CN113501575A - In-situ biological membrane carrier reactor for rapid BOD detection - Google Patents
In-situ biological membrane carrier reactor for rapid BOD detection Download PDFInfo
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- 238000001514 detection method Methods 0.000 title claims abstract description 103
- 239000012528 membrane Substances 0.000 title claims abstract description 76
- 238000011065 in-situ storage Methods 0.000 title claims abstract description 57
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 63
- 239000001301 oxygen Substances 0.000 claims abstract description 63
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 62
- 244000005700 microbiome Species 0.000 claims abstract description 52
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 48
- 238000009434 installation Methods 0.000 claims abstract description 3
- 238000003860 storage Methods 0.000 claims description 34
- 238000004140 cleaning Methods 0.000 claims description 29
- 239000002699 waste material Substances 0.000 claims description 23
- 239000000758 substrate Substances 0.000 claims description 12
- 235000015097 nutrients Nutrition 0.000 claims description 10
- 238000005276 aerator Methods 0.000 claims description 6
- 238000004891 communication Methods 0.000 claims description 5
- 230000035699 permeability Effects 0.000 claims description 3
- 239000007788 liquid Substances 0.000 description 14
- 238000000034 method Methods 0.000 description 13
- 230000000813 microbial effect Effects 0.000 description 11
- 239000000463 material Substances 0.000 description 7
- 230000008569 process Effects 0.000 description 7
- 238000005406 washing Methods 0.000 description 7
- 238000012544 monitoring process Methods 0.000 description 6
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000011010 flushing procedure Methods 0.000 description 3
- 230000036284 oxygen consumption Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 238000011001 backwashing Methods 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000005574 cross-species transmission Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
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- 239000003344 environmental pollutant Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000012808 pre-inoculation Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/005—Combined electrochemical biological processes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D65/00—Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes
- B01D65/02—Membrane cleaning or sterilisation ; Membrane regeneration
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/12—Activated sludge processes
- C02F3/1236—Particular type of activated sludge installations
- C02F3/1268—Membrane bioreactor systems
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/1806—Biological oxygen demand [BOD] or chemical oxygen demand [COD]
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F2303/00—Specific treatment goals
- C02F2303/14—Maintenance of water treatment installations
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
The invention provides an in-situ biofilm carrier reactor for rapid BOD detection, which comprises a detection channel, a first dissolved oxygen electrode, a second dissolved oxygen electrode, an in-situ biofilm component and a driving device, the detection channel is of a pipeline structure with openings at two ends, the in-situ biological membrane component is arranged on the side wall of the detection channel along the length direction of the detection channel and is used for acquiring microorganisms in a water sample flowing through the inner side of the detection channel and carrying out in-situ growth culture, therefore, dissolved oxygen in a water sample flowing through the detection channel is consumed, the first dissolved oxygen electrode and the second dissolved oxygen electrode are respectively installed on the pipe walls of the detection channel, which are located on two sides of the in-situ biological membrane assembly, and are used for detecting the dissolved oxygen in the water sample flowing through the installation positions of the first dissolved oxygen electrode and the second dissolved oxygen electrode, and the driving device is used for driving the water sample in the detection channel to flow. The invention can be suitable for the rapid BOD detection of different water body environments and has good application prospect.
Description
Technical Field
The invention relates to the technical field of water body detection equipment, in particular to an in-situ biofilm carrier reactor for rapid BOD detection.
Background
The online monitoring technology of the parameters such as the conventional five parameters of water quality, ammonia nitrogen, total phosphorus, total nitrogen, chemical oxygen demand and the like is relatively mature, but for the Biochemical Oxygen Demand (BOD), the international implementation means are more at present, but no mature online BOD monitoring equipment exists.
The existing BOD monitoring method mainly comprises a five-day biochemical culture method and a microbial electrode method, and the basic principle of the five-day biochemical culture method is as follows: filling a water sample into a completely closed dissolved oxygen bottle, culturing for five days in a dark place at 20 +/-1 ℃, measuring the mass concentration of dissolved oxygen in the water sample before and after culturing in decibels, and calculating the concentration difference to obtain the dissolved oxygen consumed by each liter of sample so as to obtain BOD5, wherein the method needs manual operation, the culture environment needs to be maintained stable, consumes time and labor and is not beneficial to rapid monitoring; the basic principle of the microbial electrode method is as follows: the sensor is formed by combining the dissolved oxygen electrode and the microbial film, when a sample containing saturated dissolved oxygen enters the flow cell and contacts with the microbial sensor, dissolved organic matters which are soluble and biodegradable in the sample are acted by strains in the microbial film to consume a certain amount of dissolved oxygen, so that the quality of oxygen diffused to the surface of the oxygen electrode is reduced. When the diffusion speed of the biodegradable organic matter in the sample to the bacterial membrane reaches a constant, the quality of oxygen diffused to the surface of the oxygen electrode also reaches a constant, so that a constant current is generated, the biochemical oxygen demand can be converted according to the relation between the current difference and the oxygen consumption, the regulation in the relevant standard is that the microbial membrane is formed by sealing specific microorganisms into the membrane under the state of keeping the physiological functions of the microorganisms, the specific bacterial membrane is possibly different from the types of the microorganisms or domesticated microorganisms in the water body to be detected and contradicts the idea of BOD detection, meanwhile, the bacterial membrane in the prior art is invaded by strains in a water sample in the using process, the preselection of the specific strains is lost, the strains are difficult to survive for a long time in the actual using process, the microbial membrane needs to be frequently replaced in the actual application, and the objective factors cause inaccurate rapid detection result, the BOD detection result, The detection method is complicated.
Disclosure of Invention
In view of the above, the invention provides the in-situ biofilm carrier reactor for rapid BOD detection, which has a more reasonable structural design, can be adapted to different detection water bodies, and is more convenient to operate.
The technical scheme of the invention is realized as follows: the invention provides an in-situ biological membrane carrier reactor for rapid BOD detection, which comprises a detection channel, a first dissolved oxygen electrode, a second dissolved oxygen electrode, an in-situ biological membrane component and a driving device, the detection channel is of a pipeline structure with openings at two ends, the in-situ biological membrane component is arranged on the side wall of the detection channel along the length direction of the detection channel and is used for acquiring microorganisms in a water sample flowing through the inner side of the detection channel and carrying out in-situ growth culture, therefore, dissolved oxygen in a water sample flowing through the detection channel is consumed, the first dissolved oxygen electrode and the second dissolved oxygen electrode are respectively installed on the pipe walls of the detection channel, which are located on two sides of the in-situ biological membrane assembly, and are used for detecting the dissolved oxygen in the water sample flowing through the installation positions of the first dissolved oxygen electrode and the second dissolved oxygen electrode, and the driving device is used for driving the water sample in the detection channel to flow.
On the basis of the technical scheme, preferably, the in-situ biological membrane assembly comprises a first cavity, a first filter membrane, a first feeding assembly and a first receiving assembly, the first cavity is communicated with the detection channel through the first filter membrane, one end of the first cavity is communicated with the first feeding assembly, the other end of the first cavity is communicated with the first receiving assembly, the first feeding assembly selectively provides nutrient media into the first cavity, and the first receiving assembly is used for containing the nutrient media discharged from the first cavity.
On the basis of the technical scheme, preferably, the first feeding assembly comprises a substrate storage tank, a first feeding pump and a first valve, the substrate storage tank is communicated with one end of the first cavity through the feeding pump and the first valve in sequence, the first receiving assembly comprises a first waste tank and a second valve, and the other end of the first cavity is communicated with the first waste tank through the second valve.
On the basis of the technical scheme, the device is preferred to be further provided with a second cavity, a second filter membrane, a second feeding assembly and a second receiving assembly, the second cavity is communicated with the detection channel through the second filter membrane, the first cavity is communicated with the second cavity through the first filter membrane, one end of the second cavity is communicated with the second feeding assembly, the other end of the second cavity is communicated with the second receiving assembly, the second feeding assembly selectively provides cleaning liquid into the second cavity, and the second receiving assembly is used for containing the cleaning liquid and microorganisms discharged from the second cavity.
Still further preferably, the second feeding assembly comprises a cleaning liquid storage tank, a second feeding pump and a third valve, the cleaning liquid storage tank is communicated with one end of the second cavity through the second feeding pump and the third valve in sequence, the second receiving assembly comprises a second waste material tank and a fourth valve, and the other end of the second cavity is communicated with the second waste material tank through the fourth valve.
On the basis of the above technical scheme, preferably, the gel detection device further comprises a third cavity, a third filter membrane, a third feeding assembly and a third receiving assembly, wherein the third cavity is communicated with the detection channel through the third filter membrane, the second cavity is communicated with the third cavity through the second filter membrane, the first cavity is communicated with the second cavity through the first filter membrane, one end of the third cavity is communicated with the third feeding assembly, the other end of the third cavity is communicated with the third receiving assembly, the third feeding assembly selectively provides gel with selective permeability into the third cavity, and the third receiving assembly is used for containing the gel discharged from the third cavity.
On the basis of the technical scheme, preferably, the third feeding assembly comprises a gel storage tank, a third feeding pump and a fifth valve, the gel storage tank is communicated with one end of the third cavity sequentially through the third feeding pump and the fifth valve, the third receiving assembly comprises a third waste material tank and a sixth valve, and the other end of the third cavity is communicated with the third waste material tank through the sixth valve.
On the basis of above technical scheme, preferably, the third feed subassembly still includes gel valve, washing liquid storage tank and flush valve, and the gel storage tank communicates with each other through gel valve and third feed pump, and the pipeline between washing liquid storage tank and gel valve and the third feed pump communicates with each other through flush valve.
On the basis of the above technical solution, preferably, the driving device is a water pump.
On the basis of the technical scheme, the automatic aerator is preferred, and the automatic aerator is installed at one end, away from the driving device, of the detection channel.
Compared with the prior art, the in-situ biofilm carrier reactor for rapid BOD detection has the following beneficial effects:
(1) the invention provides an in-situ biofilm carrier reactor for rapid BOD detection, the microbial membrane required by rapid BOD detection is prepared by acquiring the in-situ growth of the microorganisms from the water body to be detected, the problem that the microbial membrane is easily polluted in the prior BOD rapid detection is solved, meanwhile, the microorganism culture is carried out by adopting an in-situ growth mode, which is beneficial to improving the activity of the microorganism, thereby ensuring the stability of microorganisms in a detection period, improving the accuracy of a detection result, leading the use of the reactor of the invention to be more convenient by adopting in-situ growth, leading the conventional detection reactor to need to regularly replace a biological membrane, having complicated and inconvenient steps, saving the step by the proposal of the invention, the automatic growth and BOD detection of microorganisms are realized by controlling the internal device of the in-situ biofilm carrier reactor;
(2) whole device simple structure through the structural optimization to normal position biomembrane subassembly, makes the biomembrane can reuse, is adapted to different water environment and microorganism growth demand, compares conventional biomembrane simultaneously, and the biomembrane subassembly of this application can hold more microorganism, and the cardinal number of oxygen consumption is bigger, and robustness is higher, detects the difficult data deviation that appears of structure, and data accuracy is higher.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without any creative effort.
FIG. 1 is a front view of a rapid BOD detection in situ biofilm carrier reactor of the present invention;
FIG. 2 is a front view of another embodiment of the rapid BOD detection in situ biofilm carrier reactor of the present invention;
FIG. 3 is a front view of an in situ biofilm assembly of the rapid BOD detection in situ biofilm carrier reactor of the present invention;
FIG. 4 is a front view of the partial structure of the in situ biofilm component of the rapid BOD detection in situ biofilm carrier reactor of the present invention.
In the figure: 1-detection channel, 2-first dissolved oxygen electrode, 3-second dissolved oxygen electrode, 4-in-situ biological membrane component, 5-driving device, 6-oxygen increasing machine, 401-first cavity, 402-first filter membrane, 403-first feeding component, 404-first material receiving component, 405-second cavity, 406-second filter membrane, 407-second feeding component, 408-second material receiving component, 409-third cavity, 410-third filter membrane, 411-third feeding component, 412-third material receiving component, 4031-matrix storage tank, 4032-first feeding pump, 4033-first valve, 4041-first waste tank, 4042-second valve, 4071-cleaning solution storage tank, 4072-second feeding pump, 4073-third valve, 4081-second canister, 4082-fourth valve, 4111-gel tank, 4112-third feed pump, 4113-fifth valve, 4121-third waste tank, 4122-sixth valve, 4114-gel valve, 4115-flushing liquid tank, 4116-flushing valve.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
As shown in fig. 1, with reference to fig. 2-4, the rapid BOD detection in-situ biofilm carrier reactor of the present invention includes a detection channel 1, a first dissolved oxygen electrode 2, a second dissolved oxygen electrode 3, an in-situ biofilm assembly 4, and a driving device 5, in a specific embodiment, the detection channel 1 is a tubular structure, the first dissolved oxygen electrode 2 and the second dissolved oxygen electrode 3 are respectively installed on the side surfaces of both ends of the detection channel 1 along the length direction thereof, the detection ends of both dissolved oxygen electrodes are located on the inner side of the detection channel 1, the in-situ biofilm assembly 4 is installed on the side wall of the detection channel 1 at a position between the two dissolved oxygen electrodes, the in-situ biofilm assembly 4 can directly contact with a water sample in the detection channel 1, and the driving device 5 is installed on one end of the detection channel 1.
In the above embodiment, the detection channel 1 and the in-situ bio-membrane module 4 are immersed in the water to be detected during the working process, the driving device 5 drives the detection water sample to flow in the detection channel 1 in a single direction, the microorganisms in the detection water sample enter the in-situ bio-membrane module 4 and grow in situ to form a bio-membrane, when the water sample flows through the in-situ bio-membrane module 4, the dissolved oxygen therein is consumed by the microorganisms in the in-situ bio-membrane module 4, so that the concentration is reduced, the dissolved oxygen level is detected in real time by the first dissolved oxygen electrode 2 and the second dissolved oxygen electrode 3 which are positioned at the front end and the rear end of the in-situ bio-membrane module 4, the consumption rate of the dissolved oxygen is obtained according to the variation trend, the final BOD detection result can be obtained through corresponding calculation, and rapid and automatic BOD detection can be realized through the above method, and the microorganisms used for detection are from the water body to be detected, so that the problems of microbial pollution and need of pre-inoculation do not exist, the detection result is accurate, the above embodiment can be theoretically applied to various water body environments, and the adaptability is better.
In a specific embodiment, in order to realize the functions of the in-situ bio-membrane module 4, the in-situ bio-membrane module 4 specifically includes a first cavity 401, a first filter membrane 402, a first supply module 403 and a first receiving module 404, the first cavity 401 is used for containing nutrients required for the growth of microorganisms, the first cavity 401 is separated from the interior of the detection channel 1 by the first filter membrane 402, the first supply module 403 and the first receiving module 404 are respectively communicated with two ends of the first cavity 401, the first filter membrane 402 can isolate mechanical impurities and microorganisms in water, but can transport nutrients required for the growth of microorganisms into the first cavity 401 through moisture and dissolved oxygen, the microorganisms in the water body are attached to the surface 403 of the first filter membrane 402 by means of taxis, a microbial membrane is formed after growth, and when the nutrients need to be supplemented and replaced, the consumed nutrients can be discharged into the first receiving module 404 for storage, and the nutrient supply can be continued through the first feeding module 403.
In the above embodiment, the microorganisms are attached to the first filter membrane 402 by providing the substances required for the growth of the microorganisms, and the microorganism membrane is finally formed for the rapid BOD detection, and the pore size of the first filter membrane is preferably 2-200 nm.
In a particular embodiment, the first supply most base 403 further comprises a substrate reservoir 4031, a first supply pump 4032 and a first valve 4033, the substrate reservoir 4031 in turn communicating with one end of the first chamber 401 via the supply pump 4032 and the first valve 4033.
In the above embodiment, the substrate storage 4031 stores the pre-configured culture substrate, and the culture substrate is delivered into the first chamber 401 by the first supply pump 4032, and the first valve 4033 is used to control the opening and closing of the delivery line, so as to prevent the liquid in the first chamber 401 from flowing back and contaminating the configured material in the substrate storage 4031 after the delivery is completed.
In a specific embodiment, as a preferred embodiment, a cleaning pipeline is further connected to the delivery pipeline between the substrate storage tank 4031 and the first feed pump 4032, and at the same time, switch valves are respectively disposed on the outlet of the substrate storage tank 4031 and the cleaning pipeline, so that the first cavity 401 can be flushed by disposing the cleaning pipeline, and the first filter membrane 402 can be backwashed, so that the first filter membrane 402 is kept clean when the reactor is not in use, thereby facilitating storage, and avoiding contamination of residual microorganisms to next use.
In the specific embodiment, the device further comprises a second cavity 405, a second filter 406, a second feeding assembly 407 and a second receiving assembly 408, wherein the second cavity 405 is communicated with the detection channel 1 through the second filter 406, the first cavity 401 is communicated with the second cavity 405 through the first filter 402, one end of the second cavity 405 is communicated with the second feeding assembly 407, the other end of the second cavity 405 is communicated with the second receiving assembly 408, the second feeding assembly 407 selectively supplies the cleaning solution into the second cavity 405, and the second receiving assembly 408 is used for receiving the cleaning solution and the microorganisms discharged from the second cavity 405.
In the above embodiment, because the first filter membrane 402 directly contacts with the liquid in the detection channel 1, the microorganisms attached to the surface of the first filter membrane 402 are not stable enough, and may fall off with the water flow washing in the detection process, which affects the accuracy of the detection result, in view of this, the above embodiment adopts a method of increasing the second cavity 405, the second filter membrane 405 is disposed on the side of the first filter membrane 402 close to the monitoring channel 1, the microorganisms in the detected water sample can directly enter the second cavity 405 through the second filter membrane 405, and attach to the surface of the first filter membrane 402 in the second cavity 405, the second cavity 405 is used as a fixed container for the growth of the microorganisms, which can effectively avoid the rapid change of the quantity of the microorganisms along with the water flow washing in the detection process, and the quantity of the microorganisms can not change greatly after the microorganisms grow to a relatively stable scale by the second cavity 405 with a fixed volume, be favorable to improving the stability of dissolved oxygen consumption measuring result, set up simultaneously that second feed assembly 407 and second receive material subassembly 408 can wash the microorganism that is located second cavity 405 to for the next microorganism growth provides the environment, be convenient for used repeatedly, can adapt to different water environment.
In a specific embodiment, the second feeding assembly 407 includes a cleaning solution storage tank 4071, a second feeding pump 4072 and a third valve 4073, the cleaning solution storage tank 4071 is communicated with one end of the second cavity 405 through the second feeding pump 4072 and the third valve 4073 in sequence, the second receiving assembly 408 includes a second waste material tank 4081 and a fourth waste material tank 4082, and the other end of the second cavity 405 is communicated with the second waste material tank 4081 through the fourth valve 4082.
In the above embodiment, the cleaning solution storage tank 4071 is used for storing the cleaning solution, and the cleaning solution is conveyed into the second cavity 405 through the second supply pump 4072 to clean the second cavity 405, the third valve 4073 can selectively cut off the communication pipeline between the second cavity 405 and the cleaning solution storage tank 4071, so as to control the circulation of the cleaning solution, and meanwhile, the microorganisms in the second cavity 405 are prevented from flowing backwards into the cleaning solution storage tank 4071, and the second waste tank 4081 is used for collecting the waste liquid after cleaning is completed, and the waste liquid is stored uniformly and is convenient to process.
In a specific embodiment, a cleaning pipeline is further connected to a pipeline between the cleaning solution storage tank 4071 and the third valve 4073, a switch valve is disposed on the cleaning pipeline, a switch valve is also disposed at an outlet of the cleaning solution storage tank 4071, and the second cavity 405 can be flushed and backwashed through the cleaning pipeline, during long-term use, some pollutants and microorganisms may be attached to the inside of the second filter membrane 406, so that the second filter membrane 406 should be cleaned as much as possible so as not to affect next BOD monitoring, therefore, after the second cavity 405 is cleaned, the second filter membrane 406 should be flushed and backwashed to ensure cleanness, and during flushing and backwashing, the corresponding valves are controlled to be opened and closed.
In the specific embodiment, the kit further comprises a third cavity 409, a third filter 410, a third feeding assembly 411 and a third receiving assembly 412, wherein the third cavity 409 is communicated with the detection channel 1 through the third filter 410, the second cavity 405 is communicated with the third cavity 409 through the second filter 406, the first cavity 401 is communicated with the second cavity 405 through the first filter 402, one end of the third cavity 409 is communicated with the third feeding assembly 411, the other end of the third cavity 409 is communicated with the third receiving assembly 412, the third feeding assembly 411 selectively provides gel with selective permeability into the third cavity 409, and the third receiving assembly 412 is used for receiving the gel discharged from the third cavity 409.
In the above embodiment, in the actual use process, after microorganisms enter the second cavity 405, the nutrients provided by the first cavity 401 can promote the rapid growth of the microorganisms, and since the second filter membrane 406 can permeate the microorganisms, the grown microorganisms may further enter the detection channel 1 through the second filter membrane 406, which is not favorable for maintaining the stability of the microorganism growth environment and is easy to pollute the local water area, therefore, in order to further improve the stability of the microorganism growth and reduce the pollution to the water, the present application further arranges the third cavity 409 between the second cavity 405 and the detection channel 1, the second cavity 405 is communicated with the third cavity 409 through the second filter membrane 406, the third cavity 409 is communicated with the detection channel 1 through the third filter membrane 410, and by injecting the gel 409 with a specific component into the third cavity, can effectively isolate the passing through of microorganism, can make dissolved oxygen in the water body etc. material pass through simultaneously, and receive material subassembly 411 and third 412, can realize changing the gel, can selectively guarantee simultaneously that third cavity 409 packs or not pack the gel to reach and let the microorganism in the detection passageway 1 selectively get into in the second cavity 405 or selectively prevent the microorganism in the second cavity 405 to spill over.
In one embodiment, the third feed assembly 411 includes a gel reservoir 4111, a third feed pump 4112, and a fifth valve 4113, wherein the gel reservoir 4111 is in communication with one end of the third cavity 409 via the third feed pump 4112 and the fifth valve 4113 at a time, and the third receiving assembly 412 includes a third waste tank 4121 and a sixth valve 4122, wherein the other end of the third cavity 409 is in communication with the third waste tank 4121 via the sixth valve 4122.
In the above embodiment, the gel storage tank 4111 is used for storing pre-prepared gel, the third feed pump 4112 is used for providing a conveying power to convey the gel in the gel storage tank 4111 to the third cavity 409, and the third waste tank 4121 is used for storing used gel, so as to achieve the purpose of centralized storage and disposal.
In an exemplary embodiment, the third feed assembly 411 further includes a gel valve 4114, a rinse reservoir 4115, and a rinse valve 4116, wherein the gel reservoir 4111 is interconnected to the third feed pump 4112 via the gel valve 4114, and the rinse reservoir 4115 is interconnected to the conduits between the gel valve 4114 and the third feed pump 4112 via the rinse valve 4116.
In the above embodiment, when the use environment needs to be changed, the microorganisms in the water body need to enter the second cavity 405 again, and therefore the gel in the third cavity 409 needs to be cleaned, the washing liquid storage tank is arranged to selectively wash and replace the gel filled in the third cavity 409 with the washing liquid, and the purpose of cleaning is achieved at the same time, the washing liquid in the third cavity 409 can allow new microorganisms to pass through after replacement, and simultaneously the surface of the third filter membrane 410 can also be washed and backwashed.
In the specific implementation mode, drive arrangement 5 is the water pump, and drive arrangement 5 is used for providing circulation power for the water sample in the measuring channel 1 to order about the water sample to flow along same direction, thereby can let first dissolved oxygen electrode 2 and second dissolved oxygen electrode 3 detect the dissolved oxygen in the water sample of different periods of time same position department respectively, as preferred implementation mode, drive arrangement 5 installs the position at measuring channel 1 delivery port, and the turbulent flow that drive arrangement produced to the measuring water sample is difficult to transmit the position of dissolved oxygen electrode like this, thereby reduces the influence to the testing result.
In the specific embodiment, an aerator 6 is installed at one end of the detection channel 1 far away from the driving device 5.
Because the amount of microorganisms in the biomembrane is relatively large in the reactor, the consumption of dissolved oxygen is high, if the amount of the dissolved oxygen in the water is lower than the required amount of the microorganisms, the inaccuracy of the detection result can be directly caused, so that the accuracy of the detection result can be effectively improved by adopting the aerator 6 to carry out aeration treatment on the water sample entering the detection channel 1, and when the base number of the dissolved oxygen is increased, the influence of the change error of the dissolved oxygen on the result is smaller, and the detection result is more accurate.
In a specific embodiment, the pore size of the second filter 406 and the third filter 410 is 2-200 μm, which allows most of the microorganisms to pass through, and at the same time, effectively prevents contaminants and mechanical impurities from entering and damaging the in-situ biofilm assembly 4.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. A rapid BOD detection in-situ biological membrane carrier reactor is characterized by comprising a detection channel (1), a first dissolved oxygen electrode (2), a second dissolved oxygen electrode (3), an in-situ biological membrane component (4) and a driving device (5), wherein the detection channel (1) is of a pipeline structure with two open ends, the in-situ biological membrane component (4) is arranged on the side wall of the detection channel (1) along the length direction of the detection channel (1), the in-situ biological membrane component (4) is used for obtaining microorganisms in a water sample flowing through the inner side of the detection channel (1) and carrying out in-situ growth culture, so that dissolved oxygen in the water sample flowing through the detection channel (1) is consumed, the first dissolved oxygen electrode (2) and the second dissolved oxygen electrode (3) are respectively arranged on the pipe walls of the detection channel (1) positioned at the two sides of the in-situ biological membrane component (4), the first dissolved oxygen electrode (2) and the second dissolved oxygen electrode (3) are used for detecting dissolved oxygen in a water sample flowing through the installation position of the first dissolved oxygen electrode and the second dissolved oxygen electrode, and the driving device (5) is used for driving the water sample in the detection channel (1) to flow.
2. The rapid BOD detection in-situ biofilm carrier reactor according to claim 1, wherein the in-situ biofilm assembly (4) comprises a first cavity (401), a first filter membrane (402), a first feeding assembly (403) and a first receiving assembly (404), the first cavity (401) is communicated with the detection channel (1) through the first filter membrane (402), one end of the first cavity (401) is communicated with the first feeding assembly (403), the other end of the first cavity (401) is communicated with the first receiving assembly (404), the first feeding assembly (403) selectively provides nutrient medium into the first cavity (401), and the first receiving assembly (404) is used for accommodating the nutrient medium discharged from the first cavity (401).
3. The rapid BOD detection in situ biofilm carrier reactor of claim 2, wherein the first feed assembly (403) comprises a substrate storage tank (4031), a first feed pump (4032) and a first valve (4033), the substrate storage tank (4031) is communicated with one end of the first chamber (401) through the feed pump (4032) and the first valve (4033) in sequence, the first receiving assembly (404) comprises a first waste tank (4041) and a second valve (4042), and the other end of the first chamber (401) is communicated with the first waste tank (4041) through the second valve (4042).
4. The rapid BOD detection in situ bio-membrane carrier reactor as claimed in claim 2, further comprising a second chamber (405), a second filter (406), a second feeding module (407) and a second receiving module (408), wherein the second chamber (405) is connected to the detection channel (1) through the second filter (406), the first chamber (401) is connected to the second chamber (405) through the first filter (402), one end of the second chamber (405) is connected to the second feeding module (407), the other end of the second chamber (405) is connected to the second receiving module (408), the second feeding module (407) selectively provides the cleaning solution to the second chamber (405), and the second receiving module (408) is used for receiving the cleaning solution and the micro-organisms discharged from the second chamber (405).
5. The fast BOD detection in-situ biofilm carrier reactor according to claim 4, wherein the second feeding assembly (407) comprises a cleaning solution storage tank (4071), a second feeding pump (4072) and a third valve (4073), the cleaning solution storage tank (4071) is communicated with one end of the second cavity (405) through the second feeding pump (4072) and the third valve (4073) in turn, the second receiving assembly (408) comprises a second waste tank (4081) and a fourth valve (4082), and the other end of the second cavity (405) is communicated with the second waste tank (4081) through the fourth valve (4082).
6. The rapid BOD detection in-situ bio-membrane carrier reactor as claimed in claim 3, further comprising a third cavity (409), a third filter membrane (410), a third feeding component (411) and a third receiving component (412), the third cavity (409) is communicated with the detection channel (1) through a third filter membrane (410), the second cavity (405) is communicated with the third cavity (409) through a second filter membrane (406), the first cavity (401) is communicated with the second cavity (405) through a first filter membrane (402), one end of the third cavity (409) is communicated with a third feeding component (411), the other end of the third cavity (409) is communicated with a third receiving component (412), the third feeding component (411) selectively provides gel with selective permeability into the third cavity (409), and the third receiving component (412) is used for containing gel discharged from the third cavity (409).
7. The rapid BOD detection in situ biofilm carrier reactor according to claim 6, wherein the third feed assembly (411) comprises a gel storage tank (4111), a third feed pump (4112) and a fifth valve (4113), the gel storage tank (4111) is communicated with one end of the third cavity (409) through the third feed pump (4112) and the fifth valve (4113) in turn, the third receiving assembly (412) comprises a third waste material tank (4121) and a sixth valve (4122), and the other end of the third cavity (409) is communicated with the third waste material tank (4121) through the sixth valve (4122).
8. The fast BOD detection in situ biofilm carrier reactor according to claim 7, wherein the third feed assembly (411) further comprises a gel valve (4114), a rinse tank (4115) and a rinse valve (4116), the gel tank (4111) being in communication with the third feed pump (4112) via the gel valve (4114), and the rinse tank (4115) being in communication with the conduit between the gel valve (4114) and the third feed pump (4112) via the rinse valve (4116).
9. The rapid BOD detection in situ biofilm carrier reactor according to claim 1, wherein the driving means (5) is a water pump.
10. The rapid BOD detection in-situ biofilm carrier reactor according to claim 1, further comprising an aerator (6), wherein the end of the detection channel (1) far away from the driving device (5) is provided with the aerator (6).
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