 (chelating ferment peptide) | 5 (unfermented feed) |
| Totalizing | 100 | 100 |
Note that: the unfermented feed added correspondingly in the mineral elements of the control group is different from the chelating ferment peptide added in the experimental group in that the control group is not added with strains and protease, is not fermented, and the other added substances and the dosage are the same as those of the experimental group.
Table 4 shows the results of the test of the productivity of the feeding test layer
Table 5 shows the mineral ion content of the excrement of the laying hens fed in the experiment
| Mineral elements | Experimental group | Control group |
| Ca | 18.99 | 25.8 |
| Fe | 0.44 | 0.70 |
| Cu | 0.059 | 0.078 |
| Mn | 0.18 | 0.26 |
| Zn | 0.15 | 0.21 |
Table 6 shows the conventional test results of the blood of the layer fed in the experiment
| Mineral elements | Experimental group | Control group |
| White blood cells | 2.48 | 2.19 |
| Erythrocyte cell | 3.52 | 2.24 |
| Hemoglobin (hemoglobin) | 179 | 137 |
| Packed red blood cell | 43.8 | 30.6 |
Description: the above embodiments are only for illustrating the present invention and not for limiting the technical solution described in the present invention; thus, while the invention has been described in detail with reference to the various embodiments described above, it will be understood by those skilled in the art that the invention may be modified or equivalents; all technical solutions and modifications thereof that do not depart from the spirit and scope of the present invention are intended to be included in the scope of the appended claims.
Claims (4)
1. Use of a highly effective chelating ferment peptide for raising poultry to increase mineral element absorption and laying rate of poultry, characterized in that the Gao Xiaoao ferment peptide is produced by the following steps:
(1) Respectively carrying out activation and expansion culture on bacillus subtilis, saccharomyces cerevisiae, lactobacillus plantarum and lactobacillus rhamnosus to obtain corresponding fermentation seed liquid;
(2) Weighing bean dregs and bean pulp, and uniformly stirring to prepare a solid fermentation culture medium; wherein, the bean dregs in the solid fermentation medium account for 25 to 70 percent of the total mass, and the bean pulp accounts for 30 to 75 percent of the total mass;
(3) Inoculating the bacillus subtilis fermentation seed liquid obtained in the step (1) into the solid fermentation medium prepared in the step (2), wherein the bacillus subtilis inoculation amount is 1% -15% of the mass of the solid fermentation medium in the step (2); then adding secondary powder, adding water according to the water content, uniformly stirring, and performing primary aerobic fermentation to obtain a fermentation medium for aerobic fermentation; the primary aerobic fermentation temperature is 20-40 ℃, and the aerobic fermentation time is 3-48 hours;
(4) Inoculating the Saccharomyces cerevisiae fermentation seed liquid, the lactobacillus plantarum fermentation seed liquid, the lactobacillus rhamnosus fermentation seed liquid, the protease and the inorganic mineral salt obtained in the step (1) into the aerobic fermentation culture medium obtained in the step (3), uniformly stirring, sealing, performing secondary anaerobic fermentation at the fermentation temperature of 20-40 ℃ for 2-30 days; obtaining high-efficiency chelating ferment peptide after fermentation; the seed liquid inoculation amount of the saccharomyces cerevisiae, the lactobacillus plantarum and the lactobacillus rhamnosus is 1% -10% of the mass of the solid fermentation culture medium in the step (2); the protease is acid protease and papain; the total addition amount of the acid protease and the papain is 0.01% -2% of the mass of the solid fermentation medium in the step (2), the addition ratio of the acid protease to the papain is 1:1, and the enzyme activities are 6 ten thousand u/g;
wherein the inorganic mineral salt is CaCO 3 、FeSO 4 ·7H 2 O、CuSO 4 ·5H 2 O、MnSO 4 ·H 2 O、ZnSO 4 ·7H 2 O; said CaCO 3 The addition amount is the solid fermentation culture medium in the step (2)1% -12% of the weight of the composition; the FeSO 4 ·7H 2 The addition amount of O is 0.01-1.6% of the mass of the solid fermentation medium in the step (2); the CuSO 4 ·5H 2 The addition amount of O is 0.001% -0.064% of the mass of the solid fermentation medium in the step (2); the MnSO 4 ·H 2 The addition amount of O is 0.01% -1% of the mass of the solid fermentation medium in the step (2); the ZnSO is 4 ·7H 2 The addition amount of O is 0.01% -1.2% of the mass of the solid fermentation medium in the step (2).
2. The use according to claim 1, characterized in that in step (3); the adding amount of the secondary powder is 5% -30% of the mass of the solid fermentation medium in the step (2).
3. The use according to claim 1, wherein the water content in step (3) is 25% -65%.
4. The use according to claim 1, characterized in that it is specifically operated such that said Gao Xiaoao combined type ferment peptide is mixed with poultry feed, and said Gao Xiaoao combined type ferment peptide is used in an amount of 5% -10% by weight of poultry feed.
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| CN110074252A (en) * | 2019-05-20 | 2019-08-02 | 浙江康星生物科技有限公司 | A kind of the fermented feed processing method and application of the high efficiency, low cost based on bean dregs |
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| BR112018009235B1 (en) * | 2015-11-09 | 2022-11-22 | Dupont Nutrition Biosciences Aps | FOOD ADDITIVE COMPOSITION, METHOD OF PREPARING THE SAME, USE OF A DIRECT FOOD MICROORGANISM, KIT, FOOD COMPOSITION, AND PREMIX |
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| CN110074252A (en) * | 2019-05-20 | 2019-08-02 | 浙江康星生物科技有限公司 | A kind of the fermented feed processing method and application of the high efficiency, low cost based on bean dregs |
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