CN113475425B - In ovo injection device for immunization or nutrition supply of early embryos of poultry animals - Google Patents
In ovo injection device for immunization or nutrition supply of early embryos of poultry animals Download PDFInfo
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- CN113475425B CN113475425B CN202110569788.8A CN202110569788A CN113475425B CN 113475425 B CN113475425 B CN 113475425B CN 202110569788 A CN202110569788 A CN 202110569788A CN 113475425 B CN113475425 B CN 113475425B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K45/00—Other aviculture appliances, e.g. devices for determining whether a bird is about to lay
- A01K45/007—Injecting or otherwise treating hatching eggs
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06N—COMPUTING ARRANGEMENTS BASED ON SPECIFIC COMPUTATIONAL MODELS
- G06N20/00—Machine learning
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/70—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in livestock or poultry
Abstract
The invention discloses an in ovo injection device, in particular to an in ovo injection device for early embryo immunization or nutrition supply of poultry animals, belonging to the technical field of agricultural animal production and life science research. Provides an in ovo injection device for the immunization or nutrition supply of early embryos of poultry animals, which can carry out the medicine injection to the chick embryos of different ages in days and different parts of the chick embryos. The in ovo injection device comprises a supporting system, a fixing system and an injection system, and further comprises a light induction recognition system, a position adjusting and aligning system and a control center, wherein the injection part of the poultry hatching egg fixed between the supporting system and the fixing system is recognized through the light induction recognition system under the coordination of the control center, and the recognized injection part is aligned with the injection system along the vertical direction through the position adjusting and aligning system under the coordination of the control center and the supporting system.
Description
Technical Field
The invention relates to an in ovo injection device, in particular to an in ovo injection device for early embryo immunization or nutrition supply of poultry animals, belonging to the technical field of agricultural animal production and life science research.
Background
In the existing agricultural animal husbandry production, the production of oviparous animals such as chickens, ducks and geese has obvious characteristics or problems:
1. the newborn chicks cannot generate antibodies by themselves, are only protected by maternal antibodies, have low immunity, are easy to infect lethal bacteria or viruses, and pose a great threat to agricultural production and human and animal health.
2. The chick embryo or in-ovo injection technology is mainly used for improving the immunity of newborn chicks, and vaccines or nutrient substances are injected into tissues such as chick embryos before shelling or yolk sacs, so that the immunity of the chick embryos is improved, and the development and maturation speed of the immune organs of the chick embryos is accelerated.
3. The existing industrialized chick embryo injection device is mainly used for inoculating various chick high-risk disease vaccines such as Marek vaccine, infectious bursal disease vaccine and the like, the injection mode is vertical injection at a big-end air chamber end, and the injection part is chick embryo neck muscle.
Modern industrial chick embryo injection devices typically include a bottom fixture and a top injection device. The bottom fixing device is composed of a fixing support and a common egg support and plays a role in fixing hatching eggs. The top injection device comprises: an outer sleeve of the injection needle cylinder, an inner injection needle cylinder, the injection needle, a needle cylinder pushing device and a needle cylinder liquid pushing device. The injection needle is connected with an internal injection needle cylinder, and the internal injection needle cylinder is internally provided with a medicament to be injected. The upper part of the inner syringe is connected with a syringe pushing device, the upper part of the liquid storage bin of the inner syringe is connected with a syringe liquid pushing device, and the outer part of the inner syringe is provided with a syringe sleeve with a protective effect. The entire upper syringe barrel is connected to an external push-pull device.
The in ovo injection is performed according to the following using process:
1. selecting hatching eggs which are 18 days old to be hatched, putting the hatching eggs into a bottom fixing device, and placing the big-head air chamber end upwards;
2. the top injection device moves downwards, and the protection rubber pad below the outer sleeve of the injection syringe contacts with the eggshell and then stops;
3. the inner injection needle cylinder is pushed by the needle cylinder power device to move down rapidly to drive the injection needle below to puncture the eggshell, the air chamber membrane and the amnion, and finally the injection needle punctures the neck muscle of the chick embryo;
4. then the liquid propeller in the needle cylinder slowly pushes the medicine in the needle cylinder;
5. after the medicine is pushed, the syringe power device moves upwards to drive the internal injection syringe and the injection needle to return to the original position, and then the whole top injection device moves upwards to return to the original position;
6. the operator takes out the hatching eggs after injection, and seals the needle holes at the large end after injection by melted paraffin or electric melting glue to prevent bacterial infection.
In ovo injections using the above-described devices have the following problems,
1. the existing industrialized chick embryo injection device adopts a chick embryo intramuscular injection method, can only inject small dose of medicine, such as 1-200 ul of vaccine, and cannot effectively inject large dose of medicine, such as nutrient solution with the dosage exceeding 200 ul;
2. large dose of medicine needs to be injected through yolk sac, the position of embryo and yolk sac can not be distinguished by the existing industrialized chick embryo injection device, and only embryo intramuscular injection can be carried out;
3. the existing industrialized chick embryo injection device does not have an egg irradiation device, can not observe the position of the chick embryo, can not automatically acquire the images of different injection parts such as the embryo, the yolk sac, the amniotic cavity, the allantois and the like, can only acquire the images by artificial irradiation of eggs, and has large workload;
4. the existing industrialized chick embryo injection device has no image recognition device, and can not automatically recognize and distinguish different injection parts such as embryos, yolk sacs, amniotic cavities, allantois and the like;
5. the embryo positions of the chicken embryos of different hatching eggs are different, and the error of the injection position can be caused by blind vertical injection through the big-head air chamber end; the vaccine should be injected into embryo muscle but injected into amniotic cavity or yolk sac, so that the injected vaccine is ineffective, or blood vessels in the amniotic cavity or yolk sac cavity are punctured, so that embryo bleeding is caused, and chick embryo dysgenesis or chick embryo death is caused;
6. the existing industrialized chick embryo injection device adopts an intramuscular injection mode, and can only inject the basically formed embryo which develops at the age of 16-18 days after hatching; when the 21-day-old chick embryos are out of the shell, the injection time is late, the action time of the medicine is insufficient, and the embryo injection effect is poor.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: provides an in ovo injection device for the immunization or nutrition supply of early embryos of poultry animals, which can carry out the medicine injection to the chick embryos of different ages in days and different parts of the chick embryos.
The technical scheme adopted for solving the technical problems is as follows: an in ovo injection device for immunization or nutrition supply of early embryos of poultry animals comprises a supporting system, a fixing system and an injection system, and further comprises a light induction recognition system, a position adjusting and aligning system and a control center, wherein injection parts of hatching eggs of the poultry animals, which are fixed between the supporting system and the fixing system, are recognized under the coordination of the control center through the light induction recognition system, and the recognized injection parts are aligned with the injection system along the vertical direction under the coordination of the control center and the supporting system through the position adjusting and aligning system.
Furthermore, the control center comprises a central control module containing an AI image recognition chip, the supporting system, the fixing system, the injection system and the position adjusting and aligning system are respectively and simultaneously connected with the central control module, and the light induction recognition system is connected with the AI image recognition chip.
The preferable mode of the scheme is that the light induction identification system comprises an LED highlight lamp and a photosensitive element, the photosensitive element is connected with an AI image identification chip of the control center, and embryos, yolk sacs, amniotic cavities and allantois in the poultry hatching eggs are identified and determined by the LED highlight lamp under the cooperation of the photosensitive element.
Further, the LED high-light lamps are arranged on the bottom and the side wall of the supporting system, light through holes matched with the corresponding LED high-light lamps are arranged at corresponding positions of the bottom and the side wall of the supporting system, and the photosensitive element is arranged at the lower end of the fixing system.
The preferred mode of the above scheme is that the photosensitive element comprises a photosensitive element body, a photosensitive element fixing shell and a photosensitive element push-pull rod, the photosensitive element body arranged at the lower end of the fixing system through the photosensitive element fixing shell is connected with the photosensitive element push-pull rod, a data line of the photosensitive element push-pull rod is connected with the central control module, and the data line of the photosensitive element body is connected with the AI image recognition chip.
Furthermore, the position adjusting and aligning system comprises a guide sliding groove with a driving mechanism, the supporting system can horizontally move and/or swing under the matching of the driving mechanism and the control center through the guide sliding groove, and a data line of the driving mechanism is connected with the central control module.
The injection system is movably arranged in the pushing sleeve along the vertical direction, the photosensitive element fixing shell is arranged on the pushing sleeve above the protective rubber pad along the horizontal direction, and a data line of the large crimping push rod group is connected with the central control module.
Further, the supporting system comprises a bracket and an egg support, the egg support is arranged below the pushing sleeve in a position corresponding to the fixing system through the bracket, and the lower end of the bracket is arranged in the guide sliding groove.
Further, the injection system comprises a small injection push rod, an injection needle cylinder and an injection needle, wherein the injection needle is arranged at the lower end of the injection needle cylinder, the upper end of the injection needle cylinder is connected with the large crimping push rod group, and the power output end of the small injection push rod capable of reciprocating in the vertical direction is connected with the large crimping push rod group.
The invention has the beneficial effects that: the application provides an in ovo injection apparatus is on current braced system, fixing system and injection system's basis, through increasing to set up photoinduction identification system, position adjustment and to system and control center well to the messenger fixes the injection position of the poultry hatching egg between braced system and fixing system and passes through photoinduction identification system discerns under control center's cooperation, then through position adjustment with the injection position of discerning well the system align along vertical direction with injection system under control center and braced system's cooperation. The problem of large workload caused by the fact that images of different injection parts such as embryos, yolk sacs, amniotic cavities, allantois and the like cannot be automatically acquired and only information can be acquired by manually irradiating eggs in the prior art is solved, and meanwhile, automatic injection work can be realized through the cooperation of a control center, and the injection quality is ensured.
Drawings
FIG. 1 is a schematic diagram of the structure of an in ovo injection apparatus for immunization or nutrition supplementation of early embryos of avian animals according to the present invention;
FIG. 2 is a schematic view of the injection with the position adjustment alignment system in a non-aligned position according to the present invention.
Labeled as: the device comprises a supporting system 1, a fixing system 2, an injection system 3, a light-induced identification system 4, a position adjusting and aligning system 5, a poultry hatching egg 6, a central control module 7, an LED highlight lamp 8, a photosensitive element 9, a photosensitive element body 10, a photosensitive element fixing shell 11, a photosensitive element push-pull rod 12, a crimping large push rod group 13, a protective rubber pad 14, a pushing sleeve 15, a support 16, an egg support 17, an injection small push rod 18, an injection needle cylinder 19 and an injection needle 20.
Detailed Description
As shown in figures 1 and 2, the invention provides an in ovo injection device for immunization or nutrition supply of early embryos of poultry animals, which can inject medicaments to different day-old chick embryos and different parts of the chick embryos. In ovo injection apparatus include braced system 1, fixing system 2 and injection system 3, its characterized in that: the in ovo injection device further comprises a light induction recognition system 4, a position adjusting and aligning system 5 and a control center, wherein the injection part of the poultry hatching egg 6 fixed between the supporting system 1 and the fixing system 2 is recognized through the light induction recognition system 4 under the coordination of the control center, and the recognized injection part is aligned with the injection system 3 along the vertical direction through the position adjusting and aligning system 5 under the coordination of the control center and the supporting system 1. The application provides an in ovo injection apparatus is on current braced system, fixed system and injection system's basis, through increasing to set up photoinduction identification system, position adjustment and to system and control center to make the injection position of fixing the poultry hatching egg between braced system and fixed system pass through photoinduction identification system discerns under control center's cooperation, then the injection position that will discern aligns system and injection system along vertical direction under control center and braced system's cooperation through position adjustment. The problem of large workload caused by the fact that images of different injection parts such as embryos, yolk sacs, amniotic cavities, allantois and the like cannot be automatically acquired and only information can be acquired by manually irradiating eggs is solved, and meanwhile, automatic injection work can be realized by the cooperation of a control center, so that the injection quality is ensured.
In the above embodiment, in order to simplify the structure of each component of the in ovo injection device of the present application as much as possible and to adopt common components as much as possible, so as to reduce the difficulty of manufacturing the device and facilitate subsequent injection operations, the control center of the present application includes a central control module 7 including an AI image recognition chip, the support system 1, the fixing system 2, the injection system 3 and the position adjusting and aligning system 5 are respectively and simultaneously connected to the central control module 7, and the light sensing recognition system 4 is connected to the AI image recognition chip. Correspondingly, the light induction identification system 4 comprises an LED highlight lamp 8 and a light induction element 9, the light induction element 9 is connected with an AI image identification chip of a control center, and embryos, yolk sacs, amniotic cavities and allantois in the poultry hatching eggs 6 are identified and determined by the LED highlight lamp 8 under the cooperation of the light induction element 9. Further, the bottom and the side wall of the support system 1 are both provided with the LED floodlights 8, the corresponding positions of the bottom and the side wall of the support system are both provided with light through holes adapted to the corresponding LED floodlights 8, and the photosensitive element 9 is arranged at the lower end of the fixing system 2. At this time, the photosensitive element 1 generally needs to include a photosensitive element body 10, a photosensitive element fixing shell 11 and a photosensitive element push-pull rod 12, the photosensitive element body 10 arranged at the lower end of the fixing system 1 through the photosensitive element fixing shell 11 is connected to the photosensitive element push-pull rod 12, a data line of the photosensitive element push-pull rod 12 is connected to the central control module 7, and a data line of the photosensitive element body 10 is connected to the AI image recognition chip.
Correspondingly, the position adjusting and aligning system 5 comprises a guide sliding groove with a driving mechanism, the supporting system 1 can horizontally move and/or swing through the guide sliding groove under the matching of the driving mechanism and a control center, and a data line of the driving mechanism is connected with the central control module 7. At this moment, combine the structural feature of current in ovo injection apparatus, fixing system 2 of this application also include crimping big push rod group 13, protection cushion 14 and bulldoze sleeve pipe 15, protection cushion 14 arrange the lower extreme that bulldozes sleeve pipe 15, crimping big push rod group 13 arrange the upper portion that bulldozes sleeve pipe 15, injection system 3 along the movable arrangement of vertical direction bulldoze in sleeve pipe 15, photosensitive element set casing 11 arrange on the sleeve pipe 15 that bulldozes above protection cushion 14 along the horizontal direction, the data line of crimping big push rod group 13 is connected with central control module. The supporting system 1 comprises a bracket 16 and an egg tray 17, the egg tray 17 is arranged below the push sleeve 15 through the bracket 16 in a position corresponding to the fixing system 2, and the lower end of the bracket 16 is arranged in the guide sliding groove. Correspondingly, the injection system 3 comprises an injection small push rod 18, an injection needle cylinder 19 and an injection needle 20, wherein the injection needle 20 is arranged at the lower end of the injection needle cylinder 19, the upper end of the injection needle cylinder 19 is connected with the crimping large push rod group 13, and the power output end of the injection small push rod 18 capable of reciprocating in the vertical direction is connected with the crimping large push rod group 13.
In conclusion, the in ovo injection device improved by the application is characterized in that the photosensitive element with the glass protective layer, the photosensitive element fixing shell and the electric cylinder push-pull device connected with the photosensitive element are added in the top injection device; the LED strong light and the corresponding light through hole are arranged in the egg support of the bottom supporting device, the support of the bottom supporting device is changed into a barrel structure and is connected with the egg support, and then the side LED strong light and the corresponding side light through hole are arranged in the support. A sliding device is connected below the egg support and can translate on the corresponding sliding groove, so that the inclination angle and the injection site of the hatching egg are changed; and an external AI image recognition chip is arranged on the injection device and used for receiving image signals from the photosensitive element and controlling the needle cylinder push-pull device, the push-pull device connected with the photosensitive element and the bottom sliding device. After the improvement, the in ovo injection device has the following advantages:
1. because the top injection device of the device contains a photosensitive element, after the bottom support device contains an LED strong light lamp to illuminate eggs, the device can automatically collect the illuminated egg images of different injection parts such as embryos, yolk sacs, amniotic cavities, allantois and the like.
2. Because the external connection AI image recognition chip, can automatic identification and distinguish different injection sites such as embryo, yolk sac, amniotic cavity, allantois, etc.
3. By automatically and accurately identifying the injection site, ineffective injection can be avoided, and bleeding or death of the chick embryo caused by blind injection can be avoided.
4. The method can automatically and accurately identify small embryos which are not completely formed during the age of 10-18 days, distinguish different injection parts such as yolk sacs, amniotic cavities, allantois and the like, advance the in ovo injection time and enhance the action time of injected medicaments.
Example one
The in ovo injection device of the invention has the following use procedures:
1. selecting hatching eggs which are hatched for 10-18 days, putting the hatching eggs into a bottom fixing device, wherein the small end is upward, and the large end air chamber is downward, as shown in the figures 1 and 2;
2. an LED strong light in the bottom supporting device is turned on to irradiate hatching eggs, a closed photosensitive element in the top injection device collects image of the irradiated eggs, and then the image is transmitted to an external AI image recognition chip through a data line, as shown in figures 1 and 2;
3. the AI image recognition chip distinguishes the embryo, the blood vessel and the yolk sac positions in the image by utilizing the set deep learning image recognition model, as shown in figures 1 and 2;
4. the central position of the acquired image is the corresponding position of the injection needle, if the injection needle is not over against the scheduled injection part, such as the yolk sac, the AI image recognition chip transmits data to the bottom sliding device, as shown in figures 1 and 2;
5. after receiving the signal from the AI image recognition chip, the bottom sliding device drives the whole fixing device to move obliquely left and right on the sliding groove, so as to adjust the inclination of the hatching eggs, as shown in fig. 1 and 2;
6. after the position is adjusted, the light sensing element is closed to collect the image of the egg illuminated by the LED strong light again, as shown in fig. 1 and 2;
7. the AI image recognition chip confirms again whether the injection needle is facing the predetermined injection site, as shown in fig. 1 and 2;
8. after the AI image recognition chip confirms that the needle is right, the AI image recognition chip transmits data to an electric cylinder push-pull device which is arranged in the sleeve and controls the photosensitive element to slide, and the push-pull device drives the closed photosensitive element to move towards the interior of the sleeve to expose the injection needle, as shown in figures 1 and 2;
9. the AI image recognition chip transmits data to the top push-pull device, the top injection device moves downwards, the inner injection needle cylinder is pushed by the needle cylinder power device to move downwards quickly, and the lower injection is driven to be punctured into an injection part, such as a yolk sac;
10. then the liquid pusher in the syringe slowly pushes the medicine in the syringe, as shown in fig. 1 and 2;
11. after the medicine is pushed, the syringe power device moves upwards to drive the internal injection syringe and the injection needle to return to the original positions, then the whole top injection device moves upwards to return to the original positions, and the push-pull device which controls the photosensitive element in the sleeve pipe pushes the photosensitive element to move, close and return, as shown in fig. 1 and fig. 2;
12. the operator takes out the injected hatching eggs, and seals the needle hole at the small end after injection with melted paraffin or electric melting glue to prevent bacterial infection, as shown in fig. 1 and 2.
Claims (6)
1. An in ovo injection device for immunization or nutrition supplementation of early embryos of avian animals, comprising a support system (1), a fixation system (2) and an injection system (3), characterized in that: the in ovo injection device also comprises a light induction identification system (4), a position adjusting and aligning system (5) and a control center, the injection part of the poultry hatching egg (6) fixed between the support system (1) and the fixing system (2) is identified by the light induction identification system (4) under the coordination of the control center, the identified injection part is aligned with the injection system (3) along the vertical direction under the coordination of the control center and the support system (1) by the position adjusting and aligning system (5),
the light induction identification system (4) comprises an LED highlight lamp (8) and a light sensing element (9), the light sensing element (9) is connected with an AI image identification chip of a control center, embryos, yolk sacs, amniotic cavities and allantois in the poultry hatching eggs (6) are identified and determined by the LED highlight lamp (8) under the cooperation of the light sensing element (9),
the LED strong light lamps (8) are arranged on the bottom and the side wall of the supporting system (1), light through holes matched with the corresponding LED strong light lamps (8) are arranged at the corresponding positions of the bottom and the side wall of the supporting system, the photosensitive element (9) is arranged at the lower end of the fixing system (2),
the position adjusting and aligning system (5) comprises a guide sliding groove with a driving mechanism, the supporting system (1) can horizontally move and/or swing through the guide sliding groove under the matching of the driving mechanism and a control center, and a data line of the driving mechanism is connected with a central control module (7).
2. The in ovo injection apparatus for immunization or nutrition supplementation of avian animals as claimed in claim 1, wherein: the control center comprises a central control module (7) containing an AI image identification chip, the supporting system (1), the fixing system (2), the injection system (3) and the position adjusting and aligning system (5) are respectively and simultaneously connected with the central control module (7), and the light induction identification system (4) is connected with the AI image identification chip.
3. The in ovo injection apparatus for the immunization or nutritional supplementation of avian animals' early embryos according to claim 1 or 2, characterized in that: the photosensitive element (9) comprises a photosensitive element body (10), a photosensitive element fixing shell (11) and a photosensitive element push-pull rod (12), the photosensitive element body (10) arranged at the lower end of the fixing system (2) through the photosensitive element fixing shell (11) is connected with the photosensitive element push-pull rod (12), a data line of the photosensitive element push-pull rod (12) is connected with the central control module (7), and the data line of the photosensitive element body (10) is connected with the AI image recognition chip.
4. The in ovo injection apparatus for immunization or nutrition supplementation of avian animals as claimed in claim 3, wherein: the fixing system (2) comprises a crimping large push rod group (13), a protective rubber pad (14) and a pushing sleeve (15), the protective rubber pad (14) is arranged at the lower end of the pushing sleeve (15), the crimping large push rod group (13) is arranged at the upper part of the pushing sleeve (15), the injection system (3) is movably arranged in the vertical direction in the pushing sleeve (15), the photosensitive element fixing shell (11) is arranged on the pushing sleeve (15) above the protective rubber pad (14) in the horizontal direction, and a data line of the crimping large push rod group (13) is connected with the central control module.
5. The in ovo injection apparatus for the immunization or nutritional supplementation of avian animals' early embryos according to claim 4, characterized in that: the supporting system (1) comprises a bracket (16) and an egg tray (17), the egg tray (17) is arranged below the pushing sleeve (15) through the bracket (16) in a position corresponding to the fixing system (2), and the lower end of the bracket (16) is arranged in the guide sliding groove.
6. The in ovo injection apparatus for the immunization or nutritional supplementation of avian animals' early embryos according to claim 5, characterized in that: the injection system (3) comprises a small injection push rod (18), an injection needle cylinder (19) and an injection needle (20), wherein the injection needle (20) is arranged at the lower end of the injection needle cylinder (19), the upper end of the injection needle cylinder (19) is connected with the large crimping push rod group (13), and the power output end of the small injection push rod (18) capable of reciprocating in the vertical direction is connected with the large crimping push rod group (13).
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| CN202110569788.8A CN113475425B (en) | 2021-05-25 | 2021-05-25 | In ovo injection device for immunization or nutrition supply of early embryos of poultry animals |
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| US6244214B1 (en) * | 1999-01-06 | 2001-06-12 | Embrex, Inc. | Concurrent in ovo injection and detection method and apparatus |
| JP3442357B2 (en) * | 2000-08-25 | 2003-09-02 | 株式会社日立製作所 | Amphibian oocyte sample introduction device, amphibian oocyte sample introduction system, amphibian oocyte sample introduction method, amphibian oocyte production method, amphibian oocyte and method of selling or transferring it, as sensor for screening Method used, container, and analysis method |
| CN101341863B (en) * | 2008-09-02 | 2011-04-20 | 渤海大学 | Injection method for single-bird egg |
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