CN113502251A - Preparation method of anti-corrosion wax-control compound microbial agent for oil well exploitation - Google Patents
Preparation method of anti-corrosion wax-control compound microbial agent for oil well exploitation Download PDFInfo
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 41
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 32
- 239000003129 oil well Substances 0.000 title claims abstract description 24
- 150000001875 compounds Chemical class 0.000 title claims abstract description 20
- 238000005260 corrosion Methods 0.000 title claims abstract description 19
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 230000007797 corrosion Effects 0.000 claims abstract description 14
- 238000000855 fermentation Methods 0.000 claims description 37
- 230000004151 fermentation Effects 0.000 claims description 37
- 235000015097 nutrients Nutrition 0.000 claims description 25
- 239000000126 substance Substances 0.000 claims description 22
- 238000001816 cooling Methods 0.000 claims description 18
- 230000001954 sterilising effect Effects 0.000 claims description 14
- 241000589516 Pseudomonas Species 0.000 claims description 13
- 102000004139 alpha-Amylases Human genes 0.000 claims description 13
- 108090000637 alpha-Amylases Proteins 0.000 claims description 13
- 229940024171 alpha-amylase Drugs 0.000 claims description 13
- 238000003756 stirring Methods 0.000 claims description 12
- 244000017020 Ipomoea batatas Species 0.000 claims description 10
- 235000002678 Ipomoea batatas Nutrition 0.000 claims description 10
- 229930195733 hydrocarbon Natural products 0.000 claims description 10
- 150000002430 hydrocarbons Chemical class 0.000 claims description 10
- 229920001592 potato starch Polymers 0.000 claims description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
- 239000004215 Carbon black (E152) Substances 0.000 claims description 9
- 238000005536 corrosion prevention Methods 0.000 claims description 9
- 230000003647 oxidation Effects 0.000 claims description 9
- 238000007254 oxidation reaction Methods 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 241000588986 Alcaligenes Species 0.000 claims description 7
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 7
- 241001057811 Paracoccus <mealybug> Species 0.000 claims description 7
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 7
- 229910052698 phosphorus Inorganic materials 0.000 claims description 7
- 239000011574 phosphorus Substances 0.000 claims description 7
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 6
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- 229910002651 NO3 Inorganic materials 0.000 claims description 6
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims description 6
- 239000001888 Peptone Substances 0.000 claims description 6
- 108010080698 Peptones Proteins 0.000 claims description 6
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- 239000011777 magnesium Substances 0.000 claims description 6
- 229910052749 magnesium Inorganic materials 0.000 claims description 6
- 235000019319 peptone Nutrition 0.000 claims description 6
- 239000011591 potassium Substances 0.000 claims description 6
- 229910052700 potassium Inorganic materials 0.000 claims description 6
- 239000011734 sodium Substances 0.000 claims description 6
- 229910052708 sodium Inorganic materials 0.000 claims description 6
- 235000008733 Citrus aurantifolia Nutrition 0.000 claims description 4
- 235000011941 Tilia x europaea Nutrition 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 239000004571 lime Substances 0.000 claims description 4
- 229910003002 lithium salt Inorganic materials 0.000 claims description 4
- 159000000002 lithium salts Chemical class 0.000 claims description 4
- 238000001556 precipitation Methods 0.000 claims description 4
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- 239000002367 phosphate rock Substances 0.000 claims description 3
- OJMIONKXNSYLSR-UHFFFAOYSA-N phosphorous acid Chemical compound OP(O)O OJMIONKXNSYLSR-UHFFFAOYSA-N 0.000 claims description 3
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims description 3
- 229910052939 potassium sulfate Inorganic materials 0.000 claims description 3
- 235000011151 potassium sulphates Nutrition 0.000 claims description 3
- 239000004382 Amylase Substances 0.000 claims description 2
- 102000013142 Amylases Human genes 0.000 claims description 2
- 108010065511 Amylases Proteins 0.000 claims description 2
- 235000019418 amylase Nutrition 0.000 claims description 2
- 239000010812 mixed waste Substances 0.000 claims description 2
- 238000000605 extraction Methods 0.000 abstract description 2
- 239000003921 oil Substances 0.000 description 15
- 239000001993 wax Substances 0.000 description 15
- 239000012188 paraffin wax Substances 0.000 description 11
- 239000010779 crude oil Substances 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 6
- 230000002265 prevention Effects 0.000 description 6
- 239000002351 wastewater Substances 0.000 description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 4
- 229910052799 carbon Inorganic materials 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 description 3
- 239000013543 active substance Substances 0.000 description 3
- 229910001424 calcium ion Inorganic materials 0.000 description 3
- 239000013043 chemical agent Substances 0.000 description 3
- 230000004060 metabolic process Effects 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 239000003208 petroleum Substances 0.000 description 3
- 229910001414 potassium ion Inorganic materials 0.000 description 3
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 2
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000002421 anti-septic effect Effects 0.000 description 2
- 239000003899 bactericide agent Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 2
- 229910001425 magnesium ion Inorganic materials 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 229910001415 sodium ion Inorganic materials 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000010911 Enzyme Precursors Human genes 0.000 description 1
- 108010062466 Enzyme Precursors Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 239000013064 chemical raw material Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 238000000518 rheometry Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/52—Compositions for preventing, limiting or eliminating depositions, e.g. for cleaning
- C09K8/524—Compositions for preventing, limiting or eliminating depositions, e.g. for cleaning organic depositions, e.g. paraffins or asphaltenes
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/54—Compositions for in situ inhibition of corrosion in boreholes or wells
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/58—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
- C09K8/582—Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids characterised by the use of bacteria
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- General Life Sciences & Earth Sciences (AREA)
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- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
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- Tropical Medicine & Parasitology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
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Abstract
The invention relates to a preparation method of an anti-corrosion and anti-wax compound microbial agent for oil well exploitation. The oil well exploitation provided by the invention adopts the preparation method of the corrosion-resistant and wax-resistant compound microbial agent, thereby achieving the purposes of corrosion resistance, wax resistance and convenience in oil extraction.
Description
Technical Field
The invention belongs to the technical field of oilfield chemical preparations, and particularly relates to a preparation method of an anti-corrosion and anti-wax compound microbial agent for oil well exploitation.
Background
Petroleum is an important energy source and chemical raw material nowadays, and with the rapid development of economy and science all over the world and the increasing demand of people for petroleum, the exploration and large-scale development of petroleum resources are further pulled. In the process of oil exploitation, wax in crude oil is often separated out, grown and deposited in an oil pipe to cause wax precipitation of an oil well, and meanwhile, hydrogen sulfide is generated by sulfate reducing bacteria in the oil well and has a strong corrosion effect on metal pipes and equipment of the oil well. Therefore, effective measures must be taken to control microbial corrosion of the oil well.
The methods for paraffin removal and prevention of oil wells are various, and the methods can be classified into mechanical paraffin removal, thermal cleaning paraffin removal, chemical agent paraffin removal and microbial paraffin removal. The mechanical paraffin removal stability is not strong, and the time and the labor are wasted; the hot washing paraffin removal paraffin prevention damages the stratum, the equipment investment is large, and the production time is influenced; chemical wax control affects the quality of oil products, and the labor intensity is high; the microbial paraffin removal and prevention technology has the advantages of simple construction, low operation cost, long action period, no influence on the quality of oil and no damage to the stratum. The microorganisms generate various chemical substances such as organic acid, surfactant and the like through a metabolic process, and the effects of reducing the oil-water interfacial tension, emulsifying, reducing the viscosity of crude oil, reducing the condensation point of the crude oil, changing the components of the crude oil, improving the crude oil rheology and further avoiding the crystallization of paraffin in a tubular column in a shaft and near the shaft can be achieved.
At present, the chemical method is mainly used for controlling the microbial corrosion of an oil well, sulfate reducing bacteria are killed by adding chemical bactericide, and then the corrosion generated by hydrogen sulfide is controlled, but the chemical bactericide has the defects of high cost, easy generation of drug resistance and the like.
A large amount of waste water is generated in lithium salt industry and phosphorus chemical industry, the waste water is generally neutralized, precipitated and filtered by lime, the formed high-salinity waste water is directly discharged into a sewage treatment station for treatment, and the high-salinity waste water contains a large amount of sodium ions, calcium ions, chloride ions and the like, so that the treatment difficulty is high, and a large burden is caused on the environment.
At present, few reports are provided for microbial agents with corrosion and wax resistance, so that a preparation method which can not only prevent corrosion and wax but also reduce environmental pressure is needed.
Disclosure of Invention
The invention aims to solve the problems and provides a preparation method of the anti-corrosion and anti-wax compound microbial agent for oil well exploitation, so that the aims of corrosion resistance, wax resistance and convenience in oil extraction are fulfilled. The invention is realized by adopting the following technical scheme: a preparation method of a corrosion-resistant wax-resistant compound microbial agent for oil well exploitation comprises the following steps:
step S1, taking 55-75 parts of treated nutrient solution, 2-4 parts of nitrate, 3-7 parts of white oil and 0.5-1 part of peptone;
step S2, adding the substances in the step S1 into a vacuum fermentation tank, uniformly stirring, and heating for sterilization;
step S3, cooling after sterilization, and adding 5-10 parts of hydrocarbon oxidation strains, 5-10 parts of denitrification strains and 2-3 parts of sweet potato starch into a vacuum fermentation tank;
step S4, adding amylase into the vacuum fermentation tank, preserving heat for a period of time, and then cooling again;
step S5, fermenting to obtain the microbial agent;
the treated nutrient solution: the method comprises the steps of further adding potassium sulfate and magnesium sulfate into mixed waste water subjected to lime precipitation and filtration in lithium salt industry and phosphorite industry, concentrating and filtering to obtain a nutrient solution, and adjusting the proportion, wherein the mass concentration of main nutrient substances in the nutrient solution is 3-8% of sodium, 2-5% of potassium, 1-3% of magnesium and 1-5% of phosphorus according to the percentage.
The hydrocarbon oxidation strain comprises 80-90% of pseudomonas and 10-20% of bacillus by weight percentage, and the denitrification strain comprises 70-80% of pseudomonas, 10-15% of alcaligenes and 10-15% of paracoccus by weight percentage.
The sterilization condition of the step S2 is 121 ℃ and 30 minutes.
And when microbial strains are added in the step S3, the temperature in the vacuum fermentation tank is 85-95 ℃.
And S4, adding high-temperature alpha-amylase, wherein 0.6g of high-temperature alpha-amylase is added to each liter of fermentation liquor, and keeping the temperature for 25-30 minutes.
And after the temperature is reduced in the step S4, the temperature in the vacuum fermentation tank is 55-65 ℃.
And S5, fermenting for 2-3 days, and cooling to 15-35 ℃ while stirring after fermentation is completed to obtain the microbial agent.
The invention has the beneficial effects that:
1. according to the invention, after lime is added to precipitate and filter the waste water in lithium salt industry and phosphorite industry, potassium sulfate and magnesium sulfate are added to further remove calcium ions in the solution, the liquid is concentrated and filtered to obtain nutrient solution, potassium ions and magnesium ions are added while removing the calcium ions, the sodium ions and the potassium ions can provide external environment for microbial growth, and the potassium ions and the magnesium ions are activators of a plurality of enzymes and can promote metabolism of carbohydrates, synthesis of nucleic acid and conversion of phosphate. The nutrient solution obtained by treating the wastewater can fully utilize the nutrient substances in the water and reduce the environmental pressure at the same time.
2. The invention selects the strains of pseudomonas, bacillus, alcaligenes and paracoccus, takes the growth, reproduction and metabolism conditions of hydrocarbon oxidation strains and denitrifying strains into consideration, the two strains of facultative anaerobes belonging to the same genus can grow, reproduce and metabolize in the same environment, good wax-proof and antiseptic performances are respectively kept, the growth of sulfate reducing bacteria is balanced, the metabolite of the hydrocarbon oxidation strains can also provide a carbon source for the denitrifying strains, the growth and reproduction of the denitrifying strains are promoted, the two strains are mutually matched and cooperated, the fermentation temperature in the strict preparation process is ensured, the balance of the ratio of the two strains is ensured, and thus the microbial agent keeps the best wax-proof and antiseptic effects.
3. According to the invention, the sweet potato starch and the high-temperature alpha-amylase are added together while the microbial strains are added, and the adding temperature is 85-95 ℃, so that on one hand, strains capable of adapting to the temperature can be selected, on the other hand, the sweet potato starch can be gelatinized at the temperature, and can be rapidly decomposed into small molecular substances under the enzymolysis effect of the high-temperature alpha-amylase, so that a carbon source and a nitrogen source are provided for the growth of microorganisms, and the carbon source and the nitrogen source provided after the gelatinization and enzymolysis of the sweet potato starch can also influence the generation content of surface active substances in the metabolic activity process of the microorganisms.
Detailed Description
The technical solutions in the examples will be clearly and completely described below. It is to be understood that the described embodiments are merely a few embodiments of the invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the present invention without any inventive step, are within the scope of the present invention.
Example 1
Step S1, taking 55kg of processed nutrient solution, wherein the mass concentration of main nutrient substances in the nutrient solution is 3 percent of sodium, 2 percent of potassium, 1 percent of magnesium, 1 percent of phosphorus, 2kg of nitrate, 3kg of white oil and 0.5kg of peptone;
step S2, adding the substances in the step S1 into a vacuum fermentation tank, uniformly stirring, heating to 121 ℃, and sterilizing for 30 minutes;
step S3, cooling to 85 ℃ after sterilization, oxidizing 5kg of strains according to hydrocarbons, wherein 4kg of pseudomonas, 1kg of bacillus and 5kg of denitrifying strains are contained, wherein 3.5kg of pseudomonas, 0.75kg of alcaligenes, 0.75kg of paracoccus and 2kg of sweet potato starch are added into a vacuum fermentation tank;
step S4, adding high-temperature alpha-amylase to the vacuum fermentation tank, adding 0.6g of high-temperature alpha-amylase to each liter of fermentation liquor, preserving heat for 25 minutes, and then cooling to 55 ℃ again;
and step S5, fermenting for 2 days, and cooling to 15 ℃ while stirring after fermentation is finished to obtain the microbial agent.
Example 2
Step S1, taking 75kg of processed nutrient solution, wherein the mass concentration of main nutrient substances in the nutrient solution is 8 percent of sodium, 5 percent of potassium, 3 percent of magnesium, 5 percent of phosphorus, 4kg of nitrate, 7kg of white oil and 1kg of peptone;
step S2, adding the substances in the step S1 into a vacuum fermentation tank, uniformly stirring, heating to 121 ℃, and sterilizing for 30 minutes;
step S3, cooling to 95 ℃ after sterilization, and adding 10kg of hydrocarbon oxidation strains, 9kg of pseudomonas, 1kg of bacillus, 10kg of denitrifying strains, 8kg of pseudomonas, 1kg of alcaligenes, 1kg of paracoccus and 3kg of sweet potato starch into a vacuum fermentation tank;
step S4, adding high-temperature alpha-amylase to the vacuum fermentation tank, adding 0.6g of high-temperature alpha-amylase to each liter of fermentation liquor, preserving heat for 30 minutes, and then cooling to 65 ℃ again;
and step S5, fermenting for 3 days, and cooling to 35 ℃ while stirring after fermentation is finished to obtain the microbial agent.
Example 3
Step S1, taking 65kg of processed nutrient solution, wherein the mass concentration of main nutrient substances in the nutrient solution is, by percentage, 5% of sodium, 3% of potassium, 2% of magnesium, 3% of phosphorus, 3kg of nitrate, 5kg of white oil and 0.75kg of peptone;
step S2, adding the substances in the step S1 into a vacuum fermentation tank, uniformly stirring, heating to 121 ℃, and sterilizing for 30 minutes;
step S3, cooling to 90 ℃ after sterilization, adding 7.5kg of hydrocarbon oxidation strains, 6.4kg of pseudomonas, 1.1kg of bacillus, 7.5kg of denitrifying strains, 5.6kg of pseudomonas, 0.95kg of alcaligenes, 0.95kg of paracoccus and 2.5kg of sweet potato starch into a vacuum fermentation tank;
step S4, adding high-temperature alpha-amylase to the vacuum fermentation tank, adding 0.6g of high-temperature alpha-amylase to each liter of fermentation liquor, preserving heat for 25-30 minutes, and then cooling to 55-65 ℃ again;
and step S5, fermenting for 2-3 days, and cooling to 15-35 ℃ while stirring after fermentation is completed to obtain the microbial agent.
Example 4
Step S1, taking 65 parts of the processed nutrient solution, wherein the mass concentration of main nutrient substances in the nutrient solution is 5 percent of sodium, 3 percent of potassium, 2 percent of magnesium, 3 percent of phosphorus, 3kg of nitrate, 5kg of white oil and 0.75kg of peptone;
step S2, adding the substances in the step S1 into a vacuum fermentation tank, uniformly stirring, heating to 121 ℃, and sterilizing for 30 minutes;
step S3, cooling to 90 ℃ after sterilization, adding 7.5kg of hydrocarbon oxidation strains, 6.4kg of pseudomonas, 1.1kg of bacillus, 7.5kg of denitrifying strains, 5.6kg of pseudomonas, 0.95kg of alcaligenes, 0.95kg of paracoccus and 2.5kg of sucrose into a vacuum fermentation tank;
step S4, preserving heat for 25-30 minutes, and then cooling to 55-65 ℃ again;
and step S5, fermenting for 2-3 days, and cooling to 15-35 ℃ while stirring after fermentation is completed to obtain the microbial agent.
The microbial inoculum prepared by the preparation method of the embodiment 1-4 is obtained, equal amounts of four fermentation liquids are taken to measure the bacterial concentration of the fermentation liquids, oil and water produced by an oil well in a certain area of the south of the Wen oil plant are taken, the equal amounts of the four fermentation liquids are respectively added into the taken oil and water produced, after the four fermentation liquids are cultured for a period of time, the viscosities of the oil and water produced are respectively measured, and the results are shown in Table 1.
TABLE 1 measurement results of examples
As can be seen from the above table, in comparative examples 1 to 3, the growth condition of bacteria is normal, in comparative examples 3 and 4, sweet potato starch and high-temperature alpha-amylase are added in example 3, sucrose is used instead of example 4, and the concentration and viscosity reduction rate of bacteria in example 3 are significantly higher than those in example 4, which indicates that the carbon source and the nitrogen source provided after gelatinization and enzymolysis of sweet potato starch can influence the generation content of surface active substances in the process of microbial metabolic activity, and the content of surface active substances is increased, so that the viscosity reduction rate is improved.
The enlarged production of the embodiment 3 is carried out to obtain 1800kg of zymogen liquid, an oil well is arranged in a certain operation area of a first oil production plant of the Wen south oilfield, the temperature of the oil well is 87 ℃, the pH value of the oil well is 6.9, the average water content of the produced liquid of the well is more than 65%, the sulfate reducing bacteria content is 95/mL, the average corrosion rate is 0.12mm/a, the average wax content of crude oil is more than 10%, a shaft is very serious in wax deposition, the shaft is corroded after long-time use, 15 kg/week of chemical paraffin cleaner is added originally, the shaft needs to be heated and washed once per month, the loss caused by the reduction of crude oil collection due to the use amount of chemical agents and each heated and washed per month is up to 4.5 ten thousand yuan, and therefore, the cost of the chemical agents for wax prevention and corrosion prevention in each year of the well is about 54 ten thousand yuan.
After the wax-proof and corrosion-resistant microbial agent is used for the well, 150 kilograms of microbial agent is added every month, the well can be kept from influencing normal production due to wax precipitation after being hot-washed once every 4 months, and the average corrosion rate is below 0.076 mm/a. Therefore, the cost and the loss of the well for wax prevention and corrosion prevention are only about 15 ten thousand yuan each year, the economic benefit is high, and the wax prevention and corrosion prevention effects are better.
Claims (7)
1. A preparation method of a corrosion-resistant wax-resistant compound microbial agent for oil well exploitation is characterized by comprising the following steps:
step S1, taking 55-75 parts of treated nutrient solution, 2-4 parts of nitrate, 3-7 parts of white oil and 0.5-1 part of peptone;
step S2, adding the substances in the step S1 into a vacuum fermentation tank, uniformly stirring, and heating for sterilization;
step S3, cooling after sterilization, and adding 5-10 parts of hydrocarbon oxidation strains, 5-10 parts of denitrification strains and 2-3 parts of sweet potato starch into a vacuum fermentation tank;
step S4, adding amylase into the vacuum fermentation tank, preserving heat for a period of time, and then cooling again;
step S5, fermenting to obtain the microbial agent;
the treated nutrient solution: the method comprises the steps of further adding potassium sulfate and magnesium sulfate into mixed waste water subjected to lime precipitation and filtration in lithium salt industry and phosphorite industry, concentrating and filtering to obtain a nutrient solution, and adjusting the proportion, wherein the mass concentration of main nutrient substances in the nutrient solution is 3-8% of sodium, 2-5% of potassium, 1-3% of magnesium and 1-5% of phosphorus according to the percentage.
2. The preparation method of the compound microbial agent for corrosion prevention and wax control for oil well exploitation according to claim 1, wherein the compound microbial agent comprises the following steps: the hydrocarbon oxidation strain comprises 80-90% of pseudomonas and 10-20% of bacillus by weight percentage, and the denitrification strain comprises 70-80% of pseudomonas, 10-15% of alcaligenes and 10-15% of paracoccus by weight percentage.
3. The preparation method of the compound microbial agent for corrosion prevention and wax control for oil well exploitation according to claim 1, wherein the compound microbial agent comprises the following steps: the sterilization condition of the step S2 is 121 ℃ and 30 minutes.
4. The preparation method of the compound microbial agent for corrosion prevention and wax control for oil well exploitation according to claim 1, wherein the compound microbial agent comprises the following steps: and when microbial strains are added in the step S3, the temperature in the vacuum fermentation tank is 85-95 ℃.
5. The preparation method of the compound microbial agent for corrosion prevention and wax control for oil well exploitation according to claim 1, wherein the compound microbial agent comprises the following steps: and S4, adding high-temperature alpha-amylase, wherein 0.6g of high-temperature alpha-amylase is added to each liter of fermentation liquor, and keeping the temperature for 25-30 minutes.
6. The preparation method of the compound microbial agent for corrosion prevention and wax control for oil well exploitation according to claim 1, wherein the compound microbial agent comprises the following steps: and after the temperature is reduced in the step S4, the temperature in the vacuum fermentation tank is 55-65 ℃.
7. The preparation method of the compound microbial agent for corrosion prevention and wax control for oil well exploitation according to claim 1, wherein the compound microbial agent comprises the following steps: and S5, fermenting for 2-3 days, and cooling to 15-35 ℃ while stirring after fermentation is completed to obtain the microbial agent.
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