CN113485019A - Gaussian-like flat-top beam shaping system for flow cytometer - Google Patents
Gaussian-like flat-top beam shaping system for flow cytometer Download PDFInfo
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- CN113485019A CN113485019A CN202110862426.8A CN202110862426A CN113485019A CN 113485019 A CN113485019 A CN 113485019A CN 202110862426 A CN202110862426 A CN 202110862426A CN 113485019 A CN113485019 A CN 113485019A
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- collimator
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/09—Beam shaping, e.g. changing the cross-sectional area, not otherwise provided for
- G02B27/0927—Systems for changing the beam intensity distribution, e.g. Gaussian to top-hat
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/09—Beam shaping, e.g. changing the cross-sectional area, not otherwise provided for
- G02B27/0938—Using specific optical elements
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/09—Beam shaping, e.g. changing the cross-sectional area, not otherwise provided for
- G02B27/0938—Using specific optical elements
- G02B27/095—Refractive optical elements
- G02B27/0955—Lenses
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/28—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00 for polarising
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Abstract
The invention discloses a quasi-Gaussian flat-top beam shaping system for a flow cytometer, which belongs to the technical field of laser beam shaping and comprises a collimator, at least one group of decomposition mechanisms and a focusing lens, wherein the decomposition mechanisms and the focusing lens are positioned at the same side of the collimator, the light incident surface of each decomposition mechanism is parallel to the light emergent surface of the collimator, each decomposition mechanism comprises a half wave plate and a birefringent crystal, and the half wave plate is positioned between the birefringent crystal and the collimator; the invention uses the half wave plate, the double refraction crystal and the focusing lens to orderly arrange, shapes the Gaussian beam emitted from the collimator into a focusing light spot which is flat in one direction and Gaussian in one direction, has simple structure, reduces the manufacturing cost, changes the wedge angle direction of the wedge double refraction crystal to change the direction of the flat beam, and uses the multilayer decomposition mechanism to decompose and focus the beam for multiple times, thereby changing the width of the light spot and expanding the working range.
Description
Technical Field
The invention belongs to the technical field of laser beam shaping, and particularly relates to a Gaussian-like flat-top beam shaping system for a flow cytometer.
Background
The laser light source is one of the core components of the flow cytometer, and the flow cytometer with high resolution and high flux puts higher requirements on the distribution of laser beams. In order to realize high resolution, the laser beam is required to have narrow light intensity distribution and high central intensity in the flow direction, and the laser beam is required to have wide and uniform light intensity distribution in the direction perpendicular to the flow direction, that is, one direction is required to be gaussian distribution, and the other direction is required to be flat-top light output.
At present, the main method for shaping the Gaussian beam into the flat-top light output is to adopt an aspheric lens or a diffraction optical element and the like, the key elements adopted by the schemes have higher cost, and other optical elements are required to be added for obtaining the light spot meeting the requirement, so the structure is complex, and the assembly difficulty is high.
Disclosure of Invention
The invention aims to: the system for shaping the Gaussian flat-top beam is used for a flow cytometer, and aims to solve the problems that when the Gaussian beam is shaped into the flat-top beam, a plurality of optical elements are needed, so that the light spot action range is limited, the structure is complex, and the manufacturing cost is high.
In order to achieve the purpose, the invention adopts the following technical scheme: a quasi-Gaussian flat-top beam shaping system for a flow cytometer comprises a collimator, at least one group of decomposition mechanisms and a focusing lens, wherein the decomposition mechanisms and the focusing lens are positioned on the same side of the collimator, the light incident surface of each decomposition mechanism is parallel to the light emergent surface of the collimator, each decomposition mechanism comprises a half wave plate and a birefringent crystal, the half wave plate is positioned between the birefringent crystal and the collimator, and the half wave plate is positioned between the focusing lens and the collimator.
As a further description of the above technical solution:
the decomposing mechanism is arranged in a group, and the half wave plate and the focusing lens are respectively positioned at two opposite sides of the birefringent crystal.
As a further description of the above technical solution:
the decomposing mechanism is arranged in a group, and the focusing lens is positioned between the half wave plate and the birefringent crystal.
As a further description of the above technical solution:
the decomposition mechanisms are arranged in two groups, and the two groups of decomposition mechanisms are positioned between the focusing lens and the collimator.
As a further description of the above technical solution:
the birefringent crystal is wedge-shaped.
As a further description of the above technical solution:
the light incident surface of the focusing lens is parallel to the light emergent surface of the collimator.
In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:
in the invention, the half-wave plate, the birefringent crystal and the focusing lens are orderly arranged to shape the Gaussian beam emitted from the collimator into a focusing light spot which is flat-topped in one direction and is Gaussian-shaped in one direction, the structure is simple, the manufacturing cost is reduced, the direction of the flat-topped beam is changed by changing the direction of the wedge angle of the wedge birefringent crystal, the beam is decomposed and focused for multiple times by utilizing the multilayer decomposition mechanism, the width of the light spot is changed, and the working range is expanded.
Drawings
Fig. 1 is a schematic structural diagram of an embodiment of a gaussian-like flat-top beam shaping system for a flow cytometer.
Fig. 2 is a schematic structural diagram of an embodiment two of a gaussian-like flat-top beam shaping system for a flow cytometer.
FIG. 3 is a schematic diagram of a third embodiment of a Gaussian-like flat-top beam shaping system for use in a flow cytometer.
Fig. 4 shows quasi-gaussian flat-top spot shapes obtained by using the first embodiment and the second embodiment.
Fig. 5 is a quasi-gaussian flat-top spot shape obtained using the third embodiment.
Illustration of the drawings:
1. a collimator; 2. a disassembly mechanism; 21. a half wave plate; 22. a birefringent crystal; 3. a focusing lens.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-5, the present invention provides a technical solution: a quasi-Gaussian flat-top beam shaping system for a flow cytometer comprises a collimator 1, at least one set of decomposition mechanism 2 and a focusing lens 3, wherein the decomposition mechanism 2 and the focusing lens 3 are positioned on the same side of the collimator 1, the light incident surface of the decomposition mechanism 2 is parallel to the light emergent surface of the collimator 1, the decomposition mechanism 2 comprises a half wave plate 21 and a birefringent crystal 22, the half wave plate 21 is positioned between the birefringent crystal 22 and the collimator 1, and the half wave plate 21 is positioned between the focusing lens 3 and the collimator 1;
the decomposition mechanism 2 is set as a group, the half wave plate 21 and the focusing lens 3 are respectively positioned at two opposite sides of the birefringent crystal 22, the collimator 1 forms the light output from the laser diode or other lasers into light spots which are collimated and output according to the light spot proportion required by the flow cytometer, the distribution of the light spots is Gaussian distribution at this time, the half wave plate 21 rotates the polarization direction of the light spots output by the collimator 1 to the direction which forms 45 degrees with the incident surface of the birefringent crystal 22, the light spots in the Gaussian distribution are decomposed into P light and S light by the birefringent crystal 22, the included angle between the P light and the S light is theta, the focal length of the focusing lens 3 is f, and the focusing lens 3 converges the light spots to the focal point, the light spot with one direction of flat-top light beam and the size of light spot as a and the other direction as Gaussian light beam can be obtained, and the following relations are satisfied by theta, f and a:
the decomposition mechanism 2 is arranged in a group, the focusing lens 3 is positioned between the half wave plate 21 and the birefringent crystal 22, the collimator 1 forms light output from a laser diode or other lasers into light spots which are collimated and output according to the light spot proportion required by the flow cytometer, the distribution of the light spots is Gaussian distribution at the moment, the half wave plate 21 rotates the polarization direction of the light spots output by the collimator 1 to the direction which forms an angle of 45 degrees with the incident surface of the focusing lens 3, the focusing lens 3 converges the light spots to the focal point, the light spots in the Gaussian distribution are decomposed into P light and S light by the birefringent crystal 22, the P light and the S light form certain deviation, so that the light spots with a flat-top light beam and a light spot size of a in one direction and a Gaussian light beam in the other direction can be obtained, and the deviation amounts delta and a meet the following relation
The two groups of decomposition mechanisms 2 are arranged, the two groups of decomposition mechanisms 2 are positioned between the focusing lens 3 and the collimator 1, the collimator 1 forms light output by a laser diode or other lasers into light spots which are output in a light spot proportion collimation mode according to the requirements of a flow cytometer, the light spots are distributed in a Gaussian distribution mode at the moment, the first group of decomposition mechanisms 2 act, the half wave plate 21 rotates the polarization direction of the light spots output by the collimator 1 to the direction which forms an angle of 45 degrees with the incident plane of the birefringent crystal 22, and the light spots in the Gaussian distribution mode are decomposed into light spots with an included angle of theta through the birefringent crystal 221And two light beams with the same intensity are decomposed into two light beams with an included angle theta through the birefringent crystal 22 under the action of the second group of decomposition mechanisms 22And four paths of light with the same intensity are focused by the focusing lens 3, the focusing lens 3 converges the light spots to the focus, so that the light spots of two directions of flat-topped light beams with the light spot size of a and two other directions of Gaussian light beams, theta, can be obtained1、θ2F and a satisfy the following relationships:
the birefringent crystal 22 is wedge-shaped, and the direction of the wedge angle of the wedge is consistent with the direction of the beam shaped as the flat top;
the light incident surface of the focusing lens 3 is parallel to the light emergent surface of the collimator 1.
The working principle is as follows: firstly, the collimator 1 shapes the light output from the laser diode or other lasers into the light spot which is collimated and output according to the light spot proportion required by the flow cytometer, at the moment, the distribution of the light spot is Gaussian distribution, secondly, the half wave plate 21 in the decomposition mechanism 2 has a rotation effect on the polarization direction of the light spot, then, when the focusing lens 3 is positioned in the decomposition mechanism 2, the focusing lens 3 focuses the light spot firstly, the birefringent crystal 22 decomposes the focused light spot, and finally, when the focusing lens 3 is positioned at one side of the decomposition mechanism 2, the birefringent crystal 22 decomposes the light spot, and the decomposed light beam is focused by the focusing lens 3.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (6)
1. A gaussian-like flat-top beam shaping system for a flow cytometer, comprising: including collimator (1), at least a set of decomposition mechanism (2) and focusing lens (3), decomposition mechanism (2) with focusing lens (3) are located same one side of collimator (1), the income plain noodles of decomposition mechanism (2) is on a parallel with the play plain noodles of collimator (1), decomposition mechanism (2) include half wave plate (21) and birefringent crystal (22), half wave plate (21) are located birefringent crystal (22) with between collimator (1), just half wave plate (21) are located focusing lens (3) with between collimator (1).
2. A gaussian-like flat top beam shaping system for a flow cytometer as described in claim 1, wherein the resolving mechanism (2) is provided as a set, and the half wave plate (21) and the focusing lens (3) are located on opposite sides of the birefringent crystal (22).
3. A gaussian-like flat top beam shaping system for a flow cytometer as described in claim 1, wherein the resolving mechanism (2) is provided as a set, and the focusing lens (3) is located between the half wave plate (21) and the birefringent crystal (22).
4. A gaussian-like flat top beam shaping system for a flow cytometer as described in claim 1, wherein said resolving mechanisms (2) are arranged in two groups, two groups of said resolving mechanisms (2) being located between said focusing lens (3) and said collimator (1).
5. The gaussian-like flat-top beam shaping system for a flow cytometer of claim 1, wherein the birefringent crystal (22) is wedge-shaped.
6. The gaussian-like flat-top beam shaping system for flow cytometry according to claim 1, wherein the light incident surface of the focusing lens (3) is parallel to the light emergent surface of the collimator (1).
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110862426.8A CN113485019A (en) | 2021-07-29 | 2021-07-29 | Gaussian-like flat-top beam shaping system for flow cytometer |
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| CN202110862426.8A CN113485019A (en) | 2021-07-29 | 2021-07-29 | Gaussian-like flat-top beam shaping system for flow cytometer |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114077066A (en) * | 2021-10-13 | 2022-02-22 | 深圳朗光科技有限公司 | Beam expanding collimator |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114077066A (en) * | 2021-10-13 | 2022-02-22 | 深圳朗光科技有限公司 | Beam expanding collimator |
| CN114077066B (en) * | 2021-10-13 | 2023-09-29 | 深圳朗光科技有限公司 | Beam-expanding collimator |
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